Lsi02G024220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G024220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Exopolygalacturonase-like
Location	chr02: 30821566 .. 30823703 (-)
RNA-Seq Expression	Lsi02G024220
Synteny	Lsi02G024220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAATGACAAGAAGTCATAGCCTCTTTCAAATCCTTCTCTTAATATTTGCTTGCCAATGTTGTGGAAAGGTTGCTGCCATTTTCAACGACGTCACCGGGGCTGCAAATCTCGTCGATGGGATCGTATCAACGGTAAATGATCAGCAGCTTCTTCGTCCTGCAGCAGCACCGCAACCTCTTGGTATCGGAACTCTTCCGGACGTCGGCGGCACGGTAAATGACATTAAGGCAAATGTTGATCTGAATGGGAATGGTGGTGCAGTTTTTGATTTTACAAAACATGGAGCTCAAGGTGATGGAAAGACTGATGATGCACAGGTGAGAATATCAAGCAAAACTTCAAGCTTTTAATTTGAACTAATTAAATTTAAACTTGTTAAATTATTTAAATTTGAGCCATAATTCTCAACCTACAACCTAAAAAAAATGTTTATGAAAAAAGGAAAGGCAAGAAGAGGTTGCCACTAGGCAAACTGCGGTTGGACTAAAAAAACATGAGTTAGTTTGAAAACCAATATAATTGCAATAATTTCTAGGGCTTCATTTTAATCATATATCTCTGCATGCTTTTTAAAAGATTTTAATTAGTTAACCATTTCAGAAATGGGAATATAGATCGTTAAGATTGCATTGTAGAACTTATAATTTGAATAATATTACACCAACCACAAGGTCATTTTTAGATATTAAATTTTACCATTATATAAAAATAAACCAATCACAAATTTTTGTTTGGTAGATATGAAGGGGTATAATTGTTTTGACATTTTTTCATCTTCTCTAACAAAAAAAAATAATATATTGATTTTCCACATGGCAGGCATTCATGACAACATGGATTGCAGCCGGAACACGGTCGGTCCAGCGAAGTTCTTTATTCCACAGGGCACATTTTCAGTGGGTCCGGTGACTTTCGCCGGTCCATGTAAAAGCTTCCCAATCACCCTCGAAAATCAAGGAACTGTAAAGGCCACAACTGATATAACTCAATATTCTTCTCCAGAATGGTTCTCCATTGAAGATATCATTGGCTTCATCCTCACCGGCTTCGGCGTTTTCGATGGCCAAGGCGCGGCTGCTTGGCCCTACAATAACTGCAAGAAAAACAACTTCTGTCAGAGTCTTCCAATCGTAACTATAATTTTACATTACTCAATCACTTCTCATTTAATTTTAAATTACAAGTTAAAACACTTGAAACTACCATAATTATGTCTAGTACATACCTGAACTTAACTAAAAAAAAAACAAGAATGGTTAAGAGACTAAATTATAATTGTGATTGATTAATTGATCTCTTTTTTTTGTAGTCGATTAAATTCTCGAGATTGAACCACACGATTGTTGATCGGCTCGCTTCACTAAACAGCAAGGGTTTTCACACATCTGTGTTCTACTGCTACAATTTTACTGCTACCAATGAACATTATAGCTCCAGGAAATAGTCCCAACACCGATGGAATGCACCTTAGCACCTCAAAATTGGTCACCATTACGAACAGTGTCATTGGCATTGGTGATGATTGTGTCTCCATTGGCCACGGTTGTGAGAAAGTCACTGTCACCAATGTCACTTGCGGCCCAGGACATGACCTCAGGTAATAGTCTTTCTTTTTTAAAAAGTTTAAATATTAAATAGACACAAAGAAACATCTTAAAGCTTTTTATTCTCTTTCAGCGTGGGTAGCTTGGGCAAGTACTCGAAAGAGAAGAGTATCTATGATGTTCTAGTAAAGAATTGCTCCATTTTCAATGTCACCAATGGTGCCAGAATCAAGACATGGGCTAGCCATATGTCGGGGTTAGCCTCGGGAATAATCTTCGAAGACGTTGTAATGTACAACGTCAAAAATCCTATAATTATCGATCAAACCCATGGCACTAGAGAGAAGAAGGTACATAGTAACAACAACAAAAACAAACTAATCGTGTTAATACTGATGAGTTTAAGCTTTTGAGTTCAGTAGTTATTTTAATAAAGCTACAACTAACCTTCCATGCAGGCTTCGAACTGGAAGATCAGCGACGTACGCTTCAAGAACATTCGAGGGACATCGACGACGACGTGGCAATGTTGTTGGAGTGCAGTGAGTTGCTTCCATGTAAGGGAGTGGAGCTTAGGGATATTAACTTGA

mRNA sequence

ATGACAATGACAAGAAGTCATAGCCTCTTTCAAATCCTTCTCTTAATATTTGCTTGCCAATGTTGTGGAAAGGTTGCTGCCATTTTCAACGACGTCACCGGGGCTGCAAATCTCGTCGATGGGATCGTATCAACGGTAAATGATCAGCAGCTTCTTCGTCCTGCAGCAGCACCGCAACCTCTTGGTATCGGAACTCTTCCGGACGTCGGCGGCACGGTAAATGACATTAAGGCAAATGTTGATCTGAATGGGAATGGTGGTGCAGTTTTTGATTTTACAAAACATGGAGCTCAAGGCATTCATGACAACATGGATTGCAGCCGGAACACGGTCGGTCCAGCGAAGTTCTTTATTCCACAGGGCACATTTTCAGTGGGTCCGGTGACTTTCGCCGGTCCATGTAAAAGCTTCCCAATCACCCTCGAAAATCAAGGAACTGTAAAGGCCACAACTGATATAACTCAATATTCTTCTCCAGAATGGTTCTCCATTGAAGATATCATTGGCTTCATCCTCACCGGCTTCGGCGTTTTCGATGGCCAAGGCGCGGCTGCTTGGCCCTACAATAACTGCAAGAAAAACAACTTCTCTCCAGGAAATAGTCCCAACACCGATGGAATGCACCTTAGCACCTCAAAATTGGTCACCATTACGAACAGTGTCATTGGCATTGGTGATGATTGTGTCTCCATTGGCCACGGTTGTGAGAAAGTCACTGTCACCAATGTCACTTGCGGCCCAGGACATGACCTCAGCGTGGGTAGCTTGGGCAAGTACTCGAAAGAGAAGAGTATCTATGATGTTCTAGTAAAGAATTGCTCCATTTTCAATGTCACCAATGGTGCCAGAATCAAGACATGGGCTAGCCATATGTCGGGGTTAGCCTCGGGAATAATCTTCGAAGACGTTGTAATGTACAACGTCAAAAATCCTATAATTATCGATCAAACCCATGGCACTAGAGAGAAGAAGGCTTCGAACTGGAAGATCAGCGACGTACGCTTCAAGAACATTCGAGGGACATCGACGACGACGTGGCAATGTTGTTGGAGTGCAGTGAGTTGCTTCCATGTAAGGGAGTGGAGCTTAGGGATATTAACTTGA

Coding sequence (CDS)

ATGACAATGACAAGAAGTCATAGCCTCTTTCAAATCCTTCTCTTAATATTTGCTTGCCAATGTTGTGGAAAGGTTGCTGCCATTTTCAACGACGTCACCGGGGCTGCAAATCTCGTCGATGGGATCGTATCAACGGTAAATGATCAGCAGCTTCTTCGTCCTGCAGCAGCACCGCAACCTCTTGGTATCGGAACTCTTCCGGACGTCGGCGGCACGGTAAATGACATTAAGGCAAATGTTGATCTGAATGGGAATGGTGGTGCAGTTTTTGATTTTACAAAACATGGAGCTCAAGGCATTCATGACAACATGGATTGCAGCCGGAACACGGTCGGTCCAGCGAAGTTCTTTATTCCACAGGGCACATTTTCAGTGGGTCCGGTGACTTTCGCCGGTCCATGTAAAAGCTTCCCAATCACCCTCGAAAATCAAGGAACTGTAAAGGCCACAACTGATATAACTCAATATTCTTCTCCAGAATGGTTCTCCATTGAAGATATCATTGGCTTCATCCTCACCGGCTTCGGCGTTTTCGATGGCCAAGGCGCGGCTGCTTGGCCCTACAATAACTGCAAGAAAAACAACTTCTCTCCAGGAAATAGTCCCAACACCGATGGAATGCACCTTAGCACCTCAAAATTGGTCACCATTACGAACAGTGTCATTGGCATTGGTGATGATTGTGTCTCCATTGGCCACGGTTGTGAGAAAGTCACTGTCACCAATGTCACTTGCGGCCCAGGACATGACCTCAGCGTGGGTAGCTTGGGCAAGTACTCGAAAGAGAAGAGTATCTATGATGTTCTAGTAAAGAATTGCTCCATTTTCAATGTCACCAATGGTGCCAGAATCAAGACATGGGCTAGCCATATGTCGGGGTTAGCCTCGGGAATAATCTTCGAAGACGTTGTAATGTACAACGTCAAAAATCCTATAATTATCGATCAAACCCATGGCACTAGAGAGAAGAAGGCTTCGAACTGGAAGATCAGCGACGTACGCTTCAAGAACATTCGAGGGACATCGACGACGACGTGGCAATGTTGTTGGAGTGCAGTGAGTTGCTTCCATGTAAGGGAGTGGAGCTTAGGGATATTAACTTGA

Protein sequence

MTMTRSHSLFQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQPLGIGTLPDVGGTVNDIKANVDLNGNGGAVFDFTKHGAQGIHDNMDCSRNTVGPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFILTGFGVFDGQGAAAWPYNNCKKNNFSPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTCGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFEDVVMYNVKNPIIIDQTHGTREKKASNWKISDVRFKNIRGTSTTTWQCCWSAVSCFHVREWSLGILT

Homology

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BLAST of Lsi02G024220 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.9e-55
Identity = 132/323 (40.87%), Postives = 163/323 (50.46%), Query Frame = 0


Query: 83  NGNGGAVFDFTKHGAQGIHDNMDCSRNTVG--------------PAKFFIPQGTFSVGPV 142
           N     V+D TK GA G     D S NT                PA   +P+GTF  GPV
Sbjct: 45  NSGNPTVYDITKFGAVG-----DGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPV 104

Query: 143 TFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFILTGFGVFDGQGAAAWPY 202
            FAGPCKS  +T+   GT+ ATT  + Y++PEWF  E +   +LTG G F G+G A W  
Sbjct: 105 IFAGPCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKA 164

Query: 203 NNCKK---------------------NNFS--------------------------PGNS 262
           + C K                     N  S                          P  S
Sbjct: 165 DGCGKKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAES 224

Query: 263 PNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTCGPGHDLSVGSLGKYSK 322
           PNTDG+HLS +  V+I +S I  GDDCVS+G G   VTV  V CGPGH LSVGSLGKY  
Sbjct: 225 PNTDGIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKN 284

Query: 323 EKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFEDVVMYNVKNPIIIDQTHGTR 345
           E+ +  + V NC++    NG RIKTW       A  I FE+++M +VKNPIIIDQ +G+R
Sbjct: 285 EEDVSGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSR 344

[Close]

BLAST of Lsi02G024220 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.5e-54
Identity = 117/317 (36.91%), Postives = 163/317 (51.42%), Query Frame = 0


Query: 85  NGGAVFDFTKHGAQGIHDNMDCSRN-------TVGPAKFFIPQGTFSVGPVTFAGPCKSF 144
           + G+VF+   +GA+G  D              + GP+   IP+G +++G V   GPCK  
Sbjct: 5   SSGSVFNVNDYGAKGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGS 64

Query: 145 PITLENQGTVKATTDITQYSSPEWFSIEDIIGFILTGFGVFDGQGAAAWPYNNCKKNN-- 204
            I  +  G VKA  D +++ S  W S   I G  ++G G  DGQG  AW  NNC KN   
Sbjct: 65  KIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNC 124

Query: 205 ---------------------------------------------FSPGNSPNTDGMHLS 264
                                                         +PG S NTDG+H+ 
Sbjct: 125 KHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVG 184

Query: 265 TSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTCGPGHDLSVGSLGKYSKEKSIYDVLV 324
            SK VTITN+ I  GDDC+SIG G + VT+T V CGPGH +S+GSLG+Y+ EK +  + V
Sbjct: 185 ISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITV 244

Query: 325 KNCSIFNVTNGARIKTWASHMSGLASGIIFEDVVMYNVKNPIIIDQ---THGTREKKA-S 344
           K C+     NG R+KTW +   G A+ + F+D+ M NV+NP+I+DQ    +G   ++A S
Sbjct: 245 KGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPS 304

[Close]

BLAST of Lsi02G024220 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.2e-53
Identity = 119/311 (38.26%), Postives = 162/311 (52.09%), Query Frame = 0


Query: 89  VFDFTKHGAQGIHDNMDCSRN-------TVGPAKFFIPQGTFSVGPVTFAGPCKSFPITL 148
           VFD TK+GA    D  +   N       +  P+   IP+GTF++  V   GPCKS P+ L
Sbjct: 26  VFDITKYGANSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKS-PLEL 85

Query: 149 ENQGTVKATTDITQYSSPEWFSIEDIIGFILTGFGVFDGQGAAAWP-------------- 208
           + Q T+KA +D +Q    EW ++  +  F ++G G+ DGQ AAAW               
Sbjct: 86  QIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAWECKQSKKCNKLPNNL 145

Query: 209 ------------------------YNNCKKNNF------SPGNSPNTDGMHLSTSKLVTI 268
                                    N CK   F      +P NSPNTDG+H+S S  V I
Sbjct: 146 SFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSVNI 205

Query: 269 TNSVIGIGDDCVSIGHGCEKVTVTNVTCGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFN 328
           T+S    GDDC+S+G   E++ +T VTCGPGH +SVGSLG    EK +  V V+NC+  N
Sbjct: 206 TDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTFTN 265

Query: 329 VTNGARIKTWASHMSGLASGIIFEDVVMYNVKNPIIIDQTHGTREK----KASNWKISDV 345
             NG RIKTW +   G+ + + FED+++ NV NP++IDQ +    K      S  KIS V
Sbjct: 266 TDNGVRIKTWPASHPGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVKISKV 325

[Close]

BLAST of Lsi02G024220 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 9.1e-52
Identity = 127/333 (38.14%), Postives = 169/333 (50.75%), Query Frame = 0


Query: 74  NDIKANVDLNGNGGAVFDFTKHGAQG---------IHDNMDCSRNTVGPAKFFIPQGTFS 133
           ND KA    +G GG+ FD TK GA G         + +    +    G     IP+G F 
Sbjct: 30  NDAKA----SGPGGS-FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFL 89

Query: 134 VGPVTFAGPCKSFPITLENQGTVKATTDITQYSS-PEWFSIEDIIGFILTGFGVFDGQGA 193
           VGP+ F GPCK   +T++  G + ATTD++QY     W  I  +   ++TG G  DGQG 
Sbjct: 90  VGPLNFTGPCKG-DVTIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGP 149

Query: 194 AAWPYNNCKK-----------------------------------------------NNF 253
           A W  N+C K                                               N  
Sbjct: 150 AVWSKNSCVKKYDCKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVT 209

Query: 254 SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTCGPGHDLSVGSL 313
           +PG+SPNTDG+H+  S  VTITN+VIG+GDDC+SIG G  KV +T VTCGPGH +S+GSL
Sbjct: 210 APGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSL 269

Query: 314 GKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGL-ASGIIFEDVVMYNVKNPIIID 345
           G+Y  EK + D+ VK+C++    NG RIK +    S L AS I +E++ M +   PIIID
Sbjct: 270 GRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIID 329

[Close]

BLAST of Lsi02G024220 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.2e-49
Identity = 103/284 (36.27%), Postives = 148/284 (52.11%), Query Frame = 0


Query: 110 TVGPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIG 169
           +V P+   IP+GT+ +  V   GPCK+ PI +  QGT++A  D + +  P W     +  
Sbjct: 59  SVTPSTVVIPKGTYLLSKVNLEGPCKA-PIEINVQGTIQAPADPSAFKDPNWVRFYSVEN 118

Query: 170 FILTGFGVFDGQGAAAWPYNNCKKNNF--------------------------------- 229
           F + G G+FDGQG+ A+  N C+   F                                 
Sbjct: 119 FKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINV 178

Query: 230 --------------SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTN 289
                         +P  SPNTDG+H+  S+ V I  S I  GDDC+SIG G + + +  
Sbjct: 179 FACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKE 238

Query: 290 VTCGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFED 343
           +TCGPGH +S+GSLGK+  E+ +  + + NC+I N +NGARIKTW     G  S I FED
Sbjct: 239 ITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFED 298

[Close]

BLAST of Lsi02G024220 vs. ExPASy TrEMBL
Match: A0A5A7U4W1 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00950 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 5.2e-143
Identity = 269/400 (67.25%), Postives = 295/400 (73.75%), Query Frame = 0


Query: 3   MTRSHSL-FQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQPL 62
           MTR+ SL  QILLL+FA QC  KVAA  +DVTG  NL+   V TVN +Q LRPA AP+ L
Sbjct: 1   MTRNLSLVIQILLLVFASQCSAKVAATLDDVTGTENLLKVTVPTVN-EQFLRPAGAPRRL 60

Query: 63  GIGTLPDVGGTVNDIKANVDLNGNGGAVFDFTKHGAQGIHDNMDCS----------RNTV 122
           GIGTLPD+G TVNDI+AN+ LN NGG+VFD TKHGA+   +  D            RNTV
Sbjct: 61  GIGTLPDIGSTVNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTV 120

Query: 123 GPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFI 182
           GPAKF IPQGT+ VGPVTFAGPCKSFPITLENQGTVKATTDI++YSSPEWFSIEDI GFI
Sbjct: 121 GPAKFLIPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFI 180

Query: 183 LTGFGVFDGQGAAAWPYNNCKKNNF----------------------------------- 242
           LTG GVFDGQG   WPYN+CKKN F                                   
Sbjct: 181 LTGSGVFDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFY 240

Query: 243 ------------SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVT 302
                       +P NSPNTDGMHLSTSKLVTITNSVIG GDDCVSIGH  E + VTNVT
Sbjct: 241 CYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVT 300

Query: 303 CGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFEDVV 345
           CGPGH LSVGSLGKYSKEKS+Y+VLVKNC+IFN TNGARIKTWAS +SGLASGIIFED+V
Sbjct: 301 CGPGHGLSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIV 360

[Close]

BLAST of Lsi02G024220 vs. ExPASy TrEMBL
Match: A0A5A7TQ89 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36913G00010 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 3.4e-142
Identity = 268/400 (67.00%), Postives = 294/400 (73.50%), Query Frame = 0


Query: 3   MTRSHSL-FQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQPL 62
           MTR+ SL  QILLL+FA QC  K AA  +DVTG  NL+   V TVN +Q LRPA AP+ L
Sbjct: 1   MTRNLSLVIQILLLVFASQCSAKDAATLDDVTGTENLLKVTVPTVN-EQFLRPAGAPRRL 60

Query: 63  GIGTLPDVGGTVNDIKANVDLNGNGGAVFDFTKHGAQGIHDNMDCS----------RNTV 122
           GIGTLPD+G TVNDI+AN+ LN NGG+VFD TKHGA+   +  D            RNTV
Sbjct: 61  GIGTLPDIGSTVNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTV 120

Query: 123 GPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFI 182
           GPAKF IPQGT+ VGPVTFAGPCKSFPITLENQGTVKATTDI++YSSPEWFSIEDI GFI
Sbjct: 121 GPAKFLIPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFI 180

Query: 183 LTGFGVFDGQGAAAWPYNNCKKNNF----------------------------------- 242
           LTG GVFDGQG   WPYN+CKKN F                                   
Sbjct: 181 LTGSGVFDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFY 240

Query: 243 ------------SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVT 302
                       +P NSPNTDGMHLSTSKLVTITNSVIG GDDCVSIGH  E + VTNVT
Sbjct: 241 CYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVT 300

Query: 303 CGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFEDVV 345
           CGPGH LSVGSLGKYSKEKS+Y+VLVKNC+IFN TNGARIKTWAS +SGLASGIIFED+V
Sbjct: 301 CGPGHGLSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIV 360

[Close]

BLAST of Lsi02G024220 vs. ExPASy TrEMBL
Match: A0A1S3BRD3 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 1.0e-130
Identity = 256/402 (63.68%), Postives = 284/402 (70.65%), Query Frame = 0


Query: 1   MTMTRSHSLFQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQP 60
           MT+TR   LFQILL +FA QCC KV+AI ND+   ANL   IVST+N QQL  P AAP P
Sbjct: 1   MTITRDIGLFQILLWVFAWQCCDKVSAITNDI---ANLNYEIVSTIN-QQLPSPVAAPGP 60

Query: 61  LGIGTLPDVGGTVN-DIKANVDLNGNGGAVFDFTKHGAQ--GIHDN--------MDCSRN 120
           LGI TLPD+   VN DI  N DLNGNGG+VF  TKHGA+  G  D+        ++  RN
Sbjct: 61  LGIETLPDLDNMVNDDINTNNDLNGNGGSVFHVTKHGAKADGKTDDAQAFVTTWIEACRN 120

Query: 121 TVGPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIG 180
           TVGPAK  IP+GT+ VGPVT AGPCKSFPITLENQGTVKATTDI+QYSSPEWFSIEDI G
Sbjct: 121 TVGPAKILIPEGTYLVGPVTLAGPCKSFPITLENQGTVKATTDISQYSSPEWFSIEDITG 180

Query: 181 FILTGFGVFDGQGAAAWPYNNCKKNN---------------------------------- 240
           FILTG GVFDGQG AAWPYN+CK NN                                  
Sbjct: 181 FILTGSGVFDGQGVAAWPYNDCKNNNICQLLPISIKFSRLNDTIVDRLTSLNSKGFHMSL 240

Query: 241 -------------FSPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTN 300
                         +P +SPNTDG+HLSTSKLV I NS+IG GDDCVSIGH  EK+TVTN
Sbjct: 241 FSCYNFTATNINIIAPHDSPNTDGIHLSTSKLVNIANSIIGTGDDCVSIGHSTEKITVTN 300

Query: 301 VTCGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFED 345
           VTCGPGH LSVGSLGKY +EK +YDVLVKNC+IFN TNGARIKT+AS +SG ASGIIFED
Sbjct: 301 VTCGPGHGLSVGSLGKYPEEKDVYDVLVKNCTIFNATNGARIKTFASPISGSASGIIFED 360

[Close]

BLAST of Lsi02G024220 vs. ExPASy TrEMBL
Match: A0A5D3C9J8 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00530 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 3.0e-130
Identity = 255/402 (63.43%), Postives = 283/402 (70.40%), Query Frame = 0


Query: 1   MTMTRSHSLFQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQP 60
           MT+TR   LFQILL +FA QCC KV+AI ND+   ANL   IVST+N QQL  P AAP P
Sbjct: 1   MTITRDIGLFQILLWVFAWQCCDKVSAITNDI---ANLNYEIVSTIN-QQLPSPVAAPGP 60

Query: 61  LGIGTLPDVGGTVN-DIKANVDLNGNGGAVFDFTKHGAQ--GIHDN--------MDCSRN 120
            GI TLPD+   VN DI  N DLNGNGG+VF  TKHGA+  G  D+        ++  RN
Sbjct: 61  FGIETLPDLDNMVNDDINTNNDLNGNGGSVFHVTKHGAKADGKTDDAQAFVTTWIEACRN 120

Query: 121 TVGPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIG 180
           TVGPAK  IP+GT+ VGPVT AGPCKSFPITLENQGTVKATTDI+QYSSPEWFSIEDI G
Sbjct: 121 TVGPAKILIPEGTYLVGPVTLAGPCKSFPITLENQGTVKATTDISQYSSPEWFSIEDITG 180

Query: 181 FILTGFGVFDGQGAAAWPYNNCKKNN---------------------------------- 240
           FILTG GVFDGQG AAWPYN+CK NN                                  
Sbjct: 181 FILTGSGVFDGQGVAAWPYNDCKNNNICQLLPISIKFSRLNDTIVDRLTSLNSKGFHMSL 240

Query: 241 -------------FSPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTN 300
                         +P +SPNTDG+HLSTSKLV I NS+IG GDDCVSIGH  EK+TVTN
Sbjct: 241 FSCYNFTATNINIIAPHDSPNTDGIHLSTSKLVNIANSIIGTGDDCVSIGHSTEKITVTN 300

Query: 301 VTCGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFED 345
           VTCGPGH LSVGSLGKY +EK +YDVLVKNC+IFN TNGARIKT+AS +SG ASGIIFED
Sbjct: 301 VTCGPGHGLSVGSLGKYPEEKDVYDVLVKNCTIFNATNGARIKTFASPISGSASGIIFED 360

[Close]

BLAST of Lsi02G024220 vs. ExPASy TrEMBL
Match: A0A6J1JX85 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111488306 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.2e-128
Identity = 246/403 (61.04%), Postives = 282/403 (69.98%), Query Frame = 0


Query: 1   MTMTRSHSLFQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQP 60
           MT TR+ SLFQ LLL+ ACQCC +VAA+F+ VTG ANL+DGIVST N Q   RP AAP P
Sbjct: 1   MTTTRNLSLFQTLLLVLACQCCAEVAAVFDHVTGTANLIDGIVSTANQQH--RPPAAPLP 60

Query: 61  LGIGTLPDVGGTV--NDIKANVDLNGNGGAVFDFTKHGAQGIHDNMDCS----------R 120
           LGI T  +  G+V  N  KA V L GNG AVFD  K+GA+    + D            R
Sbjct: 61  LGIRTPANRRGSVAINGTKAAVYLKGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACR 120

Query: 121 NTVGPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDII 180
           NT GPAKF IP+G F VGPVTFAGPCKS PIT+E QGTVKATTDI++YSSP+W SIE I 
Sbjct: 121 NTTGPAKFLIPKGIFLVGPVTFAGPCKSMPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GFILTGFGVFDGQGAAAWPYNNCKKNN--------------------------------- 240
           G ILTG GVFDGQGA+AWPYN+CK NN                                 
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKINNNCQILPNSIKFTRLNHSIVDGLTSINSKGFHTS 240

Query: 241 --------------FSPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVT 300
                          +PGNSPNTDGMHLSTSKLVTI++S IG GDDCVSIGH CEK+TVT
Sbjct: 241 VFRCYNFTATNMNIMAPGNSPNTDGMHLSTSKLVTISSSTIGTGDDCVSIGHSCEKITVT 300

Query: 301 NVTCGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFE 345
           NVTCGPGH +S+GSLG+Y  EKS+ DVLV+NC+IFN TNGARIKTWA+ +SG A GI+F+
Sbjct: 301 NVTCGPGHGISIGSLGRYKTEKSVLDVLVQNCTIFNATNGARIKTWANTISGSAVGIVFD 360

[Close]

BLAST of Lsi02G024220 vs. NCBI nr
Match: XP_038896336.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 555.4 bits (1430), Expect = 3.6e-154
Identity = 289/401 (72.07%), Postives = 311/401 (77.56%), Query Frame = 0


Query: 1   MTMTRSHSLFQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQP 60
           MTMT S SLFQILLL FA QCC +VAAIF+D+TG ANLV+G+VSTVN Q LL PAAAP P
Sbjct: 1   MTMT-SPSLFQILLLAFAWQCCAEVAAIFDDITGTANLVNGVVSTVN-QPLLHPAAAPLP 60

Query: 61  LGIGTLPDVGGTVNDIKANVDLNGNGGAVFDFTKHGAQGIHDNMDCS----------RNT 120
           LGIGTLPD+GGTVNDIKANVDLNGNGG+VFD TKHGA+G     D            RN 
Sbjct: 61  LGIGTLPDLGGTVNDIKANVDLNGNGGSVFDVTKHGAKGDGKTDDAQAFMTTWIEACRNA 120

Query: 121 VGPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGF 180
            GPAKF IPQGTF VGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIE+I GF
Sbjct: 121 EGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEEITGF 180

Query: 181 ILTGFGVFDGQGAAAWPYNNCKKN------------------------------------ 240
           ILTG GVFDGQGAA+WPYN+CKKN                                    
Sbjct: 181 ILTGSGVFDGQGAASWPYNDCKKNTKCQILPISIKFTKLNHTIVDGLASLNSKGFHTSIF 240

Query: 241 ---NF--------SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNV 300
              NF        +PGNSPNTDGMH+STSKLVTITNSVIG GDDCVSIGH  EK+ VTNV
Sbjct: 241 YCYNFTATNMNIIAPGNSPNTDGMHISTSKLVTITNSVIGTGDDCVSIGHSTEKIVVTNV 300

Query: 301 TCGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFEDV 345
           TCGPGH LSVGSLGKYSKEKS+YDVLVKNC+IFN TNGARIKTWAS +SGLASGI FED+
Sbjct: 301 TCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNATNGARIKTWASPISGLASGITFEDI 360

[Close]

BLAST of Lsi02G024220 vs. NCBI nr
Match: XP_038896337.1 (exopolygalacturonase clone GBGE184-like [Benincasa hispida])

HSP 1 Score: 526.2 bits (1354), Expect = 2.3e-145
Identity = 266/345 (77.10%), Postives = 286/345 (82.90%), Query Frame = 0


Query: 3   MTRSHSLFQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQPLG 62
           M +S SLFQILLL+ A QCC KVAAI +D TGA+NLVDGIVS VN Q+LL PAAAP+ LG
Sbjct: 1   MIKSLSLFQILLLVLAWQCCAKVAAILDDATGASNLVDGIVSMVN-QKLLHPAAAPRSLG 60

Query: 63  IGTLPDVGGTVNDIKANVDLNGNGGAVFDFTKHGAQGIHDNMDCS----------RNTVG 122
           IGTLPD+GGTVNDIKAN DLNGNGGAVFD TK+GA+      D            RNT G
Sbjct: 61  IGTLPDIGGTVNDIKANADLNGNGGAVFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDG 120

Query: 123 PAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFIL 182
           PAKF IPQGTF VGPVTFAGPCKSFPITLENQGTVKATTDIT+YSSPEWFSIEDI GFIL
Sbjct: 121 PAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITEYSSPEWFSIEDITGFIL 180

Query: 183 TGFGVFDGQGAAAWPYNNCKKNNF-------------SPGNSPNTDGMHLSTSKLVTITN 242
           TG GVFDGQG AAWPYN+CK NNF             +PGNSPNTDGMH+STSKLVTITN
Sbjct: 181 TGSGVFDGQGTAAWPYNDCKTNNFCQNLPIEKKKNIIAPGNSPNTDGMHISTSKLVTITN 240

Query: 243 SVIGIGDDCVSIGHGCEKVTVTNVTCGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVT 302
           SVIG GDDCVSIGH  EK+TVT VTCGPGH LSVGSLGKYSKEKS+YDVLVKNC+IFN T
Sbjct: 241 SVIGTGDDCVSIGHSTEKITVTGVTCGPGHGLSVGSLGKYSKEKSVYDVLVKNCTIFNAT 300

Query: 303 NGARIKTWASHMSGLASGIIFEDVVMYNVKNPIIIDQTHGTREKK 325
           NGARIKTWAS +SGLASGI FED+VMYNVKNPIIIDQT+GT+EKK
Sbjct: 301 NGARIKTWASPVSGLASGITFEDIVMYNVKNPIIIDQTYGTKEKK 344

[Close]

BLAST of Lsi02G024220 vs. NCBI nr
Match: KAA0048661.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >KAA0048665.1 exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK18163.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 517.3 bits (1331), Expect = 1.1e-142
Identity = 269/400 (67.25%), Postives = 295/400 (73.75%), Query Frame = 0


Query: 3   MTRSHSL-FQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQPL 62
           MTR+ SL  QILLL+FA QC  KVAA  +DVTG  NL+   V TVN +Q LRPA AP+ L
Sbjct: 1   MTRNLSLVIQILLLVFASQCSAKVAATLDDVTGTENLLKVTVPTVN-EQFLRPAGAPRRL 60

Query: 63  GIGTLPDVGGTVNDIKANVDLNGNGGAVFDFTKHGAQGIHDNMDCS----------RNTV 122
           GIGTLPD+G TVNDI+AN+ LN NGG+VFD TKHGA+   +  D            RNTV
Sbjct: 61  GIGTLPDIGSTVNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTV 120

Query: 123 GPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFI 182
           GPAKF IPQGT+ VGPVTFAGPCKSFPITLENQGTVKATTDI++YSSPEWFSIEDI GFI
Sbjct: 121 GPAKFLIPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFI 180

Query: 183 LTGFGVFDGQGAAAWPYNNCKKNNF----------------------------------- 242
           LTG GVFDGQG   WPYN+CKKN F                                   
Sbjct: 181 LTGSGVFDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFY 240

Query: 243 ------------SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVT 302
                       +P NSPNTDGMHLSTSKLVTITNSVIG GDDCVSIGH  E + VTNVT
Sbjct: 241 CYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVT 300

Query: 303 CGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFEDVV 345
           CGPGH LSVGSLGKYSKEKS+Y+VLVKNC+IFN TNGARIKTWAS +SGLASGIIFED+V
Sbjct: 301 CGPGHGLSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIV 360

[Close]

BLAST of Lsi02G024220 vs. NCBI nr
Match: KAA0043575.1 (exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 514.6 bits (1324), Expect = 7.0e-142
Identity = 268/400 (67.00%), Postives = 294/400 (73.50%), Query Frame = 0


Query: 3   MTRSHSL-FQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQPL 62
           MTR+ SL  QILLL+FA QC  K AA  +DVTG  NL+   V TVN +Q LRPA AP+ L
Sbjct: 1   MTRNLSLVIQILLLVFASQCSAKDAATLDDVTGTENLLKVTVPTVN-EQFLRPAGAPRRL 60

Query: 63  GIGTLPDVGGTVNDIKANVDLNGNGGAVFDFTKHGAQGIHDNMDCS----------RNTV 122
           GIGTLPD+G TVNDI+AN+ LN NGG+VFD TKHGA+   +  D            RNTV
Sbjct: 61  GIGTLPDIGSTVNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTV 120

Query: 123 GPAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFI 182
           GPAKF IPQGT+ VGPVTFAGPCKSFPITLENQGTVKATTDI++YSSPEWFSIEDI GFI
Sbjct: 121 GPAKFLIPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFI 180

Query: 183 LTGFGVFDGQGAAAWPYNNCKKNNF----------------------------------- 242
           LTG GVFDGQG   WPYN+CKKN F                                   
Sbjct: 181 LTGSGVFDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFY 240

Query: 243 ------------SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVT 302
                       +P NSPNTDGMHLSTSKLVTITNSVIG GDDCVSIGH  E + VTNVT
Sbjct: 241 CYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVT 300

Query: 303 CGPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFEDVV 345
           CGPGH LSVGSLGKYSKEKS+Y+VLVKNC+IFN TNGARIKTWAS +SGLASGIIFED+V
Sbjct: 301 CGPGHGLSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIV 360

[Close]

BLAST of Lsi02G024220 vs. NCBI nr
Match: XP_031745094.1 (exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 513.8 bits (1322), Expect = 1.2e-141
Identity = 266/399 (66.67%), Postives = 293/399 (73.43%), Query Frame = 0


Query: 3   MTRSHSLFQILLLIFACQCCGKVAAIFNDVTGAANLVDGIVSTVNDQQLLRPAAAPQPLG 62
           MTR+ SL QILLL+FA QCC   A +F+D+TG  N + GIV T N +Q LRPA AP+ LG
Sbjct: 1   MTRNLSLIQILLLVFAWQCC---AVVFDDITGTQNRLKGIVPTTN-EQFLRPAEAPRRLG 60

Query: 63  IGTLPDVGGTVNDIKANVDLNGNGGAVFDFTKHGAQGIHDNMDCS----------RNTVG 122
             TLP++GGTVNDIKANVDLN NGG+VFD TKHGA+      D            RNTVG
Sbjct: 61  FSTLPNIGGTVNDIKANVDLNQNGGSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVG 120

Query: 123 PAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFIL 182
           PAKF IPQGTF VGPVTFAGPCKSFPITLENQGTVKATTDI+ YSSPEWFSIEDI GFIL
Sbjct: 121 PAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFIL 180

Query: 183 TGFGVFDGQGAAAWPYNNCKKNN------------------------------------- 242
           TG GVFDGQG + WPYN+CKKNN                                     
Sbjct: 181 TGSGVFDGQGLSVWPYNDCKKNNLCQLLPISIKFTRLNHTIVDGLTSVNSMGFHTSVFYC 240

Query: 243 ----------FSPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTC 302
                      +P NSPNTDGMHLSTSKLVTI NSVIG GDDCVSIGH  E +TVTNVTC
Sbjct: 241 YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTENITVTNVTC 300

Query: 303 GPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFEDVVM 345
           GPGH LSVGSLGKYSKEK +YDVLVKNC+IFN TNGARIKTWAS +SGLAS IIFED+VM
Sbjct: 301 GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPVSGLASRIIFEDIVM 360

[Close]

BLAST of Lsi02G024220 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 216.9 bits (551), Expect = 2.8e-56
Identity = 132/323 (40.87%), Postives = 163/323 (50.46%), Query Frame = 0


Query: 83  NGNGGAVFDFTKHGAQGIHDNMDCSRNTVG--------------PAKFFIPQGTFSVGPV 142
           N     V+D TK GA G     D S NT                PA   +P+GTF  GPV
Sbjct: 45  NSGNPTVYDITKFGAVG-----DGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPV 104

Query: 143 TFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFILTGFGVFDGQGAAAWPY 202
            FAGPCKS  +T+   GT+ ATT  + Y++PEWF  E +   +LTG G F G+G A W  
Sbjct: 105 IFAGPCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKA 164

Query: 203 NNCKK---------------------NNFS--------------------------PGNS 262
           + C K                     N  S                          P  S
Sbjct: 165 DGCGKKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAES 224

Query: 263 PNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTCGPGHDLSVGSLGKYSK 322
           PNTDG+HLS +  V+I +S I  GDDCVS+G G   VTV  V CGPGH LSVGSLGKY  
Sbjct: 225 PNTDGIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKN 284

Query: 323 EKSIYDVLVKNCSIFNVTNGARIKTWASHMSGLASGIIFEDVVMYNVKNPIIIDQTHGTR 345
           E+ +  + V NC++    NG RIKTW       A  I FE+++M +VKNPIIIDQ +G+R
Sbjct: 285 EEDVSGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSR 344

[Close]

BLAST of Lsi02G024220 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 199.1 bits (505), Expect = 6.0e-51
Identity = 118/319 (36.99%), Postives = 165/319 (51.72%), Query Frame = 0


Query: 80  VDLNGNGGAVFDFTKHGAQGIHDNMDCSRNTVGPAKFFIPQGTFSVGPVTFAGPCKSFP- 139
           VD+   G    D   H    +       +++       IP+G FSVG + F+GPC +   
Sbjct: 35  VDVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGPCTNVSN 94

Query: 140 ITLENQGTVKATTDITQY-SSPEWFSIEDIIGFILTGFGVFDGQGAAAWPYNNC------ 199
           +T+     VKA+TD+++Y S   W     I G  LTG G FDGQGA AWP+NNC      
Sbjct: 95  LTVR----VKASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNCTSDSNC 154

Query: 200 -----------------------------------------KKNNFSPGNSPNTDGMHLS 259
                                                    + N  +P +SPNTDG+H+ 
Sbjct: 155 KLLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIE 214

Query: 260 TSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTCGPGHDLSVGSLGKYSKEKSIYDVLV 319
            S  V  + S I  GDDCVSIG G  ++T+T++ CGPGH +SVGSLG+Y  EK +  ++V
Sbjct: 215 RSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVV 274

Query: 320 KNCSIFNVTNGARIKTWASHMS-GLASGIIFEDVVMYNVKNPIIIDQTHGTREKKASN-- 345
           K+C I   TNG RIKTWA+      A+ + FE+++M NV NPIIIDQ++       SN  
Sbjct: 275 KDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNVP 334

[Close]

BLAST of Lsi02G024220 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 194.5 bits (493), Expect = 1.5e-49
Identity = 115/282 (40.78%), Postives = 150/282 (53.19%), Query Frame = 0


Query: 113 PAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFIL 172
           P+K  IP+G F +G +   GPCK+ PI +  QGTVKA  +  Q    +W    +I GF L
Sbjct: 52  PSKVVIPKGEFKLGEIEMRGPCKA-PIEVTLQGTVKADGNAIQ-GKEKWVVFGNIDGFKL 111

Query: 173 TGFGVFDGQGAAAWPYNNCKK--------------------------------------- 232
            G G FDG+G AAW  NNC K                                       
Sbjct: 112 NGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIRDISSIDAKNFHINVLGA 171

Query: 233 -----NNF---SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTC 292
                NN    +P +SPNTDG+HL  S  V I NS I  GDDC+S+G G + + V  VTC
Sbjct: 172 KNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDCISVGDGMKNLHVEKVTC 231

Query: 293 GPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWAS-HMSGLASGIIFEDVV 343
           GPGH +SVGSLG+Y  E+ +  + V NC++    NG RIKTW S   S  AS I FED++
Sbjct: 232 GPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACSTTASDIHFEDII 291

[Close]

BLAST of Lsi02G024220 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 189.1 bits (479), Expect = 6.2e-48
Identity = 111/281 (39.50%), Postives = 146/281 (51.96%), Query Frame = 0


Query: 114 AKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFILT 173
           +K  I +G F +G +   GPCK+ P+ +  QGT+KA     Q     W     I GF L 
Sbjct: 54  SKVVITKGEFKLGEIEMTGPCKA-PVEINLQGTLKADGKAIQ-GKERWVVFLRINGFKLN 113

Query: 174 GFGVFDGQGAAAWPYNNCKK-----------------------------NNF-------- 233
           G G+FDG+G AAW  NNC K                              NF        
Sbjct: 114 GGGIFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAK 173

Query: 234 ----------SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTCG 293
                     +P  SPNTDG+HL  S+ V I NS I  GDDC+S+G G + + V NV CG
Sbjct: 174 NMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCG 233

Query: 294 PGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWAS-HMSGLASGIIFEDVVM 343
           PGH +SVGSLG+Y  E+ +  + V NC++    NG RIKTW S   +  ASGI FE++++
Sbjct: 234 PGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIIL 293

[Close]

BLAST of Lsi02G024220 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 6.9e-47
Identity = 109/282 (38.65%), Postives = 149/282 (52.84%), Query Frame = 0


Query: 113 PAKFFIPQGTFSVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEDIIGFIL 172
           P K  IP+G F +G +  +GPCKS P+ +   GTV A  + + +   +W   + + GF L
Sbjct: 53  PRKVVIPKGQFKLGEIMMSGPCKS-PVEITLLGTVLADGN-SIHGKEKWVVFQRMDGFRL 112

Query: 173 TGFGVFDGQGAAAWPYNNCKK-----------------------------NNF------- 232
            G G FDG+G AAW  NNC K                              NF       
Sbjct: 113 NGGGTFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVTNAEIRDISSIDAKNFHINVIGA 172

Query: 233 -----------SPGNSPNTDGMHLSTSKLVTITNSVIGIGDDCVSIGHGCEKVTVTNVTC 292
                      +P  SPNTDG+HL  S  V+I NS I  GDDCVS+G G   + V NV C
Sbjct: 173 KNMTFDNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDCVSVGDGMVNLLVKNVVC 232

Query: 293 GPGHDLSVGSLGKYSKEKSIYDVLVKNCSIFNVTNGARIKTWAS-HMSGLASGIIFEDVV 343
           GPGH +SVGSLG+Y  E+ +  + V NC++    NG RIKTW S   S  AS I FE+++
Sbjct: 233 GPGHGISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWPSAACSTTASNIHFENII 292

The following BLAST results are available for this feature:

BLAST of Lsi02G024220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49062	3.9e-55	40.87	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ...	[more]
Q6H9K0	2.5e-54	36.91	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
Q05967	2.2e-53	38.26	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]
P35339	9.1e-52	38.14	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]
Q39766	4.2e-49	36.27	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]

BLAST of Lsi02G024220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U4W1	5.2e-143	67.25	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7TQ89	3.4e-142	67.00	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A1S3BRD3	1.0e-130	63.68	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1	[more]
A0A5D3C9J8	3.0e-130	63.43	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A6J1JX85	1.2e-128	61.04	exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111488306 PE=3 SV=1	[more]

BLAST of Lsi02G024220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896336.1	3.6e-154	72.07	exopolygalacturonase-like [Benincasa hispida]	[more]
XP_038896337.1	2.3e-145	77.10	exopolygalacturonase clone GBGE184-like [Benincasa hispida]	[more]
KAA0048661.1	1.1e-142	67.25	exopolygalacturonase-like [Cucumis melo var. makuwa] >KAA0048665.1 exopolygalact...	[more]
KAA0043575.1	7.0e-142	67.00	exopolygalacturonase-like [Cucumis melo var. makuwa]	[more]
XP_031745094.1	1.2e-141	66.67	exopolygalacturonase-like [Cucumis sativus]	[more]

BLAST of Lsi02G024220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02790.1	2.8e-56	40.87	polygalacturonase 4	[more]
AT4G18180.1	6.0e-51	36.99	Pectin lyase-like superfamily protein	[more]
AT3G07820.1	1.5e-49	40.78	Pectin lyase-like superfamily protein	[more]
AT3G07840.1	6.2e-48	39.50	Pectin lyase-like superfamily protein	[more]
AT3G07830.1	6.9e-47	38.65	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 265..286 e-value: 470.0 score: 9.5 coord: 325..360 e-value: 2200.0 score: 4.8 coord: 295..316 e-value: 1700.0 score: 5.6 coord: 212..233 e-value: 1400.0 score: 6.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 87..195 e-value: 2.7E-12 score: 48.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 196..363 e-value: 6.3E-51 score: 175.2
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 198..345 e-value: 8.9E-46 score: 156.4 coord: 111..195 e-value: 2.5E-10 score: 39.9
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 2..195 coord: 196..355
None	No IPR available	PANTHER	PTHR31375:SF158	POLYGALACTURONASE	coord: 2..195 coord: 196..355
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..19 score: 5.0
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 89..355

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G024220.1	Lsi02G024220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity