Lsi02G023050 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G023050
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Adenylate isopentenyltransferase 3
Location	chr02: 29649959 .. 29650951 (+)
RNA-Seq Expression	Lsi02G023050
Synteny	Lsi02G023050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTGTCCACCATGTTCATGAGCAAACAGATCACTCAACAACCATTGATAAATCTTCCCCCAGGAAGACTAAACACCAACCCTTTCTTCAACTTTAGGAAGCTGCAAAAAGAGAAGGTGGTAGTCGTAATCGGCGCCACCGGAACTGGAAAATCTCGGCTGTCGATCGACATCGCCACCCAATTCCCATCGGAAGTCATAAACTCCGACAAAATGCAAGTCTACGATGGCCTCGACATAGTAACAAACAAAATCACAAAGCAAGAACAACGTGGCGTTTCTCATCACTTACTCGGAGTTATCAATCCCTTCTCCGATTTCTCTGCAACTAACTTCTCCGATATGGCTATGCTTTCCTTGGATGCCATTTCTAGCCATGATCGTCTCCCAATCATCGCCGGTGGCTCAAATTCTTATATTGAAGCCTTAATTGACTCGAAGTTTCGATCGAAATATGAATGTTGTTTCCTTTGGGTAGATGTGTCAATGCAAGTATTGCAATCCTTTGTATCCGAACGAGTAGACAAGATGGTAGAAAATGGAATGGTCGACGAGGCCAAAAAATTCTTCGATCCCGCCCGAGATTATTCTCGAGGGATCAAAAGGGCAATCGGAGTTCCGGAATTCGACTCGTATTTTCGATACAAATCATTGTTAGATGAGGAAGATCAAGAGATACTTTTGAAGGAATCCATATCAAAAGTGAAGGAGCATACATGTAAACTAGCATTGAAACAATTGGGAAAGATCCAACGGCTGAGAAATGTCAAAGGTTGGAACATACATAGATTGGATGCTACAGAGGCCTTTGCAAAGCAAGGGAAAGAAGCAGAGGAAGCATGGAACAAGCTCGTCGCCGTACCGAGCTCGACGATAATCGACCAGTTTCTTCACCCGAATGTCGTTGCCACAAAATTCCCGACCAATTTAGCATCCCTCATCAGCGTGCCGCGGAAGAGAAGTGCAGCCATGGCAGCTGCAACTCCTTGA

mRNA sequence

ATGAATGTGTCCACCATGTTCATGAGCAAACAGATCACTCAACAACCATTGATAAATCTTCCCCCAGGAAGACTAAACACCAACCCTTTCTTCAACTTTAGGAAGCTGCAAAAAGAGAAGGTGGTAGTCGTAATCGGCGCCACCGGAACTGGAAAATCTCGGCTGTCGATCGACATCGCCACCCAATTCCCATCGGAAGTCATAAACTCCGACAAAATGCAAGTCTACGATGGCCTCGACATAGTAACAAACAAAATCACAAAGCAAGAACAACGTGGCGTTTCTCATCACTTACTCGGAGTTATCAATCCCTTCTCCGATTTCTCTGCAACTAACTTCTCCGATATGGCTATGCTTTCCTTGGATGCCATTTCTAGCCATGATCGTCTCCCAATCATCGCCGGTGGCTCAAATTCTTATATTGAAGCCTTAATTGACTCGAAGTTTCGATCGAAATATGAATGTTGTTTCCTTTGGGTAGATGTGTCAATGCAAGTATTGCAATCCTTTGTATCCGAACGAGTAGACAAGATGGTAGAAAATGGAATGGTCGACGAGGCCAAAAAATTCTTCGATCCCGCCCGAGATTATTCTCGAGGGATCAAAAGGGCAATCGGAGTTCCGGAATTCGACTCGTATTTTCGATACAAATCATTGTTAGATGAGGAAGATCAAGAGATACTTTTGAAGGAATCCATATCAAAAGTGAAGGAGCATACATGTAAACTAGCATTGAAACAATTGGGAAAGATCCAACGGCTGAGAAATGTCAAAGGTTGGAACATACATAGATTGGATGCTACAGAGGCCTTTGCAAAGCAAGGGAAAGAAGCAGAGGAAGCATGGAACAAGCTCGTCGCCGTACCGAGCTCGACGATAATCGACCAGTTTCTTCACCCGAATGTCGTTGCCACAAAATTCCCGACCAATTTAGCATCCCTCATCAGCGTGCCGCGGAAGAGAAGTGCAGCCATGGCAGCTGCAACTCCTTGA

Coding sequence (CDS)

ATGAATGTGTCCACCATGTTCATGAGCAAACAGATCACTCAACAACCATTGATAAATCTTCCCCCAGGAAGACTAAACACCAACCCTTTCTTCAACTTTAGGAAGCTGCAAAAAGAGAAGGTGGTAGTCGTAATCGGCGCCACCGGAACTGGAAAATCTCGGCTGTCGATCGACATCGCCACCCAATTCCCATCGGAAGTCATAAACTCCGACAAAATGCAAGTCTACGATGGCCTCGACATAGTAACAAACAAAATCACAAAGCAAGAACAACGTGGCGTTTCTCATCACTTACTCGGAGTTATCAATCCCTTCTCCGATTTCTCTGCAACTAACTTCTCCGATATGGCTATGCTTTCCTTGGATGCCATTTCTAGCCATGATCGTCTCCCAATCATCGCCGGTGGCTCAAATTCTTATATTGAAGCCTTAATTGACTCGAAGTTTCGATCGAAATATGAATGTTGTTTCCTTTGGGTAGATGTGTCAATGCAAGTATTGCAATCCTTTGTATCCGAACGAGTAGACAAGATGGTAGAAAATGGAATGGTCGACGAGGCCAAAAAATTCTTCGATCCCGCCCGAGATTATTCTCGAGGGATCAAAAGGGCAATCGGAGTTCCGGAATTCGACTCGTATTTTCGATACAAATCATTGTTAGATGAGGAAGATCAAGAGATACTTTTGAAGGAATCCATATCAAAAGTGAAGGAGCATACATGTAAACTAGCATTGAAACAATTGGGAAAGATCCAACGGCTGAGAAATGTCAAAGGTTGGAACATACATAGATTGGATGCTACAGAGGCCTTTGCAAAGCAAGGGAAAGAAGCAGAGGAAGCATGGAACAAGCTCGTCGCCGTACCGAGCTCGACGATAATCGACCAGTTTCTTCACCCGAATGTCGTTGCCACAAAATTCCCGACCAATTTAGCATCCCTCATCAGCGTGCCGCGGAAGAGAAGTGCAGCCATGGCAGCTGCAACTCCTTGA

Protein sequence

MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHTCKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHPNVVATKFPTNLASLISVPRKRSAAMAAATP

Homology

[Close]

BLAST of Lsi02G023050 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 8.6e-86
Identity = 162/304 (53.29%), Postives = 220/304 (72.37%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N         K+KVVV++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+F +E+INSDK+QV+ GLDIVTNKIT +E  GV HHLLGV+ P +D +A N+  MA LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDAISSHDRLPIIAGGSNSYIEALID---SKFRSKYECCFLWVDVSMQVLQSFVSERVDK 180
           ++++ +  +LPII GGSNSY+EAL+D   +KFRS+Y+CCFLWVDV++ VL  FVSERVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVENGMVDEAKKFFDPAR-DYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKV 240
           MVE+GMV+E ++FFD +  DYSRGIK+AIG PEFD +FR +  L+ ED+E LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGK--EAEEAWNKLVAVPSSTII 299
           K +T +LA +Q  KI+RLR VK W+I R+DAT  F K+    +A  AW +LVA PS+  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

[Close]

BLAST of Lsi02G023050 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 306.6 bits (784), Expect = 3.4e-82
Identity = 161/289 (55.71%), Postives = 212/289 (73.36%), Query Frame = 0


Query: 15  QPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQ 74
           QPL     G +   PFF     +K+KVV V+GATGTGKSRL+ID+AT+FP+E++NSDK+Q
Sbjct: 13  QPLSLNFQGNMVDVPFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQ 72

Query: 75  VYDGLDIVTNKITKQEQRGVSHHLLGVI-NPFSDFSATNFSDMAMLSLDAISSHDRLPII 134
           VY GLDIVTNK+T +E  GV HHLLG + + + DF+A +F   A+ ++++I   DR+PII
Sbjct: 73  VYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPII 132

Query: 135 AGGSNSYIEALIDS--KFRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFF 194
           AGGSNSYIEAL++    FR +Y CCFLWVDVS  VL SFVSERVDKMV+ G+VDE ++ F
Sbjct: 133 AGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIF 192

Query: 195 DP-ARDYSRGIKRAIGVPEFDSYFRYKSL-LDEEDQEILLKESISKVKEHTCKLALKQLG 254
           DP + DYS GI+RAIGVPE D + R +      E  E LL+ +I K+KE+TC LA +QL 
Sbjct: 193 DPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQ 252

Query: 255 KIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFL 299
           KIQRL     WN+HR+DATE F ++G+EA+EAW+  VA PS+  +++FL
Sbjct: 253 KIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Lsi02G023050 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 273.5 bits (698), Expect = 3.2e-72
Identity = 139/283 (49.12%), Postives = 204/283 (72.08%), Query Frame = 0


Query: 24  RLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+AT+F  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKITKQEQRGVSHHLLGVI-NPFSDFSATNFSDMAMLSLDAISSHDRLPIIAGGSNSYIE 143
           NK+T +E RGV HHLLGV  +   + +AT +S +A  ++  +S++++LPI+AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 ALIDSK---FRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPARDYSR 203
           AL++       + Y+CCF+WVDVS+ VL SFVS+RVD+M+E G+++E ++ F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHTCKLALKQLGKIQRLRNVKG 263
           GI+RAIGVPE   Y R +SL+D   +  +L  ++  +K++T  LA +QL KIQRL     
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFAKQG-KEAEEAWNKLVAVPSSTIIDQFLHPN 302
            ++HR+DATE F K+  +E +EAW  LVA PS  I+D+F + N
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

[Close]

BLAST of Lsi02G023050 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.9e-61
Identity = 134/317 (42.27%), Postives = 193/317 (60.88%), Query Frame = 0


Query: 21  PPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYDGLD 80
           P     TN     ++ +KEK++V++GATGTGKSRLSID+A  FP EVINSDKMQVY GLD
Sbjct: 12  PTTTTTTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLD 71

Query: 81  IVTNKITKQEQRGVSHHLLGVINPF-SDFSATNFSDMAMLSLDAISSHDRLPIIAGGSNS 140
           I TNKI+  ++ GV HHLLG ++P   + +  +F  +A  ++  I+   +LP++ GGSNS
Sbjct: 72  ITTNKISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNS 131

Query: 141 YIEALIDSKFRS------------------KYECCFLWVDVSMQVLQSFVSERVDKMVEN 200
           +I AL+  +F S                  +Y+CCFLWVDVS++VL  ++++RVD M+E 
Sbjct: 132 FIHALLVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLEL 191

Query: 201 GMVDEAKKFFDPA-RDYSR------GIKRAIGVPEFDSYFRYKSLLDEEDQEI------- 260
           GM DE  +F+ P   D+        G+++AIGVPEFD YF      D E ++        
Sbjct: 192 GMFDELAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRR 251

Query: 261 -LLKESISKVKEHTCKLALKQLGKIQRLRNVKGWNIHRLDATEAF-----AKQGKEAEEA 299
              +E++  +KE+TC LA +Q+GKI RL+   GW++ RLDATE+F     +  G++  E 
Sbjct: 252 GAFEEAVRAIKENTCHLAKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEI 311

[Close]

BLAST of Lsi02G023050 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 233.4 bits (594), Expect = 3.7e-60
Identity = 134/336 (39.88%), Postives = 200/336 (59.52%), Query Frame = 0


Query: 4   STMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQ-------KEKVVVVIGATGTGKSRLS 63
           S  F S   +   L++    R    P  +  +++       K+KVVV++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINP-FSDFSATNFSD 123
           +D+AT+FPSE+INSDK+QVY+GL+I TN+IT Q++RGV HHLLGVINP   + +A  F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 MAMLSLDAISSHDRLPIIAGGSNSYIEALIDSKFRSK----------------YECCFLW 183
            A   +  I+S  ++PIIAGGSNS++ AL+  +F  K                YECCF+W
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPAR---DYSRGIKRAIGVPEFDSYFR- 243
           VDVS  VL  ++  RVD+M+++GM +E  +F+DP +   +   GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YKSLLDEEDQEILLKESISKVKEHTCKLALKQLGKIQRLRNVKGWNIHRLDATEAF 302
                K +  +  ++    +++  +K +T  LA +Q+ KI+ L++  GW I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDA-GWEIERVDATASF 322

[Close]

BLAST of Lsi02G023050 vs. ExPASy TrEMBL
Match: A0A5D3DBM7 (Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00080 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.7e-164
Identity = 299/330 (90.61%), Postives = 311/330 (94.24%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN FFN RKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFFNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR KYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFR +SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLALKQLGKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV +PS+TIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           N VATKFPTNLASLI VPRKRSAAMAAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

[Close]

BLAST of Lsi02G023050 vs. ExPASy TrEMBL
Match: A0A1S3BRC2 (adenylate isopentenyltransferase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492689 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.8e-164
Identity = 299/330 (90.61%), Postives = 310/330 (93.94%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN F N RKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFSNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           TQFPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR KYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFR +SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLALKQLGKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV +PS+TIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           N VATKFPTNLASLI VPRKRSAAMAAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

[Close]

BLAST of Lsi02G023050 vs. ExPASy TrEMBL
Match: A0A0A0K919 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 5.9e-162
Identity = 293/330 (88.79%), Postives = 309/330 (93.64%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMSK  TQQPLINLPPGRL TNPF   RKLQKEKVV+VIG TGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSKITTQQPLINLPPGRLTTNPFSTLRKLQKEKVVIVIGPTGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR +YECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFRY+SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLAL+QLGKIQRLRNVKGWNIHRLDATEAF KQGKEA+EAWNK V +PS+TIIDQFLH 
Sbjct: 241 CKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQGKEADEAWNKHVTIPSTTIIDQFLHQ 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ATKFPTNLASLI VPRK+SAAMAAATP
Sbjct: 301 NVIATKFPTNLASLIGVPRKKSAAMAAATP 330

[Close]

BLAST of Lsi02G023050 vs. ExPASy TrEMBL
Match: A0A6J1KSJ2 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497849 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.3e-156
Identity = 279/330 (84.55%), Postives = 306/330 (92.73%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN RKL+KEKVV+VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           TQFPSEVINSDKMQVYDGLDIVTNK+TKQEQRGV HHLLG+I+PFSDFSATNFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LD I   DRLPIIAGGSNSYIEALIDS F+SKYECCFLWVDVSM +L SFVS+RVDKMV 
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMV+EAK+FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+E QE LL+E+ISKVK++T
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLA +Q+GKIQRLRN+KGW IHRLDATEAF KQGKEAEE WNKLV VPS+TII+QFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ T  PTNLASL+ +PRKRSAA+AAATP
Sbjct: 301 NVITTNVPTNLASLLGLPRKRSAAIAAATP 330

[Close]

BLAST of Lsi02G023050 vs. ExPASy TrEMBL
Match: A0A6J1H8C9 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460993 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.6e-154
Identity = 276/330 (83.64%), Postives = 304/330 (92.12%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN RKL+KEKVV+VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVYDGLDIVTNK+TKQEQRGV HHLLG+I+PFSDFSATNFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           L  I   DRLPIIAGGSNSY+EALIDS F+SKYECCFLWVDVSM VL SFVS+RVDKMV 
Sbjct: 121 LVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA++FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+E QE LL+E+ISKVK++T
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLA +Q+GKIQRLRN+KGW IHRLDATEAF KQGKEAEE WNKLV VPS+TIIDQFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ TK PTNLA+L+ + R RSAA+AAATP
Sbjct: 301 NVITTKVPTNLATLLDLSRMRSAAIAAATP 330

[Close]

BLAST of Lsi02G023050 vs. NCBI nr
Match: XP_038899604.1 (adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 605.9 bits (1561), Expect = 2.1e-169
Identity = 305/330 (92.42%), Postives = 317/330 (96.06%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           M+VSTMFMSKQITQQPLINLPPGRLNTNPFFN RK QKEKVV+VIGATGTGKSRLS+DIA
Sbjct: 1   MDVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKPQKEKVVIVIGATGTGKSRLSVDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQRGV HHLLG+INPFSDFSATNFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYRGLDIVTNKITKQEQRGVPHHLLGIINPFSDFSATNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAI+SHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAITSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDP RDYSRGIKRAIGVPEFDSYFRYK LLDEE Q+ LLKESISKVKEHT
Sbjct: 181 NGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKPLLDEEAQDKLLKESISKVKEHT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLAL+QLGKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV VPS+T+IDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTVPSTTLIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NVVATKFPTNLASLI V RKRSAAMAAATP
Sbjct: 301 NVVATKFPTNLASLIGVSRKRSAAMAAATP 330

[Close]

BLAST of Lsi02G023050 vs. NCBI nr
Match: KAA0057613.1 (adenylate isopentenyltransferase 3 [Cucumis melo var. makuwa] >TYK20984.1 adenylate isopentenyltransferase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 588.6 bits (1516), Expect = 3.4e-164
Identity = 299/330 (90.61%), Postives = 311/330 (94.24%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN FFN RKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFFNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR KYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFR +SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLALKQLGKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV +PS+TIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           N VATKFPTNLASLI VPRKRSAAMAAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

[Close]

BLAST of Lsi02G023050 vs. NCBI nr
Match: XP_008451382.1 (PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Cucumis melo])

HSP 1 Score: 587.0 bits (1512), Expect = 9.9e-164
Identity = 299/330 (90.61%), Postives = 310/330 (93.94%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN F N RKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFSNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           TQFPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR KYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFR +SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLALKQLGKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV +PS+TIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           N VATKFPTNLASLI VPRKRSAAMAAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

[Close]

BLAST of Lsi02G023050 vs. NCBI nr
Match: XP_004136062.1 (adenylate isopentenyltransferase 3, chloroplastic [Cucumis sativus] >KGN44872.1 hypothetical protein Csa_015949 [Cucumis sativus])

HSP 1 Score: 580.1 bits (1494), Expect = 1.2e-161
Identity = 293/330 (88.79%), Postives = 309/330 (93.64%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMSK  TQQPLINLPPGRL TNPF   RKLQKEKVV+VIG TGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSKITTQQPLINLPPGRLTTNPFSTLRKLQKEKVVIVIGPTGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR +YECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFRY+SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLAL+QLGKIQRLRNVKGWNIHRLDATEAF KQGKEA+EAWNK V +PS+TIIDQFLH 
Sbjct: 241 CKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQGKEADEAWNKHVTIPSTTIIDQFLHQ 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ATKFPTNLASLI VPRK+SAAMAAATP
Sbjct: 301 NVIATKFPTNLASLIGVPRKKSAAMAAATP 330

[Close]

BLAST of Lsi02G023050 vs. NCBI nr
Match: XP_023004596.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 562.4 bits (1448), Expect = 2.6e-156
Identity = 279/330 (84.55%), Postives = 306/330 (92.73%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN RKL+KEKVV+VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           TQFPSEVINSDKMQVYDGLDIVTNK+TKQEQRGV HHLLG+I+PFSDFSATNFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LD I   DRLPIIAGGSNSYIEALIDS F+SKYECCFLWVDVSM +L SFVS+RVDKMV 
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMV+EAK+FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+E QE LL+E+ISKVK++T
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLA +Q+GKIQRLRN+KGW IHRLDATEAF KQGKEAEE WNKLV VPS+TII+QFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ T  PTNLASL+ +PRKRSAA+AAATP
Sbjct: 301 NVITTNVPTNLASLLGLPRKRSAAIAAATP 330

[Close]

BLAST of Lsi02G023050 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 318.5 bits (815), Expect = 6.1e-87
Identity = 162/304 (53.29%), Postives = 220/304 (72.37%), Query Frame = 0


Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N         K+KVVV++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+F +E+INSDK+QV+ GLDIVTNKIT +E  GV HHLLGV+ P +D +A N+  MA LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDAISSHDRLPIIAGGSNSYIEALID---SKFRSKYECCFLWVDVSMQVLQSFVSERVDK 180
           ++++ +  +LPII GGSNSY+EAL+D   +KFRS+Y+CCFLWVDV++ VL  FVSERVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVENGMVDEAKKFFDPAR-DYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKV 240
           MVE+GMV+E ++FFD +  DYSRGIK+AIG PEFD +FR +  L+ ED+E LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALKQLGKIQRLRNVKGWNIHRLDATEAFAKQGK--EAEEAWNKLVAVPSSTII 299
           K +T +LA +Q  KI+RLR VK W+I R+DAT  F K+    +A  AW +LVA PS+  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

[Close]

BLAST of Lsi02G023050 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 306.6 bits (784), Expect = 2.4e-83
Identity = 161/289 (55.71%), Postives = 212/289 (73.36%), Query Frame = 0


Query: 15  QPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQ 74
           QPL     G +   PFF     +K+KVV V+GATGTGKSRL+ID+AT+FP+E++NSDK+Q
Sbjct: 13  QPLSLNFQGNMVDVPFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQ 72

Query: 75  VYDGLDIVTNKITKQEQRGVSHHLLGVI-NPFSDFSATNFSDMAMLSLDAISSHDRLPII 134
           VY GLDIVTNK+T +E  GV HHLLG + + + DF+A +F   A+ ++++I   DR+PII
Sbjct: 73  VYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPII 132

Query: 135 AGGSNSYIEALIDS--KFRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFF 194
           AGGSNSYIEAL++    FR +Y CCFLWVDVS  VL SFVSERVDKMV+ G+VDE ++ F
Sbjct: 133 AGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIF 192

Query: 195 DP-ARDYSRGIKRAIGVPEFDSYFRYKSL-LDEEDQEILLKESISKVKEHTCKLALKQLG 254
           DP + DYS GI+RAIGVPE D + R +      E  E LL+ +I K+KE+TC LA +QL 
Sbjct: 193 DPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQ 252

Query: 255 KIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFL 299
           KIQRL     WN+HR+DATE F ++G+EA+EAW+  VA PS+  +++FL
Sbjct: 253 KIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Lsi02G023050 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 273.5 bits (698), Expect = 2.3e-73
Identity = 139/283 (49.12%), Postives = 204/283 (72.08%), Query Frame = 0


Query: 24  RLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+AT+F  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKITKQEQRGVSHHLLGVI-NPFSDFSATNFSDMAMLSLDAISSHDRLPIIAGGSNSYIE 143
           NK+T +E RGV HHLLGV  +   + +AT +S +A  ++  +S++++LPI+AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 ALIDSK---FRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPARDYSR 203
           AL++       + Y+CCF+WVDVS+ VL SFVS+RVD+M+E G+++E ++ F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHTCKLALKQLGKIQRLRNVKG 263
           GI+RAIGVPE   Y R +SL+D   +  +L  ++  +K++T  LA +QL KIQRL     
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFAKQG-KEAEEAWNKLVAVPSSTIIDQFLHPN 302
            ++HR+DATE F K+  +E +EAW  LVA PS  I+D+F + N
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

[Close]

BLAST of Lsi02G023050 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 233.4 bits (594), Expect = 2.6e-61
Identity = 134/336 (39.88%), Postives = 200/336 (59.52%), Query Frame = 0


Query: 4   STMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQ-------KEKVVVVIGATGTGKSRLS 63
           S  F S   +   L++    R    P  +  +++       K+KVVV++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINP-FSDFSATNFSD 123
           +D+AT+FPSE+INSDK+QVY+GL+I TN+IT Q++RGV HHLLGVINP   + +A  F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 MAMLSLDAISSHDRLPIIAGGSNSYIEALIDSKFRSK----------------YECCFLW 183
            A   +  I+S  ++PIIAGGSNS++ AL+  +F  K                YECCF+W
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPAR---DYSRGIKRAIGVPEFDSYFR- 243
           VDVS  VL  ++  RVD+M+++GM +E  +F+DP +   +   GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YKSLLDEEDQEILLKESISKVKEHTCKLALKQLGKIQRLRNVKGWNIHRLDATEAF 302
                K +  +  ++    +++  +K +T  LA +Q+ KI+ L++  GW I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDA-GWEIERVDATASF 322

[Close]

BLAST of Lsi02G023050 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 226.9 bits (577), Expect = 2.4e-59
Identity = 135/328 (41.16%), Postives = 200/328 (60.98%), Query Frame = 0


Query: 2   NVSTMFMSKQI--TQQPLINLPPGRLNTNPFFN--FRKLQKEKVVVVIGATGTGKSRLSI 61
           N+++ F+S  +     P + LPP R +  P       +  K+KVVV++GATG+GKS LSI
Sbjct: 3   NLTSTFVSPSMIPITSPRLRLPPPR-SVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSI 62

Query: 62  DIATQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINP-FSDFSATNFSDM 121
           D+AT+F  E++NSDK+Q YDGL + TN+++  E+ GV HHLLG + P  S+ + + F  +
Sbjct: 63  DLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSL 122

Query: 122 AMLSLDAISSHDRLPIIAGGSNSYIEALIDSKFRSK---------------YECCFLWVD 181
           A  S+  I++   LPIIAGGSNS+I AL+  +F  K               YECCFLWVD
Sbjct: 123 ASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVD 182

Query: 182 VSMQVLQSFVSERVDKMVENGMVDEAKKFFDPARDYS----RGIKRAIGVPEFDSYF--- 241
           VS+ VL  ++S+RVD+M+E+GM +E   F+DP    S     GI + IG+PEFD YF   
Sbjct: 183 VSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLY 242

Query: 242 --RYKSLLDEEDQ--EILLKESISKVKEHTCKLALKQLGKIQRLRNVKGWNIHRLDATEA 299
               K  + E DQ  +    E++ ++KE+T +LA KQ+ +I +L++  GW+I RLDAT +
Sbjct: 243 PPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKLKS-SGWDIQRLDATPS 302

The following BLAST results are available for this feature:

BLAST of Lsi02G023050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WC9	8.6e-86	53.29	Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID2	3.4e-82	55.71	Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID1	3.2e-72	49.12	Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q5GHF7	1.9e-61	42.27	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]
Q94ID3	3.7e-60	39.88	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Lsi02G023050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DBM7	1.7e-164	90.61	Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BRC2	4.8e-164	90.61	adenylate isopentenyltransferase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A0A0K919	5.9e-162	88.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1	[more]
A0A6J1KSJ2	1.3e-156	84.55	adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1H8C9	2.6e-154	83.64	adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=...	[more]

BLAST of Lsi02G023050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899604.1	2.1e-169	92.42	adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida]	[more]
KAA0057613.1	3.4e-164	90.61	adenylate isopentenyltransferase 3 [Cucumis melo var. makuwa] >TYK20984.1 adenyl...	[more]
XP_008451382.1	9.9e-164	90.61	PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Cucumis melo]	[more]
XP_004136062.1	1.2e-161	88.79	adenylate isopentenyltransferase 3, chloroplastic [Cucumis sativus] >KGN44872.1 ...	[more]
XP_023004596.1	2.6e-156	84.55	adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Lsi02G023050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63110.1	6.1e-87	53.29	isopentenyltransferase 3	[more]
AT5G19040.1	2.4e-83	55.71	isopentenyltransferase 5	[more]
AT3G23630.1	2.3e-73	49.12	isopentenyltransferase 7	[more]
AT1G68460.1	2.6e-61	39.88	isopentenyltransferase 1	[more]
AT3G19160.1	2.4e-59	41.16	ATP/ADP isopentenyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 38..298 e-value: 1.4E-106 score: 357.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 37..191
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 164..262 e-value: 1.4E-106 score: 357.6
None	No IPR available	PFAM	PF01715	IPPT	coord: 151..248 e-value: 1.4E-11 score: 44.6 coord: 74..147 e-value: 4.5E-20 score: 72.4
None	No IPR available	PANTHER	PTHR11088:SF74	ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTIC	coord: 33..301
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 33..301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G023050.1	Lsi02G023050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009691	cytokinin biosynthetic process
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005634	nucleus
cellular_component	GO:0009536	plastid
molecular_function	GO:0009824	AMP dimethylallyltransferase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity