Lsi02G023560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G023560
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	E3 ubiquitin-protein ligase MIEL1-like
Location	chr02: 30224650 .. 30227343 (-)
RNA-Seq Expression	Lsi02G023560
Synteny	Lsi02G023560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TAACAATAATCATAACAAAAGATAAGGTAAGAAAAATGGTTGGTGGATGACAAGATGAGATTTCAATAAAATAATAATAAGAATATACCTAATAATAATATCAAAATCAAATGGGCAGCCAAAAACTAAATTATTTCAATCCATCTTCCAAATGGCTTCCATCAGATCCCTACTTTCCATAGCTCTCTTGGCTCTTCTCTTCATCTTCTCCGAATCACTACCCACTTCCAATTCTCTCACCGGAATTCCTCGCCGGAACATCTTTCAGACGGCAGCTCATGGGATTACAAATAGCAGTGTTGAGTCCTCTTCCCAAAGGAAGTCGAGTAATTTCCGTATCGAAGCTAGAAGGCGGATTCGACCTGTAAGTATCGGCAGCAGCCACCGTAAATCAGAGGCGAAACGGAGCCGAGGAGCATCCTATGAGGTTGGATCTGTGTTGGGTGTATGCATTTTCTTGAGATTGCTTTTGTGAATAATTTGTTTATGTAGGTCATGTTTGGTAATCATTTCGTTTTTTGTTTTTTATTTTGAAAAATTAAGTTTATTTTTTTTTATTTTCTTACCATGATTTTTATCAATCTTAAGTAGAAGAGTTGCATGATTAGTCAAATTTCAAAAATAAAAACAAGTTTTTAATTTTTTTTTTAGTTTTCAAATTTTGACTTGGTTTTTGAAAACATGGTAAAATGTTGATATCAAAGCAAAATATTTAGAGGTAGAAGAGGTGTTTATATGCTTAATTTGTAAAAACTAAAAATCAAAAACGAAATAGTTATCAAATGGGATCGTAAATTTTGGATTATGTTATATGTTCAAGGAATTTTGGTTTTTTTTTTTTTTTTTTTTTTTTGCGTGTGTATATTTAGCTGTAAGATCATTGAGGGGGAAAAAATGACTCTAAAATTTTGATTGCATCCAACTTAAATATAAGCATTATGATCTTAACTTAAATGAGTATCACATCCTAGCTCATAGTAAGATGATAAAAATAATGTCTCTATTTAACATTTACTAGAAAGAACAAAATTGAAAAATAATAAAATTATATCAAAATTCTTAAATAAAATATAAATAATAATTTTAAGTATCAACATAAGTACAATTTAACTAATCAATTTCGAAGTTGGAGGTTTGATTCTCCCAGTAAAAACAACAACAATGATGATAATTTATCAGTAATGATAATAATTTTGAATTATTGGTGATTTGTGTACATATCAAATTTGTGGTAATAATGGAGTTCCACCTCATGTTGACAATGAAAGGTTATATACCAATTTTTAGTTCAAATAAAAATATTAAAAACCAAACCATCTATATATCTATATACTATCGAAAAGAAGCTAGGAGAGAGAAACATTCGATTTCCCCTCTTACCTTTTTTTTAATGATTGATGTTATATAATATAATTTTACATTAAAATCATTAAAAAAAATTAAATCTAAATTTCTTTTATGAAGTACACTCTCAATTTTCTAAGCTTCCATTTTCTTCTCTCTTTATTTTTTAGTCATTTTTTTTTAATTTAACTTTTCCCCTTAGTCTAATATGTATTTATGAGTTTCTCTTTTGTTATTTGGTAAACTTATTTGTCAACATAAGTAGACAAAAAAGGAAAGGGCATATGAACAGAAAACTTCAATTCATATTTATTTTTATTTAATGTAATATTATTTATTATTCCTTTGTTACAGAGAGATTTTGTTTATTTTTCATTTAATATGTATATAATTTTTTTTTGTTATTCTCATGTTACATACACAATTTTGTATTTTATTAATTTATTTTATGCACCTCAAGATATTCTCTTCGAGTAGAAGATGAATGATTATAAATACAAATGAAAGTTTTTATTTTTTGAGATTTGATTTAAGATCAAGTCTAAATTTCATCTATTTTTCTTTTATGTGTATGTGTAGGGAAAGACATCATTTGATAATTCTCTACCATTATTCACTCATTAAGTACTCAAATTCAAATTTATTTTATTATATTTTATATTATAAGACTCTTATTTTAACTTATACATGTACATTTTATTTTCAACCTTATGGAACACGTGCAACGCACGTAGTTCCAACTAGTAGTATTAAAGTTAAAAATTTGAGAGTTTAAAATAAATAAAAAAAAATAAAAGAAAAATTACCTTTTTAGTCTCTTTGTTTTTAGGGGTAGGTGCATTTGGTCCATAAGTTTTCAATTTGGTCATTTTAGTCCTTCACTTTTAAAGAATAGTTTCCTATATTAATTAAATAATTTTTAAAAGAAATTTTAAATTAAAATTTAAAAGAAAATTTTCACCTTCATATTTATTTTATGTCGCCTTTTTTTTTTTTTCATTTTTCGTTTATATTCATCTGTTTCATCTTCCTTTCCTTCTTTAACTTATGTTTTTTTGTTTTAAAAAAATCAAGTCAAACAATGACAATGACATATAATTTCGATACCCATTTCAATTGTGATCCTTGAAATTTTATATTATCCTAAGCAACAATCAAATCGAATGGACAATTAAGATCAAAATCCCTCATGTCATCTTCCATTATATGCAACGATGAATACACTAGCCGCTCACTGAGGGCTTTGATTTGGCTTGGCGACTGCCTGACAATGACCGATCGAGTGACAGTATTGGAGCCGGAAGTGGAAGAATGGTGGAGAGGGAGGGGAGGAGAGGGGATCAAAAGTGA

mRNA sequence

TAACAATAATCATAACAAAAGATAAGGTAAGAAAAATGGTTGGTGGATGACAAGATGAGATTTCAATAAAATAATAATAAGAATATACCTAATAATAATATCAAAATCAAATGGGCAGCCAAAAACTAAATTATTTCAATCCATCTTCCAAATGGCTTCCATCAGATCCCTACTTTCCATAGCTCTCTTGGCTCTTCTCTTCATCTTCTCCGAATCACTACCCACTTCCAATTCTCTCACCGGAATTCCTCGCCGGAACATCTTTCAGACGGCAGCTCATGGGATTACAAATAGCAGTGTTGAGTCCTCTTCCCAAAGGAAGTCGAGTAATTTCCGTATCGAAGCTAGAAGGCGGATTCGACCTGTAAGTATCGGCAGCAGCCACCGTAAATCAGAGGCGAAACGGAGCCGAGGAGCATCCTATGAGCCGCTCACTGAGGGCTTTGATTTGGCTTGGCGACTGCCTGACAATGACCGATCGAGTGACAGTATTGGAGCCGGAAGTGGAAGAATGGTGGAGAGGGAGGGGAGGAGAGGGGATCAAAAGTGA

Coding sequence (CDS)

ATGGCTTCCATCAGATCCCTACTTTCCATAGCTCTCTTGGCTCTTCTCTTCATCTTCTCCGAATCACTACCCACTTCCAATTCTCTCACCGGAATTCCTCGCCGGAACATCTTTCAGACGGCAGCTCATGGGATTACAAATAGCAGTGTTGAGTCCTCTTCCCAAAGGAAGTCGAGTAATTTCCGTATCGAAGCTAGAAGGCGGATTCGACCTGTAAGTATCGGCAGCAGCCACCGTAAATCAGAGGCGAAACGGAGCCGAGGAGCATCCTATGAGCCGCTCACTGAGGGCTTTGATTTGGCTTGGCGACTGCCTGACAATGACCGATCGAGTGACAGTATTGGAGCCGGAAGTGGAAGAATGGTGGAGAGGGAGGGGAGGAGAGGGGATCAAAAGTGA

Protein sequence

MASIRSLLSIALLALLFIFSESLPTSNSLTGIPRRNIFQTAAHGITNSSVESSSQRKSSNFRIEARRRIRPVSIGSSHRKSEAKRSRGASYEPLTEGFDLAWRLPDNDRSSDSIGAGSGRMVEREGRRGDQK

Homology

[Close]

BLAST of Lsi02G023560 vs. ExPASy TrEMBL
Match: A0A5D3D3Y6 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001920 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 8.7e-16
Identity = 61/95 (64.21%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 1  MASIRSLLSIALLALLFIFSESLPTSNSLTGIPRRNIFQTAAHGITNSSVESSSQRKSSN 60
          MA+IR L+SIALLALLF FSESL TS+SLTGIP RNI  T AH I N SVESS QRKS+ 
Sbjct: 1  MAAIRPLVSIALLALLF-FSESLLTSHSLTGIPHRNILHTVAHRIVNGSVESSYQRKSNK 60

Query: 61 FRIEARRRIRPVSIGSS---HRKSEAKRSRGASYE 93
             + RRRIRP+ I  S   H KS   RSR AS+E
Sbjct: 61 LHTKDRRRIRPILISGSNGMHHKSAENRSRVASFE 94

[Close]

BLAST of Lsi02G023560 vs. ExPASy TrEMBL
Match: A0A1S3BT53 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo OX=3656 GN=LOC103492883 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 8.7e-16
Identity = 61/95 (64.21%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 1  MASIRSLLSIALLALLFIFSESLPTSNSLTGIPRRNIFQTAAHGITNSSVESSSQRKSSN 60
          MA+IR L+SIALLALLF FSESL TS+SLTGIP RNI  T AH I N SVESS QRKS+ 
Sbjct: 1  MAAIRPLVSIALLALLF-FSESLLTSHSLTGIPHRNILHTVAHRIVNGSVESSYQRKSNK 60

Query: 61 FRIEARRRIRPVSIGSS---HRKSEAKRSRGASYE 93
             + RRRIRP+ I  S   H KS   RSR AS+E
Sbjct: 61 LHTKDRRRIRPILISGSNGMHHKSAENRSRVASFE 94

[Close]

BLAST of Lsi02G023560 vs. ExPASy TrEMBL
Match: A0A0A0K929 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G394020 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 5.1e-08
Identity = 52/92 (56.52%), Postives = 60/92 (65.22%), Query Frame = 0


Query: 1  MASIRSLLSIALLALLFIFSESLPTSNSLTGIPRRNIFQTAAHGITNSSVESSSQ--RKS 60
          MA+IR LLSIA LA LF FSESL TS+SLTGIP R I  TAAH I + SVE SS   RKS
Sbjct: 1  MAAIRPLLSIAFLA-LFFFSESLLTSHSLTGIPHRTILNTAAHRIVDGSVEYSSYQIRKS 60

Query: 61 SNFRIE--ARRRIRPVSIGSS---HRKSEAKR 86
          +   I+   +RR  P+ + SS   H KS A R
Sbjct: 61 NKLHIKDIRKRRSGPILVSSSNGNHHKSAANR 91

[Close]

BLAST of Lsi02G023560 vs. ExPASy TrEMBL
Match: A0A5A7VLE5 (Protein no-on-transient A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00010 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.5e-04
Identity = 48/102 (47.06%), Postives = 62/102 (60.78%), Query Frame = 0


Query: 1  MASIRSLLSIALLALLFIFSESLPT--------SNSLTGIPRRNIFQTAAHGITNSSVES 60
          MA+I+SLL I+LLALLF FSESL T        SNS+TG+ RRN+ QTA H I +S  + 
Sbjct: 1  MAAIKSLLFISLLALLF-FSESLSTSAADCRGASNSVTGMLRRNVLQTATHEIADSEGKM 60

Query: 61 SSQRKSSNFRIEARRRIRPVSIG---SSHRKSEAKRSRGASY 92
          +   K          RIRPV+ G   + +RKS A RS+ AS+
Sbjct: 61 NVHIKRG-----GGGRIRPVAAGTGFTGNRKSAASRSQVASF 96

[Close]

BLAST of Lsi02G023560 vs. NCBI nr
Match: KAA0046715.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa] >TYK18251.1 E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 93.2 bits (230), Expect = 1.8e-15
Identity = 61/95 (64.21%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 1  MASIRSLLSIALLALLFIFSESLPTSNSLTGIPRRNIFQTAAHGITNSSVESSSQRKSSN 60
          MA+IR L+SIALLALLF FSESL TS+SLTGIP RNI  T AH I N SVESS QRKS+ 
Sbjct: 1  MAAIRPLVSIALLALLF-FSESLLTSHSLTGIPHRNILHTVAHRIVNGSVESSYQRKSNK 60

Query: 61 FRIEARRRIRPVSIGSS---HRKSEAKRSRGASYE 93
             + RRRIRP+ I  S   H KS   RSR AS+E
Sbjct: 61 LHTKDRRRIRPILISGSNGMHHKSAENRSRVASFE 94

[Close]

BLAST of Lsi02G023560 vs. NCBI nr
Match: XP_008451651.1 (PREDICTED: E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo])

HSP 1 Score: 93.2 bits (230), Expect = 1.8e-15
Identity = 61/95 (64.21%), Postives = 68/95 (71.58%), Query Frame = 0


Query: 1  MASIRSLLSIALLALLFIFSESLPTSNSLTGIPRRNIFQTAAHGITNSSVESSSQRKSSN 60
          MA+IR L+SIALLALLF FSESL TS+SLTGIP RNI  T AH I N SVESS QRKS+ 
Sbjct: 1  MAAIRPLVSIALLALLF-FSESLLTSHSLTGIPHRNILHTVAHRIVNGSVESSYQRKSNK 60

Query: 61 FRIEARRRIRPVSIGSS---HRKSEAKRSRGASYE 93
             + RRRIRP+ I  S   H KS   RSR AS+E
Sbjct: 61 LHTKDRRRIRPILISGSNGMHHKSAENRSRVASFE 94

[Close]

BLAST of Lsi02G023560 vs. NCBI nr
Match: KAG6575859.1 (hypothetical protein SDJN03_26498, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014393.1 hypothetical protein SDJN02_24570, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 71.6 bits (174), Expect = 5.6e-09
Identity = 52/98 (53.06%), Postives = 68/98 (69.39%), Query Frame = 0


Query: 1  MASIRSLLSIALLALLFIFSESLPT--------SNSLTGIPRRNIFQTAAHGITNSSVES 60
          MA+++SL+S+ALLALL +FSESL          SNSLTG+ RRN+ QTAAH I ++SVES
Sbjct: 1  MAALKSLVSVALLALL-LFSESLTASAVDRSGDSNSLTGMLRRNVLQTAAHQIADNSVES 60

Query: 61 SSQRKSSNFRIEARRRIRPVSIGSSHRKSEAKRSRGAS 91
          S +RK     +  +RR+RPV+ G+  R S A RSR AS
Sbjct: 61 SPERK---MNVHIKRRMRPVATGT--RSSAANRSRIAS 92

[Close]

BLAST of Lsi02G023560 vs. NCBI nr
Match: KAA0068047.1 (protein no-on-transient A-like [Cucumis melo var. makuwa] >TYK18081.1 protein no-on-transient A-like [Cucumis melo var. makuwa])

HSP 1 Score: 55.8 bits (133), Expect = 3.2e-04
Identity = 48/102 (47.06%), Postives = 62/102 (60.78%), Query Frame = 0


Query: 1  MASIRSLLSIALLALLFIFSESLPT--------SNSLTGIPRRNIFQTAAHGITNSSVES 60
          MA+I+SLL I+LLALLF FSESL T        SNS+TG+ RRN+ QTA H I +S  + 
Sbjct: 1  MAAIKSLLFISLLALLF-FSESLSTSAADCRGASNSVTGMLRRNVLQTATHEIADSEGKM 60

Query: 61 SSQRKSSNFRIEARRRIRPVSIG---SSHRKSEAKRSRGASY 92
          +   K          RIRPV+ G   + +RKS A RS+ AS+
Sbjct: 61 NVHIKRG-----GGGRIRPVAAGTGFTGNRKSAASRSQVASF 96

The following BLAST results are available for this feature:

BLAST of Lsi02G023560 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi02G023560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D3Y6	8.7e-16	64.21	E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BT53	8.7e-16	64.21	E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo OX=3656 GN=LOC103492883 P...	[more]
A0A0A0K929	5.1e-08	56.52	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G394020 PE=4 SV=1	[more]
A0A5A7VLE5	1.5e-04	47.06	Protein no-on-transient A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]

BLAST of Lsi02G023560 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0046715.1	1.8e-15	64.21	E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa] >TYK18251.1 E3...	[more]
XP_008451651.1	1.8e-15	64.21	PREDICTED: E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo]	[more]
KAG6575859.1	5.6e-09	53.06	hypothetical protein SDJN03_26498, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAA0068047.1	3.2e-04	47.06	protein no-on-transient A-like [Cucumis melo var. makuwa] >TYK18081.1 protein no...	[more]

BLAST of Lsi02G023560 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 60..89
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 103..132
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 45..59
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 45..91

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G023560.1	Lsi02G023560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding