Lsi02G023760 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G023760
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	wee1-like protein kinase
Location	chr02: 30457147 .. 30461881 (-)
RNA-Seq Expression	Lsi02G023760
Synteny	Lsi02G023760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGCGTGGGAGGGAAAGCTTCACCATAGATTCTTTTGATGAGGAGAAAATCCAAAACCCTAAGAACGACGATGACGACGAGGAGGAAGAAGATGAAAGGGACATTGACGAGGCACTTGCAGGTTCAGCTAGGGCAAGTTTCACTCGCTCCTCTTCACCTCCACCGCTCCTCTTCCGCGGCCGCCGCTTCCTCCGCCGTTTGCTTCCAGGACTTGCCTGAATCTGAAGCACCGCCGATGGTCAACGCTTCCGACGCTCTCCCCTCCTCCTCTCCTAACTTTGAGGACAGAGATTTCATTCTTAGCCAGGACTTCTTCTGGTGAGTTTTGTATCGTTATTTTTCTTTTCATTTATACCATCTGTGAGTTGGTTTGAGCTGCTGTTGATGGATTTGGAGGGAATAGTTGGTTGATCTTTGTTATCTTTCTCGACGTGCTGTGAGATGGAGGACTTTTAAATAATGTATTTTTTTTTTATCACTTTTGCTTATGAGATAGTTAATTTTAGTTTTCGTTTATGGAAGCCATTTCTTAGTTACTTAAGTAGAGTTTAAGTGTTCTAAACCCTAAATCCTAAACTTAAATAGACCGAACAATGTAGTATGAACTACTAATTATTGAATACTTTTAGAAATTACTTTTGCTTGTCAATATTGAGATTGTATACATGGATGCTAAATGGTAGCTGGTGAGATTGAAGGCTGCAGTGTAACTGACCAGATAATTATTGGGTTGTTTGTTGCAGTACCCCTGACTATATCACCCCGGACAATCAAAATTTGTTGAATGGTGTGGATAGCAACAAGGTGAGCAGTTAATGGAGAAAGCTAACCAGGGCAAACACAAGGGTTTCTTTTCAGAAGAAAAAGCTAACTCTCTAATTTTTCTGGGTGTCCTTGTCTTGGCAGGAATGCATTCCTTGCCCCAAATCTCCAGAGAAGATTAATACTGTTAAAACTAAGAGAAAAATACATGGTAAGTTGACTATTTTGAACTTTTATCTCAGTTAATTAACTGTATTGAGTATGACTGGTATAAGAGATCAGATGCATGCAAATGGCACACACTTAATCTAAGAAACACAAATATGGATACGACATGACATGAACACTGGACATGTCATTTCTTAATAGTCTAGATATAGTAAGGACACATTTATTTTAATGTACAGTTTTAAAAATATATTTCATTTTTTTACCAGAAGAAAATTCGAAGTAGATGGGTTGATGCTAAATCATTTATATACTTAAAAAATTAGTTTGATGTATTTCACGCTCAAAATTATTTTCACATATGTGTCTTTTTAGTGTACACAACAAGTGTTCTATGCATGTCTAACACATTTGTTGTACTAACAAGTGTCCGCTACGTGTCCAAATGCCCGACACTTATTGGACATAGACATGCTAGCCAAACTAAACTGCCTATGCTTCTTAACACGTAACTGTAATCATGTTGAAGCTGAACTTTCCAAATCAATAATGTAAATGGTTAATCACTTGCAACTAATGATGATTATTGATTAGTGTCGGAGTCATTTATCAATATGGGGACATTGATCTGAAGTATTTTTGGGGCTTCAAAATCTGACAGCCTTGTATTCCTTTTTGAAATTAGTGATGTAAGTTGTTCGAATGATGTAATGTAATCATTTTCTTCATTAATATTCATTTTGGTGCAGAAAGTGTTTTAGTACATCCTCTGAGTCCGAGTTTGTCTGGCTGCGAACAAGTTGTGGAGCTTGGAAATGACATGTTTGGATCAGATGATGTGGATATGGAGAAAGAGACAATGGCCATGCCAAAGAAAGAAAACTATGTTTCTCAATCTGCAGTTGCTCTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAACCCATACTTGAAAGATGCTTCAGATGTAGGCGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGTACAGTTCAATTAGTTCTTAAAGACATCAAAAGTAATTATACAATTTTTGAAATCGTCTTTTGCAATGATGATGGAGTTCTGTTAAATGAACTAGTTTCGTGGTTTTCAATTTTGTGTAGGATTTTTTCCTGCGCTGTTTAGCGGAGATGGTCTTTCAAGATATCACACTGATTTTCATGAGATAAAGGTATCCAGAAACATCTGCTTAGTGCCTACAAGCTTCTGCTGATAGAGATTGTGATTTTTTTTTAAATTCTTATTTGATTCAGGCTGTCAAACTTTGCAATAACTTCAGTCTATTTTCAGCAAATAGGGACTGGAAATTTTAGCCGTGTCTTCAAGGTATTGAAGAGAATTGATGGTTGCTTATATGCTGTCAAACAAAGCACAAGACCGTTGAATCAAGACGTAGAAAGGTTACTTCTTTTGGTGTGTGTCTGTATATGTTTTACCTCTAAACACTAGCAAATGTGATTGAATTTTTTCCTTCCCTCTGCAGGAGGAGAGCGTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGTTAGTAAATTTATCTAGATATGGTTTTCCGTTCTATTATATACATAGATAAATATGTTCCTTCTTCTCTCTAGAGCAATCTTTTTTTAAATTTTTTTCTTGATTGTTGTTCTCTTTTGAACGTGTAGGATCTCATGAAAACATTGTTGGATACTACTCTTCATGGTTTGAAAATGAACAACTCTATATTCAAATGGAGCTCTGTGATTTTAGTTTATCAATTGGCAGGTATTCCCACCCATTCTCTGAGGTAGATGCGCTGAGAGCCTTGTATCAGGTGAGTTTTAGTTCCATCTTGAATTGTGAAACATTACATTTTCTGCTTACGAAACTTCATTTCCATAAATATTTCTTTCGTTAACACATAAAAGAAAGCTTAAGTTTGATTTGTTACTTGGAAGAGTGAATTTGTTTTTTCTGCGCTTCATTCAATACGTTAATTTTAGCAGGAAATGCCTCGTCTGAGAAATACTGATCCTAATTGTTATAAATTTTGTATTAATGTTTTATCTTTATCCATTACAATTCTTATGTAGCCTTAGTATTTAACATAGAAGTTTTATTCAATCCAATTTTCAAGTTCTAGAACTGGTATTATGAAATTTCATGGCTTCTATGCTGACGATGGAACTACAAACAGATTGCAAAGGCATTGCTATTTGTGCACGAGAAAGGAGTAGCTCATTTAGATGTGAAACCTGACAATATTTATGTCAAAGATGGTGTCTATAAACTTGGTGATTTTGGGTGTGTTACCCTTCTTGATAAGAGCTTACCGATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAAAGATATGATTATCTTGACAAAGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAGATTGCCAGAGGATCCACATTACCAGAAACGCATTTCATGAACCTTAAGGAGGGAAAATTGCCACTCCTTCCTGGTCATTCATTGCAATTTCAAAACTTAATCAAGGTACTGATTACTAGTGTCTTATTTTGGTTCTCTTCATTTCCTCTCACTATGCTTTTTGGCCTGATCTTTAGGCCTTGACTCACCTTCTCACCCTCCCCTCTTTGGATGATGAACCTTGTGGTGGAATCTGGTCATTGTATGTTTGCACATGTTTGGATTTCGTCCCACTAATTTTACTTTCCATTAAAAAAATGTAAAAAAGTTTGATTGTCAGAAGGTCAAAATTTATGGAAAAGTAAATCTGAACAAAGTTGCATCTCTACCTTCCTAAAGATAAAAGGAAGCTGCATTTATTTTTTCCTTAGAAAGTATCCCCAGATTATGTTAACCTATGTTTGCCAAAAGGAATATTTGATTTTGTTTGTTTGAACCGTCTGAGTATAAAATGTAGTGGTCTTTTAGAAAAGATGAATGAGATAAATAAGACCAGACATATCTTTTTGAGAATGAACTAATTTTAGATATTCTCTTAGCTATCTATTCTACGTTTGTATAGTTTGAGCTGATTGCTTTCCTACATTCTTTTATGGGTAGAAATGTTGTTTGAGAAGCCTGATTTTAGCATTCCGGCAACAAAAGTCATCAAACAAAAATCCCCAACAAGATGTATTTTCTAAGTTTCTCTTTATTTGATAAACAAAAGTTCATCACAAAACTAATTGACAATAAGAAGACTAATCTATCCACTTTATAAGAAGTGCGAGGTCTCCATCTTTTCAATGTGGTTACCTCAATATGCCCCCTCATTATTGTTCTTCTTTGAGTTCACCATTCTTGAATTGATTATTTTTTTTTGAACCAAATATCTTTTATATGATCAAGAAGAGAAATTAGTGAAGAAACTTATCGCTGATAAAAGCATGGATTTTAAGATTAACTTTGCATAACGATAAGGAAATGTTCACTATTTACTTCATTATCTTGTGACCTTGCAATTAACAGGCTATGGTGGATCCCGACCCGACACGGCGGCCTTCGGCAAGGGAAGTTCTTGAGAATCGCATATTTGATAAGGTCAGAAATCATAAACAATCATAGGACAATGTCAACTATGGCGTCTTCTTATTGTTTCACCATTTTCACACACGGGCTTTGCTTGTGCAATCCCTTGATATCAGCTTGCTATTTTGTAAGATCATTCTCCACTTTCCCAAAGTTCATGCACCAAATTTTGCCTATAGCATGGTGCAAGTTTTGTAAATTCTCTATCATGTATTATAAACTCGTAAATGCAAGTTAATGAAAGCTCTTTCATTTTCAAA

mRNA sequence

GAGCGTGGGAGGGAAAGCTTCACCATAGATTCTTTTGATGAGGAGAAAATCCAAAACCCTAAGAACGACGATGACGACGAGGAGGAAGAAGATGAAAGGGACATTGACGAGGCACTTGCAGGTTCAGCTAGGGCAAGTTTCACTCGCTCCTCTTCACCTCCACCGCTCCTCTTCCGCGGCCGCCGCTTCCTCCGCCGTTTGCTTCCAGGACTTGCCTGAATCTGAAGCACCGCCGATGGTCAACGCTTCCGACGCTCTCCCCTCCTCCTCTCCTAACTTTGAGGACAGAGATTTCATTCTTAGCCAGGACTTCTTCTGTACCCCTGACTATATCACCCCGGACAATCAAAATTTGTTGAATGGTGTGGATAGCAACAAGGAATGCATTCCTTGCCCCAAATCTCCAGAGAAGATTAATACTGTTAAAACTAAGAGAAAAATACATGAAAGTGTTTTAGTACATCCTCTGAGTCCGAGTTTGTCTGGCTGCGAACAAGTTGTGGAGCTTGGAAATGACATGTTTGGATCAGATGATGTGGATATGGAGAAAGAGACAATGGCCATGCCAAAGAAAGAAAACTATGTTTCTCAATCTGCAGTTGCTCTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAACCCATACTTGAAAGATGCTTCAGATGTAGGCGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGATTTTTTCCTGCGCTGTTTAGCGGAGATGGTCTTTCAAGATATCACACTGATTTTCATGAGATAAAGCAAATAGGGACTGGAAATTTTAGCCGTGTCTTCAAGGTATTGAAGAGAATTGATGGTTGCTTATATGCTGTCAAACAAAGCACAAGACCGTTGAATCAAGACGTAGAAAGGAGGAGAGCGTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGATCTCATGAAAACATTGTTGGATACTACTCTTCATGGTTTGAAAATGAACAACTCTATATTCAAATGGAGCTCTGTGATTTTAGTTTATCAATTGGCAGGTATTCCCACCCATTCTCTGAGGTAGATGCGCTGAGAGCCTTGTATCAGATTGCAAAGGCATTGCTATTTGTGCACGAGAAAGGAGTAGCTCATTTAGATGTGAAACCTGACAATATTTATGTCAAAGATGGTGTCTATAAACTTGGTGATTTTGGGTGTGTTACCCTTCTTGATAAGAGCTTACCGATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAAAGATATGATTATCTTGACAAAGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAGATTGCCAGAGGATCCACATTACCAGAAACGCATTTCATGAACCTTAAGGAGGGAAAATTGCCACTCCTTCCTGGTCATTCATTGCAATTTCAAAACTTAATCAAGGCTATGGTGGATCCCGACCCGACACGGCGGCCTTCGGCAAGGGAAGTTCTTGAGAATCGCATATTTGATAAGGTCAGAAATCATAAACAATCATAGGACAATGTCAACTATGGCGTCTTCTTATTGTTTCACCATTTTCACACACGGGCTTTGCTTGTGCAATCCCTTGATATCAGCTTGCTATTTTGTAAGATCATTCTCCACTTTCCCAAAGTTCATGCACCAAATTTTGCCTATAGCATGGTGCAAGTTTTGTAAATTCTCTATCATGTATTATAAACTCGTAAATGCAAGTTAATGAAAGCTCTTTCATTTTCAAA

Coding sequence (CDS)

ATGAGGAGAAAATCCAAAACCCTAAGAACGACGATGACGACGAGGAGGAAGAAGATGAAAGGGACATTGACGAGGCACTTGCAGGTTCAGCTAGGGCAAGTTTCACTCGCTCCTCTTCACCTCCACCGCTCCTCTTCCGCGGCCGCCGCTTCCTCCGCCGTTTGCTTCCAGGACTTGCCTGAATCTGAAGCACCGCCGATGGTCAACGCTTCCGACGCTCTCCCCTCCTCCTCTCCTAACTTTGAGGACAGAGATTTCATTCTTAGCCAGGACTTCTTCTGTACCCCTGACTATATCACCCCGGACAATCAAAATTTGTTGAATGGTGTGGATAGCAACAAGGAATGCATTCCTTGCCCCAAATCTCCAGAGAAGATTAATACTGTTAAAACTAAGAGAAAAATACATGAAAGTGTTTTAGTACATCCTCTGAGTCCGAGTTTGTCTGGCTGCGAACAAGTTGTGGAGCTTGGAAATGACATGTTTGGATCAGATGATGTGGATATGGAGAAAGAGACAATGGCCATGCCAAAGAAAGAAAACTATGTTTCTCAATCTGCAGTTGCTCTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAACCCATACTTGAAAGATGCTTCAGATGTAGGCGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGATTTTTTCCTGCGCTGTTTAGCGGAGATGGTCTTTCAAGATATCACACTGATTTTCATGAGATAAAGCAAATAGGGACTGGAAATTTTAGCCGTGTCTTCAAGGTATTGAAGAGAATTGATGGTTGCTTATATGCTGTCAAACAAAGCACAAGACCGTTGAATCAAGACGTAGAAAGGAGGAGAGCGTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGATCTCATGAAAACATTGTTGGATACTACTCTTCATGGTTTGAAAATGAACAACTCTATATTCAAATGGAGCTCTGTGATTTTAGTTTATCAATTGGCAGGTATTCCCACCCATTCTCTGAGGTAGATGCGCTGAGAGCCTTGTATCAGATTGCAAAGGCATTGCTATTTGTGCACGAGAAAGGAGTAGCTCATTTAGATGTGAAACCTGACAATATTTATGTCAAAGATGGTGTCTATAAACTTGGTGATTTTGGGTGTGTTACCCTTCTTGATAAGAGCTTACCGATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAAAGATATGATTATCTTGACAAAGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAGATTGCCAGAGGATCCACATTACCAGAAACGCATTTCATGAACCTTAAGGAGGGAAAATTGCCACTCCTTCCTGGTCATTCATTGCAATTTCAAAACTTAATCAAGGCTATGGTGGATCCCGACCCGACACGGCGGCCTTCGGCAAGGGAAGTTCTTGAGAATCGCATATTTGATAAGGTCAGAAATCATAAACAATCATAG

Protein sequence

MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLPESEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKENYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHENIVGYYSSWFENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENRIFDKVRNHKQS

Homology

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BLAST of Lsi02G023760 vs. ExPASy Swiss-Prot
Match: Q8L4H0 (Wee1-like protein kinase OS=Arabidopsis thaliana OX=3702 GN=WEE1 PE=1 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 5.5e-161
Identity = 294/492 (59.76%), Postives = 369/492 (75.00%), Query Frame = 0


Query: 2   RRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLPE 61
           R+   T++T  + + +KM+GTL RH  +Q GQ+S        SS+ A+++    FQ L +
Sbjct: 13  RKTQGTIKTRASKKIRKMEGTLERHSLLQFGQLSKISFENRPSSNVASSA----FQGLLD 72

Query: 62  SEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPK 121
           S++  + N   +  S + N  ++DFILSQDFFCTPDYITPDNQNL++G+D +K+  PCP+
Sbjct: 73  SDSSELRNQLGSADSDA-NCGEKDFILSQDFFCTPDYITPDNQNLMSGLDISKDHSPCPR 132

Query: 122 SPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKEN 181
           SP K+NTVK+KR   ES   +  + + S   +V E  ND   +D+V  +K      ++  
Sbjct: 133 SPVKLNTVKSKRCRQESFTGNHSNSTWSSKHRVDEQENDDIDTDEVMGDKLQANQTERTG 192

Query: 182 YVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHT 241
           YVSQ+AVALRCR MPPPC+KNPY+ + S+   DPFG+QR+KCA F P   SGDGLSRY T
Sbjct: 193 YVSQAAVALRCRAMPPPCLKNPYVLNQSETATDPFGHQRSKCASFLPVSTSGDGLSRYLT 252

Query: 242 DFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHE 301
           DFHEI+QIG G+FSRVFKVLKR+DGCLYAVK STR L  D ERR+A+MEVQALAALG HE
Sbjct: 253 DFHEIRQIGAGHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQALAALGFHE 312

Query: 302 NIVGYYSSWFENEQLYIQMELCDFSLSI--GRYSHPFSEVDALRALYQIAKALLFVHEKG 361
           NIVGYYSSWFENEQLYIQ+ELCD SLS    + S   SE + L  ++QIAKAL FVHEKG
Sbjct: 313 NIVGYYSSWFENEQLYIQLELCDHSLSALPKKSSLKVSEREILVIMHQIAKALHFVHEKG 372

Query: 362 VAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDI 421
           +AHLDVKPDNIY+K+GV KLGDFGC T LDKSLP+EEGDARYMPQEILNE Y++LDKVDI
Sbjct: 373 IAHLDVKPDNIYIKNGVCKLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYEHLDKVDI 432

Query: 422 FSLGAAIYEIARGSTLPET--HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAR 481
           FSLG  +YE+ +GS L E+    +N+KEGKLPLLPGHSLQ Q L+K M+D DP RRPSAR
Sbjct: 433 FSLGVTVYELIKGSPLTESRNQSLNIKEGKLPLLPGHSLQLQQLLKTMMDRDPKRRPSAR 492

Query: 482 EVLENRIFDKVR 490
           E+L++ +FD++R
Sbjct: 493 ELLDHPMFDRIR 499

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BLAST of Lsi02G023760 vs. ExPASy Swiss-Prot
Match: Q6Z829 (Wee1-like protein kinase OS=Oryza sativa subsp. japonica OX=39947 GN=WEE1 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.5e-137
Identity = 268/481 (55.72%), Postives = 339/481 (70.48%), Query Frame = 0


Query: 27  LQVQLGQVSLAPL--HLHRSSSAAAASSAVCFQDLPESEAPPMVNASDALPSSSPN---- 86
           L +QL  VSL        R  +AAA +    F++L E    P       LP   P     
Sbjct: 42  LSLQLEHVSLFSFLADAPREGAAAARTPFTPFEELLEGSCDPDPTPPPPLPPLQPQATPM 101

Query: 87  -----FEDRDF-ILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKTKRK 146
                 E++D  ILSQDFFCTPDYITPD   L +G D+NKE IPCP SPEK +  ++KR 
Sbjct: 102 DADEVVEEKDSGILSQDFFCTPDYITPDAPQLGSGFDANKENIPCPNSPEK-SVCRSKRY 161

Query: 147 IHESVLVHPLSPSLSGCEQVVE---LGNDMF-GSDDVDMEK-ETMAMPKKENYVSQSAVA 206
             +       SP   G   + +   +    F G DD + E+ +  +  K+ +YVSQSAVA
Sbjct: 162 KRD------CSPKGLGSNDIFDSQWIAPVQFEGLDDSEEEQLKESSSHKRGSYVSQSAVA 221

Query: 207 LRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKQI 266
           LRCRVMPPPC++NPYL     +  + FG ++ K +GF P++  GDGLSRY TDFHEI+QI
Sbjct: 222 LRCRVMPPPCIRNPYLNTDHQIDDNVFGGRQCKSSGFSPSV-DGDGLSRYRTDFHEIEQI 281

Query: 267 GTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHENIVGYYSS 326
           G GNFS VFKVLKRIDGCLYAVK+S R L+ D ERR+A+ EVQALAALG HENIVGY++S
Sbjct: 282 GRGNFSVVFKVLKRIDGCLYAVKRSIRQLHNDRERRQAVKEVQALAALGCHENIVGYFTS 341

Query: 327 WFENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHLDVKPDN 386
           WFEN+QL+IQMELCD  LS+ R + P    +AL  LYQI K L F+HE+G+AHLDVKPDN
Sbjct: 342 WFENKQLFIQMELCDRCLSMDR-NQPLKCGEALELLYQICKGLDFIHERGIAHLDVKPDN 401

Query: 387 IYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI 446
           IYV++GVYKLGDFGC TL+D+SL IE+GD+RYMP E+LN++Y++LDKVDIFSLGAAIYE+
Sbjct: 402 IYVRNGVYKLGDFGCATLIDRSLAIEDGDSRYMPPEMLNDKYEHLDKVDIFSLGAAIYEL 461

Query: 447 ARGSTLPET--HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENRIFDK 489
            RG+ LP++   F +L+EGK+ LLPG  +QFQ+LIK+M+DPDP RRPSA+EVL + IFDK
Sbjct: 462 IRGTQLPDSGPQFTSLREGKIALLPGCPMQFQSLIKSMMDPDPVRRPSAKEVLRHPIFDK 513

[Close]

BLAST of Lsi02G023760 vs. ExPASy Swiss-Prot
Match: Q8QGV2 (Wee1-like protein kinase 1-B OS=Xenopus laevis OX=8355 GN=wee1-b PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.3e-49
Identity = 102/277 (36.82%), Postives = 159/277 (57.40%), Query Frame = 0


Query: 237 SRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAA 296
           SRY T+FHE+++IG+G F  VFK +KR+DGC+YA+K+S +PL   V+ + AL EV A A 
Sbjct: 242 SRYATEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAV 301

Query: 297 LGSHENIVGYYSSWFENEQLYIQMELC------DFSLSIGRYSHPFSEVDALRALYQIAK 356
           LG H ++V YYS+W E++ + IQ E C      D      R    F+E +    L Q+A+
Sbjct: 302 LGQHPHVVRYYSAWAEDDHMLIQNEYCNGGSLSDVISENYRTMQYFTEPELKDLLLQVAR 361

Query: 357 ALLFVHEKGVAHLDVKPDNIYVK--------------------DGVYKLGDFGCVTLLDK 416
            L ++H   + H+D+KP NI++                       +YK+GD G VT +  
Sbjct: 362 GLKYIHSMSLVHMDIKPSNIFISRTTLPNTAVEEADDEECGSGKVIYKIGDLGHVTRV-S 421

Query: 417 SLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIARGSTLPET--HFMNLKEGKL 476
           S  +EEGD+R++  E+L E Y +L K DIF+L   ++  A     P     +  +++GKL
Sbjct: 422 SPQVEEGDSRFLANEVLQENYTHLAKADIFALALTVWSAAGAEPFPTNGDQWHEIRQGKL 481

Query: 477 PLLPG-HSLQFQNLIKAMVDPDPTRRPSAREVLENRI 485
           P +P   S +F +LIK M+ PDP +RPS+  ++++ +
Sbjct: 482 PRVPQLLSQEFVDLIKLMISPDPEKRPSSVALVKHSV 517

[Close]

BLAST of Lsi02G023760 vs. ExPASy Swiss-Prot
Match: Q1LX51 (Wee1-like protein kinase 2 OS=Danio rerio OX=7955 GN=wee2 PE=2 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 7.4e-49
Identity = 111/289 (38.41%), Postives = 164/289 (56.75%), Query Frame = 0


Query: 236 LSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALA 295
           LSRY ++F E+  IG G F  V++ +KR+DGC+YA+K+S RP+      + AL EV A A
Sbjct: 181 LSRYESEFLELACIGVGEFGSVYRCVKRLDGCMYAIKRSRRPIAGSANEQLALKEVYAHA 240

Query: 296 ALGSHENIVGYYSSWFENEQLYIQMELCD-----FSLSIGRYSHPFSEVDALR-ALYQIA 355
            LG H ++V YYS+W E++ + IQ E CD      +++  R    F  V  LR  L Q++
Sbjct: 241 VLGHHPHVVRYYSAWAEDDHMIIQNEYCDGGSLHDAITEKREQGEFFSVPELRDLLLQVS 300

Query: 356 KALLFVHEKGVAHLDVKPDNIYV----------------------KDGVYKLGDFGCVTL 415
             L ++H  G+ HLD+KP NI++                         VYK+GD G VT 
Sbjct: 301 MGLKYIHNSGLVHLDIKPSNIFICRRSTLSAGGEGDSEEEDESHSSGVVYKIGDLGHVTS 360

Query: 416 LDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIARGSTLPET--HFMNLKE 475
           +  S  +EEGD+R++  E+L E Y +L K DIF+LG  +   A  S LP+    +  L++
Sbjct: 361 I-SSPQVEEGDSRFLAYEVLREDYTHLPKADIFALGLTVLLAAGASPLPQNGDDWHRLRQ 420

Query: 476 GKLPLLPGHSLQ--FQNLIKAMVDPDPTRRPSAREVLENRIFDKVRNHK 493
           G+LP LP H L   F++L+K+++DPDPT RPSA  +  + +  K R  K
Sbjct: 421 GELPNLP-HELPALFKDLLKSLLDPDPTARPSATALCRHDVLCKERAGK 467

[Close]

BLAST of Lsi02G023760 vs. ExPASy Swiss-Prot
Match: P54350 (Wee1-like protein kinase OS=Drosophila melanogaster OX=7227 GN=Wee1 PE=1 SV=2)

HSP 1 Score: 195.7 bits (496), Expect = 1.3e-48
Identity = 106/290 (36.55%), Postives = 157/290 (54.14%), Query Frame = 0


Query: 236 LSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALA 295
           +SR+  +F ++  IG G F  VF+ + R+DGC+YA+K+S +P+      +RAL EV A A
Sbjct: 232 ISRFKREFMQVNVIGVGEFGVVFQCVNRLDGCIYAIKKSKKPVAGSSFEKRALNEVWAHA 291

Query: 296 ALGSHENIVGYYSSWFENEQLYIQMELCD-FSLSIGRYSHPFSEVDALRALYQIAKALLF 355
            LG H+N+V YYS+W E++ + IQ E CD  SL      H   E +    L  + + L +
Sbjct: 292 VLGKHDNVVRYYSAWAEDDHMLIQNEFCDGGSLHARIQDHCLGEAELKIVLMHVIEGLRY 351

Query: 356 VHEKGVAHLDVKPDNIY-----------------VKDG-----------------VYKLG 415
           +H   + H+D+KP+NI+                  KD                   YK+G
Sbjct: 352 IHSNDLVHMDLKPENIFSTMNPNAHKLVEVQPQQTKDDDGMDSVYEELRHSENLVTYKIG 411

Query: 416 DFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIARGSTLPET-- 475
           D G VT + K   +EEGD RY+P+EIL+E Y  L K DIFSLG  ++E A G  LP+   
Sbjct: 412 DLGHVTSV-KEPYVEEGDCRYLPKEILHEDYSNLFKADIFSLGITLFEAAGGGPLPKNGP 471

Query: 476 HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENRIFDKV 489
            + NL++GK+P+LP  S  F  LI  M+ P P +RP+++ +  + I   V
Sbjct: 472 EWHNLRDGKVPILPSLSRDFNELIAQMMHPYPDKRPTSQSIFSHPILSAV 520

[Close]

BLAST of Lsi02G023760 vs. ExPASy TrEMBL
Match: A0A1S3BRY6 (wee1-like protein kinase OS=Cucumis melo OX=3656 GN=LOC103492846 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 5.0e-266
Identity = 461/494 (93.32%), Postives = 472/494 (95.55%), Query Frame = 0


Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLP 60
           MRRKSKTLRTTM TRRKKMKGT+TRHLQVQLG+VSLAPL LHRSSS+AA     CFQ+LP
Sbjct: 1   MRRKSKTLRTTMGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSSSAA-----CFQNLP 60

Query: 61  ESEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120
           ESEAP  VN SDALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP
Sbjct: 61  ESEAPSTVNVSDALPSSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120

Query: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKE 180
           KSPEKINTVKTKRKIHE+VLV PLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKKE
Sbjct: 121 KSPEKINTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKE 180

Query: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240
           +YVSQSAVALRCRVMPPPCMKNPYL DASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH
Sbjct: 181 SYVSQSAVALRCRVMPPPCMKNPYLGDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240

Query: 241 TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSH 300
           TDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQD ERRRALMEVQALAALGSH
Sbjct: 241 TDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSH 300

Query: 301 ENIVGYYSSWFENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGV 360
           ENIVGYY+SWFENEQLYIQMELCD SLS+G YSHPFSEVDALRALYQIAKALLFVHEKG+
Sbjct: 301 ENIVGYYTSWFENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGI 360

Query: 361 AHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420
           AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF
Sbjct: 361 AHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420

Query: 421 SLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVL 480
           SLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+PTRRPSAREVL
Sbjct: 421 SLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVL 480

Query: 481 ENRIFDKVRNHKQS 495
           EN IFDKVR HKQS
Sbjct: 481 ENPIFDKVRTHKQS 489

[Close]

BLAST of Lsi02G023760 vs. ExPASy TrEMBL
Match: A0A0A0K5B3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G395770 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.7e-264
Identity = 456/494 (92.31%), Postives = 470/494 (95.14%), Query Frame = 0


Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLP 60
           MRRKSKTLRTTM  RRKKMKGT+TRHLQVQLG+VSLAPLHL+RSS     SSA+CF  LP
Sbjct: 1   MRRKSKTLRTTMGARRKKMKGTVTRHLQVQLGKVSLAPLHLNRSS-----SSALCFPSLP 60

Query: 61  ESEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120
           ESE P  VN SD LPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP
Sbjct: 61  ESEPPSTVNFSDPLPSSSHNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120

Query: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKE 180
           KSPEK+NTVKTKRKIHE+VLV PLSPSLSGCEQVVELGND+FGSDDVDMEKET AMPKKE
Sbjct: 121 KSPEKVNTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDIFGSDDVDMEKETTAMPKKE 180

Query: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240
           NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH
Sbjct: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240

Query: 241 TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSH 300
           TDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQD ERRRALMEVQALAALG+H
Sbjct: 241 TDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGAH 300

Query: 301 ENIVGYYSSWFENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGV 360
           ENIVGYY+SWFENEQLYIQMELCD SLS+GRYSHPFSEVDALRALYQIAKALLFVHEKG+
Sbjct: 301 ENIVGYYTSWFENEQLYIQMELCDCSLSMGRYSHPFSEVDALRALYQIAKALLFVHEKGI 360

Query: 361 AHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420
           AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF
Sbjct: 361 AHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420

Query: 421 SLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVL 480
           SLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP+RRPSAREV+
Sbjct: 421 SLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPSRRPSAREVI 480

Query: 481 ENRIFDKVRNHKQS 495
           EN IFDKVR HKQS
Sbjct: 481 ENPIFDKVRTHKQS 489

[Close]

BLAST of Lsi02G023760 vs. ExPASy TrEMBL
Match: A0A5A7VN66 (Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G002220 PE=3 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 4.8e-261
Identity = 450/483 (93.17%), Postives = 461/483 (95.45%), Query Frame = 0


Query: 12  MTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLPESEAPPMVNAS 71
           M TRRKKMKGT+TRHLQVQLG+VSLAPL LHRSSS+AA     CFQ+LPESEAP  VN S
Sbjct: 1   MGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSSSAA-----CFQNLPESEAPSTVNVS 60

Query: 72  DALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKT 131
           DALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKT
Sbjct: 61  DALPSSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKT 120

Query: 132 KRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKENYVSQSAVALR 191
           KRKIHE+VLV PLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKKE+YVSQSAVALR
Sbjct: 121 KRKIHENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVSQSAVALR 180

Query: 192 CRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKQIGT 251
           CRVMPPPCMKNPYL DASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIK IGT
Sbjct: 181 CRVMPPPCMKNPYLGDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKLIGT 240

Query: 252 GNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHENIVGYYSSWF 311
           GNFSRVFKVLKRIDGCLYAVKQSTRPLNQD ERRRALMEVQALAALGSHENIVGYY+SWF
Sbjct: 241 GNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVGYYTSWF 300

Query: 312 ENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHLDVKPDNIY 371
           ENEQLYIQMELCD SLS+G YSHPFSEVDALRALYQIAKALLFVHEKG+AHLDVKPDNIY
Sbjct: 301 ENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIY 360

Query: 372 VKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIAR 431
           +KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI R
Sbjct: 361 IKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVR 420

Query: 432 GSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENRIFDKVRNH 491
           G TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+PTRRPSAREVLEN IFDKVR H
Sbjct: 421 GFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVLENPIFDKVRTH 478

Query: 492 KQS 495
           KQS
Sbjct: 481 KQS 478

[Close]

BLAST of Lsi02G023760 vs. ExPASy TrEMBL
Match: A0A6J1H582 (wee1-like protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111460604 PE=3 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.4e-254
Identity = 443/494 (89.68%), Postives = 455/494 (92.11%), Query Frame = 0


Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLP 60
           MRRKSKTLRTTMT  RKKMKGTLTRHLQVQLGQVSLAPLH  RSSS AA     CFQDLP
Sbjct: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQRSSSTAA-----CFQDLP 60

Query: 61  ESEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120
           ESEA   VN S ALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD  KE  PCP
Sbjct: 61  ESEAMATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCP 120

Query: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKE 180
           KSPEKINTVKTKRKI + VLVHPLSPSLSG EQVVELGND FG+DD DMEKETMAMPKK+
Sbjct: 121 KSPEKINTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQ 180

Query: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240
           NYVSQSAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYH
Sbjct: 181 NYVSQSAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYH 240

Query: 241 TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSH 300
           TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPL+QD+ERRRALMEVQALAALGSH
Sbjct: 241 TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLHQDIERRRALMEVQALAALGSH 300

Query: 301 ENIVGYYSSWFENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGV 360
           ENIVGYYSSWFENEQLYIQMELCD SLS GRYSHPFSE DAL+AL+QIAKALLF+HEKGV
Sbjct: 301 ENIVGYYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGV 360

Query: 361 AHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420
            HLDVKPDNIYVKDGVYKLGDFGC TLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF
Sbjct: 361 VHLDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420

Query: 421 SLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVL 480
           SLGAAIYE+ RGST PETHFMNLKEGKLPL P HSLQFQNLIKAMVDPDP RRPSAREVL
Sbjct: 421 SLGAAIYEVVRGSTFPETHFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREVL 480

Query: 481 ENRIFDKVRNHKQS 495
           +N IFDKVRNH +S
Sbjct: 481 QNPIFDKVRNHPKS 489

[Close]

BLAST of Lsi02G023760 vs. ExPASy TrEMBL
Match: A0A5D3D2H5 (Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00240 PE=4 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.2e-253
Identity = 437/471 (92.78%), Postives = 447/471 (94.90%), Query Frame = 0


Query: 24  TRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLPESEAPPMVNASDALPSSSPNFED 83
           T H  VQLG+VSLAPL LHRSSS+AA     CFQ+LPESEAP  VN SDALPSSS NFED
Sbjct: 166 TFHNVVQLGKVSLAPLQLHRSSSSAA-----CFQNLPESEAPSTVNVSDALPSSSSNFED 225

Query: 84  RDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKTKRKIHESVLVHP 143
           RDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKTKRKIHE+VLV P
Sbjct: 226 RDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKTKRKIHENVLVRP 285

Query: 144 LSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKENYVSQSAVALRCRVMPPPCMKNP 203
           LSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKKE+YVSQSAVALRCRVMPPPCMKNP
Sbjct: 286 LSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVSQSAVALRCRVMPPPCMKNP 345

Query: 204 YLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVLKR 263
           YL DASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIK IGTGNFSRVFKVLKR
Sbjct: 346 YLGDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKLIGTGNFSRVFKVLKR 405

Query: 264 IDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHENIVGYYSSWFENEQLYIQMELC 323
           IDGCLYAVKQSTRPLNQD ERRRALMEVQALAALGSHENIVGYY+SWFENEQLYIQMELC
Sbjct: 406 IDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVGYYTSWFENEQLYIQMELC 465

Query: 324 DFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVYKLGDFG 383
           D SLS+G YSHPFSEVDALRALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGVYKLGDFG
Sbjct: 466 DCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGVYKLGDFG 525

Query: 384 CVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIARGSTLPETHFMNL 443
           CVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI RG TLPETHFMNL
Sbjct: 526 CVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVRGFTLPETHFMNL 585

Query: 444 KEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENRIFDKVRNHKQS 495
           KEGKLPLLPGHSLQFQNLIKAMVDP+PTRRPSAREVLEN IFDKVR HKQS
Sbjct: 586 KEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVLENPIFDKVRTHKQS 631

[Close]

BLAST of Lsi02G023760 vs. NCBI nr
Match: XP_038876752.1 (wee1-like protein kinase [Benincasa hispida])

HSP 1 Score: 963.8 bits (2490), Expect = 5.8e-277
Identity = 476/492 (96.75%), Postives = 483/492 (98.17%), Query Frame = 0


Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLP 60
           MRRKS+TLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSS+AAAS+A CFQDLP
Sbjct: 1   MRRKSRTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSSAAASTAACFQDLP 60

Query: 61  ESEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120
           ESEAPP VNASDALPS SPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSN+ECIPCP
Sbjct: 61  ESEAPPTVNASDALPSPSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNQECIPCP 120

Query: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKE 180
           KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKK+
Sbjct: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKQ 180

Query: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240
           NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH
Sbjct: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240

Query: 241 TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSH 300
           TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQD+ERRRALMEVQALAALGSH
Sbjct: 241 TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDIERRRALMEVQALAALGSH 300

Query: 301 ENIVGYYSSWFENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGV 360
           ENIVGYYSSWFENEQLYIQMELCD SLSIGRYSHPF EVDALRALYQIAKALLFVHEKGV
Sbjct: 301 ENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFFEVDALRALYQIAKALLFVHEKGV 360

Query: 361 AHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420
           AHLDVKPDNIYVKDGV+KLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF
Sbjct: 361 AHLDVKPDNIYVKDGVFKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420

Query: 421 SLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVL 480
           SLGAAIYEI RGSTLPE HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVL
Sbjct: 421 SLGAAIYEIVRGSTLPEMHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVL 480

Query: 481 ENRIFDKVRNHK 493
           EN IFDKVRNHK
Sbjct: 481 ENPIFDKVRNHK 492

[Close]

BLAST of Lsi02G023760 vs. NCBI nr
Match: XP_008451611.1 (PREDICTED: wee1-like protein kinase [Cucumis melo])

HSP 1 Score: 926.4 bits (2393), Expect = 1.0e-265
Identity = 461/494 (93.32%), Postives = 472/494 (95.55%), Query Frame = 0


Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLP 60
           MRRKSKTLRTTM TRRKKMKGT+TRHLQVQLG+VSLAPL LHRSSS+AA     CFQ+LP
Sbjct: 1   MRRKSKTLRTTMGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSSSAA-----CFQNLP 60

Query: 61  ESEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120
           ESEAP  VN SDALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP
Sbjct: 61  ESEAPSTVNVSDALPSSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120

Query: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKE 180
           KSPEKINTVKTKRKIHE+VLV PLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKKE
Sbjct: 121 KSPEKINTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKE 180

Query: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240
           +YVSQSAVALRCRVMPPPCMKNPYL DASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH
Sbjct: 181 SYVSQSAVALRCRVMPPPCMKNPYLGDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240

Query: 241 TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSH 300
           TDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQD ERRRALMEVQALAALGSH
Sbjct: 241 TDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSH 300

Query: 301 ENIVGYYSSWFENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGV 360
           ENIVGYY+SWFENEQLYIQMELCD SLS+G YSHPFSEVDALRALYQIAKALLFVHEKG+
Sbjct: 301 ENIVGYYTSWFENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGI 360

Query: 361 AHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420
           AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF
Sbjct: 361 AHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420

Query: 421 SLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVL 480
           SLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+PTRRPSAREVL
Sbjct: 421 SLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVL 480

Query: 481 ENRIFDKVRNHKQS 495
           EN IFDKVR HKQS
Sbjct: 481 ENPIFDKVRTHKQS 489

[Close]

BLAST of Lsi02G023760 vs. NCBI nr
Match: XP_004136046.1 (wee1-like protein kinase isoform X2 [Cucumis sativus] >KGN44910.1 hypothetical protein Csa_015648 [Cucumis sativus])

HSP 1 Score: 920.6 bits (2378), Expect = 5.7e-264
Identity = 456/494 (92.31%), Postives = 470/494 (95.14%), Query Frame = 0


Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLP 60
           MRRKSKTLRTTM  RRKKMKGT+TRHLQVQLG+VSLAPLHL+RSS     SSA+CF  LP
Sbjct: 1   MRRKSKTLRTTMGARRKKMKGTVTRHLQVQLGKVSLAPLHLNRSS-----SSALCFPSLP 60

Query: 61  ESEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120
           ESE P  VN SD LPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP
Sbjct: 61  ESEPPSTVNFSDPLPSSSHNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120

Query: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKE 180
           KSPEK+NTVKTKRKIHE+VLV PLSPSLSGCEQVVELGND+FGSDDVDMEKET AMPKKE
Sbjct: 121 KSPEKVNTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDIFGSDDVDMEKETTAMPKKE 180

Query: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240
           NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH
Sbjct: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYH 240

Query: 241 TDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSH 300
           TDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQD ERRRALMEVQALAALG+H
Sbjct: 241 TDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGAH 300

Query: 301 ENIVGYYSSWFENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGV 360
           ENIVGYY+SWFENEQLYIQMELCD SLS+GRYSHPFSEVDALRALYQIAKALLFVHEKG+
Sbjct: 301 ENIVGYYTSWFENEQLYIQMELCDCSLSMGRYSHPFSEVDALRALYQIAKALLFVHEKGI 360

Query: 361 AHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420
           AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF
Sbjct: 361 AHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIF 420

Query: 421 SLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVL 480
           SLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP+RRPSAREV+
Sbjct: 421 SLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPSRRPSAREVI 480

Query: 481 ENRIFDKVRNHKQS 495
           EN IFDKVR HKQS
Sbjct: 481 ENPIFDKVRTHKQS 489

[Close]

BLAST of Lsi02G023760 vs. NCBI nr
Match: XP_031744240.1 (wee1-like protein kinase isoform X1 [Cucumis sativus])

HSP 1 Score: 916.0 bits (2366), Expect = 1.4e-262
Identity = 456/495 (92.12%), Postives = 470/495 (94.95%), Query Frame = 0


Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLP 60
           MRRKSKTLRTTM  RRKKMKGT+TRHLQVQLG+VSLAPLHL+RSS     SSA+CF  LP
Sbjct: 1   MRRKSKTLRTTMGARRKKMKGTVTRHLQVQLGKVSLAPLHLNRSS-----SSALCFPSLP 60

Query: 61  ESEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120
           ESE P  VN SD LPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP
Sbjct: 61  ESEPPSTVNFSDPLPSSSHNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCP 120

Query: 121 KSPEKINTVKTKRKIH-ESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKK 180
           KSPEK+NTVKTKRKIH E+VLV PLSPSLSGCEQVVELGND+FGSDDVDMEKET AMPKK
Sbjct: 121 KSPEKVNTVKTKRKIHAENVLVRPLSPSLSGCEQVVELGNDIFGSDDVDMEKETTAMPKK 180

Query: 181 ENYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRY 240
           ENYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRY
Sbjct: 181 ENYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRY 240

Query: 241 HTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGS 300
           HTDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQD ERRRALMEVQALAALG+
Sbjct: 241 HTDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGA 300

Query: 301 HENIVGYYSSWFENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKG 360
           HENIVGYY+SWFENEQLYIQMELCD SLS+GRYSHPFSEVDALRALYQIAKALLFVHEKG
Sbjct: 301 HENIVGYYTSWFENEQLYIQMELCDCSLSMGRYSHPFSEVDALRALYQIAKALLFVHEKG 360

Query: 361 VAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDI 420
           +AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDI
Sbjct: 361 IAHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDI 420

Query: 421 FSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREV 480
           FSLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP+RRPSAREV
Sbjct: 421 FSLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPSRRPSAREV 480

Query: 481 LENRIFDKVRNHKQS 495
           +EN IFDKVR HKQS
Sbjct: 481 IENPIFDKVRTHKQS 490

[Close]

BLAST of Lsi02G023760 vs. NCBI nr
Match: KAA0068026.1 (wee1-like protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 909.8 bits (2350), Expect = 1.0e-260
Identity = 450/483 (93.17%), Postives = 461/483 (95.45%), Query Frame = 0


Query: 12  MTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLPESEAPPMVNAS 71
           M TRRKKMKGT+TRHLQVQLG+VSLAPL LHRSSS+AA     CFQ+LPESEAP  VN S
Sbjct: 1   MGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSSSAA-----CFQNLPESEAPSTVNVS 60

Query: 72  DALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKT 131
           DALPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKT
Sbjct: 61  DALPSSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKT 120

Query: 132 KRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKENYVSQSAVALR 191
           KRKIHE+VLV PLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKKE+YVSQSAVALR
Sbjct: 121 KRKIHENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVSQSAVALR 180

Query: 192 CRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKQIGT 251
           CRVMPPPCMKNPYL DASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIK IGT
Sbjct: 181 CRVMPPPCMKNPYLGDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHEIKLIGT 240

Query: 252 GNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHENIVGYYSSWF 311
           GNFSRVFKVLKRIDGCLYAVKQSTRPLNQD ERRRALMEVQALAALGSHENIVGYY+SWF
Sbjct: 241 GNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVGYYTSWF 300

Query: 312 ENEQLYIQMELCDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHLDVKPDNIY 371
           ENEQLYIQMELCD SLS+G YSHPFSEVDALRALYQIAKALLFVHEKG+AHLDVKPDNIY
Sbjct: 301 ENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIY 360

Query: 372 VKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIAR 431
           +KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI R
Sbjct: 361 IKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVR 420

Query: 432 GSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENRIFDKVRNH 491
           G TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+PTRRPSAREVLEN IFDKVR H
Sbjct: 421 GFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVLENPIFDKVRTH 478

Query: 492 KQS 495
           KQS
Sbjct: 481 KQS 478

[Close]

BLAST of Lsi02G023760 vs. TAIR 10
Match: AT1G02970.1 (WEE1 kinase homolog )

HSP 1 Score: 568.9 bits (1465), Expect = 3.9e-162
Identity = 294/492 (59.76%), Postives = 369/492 (75.00%), Query Frame = 0


Query: 2   RRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAASSAVCFQDLPE 61
           R+   T++T  + + +KM+GTL RH  +Q GQ+S        SS+ A+++    FQ L +
Sbjct: 13  RKTQGTIKTRASKKIRKMEGTLERHSLLQFGQLSKISFENRPSSNVASSA----FQGLLD 72

Query: 62  SEAPPMVNASDALPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPK 121
           S++  + N   +  S + N  ++DFILSQDFFCTPDYITPDNQNL++G+D +K+  PCP+
Sbjct: 73  SDSSELRNQLGSADSDA-NCGEKDFILSQDFFCTPDYITPDNQNLMSGLDISKDHSPCPR 132

Query: 122 SPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKEN 181
           SP K+NTVK+KR   ES   +  + + S   +V E  ND   +D+V  +K      ++  
Sbjct: 133 SPVKLNTVKSKRCRQESFTGNHSNSTWSSKHRVDEQENDDIDTDEVMGDKLQANQTERTG 192

Query: 182 YVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHT 241
           YVSQ+AVALRCR MPPPC+KNPY+ + S+   DPFG+QR+KCA F P   SGDGLSRY T
Sbjct: 193 YVSQAAVALRCRAMPPPCLKNPYVLNQSETATDPFGHQRSKCASFLPVSTSGDGLSRYLT 252

Query: 242 DFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHE 301
           DFHEI+QIG G+FSRVFKVLKR+DGCLYAVK STR L  D ERR+A+MEVQALAALG HE
Sbjct: 253 DFHEIRQIGAGHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQALAALGFHE 312

Query: 302 NIVGYYSSWFENEQLYIQMELCDFSLSI--GRYSHPFSEVDALRALYQIAKALLFVHEKG 361
           NIVGYYSSWFENEQLYIQ+ELCD SLS    + S   SE + L  ++QIAKAL FVHEKG
Sbjct: 313 NIVGYYSSWFENEQLYIQLELCDHSLSALPKKSSLKVSEREILVIMHQIAKALHFVHEKG 372

Query: 362 VAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDI 421
           +AHLDVKPDNIY+K+GV KLGDFGC T LDKSLP+EEGDARYMPQEILNE Y++LDKVDI
Sbjct: 373 IAHLDVKPDNIYIKNGVCKLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYEHLDKVDI 432

Query: 422 FSLGAAIYEIARGSTLPET--HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAR 481
           FSLG  +YE+ +GS L E+    +N+KEGKLPLLPGHSLQ Q L+K M+D DP RRPSAR
Sbjct: 433 FSLGVTVYELIKGSPLTESRNQSLNIKEGKLPLLPGHSLQLQQLLKTMMDRDPKRRPSAR 492

Query: 482 EVLENRIFDKVR 490
           E+L++ +FD++R
Sbjct: 493 ELLDHPMFDRIR 499

[Close]

BLAST of Lsi02G023760 vs. TAIR 10
Match: AT4G19110.1 (Protein kinase superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 8.8e-21
Identity = 77/279 (27.60%), Postives = 129/279 (46.24%), Query Frame = 0


Query: 246 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHENIVG 305
           IK++G G F  V++ + +  G + A+K+  +      +    L EV++L  + +H NIV 
Sbjct: 7   IKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKY-YSWDECINLREVKSLRRM-NHPNIVK 66

Query: 306 YYSSWFENEQLYIQMEL--CDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHL 365
                 EN+ LY   E   C+    +      F+E D     +Q+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYMHQRGYFHR 126

Query: 366 DVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 425
           D+KP+N+ V   + K+ DFG    ++ S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 426 LGA-------------------AIYEIAR--GSTLPET--------HFMNLKEGKLP--- 485
           +GA                    IY+I    G+   ET        + +N +  +LP   
Sbjct: 187 MGAIMAELLSLRPIFPGASEADEIYKICSVIGTPTEETWLEGLNLANTINYQFPQLPGVP 246

[Close]

BLAST of Lsi02G023760 vs. TAIR 10
Match: AT4G19110.2 (Protein kinase superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 8.8e-21
Identity = 77/279 (27.60%), Postives = 129/279 (46.24%), Query Frame = 0


Query: 246 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHENIVG 305
           IK++G G F  V++ + +  G + A+K+  +      +    L EV++L  + +H NIV 
Sbjct: 7   IKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKY-YSWDECINLREVKSLRRM-NHPNIVK 66

Query: 306 YYSSWFENEQLYIQMEL--CDFSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHL 365
                 EN+ LY   E   C+    +      F+E D     +Q+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYMHQRGYFHR 126

Query: 366 DVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 425
           D+KP+N+ V   + K+ DFG    ++ S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 426 LGA-------------------AIYEIAR--GSTLPET--------HFMNLKEGKLP--- 485
           +GA                    IY+I    G+   ET        + +N +  +LP   
Sbjct: 187 MGAIMAELLSLRPIFPGASEADEIYKICSVIGTPTEETWLEGLNLANTINYQFPQLPGVP 246

[Close]

BLAST of Lsi02G023760 vs. TAIR 10
Match: AT5G45430.1 (Protein kinase superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 6.3e-19
Identity = 77/280 (27.50%), Postives = 122/280 (43.57%), Query Frame = 0


Query: 246 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHENIVG 305
           +K++G G F  V++ + +    + A+K+  +      E    L EV++L+ + +H NIV 
Sbjct: 7   LKEVGDGTFGNVWRAVNKQTNEVVAIKRMKKKY-FSWEECVNLREVKSLSRM-NHPNIVK 66

Query: 306 YYSSWFENEQLYIQMELCD---FSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAH 365
                 EN+ LY   E  +   + L   R  H F+E D     +Q+ + L ++H++G  H
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRPKH-FAESDIRNWCFQVFQGLSYMHQRGYFH 126

Query: 366 LDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIF 425
            D+KP+N+ V   V K+ D G    +D S P  E      Y   E+L + Y Y  KVD++
Sbjct: 127 RDLKPENLLVSKDVIKIADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKVDMW 186

Query: 426 SLGA-------------------AIYEIAR--GSTLPETHFMNLK-----EGKLPLLPG- 485
           ++GA                    IY+I    GS   ET    L        + P  PG 
Sbjct: 187 AMGAIMAELLSLRPLFPGASEADEIYKICSVIGSPTEETWLEGLNLASVINYQFPQFPGV 246

[Close]

BLAST of Lsi02G023760 vs. TAIR 10
Match: AT5G45430.2 (Protein kinase superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 6.3e-19
Identity = 77/280 (27.50%), Postives = 122/280 (43.57%), Query Frame = 0


Query: 246 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDVERRRALMEVQALAALGSHENIVG 305
           +K++G G F  V++ + +    + A+K+  +      E    L EV++L+ + +H NIV 
Sbjct: 7   LKEVGDGTFGNVWRAVNKQTNEVVAIKRMKKKY-FSWEECVNLREVKSLSRM-NHPNIVK 66

Query: 306 YYSSWFENEQLYIQMELCD---FSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAH 365
                 EN+ LY   E  +   + L   R  H F+E D     +Q+ + L ++H++G  H
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRPKH-FAESDIRNWCFQVFQGLSYMHQRGYFH 126

Query: 366 LDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIF 425
            D+KP+N+ V   V K+ D G    +D S P  E      Y   E+L + Y Y  KVD++
Sbjct: 127 RDLKPENLLVSKDVIKIADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKVDMW 186

Query: 426 SLGA-------------------AIYEIAR--GSTLPETHFMNLK-----EGKLPLLPG- 485
           ++GA                    IY+I    GS   ET    L        + P  PG 
Sbjct: 187 AMGAIMAELLSLRPLFPGASEADEIYKICSVIGSPTEETWLEGLNLASVINYQFPQFPGV 246

The following BLAST results are available for this feature:

BLAST of Lsi02G023760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L4H0	5.5e-161	59.76	Wee1-like protein kinase OS=Arabidopsis thaliana OX=3702 GN=WEE1 PE=1 SV=1	[more]
Q6Z829	1.5e-137	55.72	Wee1-like protein kinase OS=Oryza sativa subsp. japonica OX=39947 GN=WEE1 PE=3 S...	[more]
Q8QGV2	4.3e-49	36.82	Wee1-like protein kinase 1-B OS=Xenopus laevis OX=8355 GN=wee1-b PE=1 SV=1	[more]
Q1LX51	7.4e-49	38.41	Wee1-like protein kinase 2 OS=Danio rerio OX=7955 GN=wee2 PE=2 SV=2	[more]
P54350	1.3e-48	36.55	Wee1-like protein kinase OS=Drosophila melanogaster OX=7227 GN=Wee1 PE=1 SV=2	[more]

BLAST of Lsi02G023760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BRY6	5.0e-266	93.32	wee1-like protein kinase OS=Cucumis melo OX=3656 GN=LOC103492846 PE=3 SV=1	[more]
A0A0A0K5B3	2.7e-264	92.31	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G395...	[more]
A0A5A7VN66	4.8e-261	93.17	Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A6J1H582	4.4e-254	89.68	wee1-like protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111460604 PE=3 SV=1	[more]
A0A5D3D2H5	2.2e-253	92.78	Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]

BLAST of Lsi02G023760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876752.1	5.8e-277	96.75	wee1-like protein kinase [Benincasa hispida]	[more]
XP_008451611.1	1.0e-265	93.32	PREDICTED: wee1-like protein kinase [Cucumis melo]	[more]
XP_004136046.1	5.7e-264	92.31	wee1-like protein kinase isoform X2 [Cucumis sativus] >KGN44910.1 hypothetical p...	[more]
XP_031744240.1	1.4e-262	92.12	wee1-like protein kinase isoform X1 [Cucumis sativus]	[more]
KAA0068026.1	1.0e-260	93.17	wee1-like protein kinase [Cucumis melo var. makuwa]	[more]

BLAST of Lsi02G023760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02970.1	3.9e-162	59.76	WEE1 kinase homolog	[more]
AT4G19110.1	8.8e-21	27.60	Protein kinase superfamily protein	[more]
AT4G19110.2	8.8e-21	27.60	Protein kinase superfamily protein	[more]
AT5G45430.1	6.3e-19	27.50	Protein kinase superfamily protein	[more]
AT5G45430.2	6.3e-19	27.50	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 243..485 e-value: 2.0E-42 score: 156.9
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 243..484 e-value: 4.1E-46 score: 157.5
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 243..485 score: 35.749847
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 322..490 e-value: 8.7E-36 score: 125.1
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 233..321 e-value: 1.0E-31 score: 110.9
None	No IPR available	PANTHER	PTHR11042	EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED	coord: 414..490
None	No IPR available	PANTHER	PTHR11042:SF155	BNAA08G27310D PROTEIN	coord: 414..490 coord: 19..401
None	No IPR available	PANTHER	PTHR11042	EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED	coord: 19..401
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 360..372
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 249..272
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 236..488

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G023760.1	Lsi02G023760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1902750	negative regulation of cell cycle G2/M phase transition
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004713	protein tyrosine kinase activity
molecular_function	GO:0004672	protein kinase activity