Lsi02G022330 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G022330
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionWRKY protein
Locationchr02: 28762750 .. 28765166 (+)
RNA-Seq ExpressionLsi02G022330
SyntenyLsi02G022330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTGCTTCTCTCATCATTTCATTAAAAAAATTTTACTCAAAACAAAAATCCCTTCTCTCTTCTCTCTTTCATTTCTCAATTTCTTCTCCTTCCCATTGTTGAATCAATCAAAATTTCTTCTAATCTTCCTCTTTCTCTTACTTCCCTTTTTCTTTTCTCTCTCCACAGATCATTTTTTCTGAAAAGAAAAAAAAAAAACAGAAAATTTAAAGAAAAGGAGAAAGAGAAAGATGGATGGATGGATGATGTGTGCTTTTTATTCTTTTGGGTCTCTCAAAATTCGTTTCAGTTGTATACTTTATTAATTCAATGGCAGAAGGAAGAATCTAGGGTTCCCATCCTCTGTTTTTCGATCCTGATTTTCCCTCAACCCTCTCTTTTCTTCTTCTTCTTGATCGTCTCTTTCTTTGTTATGCTTAATTTCAGCTAGGTTTTTTAGATTTCGCGATTTTGCTCATTTGGGTCTTCTCTTTTTAGGAAGATGATGGAAGAGGTTGAAGAAGCTAATAGGGAAGCTGTTGAGAGTTGTCATAAAGTGTTGAATCTGTTGACTGTGGCTTCCTCGTCTCAAGATCAGTTGAAGCTTAGGAGTTGTTTAATGGCGGAAACTGGAGAGGCTGTGTTTAAGTTTAGGAAAGTTTTGTGTCTTCTTGATTCTTCTGGGTTGGGTCATGCAAGGGTCAGGAAAAAGAAGGTCAAGAACTTTTTGTTTAATTCCTCTTCTTCCACTTTTCCTCTTCCTCAATCTATGTTCTTAGAAACTTCTTCCCCTAATTGCAGAATGGACCTTCTCCAGGGCAGGAATTTGCAGATGGGTCCTCTTTGTTTGGGGAACCCATCTTTGGAATTGAACTCAAATGCCAAAAGTTGTTCAATCCAACAAATACAATCTCAATCTGCTGCTCTTTATCATCATCATCATCATCATCATCACTTGCTTCAGAACAGGGTGTTGTTGAACAATAATCCTAATCCTAATCCTAATCCTAATCCTCCACAGCCTGAAATGGTGTATCTTAGGAGCAATAATGGCATTAACTTGAATTTTGATAGCTCTAGCTGCACACAACACACAATGTCATCCACTAGGTCGTTTATTTCTTCCTTGAGCATTGATGGGAGTGTGGCTAACTTGGATGGGAGTGCCTTTCATTTGATCGGGGCGCCCCGGTCTTCCGATCAGAATTCGCATCATAAGAGGAAGTGCAGTGGGAGAGGGGAAGATGGGAGTGTTAAATGTGGAAGCAGTGGGAGATGTCACTGCTCAAAAAAGAGGTTGGGAAGCTGTGGTTTTGCTTATGATGTCTTTAGGGGGATCTTTTGATATGTTTTTCTGATTCGATTTTGATAATGTTCTTGCAGGAAACATAGAGTAAAGCGATCGATTAAGGTGCCCGCCATAAGTAACAAGCTTGCAGATATCCCTCCTGATAATTATTCATGGAGAAAATATGGCCAAAAGCCAATCAAGGGTTCTCCTCATCCAAGGTATACAAATGCCATTTTACTACTTTTCAGCTTATTGTGTTGGTATTGCTTATTTCTTTATAGATTGATATTGGTTTTCATGATGTTATGCAAAAGATGTTGATTATATTCATTTGGCTGGCAATACTCTGAGCTGATCAATTATATGAATTCATAGGTTGTCAACTTGCCACATCTTTTCTGTTTTTTTGATTTGGGAAAGGGATTGTATGATTGCATCTATGGAATATTTCTAATCTTTCTTTTGGCATTACTTTGTGATTGAAAGATTCCTTGTCTGGTAATCTAATTGGGATATTGAAAACTTGCTCTATTTGTCTGCTCGTGTTCTCGAAATAGAATACGCCATTGAATTCTTTGCCGAGGTGGGGGACTTTAATGTTGTTCATTTGTCAAGTTGCATTGTGTACTGTATGCATATAGCAAATTGTTATGAGGGGTAGGCATACCGTCATAAAAGCTTCCTTGACCAATTGGATAGATCAAGAAAACAGCAGGGTACATCCCATTCTGCAAAATACTTTAAACATGGCTATTGAAACTTCAACCTGAAATTTGGAAACTGGTTGAAGAAATTTGTTAGAGTTCCACAGATTCTCCATGATTTGATATCGTATATACTTTGTTCCTTCGTGCCTATCTTAATTTACTTTCAAACATCTGCAGAGGATACTATAAGTGCAGCAGCATGAGAGGCTGTCCAGCAAGGAAGCACGTCGAGCGGTGTCTTGAGGAACCTTCAATGCTCATTGTTACATATGAAGGTGAACACAACCATCCTAAAATACCTTCACAACCTGCAAATACTTGAACCCCATGAAGACAACTACCCGAAACAGATTTGAAAACCTCCGTGCAATGTAGCGGAACGGCGACAAGCGTCCCGAGTGTCGCTGTACATATGGAATACTTTTGGAGCAGCTTTGTAG

mRNA sequence

GCTGCTTCTCTCATCATTTCATTAAAAAAATTTTACTCAAAACAAAAATCCCTTCTCTCTTCTCTCTTTCATTTCTCAATTTCTTCTCCTTCCCATTGTTGAATCAATCAAAATTTCTTCTAATCTTCCTCTTTCTCTTACTTCCCTTTTTCTTTTCTCTCTCCACAGATCATTTTTTCTGAAAAGAAAAAAAAAAAACAGAAAATTTAAAGAAAAGGAGAAAGAGAAAGATGGATGGATGGATGATGTGTGCTTTTTATTCTTTTGGGTCTCTCAAAATTCGTTTCAGTTGTATACTTTATTAATTCAATGGCAGAAGGAAGAATCTAGGGTTCCCATCCTCTGTTTTTCGATCCTGATTTTCCCTCAACCCTCTCTTTTCTTCTTCTTCTTGATCGTCTCTTTCTTTGTTATGCTTAATTTCAGCTAGGTTTTTTAGATTTCGCGATTTTGCTCATTTGGGTCTTCTCTTTTTAGGAAGATGATGGAAGAGGTTGAAGAAGCTAATAGGGAAGCTGTTGAGAGTTGTCATAAAGTGTTGAATCTGTTGACTGTGGCTTCCTCGTCTCAAGATCAGTTGAAGCTTAGGAGTTGTTTAATGGCGGAAACTGGAGAGGCTGTGTTTAAGTTTAGGAAAGTTTTGTGTCTTCTTGATTCTTCTGGGTTGGGTCATGCAAGGGTCAGGAAAAAGAAGGTCAAGAACTTTTTGTTTAATTCCTCTTCTTCCACTTTTCCTCTTCCTCAATCTATGTTCTTAGAAACTTCTTCCCCTAATTGCAGAATGGACCTTCTCCAGGGCAGGAATTTGCAGATGGGTCCTCTTTGTTTGGGGAACCCATCTTTGGAATTGAACTCAAATGCCAAAAGTTGTTCAATCCAACAAATACAATCTCAATCTGCTGCTCTTTATCATCATCATCATCATCATCATCACTTGCTTCAGAACAGGGTGTTGTTGAACAATAATCCTAATCCTAATCCTAATCCTAATCCTCCACAGCCTGAAATGGTGTATCTTAGGAGCAATAATGGCATTAACTTGAATTTTGATAGCTCTAGCTGCACACAACACACAATGTCATCCACTAGGTCGTTTATTTCTTCCTTGAGCATTGATGGGAGTGTGGCTAACTTGGATGGGAGTGCCTTTCATTTGATCGGGGCGCCCCGGTCTTCCGATCAGAATTCGCATCATAAGAGGAAGTGCAGTGGGAGAGGGGAAGATGGGAGTGTTAAATGTGGAAGCAGTGGGAGATGTCACTGCTCAAAAAAGAGGAAACATAGAGTAAAGCGATCGATTAAGGTGCCCGCCATAAGTAACAAGCTTGCAGATATCCCTCCTGATAATTATTCATGGAGAAAATATGGCCAAAAGCCAATCAAGGGTTCTCCTCATCCAAGAGGATACTATAAGTGCAGCAGCATGAGAGGCTGTCCAGCAAGGAAGCACGTCGAGCGGTGTCTTGAGGAACCTTCAATGCTCATTGTTACATATGAAGCGGAACGGCGACAAGCGTCCCGAGTGTCGCTGTACATATGGAATACTTTTGGAGCAGCTTTGTAG

Coding sequence (CDS)

ATGATGGAAGAGGTTGAAGAAGCTAATAGGGAAGCTGTTGAGAGTTGTCATAAAGTGTTGAATCTGTTGACTGTGGCTTCCTCGTCTCAAGATCAGTTGAAGCTTAGGAGTTGTTTAATGGCGGAAACTGGAGAGGCTGTGTTTAAGTTTAGGAAAGTTTTGTGTCTTCTTGATTCTTCTGGGTTGGGTCATGCAAGGGTCAGGAAAAAGAAGGTCAAGAACTTTTTGTTTAATTCCTCTTCTTCCACTTTTCCTCTTCCTCAATCTATGTTCTTAGAAACTTCTTCCCCTAATTGCAGAATGGACCTTCTCCAGGGCAGGAATTTGCAGATGGGTCCTCTTTGTTTGGGGAACCCATCTTTGGAATTGAACTCAAATGCCAAAAGTTGTTCAATCCAACAAATACAATCTCAATCTGCTGCTCTTTATCATCATCATCATCATCATCATCACTTGCTTCAGAACAGGGTGTTGTTGAACAATAATCCTAATCCTAATCCTAATCCTAATCCTCCACAGCCTGAAATGGTGTATCTTAGGAGCAATAATGGCATTAACTTGAATTTTGATAGCTCTAGCTGCACACAACACACAATGTCATCCACTAGGTCGTTTATTTCTTCCTTGAGCATTGATGGGAGTGTGGCTAACTTGGATGGGAGTGCCTTTCATTTGATCGGGGCGCCCCGGTCTTCCGATCAGAATTCGCATCATAAGAGGAAGTGCAGTGGGAGAGGGGAAGATGGGAGTGTTAAATGTGGAAGCAGTGGGAGATGTCACTGCTCAAAAAAGAGGAAACATAGAGTAAAGCGATCGATTAAGGTGCCCGCCATAAGTAACAAGCTTGCAGATATCCCTCCTGATAATTATTCATGGAGAAAATATGGCCAAAAGCCAATCAAGGGTTCTCCTCATCCAAGAGGATACTATAAGTGCAGCAGCATGAGAGGCTGTCCAGCAAGGAAGCACGTCGAGCGGTGTCTTGAGGAACCTTCAATGCTCATTGTTACATATGAAGCGGAACGGCGACAAGCGTCCCGAGTGTCGCTGTACATATGGAATACTTTTGGAGCAGCTTTGTAG

Protein sequence

MMEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSGLGHARVRKKKVKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQGRNLQMGPLCLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQPEMVYLRSNNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAERRQASRVSLYIWNTFGAAL
Homology
BLAST of Lsi02G022330 vs. ExPASy Swiss-Prot
Match: O04336 (Probable WRKY transcription factor 21 OS=Arabidopsis thaliana OX=3702 GN=WRKY21 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.4e-81
Identity = 193/381 (50.66%), Postives = 235/381 (61.68%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEE+E  NR AVESCH+VLNLL   S  QD +     L++ET EAV +F++V  LL SS 
Sbjct: 1   MEEIEGTNRAAVESCHRVLNLLH-RSQQQDHVGFEKNLVSETREAVIRFKRVGSLL-SSS 60

Query: 62  LGHARVRKKKVKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQGRNLQMG------PLC 121
           +GHAR R+ K      + S    P  Q      SS + +  +L+    ++        L 
Sbjct: 61  VGHARFRRAKKLQSHVSQSLLLDPCQQRTTEVPSSSSQKTPVLRSGFQELSLRQPSDSLT 120

Query: 122 LGNPSLELNSNAKSCSI---------------------------------QQIQSQSAAL 181
           LG  S  LNSNAK+  +                                 QQ Q Q    
Sbjct: 121 LGTRSFSLNSNAKAPLLQLNQQTMPPSNYPTLFPVQQQQQQQQQQQQQEQQQQQQQQQQQ 180

Query: 182 YHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQPEMVYLRSNNGINLNFDSSSCTQHTMSST 241
           +H     HHL Q + L  +           Q E++  + N GI+L+FD+SSCT  TMSST
Sbjct: 181 FHERLQAHHLHQQQQLQKH-----------QAELMLRKCNGGISLSFDNSSCTP-TMSST 240

Query: 242 RSFISSLSIDGSVANLDGSAFHLIGAPRSSDQNS-HHKRKCSGRG-EDGSVKCGSSGRCH 301
           RSF+SSLSIDGSVAN++G      G P S+DQNS H KRKC  +G E GS+KCGSS RCH
Sbjct: 241 RSFVSSLSIDGSVANIEGKNSFHFGVPSSTDQNSLHSKRKCPLKGDEHGSLKCGSSSRCH 300

Query: 302 CSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPA 342
           C+KKRKHRV+RSI+VPAISNK+ADIPPD+YSWRKYGQKPIKGSP+PRGYYKCSSMRGCPA
Sbjct: 301 CAKKRKHRVRRSIRVPAISNKVADIPPDDYSWRKYGQKPIKGSPYPRGYYKCSSMRGCPA 360

BLAST of Lsi02G022330 vs. ExPASy Swiss-Prot
Match: Q93WU6 (Probable WRKY transcription factor 74 OS=Arabidopsis thaliana OX=3702 GN=WRKY74 PE=2 SV=2)

HSP 1 Score: 277.3 bits (708), Expect = 2.4e-73
Identity = 180/364 (49.45%), Postives = 221/364 (60.71%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVE AN+ AVESCH VLNLL+       Q      +M ET EAV KF++V  LL S G
Sbjct: 1   MEEVEAANKAAVESCHGVLNLLS------QQTNDSKSIMVETREAVCKFKRVSSLL-SRG 60

Query: 62  LGHARVRKKKVKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQG-------RNLQM--- 121
           LG  +++K    N+ F+SS     LPQ MFLE  SP C  + + G       + LQ+   
Sbjct: 61  LGQRKIKKLNNNNYKFSSSL----LPQHMFLE--SPVCSNNAISGCIPILAPKPLQIVPA 120

Query: 122 --GPLCLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNP 181
              PL L N ++ L+ +         ++     Y   H H                    
Sbjct: 121 GPPPLMLFNQNMCLDKSFLELKPPSSRAVDPKPYQFIHTHQ------------------- 180

Query: 182 NPPQPEMVYLRSNNGINLNFDSS---SCTQHTMSS-TRSFISSLSIDGSVANLDGSAFHL 241
                + VY RS +G+NL FD S   SC   ++S+ +RSF+SSLS+DGSV + D ++FHL
Sbjct: 181 -----QGVYSRSKSGLNLKFDGSIGASCYSPSISNGSRSFVSSLSMDGSVTDYDRNSFHL 240

Query: 242 IGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADI 301
           IG P+ SD  S H R+ S     GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADI
Sbjct: 241 IGLPQGSDHISQHSRRTSC---SGSLKCGSKSKCHCSKKRKLRVKRSIKVPAISNKIADI 300

Query: 302 PPDNYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAERRQA 350
           PPD YSWRKYGQKPIKGSPHPRGYYKCSS+RGCPARKHVERC+EE SMLIVTYE E   +
Sbjct: 301 PPDEYSWRKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERCVEETSMLIVTYEGEHNHS 324

BLAST of Lsi02G022330 vs. ExPASy Swiss-Prot
Match: Q9SR07 (Probable WRKY transcription factor 39 OS=Arabidopsis thaliana OX=3702 GN=WRKY39 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 9.5e-70
Identity = 177/357 (49.58%), Postives = 216/357 (60.50%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVE ANR A+ESCH VLNLL+  +S    L +      ETGE V KF++V  LL + G
Sbjct: 1   MEEVEAANRSAIESCHGVLNLLSQRTSDPKSLTV------ETGEVVSKFKRVASLL-TRG 60

Query: 62  LGHARVRKKKVKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQGRNLQM---GPLCLGN 121
           LGH + R         N   S+F  PQ +FLE  SP C  + L G   Q+    PL +  
Sbjct: 61  LGHGKFRST-------NKFRSSF--PQHIFLE--SPICCGNDLSGDYTQVLAPEPLQMVP 120

Query: 122 PSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNP---PQPE 181
            S   N         Q+   S  L       H +  ++  L   P P   P        +
Sbjct: 121 ASAVYNEMEPK---HQLGHPSLML------SHKMCVDKSFLELKPPPFRAPYQLIHNHQQ 180

Query: 182 MVYLRSNNGINLNFDSSSCTQHTMS---STRSFISSLSIDGSVANLDGSAFHLIGAPRSS 241
           + Y RSN+G+NL FD S  + +T S    +RSF+SSLS+D SV + D ++FHL G  R S
Sbjct: 181 IAYSRSNSGVNLKFDGSGSSCYTPSVSNGSRSFVSSLSMDASVTDYDRNSFHLTGLSRGS 240

Query: 242 DQNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSW 301
           DQ  H ++ CS     GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADIPPD YSW
Sbjct: 241 DQ-QHTRKMCS-----GSLKCGSRSKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSW 300

Query: 302 RKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAERRQASRVS 350
           RKYGQKPIKGSPHPRGYYKCSS+RGCPARKHVERC++E SMLIVTYE E   +  +S
Sbjct: 301 RKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERCIDETSMLIVTYEGEHNHSRILS 324

BLAST of Lsi02G022330 vs. ExPASy Swiss-Prot
Match: Q32SG4 (Protein WRKY1 OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.0e-56
Identity = 165/393 (41.98%), Postives = 219/393 (55.73%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSS- 61
           ME +EEANR AVESCH+VL LL   S+   QL     L+A TGEAV KF  +   L +S 
Sbjct: 1   MEGMEEANRTAVESCHRVLALL---SNPHGQLVPSKELVAATGEAVAKFGSLTAKLSNSN 60

Query: 62  --GL--GHARVRKKKVKNFLFNS-----SSSTFPLPQSMFLETSSPNCRMDLLQGRNLQM 121
             GL  GHARVRK K    +F+S     SS+           + SP   + L    +   
Sbjct: 61  GDGLLQGHARVRKVKKPLHIFDSNLFLESSAVAAAAAPAKTPSPSPILGLQLFPRYHQFE 120

Query: 122 GP---------------LCLGNPS--LELNSNAKSCSIQQIQSQSAA------------- 181
           G                L L  P+  +E +++     +Q +Q  S A             
Sbjct: 121 GSSSKDPVRIPTQFPKRLLLEKPTAGMEGSTSQSPPIVQMVQPVSVAPPAGTPTPALPPA 180

Query: 182 ----LYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQPEMV--YLRSNNGINLNFDSSSCT 241
               +     +    L  ++ + +      N       +         G+NL FDSS+C 
Sbjct: 181 HLHFIQQQQSYQRFQLMQQMKIQSEMMKRSNLGDQGGSLSGGGGGGRKGVNLKFDSSNC- 240

Query: 242 QHTMSSTRSFISSLSIDGSVANLDGS----AFHLIGAPRSS---DQNSHHKRKCSGRGED 301
             T SS+RSF+SSLS++GS+A+LDGS     F L+   +++   +     +R+C+GR ED
Sbjct: 241 --TASSSRSFLSSLSMEGSLASLDGSRTSRPFQLLSGSQTASTPELGLVQRRRCAGR-ED 300

Query: 302 GSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRKYGQKPIKGSPHPRG 342
           G+ +C +  RCHCSKKRK R++RSIKVPAISNK+ADIP D +SWRKYGQKPIKGSPHPRG
Sbjct: 301 GTGRCATGSRCHCSKKRKLRIRRSIKVPAISNKVADIPADEFSWRKYGQKPIKGSPHPRG 360

BLAST of Lsi02G022330 vs. ExPASy Swiss-Prot
Match: Q9SJA8 (Probable WRKY transcription factor 17 OS=Arabidopsis thaliana OX=3702 GN=WRKY17 PE=1 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 4.3e-38
Identity = 85/159 (53.46%), Postives = 111/159 (69.81%), Query Frame = 0

Query: 198 TMSSTRSFISS-LSIDGSVANLDGSAFHLIGAPRSSDQNS--------HHKRKC----SG 257
           ++SS  SF+SS ++ DGSV+   GS+  L  AP     +S         ++++C      
Sbjct: 143 SVSSNSSFMSSAITGDGSVSK--GSSIFLAPAPAVPVTSSGKPPLSGLPYRKRCFEHDHS 202

Query: 258 RGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRKYGQKPIKGSP 317
            G  G +    +G+CHC K RK+R+KR+++VPA+S K+ADIPPD YSWRKYGQKPIKGSP
Sbjct: 203 EGFSGKISGSGNGKCHCKKSRKNRMKRTVRVPAVSAKIADIPPDEYSWRKYGQKPIKGSP 262

Query: 318 HPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAERR 344
           HPRGYYKCS+ RGCPARKHVER L++ +MLIVTYE E R
Sbjct: 263 HPRGYYKCSTFRGCPARKHVERALDDSTMLIVTYEGEHR 299

BLAST of Lsi02G022330 vs. ExPASy TrEMBL
Match: E7CEY5 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY51 PE=2 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.4e-169
Identity = 312/347 (89.91%), Postives = 326/347 (93.95%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVEEANREAVESCHKVLNLLT+ SSSQD LKLRSCLMAETGEAVFKFRKVLCLLDSSG
Sbjct: 1   MEEVEEANREAVESCHKVLNLLTLPSSSQDHLKLRSCLMAETGEAVFKFRKVLCLLDSSG 60

Query: 62  LGHARVR-KKKVKNFLFNSSSST------FPLPQSMFLETSSPNCRMDLLQGRNLQMGPL 121
           LGHARVR KKKV NFLFNSSSS+      FPLPQS+FLET SP+CRMD LQGRNLQMGPL
Sbjct: 61  LGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETYSPDCRMDHLQGRNLQMGPL 120

Query: 122 CLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQP 181
           CLGNPSLELN+NAK+CSIQQIQSQSAALY  HHHHHHLLQNRV+LNN      NPNPPQP
Sbjct: 121 CLGNPSLELNTNAKTCSIQQIQSQSAALY--HHHHHHLLQNRVVLNN------NPNPPQP 180

Query: 182 EMVYLRSNNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ 241
           E+VYLRS+NG+NLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ
Sbjct: 181 EVVYLRSSNGVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ 240

Query: 242 NSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRK 301
           NS+HKRKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPD+YSWRK
Sbjct: 241 NSYHKRKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRK 300

Query: 302 YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 339

BLAST of Lsi02G022330 vs. ExPASy TrEMBL
Match: A0A5D3DSD0 (Putative WRKY transcription factor 21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00720 PE=4 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.4e-169
Identity = 314/348 (90.23%), Postives = 326/348 (93.68%), Query Frame = 0

Query: 1   MMEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSS 60
           MMEEVE+ANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEA+FKFRKVLCLLDSS
Sbjct: 1   MMEEVEQANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEALFKFRKVLCLLDSS 60

Query: 61  GLGHARVR-KKKVKNFLFNSSSST------FPLPQSMFLETSSPNCRMDLLQGRNLQMGP 120
           GLGHARVR KKKV NFLFNSSSS+      FPLPQS+FLETSSP+CRMDLLQ RNLQMGP
Sbjct: 61  GLGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETSSPDCRMDLLQCRNLQMGP 120

Query: 121 LCLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQ 180
           LCLGNPSLELN+NAKSCSIQQIQSQSAALY     HHHLLQNRV+LNN      NPNPPQ
Sbjct: 121 LCLGNPSLELNTNAKSCSIQQIQSQSAALY-----HHHLLQNRVVLNN------NPNPPQ 180

Query: 181 PEMVYLRSNNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD 240
            EMVYLRSNNG+NLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD
Sbjct: 181 HEMVYLRSNNGVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD 240

Query: 241 QNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWR 300
           QNS+HKRKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPD+YSWR
Sbjct: 241 QNSYHKRKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR 300

Query: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 337

BLAST of Lsi02G022330 vs. ExPASy TrEMBL
Match: N1K0K3 (WRKY transcription factor 21 OS=Cucumis sativus OX=3659 GN=WRKY-21 PE=2 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 7.1e-169
Identity = 311/347 (89.63%), Postives = 325/347 (93.66%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVEEANREAVESCHKVLNLLT+ SSSQD LKLRSCLMAETGEA FKFRKVLCLLDSSG
Sbjct: 1   MEEVEEANREAVESCHKVLNLLTLPSSSQDHLKLRSCLMAETGEAAFKFRKVLCLLDSSG 60

Query: 62  LGHARVR-KKKVKNFLFNSSSST------FPLPQSMFLETSSPNCRMDLLQGRNLQMGPL 121
           LGHARVR KKKV NFLFNSSSS+      FPLPQS+FLET SP+CRMD LQGRNLQMGPL
Sbjct: 61  LGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETYSPDCRMDHLQGRNLQMGPL 120

Query: 122 CLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQP 181
           CLGNPSLELN+NAK+CSIQQIQSQSAALY  HHHHHHLLQNRV+LNN      NPNPPQP
Sbjct: 121 CLGNPSLELNTNAKTCSIQQIQSQSAALY--HHHHHHLLQNRVVLNN------NPNPPQP 180

Query: 182 EMVYLRSNNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ 241
           E+VYLRS+NG+NLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ
Sbjct: 181 EVVYLRSSNGVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ 240

Query: 242 NSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRK 301
           NS+HKRKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPD+YSWRK
Sbjct: 241 NSYHKRKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRK 300

Query: 302 YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 339

BLAST of Lsi02G022330 vs. ExPASy TrEMBL
Match: A0A1S3BQJ0 (LOW QUALITY PROTEIN: probable WRKY transcription factor 21 OS=Cucumis melo OX=3656 GN=LOC103492629 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.1e-168
Identity = 313/348 (89.94%), Postives = 325/348 (93.39%), Query Frame = 0

Query: 1   MMEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSS 60
           MMEEVE+ANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEA+FKFRKVLCLLDSS
Sbjct: 1   MMEEVEQANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEALFKFRKVLCLLDSS 60

Query: 61  GLGHARVR-KKKVKNFLFNSSSST------FPLPQSMFLETSSPNCRMDLLQGRNLQMGP 120
           GLGHARVR KKKV NFLFNSSSS+      FPLPQS+FLETSSP+CRMDLLQ RNL MGP
Sbjct: 61  GLGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETSSPDCRMDLLQCRNLHMGP 120

Query: 121 LCLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQ 180
           LCLGNPSLELN+NAKSCSIQQIQSQSAALY     HHHLLQNRV+LNN      NPNPPQ
Sbjct: 121 LCLGNPSLELNTNAKSCSIQQIQSQSAALY-----HHHLLQNRVVLNN------NPNPPQ 180

Query: 181 PEMVYLRSNNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD 240
            EMVYLRSNNG+NLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD
Sbjct: 181 HEMVYLRSNNGVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD 240

Query: 241 QNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWR 300
           QNS+HKRKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPD+YSWR
Sbjct: 241 QNSYHKRKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR 300

Query: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 337

BLAST of Lsi02G022330 vs. ExPASy TrEMBL
Match: A0A2S0USY9 (WRKY transcription factor 21-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 4.2e-161
Identity = 302/340 (88.82%), Postives = 314/340 (92.35%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVEEANR+AVESCH+VLNLLTVA S QD  KLRSCLMAETG+AVFKFRKVLCLLDSSG
Sbjct: 1   MEEVEEANRDAVESCHRVLNLLTVA-SPQDHSKLRSCLMAETGQAVFKFRKVLCLLDSSG 60

Query: 62  LGHARVRKKKVKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQGRNLQMGPLCLGNPSL 121
           LGHARVRKKKVK+F FN SSS FPLPQSMFLET SPNCRMDLLQGRNLQMGPL LG+PSL
Sbjct: 61  LGHARVRKKKVKSFQFN-SSSPFPLPQSMFLETFSPNCRMDLLQGRNLQMGPLSLGSPSL 120

Query: 122 ELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQPEMVYLRS 181
           EL+SN KSCSIQQ Q Q        H+HHH LQNRVLLN+NPN NPN +  QPEMVYLRS
Sbjct: 121 ELSSNGKSCSIQQQQQQQPQSV--GHYHHHFLQNRVLLNSNPNINPNAS--QPEMVYLRS 180

Query: 182 NNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQNSHHKRK 241
           N+GINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQNSHHKRK
Sbjct: 181 NSGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQNSHHKRK 240

Query: 242 CSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRKYGQKPIK 301
           CSGRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPD+YSWRKYGQKPIK
Sbjct: 241 CSGRGEDGSVKCGSTGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKPIK 300

Query: 302 GSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           GSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 GSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 334

BLAST of Lsi02G022330 vs. NCBI nr
Match: XP_038899952.1 (probable WRKY transcription factor 74 isoform X1 [Benincasa hispida])

HSP 1 Score: 618.6 bits (1594), Expect = 3.4e-173
Identity = 322/342 (94.15%), Postives = 325/342 (95.03%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVEEAN+EAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG
Sbjct: 1   MEEVEEANKEAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 60

Query: 62  LGHARVRKKK-VKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQGRNLQMGPLCLGNPS 121
           LGHARVRKKK VKNFLFNSSSS FPLPQSMFLETSSPNCRMDLLQGRNLQ GPLCLG PS
Sbjct: 61  LGHARVRKKKVVKNFLFNSSSSHFPLPQSMFLETSSPNCRMDLLQGRNLQTGPLCLGKPS 120

Query: 122 LELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQPEMVYLR 181
           LELN N KSCSIQQIQSQSAALY     HHHLLQNR LLNN  NPNPNPNPPQPEMVYLR
Sbjct: 121 LELNPNGKSCSIQQIQSQSAALY-----HHHLLQNRALLNN--NPNPNPNPPQPEMVYLR 180

Query: 182 SN-NGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQNSHHK 241
           SN NGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQNS+HK
Sbjct: 181 SNSNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQNSYHK 240

Query: 242 RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRKYGQKP 301
           RKCSGRGEDGSVKCGSSG+CHCSKKRKHRVKRSIKVPAISNKLADIPPD YSWRKYGQKP
Sbjct: 241 RKCSGRGEDGSVKCGSSGKCHCSKKRKHRVKRSIKVPAISNKLADIPPDYYSWRKYGQKP 300

Query: 302 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 IKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 335

BLAST of Lsi02G022330 vs. NCBI nr
Match: XP_004146948.2 (probable WRKY transcription factor 21 [Cucumis sativus] >ADU52528.1 WRKY protein [Cucumis sativus])

HSP 1 Score: 604.7 bits (1558), Expect = 5.0e-169
Identity = 312/347 (89.91%), Postives = 326/347 (93.95%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVEEANREAVESCHKVLNLLT+ SSSQD LKLRSCLMAETGEAVFKFRKVLCLLDSSG
Sbjct: 1   MEEVEEANREAVESCHKVLNLLTLPSSSQDHLKLRSCLMAETGEAVFKFRKVLCLLDSSG 60

Query: 62  LGHARVR-KKKVKNFLFNSSSST------FPLPQSMFLETSSPNCRMDLLQGRNLQMGPL 121
           LGHARVR KKKV NFLFNSSSS+      FPLPQS+FLET SP+CRMD LQGRNLQMGPL
Sbjct: 61  LGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETYSPDCRMDHLQGRNLQMGPL 120

Query: 122 CLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQP 181
           CLGNPSLELN+NAK+CSIQQIQSQSAALY  HHHHHHLLQNRV+LNN      NPNPPQP
Sbjct: 121 CLGNPSLELNTNAKTCSIQQIQSQSAALY--HHHHHHLLQNRVVLNN------NPNPPQP 180

Query: 182 EMVYLRSNNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ 241
           E+VYLRS+NG+NLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ
Sbjct: 181 EVVYLRSSNGVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ 240

Query: 242 NSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRK 301
           NS+HKRKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPD+YSWRK
Sbjct: 241 NSYHKRKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRK 300

Query: 302 YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 339

BLAST of Lsi02G022330 vs. NCBI nr
Match: KAA0059712.1 (putative WRKY transcription factor 21 [Cucumis melo var. makuwa] >TYK26150.1 putative WRKY transcription factor 21 [Cucumis melo var. makuwa])

HSP 1 Score: 603.6 bits (1555), Expect = 1.1e-168
Identity = 314/348 (90.23%), Postives = 326/348 (93.68%), Query Frame = 0

Query: 1   MMEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSS 60
           MMEEVE+ANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEA+FKFRKVLCLLDSS
Sbjct: 1   MMEEVEQANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEALFKFRKVLCLLDSS 60

Query: 61  GLGHARVR-KKKVKNFLFNSSSST------FPLPQSMFLETSSPNCRMDLLQGRNLQMGP 120
           GLGHARVR KKKV NFLFNSSSS+      FPLPQS+FLETSSP+CRMDLLQ RNLQMGP
Sbjct: 61  GLGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETSSPDCRMDLLQCRNLQMGP 120

Query: 121 LCLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQ 180
           LCLGNPSLELN+NAKSCSIQQIQSQSAALY     HHHLLQNRV+LNN      NPNPPQ
Sbjct: 121 LCLGNPSLELNTNAKSCSIQQIQSQSAALY-----HHHLLQNRVVLNN------NPNPPQ 180

Query: 181 PEMVYLRSNNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD 240
            EMVYLRSNNG+NLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD
Sbjct: 181 HEMVYLRSNNGVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD 240

Query: 241 QNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWR 300
           QNS+HKRKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPD+YSWR
Sbjct: 241 QNSYHKRKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR 300

Query: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 337

BLAST of Lsi02G022330 vs. NCBI nr
Match: CCV01211.1 (WRKY transcription factor 21 [Cucumis sativus])

HSP 1 Score: 603.2 bits (1554), Expect = 1.5e-168
Identity = 311/347 (89.63%), Postives = 325/347 (93.66%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVEEANREAVESCHKVLNLLT+ SSSQD LKLRSCLMAETGEA FKFRKVLCLLDSSG
Sbjct: 1   MEEVEEANREAVESCHKVLNLLTLPSSSQDHLKLRSCLMAETGEAAFKFRKVLCLLDSSG 60

Query: 62  LGHARVR-KKKVKNFLFNSSSST------FPLPQSMFLETSSPNCRMDLLQGRNLQMGPL 121
           LGHARVR KKKV NFLFNSSSS+      FPLPQS+FLET SP+CRMD LQGRNLQMGPL
Sbjct: 61  LGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETYSPDCRMDHLQGRNLQMGPL 120

Query: 122 CLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQP 181
           CLGNPSLELN+NAK+CSIQQIQSQSAALY  HHHHHHLLQNRV+LNN      NPNPPQP
Sbjct: 121 CLGNPSLELNTNAKTCSIQQIQSQSAALY--HHHHHHLLQNRVVLNN------NPNPPQP 180

Query: 182 EMVYLRSNNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ 241
           E+VYLRS+NG+NLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ
Sbjct: 181 EVVYLRSSNGVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSDQ 240

Query: 242 NSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRK 301
           NS+HKRKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPD+YSWRK
Sbjct: 241 NSYHKRKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRK 300

Query: 302 YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 YGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 339

BLAST of Lsi02G022330 vs. NCBI nr
Match: XP_008451288.1 (PREDICTED: LOW QUALITY PROTEIN: probable WRKY transcription factor 21 [Cucumis melo])

HSP 1 Score: 601.7 bits (1550), Expect = 4.2e-168
Identity = 313/348 (89.94%), Postives = 325/348 (93.39%), Query Frame = 0

Query: 1   MMEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSS 60
           MMEEVE+ANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEA+FKFRKVLCLLDSS
Sbjct: 1   MMEEVEQANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEALFKFRKVLCLLDSS 60

Query: 61  GLGHARVR-KKKVKNFLFNSSSST------FPLPQSMFLETSSPNCRMDLLQGRNLQMGP 120
           GLGHARVR KKKV NFLFNSSSS+      FPLPQS+FLETSSP+CRMDLLQ RNL MGP
Sbjct: 61  GLGHARVRKKKKVNNFLFNSSSSSSSSSSPFPLPQSLFLETSSPDCRMDLLQCRNLHMGP 120

Query: 121 LCLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQ 180
           LCLGNPSLELN+NAKSCSIQQIQSQSAALY     HHHLLQNRV+LNN      NPNPPQ
Sbjct: 121 LCLGNPSLELNTNAKSCSIQQIQSQSAALY-----HHHLLQNRVVLNN------NPNPPQ 180

Query: 181 PEMVYLRSNNGINLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD 240
            EMVYLRSNNG+NLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD
Sbjct: 181 HEMVYLRSNNGVNLNFDSSSCTQHTMSSTRSFISSLSIDGSVANLDGSAFHLIGAPRSSD 240

Query: 241 QNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWR 300
           QNS+HKRKC+GRGEDGSVKCGS+GRCHCSKKRKHRVKRSIKVPAISNKLADIPPD+YSWR
Sbjct: 241 QNSYHKRKCNGRGEDGSVKCGSNGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWR 300

Query: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAE 342
           KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYE E
Sbjct: 301 KYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEGE 337

BLAST of Lsi02G022330 vs. TAIR 10
Match: AT2G30590.1 (WRKY DNA-binding protein 21 )

HSP 1 Score: 303.9 bits (777), Expect = 1.7e-82
Identity = 193/381 (50.66%), Postives = 235/381 (61.68%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEE+E  NR AVESCH+VLNLL   S  QD +     L++ET EAV +F++V  LL SS 
Sbjct: 1   MEEIEGTNRAAVESCHRVLNLLH-RSQQQDHVGFEKNLVSETREAVIRFKRVGSLL-SSS 60

Query: 62  LGHARVRKKKVKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQGRNLQMG------PLC 121
           +GHAR R+ K      + S    P  Q      SS + +  +L+    ++        L 
Sbjct: 61  VGHARFRRAKKLQSHVSQSLLLDPCQQRTTEVPSSSSQKTPVLRSGFQELSLRQPSDSLT 120

Query: 122 LGNPSLELNSNAKSCSI---------------------------------QQIQSQSAAL 181
           LG  S  LNSNAK+  +                                 QQ Q Q    
Sbjct: 121 LGTRSFSLNSNAKAPLLQLNQQTMPPSNYPTLFPVQQQQQQQQQQQQQEQQQQQQQQQQQ 180

Query: 182 YHHHHHHHHLLQNRVLLNNNPNPNPNPNPPQPEMVYLRSNNGINLNFDSSSCTQHTMSST 241
           +H     HHL Q + L  +           Q E++  + N GI+L+FD+SSCT  TMSST
Sbjct: 181 FHERLQAHHLHQQQQLQKH-----------QAELMLRKCNGGISLSFDNSSCTP-TMSST 240

Query: 242 RSFISSLSIDGSVANLDGSAFHLIGAPRSSDQNS-HHKRKCSGRG-EDGSVKCGSSGRCH 301
           RSF+SSLSIDGSVAN++G      G P S+DQNS H KRKC  +G E GS+KCGSS RCH
Sbjct: 241 RSFVSSLSIDGSVANIEGKNSFHFGVPSSTDQNSLHSKRKCPLKGDEHGSLKCGSSSRCH 300

Query: 302 CSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPA 342
           C+KKRKHRV+RSI+VPAISNK+ADIPPD+YSWRKYGQKPIKGSP+PRGYYKCSSMRGCPA
Sbjct: 301 CAKKRKHRVRRSIRVPAISNKVADIPPDDYSWRKYGQKPIKGSPYPRGYYKCSSMRGCPA 360

BLAST of Lsi02G022330 vs. TAIR 10
Match: AT5G28650.1 (WRKY DNA-binding protein 74 )

HSP 1 Score: 277.3 bits (708), Expect = 1.7e-74
Identity = 180/364 (49.45%), Postives = 221/364 (60.71%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVE AN+ AVESCH VLNLL+       Q      +M ET EAV KF++V  LL S G
Sbjct: 1   MEEVEAANKAAVESCHGVLNLLS------QQTNDSKSIMVETREAVCKFKRVSSLL-SRG 60

Query: 62  LGHARVRKKKVKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQG-------RNLQM--- 121
           LG  +++K    N+ F+SS     LPQ MFLE  SP C  + + G       + LQ+   
Sbjct: 61  LGQRKIKKLNNNNYKFSSSL----LPQHMFLE--SPVCSNNAISGCIPILAPKPLQIVPA 120

Query: 122 --GPLCLGNPSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNP 181
              PL L N ++ L+ +         ++     Y   H H                    
Sbjct: 121 GPPPLMLFNQNMCLDKSFLELKPPSSRAVDPKPYQFIHTHQ------------------- 180

Query: 182 NPPQPEMVYLRSNNGINLNFDSS---SCTQHTMSS-TRSFISSLSIDGSVANLDGSAFHL 241
                + VY RS +G+NL FD S   SC   ++S+ +RSF+SSLS+DGSV + D ++FHL
Sbjct: 181 -----QGVYSRSKSGLNLKFDGSIGASCYSPSISNGSRSFVSSLSMDGSVTDYDRNSFHL 240

Query: 242 IGAPRSSDQNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADI 301
           IG P+ SD  S H R+ S     GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADI
Sbjct: 241 IGLPQGSDHISQHSRRTSC---SGSLKCGSKSKCHCSKKRKLRVKRSIKVPAISNKIADI 300

Query: 302 PPDNYSWRKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAERRQA 350
           PPD YSWRKYGQKPIKGSPHPRGYYKCSS+RGCPARKHVERC+EE SMLIVTYE E   +
Sbjct: 301 PPDEYSWRKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERCVEETSMLIVTYEGEHNHS 324

BLAST of Lsi02G022330 vs. TAIR 10
Match: AT3G04670.1 (WRKY DNA-binding protein 39 )

HSP 1 Score: 265.4 bits (677), Expect = 6.7e-71
Identity = 177/357 (49.58%), Postives = 216/357 (60.50%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVE ANR A+ESCH VLNLL+  +S    L +      ETGE V KF++V  LL + G
Sbjct: 1   MEEVEAANRSAIESCHGVLNLLSQRTSDPKSLTV------ETGEVVSKFKRVASLL-TRG 60

Query: 62  LGHARVRKKKVKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQGRNLQM---GPLCLGN 121
           LGH + R         N   S+F  PQ +FLE  SP C  + L G   Q+    PL +  
Sbjct: 61  LGHGKFRST-------NKFRSSF--PQHIFLE--SPICCGNDLSGDYTQVLAPEPLQMVP 120

Query: 122 PSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNP---PQPE 181
            S   N         Q+   S  L       H +  ++  L   P P   P        +
Sbjct: 121 ASAVYNEMEPK---HQLGHPSLML------SHKMCVDKSFLELKPPPFRAPYQLIHNHQQ 180

Query: 182 MVYLRSNNGINLNFDSSSCTQHTMS---STRSFISSLSIDGSVANLDGSAFHLIGAPRSS 241
           + Y RSN+G+NL FD S  + +T S    +RSF+SSLS+D SV + D ++FHL G  R S
Sbjct: 181 IAYSRSNSGVNLKFDGSGSSCYTPSVSNGSRSFVSSLSMDASVTDYDRNSFHLTGLSRGS 240

Query: 242 DQNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSW 301
           DQ  H ++ CS     GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADIPPD YSW
Sbjct: 241 DQ-QHTRKMCS-----GSLKCGSRSKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSW 300

Query: 302 RKYGQKPIKGSPHPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAERRQASRVS 350
           RKYGQKPIKGSPHPRGYYKCSS+RGCPARKHVERC++E SMLIVTYE E   +  +S
Sbjct: 301 RKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERCIDETSMLIVTYEGEHNHSRILS 324

BLAST of Lsi02G022330 vs. TAIR 10
Match: AT3G04670.2 (WRKY DNA-binding protein 39 )

HSP 1 Score: 203.4 bits (516), Expect = 3.1e-52
Identity = 149/319 (46.71%), Postives = 183/319 (57.37%), Query Frame = 0

Query: 2   MEEVEEANREAVESCHKVLNLLTVASSSQDQLKLRSCLMAETGEAVFKFRKVLCLLDSSG 61
           MEEVE ANR A+ESCH VLNLL+  +S    L +      ETGE V KF++V  LL + G
Sbjct: 1   MEEVEAANRSAIESCHGVLNLLSQRTSDPKSLTV------ETGEVVSKFKRVASLL-TRG 60

Query: 62  LGHARVRKKKVKNFLFNSSSSTFPLPQSMFLETSSPNCRMDLLQGRNLQM---GPLCLGN 121
           LGH + R         N   S+F  PQ +FLE  SP C  + L G   Q+    PL +  
Sbjct: 61  LGHGKFRST-------NKFRSSF--PQHIFLE--SPICCGNDLSGDYTQVLAPEPLQMVP 120

Query: 122 PSLELNSNAKSCSIQQIQSQSAALYHHHHHHHHLLQNRVLLNNNPNPNPNPNP---PQPE 181
            S   N         Q+   S  L       H +  ++  L   P P   P        +
Sbjct: 121 ASAVYNEMEPK---HQLGHPSLML------SHKMCVDKSFLELKPPPFRAPYQLIHNHQQ 180

Query: 182 MVYLRSNNGINLNFDSSSCTQHTMS---STRSFISSLSIDGSVANLDGSAFHLIGAPRSS 241
           + Y RSN+G+NL FD S  + +T S    +RSF+SSLS+D SV + D ++FHL G  R S
Sbjct: 181 IAYSRSNSGVNLKFDGSGSSCYTPSVSNGSRSFVSSLSMDASVTDYDRNSFHLTGLSRGS 240

Query: 242 DQNSHHKRKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSW 301
           DQ  H ++ CS     GS+KCGS  +CHCSKKRK RVKRSIKVPAISNK+ADIPPD YSW
Sbjct: 241 DQ-QHTRKMCS-----GSLKCGSRSKCHCSKKRKLRVKRSIKVPAISNKIADIPPDEYSW 286

Query: 302 RKYGQKPIKGSPHPRGYYK 312
           RKYGQKPIKGSPHPR  YK
Sbjct: 301 RKYGQKPIKGSPHPRYIYK 286

BLAST of Lsi02G022330 vs. TAIR 10
Match: AT2G24570.1 (WRKY DNA-binding protein 17 )

HSP 1 Score: 160.2 bits (404), Expect = 3.0e-39
Identity = 85/159 (53.46%), Postives = 111/159 (69.81%), Query Frame = 0

Query: 198 TMSSTRSFISS-LSIDGSVANLDGSAFHLIGAPRSSDQNS--------HHKRKC----SG 257
           ++SS  SF+SS ++ DGSV+   GS+  L  AP     +S         ++++C      
Sbjct: 143 SVSSNSSFMSSAITGDGSVSK--GSSIFLAPAPAVPVTSSGKPPLSGLPYRKRCFEHDHS 202

Query: 258 RGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDNYSWRKYGQKPIKGSP 317
            G  G +    +G+CHC K RK+R+KR+++VPA+S K+ADIPPD YSWRKYGQKPIKGSP
Sbjct: 203 EGFSGKISGSGNGKCHCKKSRKNRMKRTVRVPAVSAKIADIPPDEYSWRKYGQKPIKGSP 262

Query: 318 HPRGYYKCSSMRGCPARKHVERCLEEPSMLIVTYEAERR 344
           HPRGYYKCS+ RGCPARKHVER L++ +MLIVTYE E R
Sbjct: 263 HPRGYYKCSTFRGCPARKHVERALDDSTMLIVTYEGEHR 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O043362.4e-8150.66Probable WRKY transcription factor 21 OS=Arabidopsis thaliana OX=3702 GN=WRKY21 ... [more]
Q93WU62.4e-7349.45Probable WRKY transcription factor 74 OS=Arabidopsis thaliana OX=3702 GN=WRKY74 ... [more]
Q9SR079.5e-7049.58Probable WRKY transcription factor 39 OS=Arabidopsis thaliana OX=3702 GN=WRKY39 ... [more]
Q32SG42.0e-5641.98Protein WRKY1 OS=Zea mays OX=4577 PE=1 SV=1[more]
Q9SJA84.3e-3853.46Probable WRKY transcription factor 17 OS=Arabidopsis thaliana OX=3702 GN=WRKY17 ... [more]
Match NameE-valueIdentityDescription
E7CEY52.4e-16989.91WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY51 PE=2 SV=1[more]
A0A5D3DSD05.4e-16990.23Putative WRKY transcription factor 21 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
N1K0K37.1e-16989.63WRKY transcription factor 21 OS=Cucumis sativus OX=3659 GN=WRKY-21 PE=2 SV=1[more]
A0A1S3BQJ02.1e-16889.94LOW QUALITY PROTEIN: probable WRKY transcription factor 21 OS=Cucumis melo OX=36... [more]
A0A2S0USY94.2e-16188.82WRKY transcription factor 21-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038899952.13.4e-17394.15probable WRKY transcription factor 74 isoform X1 [Benincasa hispida][more]
XP_004146948.25.0e-16989.91probable WRKY transcription factor 21 [Cucumis sativus] >ADU52528.1 WRKY protein... [more]
KAA0059712.11.1e-16890.23putative WRKY transcription factor 21 [Cucumis melo var. makuwa] >TYK26150.1 put... [more]
CCV01211.11.5e-16889.63WRKY transcription factor 21 [Cucumis sativus][more]
XP_008451288.14.2e-16889.94PREDICTED: LOW QUALITY PROTEIN: probable WRKY transcription factor 21 [Cucumis m... [more]
Match NameE-valueIdentityDescription
AT2G30590.11.7e-8250.66WRKY DNA-binding protein 21 [more]
AT5G28650.11.7e-7449.45WRKY DNA-binding protein 74 [more]
AT3G04670.16.7e-7149.58WRKY DNA-binding protein 39 [more]
AT3G04670.23.1e-5246.71WRKY DNA-binding protein 39 [more]
AT2G24570.13.0e-3953.46WRKY DNA-binding protein 17 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 286..346
e-value: 3.7E-30
score: 116.2
IPR003657WRKY domainPFAMPF03106WRKYcoord: 288..341
e-value: 2.7E-22
score: 78.8
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 281..347
score: 26.69305
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 265..339
e-value: 1.3E-28
score: 100.4
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 284..340
IPR018872Zn-cluster domainPFAMPF10533Plant_zn_clustcoord: 239..284
e-value: 3.9E-18
score: 65.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 233..247
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 227..250
NoneNo IPR availablePANTHERPTHR31282:SF163WRKY DNA-BINDING PROTEIN 21-RELATEDcoord: 3..344
IPR044810WRKY transcription factor, plantPANTHERPTHR31282WRKY TRANSCRIPTION FACTOR 21-RELATEDcoord: 3..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G022330.1Lsi02G022330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding