Lsi02G022230 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G022230
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Locationchr02: 28649432 .. 28653406 (-)
RNA-Seq ExpressionLsi02G022230
SyntenyLsi02G022230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTAAAATTAATGTACTAGCTTTAGCATATGGTAATAAATAATGGAGAGGCTACCCAAAAATTAATGTACTAGCTTTAGCATATGGTAATAAATAATGGAGAGGCTGCCCAACCACTTTTAGCAGGTTATAACTTATAAGTAAGGGCGTACTGTGTTGGTGTGTGCCAGTTCATAAATGTTCAACTGAAAATGGTTAAAGATTTTGGGACTATAAATTATTATTATTATTATTATTATTATTATATTTATTTTTTTTACCTTGTAATTTGTCTACTTCACAAGTATATATATAAATACAAATATGCCATAGATTAAGTCACAACTATAAGAAAAGAAATAATGGAAGAAGCATCCAAAGAAGAGCCAAAGGAGATGGTGATGAAATCTCCTCCAAGGCTTTTTAAGAGGAAAAAGTGGACAAAGTTTGATAGAAATGTTGCCTTTTACTTTCTCCTTTTGCATCTTCTTTGTATTTTGGCACCCTTCTATTTTAGTTGGACTTGTTTCTTGCTTGCTTTGGGATTAGCCAATCTAACCGGTTTTGCCATTACTGTTTCGTATCATCGAAATCTTGCACATCGAAGTTTCAAACTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATCGCAGCTCATGCGATTCAGGTTCAATATTTCAAATCCCTCATCCTAACTAAAAATAGACTCAGCTATGAATATATAAGATTGTACATTATTCTTGTTTTTAATGATCATCATTTATAATCATGTATGCAAATCATAAAAGAAGAGTAGTGTAGTGTAATTGCCTACATGCATGTCATTCAAAAAATAAAACTAGTGATAAACTCTAGAGTATATTTTTTAAAATGTGTGTTTTGGTAACATTTTGTTGGTTTTTTAAATTTTAGGGTGATCCAATTGATTGGGTAAGCACACACAGATGTCATCATCGATTCGTTGATTCGGAACGAGACCCACATAGTCCTATATACGGATTTTGGTTTAGTCAAATGATGTGGTTCTTCGATTCGTATAATTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTTTGAAAAGGTGGAAAGAAATATGTTTATGTTTTACATGAAACATGGATATCCAGACAATGTGAGAGATTTGGAGAAACAAAGGTTCTATCGGTTTATACATAAAACATATTGGCTTCATCCAATTGCTCTTTCAGTGTTGCTCTTTGCTATTGGAGGAGTTCCTTTCATCATTTGGGGAATGGTAAAATTATATTATTATTCCAATTTCTTTTCCTCTTGTAATTATTAAGTTATGAATTCATATTATCTATGAATATGCCAATGTGTATGTAAATACATATGCTATTGCCAAGTATATTATTATTCCTATATAAACTTTTAAAAATTAATTACTTAATATTGCATCTTTTATGAATTCTATTTTTTTTTTAATTTTTACATAAGGATAAAGTTTTTACCATTTTTCTCTAATGTTAATTAAATTTCTCCATATTTAGGCAAGGCCATATACATAATAGCAAGTCTAAGGTTATTAAAAAAAAAGAGAGGAAATTTCATGCATGTATTGCTTTGTATTGACTTTGATAAAGGTAATTGTTGTTTTTTTTTTTAATTTAATTTTATTTTACAATTAATTGTTGATTTGTGTTATCTATAAGATTAAGTTACAAGTTTAATCTTTGAACTTTGAAATTTTAGTCAATTTAGAAATTTTAGTCAATTTAGTTCTTGAACTCTTACAAATGTATAGTAAGTCTATAAATTATTTGTGTCTGATAGATTCCTGCAAAATTATGATTTTTTTTCTAAAATGATTTATCTTTAAATAGAAATTTAAATTATATGTTTAACAGAGTCTTAAGACTTTCAATTTTTTTTCTAGAAGATTCTTGAAGTTAAAAAAGAATTCAAAAGTATAGAATGATTTCAATTTAGTTTTGTGTGAATTTTATATTTGTGTCCTCTCAATTTTCTTACCACGATTTTCATCCTTTTTAAAAGAAGCATTATATTTCTTAGTCAAACTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAACTACTTTTCTATTGGTAGAGAGTGAATCACATAACCAAAAAAAAAAATAATAATTATATGTGAGAATAACAATATTTATAAGCTTAATTTTAAAAAATAAAAATAGAACTAATTAAAAACTAAATAGTTTTCAAATGGAACCTGTTAAACTAATTTTGTAATTTTAAAAATTTAAAGACTAAATTGACACAAATTTCAAAGTTAAATAACTCAATCTGTTGGTAACCAATCTAATAAGTATTGTTTGATAATGATTATAAGTATTAATTAGGATTGTTTTCAAAAATAGAAAAATGAGTTGAACTATTTACAAATATAGAAAAATTTCACTGTCTGTCAGCGATAGACCGTTGACTTTTATCACTCAAGCGATAGAAGTCTATTATGATCTATCGCTGATAGATAGTAAAACTTTTCTATATTTAATTTGTAAATAATTTGATATGTTTTATATTTATAGTAATTTCTCTATTGATTAGTATAAATTAGAAATAAACACCCATTGAAGTGTGTGTTTTTAGTGGTTATTTTTGTTTCTTTAAATAATATTTCTACCCTCTAATTATTCTAGATTTTTTTTTGAAAGAAGTAATTCTAGATTTATTGTTAAGTATGACTATGAAATACTAATTAATCCTATTTTTATTTAATTAATTTCATTGAAATATTTTACTTACGAAAGTGTTTTATGTTTATTAAACTATTGATAGTGCGTGAGGTTCGTACTCGCTCTGCAAATCACATCTATGGTAAATTCAGTATGTCATATTTGGGGAAAGCAACCATGGAACACTGGTGATTTATCTAAGAACAATTGGTAAGAGTTATTTATTTTTTATTTTTTATTTTTTTTCCTCCTAAATAATTGGTTTTACATGGATGATTGATATATAAATTTTATGGTACTTATATCAATCCAATTGTTATAAATTTTTAAATATTCTATATCAATCTTTTACACTCCGTTTGGTAATGATTTTGTTTATATCAATTATTTTAAAGTACACAGCAAAATTTAATAACAATTACAAAAATGATGTTTACGTAAAATTTAGAGATCAAAGATCTATTTCAAATACCTTTAGAAAAAAAAAATTCCATACAAATGTTATTATATCTTACTTTTTTTTTTTTCAAAATTATTTTGGATACAAATTTATTTCGTTACACTAAACTCAAAAGTGATTATTTTATCGATTATGAAACATGTTTTTATTTCTTACCACATATTTTTAGGATTTTCAAATAATACCTTTAAAGTTATTTTTGAAGAATCTATCTCAAAATGTTTTTTTCTTAAAAAAAAAAAAAACAAATGTTAAATTAACTCTCTTTTTTTTTTAACTATTTCTTATTTATTAGGATGAAAAAAAAAACGTTTTTGATCATATAAAATATTCAATAAGTCATATCAAACCTACTTTAAATTATTAATTTTTCAAATGTACGTGCTTATAGAAAAATTAATTTTATTATTTAAAAAATATTTTTTCCAAACTATTCTAAACTGACCCTAAGTAAATACGACTAATTAACAATGTTTAAATAGTTCAATTAATTTCTTTTTTAGGATGGTAGCTTTATTAACTTTTGGAGAAGGATGGCACAATAACCACCATGCTTTCGAGTATTCAGCAAGACATGGTCATGAATGGTGGCAGATTGATTATGGTTGGTATTTTATCAAGTTTCTTCAAGTCATTGGAGTAGCTACCAATGTGAAATTACCTTCTGAAAAGCAAAAGTTGGCTGCTTTAAACAAATCAAAACCCACCTTTCTCAAGCCTATGATTAATTGA

mRNA sequence

ATGGGAAAAGAAATAATGGAAGAAGCATCCAAAGAAGAGCCAAAGGAGATGGTGATGAAATCTCCTCCAAGGCTTTTTAAGAGGAAAAAGTGGACAAAGTTTGATAGAAATGTTGCCTTTTACTTTCTCCTTTTGCATCTTCTTTGTATTTTGGCACCCTTCTATTTTAGTTGGACTTGTTTCTTGCTTGCTTTGGGATTAGCCAATCTAACCGGTTTTGCCATTACTGTTTCGTATCATCGAAATCTTGCACATCGAAGTTTCAAACTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATCGCAGCTCATGCGATTCAGGGTGATCCAATTGATTGGGTAAGCACACACAGATGTCATCATCGATTCGTTGATTCGGAACGAGACCCACATAGTCCTATATACGGATTTTGGTTTAGTCAAATGATGTGGTTCTTCGATTCGTATAATTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTTTGAAAAGGTGGAAAGAAATATGTTTATGTTTTACATGAAACATGGATATCCAGACAATGTGAGAGATTTGGAGAAACAAAGGTTCTATCGGTTTATACATAAAACATATTGGCTTCATCCAATTGCTCTTTCAGTGTTGCTCTTTGCTATTGGAGGAGTTCCTTTCATCATTTGGGGAATGTGCGTGAGGTTCGTACTCGCTCTGCAAATCACATCTATGGTAAATTCAGTATGTCATATTTGGGGAAAGCAACCATGGAACACTGGATGGCACAATAACCACCATGCTTTCGAGTATTCAGCAAGACATGGTCATGAATGGTGGCAGATTGATTATGGTTGGTATTTTATCAAGTTTCTTCAAGTCATTGGAGTAGCTACCAATGTGAAATTACCTTCTGAAAAGCAAAAGTTGGCTGCTTTAAACAAATCAAAACCCACCTTTCTCAAGCCTATGATTAATTGA

Coding sequence (CDS)

ATGGGAAAAGAAATAATGGAAGAAGCATCCAAAGAAGAGCCAAAGGAGATGGTGATGAAATCTCCTCCAAGGCTTTTTAAGAGGAAAAAGTGGACAAAGTTTGATAGAAATGTTGCCTTTTACTTTCTCCTTTTGCATCTTCTTTGTATTTTGGCACCCTTCTATTTTAGTTGGACTTGTTTCTTGCTTGCTTTGGGATTAGCCAATCTAACCGGTTTTGCCATTACTGTTTCGTATCATCGAAATCTTGCACATCGAAGTTTCAAACTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATCGCAGCTCATGCGATTCAGGGTGATCCAATTGATTGGGTAAGCACACACAGATGTCATCATCGATTCGTTGATTCGGAACGAGACCCACATAGTCCTATATACGGATTTTGGTTTAGTCAAATGATGTGGTTCTTCGATTCGTATAATTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTTTGAAAAGGTGGAAAGAAATATGTTTATGTTTTACATGAAACATGGATATCCAGACAATGTGAGAGATTTGGAGAAACAAAGGTTCTATCGGTTTATACATAAAACATATTGGCTTCATCCAATTGCTCTTTCAGTGTTGCTCTTTGCTATTGGAGGAGTTCCTTTCATCATTTGGGGAATGTGCGTGAGGTTCGTACTCGCTCTGCAAATCACATCTATGGTAAATTCAGTATGTCATATTTGGGGAAAGCAACCATGGAACACTGGATGGCACAATAACCACCATGCTTTCGAGTATTCAGCAAGACATGGTCATGAATGGTGGCAGATTGATTATGGTTGGTATTTTATCAAGTTTCTTCAAGTCATTGGAGTAGCTACCAATGTGAAATTACCTTCTGAAAAGCAAAAGTTGGCTGCTTTAAACAAATCAAAACCCACCTTTCTCAAGCCTATGATTAATTGA

Protein sequence

MGKEIMEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNTGWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEKQKLAALNKSKPTFLKPMIN
Homology
[Close]
BLAST of Lsi02G022230 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 320.9 bits (821), Expect = 1.7e-86
Identity = 158/302 (52.32%), Postives = 196/302 (64.90%), Query Frame = 0 

Query: 17  MVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTG-FAI 76
           +V K    ++  ++W   D       L +HLL +LAPF F+W    +A GL  +TG   I
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 77  TVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 136
           T+S+HRNL+H++FKLPKWLEY  AY  A A+QG+PIDWVSTHR HH+F DS+RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 137 GFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFI 196
           GFWFS M W FD+  +T++                       G P+NV DLEKQ FYRF+
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 197 HKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNT---- 256
             TY LHP+AL+V L+A+GG PFI+WGM VR V    IT +VNS CH+WGKQ WNT    
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 257 -------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEK 301
                        GWHNNHHAFE+SARHG EWWQ+D  WY +KFLQ IG+AT+VKLPSE 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 363

[Close]
BLAST of Lsi02G022230 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 285.4 bits (729), Expect = 7.8e-76
Identity = 141/301 (46.84%), Postives = 181/301 (60.13%), Query Frame = 0 

Query: 16  EMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTGFAI 75
           E+V+    R F  + WT +D +    F+  HLL +LAPFYFSW  F +   L  + G  I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 76  TVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 135
           T+SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  +++RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 136 GFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFI 195
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRF+
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 196 HKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNT---- 255
            +T  LH +A S L +  GG+P ++WG+ +   + L +T +VNSVCHIWG + WNT    
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 256 -------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEK 300
                        GWHNNHHAFE+SARHG EWWQ+D  W  I+FL+ IG+ATNVKLP+E 
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

[Close]
BLAST of Lsi02G022230 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 6.6e-75
Identity = 141/311 (45.34%), Postives = 181/311 (58.20%), Query Frame = 0 

Query: 7   EEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALG 66
           E   K    +  M    R    +KW + D   AF  L +H LC+LAPF F+W    +AL 
Sbjct: 10  ENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALI 69

Query: 67  LANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 126
           +  + G  ITVSYHRNLAHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS
Sbjct: 70  VYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDS 129

Query: 127 ERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDL 186
           +RDPHSP  GFWFS ++W FD+  L  K                       G   NV DL
Sbjct: 130 DRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNVEDL 189

Query: 187 EKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGK 246
           ++Q +Y+F+ +T   H +    LL+  GG+ F+ WGM +   +   +T ++NS+CH+WG 
Sbjct: 190 KRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGS 249

Query: 247 QPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVA 301
           + W T                  WHNNHHAFE SAR G EWWQID  WY ++FL++IG+A
Sbjct: 250 RTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLA 298

[Close]
BLAST of Lsi02G022230 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 282.0 bits (720), Expect = 8.6e-75
Identity = 139/293 (47.44%), Postives = 176/293 (60.07%), Query Frame = 0 

Query: 25  LFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTGFAITVSYHRNLA 84
           +F  ++W + D         +H L +LAPFYF+W+   +      + G  ITVSYHRNLA
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 89

Query: 85  HRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMMW 144
           HRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DSERDPHSP  GFWFS ++W
Sbjct: 90  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 149

Query: 145 FFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFIHKTYWLHPI 204
            +DS  L  K                       G   NV DL++Q FYRF+ KT   H +
Sbjct: 150 IYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHIL 209

Query: 205 ALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNT------------- 264
            L   LF +GG+ F+ WGM V   L + +T ++NS+CHIWG + W T             
Sbjct: 210 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 269

Query: 265 ----GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEKQK 301
                WHNNHHAFE SAR G EWWQID  WY ++F ++IG+AT+VK+P+E Q+
Sbjct: 270 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQR 300

[Close]
BLAST of Lsi02G022230 vs. ExPASy Swiss-Prot
Match: Q9LND8 (Delta-9 desaturase-like 2 protein OS=Arabidopsis thaliana OX=3702 GN=At1g06100 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.8e-72
Identity = 145/314 (46.18%), Postives = 187/314 (59.55%), Query Frame = 0 

Query: 6   MEEASKEE--PKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLL 65
           M E +K++   ++  ++   R +  +KWT+FD   A     +HLLC+LAPF + W  F  
Sbjct: 1   MSETTKDDGSSQKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRF 60

Query: 66  ALGLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRF 125
            + LA LT   IT SYHRNL HRSFKLPKWLEY  AY A  A+QGDP+DWVS HR HH+F
Sbjct: 61  GIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQF 120

Query: 126 VDSERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNV 185
            DS+RDPHSPI GFWFS ++W FD+ +  R+ C                     G  +NV
Sbjct: 121 TDSDRDPHSPIEGFWFSHVLWIFDT-DYIREKC---------------------GRRNNV 180

Query: 186 RDLEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHI 245
            DL++Q FYRF+ KT  LH +A   L++  GG+P++ W +    V+    T +VNS CHI
Sbjct: 181 MDLKQQWFYRFLKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHI 240

Query: 246 WGKQPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVI 301
            G Q W T                  WHNNHHAFE SARHG EW+Q+D  WY I F Q +
Sbjct: 241 CGSQAWQTNDTSRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQAL 292

[Close]
BLAST of Lsi02G022230 vs. ExPASy TrEMBL
Match: A0A1S3BS49 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492608 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.3e-168
Identity = 283/329 (86.02%), Postives = 295/329 (89.67%), Query Frame = 0 

Query: 6   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 65
           MEEASKEE KEMVM SP RLFKRKKWTKFD+NVAFYFLLLHLLCILAPFYFSWTC  L+L
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSL 60

Query: 66  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 125
           GLANLTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120

Query: 126 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 185
           SERDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNNF+KVERN FMFYMKHGY DNV D
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTD 180

Query: 186 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 245
           LEKQRFYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV++L  TSM+NSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWG 240

Query: 246 KQPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 305
            QPWNT                 GWHNNHHAFEYSARHGHEWWQIDYGWY IKFLQVIG 
Sbjct: 241 NQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGF 300

Query: 306 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 318
           ATNVKLPSEKQK AALNKSKPTFLKPMI+
Sbjct: 301 ATNVKLPSEKQKWAALNKSKPTFLKPMID 329

[Close]
BLAST of Lsi02G022230 vs. ExPASy TrEMBL
Match: A0A0A0KAB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 3.8e-166
Identity = 278/329 (84.50%), Postives = 296/329 (89.97%), Query Frame = 0 

Query: 6   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 65
           M+E SKE+PKEMVMKSPPRLF+RKKWTKFDRNVAFYFL LHLLCILAPFYFSWTC LLAL
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 66  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 125
           GL+ LTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 126 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 185
           S RDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNN++KVERN+FMFYMKHGYPDNV D
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180

Query: 186 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 245
           LEKQ+FYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV +L +TSMVNSVCHIWG
Sbjct: 181 LEKQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 246 KQPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 305
            QPWNT                 GWHNNHHAFEYSARHGHEWWQIDYGWY I+FLQVIG+
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 306 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 318
           ATNVKLPSEKQKLAALNKSKP FLK MI+
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

[Close]
BLAST of Lsi02G022230 vs. ExPASy TrEMBL
Match: A0A1S3BQG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492609 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.5e-157
Identity = 265/328 (80.79%), Postives = 287/328 (87.50%), Query Frame = 0 

Query: 8   EASKEEPKEMV-MKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALG 67
           E SKE+PKE++ MKSPP+LF+RKKWTKFDRN AFYFL  HLLCILAPF FSWT  LLALG
Sbjct: 4   EVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLALG 63

Query: 68  LANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 127
           L+ L+G  I+VSYHRNLAHRSFKLPKW EYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS
Sbjct: 64  LSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 123

Query: 128 ERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDL 187
           ERDPHSPIYGFWFSQM W FDSYNLTRKVCPNYFNN++K ERN+FM YMKHGYPDNV DL
Sbjct: 124 ERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPDL 183

Query: 188 EKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGK 247
           EKQ FYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV ++ +TSMVNSVCH+WG 
Sbjct: 184 EKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWGN 243

Query: 248 QPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVA 307
           QPWNT                 GWHNNHHAFEYSARHG EWWQIDYGWYFI+FL+VIG+A
Sbjct: 244 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGLA 303

Query: 308 TNVKLPSEKQKLAALNKSKPTFLKPMIN 318
           TNVK PSEKQKLAALNKSKPTFLKP IN
Sbjct: 304 TNVKFPSEKQKLAALNKSKPTFLKPTIN 331

[Close]
BLAST of Lsi02G022230 vs. ExPASy TrEMBL
Match: A0A6J1GPG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456275 PE=3 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 9.0e-152
Identity = 257/319 (80.56%), Postives = 279/319 (87.46%), Query Frame = 0 

Query: 8   EASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGL 67
           EASK+ P   +++SPP LF RKKWTKFDRNVA YFLLLHLLCILAPFYF+WT F L + L
Sbjct: 2   EASKQHPN--MVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTSFFLTIIL 61

Query: 68  ANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 127
           ANLTG AI+VSYHRNLAHR+FKLPKWLEYSLAY AAHAIQGDPIDWVSTHRCHHR+VD+E
Sbjct: 62  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 121

Query: 128 RDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLE 187
           RDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYF NFEKVERNMFMF+MKHGYPDNVRDLE
Sbjct: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 181

Query: 188 KQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQ 247
           KQ FYRF+ KTYWLHP+AL+ LL+A+GGVP++IWGMCVRFVL+L ITSMVNSVCHIWG Q
Sbjct: 182 KQWFYRFMRKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVLSLHITSMVNSVCHIWGTQ 241

Query: 248 PWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVAT 307
           PWNT                 GWHNNHHAFEYSARHGHEWW+IDYGWY IKFLQVIG+AT
Sbjct: 242 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWEIDYGWYVIKFLQVIGLAT 301

Query: 308 NVKLPSEKQKLAALNKSKP 310
            VKLPS+ QKLAALNKSKP
Sbjct: 302 CVKLPSQTQKLAALNKSKP 318

[Close]
BLAST of Lsi02G022230 vs. ExPASy TrEMBL
Match: A0A6J1JUL6 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488510 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.2e-151
Identity = 255/319 (79.94%), Postives = 277/319 (86.83%), Query Frame = 0 

Query: 8   EASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGL 67
           EASK+ P   +++SPP LF RKKWTKFDRNVA YFLLLHLLCILAPFYF+WTCF L + L
Sbjct: 2   EASKQHPN--MVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIIL 61

Query: 68  ANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 127
           ANLTG AI+VSYHRNLAHR+FKLPKWLEYSLAY AAHAIQGDPIDWVSTHRCHHR+VD+E
Sbjct: 62  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 121

Query: 128 RDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLE 187
           RDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYF NFEKVERNMFMF+MKHGYPDNVRDLE
Sbjct: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 181

Query: 188 KQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQ 247
           KQ FYRF+ KTYWLHP+AL+ LL+A GG+P++IWGMCVRFVL+L +TSMVNSVCHIWG Q
Sbjct: 182 KQWFYRFMRKTYWLHPLALAGLLYAAGGIPYVIWGMCVRFVLSLHVTSMVNSVCHIWGNQ 241

Query: 248 PWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVAT 307
           PWNT                 GWHNNHHAFEYSARHGHEWWQIDYGWY IKFLQVIG+AT
Sbjct: 242 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 301

Query: 308 NVKLPSEKQKLAALNKSKP 310
            VKLPS+   LAALNKSKP
Sbjct: 302 CVKLPSQTHNLAALNKSKP 318

[Close]
BLAST of Lsi02G022230 vs. NCBI nr
Match: XP_038899717.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 606.7 bits (1563), Expect = 1.2e-169
Identity = 285/329 (86.63%), Postives = 299/329 (90.88%), Query Frame = 0 

Query: 6   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 65
           MEEASKE PKEMVMKSPPRLF+RKKWTKFDRNVAFYFL+LHLLCILAPFYFSWTC  LAL
Sbjct: 1   MEEASKEGPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLILHLLCILAPFYFSWTCLFLAL 60

Query: 66  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 125
           GL+ LTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHR+VD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRYVD 120

Query: 126 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 185
           SERDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNNF+KVERN FMFYMKHG+PDNV D
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGHPDNVTD 180

Query: 186 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 245
           LEKQRFYRFIHKTYWLHP+ALSVLL+AIGGVPFIIWGMCVRFV +L +TSMVNSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSVLLYAIGGVPFIIWGMCVRFVFSLHVTSMVNSVCHIWG 240

Query: 246 KQPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 305
           KQPWNT                 GWHNNHHAFEYSARHGHEWWQID+GWY IKFLQVIG+
Sbjct: 241 KQPWNTGDLSKNNWMVAILTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGL 300

Query: 306 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 318
           ATNVKLPSEKQKLAALNKSKPTFLKPMI+
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPTFLKPMID 329

[Close]
BLAST of Lsi02G022230 vs. NCBI nr
Match: XP_008451258.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 600.1 bits (1546), Expect = 1.1e-167
Identity = 283/329 (86.02%), Postives = 295/329 (89.67%), Query Frame = 0 

Query: 6   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 65
           MEEASKEE KEMVM SP RLFKRKKWTKFD+NVAFYFLLLHLLCILAPFYFSWTC  L+L
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSL 60

Query: 66  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 125
           GLANLTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120

Query: 126 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 185
           SERDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNNF+KVERN FMFYMKHGY DNV D
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTD 180

Query: 186 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 245
           LEKQRFYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV++L  TSM+NSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWG 240

Query: 246 KQPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 305
            QPWNT                 GWHNNHHAFEYSARHGHEWWQIDYGWY IKFLQVIG 
Sbjct: 241 NQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGF 300

Query: 306 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 318
           ATNVKLPSEKQK AALNKSKPTFLKPMI+
Sbjct: 301 ATNVKLPSEKQKWAALNKSKPTFLKPMID 329

[Close]
BLAST of Lsi02G022230 vs. NCBI nr
Match: XP_011659261.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KAE8646293.1 hypothetical protein Csa_016268 [Cucumis sativus])

HSP 1 Score: 591.3 bits (1523), Expect = 5.1e-165
Identity = 277/329 (84.19%), Postives = 295/329 (89.67%), Query Frame = 0 

Query: 6   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLAL 65
           M+E SKE+PKEMVMKSPPRLF+RKKWTKFDRNVAFYFL LHLLCILAPFYFSWTC LLAL
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 66  GLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 125
           GL+ LTG  I+VSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 126 SERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRD 185
           S RDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNN++KVERN+ MFYMKHGYPDNV D
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180

Query: 186 LEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWG 245
           LEKQRFYRF+HKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV +L +TSMVNSVCHIWG
Sbjct: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 246 KQPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGV 305
            QPWNT                 GWHNNHHAFEYSARHGHEWWQIDYGWY I+FLQVIG+
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 306 ATNVKLPSEKQKLAALNKSKPTFLKPMIN 318
           ATNVKLPSEKQKLAALNKSKP FLK MI+
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

[Close]
BLAST of Lsi02G022230 vs. NCBI nr
Match: XP_011659770.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus])

HSP 1 Score: 589.0 bits (1517), Expect = 2.5e-164
Identity = 278/330 (84.24%), Postives = 298/330 (90.30%), Query Frame = 0 

Query: 6   MEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAP-FYFSWTCFLLA 65
           ME ASKE+PKEMVM SP RLFKRKKWTKFD+NVAFYFLLLHLL ILAP FYFSW C LL+
Sbjct: 1   MEGASKEDPKEMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60

Query: 66  LGLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 125
           LGLANLTG AI+VSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV
Sbjct: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120

Query: 126 DSERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVR 185
           DSERDPHSPIYGFWFSQM WFFDSYNLTRKVCPNYFNN++KVERN+FMFYMKHGYPDNV 
Sbjct: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT 180

Query: 186 DLEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIW 245
           DLEKQ+FYRFIHKTYWLHP+ALS+LL+A+GG+PFIIWG+CVRFVL+L  TSM+NSVCHIW
Sbjct: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240

Query: 246 GKQPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIG 305
           G QPWNT                 GWHNNHHAFEYSARHGHEWWQID GWY IKFLQVIG
Sbjct: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300

Query: 306 VATNVKLPSEKQKLAALNKSKPTFLKPMIN 318
           +ATNVKLPSEKQK+AALNKSKPTFL+PMI+
Sbjct: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 330

[Close]
BLAST of Lsi02G022230 vs. NCBI nr
Match: XP_008451259.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 563.5 bits (1451), Expect = 1.1e-156
Identity = 265/328 (80.79%), Postives = 287/328 (87.50%), Query Frame = 0 

Query: 8   EASKEEPKEMV-MKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALG 67
           E SKE+PKE++ MKSPP+LF+RKKWTKFDRN AFYFL  HLLCILAPF FSWT  LLALG
Sbjct: 4   EVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLALG 63

Query: 68  LANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 127
           L+ L+G  I+VSYHRNLAHRSFKLPKW EYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS
Sbjct: 64  LSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 123

Query: 128 ERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDL 187
           ERDPHSPIYGFWFSQM W FDSYNLTRKVCPNYFNN++K ERN+FM YMKHGYPDNV DL
Sbjct: 124 ERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPDL 183

Query: 188 EKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGK 247
           EKQ FYRFIHKTYWLHP+ALS+LL+AIGG+PFIIWGMCVRFV ++ +TSMVNSVCH+WG 
Sbjct: 184 EKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWGN 243

Query: 248 QPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVA 307
           QPWNT                 GWHNNHHAFEYSARHG EWWQIDYGWYFI+FL+VIG+A
Sbjct: 244 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGLA 303

Query: 308 TNVKLPSEKQKLAALNKSKPTFLKPMIN 318
           TNVK PSEKQKLAALNKSKPTFLKP IN
Sbjct: 304 TNVKFPSEKQKLAALNKSKPTFLKPTIN 331

[Close]
BLAST of Lsi02G022230 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 320.9 bits (821), Expect = 1.2e-87
Identity = 158/302 (52.32%), Postives = 196/302 (64.90%), Query Frame = 0 

Query: 17  MVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTG-FAI 76
           +V K    ++  ++W   D       L +HLL +LAPF F+W    +A GL  +TG   I
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 77  TVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 136
           T+S+HRNL+H++FKLPKWLEY  AY  A A+QG+PIDWVSTHR HH+F DS+RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 137 GFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFI 196
           GFWFS M W FD+  +T++                       G P+NV DLEKQ FYRF+
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 197 HKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNT---- 256
             TY LHP+AL+V L+A+GG PFI+WGM VR V    IT +VNS CH+WGKQ WNT    
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 257 -------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEK 301
                        GWHNNHHAFE+SARHG EWWQ+D  WY +KFLQ IG+AT+VKLPSE 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 363

[Close]
BLAST of Lsi02G022230 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 285.4 bits (729), Expect = 5.5e-77
Identity = 141/301 (46.84%), Postives = 181/301 (60.13%), Query Frame = 0 

Query: 16  EMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTGFAI 75
           E+V+    R F  + WT +D +    F+  HLL +LAPFYFSW  F +   L  + G  I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 76  TVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 135
           T+SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  +++RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 136 GFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFI 195
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRF+
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 196 HKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNT---- 255
            +T  LH +A S L +  GG+P ++WG+ +   + L +T +VNSVCHIWG + WNT    
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 256 -------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEK 300
                        GWHNNHHAFE+SARHG EWWQ+D  W  I+FL+ IG+ATNVKLP+E 
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

[Close]
BLAST of Lsi02G022230 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 282.3 bits (721), Expect = 4.7e-76
Identity = 141/311 (45.34%), Postives = 181/311 (58.20%), Query Frame = 0 

Query: 7   EEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALG 66
           E   K    +  M    R    +KW + D   AF  L +H LC+LAPF F+W    +AL 
Sbjct: 10  ENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALI 69

Query: 67  LANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 126
           +  + G  ITVSYHRNLAHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS
Sbjct: 70  VYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDS 129

Query: 127 ERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDL 186
           +RDPHSP  GFWFS ++W FD+  L  K                       G   NV DL
Sbjct: 130 DRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNVEDL 189

Query: 187 EKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGK 246
           ++Q +Y+F+ +T   H +    LL+  GG+ F+ WGM +   +   +T ++NS+CH+WG 
Sbjct: 190 KRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGS 249

Query: 247 QPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVA 301
           + W T                  WHNNHHAFE SAR G EWWQID  WY ++FL++IG+A
Sbjct: 250 RTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLA 298

[Close]
BLAST of Lsi02G022230 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 282.0 bits (720), Expect = 6.1e-76
Identity = 139/293 (47.44%), Postives = 176/293 (60.07%), Query Frame = 0 

Query: 25  LFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTCFLLALGLANLTGFAITVSYHRNLA 84
           +F  ++W + D         +H L +LAPFYF+W+   +      + G  ITVSYHRNLA
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 89

Query: 85  HRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMMW 144
           HRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DSERDPHSP  GFWFS ++W
Sbjct: 90  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 149

Query: 145 FFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYPDNVRDLEKQRFYRFIHKTYWLHPI 204
            +DS  L  K                       G   NV DL++Q FYRF+ KT   H +
Sbjct: 150 IYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHIL 209

Query: 205 ALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSVCHIWGKQPWNT------------- 264
            L   LF +GG+ F+ WGM V   L + +T ++NS+CHIWG + W T             
Sbjct: 210 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 269

Query: 265 ----GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFLQVIGVATNVKLPSEKQK 301
                WHNNHHAFE SAR G EWWQID  WY ++F ++IG+AT+VK+P+E Q+
Sbjct: 270 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQR 300

[Close]
BLAST of Lsi02G022230 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-73
Identity = 142/317 (44.79%), Postives = 186/317 (58.68%), Query Frame = 0 

Query: 1   MGKEIMEEASKEEPKEMVMKSPPRLFKRKKWTKFDRNVAFYFLLLHLLCILAPFYFSWTC 60
           MG +  +++S +      ++   R F  +KWT+ D         +HLLC+LAPF ++W  
Sbjct: 1   MGDKNKDDSSSQ---SKAVRKEKRAFLFRKWTRVDVMRVSAVGAVHLLCLLAPFNYTWEA 60

Query: 61  FLLALGLANLTGFAITVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCH 120
           F  A  +   T  +IT SYHRNL HRSFKLPKWLEY  AY A  A+QG PIDWVSTHR H
Sbjct: 61  FRFAAMVGISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFH 120

Query: 121 HRFVDSERDPHSPIYGFWFSQMMWFFDSYNLTRKVCPNYFNNFEKVERNMFMFYMKHGYP 180
           H+F DS+RDPHSPI GFWFS + W FD+ +  R+ C                     G  
Sbjct: 121 HQFTDSDRDPHSPIEGFWFSHVFWIFDT-SYIREKC---------------------GGR 180

Query: 181 DNVRDLEKQRFYRFIHKTYWLHPIALSVLLFAIGGVPFIIWGMCVRFVLALQITSMVNSV 240
           DNV DL++Q FYRF+  T  LH +   +L++  GG+P++ W + V   +    T ++NS 
Sbjct: 181 DNVMDLKQQWFYRFLQNTIGLHILTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSA 240

Query: 241 CHIWGKQPWNT-----------------GWHNNHHAFEYSARHGHEWWQIDYGWYFIKFL 300
           CHIWG + WNT                  WHNNHHAFE SARHG EW+Q+D  WY I F 
Sbjct: 241 CHIWGSRAWNTKDTSRNIWWLGPFTMGESWHNNHHAFEASARHGLEWYQVDLTWYLIWFF 292

The following BLAST results are available for this feature:
BLAST of Lsi02G022230 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q949X01.7e-8652.32Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
Q9LVZ37.8e-7646.84Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
O657976.6e-7545.34Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
Q9SID28.6e-7547.44Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=... [more]
Q9LND81.8e-7246.18Delta-9 desaturase-like 2 protein OS=Arabidopsis thaliana OX=3702 GN=At1g06100 P... [more]
BLAST of Lsi02G022230 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BS495.3e-16886.02palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A0A0KAB13.8e-16684.50FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G3763... [more]
A0A1S3BQG55.5e-15780.79palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GPG59.0e-15280.56palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1JUL61.2e-15179.94palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
BLAST of Lsi02G022230 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038899717.11.2e-16986.63palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
XP_008451258.11.1e-16786.02PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
XP_011659261.25.1e-16584.19palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_011659770.12.5e-16484.24palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_008451259.11.1e-15680.79PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
BLAST of Lsi02G022230 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G15850.11.2e-8752.32fatty acid desaturase 5 [more]
AT3G15870.15.5e-7746.84Fatty acid desaturase family protein [more]
AT1G06080.14.7e-7645.34delta 9 desaturase 1 [more]
AT2G31360.16.1e-7647.4416:0delta9 desaturase 2 [more]
AT1G06120.11.3e-7344.79Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 80..100
score: 37.09
coord: 117..146
score: 44.04
coord: 250..264
score: 47.37
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 23..303
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 57..293
e-value: 2.35935E-56
score: 178.518
NoneNo IPR availablePANTHERPTHR11351:SF87FATTY ACID DESATURASE, TYPE 1, CORE-RELATEDcoord: 23..303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G022230.1Lsi02G022230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water