Lsi02G022720 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G022720
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGuanine nucleotide-binding protein
Locationchr02: 29308403 .. 29312875 (-)
RNA-Seq ExpressionLsi02G022720
SyntenyLsi02G022720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCCGGCTTTCTTTATTTCTCTTAAACCCTATTTCACTTTCAGCTACCCCATCCCTCCCAGCCACTTCACCCCGCCCCGCCGCCGTCTAACCGGCCTTCGCCGGACGCTGCACTCTCAGCTGCCACGGTCTCAGCCTACTGAAGAAGTTGCTTCTGCTCTGTGCCTCTCACAGTCTCTGGCCTCTCCGAGCAAACGGAGATATTAAGTTCCTTATCTCTGTTCCCCCTTAAAAAACACAGTGGTTCAGCCCTTGGCCCGTGCCGTTGGTGAGATCCCGCCGCCGCTCATTTTCAGATCCCCAGGTTTATTTTTCGTTCTATCTTGTTAGTTGGTTTTTGTTGGTTTATTCTCTTCACCCATGGAGAGAAATCAAGAAGAATTTTTTGAAATTTCTTCCTCAACCAGTCTAAATGAAACATAAAACTAAGAACATATGCATCACAGATTAGGAAAAAGAAAGATTGAATCCATACATAAAAATAAAATAAAGAACATATTATTCTTATCGTAATCAATAAATAAAAAGAAAAAACTCACCTGTATTTTGATTGGCTATAAAAACTTACAAATATTGTATTTGAAAATATTGAGATCATTGTTATCAGTTTAAAAAACGTCACAAGGGCAAGAAGAATAGGAAGTTATCCTTGAGTTTGAATTTGATCGAGTGTTGTTGAGTTTGCTTGAACATAGAAAAAATATATTGATGCAGATGAACTTTTGATTAGGTATATTTTCAAAATTTTCATGCTGGATTTTCTGTCTTTTGCTTGTGATACTGTATTTTTCGAAAGAACATTTTTGTGAGTCATTCACTTGTGTTGACAGTGAAGGCCTCTTGCACAAAGCCCTTGTCTTCTGAGACTCAAAGATGGTGAAGAGGAGCAAAAGTAAGTGGGTTCTATTTATAATATTGGAAGCCAACGCATTTTCTTTTGTTTTCTGCGGCTTCTGGGACACACTTGGTTTTATTCTCTAAAGTGTTTTGTTTTTTGTAGAGAGTAAAAGCAAGAGGGTTAGTTTAAAGAAGAAGTACAAGATCATTAGAAAGGTGAAGGAGCATCACAAGAAGAAGGCGAAGGAAGCCAAGAAGCTAAGCTTCAAGGGGAAGAGCAAAGTTGAGAAGGACCCGGGTATTCCCAATGATTGGCCCTTCAAGGAACAGGAGCTCAAGGCACTTGAAGCTCGTCGTGCCCGTGCTCTTGATGAGATGGAACAAAAGAAAGCAGCTCGGAAGGAGAGGGTATTATAATTTAATACTTCTAATCATGAACATTTTTAGATTTTTAATGTATGTATATATTTATGAGCCTGTGAATGGTGTATGTATTTTGGTACGCCATGTATATTACATCACTGTATAGCAATTTACTGGAAATCTCACTTTTTAATCTTGTTCAGGCTCAAAAGAGGAAGTTGGGGTTGCTAGATGATGATAATATGGCTTCTGCAGATCAAAGTTTACGAGAGATCAAGGATGGAGATAACTCTGTAGGGCCTGCCCGGAATCGTGGTAGGTTGTTTTCTATGAAGAGTTTTGGATAAAAATGGAGTAAACAAATTGTGATATTCATGTTCATTTCGTTGCATTGAGTTTTTGACTATAAATACGCAGATAACTCAGACAGGGTTTTCTACAAGGAATTGGTCAAAGTTATTGAGGCATCAGATGTTATTTTAGAAGTTTTGGATGCACGAGATCCCCTTGGCACCCGTTGTATTGATATGGAGAAGATGGTGATGAAAGCGGGTCCTAATAAGCATCTTGTACTACTTCTGAATAAAATTGGTAAACTTTTCCCCTTTGTGTCAAAATTCTACTAACTAATGAAGAAGGATATGAACATAGAAAATAGGTGCTATACATCGCGCTTAACTTTTACACTTTATTATAAATGATTTCAATGTTTTTCTTTAATATTTCATTATTTGTTCCTAATATATGTATATCTTTGCTTCGTGTTTGACTTGACTGGGCATGTTATGTCGTGTCGAAAACCAAAGAAAGGTTGTAAAACTAAGTTTTGTGTTTGATGAGTGCTTCTTTGTCAAGTGTAAGTTAACCAAGTTAAATGTTTGTGCTTTTTGGTAACTCACTACTGTTGTTGGTGGTTATTAGTTTGAACTTGATATGCTCTTAATCAGATGATCCTAGTGACCAGGTATTTCTACTTAGATTCCTCTTTAAGTTGTGGATGGGTTAGTCATGTGAAGCCCAAGAAACTGTGTATTTATCTTCATCTGCAGCAATTACAATCTGTATATGTATTTATATGTTTTTCATAACAGATCTTGTCCCTCGAGAGGCTGTTGAGAAGTGGCTTACTTATCTGAGAGAAGAATTGCCTGCTGTTGCCTTCAAGTGTAGCACACAAGAACAAAGGTCCAACTTAGGTTGGAAATCTTCAAAGACATCCAAAACGAAAACAAGCAATTTATTGCAGAGAAGTGACTGCCTTGGAGCTGAAACTCTAATTAAACTGTTGAAGAATTACTCAAGAAGTTATGAGGTACTTCTAAGGTTTTGGGAGTTTTATCAAAAACTTTTGTACTTTCAACTTTAAAGAATTGACCATTCTGTTTTGTTTTATTTGGTGTTGCCTTTTGTGCATTTGAAAGCTTGTGATGTGCTTAATCATCCCACTCAGGTTTTGATGATTATTTTTGGATATATACTGACCGTATATCCTTCTGCTCCATCGGATTAGTTCTCTAGCCTGCATTTTTTTGTTCTTTAAAGTTGTCTCTGATCAATATCTGGAATTTTCCTTTTCAATTTTGGTTAATTGACGTGGTCTGTTGTGTATTTGAATCTGACAGATTAAAAAATCAATAACCGTGGGTGTTATTGGCTTGCCTAATGTTGGCAAAAGCAGTCTTATCAATAGTCTGAAGAGATCCCACGTTGTCAACGTGGGTGCTACACCAGGATTAACAAGATCAATGCAAGAGGTCCATTTAGACAAGAACGTCAAATTGTTGGATTGCCCTGGTGTTGTGATGGTAAGATCTAAAGAAAACGAGCCATCTATAGCTTTGCGGAATTGCAAAAGAATTGAGAAGTTGGAAGATCCTGTTGCTCCTGGTAAATTGATTTTGCAATGTTTGAACTTCTTTTCTTTTTTTTTTTTTTTGTGGTCCTTGAGTTCTGTATTCTAAAGCTTATTTACTACTTTTGGATATCATAGTTAAGGAGATCTTGAAGCTTTGCCCTTCCAAAACCCTGGTAACTCTATATAAGCTTTCAAGCTTCGATACGGTCGATGATTTTCTGCAGAAGGTAGCCGTTATTCGGGGAAAACTTAAGAAGGGGGGAATTGTGGATGTTGGAGCTGCTGCTAGAATTGTTCTGCATGACTGGAACGAAGGTATTTCTGTCTGTTATTTCCAATTGTGTTTTCTTATTAAGAAAAAAACACATTCTAAACTTTCTATTGATATATGAAAAGTGTAAAACAAGTTGAACTTTGTCCCAACTAAACCATTCCTGTTGGACAGAAGAAAATGGCTGCTGCCATACAAAACATAAATAAGTTTTCCAGATGCTGATTTTATTACGGTGCTTGATTGATGTCATAGGTAAAATTCCATATTACACAATGCCACCCGTTAGGAATCAGGTAGAACCTTCAGAGGCAAGGATTGTCACAGAGCTCGGGAAAGAGTTCAACATTGACGAAGTTTATGGCGGTGAATCTTCATTCATTGGGAGCCTCAAATCTGTCAATGATTTCAATCCTGTTGAAGTTCCCCCAAGTTGTCCACTGAATTTTGATGAGAACATGCAAGAGCCAAATGTATGCTTCTTGTTCTTTTATCCTTTTTTACATCTAAATCCCTCTTTAGCTATTTGCATCTTGTGTAAAGATCTGTCTTCTCATAACTGAATGATTTTCTGTTATTTAGAATGGGAATGCTGAGCCAACTATGCAAGACGATGAAGTCCTGGAGAATGCAGAGGATAATAGTGAGGATGAGTCTATGGAGCAGGAAGAAGACGGTGACGATGATGAGAGCAAAGGGAAGGGACAAGATGCAACTAGCAGGCAAAACGAGAAGCTGTATTCAGCAGAAGGTATTCTGAATACTAAAATGAGGAGAGCAGAGAAGAAAAGGAGAAAGAAGGCAAACAATAATAGTAAATCATCAGTGTCTACGGATGCTATGGAAGATGATGACTATGATTTTAAAGTGGATTTCAAAAAGAAGTGATTGATAATCCTAATAGAAAAACCACAACCACAATGAGCTCAGCTGTAAATTTAAAAGTTTTGTGTTATGATTATTATTTAAAATCCAGCTTTTTTCCATAGAATTTTTGTCCTAATGTAAGGAAATTTTGGTTAGTGATATGTTATGTCCTCATTTTATTATTTCGTCTGTAAGGGAAATGGAAACAACTTATTTGTACCGAATTGGAATCATGTTTCAGAATCTTATTTTTGGCTTTGGATGCTCTTTTTATTTTTTCA

mRNA sequence

TCCCGGCTTTCTTTATTTCTCTTAAACCCTATTTCACTTTCAGCTACCCCATCCCTCCCAGCCACTTCACCCCGCCCCGCCGCCGTCTAACCGGCCTTCGCCGGACGCTGCACTCTCAGCTGCCACGGTCTCAGCCTACTGAAGAAGTTGCTTCTGCTCTGTGCCTCTCACAGTCTCTGGCCTCTCCGAGCAAACGGAGATATTAAGTTCCTTATCTCTGTTCCCCCTTAAAAAACACAGTGGTTCAGCCCTTGGCCCGTGCCGTTGGTGAGATCCCGCCGCCGCTCATTTTCAGATCCCCAGTGAAGGCCTCTTGCACAAAGCCCTTGTCTTCTGAGACTCAAAGATGGTGAAGAGGAGCAAAAAGAGTAAAAGCAAGAGGGTTAGTTTAAAGAAGAAGTACAAGATCATTAGAAAGGTGAAGGAGCATCACAAGAAGAAGGCGAAGGAAGCCAAGAAGCTAAGCTTCAAGGGGAAGAGCAAAGTTGAGAAGGACCCGGGTATTCCCAATGATTGGCCCTTCAAGGAACAGGAGCTCAAGGCACTTGAAGCTCGTCGTGCCCGTGCTCTTGATGAGATGGAACAAAAGAAAGCAGCTCGGAAGGAGAGGGCTCAAAAGAGGAAGTTGGGGTTGCTAGATGATGATAATATGGCTTCTGCAGATCAAAGTTTACGAGAGATCAAGGATGGAGATAACTCTGTAGGGCCTGCCCGGAATCGTGATAACTCAGACAGGGTTTTCTACAAGGAATTGGTCAAAGTTATTGAGGCATCAGATGTTATTTTAGAAGTTTTGGATGCACGAGATCCCCTTGGCACCCGTTGTATTGATATGGAGAAGATGGTGATGAAAGCGGGTCCTAATAAGCATCTTGTACTACTTCTGAATAAAATTGATCTTGTCCCTCGAGAGGCTGTTGAGAAGTGGCTTACTTATCTGAGAGAAGAATTGCCTGCTGTTGCCTTCAAGTGTAGCACACAAGAACAAAGGTCCAACTTAGGTTGGAAATCTTCAAAGACATCCAAAACGAAAACAAGCAATTTATTGCAGAGAAGTGACTGCCTTGGAGCTGAAACTCTAATTAAACTGTTGAAGAATTACTCAAGAAGTTATGAGATTAAAAAATCAATAACCGTGGGTGTTATTGGCTTGCCTAATGTTGGCAAAAGCAGTCTTATCAATAGTCTGAAGAGATCCCACGTTGTCAACGTGGGTGCTACACCAGGATTAACAAGATCAATGCAAGAGGTCCATTTAGACAAGAACGTCAAATTGTTGGATTGCCCTGGTGTTGTGATGGTAAGATCTAAAGAAAACGAGCCATCTATAGCTTTGCGGAATTGCAAAAGAATTGAGAAGTTGGAAGATCCTGTTGCTCCTGTTAAGGAGATCTTGAAGCTTTGCCCTTCCAAAACCCTGGTAACTCTATATAAGCTTTCAAGCTTCGATACGGTCGATGATTTTCTGCAGAAGGTAGCCGTTATTCGGGGAAAACTTAAGAAGGGGGGAATTGTGGATGTTGGAGCTGCTGCTAGAATTGTTCTGCATGACTGGAACGAAGGTAAAATTCCATATTACACAATGCCACCCGTTAGGAATCAGGTAGAACCTTCAGAGGCAAGGATTGTCACAGAGCTCGGGAAAGAGTTCAACATTGACGAAGTTTATGGCGGTGAATCTTCATTCATTGGGAGCCTCAAATCTGTCAATGATTTCAATCCTGTTGAAGTTCCCCCAAGTTGTCCACTGAATTTTGATGAGAACATGCAAGAGCCAAATAATGGGAATGCTGAGCCAACTATGCAAGACGATGAAGTCCTGGAGAATGCAGAGGATAATAGTGAGGATGAGTCTATGGAGCAGGAAGAAGACGGTGACGATGATGAGAGCAAAGGGAAGGGACAAGATGCAACTAGCAGGCAAAACGAGAAGCTGTATTCAGCAGAAGGTATTCTGAATACTAAAATGAGGAGAGCAGAGAAGAAAAGGAGAAAGAAGGCAAACAATAATAGTAAATCATCAGTGTCTACGGATGCTATGGAAGATGATGACTATGATTTTAAAGTGGATTTCAAAAAGAAGTGATTGATAATCCTAATAGAAAAACCACAACCACAATGAGCTCAGCTGTAAATTTAAAAGTTTTGTGTTATGATTATTATTTAAAATCCAGCTTTTTTCCATAGAATTTTTGTCCTAATGTAAGGAAATTTTGGTTAGTGATATGTTATGTCCTCATTTTATTATTTCGTCTGTAAGGGAAATGGAAACAACTTATTTGTACCGAATTGGAATCATGTTTCAGAATCTTATTTTTGGCTTTGGATGCTCTTTTTATTTTTTCA

Coding sequence (CDS)

ATGGTGAAGAGGAGCAAAAAGAGTAAAAGCAAGAGGGTTAGTTTAAAGAAGAAGTACAAGATCATTAGAAAGGTGAAGGAGCATCACAAGAAGAAGGCGAAGGAAGCCAAGAAGCTAAGCTTCAAGGGGAAGAGCAAAGTTGAGAAGGACCCGGGTATTCCCAATGATTGGCCCTTCAAGGAACAGGAGCTCAAGGCACTTGAAGCTCGTCGTGCCCGTGCTCTTGATGAGATGGAACAAAAGAAAGCAGCTCGGAAGGAGAGGGCTCAAAAGAGGAAGTTGGGGTTGCTAGATGATGATAATATGGCTTCTGCAGATCAAAGTTTACGAGAGATCAAGGATGGAGATAACTCTGTAGGGCCTGCCCGGAATCGTGATAACTCAGACAGGGTTTTCTACAAGGAATTGGTCAAAGTTATTGAGGCATCAGATGTTATTTTAGAAGTTTTGGATGCACGAGATCCCCTTGGCACCCGTTGTATTGATATGGAGAAGATGGTGATGAAAGCGGGTCCTAATAAGCATCTTGTACTACTTCTGAATAAAATTGATCTTGTCCCTCGAGAGGCTGTTGAGAAGTGGCTTACTTATCTGAGAGAAGAATTGCCTGCTGTTGCCTTCAAGTGTAGCACACAAGAACAAAGGTCCAACTTAGGTTGGAAATCTTCAAAGACATCCAAAACGAAAACAAGCAATTTATTGCAGAGAAGTGACTGCCTTGGAGCTGAAACTCTAATTAAACTGTTGAAGAATTACTCAAGAAGTTATGAGATTAAAAAATCAATAACCGTGGGTGTTATTGGCTTGCCTAATGTTGGCAAAAGCAGTCTTATCAATAGTCTGAAGAGATCCCACGTTGTCAACGTGGGTGCTACACCAGGATTAACAAGATCAATGCAAGAGGTCCATTTAGACAAGAACGTCAAATTGTTGGATTGCCCTGGTGTTGTGATGGTAAGATCTAAAGAAAACGAGCCATCTATAGCTTTGCGGAATTGCAAAAGAATTGAGAAGTTGGAAGATCCTGTTGCTCCTGTTAAGGAGATCTTGAAGCTTTGCCCTTCCAAAACCCTGGTAACTCTATATAAGCTTTCAAGCTTCGATACGGTCGATGATTTTCTGCAGAAGGTAGCCGTTATTCGGGGAAAACTTAAGAAGGGGGGAATTGTGGATGTTGGAGCTGCTGCTAGAATTGTTCTGCATGACTGGAACGAAGGTAAAATTCCATATTACACAATGCCACCCGTTAGGAATCAGGTAGAACCTTCAGAGGCAAGGATTGTCACAGAGCTCGGGAAAGAGTTCAACATTGACGAAGTTTATGGCGGTGAATCTTCATTCATTGGGAGCCTCAAATCTGTCAATGATTTCAATCCTGTTGAAGTTCCCCCAAGTTGTCCACTGAATTTTGATGAGAACATGCAAGAGCCAAATAATGGGAATGCTGAGCCAACTATGCAAGACGATGAAGTCCTGGAGAATGCAGAGGATAATAGTGAGGATGAGTCTATGGAGCAGGAAGAAGACGGTGACGATGATGAGAGCAAAGGGAAGGGACAAGATGCAACTAGCAGGCAAAACGAGAAGCTGTATTCAGCAGAAGGTATTCTGAATACTAAAATGAGGAGAGCAGAGAAGAAAAGGAGAAAGAAGGCAAACAATAATAGTAAATCATCAGTGTCTACGGATGCTATGGAAGATGATGACTATGATTTTAAAGTGGATTTCAAAAAGAAGTGA

Protein sequence

MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFKEQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVGPARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLLNKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCLGAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQEVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVTLYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQVEPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNGNAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNTKMRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKKK
Homology
BLAST of Lsi02G022720 vs. ExPASy Swiss-Prot
Match: Q9M8Z5 (Guanine nucleotide-binding protein-like NSN1 OS=Arabidopsis thaliana OX=3702 GN=NSN1 PE=1 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 4.1e-216
Identity = 415/578 (71.80%), Postives = 481/578 (83.22%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRV+LK+K+K+++KVKEHHKKKAK+AKKL    K +VEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVTLKQKHKVLKKVKEHHKKKAKDAKKLGLHRKPRVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELKALE RRARAL+E+EQKK ARKERA+KRKLGL+DD++  +  +++ ++    N   
Sbjct: 61  EQELKALEVRRARALEEIEQKKEARKERAKKRKLGLVDDEDTKTEGETIEDLPKVVNV-- 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
               RDNS+R FYKELVKVIE SDVILEVLDARDPLGTRC DME+MVM+AGPNKHLVLLL
Sbjct: 121 ----RDNSERAFYKELVKVIELSDVILEVLDARDPLGTRCTDMERMVMQAGPNKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREA EKWL YLREE PAVAFKCSTQEQRSNLGWKSSK S  K SN+LQ SDCL
Sbjct: 181 NKIDLVPREAAEKWLMYLREEFPAVAFKCSTQEQRSNLGWKSSKAS--KPSNMLQTSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GA+TLIKLLKNYSRS+E+KKSITVG+IGLPNVGKSSLINSLKR+HVVNVGATPGLTRS+Q
Sbjct: 241 GADTLIKLLKNYSRSHELKKSITVGIIGLPNVGKSSLINSLKRAHVVNVGATPGLTRSLQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVM++S  N+ SIALRNCKRIEKL+DPV+PVKEILKLCP   LVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMLKSSGNDASIALRNCKRIEKLDDPVSPVKEILKLCPKDMLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYK+ SF+ VDDFL KVA +RGKLKKGG+VD+ AAARIVLHDWNEGKIPYYTMPP R+Q 
Sbjct: 361 LYKIPSFEAVDDFLYKVATVRGKLKKGGLVDIDAAARIVLHDWNEGKIPYYTMPPKRDQG 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNG 480
             +E++IVTEL K+FNIDEVY GESSFIGSLK+VN+FNPV +P + PLNFDE M E    
Sbjct: 421 GHAESKIVTELAKDFNIDEVYSGESSFIGSLKTVNEFNPVIIPSNGPLNFDETMIED--- 480

Query: 481 NAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNTK 540
             E   Q +E  E+  D  +DESM  EE  +++  K K +  T RQN KLY+AE +LNTK
Sbjct: 481 --ESKTQTEEEAEHESD--DDESMGGEE--EEEAGKTKEKSETGRQNVKLYAAESMLNTK 540

Query: 541 MRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKK 579
            ++AEKK+RKKA    K+    + + D DYDFKVD++K
Sbjct: 541 KQKAEKKKRKKA---KKAGADEEDLMDGDYDFKVDYRK 558

BLAST of Lsi02G022720 vs. ExPASy Swiss-Prot
Match: Q54KS4 (Guanine nucleotide-binding protein-like 3 homolog OS=Dictyostelium discoideum OX=44689 GN=gnl3 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 6.6e-97
Identity = 244/605 (40.33%), Postives = 356/605 (58.84%), Query Frame = 0

Query: 6   KKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFKEQELK 65
           K++ SKR SL  K+K+++KV EHH+K  + AK+     KS+  KDPGIPN WPFKE  L 
Sbjct: 3   KQTASKRQSLHHKHKVLKKVAEHHRKVKRFAKQHPELNKSR--KDPGIPNLWPFKEDMLN 62

Query: 66  ALEARRARALDEMEQKKAARK--ERAQKRK---LGLLDDDNMASADQSLREIKDGDNSVG 125
            +E ++ +A +E + KK  R+  + A+ R+       D     S  Q  +++K      G
Sbjct: 63  KIEQQKQKASEEKKNKKEKRRLEQMAEARRDIASMAADAKRRESEFQERQQLKQQQKQQG 122

Query: 126 --PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVL 185
                 +D+S + FY+E+ KVIEA DVIL+VLDARDP+G RC+++EKM+++   NK +VL
Sbjct: 123 KFEKEGKDSSLKQFYREVKKVIEAGDVILQVLDARDPMGCRCLEIEKMILERYTNKKIVL 182

Query: 186 LLNKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSD 245
           +LNKIDLVPRE V  WL YLR   P +AFKCSTQ+Q+ NLG +     +  ++++L  ++
Sbjct: 183 ILNKIDLVPRENVLMWLKYLRNFYPTLAFKCSTQQQKRNLGQQGGIQPELASNDMLNSTE 242

Query: 246 CLGAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRS 305
            LGAE L++LLKNYSRS  IK S+TVG+IG PNVGKSSLINSLKR+  V VGATPG T+ 
Sbjct: 243 SLGAEQLLQLLKNYSRSLNIKTSVTVGIIGYPNVGKSSLINSLKRTRSVGVGATPGFTKF 302

Query: 306 MQEVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTL 365
            QEVHLDKNVKLLD PG+V ++   +E +I LRN  ++EK+ DP+APV  IL  C  K +
Sbjct: 303 AQEVHLDKNVKLLDSPGIVPIKGNVDE-NIILRNVVKLEKVLDPIAPVDAILSRCSQKQI 362

Query: 366 VTLYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRN 425
           + +Y+++ + +  DFL +VA  R K+ KGGI D+ + A  V+ DW  GKIP+YT PP   
Sbjct: 363 LDIYEIAQYQSTTDFLTQVAAKRKKIVKGGIADLRSTAISVIRDWTGGKIPFYTQPP--- 422

Query: 426 QVEPSEARIVTELGKEFNID--EVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQE 485
                ++ ++++   E NID  ++    S+   S  S+    P +      L  D++ ++
Sbjct: 423 -KVLVKSTLLSQFSDELNIDQSDLISTVSNQNTSFASLKYLEPKQQELDNSLFDDDDEED 482

Query: 486 PNNGNAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQ------------DATS 545
                 E    D+E  E  ED  ++E  E EED D+ E     +             AT+
Sbjct: 483 EEEMEDEDEDDDEEEEEEMEDEEDEEDEEDEEDEDEMEEDSDSEALLKYQQQKQVVKATA 542

Query: 546 RQNEKLYSAEGIL-----------NTKMRRAEKKRRKK---ANNNSKSSVSTDAMEDDDY 576
           +Q  ++ S    L           N ++++  KK +KK    + +SK     D  +DDD 
Sbjct: 543 KQVAQIKSKSQNLKDENDQFNPQSNKQLKKQNKKLKKKFGIVSTSSKQVQDDDDEDDDDE 600

BLAST of Lsi02G022720 vs. ExPASy Swiss-Prot
Match: Q21086 (Guanine nucleotide-binding protein-like 3 homolog OS=Caenorhabditis elegans OX=6239 GN=nst-1 PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.8e-85
Identity = 232/584 (39.73%), Postives = 335/584 (57.36%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           M K   K  SKRVS  K+YKI +KV++H++K  KEAKK     K   EK   +PN  PFK
Sbjct: 1   MAKYCLKKTSKRVSCAKRYKIEKKVRDHNRKVKKEAKKNGTTNKK--EKTISVPNSCPFK 60

Query: 61  EQELKALEARRARALDEME-QKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSV 120
           E+ L   E  R +     E  K+AA+  R +KRK  L  +     A  S ++  + D  V
Sbjct: 61  EEILVQAEQEREKIKVRQEAAKEAAKIHRIEKRKNNLPANFESMVAKAS-KQGTEFDKKV 120

Query: 121 GPARNR-------DNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGP 180
             A          D + + +  E+ K +E +DVI++VLDARDPLG+R   +E  V+K G 
Sbjct: 121 ASAAEHEKFNTLDDKTIKAYASEVRKTVEIADVIIQVLDARDPLGSRSKSVEDQVLKGG- 180

Query: 181 NKHLVLLLNKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLG-WKSSKTSKTKTS 240
            K LVLLLNKIDLVPRE V+KWL YLR + P +AFK STQEQ+SN+G + S+  + T+TS
Sbjct: 181 -KRLVLLLNKIDLVPRENVQKWLEYLRGQFPTIAFKASTQEQKSNIGRFNSAILNNTETS 240

Query: 241 NLLQRSDCLGAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGA 300
                  C+GA+ ++K+L NY R+ +IK SI VGV+G PNVGKSS+INSLKR    NVG 
Sbjct: 241 K------CVGADIVMKILANYCRNKDIKTSIRVGVVGFPNVGKSSVINSLKRRKACNVGN 300

Query: 301 TPGLTRSMQEVHLDKNVKLLDCPGVVMVRSKENEP-SIALRNCKRIEKLEDPVAPVKEIL 360
            PG+T+ +QEV LDKN++L+D PGV++V  K+ +P  +AL+N  R++ L DP+APV  IL
Sbjct: 301 LPGITKEIQEVELDKNIRLIDSPGVILVSQKDLDPIEVALKNAIRVDNLLDPIAPVHAIL 360

Query: 361 KLCPSKTLVTLYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPY 420
           + C  +T++  Y L+ F++VD FL ++A   GKL++G   DV AAA+ VL+DWN GK+ Y
Sbjct: 361 RRCSKETIMLHYNLADFNSVDQFLAQLARRIGKLRRGARPDVNAAAKRVLNDWNTGKLRY 420

Query: 421 YTMPPVRNQVEPS---EARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCP 480
           YT PP +   +      A +V++  KEF+ID +   ++  +  L       P+E     P
Sbjct: 421 YTHPPEQGTAKEDIVVPAEVVSQFSKEFDIDAIAEEQNQIVEGL-------PMESDIIAP 480

Query: 481 LNFDENMQEPNNGNAEPTMQDDEVLENAEDNSEDESMEQEEDG--DDDESKGKGQDATSR 540
            N DE   +           DDE+  +  +  +  +  ++  G   DD+     +     
Sbjct: 481 HNSDEEEDD-----------DDEMETDVNEKKQTVTSGRKVKGPTKDDDKPVLPESLALE 540

Query: 541 QNEKLYSAEGILNTKMRRAEKKRRKKANNNSKSSVSTDAMEDDD 570
            N +L     ++ T +++ +KK +K AN   K S S   M   D
Sbjct: 541 GNVQL---NKLIKTAIKKQKKKSKKTANRADKLSDSLGNMLGGD 552

BLAST of Lsi02G022720 vs. ExPASy Swiss-Prot
Match: Q8MT06 (Guanine nucleotide-binding protein-like 3 homolog OS=Drosophila melanogaster OX=7227 GN=Ns1 PE=1 SV=2)

HSP 1 Score: 294.3 bits (752), Expect = 3.1e-78
Identity = 220/591 (37.23%), Postives = 332/591 (56.18%), Query Frame = 0

Query: 2   VKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFKE 61
           +KR K  KSKR++ + K+KI +KV++H+KK+ + AKK   KG SK +K   IPN  PFK+
Sbjct: 3   LKRLKTKKSKRLTGRLKHKIEKKVRDHNKKERRAAKKNPKKG-SKKQKLIQIPNICPFKD 62

Query: 62  QELKAL-EARRARALDEMEQKKAARKERAQ---KRKLGLLDDDNMASADQSLREIKDG-- 121
             LK + EA++ +  + + +++A + ER Q   K    +++D +M S    +    D   
Sbjct: 63  DILKEVEEAKQRQEAERLARREAFKAEREQNKFKTLESMVEDADMRSTVHGIMHENDAQD 122

Query: 122 --DNSVGPARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPN 181
             +     A  ++ S + ++KE  KVIE +DV+LEV+DARDPLGTRC ++E+ V  A  N
Sbjct: 123 QDEKKYKNAVTKEQSLKQYFKEFRKVIENADVVLEVVDARDPLGTRCNEVERAVRGAPGN 182

Query: 182 KHLVLLLNKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNL 241
           K LVL+LNK DLVPRE +  W+ Y R   P  AFK STQ+Q + LG +  K  + KT   
Sbjct: 183 KRLVLVLNKADLVPRENLNNWIKYFRRSGPVTAFKASTQDQANRLGRR--KLREMKTEKA 242

Query: 242 LQRSDCLGAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATP 301
           +Q S C+GAE L+ +L NY R+  IK SI VGV+G+PNVGKSS+INSL R     VG+TP
Sbjct: 243 MQGSVCIGAELLMSMLGNYCRNKGIKTSIRVGVVGIPNVGKSSIINSLTRGRSCMVGSTP 302

Query: 302 GLTRSMQEVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLC 361
           G+T+SMQEV LD  +KL+DCPG+V     EN  ++ L+N +R+  ++DP    + +LK  
Sbjct: 303 GVTKSMQEVELDSKIKLIDCPGIVFTSGGENSHAV-LKNAQRVGDVKDPFTIAESVLKRA 362

Query: 362 PSKTLVTLYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTM 421
             +   T+Y ++++DT ++F  K A   GK  K G+ DV AAAR VL+DWN GKI Y T 
Sbjct: 363 SKEYFCTMYDITNYDTFEEFFAKKAARMGKFLKKGVPDVVAAARSVLNDWNTGKIKYCTQ 422

Query: 422 PP--VRNQVEPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFD 481
           PP     Q     A IV    +EF+++     E+  +    +V   + +E+  + PL   
Sbjct: 423 PPEVQEGQSVHISASIVHSEAREFDVENFESMETEILEHC-AVKTDDIMEITSTGPLEIR 482

Query: 482 ENMQEPNNGN---AEPTMQDDEVLENAEDNSEDESMEQEEDG---DDDESKGKGQDATSR 541
           +  +E    +   A   + + E          DE  E+ +     ++++S  KG     +
Sbjct: 483 QPREEAEPADKITASLVIDEKEKPAKGRKRKLDEEKEKVDPSLLLEENQSLNKG----IK 542

Query: 542 QNEKLYSAEGILNTKMRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDF 577
           Q +KL   + + N      EKK  K  +     S+   + + + YDF  D+
Sbjct: 543 QMQKLKKKQNVRN------EKKISKITDVLDSFSLGPSSSKAEKYDFDEDY 578

BLAST of Lsi02G022720 vs. ExPASy Swiss-Prot
Match: Q9NVN8 (Guanine nucleotide-binding protein-like 3-like protein OS=Homo sapiens OX=9606 GN=GNL3L PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 5.2e-78
Identity = 195/442 (44.12%), Postives = 266/442 (60.18%), Query Frame = 0

Query: 13  VSLKKKYKIIRKVKEHHKKKA----KEAKKLSFKGKSKVEKDPGI--PNDWPFKEQELKA 72
           + L+ K K   +  + HKK +    + AK+   K  SKV   P    PND   +E ELK 
Sbjct: 2   MKLRHKNKKPGEGSKGHKKISWPYPQPAKQNGKKATSKVPSAPHFVHPNDHANREAELK- 61

Query: 73  LEARRARALDEM-EQKKAARKERAQKRKL-------GLLDDDNMASADQSLREIKDGDNS 132
                 + ++EM E+++AAR++  QKR+         L   +     ++ L+E+     +
Sbjct: 62  -----KKWVEEMREKQQAAREQERQKRRTIESYCQDVLRRQEEFEHKEEVLQEL-----N 121

Query: 133 VGPARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVL 192
           + P  + + + + +YKE  KV+E SDVILEVLDARDPLG RC  ME+ V++A  NK LVL
Sbjct: 122 MFPQLDDEATRKAYYKEFRKVVEYSDVILEVLDARDPLGCRCFQMEEAVLRAQGNKKLVL 181

Query: 193 LLNKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSD 252
           +LNKIDLVP+E VEKWL YLR ELP VAFK STQ Q  NL  + S      + +LL+   
Sbjct: 182 VLNKIDLVPKEVVEKWLDYLRNELPTVAFKASTQHQVKNLN-RCSVPVDQASESLLKSKA 241

Query: 253 CLGAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRS 312
           C GAE L+++L NY R  E++  I VGV+GLPNVGKSSLINSLKRS   +VGA PG+T+ 
Sbjct: 242 CFGAENLMRVLGNYCRLGEVRTHIRVGVVGLPNVGKSSLINSLKRSRACSVGAVPGITKF 301

Query: 313 MQEVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTL 372
           MQEV+LDK ++LLD PG+  V    +E    LRNC  ++KL DPV PV+ IL+ C  + +
Sbjct: 302 MQEVYLDKFIRLLDAPGI--VPGPNSEVGTILRNCVHVQKLADPVTPVETILQRCNLEEI 361

Query: 373 VTLYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRN 432
              Y +S F T + FL  VA   GK KKGG+     AA+ VL DW  GKI +Y  PP  +
Sbjct: 362 SNYYGVSGFQTTEHFLTAVAHRLGKKKKGGLYSQEQAAKAVLADWVSGKISFYIPPPATH 421

Query: 433 QVEPS-EARIVTELGKEFNIDE 440
            +     A IV E+ + F+I++
Sbjct: 422 TLPTHLSAEIVKEMTEVFDIED 429

BLAST of Lsi02G022720 vs. ExPASy TrEMBL
Match: A0A0A0KAF7 (CP-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G387750 PE=4 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 7.6e-290
Identity = 540/579 (93.26%), Postives = 556/579 (96.03%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLD DNM S DQS  EIK G++S G
Sbjct: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDADNMDSVDQSSGEIKGGNSSAG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
           PARN DNSDRVF+KEL KVIEASDVILEVLDARDPLGTRC+DMEKMVMKAGPNKHLVLLL
Sbjct: 121 PARNHDNSDRVFFKELAKVIEASDVILEVLDARDPLGTRCMDMEKMVMKAGPNKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL YLREELPAVAFKCSTQEQRSNLGWKSSK SKTKTSNLLQ SDCL
Sbjct: 181 NKIDLVPREAVEKWLNYLREELPAVAFKCSTQEQRSNLGWKSSKASKTKTSNLLQSSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVM+RSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMLRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVD+GAAARIVLHDWNEGKIPYYTMPP+R+QV
Sbjct: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDIGAAARIVLHDWNEGKIPYYTMPPIRSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNG 480
           EPSEARIVTELGK+FNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNN 
Sbjct: 421 EPSEARIVTELGKDFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNE 480

Query: 481 NAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNTK 540
           N EP++QD ++LENA DNSEDESMEQ+E  +DD SK KGQDATSRQNEKLYS EGILNTK
Sbjct: 481 NVEPSIQDGKILENAMDNSEDESMEQDEKDNDDTSKVKGQDATSRQNEKLYSVEGILNTK 540

Query: 541 MRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKKK 580
           MR+AEKKRRKKA NNS SSVSTDAMEDDDYDFKVDFKKK
Sbjct: 541 MRKAEKKRRKKA-NNSISSVSTDAMEDDDYDFKVDFKKK 578

BLAST of Lsi02G022720 vs. ExPASy TrEMBL
Match: A0A6J1GNH5 (LOW QUALITY PROTEIN: guanine nucleotide-binding protein-like NSN1 OS=Cucurbita moschata OX=3662 GN=LOC111456030 PE=4 SV=1)

HSP 1 Score: 997.3 bits (2577), Expect = 2.7e-287
Identity = 538/579 (92.92%), Postives = 554/579 (95.68%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRV+LKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVTLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELK LEARRARALDEMEQKKAARKERAQKRKLGLLDDDNM SAD+SL EI++ D SVG
Sbjct: 61  EQELKTLEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMPSADRSLVEIRNVDKSVG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
            ARNRDNSDR FYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGP+KHLVLLL
Sbjct: 121 SARNRDNSDRXFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPDKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL Y+REELPAVAFKCSTQEQR NLGWKSSKTSKTKTSNLLQRSDCL
Sbjct: 181 NKIDLVPREAVEKWLNYMREELPAVAFKCSTQEQRCNLGWKSSKTSKTKTSNLLQRSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GA+T +KLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GADTXVKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLSSFDTVDDFLQK+AVIRGKLKKGGIVDV AAARIVLHDWNEGKIPYYTMPPVR+QV
Sbjct: 361 LYKLSSFDTVDDFLQKIAVIRGKLKKGGIVDVEAAARIVLHDWNEGKIPYYTMPPVRSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDEN-MQEPNN 480
           EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSV+DF+ VEVPPSCPLN DEN MQEP N
Sbjct: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVDDFSSVEVPPSCPLNLDENIMQEPKN 480

Query: 481 GNAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNT 540
           GN +P+ QDDEVLENA DNS+DESMEQEE  DDDE K  GQDATSRQNEKLYSAEGILNT
Sbjct: 481 GNDQPSTQDDEVLENASDNSDDESMEQEE--DDDEGKTNGQDATSRQNEKLYSAEGILNT 540

Query: 541 KMRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKK 579
           KMRRAEKKRRKKANNN KSS+STDAMED DYDFKVDFKK
Sbjct: 541 KMRRAEKKRRKKANNNCKSSMSTDAMEDXDYDFKVDFKK 577

BLAST of Lsi02G022720 vs. ExPASy TrEMBL
Match: A0A5D3DRD6 (Guanine nucleotide-binding protein-like NSN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G001360 PE=4 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 2.3e-286
Identity = 537/578 (92.91%), Postives = 552/578 (95.50%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLD DNM S DQSL EIKDG++S G
Sbjct: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDTDNMDSVDQSLGEIKDGNSSAG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
           PARN DNSDRVF+KELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL
Sbjct: 121 PARNHDNSDRVFFKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL YLREELPAVAFKCSTQEQRSNLGWKSSK SKTKTSNLLQRSDCL
Sbjct: 181 NKIDLVPREAVEKWLNYLREELPAVAFKCSTQEQRSNLGWKSSKASKTKTSNLLQRSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLD NVKLLDCPGVVM+RSKENEPSIALRNCKRIEKLEDPVA VKEILKLCPSKTLVT
Sbjct: 301 EVHLDNNVKLLDCPGVVMLRSKENEPSIALRNCKRIEKLEDPVASVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLS+FDTVDDFLQKVAVIRGKLKKGGIVD+GAAARIVLHDWNEGKIPYYTMPP+R+QV
Sbjct: 361 LYKLSNFDTVDDFLQKVAVIRGKLKKGGIVDIGAAARIVLHDWNEGKIPYYTMPPIRSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNG 480
           EPSEARIVTELGK+FNI+EVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNN 
Sbjct: 421 EPSEARIVTELGKDFNINEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNE 480

Query: 481 NAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNTK 540
           N E ++QD +VL NA DNSEDESMEQEE  D D SK   +DATS+QNEKLYS EGILNTK
Sbjct: 481 NVERSIQDGKVLGNAMDNSEDESMEQEEKDDGDTSKVMERDATSKQNEKLYSVEGILNTK 540

Query: 541 MRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKK 579
           MRRAEKKRRKKA NNS SSVSTDAMEDDDYDFKVDFKK
Sbjct: 541 MRRAEKKRRKKA-NNSISSVSTDAMEDDDYDFKVDFKK 577

BLAST of Lsi02G022720 vs. ExPASy TrEMBL
Match: A0A6J1JUD1 (LOW QUALITY PROTEIN: guanine nucleotide-binding protein-like NSN1 OS=Cucurbita maxima OX=3661 GN=LOC111488448 PE=4 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 5.1e-286
Identity = 538/579 (92.92%), Postives = 553/579 (95.51%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKR++LKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRITLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQE+KALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNM SADQSL EIKD D SVG
Sbjct: 61  EQEIKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMPSADQSLVEIKDVDKSVG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
            ARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGP+KHLVLLL
Sbjct: 121 SARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPDKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL YLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDC 
Sbjct: 181 NKIDLVPREAVEKWLNYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCF 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GAETLIKLLKNYSRSYEIKKSITVGVI LPNVGKSSLIN LKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GAETLIKLLKNYSRSYEIKKSITVGVIVLPNVGKSSLINGLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLS+FDTVDDFLQK+AVIRGKLK GGIVDV AAARIVLHDWNEGKIPYYTMPPV++QV
Sbjct: 361 LYKLSNFDTVDDFLQKIAVIRGKLKXGGIVDVEAAARIVLHDWNEGKIPYYTMPPVKSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDEN-MQEPNN 480
           EPSEARIV+ELGKEFNIDEVYGGESSFIGSLKSV+DF+ VEVPPSCPLNFDEN MQE  N
Sbjct: 421 EPSEARIVSELGKEFNIDEVYGGESSFIGSLKSVDDFSSVEVPPSCPLNFDENIMQELKN 480

Query: 481 GNAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNT 540
           GN +P+ QDDEVLE A DNS+DESMEQ E  DDDE K KGQDAT  QNEKLYSAEGILNT
Sbjct: 481 GNGQPSTQDDEVLEKASDNSDDESMEQVE--DDDEGKTKGQDATIWQNEKLYSAEGILNT 540

Query: 541 KMRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKK 579
           KMRRAEKKRRKKANNNSKSS+STDAMEDDDYDFKVDFKK
Sbjct: 541 KMRRAEKKRRKKANNNSKSSMSTDAMEDDDYDFKVDFKK 577

BLAST of Lsi02G022720 vs. ExPASy TrEMBL
Match: A0A1S3BSA8 (guanine nucleotide-binding protein-like NSN1 OS=Cucumis melo OX=3656 GN=LOC103492649 PE=4 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.5e-285
Identity = 536/578 (92.73%), Postives = 551/578 (95.33%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLD DNM S DQSL EIKDG++S G
Sbjct: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDADNMDSVDQSLGEIKDGNSSAG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
           PARN DNSDRVF+KELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL
Sbjct: 121 PARNHDNSDRVFFKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL YLREELPAVAFKCSTQEQRSNLGWKSSK SKTKTSNLLQRSDCL
Sbjct: 181 NKIDLVPREAVEKWLNYLREELPAVAFKCSTQEQRSNLGWKSSKASKTKTSNLLQRSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLD NVKLLDCPGVVM+RSKENEPSIALRNCKRIEKLEDPVA VKEILKLCPSKTLVT
Sbjct: 301 EVHLDNNVKLLDCPGVVMLRSKENEPSIALRNCKRIEKLEDPVASVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLS+FDTVDDFLQKVAVIRGKLKKGGIVD+GAAARIVLHDWNEGKIPYYTMPP+R+QV
Sbjct: 361 LYKLSNFDTVDDFLQKVAVIRGKLKKGGIVDIGAAARIVLHDWNEGKIPYYTMPPIRSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNG 480
           EPSEARIVTELGK+FNI+EVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNN 
Sbjct: 421 EPSEARIVTELGKDFNINEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNE 480

Query: 481 NAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNTK 540
           N E ++QD +VL NA DNSEDE MEQEE  D D SK   +DATS+QNEKLYS EGILNTK
Sbjct: 481 NVERSIQDGKVLGNAMDNSEDELMEQEEKDDGDTSKVMERDATSKQNEKLYSVEGILNTK 540

Query: 541 MRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKK 579
           MRRAEKKRRKKA NNS SSVSTDAMEDDDYDFKVDFKK
Sbjct: 541 MRRAEKKRRKKA-NNSISSVSTDAMEDDDYDFKVDFKK 577

BLAST of Lsi02G022720 vs. NCBI nr
Match: XP_038899884.1 (guanine nucleotide-binding protein-like NSN1 [Benincasa hispida])

HSP 1 Score: 1024.2 bits (2647), Expect = 4.3e-295
Identity = 548/580 (94.48%), Postives = 565/580 (97.41%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELKALEARR RALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSL E++ GDNSVG
Sbjct: 61  EQELKALEARRVRALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLGEMEGGDNSVG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
           P  NRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL
Sbjct: 121 PTWNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL YLREELPAVAFKCSTQEQRSNLGWKS+KTSKTKTSN+L+RSDCL
Sbjct: 181 NKIDLVPREAVEKWLNYLREELPAVAFKCSTQEQRSNLGWKSTKTSKTKTSNVLKRSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVM+RSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMLRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVD+GAAARIVLHDWNEGKIPYYTMPP+R+QV
Sbjct: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDIGAAARIVLHDWNEGKIPYYTMPPIRSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNG 480
           EP E+RIVTELGKEFNIDEVYGGE SFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNG
Sbjct: 421 EPLESRIVTELGKEFNIDEVYGGECSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNG 480

Query: 481 NAEPTMQDDEVLENAEDNSEDESMEQEEDGD-DDESKGKGQDATSRQNEKLYSAEGILNT 540
           NAEPTMQDDEV++N  +NSEDES+EQ ED D +D+SKGKGQDATSRQNEKLYSAEGILNT
Sbjct: 481 NAEPTMQDDEVIDN--NNSEDESLEQNEDDDKNDKSKGKGQDATSRQNEKLYSAEGILNT 540

Query: 541 KMRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKKK 580
           KMR+A KK RKKANNNSKSSVSTDAMEDDDYDFKVDFKKK
Sbjct: 541 KMRKAAKKMRKKANNNSKSSVSTDAMEDDDYDFKVDFKKK 578

BLAST of Lsi02G022720 vs. NCBI nr
Match: XP_023547903.1 (guanine nucleotide-binding protein-like NSN1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.8e-293
Identity = 551/579 (95.16%), Postives = 562/579 (97.06%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRV+LKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVTLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNM SADQSL EIKD D SVG
Sbjct: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMPSADQSLVEIKDVDKSVG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
            ARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGP+KHLVLLL
Sbjct: 121 SARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPDKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL YLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL
Sbjct: 181 NKIDLVPREAVEKWLNYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLSSFDTVDDFLQK+AVIRGKLKKGGIVDV AAARIVLHDWNEGKIPYYTMPPVR+QV
Sbjct: 361 LYKLSSFDTVDDFLQKIAVIRGKLKKGGIVDVEAAARIVLHDWNEGKIPYYTMPPVRSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDEN-MQEPNN 480
           EPSEARIV+ELGKEFNIDEVYGGESSFIGSLKSV+DF+ VEVPPSCPLNFDEN MQEP N
Sbjct: 421 EPSEARIVSELGKEFNIDEVYGGESSFIGSLKSVDDFSSVEVPPSCPLNFDENIMQEPKN 480

Query: 481 GNAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNT 540
           GN +P+ QDDEVLENA DNS+DESMEQEE  DDDE K KGQDATSRQNEKLYSAEGILNT
Sbjct: 481 GNDQPSTQDDEVLENASDNSDDESMEQEE--DDDEGKTKGQDATSRQNEKLYSAEGILNT 540

Query: 541 KMRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKK 579
           KMRRAEKKRRKKANNNSKSS+STDAMEDDDYDFKVDFKK
Sbjct: 541 KMRRAEKKRRKKANNNSKSSMSTDAMEDDDYDFKVDFKK 577

BLAST of Lsi02G022720 vs. NCBI nr
Match: KAG7014456.1 (Guanine nucleotide-binding protein-like NSN1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1010.4 bits (2611), Expect = 6.4e-291
Identity = 547/579 (94.47%), Postives = 559/579 (96.55%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRV+LKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVTLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELKALEARRARA DEMEQKKAARKERAQKRKLGLLDDDNM SADQSL EIKD D SVG
Sbjct: 61  EQELKALEARRARAFDEMEQKKAARKERAQKRKLGLLDDDNMPSADQSLVEIKDVDKSVG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
            ARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGP+KHLVLLL
Sbjct: 121 SARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPDKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL Y+REELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL
Sbjct: 181 NKIDLVPREAVEKWLNYMREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLSSFDTVDDFLQK+AVIRGKLKKGGIVDV AAARIVLHDWNEGKI YYTMPPVR+QV
Sbjct: 361 LYKLSSFDTVDDFLQKIAVIRGKLKKGGIVDVEAAARIVLHDWNEGKILYYTMPPVRSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDEN-MQEPNN 480
           EPSEARIV+ELGKEFNIDEVYGGESSFIGSLKSV+DF+ VEVPPSCPLNFDEN MQEP N
Sbjct: 421 EPSEARIVSELGKEFNIDEVYGGESSFIGSLKSVDDFSSVEVPPSCPLNFDENIMQEPKN 480

Query: 481 GNAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNT 540
           GN +P+ QDDEVLENA DNS+DESMEQEE  DDDE K KGQDATSRQNEKLYSAEGILNT
Sbjct: 481 GNDQPSTQDDEVLENASDNSDDESMEQEE--DDDEGKTKGQDATSRQNEKLYSAEGILNT 540

Query: 541 KMRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKK 579
           KMRRAEKKRRKKANNNSK S+STDAMEDDDYDFKVDFKK
Sbjct: 541 KMRRAEKKRRKKANNNSKLSMSTDAMEDDDYDFKVDFKK 577

BLAST of Lsi02G022720 vs. NCBI nr
Match: XP_004146974.1 (guanine nucleotide-binding protein-like NSN1 [Cucumis sativus] >XP_031745139.1 guanine nucleotide-binding protein-like NSN1 [Cucumis sativus] >KGN44791.1 hypothetical protein Csa_016728 [Cucumis sativus])

HSP 1 Score: 1005.7 bits (2599), Expect = 1.6e-289
Identity = 540/579 (93.26%), Postives = 556/579 (96.03%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLD DNM S DQS  EIK G++S G
Sbjct: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDADNMDSVDQSSGEIKGGNSSAG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
           PARN DNSDRVF+KEL KVIEASDVILEVLDARDPLGTRC+DMEKMVMKAGPNKHLVLLL
Sbjct: 121 PARNHDNSDRVFFKELAKVIEASDVILEVLDARDPLGTRCMDMEKMVMKAGPNKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL YLREELPAVAFKCSTQEQRSNLGWKSSK SKTKTSNLLQ SDCL
Sbjct: 181 NKIDLVPREAVEKWLNYLREELPAVAFKCSTQEQRSNLGWKSSKASKTKTSNLLQSSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVM+RSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMLRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVD+GAAARIVLHDWNEGKIPYYTMPP+R+QV
Sbjct: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDIGAAARIVLHDWNEGKIPYYTMPPIRSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNG 480
           EPSEARIVTELGK+FNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNN 
Sbjct: 421 EPSEARIVTELGKDFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNE 480

Query: 481 NAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNTK 540
           N EP++QD ++LENA DNSEDESMEQ+E  +DD SK KGQDATSRQNEKLYS EGILNTK
Sbjct: 481 NVEPSIQDGKILENAMDNSEDESMEQDEKDNDDTSKVKGQDATSRQNEKLYSVEGILNTK 540

Query: 541 MRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKKK 580
           MR+AEKKRRKKA NNS SSVSTDAMEDDDYDFKVDFKKK
Sbjct: 541 MRKAEKKRRKKA-NNSISSVSTDAMEDDDYDFKVDFKKK 578

BLAST of Lsi02G022720 vs. NCBI nr
Match: XP_022953498.1 (LOW QUALITY PROTEIN: guanine nucleotide-binding protein-like NSN1 [Cucurbita moschata])

HSP 1 Score: 997.3 bits (2577), Expect = 5.6e-287
Identity = 538/579 (92.92%), Postives = 554/579 (95.68%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRV+LKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVTLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELK LEARRARALDEMEQKKAARKERAQKRKLGLLDDDNM SAD+SL EI++ D SVG
Sbjct: 61  EQELKTLEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMPSADRSLVEIRNVDKSVG 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
            ARNRDNSDR FYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGP+KHLVLLL
Sbjct: 121 SARNRDNSDRXFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPDKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREAVEKWL Y+REELPAVAFKCSTQEQR NLGWKSSKTSKTKTSNLLQRSDCL
Sbjct: 181 NKIDLVPREAVEKWLNYMREELPAVAFKCSTQEQRCNLGWKSSKTSKTKTSNLLQRSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GA+T +KLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ
Sbjct: 241 GADTXVKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYKLSSFDTVDDFLQK+AVIRGKLKKGGIVDV AAARIVLHDWNEGKIPYYTMPPVR+QV
Sbjct: 361 LYKLSSFDTVDDFLQKIAVIRGKLKKGGIVDVEAAARIVLHDWNEGKIPYYTMPPVRSQV 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDEN-MQEPNN 480
           EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSV+DF+ VEVPPSCPLN DEN MQEP N
Sbjct: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVDDFSSVEVPPSCPLNLDENIMQEPKN 480

Query: 481 GNAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNT 540
           GN +P+ QDDEVLENA DNS+DESMEQEE  DDDE K  GQDATSRQNEKLYSAEGILNT
Sbjct: 481 GNDQPSTQDDEVLENASDNSDDESMEQEE--DDDEGKTNGQDATSRQNEKLYSAEGILNT 540

Query: 541 KMRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKK 579
           KMRRAEKKRRKKANNN KSS+STDAMED DYDFKVDFKK
Sbjct: 541 KMRRAEKKRRKKANNNCKSSMSTDAMEDXDYDFKVDFKK 577

BLAST of Lsi02G022720 vs. TAIR 10
Match: AT3G07050.1 (GTP-binding family protein )

HSP 1 Score: 752.3 bits (1941), Expect = 2.9e-217
Identity = 415/578 (71.80%), Postives = 481/578 (83.22%), Query Frame = 0

Query: 1   MVKRSKKSKSKRVSLKKKYKIIRKVKEHHKKKAKEAKKLSFKGKSKVEKDPGIPNDWPFK 60
           MVKRSKKSKSKRV+LK+K+K+++KVKEHHKKKAK+AKKL    K +VEKDPGIPNDWPFK
Sbjct: 1   MVKRSKKSKSKRVTLKQKHKVLKKVKEHHKKKAKDAKKLGLHRKPRVEKDPGIPNDWPFK 60

Query: 61  EQELKALEARRARALDEMEQKKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVG 120
           EQELKALE RRARAL+E+EQKK ARKERA+KRKLGL+DD++  +  +++ ++    N   
Sbjct: 61  EQELKALEVRRARALEEIEQKKEARKERAKKRKLGLVDDEDTKTEGETIEDLPKVVNV-- 120

Query: 121 PARNRDNSDRVFYKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLL 180
               RDNS+R FYKELVKVIE SDVILEVLDARDPLGTRC DME+MVM+AGPNKHLVLLL
Sbjct: 121 ----RDNSERAFYKELVKVIELSDVILEVLDARDPLGTRCTDMERMVMQAGPNKHLVLLL 180

Query: 181 NKIDLVPREAVEKWLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCL 240
           NKIDLVPREA EKWL YLREE PAVAFKCSTQEQRSNLGWKSSK S  K SN+LQ SDCL
Sbjct: 181 NKIDLVPREAAEKWLMYLREEFPAVAFKCSTQEQRSNLGWKSSKAS--KPSNMLQTSDCL 240

Query: 241 GAETLIKLLKNYSRSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQ 300
           GA+TLIKLLKNYSRS+E+KKSITVG+IGLPNVGKSSLINSLKR+HVVNVGATPGLTRS+Q
Sbjct: 241 GADTLIKLLKNYSRSHELKKSITVGIIGLPNVGKSSLINSLKRAHVVNVGATPGLTRSLQ 300

Query: 301 EVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVT 360
           EVHLDKNVKLLDCPGVVM++S  N+ SIALRNCKRIEKL+DPV+PVKEILKLCP   LVT
Sbjct: 301 EVHLDKNVKLLDCPGVVMLKSSGNDASIALRNCKRIEKLDDPVSPVKEILKLCPKDMLVT 360

Query: 361 LYKLSSFDTVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQV 420
           LYK+ SF+ VDDFL KVA +RGKLKKGG+VD+ AAARIVLHDWNEGKIPYYTMPP R+Q 
Sbjct: 361 LYKIPSFEAVDDFLYKVATVRGKLKKGGLVDIDAAARIVLHDWNEGKIPYYTMPPKRDQG 420

Query: 421 EPSEARIVTELGKEFNIDEVYGGESSFIGSLKSVNDFNPVEVPPSCPLNFDENMQEPNNG 480
             +E++IVTEL K+FNIDEVY GESSFIGSLK+VN+FNPV +P + PLNFDE M E    
Sbjct: 421 GHAESKIVTELAKDFNIDEVYSGESSFIGSLKTVNEFNPVIIPSNGPLNFDETMIED--- 480

Query: 481 NAEPTMQDDEVLENAEDNSEDESMEQEEDGDDDESKGKGQDATSRQNEKLYSAEGILNTK 540
             E   Q +E  E+  D  +DESM  EE  +++  K K +  T RQN KLY+AE +LNTK
Sbjct: 481 --ESKTQTEEEAEHESD--DDESMGGEE--EEEAGKTKEKSETGRQNVKLYAAESMLNTK 540

Query: 541 MRRAEKKRRKKANNNSKSSVSTDAMEDDDYDFKVDFKK 579
            ++AEKK+RKKA    K+    + + D DYDFKVD++K
Sbjct: 541 KQKAEKKKRKKA---KKAGADEEDLMDGDYDFKVDYRK 558

BLAST of Lsi02G022720 vs. TAIR 10
Match: AT1G52980.1 (GTP-binding family protein )

HSP 1 Score: 194.5 bits (493), Expect = 2.3e-49
Identity = 144/452 (31.86%), Postives = 220/452 (48.67%), Query Frame = 0

Query: 81  KKAARKERAQKRKLGLLDDDNMASADQSLREIKDGDNSVGPARNRDNSDRVFYKELVKVI 140
           KKAA  + A + K G          +   R++      V          +  + EL KVI
Sbjct: 160 KKAAESQDAFEEKNGAGPSGEGGEEEDGFRDL------VRHTMFEKGQSKRIWGELYKVI 219

Query: 141 EASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLLNKIDLVPREAVEKWLTYLRE 200
           ++SDVI++V+DARDP GTRC  +EK + +   +KH++LLLNK DLVP  A + WL  L +
Sbjct: 220 DSSDVIVQVIDARDPQGTRCHHLEKTLKEHHKHKHMILLLNKCDLVPAWATKGWLRVLSK 279

Query: 201 ELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCLGAETLIKLLKNYSRSYEIKK 260
           E P +AF  S  +                           G  +L+ +L+ ++R    K+
Sbjct: 280 EYPTLAFHASVNKS-------------------------FGKGSLLSVLRQFARLKSDKQ 339

Query: 261 SITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQEVHLDKNVKLLDCPGVVMVR 320
           +I+VG +G PNVGKSS+IN+L+  +V  V   PG T+  Q + L K + L+DCPGVV  +
Sbjct: 340 AISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPGVV-YQ 399

Query: 321 SKENEPSIALRNCKRIEKLEDPVAPVKEILKLCPSKTLVTLYKLSSFDTVDDFLQKVAVI 380
           S++ E  I L+   R+  LED    + E+L+    + L   YK+  ++   DFL ++   
Sbjct: 400 SRDTETDIVLKGVVRVTNLEDASEHIGEVLRRVKKEHLQRAYKIKDWEDDHDFLLQLCKS 459

Query: 381 RGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTMPPVRNQVEPSEARIVTELGKEFNIDEV 440
            GKL KGG  D+   A+++LHDW  G+IP++  PP  + V      IV  + KE   D  
Sbjct: 460 SGKLLKGGEPDLMTGAKMILHDWQRGRIPFFVPPPKLDNVASESEVIVPGIDKEAIAD-- 519

Query: 441 YGGESSFIGSLKSVNDFNPVEVPPSCPLN---FDENMQEPNNGNAEPTMQDDEVLENAED 500
               S    +LK++      +     P+    +DE   + +    E T  D    EN  D
Sbjct: 520 ---NSQAAAALKAIAGIMSTQQQKDVPVQRDFYDEKDLKDDKKAKESTETD---AENGTD 569

Query: 501 NSEDESMEQEEDGDDDESKGKGQDATSRQNEK 530
             EDE    E+  + D      +DA S  +E+
Sbjct: 580 AEEDEDAVSEDGVESDSD--ADEDAVSENDEE 569

BLAST of Lsi02G022720 vs. TAIR 10
Match: AT2G27200.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 4.9e-31
Identity = 103/363 (28.37%), Postives = 179/363 (49.31%), Query Frame = 0

Query: 133 YKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLLNKIDLVPREAVE 192
           +++L +V+E SD+I+ V+DARDPL  RC D+E    +   +K  +LL+NK DL+P    E
Sbjct: 158 WRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKTMLLVNKADLLPSYVRE 217

Query: 193 KWLTYLREE------LPAVAFKCSTQEQRSNLGWKSSKTSKTKTSN----LLQRSDCLG- 252
           KW  Y            A A   + + +     W++  T++ KT N    +  R D L  
Sbjct: 218 KWAEYFSRNNILFVFWSAKAATATLEGKPLKEQWRAPDTTQ-KTDNPAVKVYGRDDLLDR 277

Query: 253 ----AETLIKLLKNYSRSYEIKKS----ITVGVIGLPNVGKSSLINSLKRSHVVNVGATP 312
               A  ++K+ K+   S    +S    + VG +G PNVGKSS IN+L       V +TP
Sbjct: 278 LKLEALEIVKMRKSRGVSATSTESHCEQVVVGFVGYPNVGKSSTINALVGQKRTGVTSTP 337

Query: 313 GLTRSMQEVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEILKLC 372
           G T+  Q + + +++ L DCPG+V      +   +       I+++ + +  +K + +L 
Sbjct: 338 GKTKHFQTLIISEDLMLCDCPGLVFPSFSSSRYEMVASGVLPIDRMTEHLEAIKVVAELV 397

Query: 373 PSKTLVTLYKLS-----SFD------TVDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHD 432
           P   +  +Y +S     S++         + L+   + RG +   G+ D   AAR +L D
Sbjct: 398 PRHAIEDVYNISLPKPKSYEPQSRPPLASELLRTYCLSRGYVASSGLPDETRAARQILKD 457

Query: 433 WNEGKIPYYTMPPVRNQVEPSEARIVTELG---KEFNIDEVYGGESSFIGSLKSVNDFNP 463
           + EGK+P++ MPP   + + +E    T LG   +E +  E  G E+  +G  + ++D + 
Sbjct: 458 YIEGKLPHFAMPPEITRDDENETADDT-LGAETREGSQTEKKGEEAPSLGLDQVLDDLSS 517

BLAST of Lsi02G022720 vs. TAIR 10
Match: AT1G08410.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 125.6 bits (314), Expect = 1.3e-28
Identity = 89/332 (26.81%), Postives = 157/332 (47.29%), Query Frame = 0

Query: 133 YKELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLLNKIDLVPREAVE 192
           +++L +V+E SD+I+ V+DARDPL  RC D+E    +   +K ++LL+NK DL+P +  E
Sbjct: 158 WRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKIMLLVNKADLLPTDVRE 217

Query: 193 KWLTYLREE------LPAVAFKCSTQEQRSNLGWKS-SKTSKTKTSNLL--QRSDCLG-- 252
           KW  Y R          A+A   + + +     W+      KT   +++   R + L   
Sbjct: 218 KWAEYFRLNNILFVFWSAIAATATLEGKVLKEQWRQPDNLQKTDDPDIMIYGRDELLSRL 277

Query: 253 ---AETLIKLLKNYSRSYEI--------KKSITVGVIGLPNVGKSSLINSLKRSHVVNVG 312
              A+ ++K+  + + S           +    VG +G PNVGKSS IN+L       V 
Sbjct: 278 QFEAQEIVKVRNSRAASVSSQSWTGEYQRDQAVVGFVGYPNVGKSSTINALVGQKRTGVT 337

Query: 313 ATPGLTRSMQEVHLDKNVKLLDCPGVVMVRSKENEPSIALRNCKRIEKLEDPVAPVKEIL 372
           +TPG T+  Q + +   + L DCPG+V      +   +       I+++ +    ++ + 
Sbjct: 338 STPGKTKHFQTLIISDELMLCDCPGLVFPSFSSSRYEMIASGVLPIDRMTEHREAIQVVA 397

Query: 373 KLCPSKTLVTLYKLS-----SFDT------VDDFLQKVAVIRGKLKKGGIVDVGAAARIV 432
              P + + ++Y +S     +++         + L+     RG +   G+ D   AAR++
Sbjct: 398 DKVPRRVIESVYNISLPKPKTYERQSRPPHAAELLKSYCASRGYVASSGLPDETKAARLI 457

BLAST of Lsi02G022720 vs. TAIR 10
Match: AT4G02790.1 (GTP-binding family protein )

HSP 1 Score: 87.4 bits (215), Expect = 4.0e-17
Identity = 85/289 (29.41%), Postives = 128/289 (44.29%), Query Frame = 0

Query: 134 KELVKVIEASDVILEVLDARDPLGTRCIDMEKMVMKAGPNKHLVLLLNKIDLVPREAVEK 193
           KEL + ++  DV++EV DAR PL T    M+  +     N+  +L+LN+ D++  +    
Sbjct: 108 KELREQLKLMDVVIEVRDARIPLSTTHPKMDAWL----GNRKRILVLNREDMISNDDRND 167

Query: 194 WLTYLREELPAVAFKCSTQEQRSNLGWKSSKTSKTKTSNLLQRSDCLGAETLIKLLKNYS 253
           W  Y  ++   V F          LG  + K  +   S      D  G      LL    
Sbjct: 168 WARYFAKQGIKVIF------TNGKLGMGAMKLGRLAKS---LAGDVNGKRREKGLL---- 227

Query: 254 RSYEIKKSITVGVIGLPNVGKSSLINSLKRSHVVNVGATPGLTRSMQEVHLDKNVKLLDC 313
                 +S+  G+IG PNVGKSSLIN L +  +      PG+TR M+ V L K++ LLD 
Sbjct: 228 -----PRSVRAGIIGYPNVGKSSLINRLLKRKICAAAPRPGVTREMKWVKLGKDLDLLDS 287

Query: 314 PGVVMVRSKENEPSIALRNCKRI-EK---LEDPVAPVKEILKLCPSKTLVTLYKLSSFDT 373
           PG++ +R  +   +I L  C  I EK     D    + ++L   P      LY       
Sbjct: 288 PGMLPMRIDDQAAAIKLAICDDIGEKAYDFTDVAGILVQMLARIPEVGAKALYNRYKIQL 347

Query: 374 VDDFLQKVAVIRGKLKKGGIVDVGAAARIVLHDWNEGKIPYYTM--PPV 417
             +  +K     G    GG  D   AA  +L D+ +GK  Y ++  PP+
Sbjct: 348 EGNCGKKFVKTLGLNLFGG--DSHQAAFRILTDFRKGKFGYVSLERPPL 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8Z54.1e-21671.80Guanine nucleotide-binding protein-like NSN1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q54KS46.6e-9740.33Guanine nucleotide-binding protein-like 3 homolog OS=Dictyostelium discoideum OX... [more]
Q210865.8e-8539.73Guanine nucleotide-binding protein-like 3 homolog OS=Caenorhabditis elegans OX=6... [more]
Q8MT063.1e-7837.23Guanine nucleotide-binding protein-like 3 homolog OS=Drosophila melanogaster OX=... [more]
Q9NVN85.2e-7844.12Guanine nucleotide-binding protein-like 3-like protein OS=Homo sapiens OX=9606 G... [more]
Match NameE-valueIdentityDescription
A0A0A0KAF77.6e-29093.26CP-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G387750 P... [more]
A0A6J1GNH52.7e-28792.92LOW QUALITY PROTEIN: guanine nucleotide-binding protein-like NSN1 OS=Cucurbita m... [more]
A0A5D3DRD62.3e-28692.91Guanine nucleotide-binding protein-like NSN1 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1JUD15.1e-28692.92LOW QUALITY PROTEIN: guanine nucleotide-binding protein-like NSN1 OS=Cucurbita m... [more]
A0A1S3BSA81.5e-28592.73guanine nucleotide-binding protein-like NSN1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
Match NameE-valueIdentityDescription
XP_038899884.14.3e-29594.48guanine nucleotide-binding protein-like NSN1 [Benincasa hispida][more]
XP_023547903.11.8e-29395.16guanine nucleotide-binding protein-like NSN1 [Cucurbita pepo subsp. pepo][more]
KAG7014456.16.4e-29194.47Guanine nucleotide-binding protein-like NSN1 [Cucurbita argyrosperma subsp. argy... [more]
XP_004146974.11.6e-28993.26guanine nucleotide-binding protein-like NSN1 [Cucumis sativus] >XP_031745139.1 g... [more]
XP_022953498.15.6e-28792.92LOW QUALITY PROTEIN: guanine nucleotide-binding protein-like NSN1 [Cucurbita mos... [more]
Match NameE-valueIdentityDescription
AT3G07050.12.9e-21771.80GTP-binding family protein [more]
AT1G52980.12.3e-4931.86GTP-binding family protein [more]
AT2G27200.14.9e-3128.37P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G08410.11.3e-2826.81P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G02790.14.0e-1729.41GTP-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 64..84
NoneNo IPR availablePIRSRPIRSR006230-1PIRSR006230-1coord: 134..415
e-value: 2.6E-43
score: 146.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..100
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 463..579
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 562..579
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 471..485
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 489..512
NoneNo IPR availablePANTHERPTHR11089GTP-BINDING PROTEIN-RELATEDcoord: 1..577
NoneNo IPR availablePANTHERPTHR11089:SF37GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE NSN1coord: 1..577
NoneNo IPR availableCDDcd04178Nucleostemin_likecoord: 144..316
e-value: 9.52364E-106
score: 313.358
IPR006073GTP binding domainPRINTSPR00326GTP1OBGcoord: 310..325
score: 26.29
coord: 264..284
score: 48.93
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 264..324
e-value: 8.8E-15
score: 54.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 133..319
e-value: 1.4E-47
score: 163.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 132..349
IPR023179GTP-binding protein, orthogonal bundle domain superfamilyGENE3D1.10.1580.10coord: 321..415
e-value: 3.6E-10
score: 41.8
IPR014813Guanine nucleotide-binding protein-like 3, N-terminal domainPFAMPF08701GN3L_Grn1coord: 16..91
e-value: 1.0E-18
score: 67.2
IPR030378Circularly permuted (CP)-type guanine nucleotide-binding (G) domainPROSITEPS51721G_CPcoord: 133..319
score: 42.92453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G022720.1Lsi02G022720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005730 nucleolus
molecular_function GO:0005525 GTP binding