Lsi02G023660 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G023660
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPeptide chain release factor 1
Locationchr02: 30316920 .. 30322207 (+)
RNA-Seq ExpressionLsi02G023660
SyntenyLsi02G023660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCAAGCTGGCCGATCCAGATATTGTCTCGAACCCCACAGAGTATCAAAAACTTGCTCAATCAATGTCAGAACTTGACGAGGTATGGCAAGATGTCTGCCTCCAATACAATAATTTATGTGGAACAGTTAGAATGACCTCTTTTATCTTTTCTTCCTTTCTTCCTCTAATATGTTTCTTTCTACCAACTTACTCTTGGTTGGTTGGTGTGGATTGGCCGGACAGGTGGTATCGACTTTCAGAAAATTCAAGGAATGTGAAAAGCAGTTAGAGGAGGCCAAAGGTTGACTTCTAATTAGTTCTTCTTAAATAAGTTTCTCAACATAAAGTTTTTTGAATTGATCTTAGTTTCTTCTTCTTTGTCGTGATTATCATGTCAGCCTTATCAAAAGAGAGTGGGAATGATGAAGATATGGTAGAGATGATTGCTTTAGAAATCAGTTCTTTATCCAGTCAACTCAAAGATACCGAGGAGAAGCTTAAGGTGCTTTTGGTTCATTTTACATGTGTGTGTTCATTTTGGATCATGACGAGCTTTTATAATGTTAACATGTGTTTAAACCTATTTCTCGTCATTCTGTTTTCCTGTTTGGGTGGGGTGGAGGTCCTTGATTTTCAATCCTCACAGTTCATGGTTTAAAATTTTCATTTTCTGCTTTTAATGAGCTTCTTTACAGATATACATGTCTAAATGCGTTATTTGTTTAATTTCTTTAGCTATATTCATTAATGCTTAATATTTTATCTTCTTATTAAAGGTAGAATCATTTAATTACTGAAACAAATAGGTAATGAAATTAGAGTTTGGTTCTTTACTGATGGTCAGATCTTTACAAGCAAATATCGTGCTTTTCTTGTCATTCTTACAGTGATTGTTTTCTTTTGTTGAGGACTTTCTTTATTTCCTGGTATTCATCAATGGAAAAAAAAGGAAGTTATTTTTTGGTATCTTACAAGTTACATCTTACATTATGTCGTGATTTTCTCTATCAGGTGATGTTGCTTCCATCTGATCCTCTTGATGCAAGAAATATTATGCTTGAAGGTCAACTTCCTCAGTTTCCTGTGAAATTTTATGCCATCTGAATAATGACAAAATTTAACATACATTTGCCGAAGGTTGCATTGATATACTTAATGGACAATTAGAATCCTGTAAATGTAAACTACTTATGTTTATGCCTTATGTTTGTTCTGAACTTCTGATTAAAAACTGTAATTTCCTTCTGCCATGCAGTCCGGGCAGGTACAGGTGGTGATGAAGCTGGAATCTGGGCTGGTGATCTTGTAGGTACAAGTTTCTTTTATCCTTGAAGAACTGTGGATAAATTGTGCACATATTCTTTTTTTTGGTTGCCCATACAATTGGAAACATGAGAAGTATTGGTTATAAAAGAAAATCTCTTGATATCTTCATAAGAAATGCTATGCCAAATTACCATATTCGTGGAGGTGGCCTATGCTCCAATATTCTCCCTACTAGTACTAAATTCTTAGACCTCTTAAGTGTATAGAATAAGCACAGATAGGCTCAGTAAACTTACAAGTTACATGTTCTGAACTCTGAAGTTTTTTCCACATTTGTCCTGCTTTGATTTTACCCTTCCTTTTCACGTATGTATTTTTCTCTATTATTGTTTCTTCTTCTCTAACTCATCTTTTCCAATTATATAATAGGTCCGTATGTACCAGAAATACAGTGAGCGGAATTCTTGGAAGTTTTCCCCTGTTTCAAGTTCTGAGGTATGTGTCTCCAAACATTCCAGAAGTAAACATCCTAGTGCCTTCTGTACGTGTTAAAGAATTTCCGCTGTTCACTTTTCAGGCAGAAAAAGGTGGATTCAAAACTTATGTGTTGGAAATCAAAGGACAACGTGTCTACAGTAAATTAAAATACGAGTCCGGTGTTCACCGGGTTCAACGTGTTCCTCAAACAGAAACGCAGGGCCGTGTACATACTTCTACTGCTACAGTCGCCATCATGCCAGAGGTAAACTATTTCATAAATTTACTAATGAATTGTGAAATAAAGTTGTCTGATTCTTGTCAAAGATGAATTGCATGCCTCAATCAGACTAATGTCTAGTAGAATATTACGTGATTTTGCTAATTTTGTTCTGGACAGTTGACTAATGTTAATTCTTTTTCCCCTTTTCTTTATAGGTCCCTGATCTATTTCTTGTGCTCCATTTTAGCTTTTAACAATTATAAGATGTGGGATTGCTAATCAAAATGTTCTGACATATCACACCAGTGCCTTAACATGAAAATCTGAGTGCATAACTGTAGATTTATCCTTATATATGTGCAGTTAATATTGATAATCTTCATACTGTTTCTGTAGGAGTACTAACATTTTGGATATGATAATTCCCCAAATTTGACTTTGTTGTCCTCTGCATTCACATCTTGATCAGCTATACTTTATCTTGATTGGTTGAGCTTATGCTATAATTGCATCTAGGCAGATGAAGTTGAGGTAGTGATTGATCCAAAAGACATTGAACTAACTACTGCAAGATCTGGGGGCGCTGGAGGTATAAATCTACACAGTTGATTCAAATATTGCTTTCAGTTAGCTCTCTGAATCTGTTCTCATAAAACCAAGAAAAATGGTAGCATAACCTAACCTTGTTATTTATCTCTTGTTGTCAATGATTATGCAGGGCAGAATGTCAACAAAGTGGAGACAGCAATTGATCTTTTCCACAAGCCGACAGGAATCCGCATTTTTTGCACTGAGGAAAGGACTCAACTTCAGAACAAGCATCGAGCTTTTCAATTGCTGCGAGCCAAACTGTGAGCATCATTTACATCCTAACATTTCCCACATTATCGTATACCTTATGTGAATGTGCACTTCATTCATATAATTCCTATATTTTTCTAGTAACAAATAATTGAACTTTGTCCCCACCCTCTGCTTGGCTTAGCATGCCACTTACCCTTCGCCAGCCACCTCCAATCCAGCCTGTAGGTCGGTCAGATCTTTTGTGCAATAAAAGAAAAAAGTTAGTTTTTATGTCCTCCCCCAACCGACAGTCAGCTTCACAGTTACATCTATCCTAATCTACGCTGACTTTCACCCTACCTCCCCACCACAACCAGCCTTTCATAGGGGACAGGGTGATAATTTTGCTGGATTCACTGTTATGTAAATGCATGAATTTAATCCCCTCTCCCTCCTCTTTTTCTTTTCAGTTATGAGATCAAAGTGAGGGAACAGCAGGAGAAGATCAGGAATCAACGGAAATTACAGGTAATTACTGTTTTTTTCCTTTCTAAAATTTTGTTAGCATTTGGAAATATTTAGCAAAGAACACTCTTTCCATATACTTTTGTATGGTCTGCATTTAGACTGTCTTTGAGCTGCTTTCATCAGACGAGAGTTTCTGAAGTTCTTGTAGTTGGTTTGTCATGATTTTGTTTCAATTTGCCCGGGAAATGAAGAGCTGTTACTCTTCATTTTTTCGAGTATTTCCCAGTCTAGAGTAATTTCTTACTAAACACATTATTCCGTTGGTAACATGCAAGATACAATCTGAATGGAATATCAAATACTAGAAATCTTTGACTCCACAATCTCCTACCTAGTGATCTTGGGTGCACAAATACGACTGTTCTACAGCATGATAATTATATTTTAGGATTTTTTTTTCCGTTATTAAGAATCTAGTTCATGATATCACTGAAAGTCCTTTTGATTTTTTGTGTAATTTACAATGTTAAGGCAAGTAGTTGTCACATTATGCAAGGCAATTGTACAAGTTGATGGTTATTCTTATAGAAGCAATCGATTATAAGGGATTTTATTTTATTTTATTTTATTTTAATTTTCAATGTCCTTATTACCTTGTTGTCCTAATTCCTATCTTCTGTCTCCTTGTGGACGTGTTGAAATTTGTTGTCAGAATCTGTTTGAACGATGAAACTTCATGCATACCACTGCTAGTGGTTGTTTCACCTTGAATCTGTTAAAGAAAATTGAAGACCTGATAACTTCTCAATATAATTTAATTAGGCACTCGAATTCTTTTCATTAGTGTATTCCTTATTCTAAAAGGTTCTGTTCCAGGTCGGTACAGGTGCTCGTGCTGAGAAAATTCGGACCTACAATTACAAGGTATGATATTAGTTTGCTTTCTGACTTTGAAAAGATGAAAAGAAATACAGCTTCTGTCATAAAACAAGCCATTCATCCATATGGTACCACAAAGATGACGAAGACAAGAAAGATTTCAATTTTAAAAAAGATGAAGTGTAATACCATTTCGCATTAGACTAGAACTAATAAAACATCAGATACTTCATTACGAGAAGATTTATCAGGAGTGCTTTTTAAAAGTATTTTTCAAATAATTAAAATCTAGGAGCACTTTTCAGTCTTAAGAATTTTTTAATCATTAAAAAAAAAAAAAAAAACCCAAACAATGAAGTATTAACTTGTTAAAAACTTTCGAACAATATACAAAAGGGTTTGAAACATCGAGTATGTTACAGACTCACAAATTACATGTATAAATGATAACCTCACATGTTTGGATTGACTTTCGATAGTTAGAATCAGTTGTATAGCTCATTTTCAGTTTTATTGTTAGAAGGTATTGCCTATTAAAGGGGTATTTCTTTGTAAGCTCTCAATTATTCAATGAAAAGAGCTCTTTCACAGTTTAGTGTAAGATTATTACATGGTATTAAAACAGGTGGTCTAGGAAGTCTTGTGTTCAAACTCTTGTTCTTCTATATATTGCAAGCTCACAAGTGAAGGAAGAATAAGATGATATAATGTTAAATTTACCTTCACCCATCAGCTTAAGCTTTTGGGTCAATTGGTGATTTAGTCGGTGATTTAAGTGCTCTTTTTTCACTTAAATATACTTTTGTATTTAAAAAGTTAATCCACACCTGTTTTGTGAAACTATCATTTCCAGTCTCTATTACAAGCCGGAAATGAAGCAACTGAGCATAATTAATAGAATTCGACCACATAGACTGATGATTTCACTAGAAGTCAATTAACTTTTGGATTTGACTTATGTTAGGACAATAGGGTTACTGACCACCGGTTGAAGATGAACTTCGAGCTCACATCCTTTCTTGAAGGTGATATAGAGAATGCAGTTCAGGTAATATTCTCTCTTTCGTCCACTTTCTCATTCTAATCATCATTTTAATAGAAAGCAAAAAAAATCACATTCTTCCATTTTCTCAATCAAGGCATGTGTTGCCATGGAACAAAAAGAACTCCTAGAAGAACTGGCTCAGTCGGCTGTGGCTTCAGCCAATTGA

mRNA sequence

GTCAAGCTGGCCGATCCAGATATTGTCTCGAACCCCACAGAGTATCAAAAACTTGCTCAATCAATGTCAGAACTTGACGAGGTATGGCAAGATGTCTGCCTCCAATACAATAATTTATGTGGAACAGTTAGAATGACCTCTTTTATCTTTTCTTCCTTTCTTCCTCTAATATGTTTCTTTCTACCAACTTACTCTTGGTTGGTTGGTGTGGATTGGCCGGACAGTTTCTTCTTCTTTGTCGTGATTATCATGTCAGCCTTATCAAAAGAGAGTGGGAATGATGAAGATATGGTAGAGATGATTGCTTTAGAAATCAGTTCTTTATCCAGTCAACTCAAAGATACCGAGGAGAAGCTTAAGGTGATGTTGCTTCCATCTGATCCTCTTGATGCAAGAAATATTATGCTTGAAGTCCGGGCAGGTACAGGTGGTGATGAAGCTGGAATCTGGGCTGGTGATCTTGTCCGTATGTACCAGAAATACAGTGAGCGGAATTCTTGGAAGTTTTCCCCTGTTTCAAGTTCTGAGGCAGAAAAAGGTGGATTCAAAACTTATGTGTTGGAAATCAAAGGACAACGTGTCTACAGTAAATTAAAATACGAGTCCGGTGTTCACCGGGTTCAACGTGTTCCTCAAACAGAAACGCAGGGCCGTGTACATACTTCTACTGCTACAGTCGCCATCATGCCAGAGGCAGATGAAGTTGAGGTAGTGATTGATCCAAAAGACATTGAACTAACTACTGCAAGATCTGGGGGCGCTGGAGGGCAGAATGTCAACAAAGTGGAGACAGCAATTGATCTTTTCCACAAGCCGACAGGAATCCGCATTTTTTGCACTGAGGAAAGGACTCAACTTCAGAACAAGCATCGAGCTTTTCAATTGCTGCGAGCCAAACTTTATGAGATCAAAGTGAGGGAACAGCAGGAGAAGATCAGGAATCAACGGAAATTACAGGTCGGTACAGGTGCTCGTGCTGAGAAAATTCGGACCTACAATTACAAGGACAATAGGGTTACTGACCACCGGTTGAAGATGAACTTCGAGCTCACATCCTTTCTTGAAGGTGATATAGAGAATGCAGTTCAGGCATGTGTTGCCATGGAACAAAAAGAACTCCTAGAAGAACTGGCTCAGTCGGCTGTGGCTTCAGCCAATTGA

Coding sequence (CDS)

GTCAAGCTGGCCGATCCAGATATTGTCTCGAACCCCACAGAGTATCAAAAACTTGCTCAATCAATGTCAGAACTTGACGAGGTATGGCAAGATGTCTGCCTCCAATACAATAATTTATGTGGAACAGTTAGAATGACCTCTTTTATCTTTTCTTCCTTTCTTCCTCTAATATGTTTCTTTCTACCAACTTACTCTTGGTTGGTTGGTGTGGATTGGCCGGACAGTTTCTTCTTCTTTGTCGTGATTATCATGTCAGCCTTATCAAAAGAGAGTGGGAATGATGAAGATATGGTAGAGATGATTGCTTTAGAAATCAGTTCTTTATCCAGTCAACTCAAAGATACCGAGGAGAAGCTTAAGGTGATGTTGCTTCCATCTGATCCTCTTGATGCAAGAAATATTATGCTTGAAGTCCGGGCAGGTACAGGTGGTGATGAAGCTGGAATCTGGGCTGGTGATCTTGTCCGTATGTACCAGAAATACAGTGAGCGGAATTCTTGGAAGTTTTCCCCTGTTTCAAGTTCTGAGGCAGAAAAAGGTGGATTCAAAACTTATGTGTTGGAAATCAAAGGACAACGTGTCTACAGTAAATTAAAATACGAGTCCGGTGTTCACCGGGTTCAACGTGTTCCTCAAACAGAAACGCAGGGCCGTGTACATACTTCTACTGCTACAGTCGCCATCATGCCAGAGGCAGATGAAGTTGAGGTAGTGATTGATCCAAAAGACATTGAACTAACTACTGCAAGATCTGGGGGCGCTGGAGGGCAGAATGTCAACAAAGTGGAGACAGCAATTGATCTTTTCCACAAGCCGACAGGAATCCGCATTTTTTGCACTGAGGAAAGGACTCAACTTCAGAACAAGCATCGAGCTTTTCAATTGCTGCGAGCCAAACTTTATGAGATCAAAGTGAGGGAACAGCAGGAGAAGATCAGGAATCAACGGAAATTACAGGTCGGTACAGGTGCTCGTGCTGAGAAAATTCGGACCTACAATTACAAGGACAATAGGGTTACTGACCACCGGTTGAAGATGAACTTCGAGCTCACATCCTTTCTTGAAGGTGATATAGAGAATGCAGTTCAGGCATGTGTTGCCATGGAACAAAAAGAACTCCTAGAAGAACTGGCTCAGTCGGCTGTGGCTTCAGCCAATTGA

Protein sequence

VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFFLPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKGGFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIENAVQACVAMEQKELLEELAQSAVASAN
Homology
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BLAST of Lsi02G023660 vs. ExPASy Swiss-Prot
Match: Q8RX79 (Peptide chain release factor APG3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APG3 PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 1.8e-143
Identity = 275/385 (71.43%), Postives = 312/385 (81.04%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPD+VSN +EYQKLAQSMSELDEV                    +F  F       
Sbjct: 73  VKLADPDVVSNQSEYQKLAQSMSELDEV------------------VTVFRRFKDCEKQL 132

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
           L +                       L+KE+G+DEDM EMI  EI+SL+ ++++ E++LK
Sbjct: 133 LES---------------------KVLAKEAGDDEDMAEMIGSEINSLTKEIEELEKQLK 192

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           V+LLPSDPLDARNI+LEVRAGTGGDEA IW GDLVRMYQ+YSER+SWKFS VS SEAE G
Sbjct: 193 VLLLPSDPLDARNILLEVRAGTGGDEAAIWTGDLVRMYQRYSERSSWKFSMVSCSEAEHG 252

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           G+KT V+EIKG RVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID
Sbjct: 253 GYKTCVMEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 312

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELT+ARSGGAGGQNVNKVETAIDLFHKP+GIRIFCTEERTQ++NK RAFQLLRAKL
Sbjct: 313 PKDIELTSARSGGAGGQNVNKVETAIDLFHKPSGIRIFCTEERTQIRNKARAFQLLRAKL 372

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQEKIRN+RK QVGTGAR+EKIRTYNYKD+RVTDHRLKMNF LT+FL+G +E+
Sbjct: 373 YEIKVREQQEKIRNERKSQVGTGARSEKIRTYNYKDSRVTDHRLKMNFALTTFLDGALED 418

Query: 361 AVQACVAMEQKELLEELAQSAVASA 386
           AVQAC A+EQKEL+EEL++S  AS+
Sbjct: 433 AVQACAALEQKELMEELSESVAASS 418

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BLAST of Lsi02G023660 vs. ExPASy Swiss-Prot
Match: Q8DMG9 (Peptide chain release factor 1 OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=prfA PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.2e-116
Identity = 223/383 (58.22%), Postives = 272/383 (71.02%), Query Frame = 0 

Query: 2   KLADPDIVSNPTEYQKLAQSMSELDEV------WQDVCLQYNNLCGTVRMTSFIFSSFLP 61
           +LADPD+  NPTE+QK+A+S + L+E       WQ +  Q                    
Sbjct: 23  RLADPDVAVNPTEFQKVARSRAALEETVNAYHEWQSLNQQ-------------------- 82

Query: 62  LICFFLPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDT 121
                                    ++    L KE+ N+ ++ +M   EI+ LS ++   
Sbjct: 83  -------------------------LVDARQLHKEAANEPELRQMAEEEIAHLSHRIAQL 142

Query: 122 EEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSS 181
           EE+LK++LLP DP D +NIMLE+RAG GGDEAGIWAGDLVRMY +Y++   W+ + VS S
Sbjct: 143 EERLKILLLPRDPNDEKNIMLEIRAGAGGDEAGIWAGDLVRMYSRYAKTQGWQVTLVSES 202

Query: 182 EAEKGGFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEV 241
            AE GGFK  +LEIKG RVYSKLKYESGVHRVQRVP TE  GR+HTSTATVA+MPE DEV
Sbjct: 203 LAEMGGFKEAILEIKGDRVYSKLKYESGVHRVQRVPATEASGRIHTSTATVAVMPEVDEV 262

Query: 242 EVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQL 301
           EV IDPKDIELTTARSGGAGGQNVNKVETA+DL+HKPTGIRIFCTEER+QL+NK RAFQ+
Sbjct: 263 EVEIDPKDIELTTARSGGAGGQNVNKVETAVDLYHKPTGIRIFCTEERSQLKNKERAFQI 322

Query: 302 LRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLE 361
           LRAKLYEIK+REQQE I + R+ QVGTG R+EKIRTYNYKD+RVTDHRL  NF L+S LE
Sbjct: 323 LRAKLYEIKLREQQEAITSMRRSQVGTGDRSEKIRTYNYKDDRVTDHRLGQNFSLSSVLE 360

Query: 362 GDIENAVQACVAMEQKELLEELA 379
           GD+E  +QAC++ +Q+E L +LA
Sbjct: 383 GDLEPIIQACISRDQQEQLAQLA 360

[Close]
BLAST of Lsi02G023660 vs. ExPASy Swiss-Prot
Match: B7KI17 (Peptide chain release factor 1 OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=prfA PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 7.9e-115
Identity = 224/379 (59.10%), Postives = 268/379 (70.71%), Query Frame = 0 

Query: 2   KLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFFL 61
           +LADPDI +NP E Q++A++ S L+E          N   T + T    +    ++    
Sbjct: 23  RLADPDIATNPDELQRVAKARSSLEET--------VNTYETWQKTKEEITGARQIL---- 82

Query: 62  PTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLKV 121
                                      KE+G D +M EM ALE+  L  QL+  E KLKV
Sbjct: 83  ---------------------------KEAGGDSEMREMAALEVEELEEQLEQLENKLKV 142

Query: 122 MLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKGG 181
           +LLP DP D +NIMLE+RAGTGGDEA IWAGDLVRMY +Y+E  +W    VS S A+ GG
Sbjct: 143 LLLPRDPNDDKNIMLEIRAGTGGDEASIWAGDLVRMYSRYAESQNWVVKLVSESLADMGG 202

Query: 182 FKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDP 241
           FK  +LEIKG +VYSKLK+E+GVHRVQRVP TE  GRVHTSTATVAIMPE DEVEV IDP
Sbjct: 203 FKEAILEIKGDQVYSKLKFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEVDEVEVQIDP 262

Query: 242 KDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLY 301
           KDIE++TARSGGAGGQNVNKVETA+DLFHKPTGIRIFCTEER+QLQN+ RA Q+LRAKLY
Sbjct: 263 KDIEVSTARSGGAGGQNVNKVETAVDLFHKPTGIRIFCTEERSQLQNRERAMQILRAKLY 322

Query: 302 EIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIENA 361
           E+K+REQQE + + R+ QVGTG+R+EKIRTYNYKDNRVTDHRL  NF L   LEGDIE  
Sbjct: 323 EMKLREQQEAVSSMRRSQVGTGSRSEKIRTYNYKDNRVTDHRLGQNFSLVGALEGDIEVI 362

Query: 362 VQACVAMEQKELLEELAQS 381
           +Q+C+  +Q+E L ELA S
Sbjct: 383 IQSCIGKDQQERLAELANS 362

[Close]
BLAST of Lsi02G023660 vs. ExPASy Swiss-Prot
Match: Q8YPK9 (Peptide chain release factor 1 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=prfA PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.3e-114
Identity = 220/382 (57.59%), Postives = 270/382 (70.68%), Query Frame = 0 

Query: 2   KLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFFL 61
           +L DPD   NP EYQK+A+S S L+EV +     Y+N                       
Sbjct: 23  RLGDPDTAKNPDEYQKIAKSRSSLEEVVE----TYDN----------------------- 82

Query: 62  PTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLKV 121
               W +  +         +I    + KE+ +D DM EM ALE+  L  ++   E +LKV
Sbjct: 83  ----WKIAQE--------ELIGARQVLKEANSDPDMHEMAALEVKELEEKIDYLETRLKV 142

Query: 122 MLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKGG 181
           +LLP DP D +NIMLE+RAGTGGDEA IWAGDL+RMY +Y++   W+   VS S  E GG
Sbjct: 143 LLLPRDPNDDKNIMLEIRAGTGGDEASIWAGDLLRMYSRYADTQGWRVKLVSESLGEMGG 202

Query: 182 FKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDP 241
           FK  +LEI+G+ VYSKLK+E+GVHRVQRVP TE  GRVHTSTATVAIMPE DEVE+ IDP
Sbjct: 203 FKEVILEIQGESVYSKLKFEAGVHRVQRVPATEAGGRVHTSTATVAIMPEVDEVEIHIDP 262

Query: 242 KDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLY 301
           KDIE+TTARSGGAGGQNVNKVETA+DL HKPTGIRIFCTEER+QLQNK RA Q+LRAKLY
Sbjct: 263 KDIEMTTARSGGAGGQNVNKVETAVDLMHKPTGIRIFCTEERSQLQNKERAMQILRAKLY 322

Query: 302 EIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIENA 361
           EIK+REQQE++ + R+ QVGTG+R+EKIRTYNYKD+R TDHRL  NF L   LEGD+E  
Sbjct: 323 EIKLREQQEEVTSMRRSQVGTGSRSEKIRTYNYKDSRATDHRLGQNFSLNPVLEGDLETV 365

Query: 362 VQACVAMEQKELLEELAQSAVA 384
           +Q+C++ +Q+E L ELA S+ A
Sbjct: 383 IQSCISQDQQERLAELATSSAA 365

[Close]
BLAST of Lsi02G023660 vs. ExPASy Swiss-Prot
Match: B7K205 (Peptide chain release factor 1 OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=prfA PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.3e-114
Identity = 221/379 (58.31%), Postives = 272/379 (71.77%), Query Frame = 0 

Query: 2   KLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFFL 61
           +LADPDI +NP E Q++A+  S L+E      L Y++                     + 
Sbjct: 23  RLADPDIATNPDELQRVARLRSSLEE----TVLTYDS---------------------WK 82

Query: 62  PTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLKV 121
            T   L+G                 + K++G D +M EM ALE+  L+ +L++ E+KLK+
Sbjct: 83  QTQEELIGA--------------KQILKDAGGDPEMREMAALEVEELAEKLEELEKKLKI 142

Query: 122 MLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKGG 181
           +LLP DP D +NIMLE+RAGTGGDEA IWAGDLVRMY +Y+E   W    VS S A+ GG
Sbjct: 143 LLLPRDPNDDKNIMLEIRAGTGGDEASIWAGDLVRMYSRYAESQKWTVKLVSESLADMGG 202

Query: 182 FKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDP 241
           FK  +LEI+G RVYSKLK+E+GVHRVQRVP TE  GRVHTSTATVAIMPE D+VEV ID 
Sbjct: 203 FKEAILEIQGDRVYSKLKFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEVDDVEVQIDA 262

Query: 242 KDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLY 301
           KDIE+TTARSGGAGGQNVNKVETA+DLF+KP GIRIFCTEER+QLQN+ RA Q+LRAKLY
Sbjct: 263 KDIEITTARSGGAGGQNVNKVETAVDLFYKPLGIRIFCTEERSQLQNRERAMQILRAKLY 322

Query: 302 EIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIENA 361
           ++K+REQQ+ + + RK QVGTGAR+EKIRTYNYKDNRVTDHRL  NF L   LEGDIE  
Sbjct: 323 DLKLREQQDAVSSMRKSQVGTGARSEKIRTYNYKDNRVTDHRLGQNFSLAGTLEGDIEEI 362

Query: 362 VQACVAMEQKELLEELAQS 381
           +Q+C++ +Q+E LEELA S
Sbjct: 383 IQSCISQDQQERLEELAAS 362

[Close]
BLAST of Lsi02G023660 vs. ExPASy TrEMBL
Match: A0A5A7VLD6 (Peptide chain release factor APG3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00120 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.3e-164
Identity = 323/385 (83.90%), Postives = 330/385 (85.71%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPD+VSNPTEYQKLAQSMSELDEV                      S+F      F
Sbjct: 70  VKLADPDVVSNPTEYQKLAQSMSELDEV---------------------VSTFRK----F 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
                 L                  ALSKESGNDEDMVEMIALEISSLSS+LKD EEKLK
Sbjct: 130 KECERQLEEA--------------KALSKESGNDEDMVEMIALEISSLSSELKDIEEKLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           G+KTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVE+VID
Sbjct: 250 GYKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEIVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL
Sbjct: 310 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLK NFELTSFL+GDIE+
Sbjct: 370 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLDGDIED 415

Query: 361 AVQACVAMEQKELLEELAQSAVASA 386
           AVQAC AMEQKELLEELAQSAVASA
Sbjct: 430 AVQACTAMEQKELLEELAQSAVASA 415

[Close]
BLAST of Lsi02G023660 vs. ExPASy TrEMBL
Match: A0A1S3BRZ7 (peptide chain release factor APG3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492855 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.3e-164
Identity = 323/385 (83.90%), Postives = 330/385 (85.71%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPD+VSNPTEYQKLAQSMSELDEV                      S+F      F
Sbjct: 70  VKLADPDVVSNPTEYQKLAQSMSELDEV---------------------VSTFRK----F 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
                 L                  ALSKESGNDEDMVEMIALEISSLSS+LKD EEKLK
Sbjct: 130 KECERQLEEA--------------KALSKESGNDEDMVEMIALEISSLSSELKDIEEKLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           G+KTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVE+VID
Sbjct: 250 GYKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEIVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL
Sbjct: 310 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLK NFELTSFL+GDIE+
Sbjct: 370 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLDGDIED 415

Query: 361 AVQACVAMEQKELLEELAQSAVASA 386
           AVQAC AMEQKELLEELAQSAVASA
Sbjct: 430 AVQACTAMEQKELLEELAQSAVASA 415

[Close]
BLAST of Lsi02G023660 vs. ExPASy TrEMBL
Match: A0A6J1DC40 (peptide chain release factor APG3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019348 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 6.9e-162
Identity = 320/385 (83.12%), Postives = 324/385 (84.16%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPDIVSNPTEYQK AQSMSELDEV                     F +F       
Sbjct: 70  VKLADPDIVSNPTEYQKFAQSMSELDEVVS------------------TFRNFKECEKQL 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
             T                      AL+KESGNDEDM EMIALEI+SLSSQLKD EEKLK
Sbjct: 130 EET---------------------KALAKESGNDEDMAEMIALEINSLSSQLKDLEEKLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSP+SSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           GFKTYVLEIKG  VYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID
Sbjct: 250 GFKTYVLEIKGNCVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIEL TARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL
Sbjct: 310 PKDIELQTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQE IRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN
Sbjct: 370 YEIKVREQQELIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 415

Query: 361 AVQACVAMEQKELLEELAQSAVASA 386
           AVQAC AMEQKELLEELAQSAVASA
Sbjct: 430 AVQACAAMEQKELLEELAQSAVASA 415

[Close]
BLAST of Lsi02G023660 vs. ExPASy TrEMBL
Match: A0A6J1KZW8 (peptide chain release factor APG3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111497823 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.2e-161
Identity = 315/383 (82.25%), Postives = 321/383 (83.81%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPDIVSNPTEYQKLAQSMSELDEV             T R                
Sbjct: 70  VKLADPDIVSNPTEYQKLAQSMSELDEV-----------VSTFRRFKECEKQLQE----- 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
                                    AL+KESGNDEDM EMIALEISSLSSQLKD EEKLK
Sbjct: 130 -----------------------AKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSP+SSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           GFKTYVLEIKG RVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID
Sbjct: 250 GFKTYVLEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELTT RSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL
Sbjct: 310 PKDIELTTTRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQE+IRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSF++GDIEN
Sbjct: 370 YEIKVREQQERIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIEN 413

Query: 361 AVQACVAMEQKELLEELAQSAVA 384
           AVQACVAMEQKEL+EEL QS  A
Sbjct: 430 AVQACVAMEQKELMEELVQSTGA 413

[Close]
BLAST of Lsi02G023660 vs. ExPASy TrEMBL
Match: A0A6J1H5U4 (peptide chain release factor APG3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460751 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.2e-161
Identity = 315/383 (82.25%), Postives = 321/383 (83.81%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPDIVSNPTEYQKLAQSMSELDEV             T R                
Sbjct: 70  VKLADPDIVSNPTEYQKLAQSMSELDEV-----------VSTFRRFKECEKQLQE----- 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
                                    AL+KESGNDEDM EMIALEISSLSSQLKD EEKLK
Sbjct: 130 -----------------------AKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSP+SSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           GFKTYVLEIKG RVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID
Sbjct: 250 GFKTYVLEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELTT RSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL
Sbjct: 310 PKDIELTTTRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQE+IRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSF++GDIEN
Sbjct: 370 YEIKVREQQERIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIEN 413

Query: 361 AVQACVAMEQKELLEELAQSAVA 384
           AVQACVAMEQKEL+EEL QS  A
Sbjct: 430 AVQACVAMEQKELMEELVQSTGA 413

[Close]
BLAST of Lsi02G023660 vs. NCBI nr
Match: XP_038876731.1 (peptide chain release factor APG3, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 594.3 bits (1531), Expect = 7.3e-166
Identity = 325/384 (84.64%), Postives = 327/384 (85.16%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPDIVSNPTEYQKLAQSMSELDE    V L +       +                
Sbjct: 70  VKLADPDIVSNPTEYQKLAQSMSELDE----VVLTFRKFKECEKQLE------------- 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
                                    ALSKESGNDEDMVEMIALEISSLSSQLKD EE LK
Sbjct: 130 ----------------------EAKALSKESGNDEDMVEMIALEISSLSSQLKDIEENLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID
Sbjct: 250 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL
Sbjct: 310 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN
Sbjct: 370 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 414

Query: 361 AVQACVAMEQKELLEELAQSAVAS 385
           AVQAC AMEQKELLEELAQSAVAS
Sbjct: 430 AVQACAAMEQKELLEELAQSAVAS 414

[Close]
BLAST of Lsi02G023660 vs. NCBI nr
Match: XP_008451621.1 (PREDICTED: peptide chain release factor APG3, chloroplastic [Cucumis melo] >KAA0068037.1 peptide chain release factor APG3 [Cucumis melo var. makuwa] >TYK18091.1 peptide chain release factor APG3 [Cucumis melo var. makuwa])

HSP 1 Score: 586.6 bits (1511), Expect = 1.5e-163
Identity = 323/385 (83.90%), Postives = 330/385 (85.71%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPD+VSNPTEYQKLAQSMSELDEV                      S+F      F
Sbjct: 70  VKLADPDVVSNPTEYQKLAQSMSELDEV---------------------VSTFRK----F 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
                 L                  ALSKESGNDEDMVEMIALEISSLSS+LKD EEKLK
Sbjct: 130 KECERQLEEA--------------KALSKESGNDEDMVEMIALEISSLSSELKDIEEKLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           G+KTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVE+VID
Sbjct: 250 GYKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEIVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL
Sbjct: 310 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLK NFELTSFL+GDIE+
Sbjct: 370 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLDGDIED 415

Query: 361 AVQACVAMEQKELLEELAQSAVASA 386
           AVQAC AMEQKELLEELAQSAVASA
Sbjct: 430 AVQACTAMEQKELLEELAQSAVASA 415

[Close]
BLAST of Lsi02G023660 vs. NCBI nr
Match: XP_031745234.1 (peptide chain release factor APG3, chloroplastic [Cucumis sativus] >KGN45452.2 hypothetical protein Csa_016721 [Cucumis sativus])

HSP 1 Score: 584.7 bits (1506), Expect = 5.8e-163
Identity = 325/385 (84.42%), Postives = 329/385 (85.45%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPDIVSNPTEYQKLAQSMSELDEV                      S+F      F
Sbjct: 70  VKLADPDIVSNPTEYQKLAQSMSELDEV---------------------VSTFRK----F 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
                 L                  ALSKESGNDEDMVEMIALEISSLSS+LKD EEKLK
Sbjct: 130 KECEKQLEEA--------------KALSKESGNDEDMVEMIALEISSLSSELKDIEEKLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           G+KTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID
Sbjct: 250 GYKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNK RAFQLLRAKL
Sbjct: 310 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLK NFELTSFLEGDIE+
Sbjct: 370 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLEGDIED 415

Query: 361 AVQACVAMEQKELLEELAQSAVASA 386
           AVQACV MEQKELLEELAQSAVASA
Sbjct: 430 AVQACVTMEQKELLEELAQSAVASA 415

[Close]
BLAST of Lsi02G023660 vs. NCBI nr
Match: XP_022151413.1 (peptide chain release factor APG3, chloroplastic [Momordica charantia])

HSP 1 Score: 580.1 bits (1494), Expect = 1.4e-161
Identity = 320/385 (83.12%), Postives = 324/385 (84.16%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPDIVSNPTEYQK AQSMSELDEV                     F +F       
Sbjct: 70  VKLADPDIVSNPTEYQKFAQSMSELDEVVS------------------TFRNFKECEKQL 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
             T                      AL+KESGNDEDM EMIALEI+SLSSQLKD EEKLK
Sbjct: 130 EET---------------------KALAKESGNDEDMAEMIALEINSLSSQLKDLEEKLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSP+SSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           GFKTYVLEIKG  VYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID
Sbjct: 250 GFKTYVLEIKGNCVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIEL TARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL
Sbjct: 310 PKDIELQTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQE IRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN
Sbjct: 370 YEIKVREQQELIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 415

Query: 361 AVQACVAMEQKELLEELAQSAVASA 386
           AVQAC AMEQKELLEELAQSAVASA
Sbjct: 430 AVQACAAMEQKELLEELAQSAVASA 415

[Close]
BLAST of Lsi02G023660 vs. NCBI nr
Match: XP_022959786.1 (peptide chain release factor APG3, chloroplastic [Cucurbita moschata] >XP_023004558.1 peptide chain release factor APG3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 579.3 bits (1492), Expect = 2.4e-161
Identity = 315/383 (82.25%), Postives = 321/383 (83.81%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPDIVSNPTEYQKLAQSMSELDEV             T R                
Sbjct: 70  VKLADPDIVSNPTEYQKLAQSMSELDEV-----------VSTFRRFKECEKQLQE----- 129

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
                                    AL+KESGNDEDM EMIALEISSLSSQLKD EEKLK
Sbjct: 130 -----------------------AKALAKESGNDEDMAEMIALEISSLSSQLKDIEEKLK 189

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSP+SSSEAEKG
Sbjct: 190 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKG 249

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           GFKTYVLEIKG RVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID
Sbjct: 250 GFKTYVLEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 309

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELTT RSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL
Sbjct: 310 PKDIELTTTRSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 369

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQE+IRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSF++GDIEN
Sbjct: 370 YEIKVREQQERIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFIDGDIEN 413

Query: 361 AVQACVAMEQKELLEELAQSAVA 384
           AVQACVAMEQKEL+EEL QS  A
Sbjct: 430 AVQACVAMEQKELMEELVQSTGA 413

[Close]
BLAST of Lsi02G023660 vs. TAIR 10
Match: AT3G62910.1 (Peptide chain release factor 1 )

HSP 1 Score: 510.4 bits (1313), Expect = 1.3e-144
Identity = 275/385 (71.43%), Postives = 312/385 (81.04%), Query Frame = 0 

Query: 1   VKLADPDIVSNPTEYQKLAQSMSELDEVWQDVCLQYNNLCGTVRMTSFIFSSFLPLICFF 60
           VKLADPD+VSN +EYQKLAQSMSELDEV                    +F  F       
Sbjct: 73  VKLADPDVVSNQSEYQKLAQSMSELDEV------------------VTVFRRFKDCEKQL 132

Query: 61  LPTYSWLVGVDWPDSFFFFVVIIMSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLK 120
           L +                       L+KE+G+DEDM EMI  EI+SL+ ++++ E++LK
Sbjct: 133 LES---------------------KVLAKEAGDDEDMAEMIGSEINSLTKEIEELEKQLK 192

Query: 121 VMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKG 180
           V+LLPSDPLDARNI+LEVRAGTGGDEA IW GDLVRMYQ+YSER+SWKFS VS SEAE G
Sbjct: 193 VLLLPSDPLDARNILLEVRAGTGGDEAAIWTGDLVRMYQRYSERSSWKFSMVSCSEAEHG 252

Query: 181 GFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 240
           G+KT V+EIKG RVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID
Sbjct: 253 GYKTCVMEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVID 312

Query: 241 PKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKL 300
           PKDIELT+ARSGGAGGQNVNKVETAIDLFHKP+GIRIFCTEERTQ++NK RAFQLLRAKL
Sbjct: 313 PKDIELTSARSGGAGGQNVNKVETAIDLFHKPSGIRIFCTEERTQIRNKARAFQLLRAKL 372

Query: 301 YEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKMNFELTSFLEGDIEN 360
           YEIKVREQQEKIRN+RK QVGTGAR+EKIRTYNYKD+RVTDHRLKMNF LT+FL+G +E+
Sbjct: 373 YEIKVREQQEKIRNERKSQVGTGARSEKIRTYNYKDSRVTDHRLKMNFALTTFLDGALED 418

Query: 361 AVQACVAMEQKELLEELAQSAVASA 386
           AVQAC A+EQKEL+EEL++S  AS+
Sbjct: 433 AVQACAALEQKELMEELSESVAASS 418

[Close]
BLAST of Lsi02G023660 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 225.7 bits (574), Expect = 6.3e-59
Identity = 115/261 (44.06%), Postives = 172/261 (65.90%), Query Frame = 0 

Query: 84  MSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLKVMLLPSDPLDARNIMLEVRAGTG 143
           + +L  ES +D+DM+++   E+     + K  +  L   LLP D  D R+ +LEVRAGTG
Sbjct: 114 LKSLVSESSDDKDMLDLAVGELDEAVEEEKRLQTLLLKSLLPKDEADERDCILEVRAGTG 173

Query: 144 GDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKGGFKTYVLEIKGQRVYSKLKYESG 203
           G+EA ++A D+ RMY++YS++  WKF  V  +E++  G+K     I G  VY KLK+ESG
Sbjct: 174 GEEASLFAMDIFRMYERYSQKKGWKFDIVDITESDMKGYKEASAAICGASVYGKLKFESG 233

Query: 204 VHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVE 263
           +HRVQR+P TE  GR+HTS  +VAI+P+ADEV+V +  +D+ + T RSGG+GGQ+ N   
Sbjct: 234 IHRVQRIPITEKSGRIHTSAISVAILPQADEVDVQLRNEDLRIDTYRSGGSGGQHANTTN 293

Query: 264 TAIDLFHKPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTG 323
           +A+ + H PTG+ +   +ER+Q  N+ +A ++L A+LYEI+    Q      R  Q+G+G
Sbjct: 294 SAVRIIHLPTGMMVSIQDERSQHMNRAKALKVLCARLYEIERLRIQSSRSKLRSDQIGSG 353

Query: 324 ARAEKIRTYNYKDNRVTDHRL 345
            R+ +IRTYN+   RVTDHR+
Sbjct: 354 DRSGRIRTYNFPQGRVTDHRV 374

[Close]
BLAST of Lsi02G023660 vs. TAIR 10
Match: AT2G47020.2 (Peptide chain release factor 1 )

HSP 1 Score: 185.7 bits (470), Expect = 7.3e-47
Identity = 90/202 (44.55%), Postives = 135/202 (66.83%), Query Frame = 0 

Query: 84  MSALSKESGNDEDMVEMIALEISSLSSQLKDTEEKLKVMLLPSDPLDARNIMLEVRAGTG 143
           + +L  ES +D+DM+++   E+     + K  +  L   LLP D  D R+ +LEVRAGTG
Sbjct: 49  LKSLVSESSDDKDMLDLAVGELDEAVEEEKRLQTLLLKSLLPKDEADERDCILEVRAGTG 108

Query: 144 GDEAGIWAGDLVRMYQKYSERNSWKFSPVSSSEAEKGGFKTYVLEIKGQRVYSKLKYESG 203
           G+EA ++A D+ RMY++YS++  WKF  V  +E++  G+K     I G  VY KLK+ESG
Sbjct: 109 GEEASLFAMDIFRMYERYSQKKGWKFDIVDITESDMKGYKEASAAICGASVYGKLKFESG 168

Query: 204 VHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVE 263
           +HRVQR+P TE  GR+HTS  +VAI+P+ADEV+V +  +D+ + T RSGG+GGQ+ N   
Sbjct: 169 IHRVQRIPITEKSGRIHTSAISVAILPQADEVDVQLRNEDLRIDTYRSGGSGGQHANTTN 228

Query: 264 TAIDLFHKPTGIRIFCTEERTQ 286
           +A+ + H PTG+ +   +ER+Q
Sbjct: 229 SAVRIIHLPTGMMVSIQDERSQ 250

[Close]
BLAST of Lsi02G023660 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 147.9 bits (372), Expect = 1.7e-35
Identity = 97/272 (35.66%), Postives = 150/272 (55.15%), Query Frame = 0 

Query: 104 EISSLSSQLKDTEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSE 163
           E   +  +L  + +K ++  L S P D    ++ + AG GG +A  WA  L+RMY ++ E
Sbjct: 177 EAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGE 236

Query: 164 RNSWKFSPVSSSEAEKGGFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTST 223
           +  +K   V  S  E+ G K+  LEI+G+  Y  +  E G HR+ R     ++G   TS 
Sbjct: 237 KQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSF 296

Query: 224 ATVAIMPEADE--VEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTE 283
           + V +MP   E  V + I  +D++++  R+GG GGQNVNKVETA+ + H PTG+ + CTE
Sbjct: 297 SGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTE 356

Query: 284 ERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAE---KIRTYNY---- 343
           ER+QL NK RA   L+AKL  I   ++  +I+  R    G   +AE   +IR Y +    
Sbjct: 357 ERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIR----GDAVKAEWGQQIRNYVFHPYK 416

Query: 344 --KDNRVTDHRLKMNFELTSFLEGDIENAVQA 365
             KD R T H      ++TS ++GD++  ++A
Sbjct: 417 LVKDVR-TGHETS---DITSVMDGDLDPFIKA 440

[Close]
BLAST of Lsi02G023660 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 147.9 bits (372), Expect = 1.7e-35
Identity = 97/272 (35.66%), Postives = 150/272 (55.15%), Query Frame = 0 

Query: 104 EISSLSSQLKDTEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSE 163
           E   +  +L  + +K ++  L S P D    ++ + AG GG +A  WA  L+RMY ++ E
Sbjct: 176 EAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGE 235

Query: 164 RNSWKFSPVSSSEAEKGGFKTYVLEIKGQRVYSKLKYESGVHRVQRVPQTETQGRVHTST 223
           +  +K   V  S  E+ G K+  LEI+G+  Y  +  E G HR+ R     ++G   TS 
Sbjct: 236 KQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSF 295

Query: 224 ATVAIMPEADE--VEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTE 283
           + V +MP   E  V + I  +D++++  R+GG GGQNVNKVETA+ + H PTG+ + CTE
Sbjct: 296 SGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTE 355

Query: 284 ERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAE---KIRTYNY---- 343
           ER+QL NK RA   L+AKL  I   ++  +I+  R    G   +AE   +IR Y +    
Sbjct: 356 ERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIR----GDAVKAEWGQQIRNYVFHPYK 415

Query: 344 --KDNRVTDHRLKMNFELTSFLEGDIENAVQA 365
             KD R T H      ++TS ++GD++  ++A
Sbjct: 416 LVKDVR-TGHETS---DITSVMDGDLDPFIKA 439

The following BLAST results are available for this feature:
BLAST of Lsi02G023660 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8RX791.8e-14371.43Peptide chain release factor APG3, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q8DMG93.2e-11658.22Peptide chain release factor 1 OS=Thermosynechococcus elongatus (strain BP-1) OX... [more]
B7KI177.9e-11559.10Peptide chain release factor 1 OS=Gloeothece citriformis (strain PCC 7424) OX=65... [more]
Q8YPK92.3e-11457.59Peptide chain release factor 1 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX... [more]
B7K2052.3e-11458.31Peptide chain release factor 1 OS=Rippkaea orientalis (strain PCC 8801) OX=41431... [more]
BLAST of Lsi02G023660 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7VLD67.3e-16483.90Peptide chain release factor APG3 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BRZ77.3e-16483.90peptide chain release factor APG3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1DC406.9e-16283.12peptide chain release factor APG3, chloroplastic OS=Momordica charantia OX=3673 ... [more]
A0A6J1KZW81.2e-16182.25peptide chain release factor APG3, chloroplastic OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1H5U41.2e-16182.25peptide chain release factor APG3, chloroplastic OS=Cucurbita moschata OX=3662 G... [more]
BLAST of Lsi02G023660 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038876731.17.3e-16684.64peptide chain release factor APG3, chloroplastic isoform X1 [Benincasa hispida][more]
XP_008451621.11.5e-16383.90PREDICTED: peptide chain release factor APG3, chloroplastic [Cucumis melo] >KAA0... [more]
XP_031745234.15.8e-16384.42peptide chain release factor APG3, chloroplastic [Cucumis sativus] >KGN45452.2 h... [more]
XP_022151413.11.4e-16183.12peptide chain release factor APG3, chloroplastic [Momordica charantia][more]
XP_022959786.12.4e-16182.25peptide chain release factor APG3, chloroplastic [Cucurbita moschata] >XP_023004... [more]
BLAST of Lsi02G023660 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G62910.11.3e-14471.43Peptide chain release factor 1 [more]
AT2G47020.16.3e-5944.06Peptide chain release factor 1 [more]
AT2G47020.27.3e-4744.55Peptide chain release factor 1 [more]
AT5G36170.11.7e-3535.66high chlorophyll fluorescent 109 [more]
AT5G36170.21.7e-3535.66high chlorophyll fluorescent 109 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 298..318
NoneNo IPR availableCOILSCoilCoilcoord: 98..118
NoneNo IPR availableGENE3D3.30.70.1660coord: 297..380
e-value: 1.6E-11
score: 46.2
NoneNo IPR availableGENE3D3.30.160.20coord: 241..296
e-value: 4.5E-24
score: 86.1
NoneNo IPR availableGENE3D3.30.70.1660coord: 130..240
e-value: 3.6E-46
score: 159.0
NoneNo IPR availableGENE3D6.10.140.1950coord: 84..128
e-value: 6.3E-7
score: 31.4
NoneNo IPR availablePANTHERPTHR43804LD18447Pcoord: 86..384
NoneNo IPR availablePANTHERPTHR43804:SF4PEPTIDE CHAIN RELEASE FACTOR APG3, CHLOROPLASTICcoord: 86..384
coord: 1..30
NoneNo IPR availablePANTHERPTHR43804LD18447Pcoord: 1..30
NoneNo IPR availableSUPERFAMILY75620Release factorcoord: 89..374
IPR005139Peptide chain release factorSMARTSM00937PCRF_a_2coord: 86..201
e-value: 2.8E-52
score: 189.7
IPR005139Peptide chain release factorPFAMPF03462PCRFcoord: 92..226
e-value: 6.4E-56
score: 189.2
IPR000352Peptide chain release factor class IPFAMPF00472RF-1coord: 233..343
e-value: 1.7E-35
score: 121.5
IPR000352Peptide chain release factor class IPROSITEPS00745RF_PROK_Icoord: 250..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G023660.1Lsi02G023660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0032544 plastid translation
biological_process GO:0010027 thylakoid membrane organization
biological_process GO:0006415 translational termination
cellular_component GO:0009507 chloroplast
molecular_function GO:0016149 translation release factor activity, codon specific
molecular_function GO:0003747 translation release factor activity