Lsi02G022710 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G022710
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionsucrose-binding protein-like
Locationchr02: 29302408 .. 29306858 (-)
RNA-Seq ExpressionLsi02G022710
SyntenyLsi02G022710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAATTTCTCATGAATCCAACTTGGAAATTGAATCACAGAGCTTTAGCTTCATCATAAAAAAAAACCGGCTATCTCCATCAAAATCTCATTACGAAGCTTCAAGTTCCTCCATTAATGGACACGTAAACAGGACACCAGTCCTCACGCATGAGGCCACAAATCCCTTCATATAATCCCTAAACCCTTCTTCCATTTTCCTGCACTTCTCTGCAATAACAATGGCTTCCAAATTCAACCTCTCTCTCCTCCTCCTCCTTTTCCTCCTCTTTGTCGCCGCCGCCTGCCTCGCTTCCAAAGACCCTGAGTTGAAGCAGTGCAAGCACCAGTGCAAAGTACAGCGCCAGTTCGATGAGAAGCAGAGGCGGGATTGCGAGAGAAGTTGCGATGAGTATTATAAGATGAAGAAGGAAAGAGAAAGAGAAAAAGGAAGGAATTACGAAAGCGAAGAAGAAGAAGAAGAGGAAGAGGAGGAGAAGAATCCGTACGTGTTTGAGGATGAGCATTTTGAAGGTAAGTTTGAAACAGGGGAAGGCAGAATTAGGGTTCTTCAGAAATTCACTCAGCGATCGGAACTTCTTCGAGGGATTGAGAATTTTAGGGTTTCGATCGTGGAGGCTAATCCTTTCACTTTCGTCATTCCTACCCATTTTGATGCTGAAATCATTCTCTTTGTTGCTCGGGGTATGCCATTTTCTTATTTCCTTTTCTTCTAACTTTTATTTTAGACATTTAAATTAAAAAATATATAACTTTTTTTTTTTTTTTTAGTTTCCTCGACTCAAATAACATTTATTAAATACAGGTTGGATTCGATTAGTTCAAATTATTTGGATAATTTATTAAAAAAATTTATTTAAAAATAATTAAATGTTAATATATAAACTTTTTGTTAAACTGATTAATCTCTCCACTTTTCATTCCAAAGTCCTAAAAAATTATATTTGAAGTGGTGGAAGAATATAAATTATCTAAAATAACCATGGTATTAAATAAAAATAAATTAAATATACCTATACCTAATTGTATCATAATTTAAAAAACATTTTTAAAATTAATACTAGGGTGGGGTTGGTTGGAGTGTTCGGGTTATTTGGGGCAATCCATGAACCAACCCTAACCTATAGATTTTCCATTTATTTGGACTTATCTGAAGTGTACTATATGATTTTTTAAATTTAGTTTATTTAATTTAGAATTTAAAATATTATTGTTCATCTAATACTAAGCCAGTTTTAGTCTATTCAATAAATTTTAAATTTAATTTTAAGAGCTACTTGTTTAAATTATTTTGATAAAAATGATTTAAAATACACTCCACAAACTATATTCCAAGCACAAAATAGTTTTTCAAGAGATTTTAAATTGCATTTCAAACCGTTTTTATTGAAAAAGTTTAAATACAAATGAGTTTTTTTGAAAAATCATGTCAATGCTTTTATAAGTCAACCATAAGCATGAACTAAATTTAAGAACTATTGTAAGTATAAGATTAAGTTAGGATATTTAAAAATATAGTCTAAAATGGAACCATATCCCAACAACAGGATCCACCATGATACTTTAACCAAAATTAAATCAAACTTATAATAAGAGCTATTTTAGATAATTTTAAGATTTATTAAGAGTATCAACATGTGTCCGTGGTTCAATTTCTTCCACCCTTATTTGTATTTGTTCTAAAAAAATATATATTAATTAAGAGTACATGACTGGATCGTAGAGATTGGAGAGAAACTAAAATAAGATAATTATTTAAGTTAGAATCAAAATGGTCAACCAATTTTTTTTCTAAAAAAAAATCAAAATACGAGTGATTAAATTGAAAGACAATCATACTTGAGATTTTTTATTTTTTGGGTTATGGATTATGGTTGGGGTTAAGGTGCAAGGATTGAAATTTTGGTCGATATATCTGTAAACTTTGAGTCTTAAAATCGATATTAAATATTAATAGTGGTCTCTAATATCTTTTAGGAATACACATAAAACATAACAATTTACCATCTTTTTATCTCAATACGAATATGCATATTAATGAAAGCTTTTTAACTCAAATTTTTACTAATAGAAAAAATCTCAAACTATTTATAGTAAACATAAAAAAAAAAAAAAAAGATAGAGTTCTATTAACATCCATGATAGACTTCTATCATTTCTATTATTGATAGACTTCTATCGACATCTATCATAACTATCTAAATTTTTTTTTATTTTCTATAGTTTATCTTATTTTTCTATTTTTAAAAATTTCTTTAAATACAATGTAATTATTAATTTACATACTTTGAATATTTCTGTACAATTAAAATATAATATTTTAATCCTTAGTAAGGTGTTTGTGAAAATGACATTGGGGAATTTTGTGTTGGTTTACGAAGGACGAGGTACGATAACAGTGATTAAGGAAAAGAGAGCAAGCTTTGATGTGAAATGTGGAGATGTTTTCAGAGTTCCATCTGGAGCTCCCTTTTATTTCATAAACAAGGACGAACATCAAAAGCTCAAGATTGTTAAGCTTCTCCAACCCACATCTGTTCCTGGACATTTTGAGGTAAAACCCTCAAATTTCTCCCTGTTTTTCTGTCAAATGATATAATATTACACATTTCATAGTCAAAATCTGTTAGGATGTTGGATGAAATTGATACTTGAATGAGTGTGGTAGCGATATGGAACTGTGTAGGCTCTGTCTCTAAGTCTTAATTTCCGTTCTAATGACCGTCATAGTTCCAATTTTCGTATAGGGATAGTTTTGAAACCATTATGATATAAGGTCTAGGGCAATAGATTTAGTTCAGGATGTTTTTTCAATTTGGTCTTTTTGTTTTGTCGAATTCGTCTGAGAAATGAATGTTTCAAGCAGATTTTCCAGCCAGCTGGTGGTGAAAACTCAGAATCATTCTACACAGCATTCAGCTGGGATTTGCTTGAAGCAGCATTCAAGGTTATAAAAAAGACCTTCCCGAACTGCCTAAACAGTGTGTTTGGTTTATATATATATATATATATATATATATATATGCTTATTTTTTCTTGTATTCCTACAAAACTTTCTTGGCTGCCAAACAGTTTCCAAGAGATAAGTTGGAGAGATTTTTCAAGCAACAAAAAGCAGGGACCATAATCAAGGCTTCTAGAGAACAGATCCGATCACTTAGCCAACATGAAGAAATCATCCCAAAGATCTGGCCATTCTCAGAAGGTGAAACTGAGCGTCCATTCAATCTGCTCAAGCAACATCCATGGCAGTCAAATAAGTTCGGTCGTCTCTTTGAAGCCTATCCTGATGAGTTCAGCCAGCTTCGGGACCTTGGTGTCGCCATTGCCTTTGCCAACCTGACCAAAGTATAATTTACATCTACTTCATAGGGTGGCTATGTATGAAACTCATTTGTCAATTTCAGGTTGTTTATTTTACTTTTGCTGTGTGCTTCTTTTTTGAAGGGTTCAATGATGGCTCCTCACTATAACTCCAGAGCCATGAAGATAGCTGTGGTGGTGGATGGCGAAGGGGGGTTCCAAATGGCGTGTCCCCACCTCTCATCATCTTCTGGAAGAAGTGGTCGATGGTCGGAGCCAGAGCAAGAGCGGAAAGGCGAGAGGACTTACCAGAAGATTAGAGGGCGGCTGAGACGTGGGGTGGTGTTCATAGTTCCGGCAGGTCATCCTTTTTCAGTCTTTGCCTCACCTAACCACAACCTCGAGATTGTTTGCTTTGAAGTGAACGCCTATGGCAACACCAAGTACCTTCTAGCAGGTATAAAATCAATCTGATCAACAGGCTTTAACCATATTCAAAACTTAGATGTTTACACTGTAATAGGCTAGACGGGCGGGTCTTGCAGTCAAGTTTCTTTTCCCTCTTCGCACGAGGAAATTTTTGCTACCTTATTTGAATCCAAAATCATAGACAGCAATTGTTTTATTTTTCTTCAAACATAACATTTTGGATATTTGAGCTCTCGACCCAACTCAATATCTCATGCTTTCTTTGGCACTAAGTACTTAAATTCTTTCGAAGTTTTTGGTAAGCTGACTTCTGACCAAGAACATTCATTGCTAAAATATTGAGCTATCTGTTTACAGGGAAAGAGAACATAGTGAACAAGATGGAGAGGGTAGCAAGAGAGTTGGGATTCAACACACCAGGAAGAGAAGTTGAAAGAATGTTCAGGCAGCAAGAAGAAGAGTTTTTCTTCCCAGGACCAAACCAACAAGAACAAGAATGGGCTGAAGCCTGAACTGAAGGTGGGTACTTAAAGAAAACCCACCAAAGCCCTAGAGGGACATAGAAATGTAAATATGTGAAAATATCTTAGGCTCTAGCTACTCCCCTCTTGTACAGGCTTTTGCATAAATAAAGTGAGTTTCTTTCTACTATATTATATGGGTTTTAAGTATCATCATTTTGTTTTCAAATCGTTTATCTAATAAAATATTAATTTGTCA

mRNA sequence

TATAATTTCTCATGAATCCAACTTGGAAATTGAATCACAGAGCTTTAGCTTCATCATAAAAAAAAACCGGCTATCTCCATCAAAATCTCATTACGAAGCTTCAAGTTCCTCCATTAATGGACACGTAAACAGGACACCAGTCCTCACGCATGAGGCCACAAATCCCTTCATATAATCCCTAAACCCTTCTTCCATTTTCCTGCACTTCTCTGCAATAACAATGGCTTCCAAATTCAACCTCTCTCTCCTCCTCCTCCTTTTCCTCCTCTTTGTCGCCGCCGCCTGCCTCGCTTCCAAAGACCCTGAGTTGAAGCAGTGCAAGCACCAGTGCAAAGTACAGCGCCAGTTCGATGAGAAGCAGAGGCGGGATTGCGAGAGAAGTTGCGATGAGTATTATAAGATGAAGAAGGAAAGAGAAAGAGAAAAAGGAAGGAATTACGAAAGCGAAGAAGAAGAAGAAGAGGAAGAGGAGGAGAAGAATCCGTACGTGTTTGAGGATGAGCATTTTGAAGGTAAGTTTGAAACAGGGGAAGGCAGAATTAGGGTTCTTCAGAAATTCACTCAGCGATCGGAACTTCTTCGAGGGATTGAGAATTTTAGGGTTTCGATCGTGGAGGCTAATCCTTTCACTTTCGTCATTCCTACCCATTTTGATGCTGAAATCATTCTCTTTGTTGCTCGGGGACGAGGTACGATAACAGTGATTAAGGAAAAGAGAGCAAGCTTTGATGTGAAATGTGGAGATGTTTTCAGAGTTCCATCTGGAGCTCCCTTTTATTTCATAAACAAGGACGAACATCAAAAGCTCAAGATTGTTAAGCTTCTCCAACCCACATCTGTTCCTGGACATTTTGAGATTTTCCAGCCAGCTGGTGGTGAAAACTCAGAATCATTCTACACAGCATTCAGCTGGGATTTGCTTGAAGCAGCATTCAAGTTTCCAAGAGATAAGTTGGAGAGATTTTTCAAGCAACAAAAAGCAGGGACCATAATCAAGGCTTCTAGAGAACAGATCCGATCACTTAGCCAACATGAAGAAATCATCCCAAAGATCTGGCCATTCTCAGAAGGTGAAACTGAGCGTCCATTCAATCTGCTCAAGCAACATCCATGGCAGTCAAATAAGTTCGGTCGTCTCTTTGAAGCCTATCCTGATGAGTTCAGCCAGCTTCGGGACCTTGGTGTCGCCATTGCCTTTGCCAACCTGACCAAAGGTTCAATGATGGCTCCTCACTATAACTCCAGAGCCATGAAGATAGCTGTGGTGGTGGATGGCGAAGGGGGGTTCCAAATGGCGTGTCCCCACCTCTCATCATCTTCTGGAAGAAGTGGTCGATGGTCGGAGCCAGAGCAAGAGCGGAAAGGCGAGAGGACTTACCAGAAGATTAGAGGGCGGCTGAGACGTGGGGTGGTGTTCATAGTTCCGGCAGGTCATCCTTTTTCAGTCTTTGCCTCACCTAACCACAACCTCGAGATTGTTTGCTTTGAAGTGAACGCCTATGGCAACACCAAGTACCTTCTAGCAGGGAAAGAGAACATAGTGAACAAGATGGAGAGGGTAGCAAGAGAGTTGGGATTCAACACACCAGGAAGAGAAGTTGAAAGAATGTTCAGGCAGCAAGAAGAAGAGTTTTTCTTCCCAGGACCAAACCAACAAGAACAAGAATGGGCTGAAGCCTGAACTGAAGGTGGGTACTTAAAGAAAACCCACCAAAGCCCTAGAGGGACATAGAAATGTAAATATGTGAAAATATCTTAGGCTCTAGCTACTCCCCTCTTGTACAGGCTTTTGCATAAATAAAGTGAGTTTCTTTCTACTATATTATATGGGTTTTAAGTATCATCATTTTGTTTTCAAATCGTTTATCTAATAAAATATTAATTTGTCA

Coding sequence (CDS)

ATGGCTTCCAAATTCAACCTCTCTCTCCTCCTCCTCCTTTTCCTCCTCTTTGTCGCCGCCGCCTGCCTCGCTTCCAAAGACCCTGAGTTGAAGCAGTGCAAGCACCAGTGCAAAGTACAGCGCCAGTTCGATGAGAAGCAGAGGCGGGATTGCGAGAGAAGTTGCGATGAGTATTATAAGATGAAGAAGGAAAGAGAAAGAGAAAAAGGAAGGAATTACGAAAGCGAAGAAGAAGAAGAAGAGGAAGAGGAGGAGAAGAATCCGTACGTGTTTGAGGATGAGCATTTTGAAGGTAAGTTTGAAACAGGGGAAGGCAGAATTAGGGTTCTTCAGAAATTCACTCAGCGATCGGAACTTCTTCGAGGGATTGAGAATTTTAGGGTTTCGATCGTGGAGGCTAATCCTTTCACTTTCGTCATTCCTACCCATTTTGATGCTGAAATCATTCTCTTTGTTGCTCGGGGACGAGGTACGATAACAGTGATTAAGGAAAAGAGAGCAAGCTTTGATGTGAAATGTGGAGATGTTTTCAGAGTTCCATCTGGAGCTCCCTTTTATTTCATAAACAAGGACGAACATCAAAAGCTCAAGATTGTTAAGCTTCTCCAACCCACATCTGTTCCTGGACATTTTGAGATTTTCCAGCCAGCTGGTGGTGAAAACTCAGAATCATTCTACACAGCATTCAGCTGGGATTTGCTTGAAGCAGCATTCAAGTTTCCAAGAGATAAGTTGGAGAGATTTTTCAAGCAACAAAAAGCAGGGACCATAATCAAGGCTTCTAGAGAACAGATCCGATCACTTAGCCAACATGAAGAAATCATCCCAAAGATCTGGCCATTCTCAGAAGGTGAAACTGAGCGTCCATTCAATCTGCTCAAGCAACATCCATGGCAGTCAAATAAGTTCGGTCGTCTCTTTGAAGCCTATCCTGATGAGTTCAGCCAGCTTCGGGACCTTGGTGTCGCCATTGCCTTTGCCAACCTGACCAAAGGTTCAATGATGGCTCCTCACTATAACTCCAGAGCCATGAAGATAGCTGTGGTGGTGGATGGCGAAGGGGGGTTCCAAATGGCGTGTCCCCACCTCTCATCATCTTCTGGAAGAAGTGGTCGATGGTCGGAGCCAGAGCAAGAGCGGAAAGGCGAGAGGACTTACCAGAAGATTAGAGGGCGGCTGAGACGTGGGGTGGTGTTCATAGTTCCGGCAGGTCATCCTTTTTCAGTCTTTGCCTCACCTAACCACAACCTCGAGATTGTTTGCTTTGAAGTGAACGCCTATGGCAACACCAAGTACCTTCTAGCAGGGAAAGAGAACATAGTGAACAAGATGGAGAGGGTAGCAAGAGAGTTGGGATTCAACACACCAGGAAGAGAAGTTGAAAGAATGTTCAGGCAGCAAGAAGAAGAGTTTTTCTTCCCAGGACCAAACCAACAAGAACAAGAATGGGCTGAAGCCTGA

Protein sequence

MASKFNLSLLLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYKMKKEREREKGRNYESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELLRGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVFRVPSGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKFPRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQSNKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMACPHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQEQEWAEA
Homology
BLAST of Lsi02G022710 vs. ExPASy Swiss-Prot
Match: A0A2I4E5L6 (Vicilin Jug r 6.0101 OS=Juglans regia OX=51240 GN=LOC108986502 PE=1 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.1e-157
Identity = 291/496 (58.67%), Postives = 376/496 (75.81%), Query Frame = 0

Query: 4   KFNLSLLLLLFLLFVAAA-CLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYKMK 63
           K  ++LLLL  LL + A   LA +DPELKQCKHQC+ QRQFDE+++  C+RSCDEY+  K
Sbjct: 6   KIPIALLLLTSLLAICAGLALAMQDPELKQCKHQCRHQRQFDEQEKEHCQRSCDEYHIEK 65

Query: 64  KERER------EKGRNYESEEEEEE---EEEEKNPYVFEDEHFEGKFETGEGRIRVLQKF 123
           K RER      E+G + E   EEEE   E EE+NPYVFEDE FE +  T EGRI+VL+KF
Sbjct: 66  KARERAERRRSEEGSSREEGYEEEELGGEREEENPYVFEDEDFETRVRTDEGRIQVLEKF 125

Query: 124 TQRSELLRGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIK-EKRASFDVK 183
           T+RS+LLRGIENFRV+I+EANP TF+ P HFDAE+++FVA+GR TIT ++ EKR +F+V+
Sbjct: 126 TKRSKLLRGIENFRVAILEANPQTFISPAHFDAELVVFVAKGRATITTVREEKRENFNVE 185

Query: 184 CGDVFRVPSGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWD 243
            GD+ R+P+G P Y IN+DE++KL IVK+L+P SVPGHFE F  +GGE+ ESFY AFSW+
Sbjct: 186 QGDIMRIPAGTPVYLINRDENEKLYIVKILRPVSVPGHFEAFHGSGGEDPESFYRAFSWE 245

Query: 244 LLEAAFKFPRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNL 303
           +LEAA K  RD+LE+ F +Q  G IIKAS+EQIRS+S+HEE  P+IWPF  G++  PFNL
Sbjct: 246 VLEAALKTRRDQLEKLFGKQTQGVIIKASKEQIRSMSKHEETTPRIWPFG-GDSTHPFNL 305

Query: 304 LKQHPWQSNKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDG 363
             + P QSN+FGRLFE  P E  QL+DL + ++FAN+TKGSM  P+YNSRA KI+VV++G
Sbjct: 306 FHKRPSQSNQFGRLFETDPKECKQLQDLDLMVSFANITKGSMAGPYYNSRATKISVVIEG 365

Query: 364 EGGFQMACPHLSSSSGRSGRWSEPEQERKGER--TYQKIRGRLRRGVVFIVPAGHPFSVF 423
           EG F+MACPHLSSS  R  R       R+     +YQ+IRGRLR G+VF+ PAGHP +V 
Sbjct: 366 EGYFEMACPHLSSSGSRGQREGSGSSRRRSRSGPSYQQIRGRLRPGMVFVAPAGHPVAVI 425

Query: 424 ASPNHNLEIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEE 483
           AS N NL+++CF+VNA GN ++ LAGK NIVN+ E+ A+EL FN P REVE++FR Q++E
Sbjct: 426 ASRNKNLQVLCFDVNAQGNIRFPLAGKNNIVNEFEKEAKELAFNFPAREVEKIFRNQDQE 485

Query: 484 FFFPGPNQQEQEWAEA 487
           FFFPGP++Q +E   A
Sbjct: 486 FFFPGPSRQPEEGGRA 500

BLAST of Lsi02G022710 vs. ExPASy Swiss-Prot
Match: Q8S4P9 (Vicilin Cor a 11.0101 OS=Corylus avellana OX=13451 PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 4.9e-154
Identity = 274/461 (59.44%), Postives = 363/461 (78.74%), Query Frame = 0

Query: 23  LASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYKMKKEREREKGRNYESEEEEEEE 82
           L  +DPELK+CKH+C+ +RQFDE+QRRD ++ C+E     +ER++E+G    S EE   +
Sbjct: 2   LPKEDPELKKCKHKCRDERQFDEQQRRDGKQICEE---KARERQQEEG---NSSEESYGK 61

Query: 83  EEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELLRGIENFRVSIVEANPFTFVIPT 142
           E+E+NPYVF+DEHFE + +T EGR++VL+ FT+RS LL GIENFR++I+EANP TF+ P 
Sbjct: 62  EQEENPYVFQDEHFESRVKTEEGRVQVLENFTKRSRLLSGIENFRLAILEANPHTFISPA 121

Query: 143 HFDAEIILFVARGRGTITVIK-EKRASFDVKCGDVFRVPSGAPFYFINKDEHQKLKIVKL 202
           HFDAE++LFVA+GR TIT+++ EKR SF+V+ GD+ R+P+G P Y IN+DE++KL IVK+
Sbjct: 122 HFDAELVLFVAKGRATITMVREEKRESFNVEHGDIIRIPAGTPVYMINRDENEKLFIVKI 181

Query: 203 LQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKFPRDKLERFFKQQKAGTIIKAS 262
           LQP S PGHFE F  AGGE+ ESFY AFSW++LEAA K  R++LE+ F +Q  G+I+KAS
Sbjct: 182 LQPVSAPGHFEAFYGAGGEDPESFYRAFSWEVLEAALKVRREQLEKVFGEQSKGSIVKAS 241

Query: 263 REQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQSNKFGRLFEAYPDEFSQLRDLG 322
           RE+IR+LSQHEE  P+IWPF  GE+  P NLL +HP QSN+FGRL+EA+PD+  QL+DL 
Sbjct: 242 REKIRALSQHEEGPPRIWPFG-GESSGPINLLHKHPSQSNQFGRLYEAHPDDHKQLQDLD 301

Query: 323 VAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMACPHLSSSSGRSGRWSEPEQERK 382
           + ++FAN+TKGSM  P+YNSRA KI+VVV+GEG F+MACPHLSSSSG             
Sbjct: 302 LMVSFANITKGSMAGPYYNSRATKISVVVEGEGFFEMACPHLSSSSG------------- 361

Query: 383 GERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIVCFEVNAYGNTKYLLAGKENIV 442
              +YQKI  RLRRGVVF+ PAGHP +V AS N+NL+++CFEVNA+GN+++ LAGK NIV
Sbjct: 362 ---SYQKISARLRRGVVFVAPAGHPVAVIASQNNNLQVLCFEVNAHGNSRFPLAGKGNIV 421

Query: 443 NKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQEQE 483
           N+ ER A+EL FN P REVER+F+ Q++ FFFPGPN+Q++E
Sbjct: 422 NEFERDAKELAFNLPSREVERIFKNQDQAFFFPGPNKQQEE 439

BLAST of Lsi02G022710 vs. ExPASy Swiss-Prot
Match: B4X640 (Vicilin Pis v 3.0101 OS=Pistacia vera OX=55513 PE=1 SV=2)

HSP 1 Score: 469.9 bits (1208), Expect = 3.4e-131
Identity = 263/549 (47.91%), Postives = 355/549 (64.66%), Query Frame = 0

Query: 3   SKFNLSLLLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYKMK 62
           +KF L+L L+  L+  A   LA  DPELKQCKHQCKVQRQ+DE+Q+  C + C++YYK K
Sbjct: 5   TKFCLTLFLVSVLILCAGLALAKTDPELKQCKHQCKVQRQYDEEQKEQCAKGCEKYYKEK 64

Query: 63  KEREREK----------------------------------------------------- 122
           K RE+E+                                                     
Sbjct: 65  KGREQEEEEEEEWGSGRGRGDEFSTHEPGEKRLSQCMKQCERQDGGQQKQLCRFRCQEKY 124

Query: 123 ---------GRNYESEEE--EEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSE 182
                     R+ E EEE  EE+EEE++NPYVFEDEHF  + +T +G++ VL KFT+RS+
Sbjct: 125 KKERREHSYSRDEEEEEEGDEEQEEEDENPYVFEDEHFTTRVKTEQGKVVVLPKFTKRSK 184

Query: 183 LLRGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKE-KRASFDVKCGDVF 242
           LLRG+E +R++ + ANP  FV+P H DA+ I FV+ GRGTIT I+E KR S +VK GD+ 
Sbjct: 185 LLRGLEKYRLAFLVANPQAFVVPNHMDADSIFFVSWGRGTITKIRENKRESMNVKQGDII 244

Query: 243 RVPSGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAA 302
           R+ +G PFY +N DE++KL IVKLLQP ++PGH+E+F   GGEN ESFY AFS ++LEAA
Sbjct: 245 RIRAGTPFYIVNTDENEKLYIVKLLQPVNLPGHYEVFHGPGGENPESFYRAFSREVLEAA 304

Query: 303 FKFPRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHP 362
            K PRDKLE+ F++Q  G I+KAS+EQIR++S+  E  P IWPF+ G++   FNL K+ P
Sbjct: 305 LKTPRDKLEKLFEKQDEGAIVKASKEQIRAMSRRGE-GPSIWPFT-GKSTGTFNLFKKDP 364

Query: 363 WQSNKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQ 422
            QSN +G+LFE+   ++  L++L + +++ N+TKG M  P YNSRA KIA+VV GEG  +
Sbjct: 365 SQSNNYGQLFESEFKDYPPLQELDIMVSYVNITKGGMSGPFYNSRATKIAIVVSGEGRLE 424

Query: 423 MACPHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNL 482
           +ACPHLSSS   SG      QE+ G  +Y+K+   +R   VF+VPAGHPF   AS N NL
Sbjct: 425 IACPHLSSSK-NSG------QEKSGP-SYKKLSSSIRTDSVFVVPAGHPFVTVASGNQNL 484

Query: 483 EIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPN 487
           EI+CFEVNA GN +Y LAGK+NI+  ME+ A+EL F T G EV+++F +Q+EEFFF GP 
Sbjct: 485 EILCFEVNAEGNIRYTLAGKKNIIEVMEKEAKELAFKTKGEEVDKVFGKQDEEFFFQGPK 543

BLAST of Lsi02G022710 vs. ExPASy Swiss-Prot
Match: B3STU4 (Vicilin Car i 2.0101 OS=Carya illinoinensis OX=32201 GN=pec2a1a PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.2e-101
Identity = 205/466 (43.99%), Postives = 305/466 (65.45%), Query Frame = 0

Query: 26  KDPELK--QCKHQCKVQRQFDEKQRRDCERSCDEYYKMKKEREREKGRNYESEEEE---- 85
           +DP+ +  +C+ +C+ Q Q  E+QR+ C++ C+  Y  K+++ RE G +  S   E    
Sbjct: 313 QDPQQQYHRCQRRCQTQEQSPERQRQ-CQQRCERQY--KEQQGREWGPDQASPRRESRGR 372

Query: 86  EEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELLRGIENFRVSIVEANPFTFV 145
           EEE++  NPY F  +    + E+GEG ++ L++FT+R+ELLRGIEN+RV I+EANP TFV
Sbjct: 373 EEEQQRHNPYYFHSQGLRSRHESGEGEVKYLERFTERTELLRGIENYRVVILEANPNTFV 432

Query: 146 IPTHFDAEIILFVARGRGTIT-VIKEKRASFDVKCGDVFRVPSGAPFYFINKDEHQKLKI 205
           +P H DAE ++ V RGR T+T V +E+R SF+++ GDV RVP+GA  Y IN+D +++L++
Sbjct: 433 LPYHKDAESVIVVTRGRATLTFVSQERRESFNLEYGDVIRVPAGATEYVINQDSNERLEM 492

Query: 206 VKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKFPRDKLERFFKQ--QKAGT 265
           VKLLQP + PG F  +  AG +++ES+   FS D+L AA   PRD+LERFF Q  Q+ G 
Sbjct: 493 VKLLQPVNNPGQFREYYAAGAQSTESYLRVFSNDILVAALNTPRDRLERFFDQQEQREGV 552

Query: 266 IIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQSNKFGRLFEAYPDEFSQ 325
           II+AS+E++R+LSQH     +  P+    +  P +L  Q    SN+FG+ FEA P+E  Q
Sbjct: 553 IIRASQEKLRALSQHAMSAGQ-RPWGRRSSGGPISLKSQRSSYSNQFGQFFEACPEEHRQ 612

Query: 326 LRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMACPH-LSSSSGRSGRWSE 385
           L+++ V + +A + +G+MM PHYNS+A  +  VV+G G F+MACPH +SS S       E
Sbjct: 613 LQEMDVLVNYAEIKRGAMMVPHYNSKATVVVYVVEGTGRFEMACPHDVSSQSYEYKGRRE 672

Query: 386 PEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIVCFEVNAYGNTKYLLA 445
            E+E      +QK+  RL RG +F++PAGHP ++ AS N NL +V F +N   N +  LA
Sbjct: 673 QEEEESSTGQFQKVTARLARGDIFVIPAGHPIAITASQNENLRLVGFGINGKNNQRNFLA 732

Query: 446 GKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQEQ 482
           G+ NI+N++ER A+EL FN P  E+E +F +Q E +F P   Q  +
Sbjct: 733 GQNNIINQLEREAKELSFNMPREEIEEIFERQVESYFVPMERQSRR 774

BLAST of Lsi02G022710 vs. ExPASy Swiss-Prot
Match: Q04672 (Sucrose-binding protein OS=Glycine max OX=3847 GN=SBP PE=1 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.1e-100
Identity = 219/532 (41.17%), Postives = 315/532 (59.21%), Query Frame = 0

Query: 1   MASKFNLSLLLLLFLLFVAAACLA----------SKDPELKQCKHQCKVQRQFDEKQRRD 60
           M  +  LSL +  F L    + LA           +DPEL  CKHQC+ Q+Q+ E  +R 
Sbjct: 1   MGMRTKLSLAIFFFFLLALFSNLAFGKCKETEVEEEDPELVTCKHQCQQQQQYTEGDKRV 60

Query: 61  CERSCDEYYKMKKERER-----------EKGRNYESEEEEEEEEEEKNPYVF-EDEHFEG 120
           C +SCD Y++MK+ERE+           E+ R  E E++E+ EE+++NPY+F ED+ FE 
Sbjct: 61  CLQSCDRYHRMKQEREKQIQEETREKKEEESREREEEQQEQHEEQDENPYIFEEDKDFET 120

Query: 121 KFETGEGRIRVLQKFTQRSELLRGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGT 180
           + ET  GRIRVL+KFT++S+LL+GIENFR++I+EA   TFV P HFD+E++ F  +GR  
Sbjct: 121 RVETEGGRIRVLKKFTEKSKLLQGIENFRLAILEARAHTFVSPRHFDSEVVFFNIKGRAV 180

Query: 181 ITVIKEKRA-SFDVKCGDVFRVPSGAPFYFINKDEHQKLKIVKLLQPTSV--PGHFEIFQ 240
           + ++ E       ++ GD+  +P+G P Y +N+DE+ KL +  L  P SV  PG FE F 
Sbjct: 181 LGLVSESETEKITLEPGDMIHIPAGTPLYIVNRDENDKLFLAMLHIPVSVSTPGKFEEFF 240

Query: 241 PAGGENSESFYTAFSWDLLEAAFKFPRDKLERFFKQQKAGTIIKASREQIRSLSQHEEII 300
             GG + ES  +AFSW++L+AA + P+ KLE  F QQ  G+I + SREQ+R+L+  ++  
Sbjct: 241 APGGRDPESVLSAFSWNVLQAALQTPKGKLENVFDQQNEGSIFRISREQVRALAPTKK-- 300

Query: 301 PKIWPFSEGETERPFNLLKQHPWQSNKFGRLFEAYP--DEFSQLRDLGVAIAFANLTKGS 360
              WPF  GE++  FN+  + P  SN +GRL E  P  DE S L+ L + + F N+T+ S
Sbjct: 301 SSWWPFG-GESKPQFNIFSKRPTISNGYGRLTEVGPDDDEKSWLQRLNLMLTFTNITQRS 360

Query: 361 MMAPHYNSRAMKIAVVVDGEGGFQMACPHLSSSSGRSGRWSEPEQERKGERTYQKIRGRL 420
           M   HYNS A KIA+V+DG G  Q++CPH+SS S  S       +  K   +Y +I   L
Sbjct: 361 MSTIHYNSHATKIALVIDGRGHLQISCPHMSSRSSHS-------KHDKSSPSYHRISSDL 420

Query: 421 RRGVVFIVPAGHPFSVFASPNHNLEIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGF 480
           + G+VF+VP GHPF   AS   NL ++CFEVNA  N K+  AGK+NIV+ ++ VA+EL F
Sbjct: 421 KPGMVFVVPPGHPFVTIASNKENLLMICFEVNARDNKKFTFAGKDNIVSSLDNVAKELAF 480

Query: 481 NTPGREVERMF-------------------RQQEEEFFFPGPNQQEQEWAEA 487
           N P   V  +F                   + ++E FFFP    +E+    A
Sbjct: 481 NYPSEMVNGVFLLQRFLERKLIGRLYHLPHKDRKESFFFPFELPREERGRRA 522

BLAST of Lsi02G022710 vs. ExPASy TrEMBL
Match: A0A1S3BS82 (sucrose-binding protein-like OS=Cucumis melo OX=3656 GN=LOC103492635 PE=3 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.5e-243
Identity = 429/489 (87.73%), Postives = 455/489 (93.05%), Query Frame = 0

Query: 4   KFNLSL-LLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYKMK 63
           K NL+L LLLLFL+      LASKDPELKQCKHQCKVQRQFDE+Q+RDCERSCDEYYKMK
Sbjct: 31  KINLNLHLLLLFLITALCLALASKDPELKQCKHQCKVQRQFDEQQKRDCERSCDEYYKMK 90

Query: 64  KEREREKGRNYES-----EEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRS 123
           K    EKGRNYES     EEEEEEEEEE+NPYVF+DEHF GK ETGEG+I+VLQKFTQRS
Sbjct: 91  K----EKGRNYESEEEEEEEEEEEEEEEENPYVFDDEHFVGKVETGEGKIKVLQKFTQRS 150

Query: 124 ELLRGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVF 183
           +LLRGIENFRVSIVEANP TFV+PTHFDAEII+FVA+GRGTITVIKEKR SFD+KCGDVF
Sbjct: 151 QLLRGIENFRVSIVEANPSTFVVPTHFDAEIIMFVAQGRGTITVIKEKRGSFDLKCGDVF 210

Query: 184 RVPSGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAA 243
           R+PSGAPFYFINKDEHQKLKIVKLLQ TSVPGHF+ FQPAGGEN ESFYTAFSWDLLEAA
Sbjct: 211 RIPSGAPFYFINKDEHQKLKIVKLLQSTSVPGHFQTFQPAGGENPESFYTAFSWDLLEAA 270

Query: 244 FKFPRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHP 303
           FK PRDKLERFFKQQ+ GTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHP
Sbjct: 271 FKIPRDKLERFFKQQRPGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHP 330

Query: 304 WQSNKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQ 363
           WQSNKFGRLFEAYPDEFSQLRDLGVAIAFAN+TKGSMM PHYNS++MKIAVV+DGEGGFQ
Sbjct: 331 WQSNKFGRLFEAYPDEFSQLRDLGVAIAFANITKGSMMTPHYNSKSMKIAVVLDGEGGFQ 390

Query: 364 MACPHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNL 423
           MACPHLSSSSGRSGRWSE E+ RKGERTYQKIRGRL RGVVF+VPAGHPFSVFASPNH+L
Sbjct: 391 MACPHLSSSSGRSGRWSEREEGRKGERTYQKIRGRLSRGVVFVVPAGHPFSVFASPNHSL 450

Query: 424 EIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPN 483
           +IVCFEVNAYGNTKY LAGKENIVNKME +ARELGFNTPGREVERMF+QQEEEFFFPGPN
Sbjct: 451 QIVCFEVNAYGNTKYFLAGKENIVNKMESIARELGFNTPGREVERMFKQQEEEFFFPGPN 510

Query: 484 QQEQEWAEA 487
           QQE EWA A
Sbjct: 511 QQEHEWASA 515

BLAST of Lsi02G022710 vs. ExPASy TrEMBL
Match: A0A0A0KAE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G380130 PE=4 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 4.2e-241
Identity = 423/484 (87.40%), Postives = 454/484 (93.80%), Query Frame = 0

Query: 4   KFNLSL-LLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYKMK 63
           K NL+L LLLLFL+      LASKDPELKQCKHQCKVQRQFDE+Q+RDCERSCDEYYKMK
Sbjct: 6   KINLNLHLLLLFLITAVCLALASKDPELKQCKHQCKVQRQFDEQQKRDCERSCDEYYKMK 65

Query: 64  KEREREKGRNYESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELLRG 123
           K    EKGRNYESEEEEEEEEE +NPYVF+DEHF G+ ETGEG+I+VLQKFTQRS LLRG
Sbjct: 66  K----EKGRNYESEEEEEEEEEVENPYVFDDEHFVGQIETGEGKIKVLQKFTQRSHLLRG 125

Query: 124 IENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVFRVPSG 183
           IENFRVSIVEANP TFV+PTHFDAEIILFVA+GRGTITVIKEKR SF++KCGDVFR+PSG
Sbjct: 126 IENFRVSIVEANPSTFVVPTHFDAEIILFVAQGRGTITVIKEKRGSFELKCGDVFRIPSG 185

Query: 184 APFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKFPR 243
           APFYFINKDEH+KLKIVKLLQ TSVPGHF+ FQPAGGEN ESFYTAFSWDLLEAAFK PR
Sbjct: 186 APFYFINKDEHRKLKIVKLLQSTSVPGHFQTFQPAGGENPESFYTAFSWDLLEAAFKIPR 245

Query: 244 DKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQSNK 303
           DKLERFFKQQK GTIIKASREQIRSLS+HEEIIPKIWPFSEGETERPFNLLKQHP QSNK
Sbjct: 246 DKLERFFKQQKPGTIIKASREQIRSLSRHEEIIPKIWPFSEGETERPFNLLKQHPCQSNK 305

Query: 304 FGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMACPH 363
           FGRLFEAYPDEFSQLRDLGVAIAFAN+T+GSM+APHYNS++MKIAVV+DG+GGFQMACPH
Sbjct: 306 FGRLFEAYPDEFSQLRDLGVAIAFANITEGSMVAPHYNSKSMKIAVVLDGQGGFQMACPH 365

Query: 364 LSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIVCF 423
           LSSSS RSGRWSE E+ERKGERTYQKIRGRL RGVVF+VPAGHPFSVFASPNH+L+IVCF
Sbjct: 366 LSSSSRRSGRWSEREEERKGERTYQKIRGRLSRGVVFVVPAGHPFSVFASPNHSLQIVCF 425

Query: 424 EVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQEQE 483
           EVNAYGNTKY LAGKENIVNKME +ARELGFNTPGREVERMF+QQEEEFFFPGPNQQE E
Sbjct: 426 EVNAYGNTKYFLAGKENIVNKMESIARELGFNTPGREVERMFKQQEEEFFFPGPNQQEHE 485

Query: 484 WAEA 487
           WA+A
Sbjct: 486 WADA 485

BLAST of Lsi02G022710 vs. ExPASy TrEMBL
Match: A0A6J1JS85 (sucrose-binding protein-like OS=Cucurbita maxima OX=3661 GN=LOC111488449 PE=4 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.5e-227
Identity = 407/486 (83.74%), Postives = 436/486 (89.71%), Query Frame = 0

Query: 1   MASKFNLSLLLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYK 60
           MASK    + LLL LL V   CLA KDPEL+QCKHQC+VQ++FDEKQ+RDCE+ CDEY+K
Sbjct: 1   MASK----IYLLLLLLSVTVGCLAFKDPELEQCKHQCRVQQKFDEKQKRDCEKMCDEYHK 60

Query: 61  MKKEREREKGRNYESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELL 120
           MKKERERE+GR YESEEE EEE  E NPYVF+DEHFE + ETGEGRIRVLQ+FTQRSELL
Sbjct: 61  MKKEREREEGRYYESEEEREEEYGESNPYVFDDEHFEERVETGEGRIRVLQRFTQRSELL 120

Query: 121 RGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVFRVP 180
           RGIEN+RVS+VE NP TFV P+HFDAEIILFVA GRGTITVIKEKR SFDVKCGDVFRVP
Sbjct: 121 RGIENYRVSLVEVNPSTFVSPSHFDAEIILFVAEGRGTITVIKEKRQSFDVKCGDVFRVP 180

Query: 181 SGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKF 240
           SGAPFYFINKDEHQKLKIVKLLQ TSVPG FEIFQP GGEN ESFYTAFSWDLLEAAFK 
Sbjct: 181 SGAPFYFINKDEHQKLKIVKLLQSTSVPGSFEIFQP-GGENPESFYTAFSWDLLEAAFKI 240

Query: 241 PRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQS 300
           P+DKL+RFF+QQK GTIIKASREQIRSLS+ EE IP+IWPFSEGETERPFNLLKQ PWQS
Sbjct: 241 PKDKLKRFFEQQKEGTIIKASREQIRSLSRPEEFIPRIWPFSEGETERPFNLLKQDPWQS 300

Query: 301 NKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMAC 360
           NKFGRLFEA P EF QLRDL VA+AFAN+TKGSMM PHY S+A KIAVVVDGEGGFQMAC
Sbjct: 301 NKFGRLFEADPGEFRQLRDLNVAVAFANMTKGSMMTPHYYSKATKIAVVVDGEGGFQMAC 360

Query: 361 PHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIV 420
           PHLSSSSGR GRWSE E +R GE TYQKIRGRLRRGVVFI+PAGHPFSVFASPNHNL+IV
Sbjct: 361 PHLSSSSGRGGRWSEREHKRTGEGTYQKIRGRLRRGVVFIIPAGHPFSVFASPNHNLQIV 420

Query: 421 CFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQE 480
           CFEVNAYGNTKYLLAGK+NIVNKME VARELGFN PGREVERMFRQQEEEFF PGP+QQE
Sbjct: 421 CFEVNAYGNTKYLLAGKDNIVNKMESVARELGFNIPGREVERMFRQQEEEFFLPGPDQQE 480

Query: 481 QEWAEA 487
            EW +A
Sbjct: 481 HEWTDA 481

BLAST of Lsi02G022710 vs. ExPASy TrEMBL
Match: A0A6J1GPW2 (sucrose-binding protein-like OS=Cucurbita moschata OX=3662 GN=LOC111456032 PE=4 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.5e-227
Identity = 408/486 (83.95%), Postives = 437/486 (89.92%), Query Frame = 0

Query: 1   MASKFNLSLLLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYK 60
           MASK    + LLL LL V   CLASKDPEL+QCKHQC+VQ++FDEKQ+RDCE+ CDEY+K
Sbjct: 1   MASK----IYLLLLLLSVTVGCLASKDPELEQCKHQCRVQQKFDEKQKRDCEKMCDEYHK 60

Query: 61  MKKEREREKGRNYESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELL 120
           MKKERERE+GR YESEEE EEE  E NPYVF+DEHFE + ETGEGRIRVLQ+FTQRSELL
Sbjct: 61  MKKEREREEGRYYESEEEREEEYGESNPYVFDDEHFEERVETGEGRIRVLQRFTQRSELL 120

Query: 121 RGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVFRVP 180
           R IEN+RVS+VEANP TFV P+HFDAEIILFVA GRGTITVIKEKR SFDVKCGDVF VP
Sbjct: 121 RVIENYRVSLVEANPSTFVSPSHFDAEIILFVAEGRGTITVIKEKRQSFDVKCGDVFIVP 180

Query: 181 SGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKF 240
           SGAPFYFINKDEHQKLKIVKLLQ TSVPG FEIFQP GGEN ESFYTAFSWDLLEAAFK 
Sbjct: 181 SGAPFYFINKDEHQKLKIVKLLQSTSVPGSFEIFQP-GGENPESFYTAFSWDLLEAAFKI 240

Query: 241 PRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQS 300
           P+DKL+RFF+QQK GTIIKASREQIRSLS+ EE IP+IWPFSEGETERPFNLLKQ PWQS
Sbjct: 241 PKDKLKRFFEQQKEGTIIKASREQIRSLSRPEEFIPRIWPFSEGETERPFNLLKQDPWQS 300

Query: 301 NKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMAC 360
           NKFGRLFEA P EF QLRDL VA+AFAN+TKGSMM PHY S+A KIAVVVDGEGGFQMAC
Sbjct: 301 NKFGRLFEADPGEFRQLRDLNVAVAFANMTKGSMMTPHYYSKATKIAVVVDGEGGFQMAC 360

Query: 361 PHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIV 420
           PHLSSSSGR GRWSE E +R GE TYQKIRGRLRRGVVFI+PAGHPFSVFASPNHNL+IV
Sbjct: 361 PHLSSSSGRGGRWSEREHKRMGEGTYQKIRGRLRRGVVFIIPAGHPFSVFASPNHNLQIV 420

Query: 421 CFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQE 480
           CFEVNAYGNTKYLLAGK+NIVNKME VARELGFNTPGREVER+FRQQEEEFF PGPNQQE
Sbjct: 421 CFEVNAYGNTKYLLAGKDNIVNKMESVARELGFNTPGREVERIFRQQEEEFFLPGPNQQE 480

Query: 481 QEWAEA 487
            EW +A
Sbjct: 481 HEWTDA 481

BLAST of Lsi02G022710 vs. ExPASy TrEMBL
Match: A0A6J1D921 (sucrose-binding protein-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018489 PE=4 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.5e-177
Identity = 329/488 (67.42%), Postives = 388/488 (79.51%), Query Frame = 0

Query: 1   MASKFNLSLLLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDE--Y 60
           MA K  LSL LLL LL ++ A LA KDP+LKQC+ QCK++ +  E+QRRDCE+ C+E   
Sbjct: 1   MAPKIKLSLPLLL-LLVLSVAVLAGKDPDLKQCRSQCKLKLEIAERQRRDCEKRCEETKK 60

Query: 61  YKMKKEREREKGRNY-------ESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQ 120
            K KKE+E E GR         E  E EE E+EE+NPYVF++EHF+ + ET EG +RVL 
Sbjct: 61  KKKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLP 120

Query: 121 KFTQRSELLRGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDV 180
           KFTQ+S+LLRGIENFR+SI+EAN  TFV P+HFDAEIILFVA+GR TITVI+E+RASF++
Sbjct: 121 KFTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATITVIRERRASFEL 180

Query: 181 KCGDVFRVPSGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSW 240
           K GDVFRVPSGAPFY  NKDE +KL+I KLLQ TS+PG FE+F   GG+N ESFYTAFSW
Sbjct: 181 KPGDVFRVPSGAPFYLTNKDEREKLRIFKLLQATSIPGQFEVFHGPGGDNPESFYTAFSW 240

Query: 241 DLLEAAFKFPRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFN 300
           +LLEAA K PRD+LERFFKQQK G I++ASREQI+SLSQ EEIIP+IWPFSEG+TERPFN
Sbjct: 241 ELLEAALKVPRDELERFFKQQKGGAIMRASREQIQSLSQQEEIIPRIWPFSEGKTERPFN 300

Query: 301 LLKQHPWQSNKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVD 360
           L KQ PWQSNKFG  F A+P EFSQL+DLG+A++FAN T+GSMMAPHYNS+A+K+ VVVD
Sbjct: 301 LFKQSPWQSNKFGHFFIAHPHEFSQLQDLGIAVSFANFTEGSMMAPHYNSKAIKVGVVVD 360

Query: 361 GEGGFQMACPHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFA 420
           GEG FQMACPH     G   R         GE +YQ+IRG LRR +V I PAGHPFS+ A
Sbjct: 361 GEGEFQMACPHRGRHHGGGHR---------GESSYQRIRGVLRRDMVIIAPAGHPFSIIA 420

Query: 421 SPNHNLEIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEF 480
           +PNH L+IV FE+NA GN KYLLAGKENI+NKMER+ARELGF T G+ V RMFRQQEEEF
Sbjct: 421 APNHRLQIVFFEINADGNIKYLLAGKENIINKMERIARELGFGTSGKAVGRMFRQQEEEF 478

BLAST of Lsi02G022710 vs. NCBI nr
Match: XP_038898840.1 (vicilin Jug r 6.0101-like [Benincasa hispida])

HSP 1 Score: 894.0 bits (2309), Expect = 5.6e-256
Identity = 449/486 (92.39%), Postives = 468/486 (96.30%), Query Frame = 0

Query: 1   MASKFNLSLLLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYK 60
           MASKF L   LLLFLLF+ AACLASKDPELKQCKHQCKVQRQFDE+QRRDCERSCDEYYK
Sbjct: 1   MASKFKLP--LLLFLLFLTAACLASKDPELKQCKHQCKVQRQFDEQQRRDCERSCDEYYK 60

Query: 61  MKKEREREKGRNYESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELL 120
           MKKERER KGRNYE EEE ++++EEKNPYVFED+HFEGK ETGEGRIRVLQKFTQRSELL
Sbjct: 61  MKKERERGKGRNYEREEEGDDDDEEKNPYVFEDKHFEGKVETGEGRIRVLQKFTQRSELL 120

Query: 121 RGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVFRVP 180
           RGIENFRVSIVEANP TFVIPTHFDAEI+LFVA+GRGTITVIKEKR SFDVKCGDVFR+P
Sbjct: 121 RGIENFRVSIVEANPSTFVIPTHFDAEILLFVAQGRGTITVIKEKRGSFDVKCGDVFRIP 180

Query: 181 SGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKF 240
           SGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFK 
Sbjct: 181 SGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKI 240

Query: 241 PRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQS 300
           PR+KLERFF QQK GTIIKASREQIRSLSQHEEIIP+IWPFSEGETERPFNLLKQHPWQS
Sbjct: 241 PREKLERFFDQQKPGTIIKASREQIRSLSQHEEIIPRIWPFSEGETERPFNLLKQHPWQS 300

Query: 301 NKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMAC 360
           NKFGRLFEAYPDEFSQLRDLGVA+AFAN+TKGSMMAPHYNS+AMKIAVVV+GEGGFQMAC
Sbjct: 301 NKFGRLFEAYPDEFSQLRDLGVAVAFANMTKGSMMAPHYNSKAMKIAVVVEGEGGFQMAC 360

Query: 361 PHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIV 420
           PHLSSSSGRSGRWSE EQERKG RTYQKIRG+LRRGVVFIVPAGHPFSVFASPNH L+IV
Sbjct: 361 PHLSSSSGRSGRWSEREQERKGGRTYQKIRGQLRRGVVFIVPAGHPFSVFASPNHGLQIV 420

Query: 421 CFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQE 480
           CFEVNAYGNTKYLLAGK+NIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQE
Sbjct: 421 CFEVNAYGNTKYLLAGKDNIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQE 480

Query: 481 QEWAEA 487
            EWAEA
Sbjct: 481 HEWAEA 484

BLAST of Lsi02G022710 vs. NCBI nr
Match: XP_008451293.1 (PREDICTED: sucrose-binding protein-like [Cucumis melo])

HSP 1 Score: 851.7 bits (2199), Expect = 3.2e-243
Identity = 429/489 (87.73%), Postives = 455/489 (93.05%), Query Frame = 0

Query: 4   KFNLSL-LLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYKMK 63
           K NL+L LLLLFL+      LASKDPELKQCKHQCKVQRQFDE+Q+RDCERSCDEYYKMK
Sbjct: 31  KINLNLHLLLLFLITALCLALASKDPELKQCKHQCKVQRQFDEQQKRDCERSCDEYYKMK 90

Query: 64  KEREREKGRNYES-----EEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRS 123
           K    EKGRNYES     EEEEEEEEEE+NPYVF+DEHF GK ETGEG+I+VLQKFTQRS
Sbjct: 91  K----EKGRNYESEEEEEEEEEEEEEEEENPYVFDDEHFVGKVETGEGKIKVLQKFTQRS 150

Query: 124 ELLRGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVF 183
           +LLRGIENFRVSIVEANP TFV+PTHFDAEII+FVA+GRGTITVIKEKR SFD+KCGDVF
Sbjct: 151 QLLRGIENFRVSIVEANPSTFVVPTHFDAEIIMFVAQGRGTITVIKEKRGSFDLKCGDVF 210

Query: 184 RVPSGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAA 243
           R+PSGAPFYFINKDEHQKLKIVKLLQ TSVPGHF+ FQPAGGEN ESFYTAFSWDLLEAA
Sbjct: 211 RIPSGAPFYFINKDEHQKLKIVKLLQSTSVPGHFQTFQPAGGENPESFYTAFSWDLLEAA 270

Query: 244 FKFPRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHP 303
           FK PRDKLERFFKQQ+ GTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHP
Sbjct: 271 FKIPRDKLERFFKQQRPGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHP 330

Query: 304 WQSNKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQ 363
           WQSNKFGRLFEAYPDEFSQLRDLGVAIAFAN+TKGSMM PHYNS++MKIAVV+DGEGGFQ
Sbjct: 331 WQSNKFGRLFEAYPDEFSQLRDLGVAIAFANITKGSMMTPHYNSKSMKIAVVLDGEGGFQ 390

Query: 364 MACPHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNL 423
           MACPHLSSSSGRSGRWSE E+ RKGERTYQKIRGRL RGVVF+VPAGHPFSVFASPNH+L
Sbjct: 391 MACPHLSSSSGRSGRWSEREEGRKGERTYQKIRGRLSRGVVFVVPAGHPFSVFASPNHSL 450

Query: 424 EIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPN 483
           +IVCFEVNAYGNTKY LAGKENIVNKME +ARELGFNTPGREVERMF+QQEEEFFFPGPN
Sbjct: 451 QIVCFEVNAYGNTKYFLAGKENIVNKMESIARELGFNTPGREVERMFKQQEEEFFFPGPN 510

Query: 484 QQEQEWAEA 487
           QQE EWA A
Sbjct: 511 QQEHEWASA 515

BLAST of Lsi02G022710 vs. NCBI nr
Match: XP_011659275.1 (sucrose-binding protein [Cucumis sativus] >KAE8646304.1 hypothetical protein Csa_016372 [Cucumis sativus])

HSP 1 Score: 843.6 bits (2178), Expect = 8.7e-241
Identity = 423/484 (87.40%), Postives = 454/484 (93.80%), Query Frame = 0

Query: 4   KFNLSL-LLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYKMK 63
           K NL+L LLLLFL+      LASKDPELKQCKHQCKVQRQFDE+Q+RDCERSCDEYYKMK
Sbjct: 31  KINLNLHLLLLFLITAVCLALASKDPELKQCKHQCKVQRQFDEQQKRDCERSCDEYYKMK 90

Query: 64  KEREREKGRNYESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELLRG 123
           K    EKGRNYESEEEEEEEEE +NPYVF+DEHF G+ ETGEG+I+VLQKFTQRS LLRG
Sbjct: 91  K----EKGRNYESEEEEEEEEEVENPYVFDDEHFVGQIETGEGKIKVLQKFTQRSHLLRG 150

Query: 124 IENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVFRVPSG 183
           IENFRVSIVEANP TFV+PTHFDAEIILFVA+GRGTITVIKEKR SF++KCGDVFR+PSG
Sbjct: 151 IENFRVSIVEANPSTFVVPTHFDAEIILFVAQGRGTITVIKEKRGSFELKCGDVFRIPSG 210

Query: 184 APFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKFPR 243
           APFYFINKDEH+KLKIVKLLQ TSVPGHF+ FQPAGGEN ESFYTAFSWDLLEAAFK PR
Sbjct: 211 APFYFINKDEHRKLKIVKLLQSTSVPGHFQTFQPAGGENPESFYTAFSWDLLEAAFKIPR 270

Query: 244 DKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQSNK 303
           DKLERFFKQQK GTIIKASREQIRSLS+HEEIIPKIWPFSEGETERPFNLLKQHP QSNK
Sbjct: 271 DKLERFFKQQKPGTIIKASREQIRSLSRHEEIIPKIWPFSEGETERPFNLLKQHPCQSNK 330

Query: 304 FGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMACPH 363
           FGRLFEAYPDEFSQLRDLGVAIAFAN+T+GSM+APHYNS++MKIAVV+DG+GGFQMACPH
Sbjct: 331 FGRLFEAYPDEFSQLRDLGVAIAFANITEGSMVAPHYNSKSMKIAVVLDGQGGFQMACPH 390

Query: 364 LSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIVCF 423
           LSSSS RSGRWSE E+ERKGERTYQKIRGRL RGVVF+VPAGHPFSVFASPNH+L+IVCF
Sbjct: 391 LSSSSRRSGRWSEREEERKGERTYQKIRGRLSRGVVFVVPAGHPFSVFASPNHSLQIVCF 450

Query: 424 EVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQEQE 483
           EVNAYGNTKY LAGKENIVNKME +ARELGFNTPGREVERMF+QQEEEFFFPGPNQQE E
Sbjct: 451 EVNAYGNTKYFLAGKENIVNKMESIARELGFNTPGREVERMFKQQEEEFFFPGPNQQEHE 510

Query: 484 WAEA 487
           WA+A
Sbjct: 511 WADA 510

BLAST of Lsi02G022710 vs. NCBI nr
Match: KAG6575924.1 (Sucrose-binding protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014457.1 Sucrose-binding protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 798.9 bits (2062), Expect = 2.4e-227
Identity = 408/486 (83.95%), Postives = 437/486 (89.92%), Query Frame = 0

Query: 1   MASKFNLSLLLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYK 60
           MASK    + LLL LL V   CLASKDPEL+QCKHQC+VQ++FDEKQ+RDCE+ CDEY+K
Sbjct: 1   MASK----IYLLLLLLSVTVGCLASKDPELEQCKHQCRVQQKFDEKQKRDCEKMCDEYHK 60

Query: 61  MKKEREREKGRNYESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELL 120
           MK+ERERE+GR YESEEE EEE  E NPYVF+DEHFE + ETGEGRIRVLQ+FTQRSELL
Sbjct: 61  MKREREREEGRYYESEEEREEEYGESNPYVFDDEHFEERVETGEGRIRVLQRFTQRSELL 120

Query: 121 RGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVFRVP 180
           R IEN+RVS+VEANP TFV P+HFDAEIILFVA GRGTITVIKEKR SFDVKCGDVF VP
Sbjct: 121 RVIENYRVSLVEANPSTFVSPSHFDAEIILFVAEGRGTITVIKEKRQSFDVKCGDVFIVP 180

Query: 181 SGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKF 240
           SGAPFYFINKDEHQKLKIVKLLQ TSVPG FEIFQP GGEN ESFYTAFSWDLLEAAFK 
Sbjct: 181 SGAPFYFINKDEHQKLKIVKLLQSTSVPGSFEIFQP-GGENPESFYTAFSWDLLEAAFKI 240

Query: 241 PRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQS 300
           P+DKL+RFF+QQK GTIIKASREQIRSLS+ EE IP+IWPFSEGETERPFNLLKQ PWQS
Sbjct: 241 PKDKLKRFFEQQKEGTIIKASREQIRSLSRPEEFIPRIWPFSEGETERPFNLLKQDPWQS 300

Query: 301 NKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMAC 360
           NKFGRLFEA P EF QLRDL VA+AFAN+TKGSMM PHY S+A KIAVVVDGEGGFQMAC
Sbjct: 301 NKFGRLFEADPGEFRQLRDLNVAVAFANMTKGSMMTPHYYSKATKIAVVVDGEGGFQMAC 360

Query: 361 PHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIV 420
           PHLSSSSGR GRWSE E +R GE TYQKIRGRLRRGVVFI+PAGHPFSVFASPNHNL+IV
Sbjct: 361 PHLSSSSGRGGRWSEREHKRTGEGTYQKIRGRLRRGVVFIIPAGHPFSVFASPNHNLQIV 420

Query: 421 CFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQE 480
           CFEVNAYGNTKYLLAGK+NIVNKME VARELGFNTPGREVERMFRQQEEEFF PGPNQQE
Sbjct: 421 CFEVNAYGNTKYLLAGKDNIVNKMESVARELGFNTPGREVERMFRQQEEEFFLPGPNQQE 480

Query: 481 QEWAEA 487
            EW +A
Sbjct: 481 HEWTDA 481

BLAST of Lsi02G022710 vs. NCBI nr
Match: XP_022953499.1 (sucrose-binding protein-like [Cucurbita moschata])

HSP 1 Score: 798.5 bits (2061), Expect = 3.2e-227
Identity = 408/486 (83.95%), Postives = 437/486 (89.92%), Query Frame = 0

Query: 1   MASKFNLSLLLLLFLLFVAAACLASKDPELKQCKHQCKVQRQFDEKQRRDCERSCDEYYK 60
           MASK    + LLL LL V   CLASKDPEL+QCKHQC+VQ++FDEKQ+RDCE+ CDEY+K
Sbjct: 1   MASK----IYLLLLLLSVTVGCLASKDPELEQCKHQCRVQQKFDEKQKRDCEKMCDEYHK 60

Query: 61  MKKEREREKGRNYESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELL 120
           MKKERERE+GR YESEEE EEE  E NPYVF+DEHFE + ETGEGRIRVLQ+FTQRSELL
Sbjct: 61  MKKEREREEGRYYESEEEREEEYGESNPYVFDDEHFEERVETGEGRIRVLQRFTQRSELL 120

Query: 121 RGIENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVFRVP 180
           R IEN+RVS+VEANP TFV P+HFDAEIILFVA GRGTITVIKEKR SFDVKCGDVF VP
Sbjct: 121 RVIENYRVSLVEANPSTFVSPSHFDAEIILFVAEGRGTITVIKEKRQSFDVKCGDVFIVP 180

Query: 181 SGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKF 240
           SGAPFYFINKDEHQKLKIVKLLQ TSVPG FEIFQP GGEN ESFYTAFSWDLLEAAFK 
Sbjct: 181 SGAPFYFINKDEHQKLKIVKLLQSTSVPGSFEIFQP-GGENPESFYTAFSWDLLEAAFKI 240

Query: 241 PRDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETERPFNLLKQHPWQS 300
           P+DKL+RFF+QQK GTIIKASREQIRSLS+ EE IP+IWPFSEGETERPFNLLKQ PWQS
Sbjct: 241 PKDKLKRFFEQQKEGTIIKASREQIRSLSRPEEFIPRIWPFSEGETERPFNLLKQDPWQS 300

Query: 301 NKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMAC 360
           NKFGRLFEA P EF QLRDL VA+AFAN+TKGSMM PHY S+A KIAVVVDGEGGFQMAC
Sbjct: 301 NKFGRLFEADPGEFRQLRDLNVAVAFANMTKGSMMTPHYYSKATKIAVVVDGEGGFQMAC 360

Query: 361 PHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIV 420
           PHLSSSSGR GRWSE E +R GE TYQKIRGRLRRGVVFI+PAGHPFSVFASPNHNL+IV
Sbjct: 361 PHLSSSSGRGGRWSEREHKRMGEGTYQKIRGRLRRGVVFIIPAGHPFSVFASPNHNLQIV 420

Query: 421 CFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEEEFFFPGPNQQE 480
           CFEVNAYGNTKYLLAGK+NIVNKME VARELGFNTPGREVER+FRQQEEEFF PGPNQQE
Sbjct: 421 CFEVNAYGNTKYLLAGKDNIVNKMESVARELGFNTPGREVERIFRQQEEEFFLPGPNQQE 480

Query: 481 QEWAEA 487
            EW +A
Sbjct: 481 HEWTDA 481

BLAST of Lsi02G022710 vs. TAIR 10
Match: AT3G22640.1 (cupin family protein )

HSP 1 Score: 227.6 bits (579), Expect = 2.1e-59
Identity = 142/432 (32.87%), Postives = 229/432 (53.01%), Query Frame = 0

Query: 64  EREREKGRNYESEEEEEEEEEEKNPYVFEDEHFEGKFETGEGRIRVLQKFTQRS-ELLRG 123
           E+E  + R      E  EEE   +PY F    F   F++ EG +RVL KFT+ +  L RG
Sbjct: 39  EQEGPRRRPGGGSGEGWEEESTNHPYHFRKRSFSDWFQSKEGFVRVLPKFTKHAPALFRG 98

Query: 124 IENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEK-RASFDVKCGDVFRVPS 183
           IEN+R S+VE  P TF +P H DA+ +  V +G+G I  + +K + SF +  GDV R+PS
Sbjct: 99  IENYRFSLVEMEPTTFFVPHHLDADAVFIVLQGKGVIEFVTDKTKESFHITKGDVVRIPS 158

Query: 184 GAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWDLLEAAFKFP 243
           G   +  N ++   L++ ++  P + PG+++ + PA  +  +S++  F+ ++L  +F  P
Sbjct: 159 GVTNFITNTNQTVPLRLAQITVPVNNPGNYKDYFPAASQFQQSYFNGFTKEVLSTSFNVP 218

Query: 244 RDKLERFFKQQK---AGTIIKASREQIRSLSQHEEIIPKIWPFSEGETER---------P 303
            + L R   + K    G I + S +QI+ L++H    P     ++ E E          P
Sbjct: 219 EELLGRLVTRSKEIGQGIIRRISPDQIKELAEH-ATSPSNKHKAKKEKEEDKDLRTLWTP 278

Query: 304 FNLLKQHPWQSNKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVV 363
           FNL    P  SN FG   EA+P  ++QL+DL +A A+AN+T+GS+  PH+NS+   +  V
Sbjct: 279 FNLFAIDPIYSNDFGHFHEAHPKNYNQLQDLHIAAAWANMTQGSLFLPHFNSKTTFVTFV 338

Query: 364 VDGEGGFQMACPHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVPAGHPFSV 423
            +G   F+MA P+      +       E+E        K+  R+ +G VFIVPAGHPF++
Sbjct: 339 ENGCARFEMATPYKFQRGQQQWPGQGQEEEEDMSENVHKVVSRVCKGEVFIVPAGHPFTI 398

Query: 424 FASPNHNLEIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVERMFRQQEE 482
             S + +   V F + A  + +  LAG+EN+++ +   A  + F    +  E++F  Q  
Sbjct: 399 L-SQDQDFIAVGFGIYATNSKRTFLAGEENLLSNLNPAATRVTFGVGSKVAEKLFTSQNY 458

BLAST of Lsi02G022710 vs. TAIR 10
Match: AT2G28490.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 5.2e-26
Identity = 92/400 (23.00%), Postives = 157/400 (39.25%), Query Frame = 0

Query: 128 VSIVEANPFTFVIPTHFDAEIILFVARGRGTITVI-KEKRASFDVKCGDVFRVPSGAPFY 187
           +  +   P T  +P + D+ +++F+ +G  T+ VI K++     +K GD++ +P+G+ FY
Sbjct: 119 IGFLTMEPKTLFVPQYLDSSLLIFIRQGEATLGVICKDEFGERKLKAGDIYWIPAGSVFY 178

Query: 188 FINKDEHQKLKIVKLLQPTSVPGHFEIFQPAG-GENSESFYTAFSWDLLEAAFKFPRDKL 247
             N    Q+L ++  + PT   G FE FQP   G    S    F    L +AF     +L
Sbjct: 179 LHNTGLGQRLHVICSIDPTQSLG-FETFQPFYIGGGPSSVLAGFDPHTLTSAFNVSLPEL 238

Query: 248 ERFFKQQKAGTII-----------------------KASREQIRSLSQHEEIIPKIWPFS 307
           ++    Q  G I+                       +   +Q++ L + ++  P+   +S
Sbjct: 239 QQMMMSQFRGPIVYVTEGPQPQPQSTVWTQFLGLRGEEKHKQLKKLLETKQGSPQDQQYS 298

Query: 308 EG------------------------ETERPFNLL--KQHPWQSNKFGRLFEAYPDEFSQ 367
            G                        E E  +N+   K  P   NK+G       D++  
Sbjct: 299 SGWSWRNIVRSILDLTEEKNKGSGSSECEDSYNIYDKKDKPSFDNKYGWSIALDYDDYKP 358

Query: 368 LRDLGVAIAFANLTKGSMMAPHYNSRAMKIAVVVDGEGGFQMACPHLSSSSGRSGRWSEP 427
           L+  G+ +   NLT G+MMAPH N  A +  +V+ G G  Q+  P+ +S+          
Sbjct: 359 LKHSGIGVYLVNLTAGAMMAPHMNPTATEYGIVLAGSGEIQVVFPNGTSA---------- 418

Query: 428 EQERKGERTYQKIRGRLRRGVVFIVPAGHPFSVFASPNHNLEIVCFEVNAYGNTKYLLAG 477
                       +  R+  G VF +P    F   AS     E V F  +A+ N    L G
Sbjct: 419 ------------MNTRVSVGDVFWIPRYFAFCQIASRTGPFEFVGFTTSAHKNRPQFLVG 478

BLAST of Lsi02G022710 vs. TAIR 10
Match: AT2G18540.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 1.0e-21
Identity = 85/371 (22.91%), Postives = 155/371 (41.78%), Query Frame = 0

Query: 124 ENFRVSIVEANPFTFVIPTHFDAEIILFVARGRGTITVI-KEKRASFDVKCGDVFRVPSG 183
           + + +  +   P   ++P    ++++ FV  G G +  I +E     +++ GDVFR+ SG
Sbjct: 68  DGYHIQFITLEPNALLLPLLLHSDMVFFVHTGTGILNWIDEESERKLELRRGDVFRLRSG 127

Query: 184 APFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENS--ESFYTAFSWDLLEAAFKF 243
             FY      ++KL++  +           +  P  G  S        F    L +AF  
Sbjct: 128 TVFYV---HSNEKLRVYAIFNVGKC-----LNDPCLGAYSSVRDLLLGFDDRTLRSAFAV 187

Query: 244 PRDKLERFFKQQKAGTIIKA---SREQIRSLSQHEEIIPKIWPFSEGETE---------- 303
           P D L +     K   I+ A   +R Q     + +  + +++   E  T+          
Sbjct: 188 PEDILRKIRDATKPPLIVNALPRNRTQGLEEDKWQSRLVRLFVSVEDVTDHLAMKPIVDT 247

Query: 304 -----RPFNLLKQHPWQSNKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNSR 363
                R FN+ ++ P   N  GR       +   L+     +   NLTKGSM+ PH+N  
Sbjct: 248 NKKKSRTFNVFEEDPDFENNNGRSIVVDEKDLDALKGSRFGVFMVNLTKGSMIGPHWNPS 307

Query: 364 AMKIAVVVDGEGGFQMACPHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIVP 423
           A +I++V++GEG  ++     S SS ++ R SE     +G+              VF+VP
Sbjct: 308 ACEISIVLEGEGMVRVV-NQQSLSSCKNDRKSESFMVEEGD--------------VFVVP 367

Query: 424 AGHPFSVFASPNHNLEIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVER 474
             HP +  +  N +   + F  +A  N    L G+ +++  ++R    + FN     ++ 
Sbjct: 368 KFHPMAQMSFENSSFVFMGFSTSAKTNHPQFLVGQSSVLKVLDRDVVAVSFNLSNETIKG 415

BLAST of Lsi02G022710 vs. TAIR 10
Match: AT4G36700.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 7.3e-20
Identity = 92/428 (21.50%), Postives = 179/428 (41.82%), Query Frame = 0

Query: 74  ESEEEEEEEEEE------KNPYVFEDEHFEGKFETGEGRIRVLQKFTQRSELLRGIENFR 133
           +SEE EE +          +P + + + ++  FET  G+I  +    Q      G+  ++
Sbjct: 26  KSEESEEYDVAVPSCCGFSSPLLIKKDQWKPIFETKFGQISTV----QIGNGCGGMGPYK 85

Query: 134 VSIVEANPFTFVIPTHFDAEIILFVARGRGTITVIKEKRASFDVKCGDVFRVPSGAPFYF 193
           +  +   P T ++P    ++++ FV  G G +  + E+  S +++ GDV+R+  G+ FY 
Sbjct: 86  IHSITLEPNTILLPLLLHSDMVFFVDSGSGILNWVDEEAKSTEIRLGDVYRLRPGSVFYL 145

Query: 194 INKDEH----QKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWD--LLEAAFKFP 253
            +K        KLK+  +        H     P  G  S      F +D  +L++AF  P
Sbjct: 146 QSKPVDIFLGTKLKLYAIFSNNDECLH----DPCFGAYSSITDLMFGFDETILQSAFGVP 205

Query: 254 RDKLERFFKQQKAGTIIKASREQIRSLSQHEEIIPKIWPFSEGETE-------------- 313
              +E    + K   I+  +      ++   ++ P++     G  +              
Sbjct: 206 EGIIELMRNRTKPPLIVSETL-CTPGVANTWQLQPRLLKLFAGSADLVDNKKKKEKKEKK 265

Query: 314 ------RPFNLLKQHPWQSNKFGRLFEAYPDEFSQLRDLGVAIAFANLTKGSMMAPHYNS 373
                 + FN+ +  P   + +GR       +   L+   V ++  NLT+GSMM PH+N 
Sbjct: 266 EKVKKAKTFNVFESEPDFESPYGRTITINRKDLKVLKGSMVGVSMVNLTQGSMMGPHWNP 325

Query: 374 RAMKIAVVVDGEGGFQMACPHLSSSSGRSGRWSEPEQERKGERTYQKIRGRLRRGVVFIV 433
            A +I++V+ G G  ++    +SS++                   + +R ++  G +F V
Sbjct: 326 WACEISIVLKGAGMVRVLRSSISSNTSSE---------------CKNVRFKVEEGDIFAV 385

Query: 434 PAGHPFSVFASPNHNLEIVCFEVNAYGNTKYLLAGKENIVNKMERVARELGFNTPGREVE 470
           P  HP +  +  N +L  V F  +A  N    LAG+++ +  ++R       N     ++
Sbjct: 386 PRLHPMAQMSFNNDSLVFVGFTTSAKNNEPQFLAGEDSALRMLDRQVLAASLNVSSVTID 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A2I4E5L62.1e-15758.67Vicilin Jug r 6.0101 OS=Juglans regia OX=51240 GN=LOC108986502 PE=1 SV=1[more]
Q8S4P94.9e-15459.44Vicilin Cor a 11.0101 OS=Corylus avellana OX=13451 PE=1 SV=1[more]
B4X6403.4e-13147.91Vicilin Pis v 3.0101 OS=Pistacia vera OX=55513 PE=1 SV=2[more]
B3STU42.2e-10143.99Vicilin Car i 2.0101 OS=Carya illinoinensis OX=32201 GN=pec2a1a PE=1 SV=1[more]
Q046723.1e-10041.17Sucrose-binding protein OS=Glycine max OX=3847 GN=SBP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BS821.5e-24387.73sucrose-binding protein-like OS=Cucumis melo OX=3656 GN=LOC103492635 PE=3 SV=1[more]
A0A0A0KAE24.2e-24187.40Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G380130 PE=4 SV=1[more]
A0A6J1JS851.5e-22783.74sucrose-binding protein-like OS=Cucurbita maxima OX=3661 GN=LOC111488449 PE=4 SV... [more]
A0A6J1GPW21.5e-22783.95sucrose-binding protein-like OS=Cucurbita moschata OX=3662 GN=LOC111456032 PE=4 ... [more]
A0A6J1D9211.5e-17767.42sucrose-binding protein-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_038898840.15.6e-25692.39vicilin Jug r 6.0101-like [Benincasa hispida][more]
XP_008451293.13.2e-24387.73PREDICTED: sucrose-binding protein-like [Cucumis melo][more]
XP_011659275.18.7e-24187.40sucrose-binding protein [Cucumis sativus] >KAE8646304.1 hypothetical protein Csa... [more]
KAG6575924.12.4e-22783.95Sucrose-binding protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG701... [more]
XP_022953499.13.2e-22783.95sucrose-binding protein-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G22640.12.1e-5932.87cupin family protein [more]
AT2G28490.15.2e-2623.00RmlC-like cupins superfamily protein [more]
AT2G18540.11.0e-2122.91RmlC-like cupins superfamily protein [more]
AT4G36700.17.3e-2021.50RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 63..86
NoneNo IPR availableGENE3D6.10.250.1700coord: 25..65
e-value: 2.7E-5
score: 25.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 361..383
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 66..88
NoneNo IPR availablePANTHERPTHR31189:SF1363 KDA GLOBULIN-LIKE PROTEINcoord: 7..483
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 7..483
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..22
score: 5.0
NoneNo IPR availableCDDcd02245cupin_7S_vicilin-like_Ccoord: 297..470
e-value: 1.60622E-79
score: 243.193
NoneNo IPR availableCDDcd02244cupin_7S_vicilin-like_Ncoord: 100..273
e-value: 9.15453E-85
score: 257.052
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 290..461
e-value: 2.5E-51
score: 186.5
coord: 96..246
e-value: 6.3E-5
score: 24.6
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 126..199
e-value: 3.8E-7
score: 29.8
coord: 290..454
e-value: 2.8E-30
score: 104.9
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 281..478
e-value: 2.7E-65
score: 221.4
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 77..269
e-value: 4.5E-77
score: 259.8
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 87..273
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 287..482

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G022710.1Lsi02G022710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034214 protein hexamerization
biological_process GO:0070207 protein homotrimerization
biological_process GO:0010431 seed maturation
cellular_component GO:0043245 extraorganismal space
molecular_function GO:0005507 copper ion binding
molecular_function GO:0045735 nutrient reservoir activity