Lsi02G022440 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G022440
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionSingle-stranded DNA-binding protein
Locationchr02: 28920568 .. 28926936 (+)
RNA-Seq ExpressionLsi02G022440
SyntenyLsi02G022440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTTTAGTATTTGATTTAATAAAAATCTAAAACCTTTTCATCAAACCCTCGCCCAACAGTTTGGCGCGCACAGAAGCACAGTCCAAGATAAGAGCGATGATAATGGCGCTGGAGCAAACCCTAGCTCTTTCGTCATCATTGTTATCAAAGAACTCCATCTTCTCCACCCCTAGAAACAACTTACGAATCGCTTCTTTCTCCCCTTTTCTCTCGCATCTAAACGTTCCTAAACAACTCCGTTTCGCAACTCGTTTCCCAAAATGCTCGGCTATGGAAAACTACAGCAATTACTCTGTAACCTACCCCAAACCTTCAGAAATTCCATGGAAGAAGGAGCTCAGTAACTCGGTCAGTCTCATCGGGACCGTTGGTATGCCTGTGGAGCTCAAGCACCTAGCATCGGGCAAGGATGTGGCTTGGACGCGACTTGCTGTTTGGAAGTCCTCTACTGAAACATCTTGGTACTAATCTATTGTTTCATTTCGAAATGGGCTTTAGTATGGTTGCATTTCGATCGAAGATTGAATGATAATGTGATTTTTTTTTATTTCAAGAATAAGTTCTTGAGTAGTTGGAATCGAGATGTTAAGAATATGGAAATTTTAGGACGATGGTTATAGAGTATATTTCTGTTTACTTCGAGTGCCATCCCTAAATTGGTGTCCCAATTTCATTAGGATGTGGAAACCATATGAATCTTTAAATGAACTTTTGTTTTGTATAAACGACGGCTTTCCGACCAAGTTTGGAAGGAAGTTACTGGAATGACTCTGTTTGGGCGTTGAGAGGGAGGATGTGTGGAATTTTGACACTTTTAAAAATCCTTTCTCGGGTCTTTCTTGTCTGTATAGCTTTTTCTCTGTACTTTCCATCTTACAAAAGCTGGTTATTAAAAATAAAAGAGTATGTTAATTTATAATAAAAGATTTAATTTTTGTTATAGGAGTTAGTATTTCATTTCTCTAAATCAATCCTACCACGAAGCCAACTAATTAATTGGCATTTTTTAGACACATTTTTTACCATGTGGATGCATGTCTAGAGCGTGTTCAGACGTATCCACGTCCGACATGGGCACTTTGTCTAAAACGAAGCTCCATGCTTCTTGGAGTGTCATCCTTGGAAATTTATGCTACTTCATTTCCAGATAAATTTGCAATTTTGCAAATCAAACGACCAATGCTGGCATTGGTCCATAGAATCTTACGATTCTTACCTTCTCTCTTGAAAGGGTGACCGCAAAAAATTTGAATGAGGAACTATTGCATGCCTGGGAGGATGATTAGGTTAAACCAAGTATGCAGAGGGTCAAATTTTTAAGCTGAATGGATAATGTATGGTAAGATTCTTTAAGTTTTTAGCACTAACCTTGCTGCACACGACGCACCAAAAGGGATAAAGCTAAGTGAATGTCATTCATATTGATACCTCCCAAAGCTAGTATCCAAAGAAAGAACTTGATGTTTTCTTGGACTCTTCTGTCTTCATACGACCTTCAAAAAGCTTTGATCAATGAGAAAGGCTGCCCTCCACCAATTTACCAAAAATGCAACTCTTAGAGTCTAAGGTATAGTTTACCTTAAATAATCAAGCACTTAAATTCTTCCATTCTCTAGAATACTCCAAAATCTCAATAAGGAGCAAACTACTCAATCTCGCCATTGTTCTAATTTCCTTCTAAAGTTGAGATTTCACCCTCCTCTGTCGTTGTTCTTAGAATCTTTGACTCATGTCCAGTTGAATTAGCAAGGGGTTGAAGTTTAAGAAACAGTGAATGGGTAGGAATTATGGATGATGGGACCAAGGATCTTCTAAAAATCTAACCAAAGAATCCTGATCAACTTTAAATGTCAAGAAGTGATCCACGTTTAGGTCCAGAAATAAAACCCTGTGCATATTTTCACTAATGGACACATCATCTGTCTTGTAGCTTCCTTACATATTTGCTCGTTGGCAAAGCTCCTCACTTCTCAGTGAGGGCACCTCCCTTCAAGAGAGCTAAGTGCCTGAGCTCATGCACAGGTACAACTTTTAAAATTATGACCTTGCACTGCCTCAAGTTAGCTCGCCAAAGAGATGTTCGGGAGACGATCGAGGAGTTCCTTCTCCATCCACCTTTCAAGGAGAAAGGCCAGTTTTTATGGTTAGTTTGGGTGTGCTATTTTGTGGGATATTTGGGGGAGAGGAATAACAGAGTGTTTAGAGGTGTGGAGAAGGACCATGGTGATGTTTGGTCCTTGGTGAGGTTCCATGTTTCTCTGTGGGCTTCGGTTTCAAAGACTTTTTGTAATTATCCTCTAGGTATTATCTTACTTTGTTGAAAATCCTTTTTTTAAAGGGGTTTTTTGCTTCTCTTGTATTATTTCATTCTTTTTCTCAATGAAAGCAGCTGTTTCAATTAAAAAGAAAAAAAAAAAAGGACCTTGCACTTTACGCTCAGAGGCTATTTTGCTTCAATACACTTTCGAGAAGATACACAAGTCTTTTTGGTGATCAAACTTACTACTTCAAGTCTGCATCATCATGTAAGTACTATTAGGACAAATGTTAAATACATTGTTAAAAAATTTATAGTCCTTAGCTAGGGAGATTATGAGAGAAGTAGTTATAAATAAAGTGAACCAGAGGGGTTGATGGGTGTAGAATTGCGTATGATCTTAGGAGAGAATTAGGCCTCTTTAATGCTTGGCAACCCATTGTAGTTTTCCTCCATTTATTAGCCATATCAATATCATATTTTCATTAATTTCCATTTTCAATTTTGTTTCCTATCAAATTGGTGTTGGAGCACCAACCACTGGGACTGTGTCCAACTATTTTCTAGTTAGCTCCTAGTTTCCTTACTAATGGGTTAGTGCTGCTGATTTTTTAGGGAACAGAATATTAGTATCACCTAAAGAGCCCCCCACCTTTGGTGGCTTCCTTTCCACTTCACCCACATATTTTCCAAACAGCATTATTTAGCTGGAGTCCCTTTTTGTAGAGGGAGGTCACCTTTTTGTGGGGTTGTTTTTTGTTTTATGCCCTTTATATATTTCCCCCCAAAGAAAGTTGTTTACACATATATATATCACCTAGCAGGCCAGAAAAATGTTTGGTTCCTGAAGCATAATCGCCCCAGGCCTCTTCTGCAGTAAAACATCCTTGATGAGCCTCCCTCTGAACCCCTAAAATTCTAAAAGGAACCTTCACTAAGACCCTAGGGGACTCAGTGTAGCGCCACCCATCTTAGCCAAAAAAAATTTCAAAGTTAGGCCACTCAAGAGACAATTCTTTAAGTGGTGGCCTAGTGGGCTAAGTCTTTTGAACACTTAGTTACCCATTGTTACTTTGCTCTGTTGATTAGCATTTCCAATAGCTTGTTTTGTTAGCTTCCCTTTCAATTTTTGGTTCCTATCACACTTTTGTTTTCTTTGACGATGAGAAACATGTTAAATTGAGATAGATAAGTAAAGAGGCGGGGAAGAAGGCACGACTTTGTTTAAAGACTCCTCATTTCTTTCAATCCAAATCTCCCACAAAGTGCTACATTCATCCCTTTGCACTGCAGAACTTTAAAACTCTGGTTTCTAGAGAGATACAGCATGAAAGAGCAAATAAAATGACAATTAGGACTATGATCCAAATCAGGATTTTGCTCTTTCAGGATAAATTTAACATTCTGGGATGAATTGGCACATGTCGCTTCTCAACACGTCCAGAAAGGCAACCAGATATATGTATCGGGTCGGCTGGTAGCAGATTTAGTTGAAGCTGATGATGGGAAACAGCAAACATACTATAAGGTTTTGCCGTCCTTGTTCACATTGTTCCATGGTTTTTTGTTCGTTCGTAAGGTAGATGCTGGCGCTTTCATATCTTTTGCAGGTGGTAGTTCAGCAACTGAATTTTATAGAAAGAAGCAACTCAACAGGACCCCATTATGACCAAGACTCTAATTCCGTAATGGCAGGCATGGGATATTTTTTTAATACAAGATTATTGGTTGACACTTTATTTTGCTTCAAAATCTTATTATTTGGTGTCCTATCACATTTCTTCGCAGGGAGAAAGCTCGGCAGTGGTGCTGTTGATAATAATATGGGGTCCACACAAGAACTATGGCAAGCATTCTTTGCTAATCCCGTTGACTGGTGGGACAATAGGAAGAATAAGGTGCTTGATTAATTCTCTTGCTATCTTGTTTTTGGATTCAAATGTTCTTGACATGGACTAAGTTTAGTATTTGCTTGTGTTTTTATGCCTGTGATTACTGATATTCGTTTTTTTCAAATTGATGCAAGAAAAAACCTTAGGAACTTACAACGAACCGCATAGATAGCATACATTTTACATTTTTTTAACTAATCTTAATTAAACTTTGCCAAAATGAGCATAGCTGAACTGAGTTGGAGTTTCGATTCCCGCACCCCACATACTGTCAAAAGAGGAAGAAAAAAAACTAATTTTTGAGGGATATGAACTCCCAAGGGAAGTTAGAAGAAAGAAAGAAAAAAAAACAAAATCAAGAACTTTGAGACAAATACAAGGGAATAATAGAGGTCAGCTCACATAACAATGAATCTGTTAAAGAACAATAGATTTATTCAATAAATTATGCCTTTTTTTATATATTTGTGAGTGTTCGGGCCAGCTTATGCGCACCTCGACTAATCTCACGGGACAACCCGCCTGACCCTACAATATTTGGGTGCCAAGGAAACCCGTAGGAAAATTACACCTTCTTTGAACCAAAAACTTTTAGAGCTTGCAAATCATGGTCGTGGTGAAGAAGGAAGACTCTACAAACCACTCCAAAATCAGCTGCAGCAACCACTGCCAAAAAAGAAATTTCATCCAGAGCTTGCTGAAAAAACGTTGGATGGATATTTAGGAGCTTTACAGCAACAAATCTTCTAATTCCAGAAACTTAGAGCAACCACACAACTTTACAAGACGAAAAGCTCAAAAATCTATGCTAACAAAATTGAGGAGAGTAAGATTTTTTAATAGCAAAGGAACTGAGAGCAACAACAGCCACAAGACTAACAAATGGGAGATTTAAAACAACCTGAGAGAGAGCAACTGAGGCTATAAAGATTTGCAAAAGGCCCTAAACAAAATCAATGAAGCTGAAAAAGAAACTTATCAATCGCTGAGAAAGGCAGAACAGCAATAAAGAAGGAAGGAGTCATCACTAGGCATAGAAAAAAAAAGCCTCCACGAGACAAACTGACTCAAACGCACAGCCACAAAGACAAGATGAAATCTCTATGAGCCCATATGCTTGAAAACTTGAACTTCTGAATGTCTTATGATTTTCTCCTGTGAACCTCAAGACCACACACCTCTAGGAAGGGGGAAAAAGTATCTTTATCTTCTAATGTGTTCTTGTGAATGAAGGAAAGGAGGGACGTTATATCAGACAAATAAGGAGAGCTAATTTCTTGAAAAAAGTTACATAGAACCCTATCAATAGCTATTAGCTTGTTCAGATGAAATTTTATCATTTCCATTGTCAATTGGAAAATTAAAATCCAATTCCTCACTATTAAGACTTGCCATGAATTCGTAGTCACACCCTTTGACAGACATTTATGAGTACTTAAACTTTAATTGTTTAAAGAAGTATAGAAGCTAAACCAAGTACGAGCAACACAGGAAAGTTGCCAACAGGCTTTTTTAGTTATCATATCTAAATATTATTATTTATTGTATGTATGAAATGTCAGGCCTCAATGATTTAAAAGGAAACAAGGAATTAGTAGCCTCTTGCAGGCCATTTTAGACATTCCTATAGTTACCTTTTGTTAGTATTTTTAGGTTTCCTTCACTTGAATCCTTGATGAGAGATGAGTTTCACTCTTTAATGTTAATACATTGGCATTTTGATTCTGCTTGCAGAAGAACCCAAAATATCCAGATTTTAAACACAAGGACACTGGGGAAGCCTTGTGGGTTGAGGGCAGGTATAATCCACCCTGGGTGAAGTCCCAGCTTGCATTATTGGACATGAGAATGAGTTCTCTTGATGATCAGGAAGCCAAAATGACCAACTTTGTAACTAATTTTGATTTCCCATCTTATTAAACTGTCTCTGGATTCCTTTAAATTGTTTCCAACAGGGGTATTCACCAAATATATTTGGAAAGATTCTTTTGTGGTGTCTGGCTTTGTATAGTTTGTAAATTGATGTACTGTATATTAGATTGTTAGTAATTTAAATATTTCTTCAGGCTCCAAGTTCAGTGCCTGTTAAGGAAGGGAAAAAAAGAAAAGAAAAGAAAAGTAAAGAAAAGGCATGGAATGTTTTGATATTTAGTTGGCAGTGATTCAAATTAAGGTCAACTCATTTTTGCCTTTCAGTTGATAATAGAAAGTATAGTGA

mRNA sequence

CTTTTTTAGTATTTGATTTAATAAAAATCTAAAACCTTTTCATCAAACCCTCGCCCAACAGTTTGGCGCGCACAGAAGCACAGTCCAAGATAAGAGCGATGATAATGGCGCTGGAGCAAACCCTAGCTCTTTCGTCATCATTGTTATCAAAGAACTCCATCTTCTCCACCCCTAGAAACAACTTACGAATCGCTTCTTTCTCCCCTTTTCTCTCGCATCTAAACGTTCCTAAACAACTCCGTTTCGCAACTCGTTTCCCAAAATGCTCGGCTATGGAAAACTACAGCAATTACTCTGTAACCTACCCCAAACCTTCAGAAATTCCATGGAAGAAGGAGCTCAGTAACTCGGTCAGTCTCATCGGGACCGTTGGTATGCCTGTGGAGCTCAAGCACCTAGCATCGGGCAAGGATGTGGCTTGGACGCGACTTGCTGTTTGGAAGTCCTCTACTGAAACATCTTGGATAAATTTAACATTCTGGGATGAATTGGCACATGTCGCTTCTCAACACGTCCAGAAAGGCAACCAGATATATGTATCGGGTCGGCTGGTAGCAGATTTAGTTGAAGCTGATGATGGGAAACAGCAAACATACTATAAGGTTTTGCCGTCCTTGTTCACATTGTTCCATGGTTTTTTGTTCGTTCGTAAGGTAGATGCTGGCGCTTTCATATCTTTTGCAGGTGGGAGAAAGCTCGGCAGTGGTGCTGTTGATAATAATATGGGGTCCACACAAGAACTATGGCAAGCATTCTTTGCTAATCCCGTTGACTGGTGGGACAATAGGAAGAATAAGAAGAACCCAAAATATCCAGATTTTAAACACAAGGACACTGGGGAAGCCTTGTGGGTTGAGGGCAGGTATAATCCACCCTGGGTGAAGTCCCAGCTTGCATTATTGGACATGAGAATGAGTTCTCTTGATGATCAGGAAGCCAAAATGACCAACTTTGTAACTAATTTTGATTTCCCATCTTATTAAACTGTCTCTGGATTCCTTTAAATTGTTTCCAACAGGGGTATTCACCAAATATATTTGGAAAGATTCTTTTGTGGTGTCTGGCTTTGTATAGTTTGTAAATTGATGTACTGTATATTAGATTGTTAGTAATTTAAATATTTCTTCAGGCTCCAAGTTCAGTGCCTGTTAAGGAAGGGAAAAAAAGAAAAGAAAAGAAAAGTAAAGAAAAGGCATGGAATGTTTTGATATTTAGTTGGCAGTGATTCAAATTAAGGTCAACTCATTTTTGCCTTTCAGTTGATAATAGAAAGTATAGTGA

Coding sequence (CDS)

ATGATAATGGCGCTGGAGCAAACCCTAGCTCTTTCGTCATCATTGTTATCAAAGAACTCCATCTTCTCCACCCCTAGAAACAACTTACGAATCGCTTCTTTCTCCCCTTTTCTCTCGCATCTAAACGTTCCTAAACAACTCCGTTTCGCAACTCGTTTCCCAAAATGCTCGGCTATGGAAAACTACAGCAATTACTCTGTAACCTACCCCAAACCTTCAGAAATTCCATGGAAGAAGGAGCTCAGTAACTCGGTCAGTCTCATCGGGACCGTTGGTATGCCTGTGGAGCTCAAGCACCTAGCATCGGGCAAGGATGTGGCTTGGACGCGACTTGCTGTTTGGAAGTCCTCTACTGAAACATCTTGGATAAATTTAACATTCTGGGATGAATTGGCACATGTCGCTTCTCAACACGTCCAGAAAGGCAACCAGATATATGTATCGGGTCGGCTGGTAGCAGATTTAGTTGAAGCTGATGATGGGAAACAGCAAACATACTATAAGGTTTTGCCGTCCTTGTTCACATTGTTCCATGGTTTTTTGTTCGTTCGTAAGGTAGATGCTGGCGCTTTCATATCTTTTGCAGGTGGGAGAAAGCTCGGCAGTGGTGCTGTTGATAATAATATGGGGTCCACACAAGAACTATGGCAAGCATTCTTTGCTAATCCCGTTGACTGGTGGGACAATAGGAAGAATAAGAAGAACCCAAAATATCCAGATTTTAAACACAAGGACACTGGGGAAGCCTTGTGGGTTGAGGGCAGGTATAATCCACCCTGGGTGAAGTCCCAGCTTGCATTATTGGACATGAGAATGAGTTCTCTTGATGATCAGGAAGCCAAAATGACCAACTTTGTAACTAATTTTGATTTCCCATCTTATTAA

Protein sequence

MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAMENYSNYSVTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTETSWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGFLFVRKVDAGAFISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPSY
Homology
BLAST of Lsi02G022440 vs. ExPASy Swiss-Prot
Match: Q9SX99 (Protein OSB1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB1 PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.2e-23
Identity = 72/226 (31.86%), Postives = 109/226 (48.23%), Query Frame = 0

Query: 69  YPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWK--SSTETSWINLT 128
           + KP     K  L NSVSL+G V   +++ +    +   +T L V    +   +  I+L 
Sbjct: 42  FKKPLSTKLKFNLVNSVSLMGFVDRSIQVMNTGPDRFGVFTILRVKDPLNPNRSFRISLR 101

Query: 129 FWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGFLFVRKV 188
            WD +A     H++  + I VSGRL            ++Y K    +++  +   +  KV
Sbjct: 102 MWDAMARTCIAHLKLNDHILVSGRL------------ESYSKSSSDVYSGLN-LDYQVKV 161

Query: 189 DAGAFISFAGGRKLGSGAVDNNMGSTQE-----------LWQAFFANPVDWWDNRKNKKN 248
               +++      L S    N    T++           LWQ FF+NP DWWDNR+NKKN
Sbjct: 162 AEVNYVAAPPSHVLDSQISKNPKTKTEDDIEESKKDEIYLWQVFFSNPYDWWDNRRNKKN 221

Query: 249 PKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAK 282
           PK PDFKHKDTGEALW+     P W+  +L L D +    D+++ +
Sbjct: 222 PKQPDFKHKDTGEALWLCSDL-PDWITRRLELFDQKNRFYDEEKTR 253

BLAST of Lsi02G022440 vs. ExPASy Swiss-Prot
Match: Q8GXH3 (Protein OSB2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB2 PE=1 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 1.3e-19
Identity = 65/213 (30.52%), Postives = 105/213 (49.30%), Query Frame = 0

Query: 70  PKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTETS-WINLTFW 129
           P+P+EI ++ E++N V+LIG V  PV+ +  + GK  A T ++   +S  +  WI + F 
Sbjct: 86  PRPNEIAYESEVANWVNLIGFVDQPVQFEASSDGKFWAGTVISQRSASDSSGFWIPIIFE 145

Query: 130 DELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGFLFVRKVDA 189
            +LA  A+++V K +QI+VSG+L  D          TY +    +      F+       
Sbjct: 146 GDLAKTAARYVSKDDQIHVSGKLFID----SPPPNMTYAQANVQVLVQNLNFIQPMSPSP 205

Query: 190 GAFISFAGGRKLGSGAVDNNMGSTQEL--------WQAFFANPVDWWDNRKNKKN----P 249
             F+  +   K  SG       + Q++        W     NP +WWD+R+NK N    P
Sbjct: 206 SPFMVMSSSEKEESGIKKQPARAKQDIVIDEASDSWNHLIENPKEWWDHRENKVNGLVKP 265

Query: 250 KYPDFKHKDTGEALWVEGRYNPPWVKSQLALLD 270
           ++PDFK KD+  +LW+     P WV  +L  L+
Sbjct: 266 RHPDFKSKDSSFSLWLNKA--PNWVLPKLEGLE 292

BLAST of Lsi02G022440 vs. ExPASy Swiss-Prot
Match: Q9FYJ2 (Protein OSB4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB4 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 9.0e-18
Identity = 60/212 (28.30%), Postives = 105/212 (49.53%), Query Frame = 0

Query: 66  SVTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTETS---- 125
           S  +P+P E+P++ +++NS+ LIG V  PV+      GK  A T ++   SS   S    
Sbjct: 56  SEEWPRPMEVPYQPKIANSIDLIGYVHQPVQFDSTLDGKFWAGTVISHEPSSDSKSESDS 115

Query: 126 ----WINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLF 185
               WI + F  +LAH A+ +++K ++++++G+++ D++++     Q + ++  S    F
Sbjct: 116 SSNFWIPVLFEGDLAHTANSYLKKNDRVHITGQILGDVIQSGANSDQAHVQLFKSFHGSF 175

Query: 186 HGFLFVRKVDAGAFISFAGGRKLGSGAVDNNMG------STQ-------ELWQAFFANPV 245
              + VR +    +I  +         +D N G      S Q        LW      P 
Sbjct: 176 SHQVMVRDLH---YIEGSKAMPKVLPTLDQNEGVLKHSASVQRGREFGTNLWFDLVDKPN 235

Query: 246 DWWDNRKNKK----NPKYPDFKHKDTGEALWV 253
           +W D R+ K+    NPK+PDFK KD  +ALW+
Sbjct: 236 EWCDYREMKQNGSVNPKHPDFKKKDGSQALWL 264

BLAST of Lsi02G022440 vs. ExPASy Swiss-Prot
Match: Q8GWJ4 (Protein OSB3, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB3 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.1e-11
Identity = 29/53 (54.72%), Postives = 38/53 (71.70%), Query Frame = 0

Query: 213 QELWQAFFANPVDWWDNRKNKKNPKYPDFKHKDTGEALWVEGRYNPPWVKSQL 266
           +E+W+    NP  WWDNR +K+NPK PDFKHK+TGEALW+    +P W  S+L
Sbjct: 377 EEIWKNLVENPSKWWDNRLDKRNPKGPDFKHKETGEALWIGD--SPTWALSKL 427

BLAST of Lsi02G022440 vs. ExPASy Swiss-Prot
Match: P0AGE2 (Single-stranded DNA-binding protein OS=Escherichia coli O157:H7 OX=83334 GN=ssb PE=3 SV=2)

HSP 1 Score: 55.1 bits (131), Expect = 1.6e-06
Identity = 35/140 (25.00%), Postives = 63/140 (45.00%), Query Frame = 0

Query: 83  NSVSLIGTVGMPVELKHLASGKDVA---------WTRLAVWKSSTETSWINLTFWDELAH 142
           N V L+G +G   E++++ +G  VA         W   A  +   +T W  +  + +LA 
Sbjct: 7   NKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKLAE 66

Query: 143 VASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGFLFVRKVDAGAFIS 202
           VAS++++KG+Q+Y+ G+L         G+ +   +V+               V+ G  + 
Sbjct: 67  VASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVV---------------VNVGGTMQ 126

Query: 203 FAGGRKLGSGAVDNNMGSTQ 214
             GGR+ G      N+G  Q
Sbjct: 127 MLGGRQGGGAPAGGNIGGGQ 131

BLAST of Lsi02G022440 vs. ExPASy TrEMBL
Match: A0A1S3BRV3 (protein OSB2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492617 PE=4 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.3e-132
Identity = 244/301 (81.06%), Postives = 258/301 (85.71%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           M +ALEQTL LSSS  SKNSIFS PRN LRI+SF PF S+LN PK LRFATRFPKCSAME
Sbjct: 3   MALALEQTLTLSSSFSSKNSIFSIPRNQLRISSFFPFHSYLNPPKHLRFATRFPKCSAME 62

Query: 61  NYSNYS-VTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTE 120
           NY+NYS VTYPKPSEIPWKKELSNSV+LIGTVG+PVELKHL SGKDVAWTRLAVWKSSTE
Sbjct: 63  NYTNYSAVTYPKPSEIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 122

Query: 121 TSWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHG 180
           TSWI+LTFWDELAH+ASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKV+         
Sbjct: 123 TSWISLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLN---- 182

Query: 181 FLFVRKVDAGA------FISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNK 240
             F+ + ++ A        S   GRK  SGAVDN   STQELWQAFFANPVDWWDNRKNK
Sbjct: 183 --FIERSNSTAPHYDQDSNSIMAGRKPSSGAVDNT-ASTQELWQAFFANPVDWWDNRKNK 242

Query: 241 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPS 295
           KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQE KMTNFV+NFDFPS
Sbjct: 243 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEVKMTNFVSNFDFPS 296

BLAST of Lsi02G022440 vs. ExPASy TrEMBL
Match: A0A5D3DR47 (Protein OSB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00530 PE=4 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.6e-131
Identity = 243/301 (80.73%), Postives = 258/301 (85.71%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           M +ALEQTL LSSS  SKNSIFS PRN LRI+SF PF S+LN PK LRFATRFPKCSAME
Sbjct: 3   MALALEQTLTLSSSFSSKNSIFSIPRNQLRISSFFPFHSYLNPPKHLRFATRFPKCSAME 62

Query: 61  NYSNYS-VTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTE 120
           NY+NYS VTYPKPSEIPWKKELSNSV+LIGTVG+PVELKHL SGKDVAWTRLAVWKSSTE
Sbjct: 63  NYTNYSAVTYPKPSEIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 122

Query: 121 TSWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHG 180
           TSWI+LTFWDELAH+ASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKV+         
Sbjct: 123 TSWISLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLN---- 182

Query: 181 FLFVRKVDAGA------FISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNK 240
             F+ + ++ A        S   GRK  SGAVDN   STQELWQAFFANPVDWWDNRKNK
Sbjct: 183 --FIERSNSTAPHYDQDSNSIMAGRKPSSGAVDNT-ASTQELWQAFFANPVDWWDNRKNK 242

Query: 241 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPS 295
           K+PKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQE KMTNFV+NFDFPS
Sbjct: 243 KSPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEVKMTNFVSNFDFPS 296

BLAST of Lsi02G022440 vs. ExPASy TrEMBL
Match: A0A0A0K4W2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G378540 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.1e-130
Identity = 242/301 (80.40%), Postives = 256/301 (85.05%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           M+MALEQTL LSSSL SKNSIFS PRN L I+SF PF S+LN PK LRF   FPKCSAME
Sbjct: 1   MVMALEQTLILSSSLSSKNSIFSIPRNQLPISSFFPFHSYLNPPKHLRFPAPFPKCSAME 60

Query: 61  NYSNYS-VTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTE 120
           NY+NYS VTYPKPS+IPWKKELSNSV+LIGTVG+PVELKHL SGKDVAWTRLAVWKSSTE
Sbjct: 61  NYTNYSAVTYPKPSQIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 120

Query: 121 TSWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHG 180
           TSWI LTFWDELAH+ASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKV+         
Sbjct: 121 TSWIGLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLN---- 180

Query: 181 FLFVRKVDAGA------FISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNK 240
             F+ + ++ A        S   GRK GSGAVDN  GSTQELWQAFFANPVDWWDNRKNK
Sbjct: 181 --FIERSNSTAPHYDQDSNSIMAGRKPGSGAVDNT-GSTQELWQAFFANPVDWWDNRKNK 240

Query: 241 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPS 295
           KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRM SLDDQEAKM NFVT+FDFPS
Sbjct: 241 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMGSLDDQEAKMANFVTSFDFPS 294

BLAST of Lsi02G022440 vs. ExPASy TrEMBL
Match: A0A6J1DQC6 (protein OSB2, chloroplastic-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022838 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.1e-126
Identity = 230/294 (78.23%), Postives = 247/294 (84.01%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           M+MALEQTL+LSSSLL K+++ S PRN  RIAS    LSHL+  K+LRFA RFPKCSAME
Sbjct: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIAS----LSHLDAAKRLRFARRFPKCSAME 60

Query: 61  NYSNYSVTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTET 120
           N+SNYS +Y KP EIPW KELSNSVSLIGTVG PVE+KHLASGKDVAWTRLAVWKSSTET
Sbjct: 61  NFSNYSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTET 120

Query: 121 SWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGF 180
           SWINLTFWDELAH+A QHVQKGNQI+VSGRLVADLVEADDGKQQTYYKV+          
Sbjct: 121 SWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERS 180

Query: 181 LFVRKVDAGAFISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPD 240
           +           S   GRKLG+GA D NMGSTQELWQAFFANPVDWWDNRKNKKNPKYPD
Sbjct: 181 ISTAPHHDQDSNSIVAGRKLGNGAAD-NMGSTQELWQAFFANPVDWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPSY 295
           FKHKDTGEALWVEGRYNPPWVKSQLA+LDMRM SLD+QEAKM NFVTN DFPSY
Sbjct: 241 FKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 289

BLAST of Lsi02G022440 vs. ExPASy TrEMBL
Match: A0A6J1DNW7 (protein OSB2, chloroplastic-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022838 PE=4 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.9e-124
Identity = 229/294 (77.89%), Postives = 246/294 (83.67%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           M+MALEQTL+LSSSLL K+++ S PRN  RIAS    LSHL+  K+LRFA RFPKCSAME
Sbjct: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIAS----LSHLDAAKRLRFARRFPKCSAME 60

Query: 61  NYSNYSVTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTET 120
           N+SNYS +Y KP EIPW KELSNSVSLIGTVG PVE+KHLASGKDVAWTRLAVWKSSTET
Sbjct: 61  NFSNYSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTET 120

Query: 121 SWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGF 180
           SWINLTFWDELAH+A QHVQKGNQI+VSGRLVADLVEADDGKQQTYYKV+          
Sbjct: 121 SWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERS 180

Query: 181 LFVRKVDAGAFISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPD 240
           +           S   GRKLG+GA D NMGSTQELWQAFFANPVDWWDNRKN KNPKYPD
Sbjct: 181 ISTAPHHDQDSNSIVAGRKLGNGAAD-NMGSTQELWQAFFANPVDWWDNRKN-KNPKYPD 240

Query: 241 FKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPSY 295
           FKHKDTGEALWVEGRYNPPWVKSQLA+LDMRM SLD+QEAKM NFVTN DFPSY
Sbjct: 241 FKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 288

BLAST of Lsi02G022440 vs. NCBI nr
Match: XP_038896738.1 (protein OSB2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 502.3 bits (1292), Expect = 2.9e-138
Identity = 253/299 (84.62%), Postives = 261/299 (87.29%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           MIMALEQTL LSSSLLSKNSIFS PRN LRI+SF PFLS LN PK LRFATRFPKCSAME
Sbjct: 1   MIMALEQTLTLSSSLLSKNSIFSIPRNELRISSFYPFLSGLNAPKHLRFATRFPKCSAME 60

Query: 61  NYSNYSVTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTET 120
           NYS+YS TYPKPSEIPWKK+LSNSVSLIGTVGMPVELKHLA+GKDVAWTRLAVWKSSTET
Sbjct: 61  NYSSYSPTYPKPSEIPWKKDLSNSVSLIGTVGMPVELKHLATGKDVAWTRLAVWKSSTET 120

Query: 121 SWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGF 180
           SWINLTFWDELAHVASQH+QKGNQIYVSGRLVADLVEADDGKQQTYYKV+          
Sbjct: 121 SWINLTFWDELAHVASQHIQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIE-- 180

Query: 181 LFVRKVDAGAFI-----SFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNKKN 240
              R    G        S   G+KLGSGAVD N+GSTQELWQAFFANPVDWWDNRKNKKN
Sbjct: 181 ---RSNSTGPHYDQDSNSVMAGKKLGSGAVD-NVGSTQELWQAFFANPVDWWDNRKNKKN 240

Query: 241 PKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPSY 295
           PKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKM NFVTNFDFPSY
Sbjct: 241 PKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMANFVTNFDFPSY 293

BLAST of Lsi02G022440 vs. NCBI nr
Match: XP_008451268.1 (PREDICTED: protein OSB2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 480.7 bits (1236), Expect = 8.9e-132
Identity = 244/301 (81.06%), Postives = 258/301 (85.71%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           M +ALEQTL LSSS  SKNSIFS PRN LRI+SF PF S+LN PK LRFATRFPKCSAME
Sbjct: 3   MALALEQTLTLSSSFSSKNSIFSIPRNQLRISSFFPFHSYLNPPKHLRFATRFPKCSAME 62

Query: 61  NYSNYS-VTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTE 120
           NY+NYS VTYPKPSEIPWKKELSNSV+LIGTVG+PVELKHL SGKDVAWTRLAVWKSSTE
Sbjct: 63  NYTNYSAVTYPKPSEIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 122

Query: 121 TSWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHG 180
           TSWI+LTFWDELAH+ASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKV+         
Sbjct: 123 TSWISLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLN---- 182

Query: 181 FLFVRKVDAGA------FISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNK 240
             F+ + ++ A        S   GRK  SGAVDN   STQELWQAFFANPVDWWDNRKNK
Sbjct: 183 --FIERSNSTAPHYDQDSNSIMAGRKPSSGAVDNT-ASTQELWQAFFANPVDWWDNRKNK 242

Query: 241 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPS 295
           KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQE KMTNFV+NFDFPS
Sbjct: 243 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEVKMTNFVSNFDFPS 296

BLAST of Lsi02G022440 vs. NCBI nr
Match: KAA0059696.1 (protein OSB2 [Cucumis melo var. makuwa] >TYK26133.1 protein OSB2 [Cucumis melo var. makuwa])

HSP 1 Score: 478.8 bits (1231), Expect = 3.4e-131
Identity = 243/301 (80.73%), Postives = 258/301 (85.71%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           M +ALEQTL LSSS  SKNSIFS PRN LRI+SF PF S+LN PK LRFATRFPKCSAME
Sbjct: 3   MALALEQTLTLSSSFSSKNSIFSIPRNQLRISSFFPFHSYLNPPKHLRFATRFPKCSAME 62

Query: 61  NYSNYS-VTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTE 120
           NY+NYS VTYPKPSEIPWKKELSNSV+LIGTVG+PVELKHL SGKDVAWTRLAVWKSSTE
Sbjct: 63  NYTNYSAVTYPKPSEIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 122

Query: 121 TSWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHG 180
           TSWI+LTFWDELAH+ASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKV+         
Sbjct: 123 TSWISLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLN---- 182

Query: 181 FLFVRKVDAGA------FISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNK 240
             F+ + ++ A        S   GRK  SGAVDN   STQELWQAFFANPVDWWDNRKNK
Sbjct: 183 --FIERSNSTAPHYDQDSNSIMAGRKPSSGAVDNT-ASTQELWQAFFANPVDWWDNRKNK 242

Query: 241 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPS 295
           K+PKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQE KMTNFV+NFDFPS
Sbjct: 243 KSPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEVKMTNFVSNFDFPS 296

BLAST of Lsi02G022440 vs. NCBI nr
Match: XP_004146958.1 (protein OSB2, chloroplastic isoform X1 [Cucumis sativus] >KGN44765.1 hypothetical protein Csa_016591 [Cucumis sativus])

HSP 1 Score: 476.1 bits (1224), Expect = 2.2e-130
Identity = 242/301 (80.40%), Postives = 256/301 (85.05%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           M+MALEQTL LSSSL SKNSIFS PRN L I+SF PF S+LN PK LRF   FPKCSAME
Sbjct: 1   MVMALEQTLILSSSLSSKNSIFSIPRNQLPISSFFPFHSYLNPPKHLRFPAPFPKCSAME 60

Query: 61  NYSNYS-VTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTE 120
           NY+NYS VTYPKPS+IPWKKELSNSV+LIGTVG+PVELKHL SGKDVAWTRLAVWKSSTE
Sbjct: 61  NYTNYSAVTYPKPSQIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 120

Query: 121 TSWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHG 180
           TSWI LTFWDELAH+ASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKV+         
Sbjct: 121 TSWIGLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLN---- 180

Query: 181 FLFVRKVDAGA------FISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNK 240
             F+ + ++ A        S   GRK GSGAVDN  GSTQELWQAFFANPVDWWDNRKNK
Sbjct: 181 --FIERSNSTAPHYDQDSNSIMAGRKPGSGAVDNT-GSTQELWQAFFANPVDWWDNRKNK 240

Query: 241 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPS 295
           KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRM SLDDQEAKM NFVT+FDFPS
Sbjct: 241 KNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMGSLDDQEAKMANFVTSFDFPS 294

BLAST of Lsi02G022440 vs. NCBI nr
Match: XP_022155802.1 (protein OSB2, chloroplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 461.8 bits (1187), Expect = 4.3e-126
Identity = 230/294 (78.23%), Postives = 247/294 (84.01%), Query Frame = 0

Query: 1   MIMALEQTLALSSSLLSKNSIFSTPRNNLRIASFSPFLSHLNVPKQLRFATRFPKCSAME 60
           M+MALEQTL+LSSSLL K+++ S PRN  RIAS    LSHL+  K+LRFA RFPKCSAME
Sbjct: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIAS----LSHLDAAKRLRFARRFPKCSAME 60

Query: 61  NYSNYSVTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTET 120
           N+SNYS +Y KP EIPW KELSNSVSLIGTVG PVE+KHLASGKDVAWTRLAVWKSSTET
Sbjct: 61  NFSNYSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTET 120

Query: 121 SWINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGF 180
           SWINLTFWDELAH+A QHVQKGNQI+VSGRLVADLVEADDGKQQTYYKV+          
Sbjct: 121 SWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERS 180

Query: 181 LFVRKVDAGAFISFAGGRKLGSGAVDNNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPD 240
           +           S   GRKLG+GA D NMGSTQELWQAFFANPVDWWDNRKNKKNPKYPD
Sbjct: 181 ISTAPHHDQDSNSIVAGRKLGNGAAD-NMGSTQELWQAFFANPVDWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMTNFVTNFDFPSY 295
           FKHKDTGEALWVEGRYNPPWVKSQLA+LDMRM SLD+QEAKM NFVTN DFPSY
Sbjct: 241 FKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 289

BLAST of Lsi02G022440 vs. TAIR 10
Match: AT1G47720.1 (Primosome PriB/single-strand DNA-binding )

HSP 1 Score: 110.5 bits (275), Expect = 2.3e-24
Identity = 72/226 (31.86%), Postives = 109/226 (48.23%), Query Frame = 0

Query: 69  YPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWK--SSTETSWINLT 128
           + KP     K  L NSVSL+G V   +++ +    +   +T L V    +   +  I+L 
Sbjct: 42  FKKPLSTKLKFNLVNSVSLMGFVDRSIQVMNTGPDRFGVFTILRVKDPLNPNRSFRISLR 101

Query: 129 FWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGFLFVRKV 188
            WD +A     H++  + I VSGRL            ++Y K    +++  +   +  KV
Sbjct: 102 MWDAMARTCIAHLKLNDHILVSGRL------------ESYSKSSSDVYSGLN-LDYQVKV 161

Query: 189 DAGAFISFAGGRKLGSGAVDNNMGSTQE-----------LWQAFFANPVDWWDNRKNKKN 248
               +++      L S    N    T++           LWQ FF+NP DWWDNR+NKKN
Sbjct: 162 AEVNYVAAPPSHVLDSQISKNPKTKTEDDIEESKKDEIYLWQVFFSNPYDWWDNRRNKKN 221

Query: 249 PKYPDFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAK 282
           PK PDFKHKDTGEALW+     P W+  +L L D +    D+++ +
Sbjct: 222 PKQPDFKHKDTGEALWLCSDL-PDWITRRLELFDQKNRFYDEEKTR 253

BLAST of Lsi02G022440 vs. TAIR 10
Match: AT4G20010.1 (plastid transcriptionally active 9 )

HSP 1 Score: 98.6 bits (244), Expect = 8.9e-21
Identity = 65/213 (30.52%), Postives = 105/213 (49.30%), Query Frame = 0

Query: 70  PKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTETS-WINLTFW 129
           P+P+EI ++ E++N V+LIG V  PV+ +  + GK  A T ++   +S  +  WI + F 
Sbjct: 86  PRPNEIAYESEVANWVNLIGFVDQPVQFEASSDGKFWAGTVISQRSASDSSGFWIPIIFE 145

Query: 130 DELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGFLFVRKVDA 189
            +LA  A+++V K +QI+VSG+L  D          TY +    +      F+       
Sbjct: 146 GDLAKTAARYVSKDDQIHVSGKLFID----SPPPNMTYAQANVQVLVQNLNFIQPMSPSP 205

Query: 190 GAFISFAGGRKLGSGAVDNNMGSTQEL--------WQAFFANPVDWWDNRKNKKN----P 249
             F+  +   K  SG       + Q++        W     NP +WWD+R+NK N    P
Sbjct: 206 SPFMVMSSSEKEESGIKKQPARAKQDIVIDEASDSWNHLIENPKEWWDHRENKVNGLVKP 265

Query: 250 KYPDFKHKDTGEALWVEGRYNPPWVKSQLALLD 270
           ++PDFK KD+  +LW+     P WV  +L  L+
Sbjct: 266 RHPDFKSKDSSFSLWLNKA--PNWVLPKLEGLE 292

BLAST of Lsi02G022440 vs. TAIR 10
Match: AT4G20010.2 (plastid transcriptionally active 9 )

HSP 1 Score: 97.4 bits (241), Expect = 2.0e-20
Identity = 65/213 (30.52%), Postives = 104/213 (48.83%), Query Frame = 0

Query: 70  PKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTETS-WINLTFW 129
           P+P+EI ++ E++N V+LIG V  PV+ +  + GK  A T ++   +S  +  WI + F 
Sbjct: 86  PRPNEIAYESEVANWVNLIGFVDQPVQFEASSDGKFWAGTVISQRSASDSSGFWIPIIFE 145

Query: 130 DELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLFHGFLFVRKVDA 189
            +LA  A+++V K +QI+VSG+L  D          TY +    +      F+       
Sbjct: 146 GDLAKTAARYVSKDDQIHVSGKLFID----SPPPNMTYAQANVQVLVQNLNFIQPMSPSP 205

Query: 190 GAFISFAGGRKLGSG--------AVDNNMGSTQELWQAFFANPVDWWDNRKNKKN----P 249
             F+  +   K  SG          D  +    + W     NP +WWD+R+NK N    P
Sbjct: 206 SPFMVMSSSEKEESGIKKQPGIAKQDIVIDEASDSWNHLIENPKEWWDHRENKVNGLVKP 265

Query: 250 KYPDFKHKDTGEALWVEGRYNPPWVKSQLALLD 270
           ++PDFK KD+  +LW+     P WV  +L  L+
Sbjct: 266 RHPDFKSKDSSFSLWLNKA--PNWVLPKLEGLE 292

BLAST of Lsi02G022440 vs. TAIR 10
Match: AT1G31010.1 (organellar single-stranded DNA binding protein 4 )

HSP 1 Score: 92.4 bits (228), Expect = 6.4e-19
Identity = 60/212 (28.30%), Postives = 105/212 (49.53%), Query Frame = 0

Query: 66  SVTYPKPSEIPWKKELSNSVSLIGTVGMPVELKHLASGKDVAWTRLAVWKSSTETS---- 125
           S  +P+P E+P++ +++NS+ LIG V  PV+      GK  A T ++   SS   S    
Sbjct: 56  SEEWPRPMEVPYQPKIANSIDLIGYVHQPVQFDSTLDGKFWAGTVISHEPSSDSKSESDS 115

Query: 126 ----WINLTFWDELAHVASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVLPSLFTLF 185
               WI + F  +LAH A+ +++K ++++++G+++ D++++     Q + ++  S    F
Sbjct: 116 SSNFWIPVLFEGDLAHTANSYLKKNDRVHITGQILGDVIQSGANSDQAHVQLFKSFHGSF 175

Query: 186 HGFLFVRKVDAGAFISFAGGRKLGSGAVDNNMG------STQ-------ELWQAFFANPV 245
              + VR +    +I  +         +D N G      S Q        LW      P 
Sbjct: 176 SHQVMVRDLH---YIEGSKAMPKVLPTLDQNEGVLKHSASVQRGREFGTNLWFDLVDKPN 235

Query: 246 DWWDNRKNKK----NPKYPDFKHKDTGEALWV 253
           +W D R+ K+    NPK+PDFK KD  +ALW+
Sbjct: 236 EWCDYREMKQNGSVNPKHPDFKKKDGSQALWL 264

BLAST of Lsi02G022440 vs. TAIR 10
Match: AT5G44785.2 (organellar single-stranded DNA binding protein 3 )

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-12
Identity = 29/53 (54.72%), Postives = 38/53 (71.70%), Query Frame = 0

Query: 213 QELWQAFFANPVDWWDNRKNKKNPKYPDFKHKDTGEALWVEGRYNPPWVKSQL 266
           +E+W+    NP  WWDNR +K+NPK PDFKHK+TGEALW+    +P W  S+L
Sbjct: 379 EEIWKNLVENPSKWWDNRLDKRNPKGPDFKHKETGEALWIGD--SPTWALSKL 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX993.2e-2331.86Protein OSB1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB1 PE=1 SV=1[more]
Q8GXH31.3e-1930.52Protein OSB2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB2 PE=1 SV=2[more]
Q9FYJ29.0e-1828.30Protein OSB4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB4 PE=2 SV=1[more]
Q8GWJ42.1e-1154.72Protein OSB3, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB... [more]
P0AGE21.6e-0625.00Single-stranded DNA-binding protein OS=Escherichia coli O157:H7 OX=83334 GN=ssb ... [more]
Match NameE-valueIdentityDescription
A0A1S3BRV34.3e-13281.06protein OSB2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492617 PE=4 SV... [more]
A0A5D3DR471.6e-13180.73Protein OSB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00530 P... [more]
A0A0A0K4W21.1e-13080.40Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G378540 PE=4 SV=1[more]
A0A6J1DQC62.1e-12678.23protein OSB2, chloroplastic-like isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1DNW71.9e-12477.89protein OSB2, chloroplastic-like isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_038896738.12.9e-13884.62protein OSB2, chloroplastic-like [Benincasa hispida][more]
XP_008451268.18.9e-13281.06PREDICTED: protein OSB2, chloroplastic-like [Cucumis melo][more]
KAA0059696.13.4e-13180.73protein OSB2 [Cucumis melo var. makuwa] >TYK26133.1 protein OSB2 [Cucumis melo v... [more]
XP_004146958.12.2e-13080.40protein OSB2, chloroplastic isoform X1 [Cucumis sativus] >KGN44765.1 hypothetica... [more]
XP_022155802.14.3e-12678.23protein OSB2, chloroplastic-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G47720.12.3e-2431.86Primosome PriB/single-strand DNA-binding [more]
AT4G20010.18.9e-2130.52plastid transcriptionally active 9 [more]
AT4G20010.22.0e-2030.52plastid transcriptionally active 9 [more]
AT1G31010.16.4e-1928.30organellar single-stranded DNA binding protein 4 [more]
AT5G44785.21.5e-1254.72organellar single-stranded DNA binding protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000424Primosome PriB/single-strand DNA-bindingPFAMPF00436SSBcoord: 83..169
e-value: 2.5E-15
score: 56.4
IPR000424Primosome PriB/single-strand DNA-bindingPROSITEPS50935SSBcoord: 81..178
score: 19.114252
IPR000424Primosome PriB/single-strand DNA-bindingCDDcd04496SSB_OBFcoord: 85..169
e-value: 1.80186E-17
score: 74.1857
NoneNo IPR availableGENE3D2.40.50.140coord: 81..218
e-value: 6.8E-18
score: 67.0
NoneNo IPR availablePANTHERPTHR10302:SF15OS03G0633900 PROTEINcoord: 23..285
IPR011344Single-stranded DNA-binding proteinPANTHERPTHR10302SINGLE-STRANDED DNA-BINDING PROTEINcoord: 23..285
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 83..264

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G022440.1Lsi02G022440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006264 mitochondrial DNA replication
biological_process GO:0051096 positive regulation of helicase activity
biological_process GO:0006260 DNA replication
cellular_component GO:0042645 mitochondrial nucleoid
molecular_function GO:0003697 single-stranded DNA binding