Lsi02G022250 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G022250 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.7e-71
Identity = 146/337 (43.32%), Postives = 204/337 (60.53%), Query Frame = 0

Query: 4   AKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNASKEF 63
           A FVSNW   +SV ES++ P +KR     VP+ N +P+IDL  H   LLV++I  A ++F
Sbjct: 2   ATFVSNW-STKSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQQHHH-LLVQQITKACQDF 61

Query: 64  GFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAK-----ECSK---DPNKSCRVYTSSE 123
           G FQVINHG  + +  ETM + KEF A+   EK K     E +K      +  ++Y   E
Sbjct: 62  GLFQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGE 121

Query: 124 NYMKEQTHCWRDALIFNCHPLEK-YVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSE 183
               E+   W+D L   CHPL++  V+ WP+ P +YREVV  Y V +RKL + +L+ + E
Sbjct: 122 QLSNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICE 181

Query: 184 GLGLGKGYFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQ--VL 243
           GLGL  GYF  E+S+  ++L N+YP CP+PS TLG   H D +LIT+L QD+ GLQ  ++
Sbjct: 182 GLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIV 241

Query: 244 KDGQWIGVQPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAI 303
           KD  WI VQPI  AFVVN+G  L++ITN K + + HR VT+    R S+  L+ P     
Sbjct: 242 KDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCT 301

Query: 304 MEPAKCLINETNPPRYRSLNFKDFQGNYLPRAGDTNS 330
           +EPAK L+N+ NPP Y+  ++ +F   YL    D +S
Sbjct: 302 IEPAKELLNQDNPPLYKPYSYSEFADIYLSDKSDYDS 335

BLAST of Lsi02G022250 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.4e-68
Identity = 143/340 (42.06%), Postives = 202/340 (59.41%), Query Frame = 0

Query: 4   AKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNASKEF 63
           AK +S      ++PE+YV P   RP    V      P+IDLS+ DR+ L+++I  A   F
Sbjct: 3   AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62

Query: 64  GFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQTH 123
           GFFQVINHGV+K++ +E + + +EF +MS  EK K  S DP K+ R+ TS  N  KE+ +
Sbjct: 63  GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122

Query: 124 CWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKGYF 183
            WRD L  +C+P+ KYV+ WP NPP ++E+V  Y   +R++  +I EL+SE LGL K Y 
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182

Query: 184 GGEMSE-NPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQD--VNGLQVLKDGQWIGV 243
              + E    + VN+YP CP P LT GL  H DP+ +TILLQD  V GLQ+L DGQW  V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242

Query: 244 QPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCL- 303
            P  +AFV+NIG  LQ ++NG  K+  HRAVTN +  R S+   + P D A+M PAK L 
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302

Query: 304 --INETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSN 338
              ++   P Y+   + ++   +  R  D    ++   +N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of Lsi02G022250 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.3e-65
Identity = 129/322 (40.06%), Postives = 187/322 (58.07%), Query Frame = 0

Query: 14  ESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNASKEFGFFQVINHGV 73
           +++P  YV P  +RP   +V  +  IPV+DL++ DR  +V  + +A +  GFFQV+NHG+
Sbjct: 18  DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77

Query: 74  SKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQTHCWRDALIFNC 133
              +    M + +EF  +   EKAK  S DP K  R+ TS  N  KE  H WRD L  +C
Sbjct: 78  DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHC 137

Query: 134 HPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKGYFGGEMSENPL- 193
           +PL ++V  WP NPP ++E++G YC  +R+L   + E +SE LGL  GY    + E    
Sbjct: 138 YPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 197

Query: 194 LLVNHYPRCPNPSLTLGLSQHCDPSLITILLQD--VNGLQVLKDGQWIGVQPIDNAFVVN 253
           + VN+YP+CP P LT GL  H DP+ +TILL D  V GLQVL DG+WI V P   A V+N
Sbjct: 198 MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVIN 257

Query: 254 IGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLINETNPPRYRS 313
           IG  LQ ++NGK ++  HRAV N+   R S+   + P +   + PAK LI + +P  YR+
Sbjct: 258 IGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRN 317

Query: 314 LNFKDFQGNYLPRAGDTNSVMQ 333
             + ++   +  R  D    ++
Sbjct: 318 YTYDEYYKKFWSRNLDQEHCLE 338

BLAST of Lsi02G022250 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.8e-60
Identity = 131/327 (40.06%), Postives = 190/327 (58.10%), Query Frame = 0

Query: 13  VESVPESYVYPPEKRPG-NVVVPMENAIPVIDLSTHD-----RTLLVRKILNASKEFGFF 72
           V+ VP +YV P   RP  + V    ++IP+IDL  HD     R  ++ +  +A    GFF
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAHACSSCGFF 74

Query: 73  QVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQTHCWR 132
           Q+ NHGV +   ++ M   +EF   S  E+ K  S D  K+ R+ TS  N  KE+   WR
Sbjct: 75  QIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWR 134

Query: 133 DALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKGYFGGE 192
           D L  +C+P+E +++ WP  P  +REV   Y   +R LVL +LE +SE LGL K      
Sbjct: 135 DFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNT 194

Query: 193 MSEN-PLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQD-VNGLQVLKDGQWIGVQPID 252
           + ++   + +N+YPRCP P LT GL  H D +LIT+LLQD V+GLQV KDG+WI V P+ 
Sbjct: 195 IGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVP 254

Query: 253 NAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLIN--E 312
           N F+VN+G  +Q+I+N K K+  HRAV N+   R S+     P ++A++ PA+ LIN  E
Sbjct: 255 NTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEE 314

Query: 313 TNPPRYRSLNFKDFQGNYLPRAGDTNS 330
            +P  YR+  + ++   +   A DT S
Sbjct: 315 DSPAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of Lsi02G022250 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 226.9 bits (577), Expect = 3.5e-58
Identity = 126/309 (40.78%), Postives = 183/309 (59.22%), Query Frame = 0

Query: 15  SVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNASKEFGFFQVINHGVS 74
           S+PES+V+  +K P      +  ++P+IDLS   R  + R IL A KE GFFQV+NHGVS
Sbjct: 13  SLPESFVFASDKVPPATKAVV--SLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNHGVS 72

Query: 75  KRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQTHCWRDALIFNC- 134
           K+V  +   + ++F  +   +KA   S++ +K  R++ S   Y       WRD L   C 
Sbjct: 73  KQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLF-SGATYDTGGEKYWRDCLRLACP 132

Query: 135 HPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKGYFGGEMS-ENPL 194
            P++  ++ WP  P   R+V+  +    R +  E+L L+ EG+G+   YF G++S  N +
Sbjct: 133 FPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGGNVI 192

Query: 195 LLVNHYPRCPNPSLTLGLSQHCDPSLITILLQD-VNGLQVLKDGQWIGVQPIDNAFVVNI 254
           L +NHYP CPNP   LG   HCD +LIT+LL   VNGL+V   G WI V P  NAFVVN 
Sbjct: 193 LNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFVVNF 252

Query: 255 GFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLINETNPPRYRSL 314
           G  L+++TNG LK+ EHRA+TN+  +R S+   + P  E ++ PAK  +++ NPP YR+ 
Sbjct: 253 GQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCYRTT 312

Query: 315 NFKDFQGNY 321
            F+DF   Y
Sbjct: 313 MFRDFMRIY 317

BLAST of Lsi02G022250 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 5.8e-181
Identity = 307/338 (90.83%), Postives = 321/338 (94.97%), Query Frame = 0

Query: 2   GDAKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNASK 61
           G AKFVSNW+ VESVPESYVYPPEKRPGN+VVPM  AIPVIDLS  DRTLLVRKIL+ASK
Sbjct: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63

Query: 62  EFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQ 121
           EFGFFQ+INHGVSK+VSEETMRIFKEFHAMSGPEKAKECSKDPN+SCRVYTSSENY KEQ
Sbjct: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123

Query: 122 THCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKG 181
            HCWRDALIFNCHPLEKYVHFWPQ PP+YREVVGAYC+ MRKLVLEILELMSEGLGLGKG
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIGVQ 241
           YFGGEMSENPLLLVNHYP CPNPSLTLGLSQHCDPSLITIL QDVNGLQVLKDGQWIGVQ
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLIN 301
           PIDNAFVVNIGFVLQIITNGKL+AAEHRAVTN+KTSRQSLTYLVYPKDEA+MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303

Query: 302 ETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQS 340
           E NPPRYRSLNFKDFQ NYLPRA DT +VMQYI +NQS
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Lsi02G022250 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.1e-178
Identity = 306/338 (90.53%), Postives = 315/338 (93.20%), Query Frame = 0

Query: 2   GDAKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNASK 61
           GDAKFVSNW+  ESVPESYVYPPEKRPGNVVVPM  AIPVIDLS  DR LLVRKIL ASK
Sbjct: 4   GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63

Query: 62  EFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQ 121
           E GFFQ+INHGVSKRVSE TMRIFKEFHAMSGPEKAKECSKDPN+SCRVYTSSENY KEQ
Sbjct: 64  ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123

Query: 122 THCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKG 181
            HCWRDALIFNCHPLEKYVHFWPQNPP+YREVVGAYCV MRKLVLEILELMSEGLGLGKG
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIGVQ 241
           Y GGEMSENPLLLVNHYP CPNPSLTLGLSQHCDPSLITIL QDVNGLQVLKDGQWIGVQ
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLIN 301
           PID AFVVNIGFVLQIITNGKL+AAEHRAVTN+KTSRQSLTYLVYPKDEA MEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303

Query: 302 ETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQS 340
           E NPPRYRSLNFKDFQ NYLPRA DT +VMQYI +NQS
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Lsi02G022250 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.8e-173
Identity = 289/338 (85.50%), Postives = 312/338 (92.31%), Query Frame = 0

Query: 1   MGDAKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNAS 60
           MG+ KFVSNW+GV+SVP  YVYPPEKRPGNVVVPM NAIPV+DLSTHDR LLVRKIL+AS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVS+RV EET RIFK+FHAMSG EK KECS+DPNKSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGK 180
           QTHCWRDALIFNCHPL+KYVHFWPQNPP+YREVVGAYC+ MR LVLEILEL+SEGLGLG+
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIGV 240
           GY GGEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITIL Q+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 QPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLI 300
            PIDNAFVVNIGFVLQ+I+NGKLKAAEHR VTNAK SRQSLTYLVYPKDE  MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQ 339
           NE NPPRYR+L FKDFQ NYLPRA DT +VM+ IGS++
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

BLAST of Lsi02G022250 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.4e-171
Identity = 290/339 (85.55%), Postives = 310/339 (91.45%), Query Frame = 0

Query: 1   MGDAKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNAS 60
           MG+ KFVSNW+GV+SVP  Y+YPPEKRPGNVVVPM NAIPV+DLST DR LLVRKIL+AS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVS+RV EET RIFK+FHAMSG EKAKECS+DP KSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGK 180
           QTHCWRDALIFNCHPLEKYVHFWPQNPP+YREVVGAYCV MR LVLEILEL+SEGLGLG+
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIGV 240
           GY   EMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITIL Q+VNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 QPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLI 300
            PIDNAFVVNIGFVLQ+I+NGKLKAAEHRAVTNAK SRQSLTYLVYPKDE  MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQS 340
           NE NPPRYR+L FKDFQ NYLPRA DT +VM+ IGSN S
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339

BLAST of Lsi02G022250 vs. ExPASy TrEMBL
Match: A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.4e-166
Identity = 278/340 (81.76%), Postives = 306/340 (90.00%), Query Frame = 0

Query: 1   MGD-AKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNA 60
           MGD   FVSNW GVESVPESYVYPPEKRPGNV VP+ENAIPV+DL+ HDR  LV KIL+A
Sbjct: 1   MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60

Query: 61  SKEFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMK 120
           S+EFGFFQVINHGVSK + EETMR+F+EFHAMSGPEK +ECSKDPN+SCRVYTSSEN++K
Sbjct: 61  SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120

Query: 121 EQTHCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLG 180
           E+THCWRDALIFNCHPL+KYVHFWPQNP  YREVVGAYC+VMRKLV+EILEL+SEGLGLG
Sbjct: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180

Query: 181 KGYFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIG 240
           KGYFG EMSENPLLLVNHYP CPNPSLTLGLSQHCDPSLITIL Q +NGLQVLKDGQWIG
Sbjct: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240

Query: 241 VQPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCL 300
           V P+DNAFVVNIGFVLQ+++NGKLKA EHRAV NAK SRQS+TYLVYPKD+A MEPAKC+
Sbjct: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300

Query: 301 INETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQS 340
           INE NP  Y SL FKDFQ NYLP A DT +V+QYI S+ S
Sbjct: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 340

BLAST of Lsi02G022250 vs. NCBI nr
Match: XP_038897058.1 (hyoscyamine 6-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 311/340 (91.47%), Postives = 323/340 (95.00%), Query Frame = 0

Query: 1   MGDA-KFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNA 60
           MGDA KFV+NW+GV+SVPESYVYPP KRPGNVVVPMENAIPVIDLS  DR LL+RKIL+A
Sbjct: 1   MGDANKFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDA 60

Query: 61  SKEFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMK 120
           SKEFGFFQVINHGVSK VS ETMRIFKEFHAMSGPEKAKECSKDPN+SCRVYTSSENYMK
Sbjct: 61  SKEFGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMK 120

Query: 121 EQTHCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLG 180
           EQ HCWRDALIFNCHPLEKYVHFWPQNPP+YREVVGAYCV MRKLVLEILELMSEGLGLG
Sbjct: 121 EQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLG 180

Query: 181 KGYFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIG 240
           KGYFGGEMSENPLLLVNHYP CPNPSLTLGLSQHCDPSLITIL QDVNGLQVLKDG+WIG
Sbjct: 181 KGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIG 240

Query: 241 VQPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCL 300
           VQPIDNAFVVNIGFVLQ+ITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEA MEPAKC+
Sbjct: 241 VQPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCM 300

Query: 301 INETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQS 340
           INE NP RYRSLNFKDFQ NYLPRA DT +VM+YIGSNQS
Sbjct: 301 INEANPRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS 340

BLAST of Lsi02G022250 vs. NCBI nr
Match: XP_004146985.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa_016058 [Cucumis sativus])

HSP 1 Score: 643.3 bits (1658), Expect = 1.2e-180
Identity = 307/338 (90.83%), Postives = 321/338 (94.97%), Query Frame = 0

Query: 2   GDAKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNASK 61
           G AKFVSNW+ VESVPESYVYPPEKRPGN+VVPM  AIPVIDLS  DRTLLVRKIL+ASK
Sbjct: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63

Query: 62  EFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQ 121
           EFGFFQ+INHGVSK+VSEETMRIFKEFHAMSGPEKAKECSKDPN+SCRVYTSSENY KEQ
Sbjct: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123

Query: 122 THCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKG 181
            HCWRDALIFNCHPLEKYVHFWPQ PP+YREVVGAYC+ MRKLVLEILELMSEGLGLGKG
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIGVQ 241
           YFGGEMSENPLLLVNHYP CPNPSLTLGLSQHCDPSLITIL QDVNGLQVLKDGQWIGVQ
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLIN 301
           PIDNAFVVNIGFVLQIITNGKL+AAEHRAVTN+KTSRQSLTYLVYPKDEA+MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303

Query: 302 ETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQS 340
           E NPPRYRSLNFKDFQ NYLPRA DT +VMQYI +NQS
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Lsi02G022250 vs. NCBI nr
Match: XP_008451261.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 634.8 bits (1636), Expect = 4.3e-178
Identity = 306/338 (90.53%), Postives = 315/338 (93.20%), Query Frame = 0

Query: 2   GDAKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNASK 61
           GDAKFVSNW+  ESVPESYVYPPEKRPGNVVVPM  AIPVIDLS  DR LLVRKIL ASK
Sbjct: 4   GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63

Query: 62  EFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQ 121
           E GFFQ+INHGVSKRVSE TMRIFKEFHAMSGPEKAKECSKDPN+SCRVYTSSENY KEQ
Sbjct: 64  ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123

Query: 122 THCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKG 181
            HCWRDALIFNCHPLEKYVHFWPQNPP+YREVVGAYCV MRKLVLEILELMSEGLGLGKG
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIGVQ 241
           Y GGEMSENPLLLVNHYP CPNPSLTLGLSQHCDPSLITIL QDVNGLQVLKDGQWIGVQ
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLIN 301
           PID AFVVNIGFVLQIITNGKL+AAEHRAVTN+KTSRQSLTYLVYPKDEA MEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303

Query: 302 ETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQS 340
           E NPPRYRSLNFKDFQ NYLPRA DT +VMQYI +NQS
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Lsi02G022250 vs. NCBI nr
Match: XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617.1 bits (1590), Expect = 9.2e-173
Identity = 292/339 (86.14%), Postives = 313/339 (92.33%), Query Frame = 0

Query: 1   MGDAKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNAS 60
           MG+ KFVSNW+GV+SVP  YVYPPEKRPGNVVVPM N IPV+DLST DR LLVRKIL+AS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVSKRV EET RIFK+FHAMSG EKAKECS+DPNKSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGK 180
           QTHCWRDALIFNCHPL+KYVHFWPQNPP+YREVVGAYCV MR LVLEILEL+SEGLGLG+
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180

Query: 181 GYFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIGV 240
           GY GGEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITIL Q+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 QPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLI 300
            PIDNAFVVNIGFVLQ+I+NGKLKAAEHRAVTNAK SRQSLTYLVYPKDE  MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQS 340
           NE NPPRYR+L FKDFQ NYLPRA DT +VM+ IGS++S
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339

BLAST of Lsi02G022250 vs. NCBI nr
Match: XP_022959666.1 (hyoscyamine 6-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 616.7 bits (1589), Expect = 1.2e-172
Identity = 289/338 (85.50%), Postives = 312/338 (92.31%), Query Frame = 0

Query: 1   MGDAKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNAS 60
           MG+ KFVSNW+GV+SVP  YVYPPEKRPGNVVVPM NAIPV+DLSTHDR LLVRKIL+AS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  KEFGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKE 120
           +EFGFFQVINHGVS+RV EET RIFK+FHAMSG EK KECS+DPNKSCRVYTSSENY KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGK 180
           QTHCWRDALIFNCHPL+KYVHFWPQNPP+YREVVGAYC+ MR LVLEILEL+SEGLGLG+
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLKDGQWIGV 240
           GY GGEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITIL Q+VNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 QPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLI 300
            PIDNAFVVNIGFVLQ+I+NGKLKAAEHR VTNAK SRQSLTYLVYPKDE  MEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSNQ 339
           NE NPPRYR+L FKDFQ NYLPRA DT +VM+ IGS++
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

BLAST of Lsi02G022250 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 4.5e-69
Identity = 143/340 (42.06%), Postives = 202/340 (59.41%), Query Frame = 0

Query: 4   AKFVSNWKGVESVPESYVYPPEKRPGNVVVPMENAIPVIDLSTHDRTLLVRKILNASKEF 63
           AK +S      ++PE+YV P   RP    V      P+IDLS+ DR+ L+++I  A   F
Sbjct: 3   AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62

Query: 64  GFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQTH 123
           GFFQVINHGV+K++ +E + + +EF +MS  EK K  S DP K+ R+ TS  N  KE+ +
Sbjct: 63  GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122

Query: 124 CWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKGYF 183
            WRD L  +C+P+ KYV+ WP NPP ++E+V  Y   +R++  +I EL+SE LGL K Y 
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182

Query: 184 GGEMSE-NPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQD--VNGLQVLKDGQWIGV 243
              + E    + VN+YP CP P LT GL  H DP+ +TILLQD  V GLQ+L DGQW  V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242

Query: 244 QPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCL- 303
            P  +AFV+NIG  LQ ++NG  K+  HRAVTN +  R S+   + P D A+M PAK L 
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302

Query: 304 --INETNPPRYRSLNFKDFQGNYLPRAGDTNSVMQYIGSN 338
              ++   P Y+   + ++   +  R  D    ++   +N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of Lsi02G022250 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.7e-61
Identity = 131/327 (40.06%), Postives = 190/327 (58.10%), Query Frame = 0

Query: 13  VESVPESYVYPPEKRPG-NVVVPMENAIPVIDLSTHD-----RTLLVRKILNASKEFGFF 72
           V+ VP +YV P   RP  + V    ++IP+IDL  HD     R  ++ +  +A    GFF
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL--HDLHGPNRADIINQFAHACSSCGFF 74

Query: 73  QVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQTHCWR 132
           Q+ NHGV +   ++ M   +EF   S  E+ K  S D  K+ R+ TS  N  KE+   WR
Sbjct: 75  QIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWR 134

Query: 133 DALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKGYFGGE 192
           D L  +C+P+E +++ WP  P  +REV   Y   +R LVL +LE +SE LGL K      
Sbjct: 135 DFLRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNT 194

Query: 193 MSEN-PLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQD-VNGLQVLKDGQWIGVQPID 252
           + ++   + +N+YPRCP P LT GL  H D +LIT+LLQD V+GLQV KDG+WI V P+ 
Sbjct: 195 IGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVP 254

Query: 253 NAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLIN--E 312
           N F+VN+G  +Q+I+N K K+  HRAV N+   R S+     P ++A++ PA+ LIN  E
Sbjct: 255 NTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEE 314

Query: 313 TNPPRYRSLNFKDFQGNYLPRAGDTNS 330
            +P  YR+  + ++   +   A DT S
Sbjct: 315 DSPAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of Lsi02G022250 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.4e-57
Identity = 123/332 (37.05%), Postives = 188/332 (56.63%), Query Frame = 0

Query: 7   VSNWKGVESVPESYVYPPEKRPG-NVVVPMENAIPVIDLST---HDRTLLVRKILNASKE 66
           VS++     +P +YV P   RP  + V    ++IP+IDL      +R ++V+++ +A   
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 67  FGFFQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQT 126
           +GFFQ+ NHGV      +   + +EF      E+ K  S DP K+ R+ TS  N   ++ 
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130

Query: 127 HCWRDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKGY 186
             WRD L  +C P+E ++  WP +P  +REV   Y   +R LVL +LE +SE LGL   +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190

Query: 187 FGGEMSENPL-LLVNHYPRCPNPSLTLGLSQHCDPSLITILLQD-VNGLQVLKDGQWIGV 246
               + ++   +  N+YP CP P LT GL  H DP++IT+LLQD V+GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250

Query: 247 QPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLI 306
            PI N F+VNIG  +Q+I+N K K+  HRAV N +  R S+    +P  +A++ PA  L+
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310

Query: 307 NETNP-PRYRSLNFKDFQGNYLPRAGDTNSVM 332
           NE +    YR+  F ++   +  R+  T S +
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKFWNRSLATASCL 341

BLAST of Lsi02G022250 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 205.3 bits (521), Expect = 7.8e-53
Identity = 119/318 (37.42%), Postives = 173/318 (54.40%), Query Frame = 0

Query: 12  GVESVPESYVYPPEKRP--GNVVVPMENAIPVIDLS-THD---RTLLVRKILNASKEFGF 71
           GV  VP+ YV PP +RP  G+ +   E  +PVIDLS  H    R+L + +I  A KEFGF
Sbjct: 23  GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82

Query: 72  FQVINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYMKEQTHCW 131
           FQVINHG+   V  + +    +F  +   EK    S + ++  R Y +S N+  ++ H W
Sbjct: 83  FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYW 142

Query: 132 RDALIFNCHPLEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLGLGKGYFGG 191
           RD +    HPL K++  WP NPP Y++ VG Y      L  +++E +SE LGL K Y   
Sbjct: 143 RDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE 202

Query: 192 EMSE-NPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQDVNGLQVLK-DGQWIGVQPI 251
           E+ E + ++ VN YP CP P + LG+  H D S +TILLQ   GLQ++  +  W+ V  I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYI 262

Query: 252 DNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIMEPAKCLINET 311
           + A +V +G  +++++NG  K+  HR   N +  R S   L        + PA  L+N  
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPN 322

Query: 312 NPPRYRSLNFKDFQGNYL 322
           N P Y   +F DF  NY+
Sbjct: 323 NAPAYGEFSFNDFL-NYI 338

BLAST of Lsi02G022250 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.5e-48
Identity = 119/323 (36.84%), Postives = 169/323 (52.32%), Query Frame = 0

Query: 18  ESYVYPPEKRPGNVVVP-----MENAIPVIDLST-----HDRTLLVRKILNASKEFGFFQ 77
           E+++  PE RP   +         + IP IDLS+     HD+T + ++I  A K +GFFQ
Sbjct: 6   EAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQ 65

Query: 78  VINHGVSKRVSEETMRIFKEFHAMSGPEKAKECSKDPNK---SCRVYTSSENYMKEQTHC 137
           VINHG+   +     +   EF  ++  EK K    + N        +T +    KE    
Sbjct: 66  VINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDF 125

Query: 138 W-RDALIFNCHP------LEKYVHFWPQNPPEYREVVGAYCVVMRKLVLEILELMSEGLG 197
           + +D+ I    P      L K  + WPQNP  +REV   Y   + KL   +LEL+S  LG
Sbjct: 126 FLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLG 185

Query: 198 L-GKGYFGGEMSENPLLLVNHYPRCPNPSLTLGLSQHCDPSLITILLQD-VNGLQVLK-- 257
           L G    G    +   L  NHYP CPNP L LG+ +H D   +T+L QD V GLQV +  
Sbjct: 186 LPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRS 245

Query: 258 DGQWIGVQPIDNAFVVNIGFVLQIITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEAIM 317
           DGQWI V+PI +A ++N+G  +Q+ TN +  +AEHR V N    R S+ +  +P  EA +
Sbjct: 246 DGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANI 305