Lsi02G022180 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G022180 vs. ExPASy Swiss-Prot
Match: O81629 (Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.9e-44
Identity = 159/588 (27.04%), Postives = 277/588 (47.11%), Query Frame = 0

Query: 29  SIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFTRFQANPSRNLDTLVEKRPQISSRQRKIK 88
           S+  PS +    V+ P+ +P               +NP R   T ++K P  SS   + K
Sbjct: 9   SVKTPSDTPPLRVAVPDTQPL--------------SNPPR---TPMKKTP--SSTPSRSK 68

Query: 89  EKSELEEAFESADTVDEMVKAFKDMES-AFEERDLGLASLKIGLKLDQEGEDPEKTLLYA 148
                    + + TV     A  D++  + +  DLG   LK+       GE P K L YA
Sbjct: 69  PSPNRSTGKKDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYA 128

Query: 149 NRALQALDK-----------DDNRNPSLPVAMTLQLMGSVNYSLKRFSDSLGYLNRANRI 208
            RA ++ ++             +  P L +AM+L ++ ++  SL RF +++  L RA ++
Sbjct: 129 IRATKSFERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQV 188

Query: 209 LGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKKCLEIKELTLEKDSREL 268
                       D      +  ++L +  + +G+ + ++A  ++ L+I+  TL      +
Sbjct: 189 PDPTR-----GPDHSLAAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRV 248

Query: 269 GKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQ 328
           G+  R LAEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++
Sbjct: 249 GETCRYLAEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYE 308

Query: 329 KALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKDVV----QHTDKD 388
            ALE   L+   +   G  S++   ++   N+ ++L R+DEA+ + +  +        + 
Sbjct: 309 NALEHLVLASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGET 368

Query: 389 SETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKR-EATSPVEAAEAYSEISMQYEM 448
             T A VF+ + ++     K  ++K   E A  I++K    T+  E A   +EIS  YE 
Sbjct: 369 HPTVASVFVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYES 428

Query: 449 MNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAISYLEGAAETLR 508
           ++E E A+ LL+KS+ LLE  P +Q +   + AR+G +    G+ ++A +  E A   LR
Sbjct: 429 VDEPEEALKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLR 488

Query: 509 ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLS 568
            + G K    G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+
Sbjct: 489 AA-GEKSAFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLA 548

Query: 569 KAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKA 600
             Y AMG    AIE  EQV+   +    TA  +  + ++ L +L ++A
Sbjct: 549 ATYDAMGRIEDAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEA 571

BLAST of Lsi02G022180 vs. ExPASy Swiss-Prot
Match: Q9LII8 (Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 7.1e-44
Identity = 142/497 (28.57%), Postives = 238/497 (47.89%), Query Frame = 0

Query: 118 EERDLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDK--DDNRNPSLPVAMTLQLMGS 177
           E  +LG+  LK   +L   GE+  K L  A RA++  +K  +  +   L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 178 VNYSLKRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEAL 237
           +   L R++D++  L R+  I   + E G   ED      A  ++L ++   MG+ E ++
Sbjct: 209 IYAGLGRYNDAVPVLERSIEI--PMIEDG---EDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 238 ANLKKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQ--LGH 297
                 LEI+   L +    +G+  R LAEA+V  + F++A   C  AL+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 298 NSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALG 357
            S+E A DR+L+G+I     +++ ALE   L+   + +     D+   +    +  ++L 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 358 RYDEAI----NTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDK 417
           R+DEAI      L    Q   +   + A+V++ +  +     K  D+K   E A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 418 REATSPV-EAAEAYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGW 477
               +P+ E A  + EIS  Y+ MNE + A+ LL+++L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 478 LLLLTGKVQEAISYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMA 537
           +  + G   E+    + A    R S   K    G   N +G A ++      AA +F  A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 538 KDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELR-E 597
           K I++   GP+H D++    NL+  Y AMG    AIE  E VV   +    TA  E+  E
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 598 AQRMLEQLKRKAHGTSK 605
            QR+   LK    G SK
Sbjct: 629 KQRLAALLKEAGRGRSK 640

BLAST of Lsi02G022180 vs. ExPASy Swiss-Prot
Match: F4HSX9 (Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 2.2e-37
Identity = 150/565 (26.55%), Postives = 255/565 (45.13%), Query Frame = 0

Query: 67  SRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEERDLGLAS 126
           S NLD  V  R       +K   KS +         V        + E + E  +L    
Sbjct: 91  SSNLDLEVMPRDMEKQTGKKNVTKSNVGVGGMRKKKVGGTKLQNGNEEPSSENVELARFL 150

Query: 127 LKIGLKLDQEGEDPEKTLLYANRALQALDKD-DNRNPSLPVAMTLQLMGSVNYSLKRFSD 186
           L     L   G+   K L   +RA +  +   +N  P L   M L +  +V+  LK +++
Sbjct: 151 LNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHVTAAVHCKLKEYNE 210

Query: 187 SLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKKCLEIK 246
           ++  L R+  I   + E+G   E+      A  ++L +    +G+ E +++   + L I+
Sbjct: 211 AIPVLQRSVEI--PVVEEG---EEHALAKFAGLMQLGDTYAMVGQLESSISCYTEGLNIQ 270

Query: 247 ELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAHDRRLL 306
           +  L ++   +G+  R LAEA V  L F +A   C  AL IH++     S+  A DRRL+
Sbjct: 271 KKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGLPGSIAEAADRRLM 330

Query: 307 GVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKD- 366
           G+I     +H+ ALE   L+   +   G  S++   +    +  ++L R+DEAI   +  
Sbjct: 331 GLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSLSRFDEAICAYQKS 390

Query: 367 -VVQHTDKDSETRAV--VFISMGKVLCNQEKFTDAKRCLEIASGIFDKRE-ATSPVEAAE 426
                T K     AV  V+I +  +     K  +AK   E A  I++      SP E A 
Sbjct: 391 LTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYESHNLEISPEEIAS 450

Query: 427 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 486
             ++IS+  E MNE E AI+LL+K+L +    P ++     + A++G L  + GK  E+ 
Sbjct: 451 GLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMGVLYYMMGKYMESY 510

Query: 487 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 546
           +  + A   LR +   +    G   N +G A ++L+  + A ++F  AK I++   GP+H
Sbjct: 511 NTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEEAKCILEQECGPYH 570

Query: 547 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 606
            +++    NL+ AY A+G    AI+    VV   +    TA     + +R L QL ++A 
Sbjct: 571 PETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTEDEKRRLAQLLKEAG 630

Query: 607 GTSKIELSTKALPLPHSSTSSKTLQ 626
             +  +  +    +    TSS  L+
Sbjct: 631 NVTGRKAKSLKTLIDSDLTSSSALR 650

BLAST of Lsi02G022180 vs. ExPASy Swiss-Prot
Match: Q6AZT7 (Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.7e-13
Identity = 81/379 (21.37%), Postives = 156/379 (41.16%), Query Frame = 0

Query: 202  EKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKKCLEIKELTLEKDSRELGKANR 261
            EK    E+    L  +   L      +G   +A+  L++ LEI+E  L+ D   + ++  
Sbjct: 903  EKNCEGEEKMTSLADLYETLGRFLKDLGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLH 962

Query: 262  DLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQ 321
             LA  Y+    F +A     +ALEI +   G   + VA +   L V+Y    + ++A + 
Sbjct: 963  QLAGVYMQSKKFGNAEQLYKQALEISENAYGSEHLRVARELDALAVLYQKQNKFEQAEQL 1022

Query: 322  NELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKDVVQHTDKDSETRAVVFI 381
             + S K+ +          A    +    AL R       L+       KD+   A    
Sbjct: 1023 RKKSLKIRQK--------SARRKGSMYGFALLRR----RALQLEELTLGKDTSDNARTLN 1082

Query: 382  SMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAEAYSEISMQYEMMNEFETAISL 441
             +G +   Q     A+  L+ +  + ++       + A++ + ++  Y    +++ A  L
Sbjct: 1083 ELGVLYYLQNNLETAETFLKRSLEMRERVLGADHPDCAQSINNLAALYNEKKQYDKAEEL 1142

Query: 442  LKKSLVLLEKLPQEQHSEGSVSAR-LGWLLLLTGKVQEAISYLEGAAETLRESFGSKHFG 501
             +++L +  +     H   + + + L  L    GK+ +A+   E A +  ++SFG KH  
Sbjct: 1143 YERALDIRRRALSPDHPSLAYTVKHLAVLYKRKGKLDKAVPLYELAVDIRQKSFGPKHPS 1202

Query: 502  VGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSY 561
            V     NL   Y ++++   A  ++  A  I + SLG  H    E  +NL+      G Y
Sbjct: 1203 VATALVNLAVLYCQMKKQDDALPLYERAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDY 1262

Query: 562  ALAIEFQEQVVDAWDSHGS 580
              A E  ++ ++  ++  S
Sbjct: 1263 EKAAELYKRAMEIKETETS 1269

BLAST of Lsi02G022180 vs. ExPASy Swiss-Prot
Match: A0JM23 (Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 1.7e-13
Identity = 77/353 (21.81%), Postives = 150/353 (42.49%), Query Frame = 0

Query: 228  MGRREEALANLKKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIH 287
            +G   +A+  L++ LEI+E  L+ D   + ++   LA  YV    F +A     +ALEI 
Sbjct: 940  LGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYVQSKKFGNAEQLYKQALEIS 999

Query: 288  KKQLGHNSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAAN 347
            +   G   + VA +   L V+Y    + ++A +  + S K+ +          A    + 
Sbjct: 1000 ENAYGSEHMRVARELDALAVLYQKQNKFEQAEQLRKKSLKIRQK--------SARRKGSM 1059

Query: 348  MQIALGRYDEAINTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIF 407
               AL R       L+       KD+   A     +G +   Q     A+  L+ +  + 
Sbjct: 1060 YGFALLRR----RALQLEELTLGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMR 1119

Query: 408  DKREATSPVEAAEAYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSAR-L 467
            ++       + A++ + ++  Y    +++ A  L +++L +  +     H   + + + L
Sbjct: 1120 ERVLGADHPDCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHL 1179

Query: 468  GWLLLLTGKVQEAISYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA 527
              L    GK+ +A+   E A E  ++SFG KH  V     NL   Y ++++   A+ ++ 
Sbjct: 1180 AVLYKRKGKLDKAVPLYELAVEIRQKSFGPKHPSVATALVNLAVLYCQMKKQAEASPLYE 1239

Query: 528  MAKDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGS 580
             A  I + SLG  H    E  +NL+      G +  A E  ++ ++  ++  S
Sbjct: 1240 RAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDFEKAAELYKRAMEIKETETS 1280

BLAST of Lsi02G022180 vs. ExPASy TrEMBL
Match: A0A6J1GPX7 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456463 PE=4 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 572/633 (90.36%), Postives = 600/633 (94.79%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASISL  +SNR+NLRSLSSL+SP  I +PS S SSPVS P+L PCTKNHGLFFK +
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKLS 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQRK KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL+ALDKDDN  PSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQLEE GFSAE +RPVLHAVQLELANVKTAMGRREEALANL+K
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALP+CM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDK+EA SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE MNEFETAISLLK+SL LLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELER QSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAW+ HGS AEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQK 634
           G SKIELS KALPLPHSSTSSKTLQPDIP+ QK
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQK 633

BLAST of Lsi02G022180 vs. ExPASy TrEMBL
Match: A0A0A0K8P3 (TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375840 PE=4 SV=1)

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 568/633 (89.73%), Postives = 599/633 (94.63%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASISL    NRLNLRSLSSL+SP+SI N   SNSSPVSTPNLKPCT N+GLFFKFT
Sbjct: 12  MRRNASISLLPYFNRLNLRSLSSLISPNSISN-LPSNSSPVSTPNLKPCTINNGLFFKFT 71

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNLDTLVEKRP+ISSRQRK+K+KS LEE+FESA+TVDEM KAFK+MESAFEE+
Sbjct: 72  RFQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESAETVDEMFKAFKEMESAFEEK 131

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKI LKLD+EGEDPE+ LLYA+RAL+ALDKDDNRNPSLPVAMTLQL+GSVNYSL
Sbjct: 132 DLGLASLKIALKLDREGEDPEQVLLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 191

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQLEEKG+SAED+RPVLHAV LELANVKTAMGRREEAL+NL+K
Sbjct: 192 KRFSDSLGYLNRANRILGQLEEKGYSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRK 251

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
           CLEIKEL LEKDSRELG ANRDLAEAYVAILNFKDALP+CMK LEIHKK+LG+NSVEVAH
Sbjct: 252 CLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 311

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEE+QKALEQNELS+KVLKNWGLNSDLLRAE DAANMQIALGRYDEAIN
Sbjct: 312 DRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAIN 371

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDV QHTDKDSE R VVF SMGKVLCNQEKFTDAKRCLEIASGIFDKRE TSPVEAAE
Sbjct: 372 TLKDVAQHTDKDSELRGVVFFSMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAE 431

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE MNEF+TAISLLKKSL LLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI
Sbjct: 432 AYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 491

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGS HFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHH
Sbjct: 492 PYLEGAAETLKESFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 551

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEAC NLSKAYGAMGSYALAIEFQEQVVDAWDSHGS+AEDELREA+R LEQLKRKA 
Sbjct: 552 ADSIEACHNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAR 611

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQK 634
           GTSK E STKALPLP SSTSSKTLQPD+P RQ+
Sbjct: 612 GTSKFEHSTKALPLPQSSTSSKTLQPDLPTRQQ 643

BLAST of Lsi02G022180 vs. ExPASy TrEMBL
Match: A0A1S3BR15 (nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 568/633 (89.73%), Postives = 600/633 (94.79%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASIS+    NRLNLRSLSSL+SP+SI N    NSSPVSTPNLKPCTKN+GLFFKFT
Sbjct: 1   MRRNASISILPYFNRLNLRSLSSLISPNSISN-LPLNSSPVSTPNLKPCTKNNGLFFKFT 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQAN SRNLDTLVEKRP++SSRQRK+KEKS+LEEAFESADTVDEMVKAFK+MESAFEE+
Sbjct: 61  RFQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEK 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKLDQEGEDPE+ LLYA+RAL+ALDKDDNRNPSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEAL+NL+K
Sbjct: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
            LEIKEL LEKDSRELG ANRDLAEAYVAILNFKDALP+CMK LEIHKK+LG+NSVEVAH
Sbjct: 241 SLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELS+KVLKNWGLNSDLLRAEI+AANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDVVQHTDKDSE R VVF SMGKVLCNQEKFTD KRCLEIA GIFDKRE TSP  AAE
Sbjct: 361 TLKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAE 420

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISM YE MN+FETAISLLK+SL LLEKLPQEQHSEGSVSARLGWLLLL GKVQEAI
Sbjct: 421 AYSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAI 480

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEAC NLSKAYGAMGSYALAIEFQ+QVVDAW+SHGSTAEDELREA++ L+QLKRKA 
Sbjct: 541 ADSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQ 600

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQK 634
           GTSK ELSTKALPLPHSSTSSKTLQPD+PA QK
Sbjct: 601 GTSKFELSTKALPLPHSSTSSKTLQPDLPAHQK 632

BLAST of Lsi02G022180 vs. ExPASy TrEMBL
Match: A0A5A7UWT8 (Nephrocystin-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00740 PE=4 SV=1)

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 568/633 (89.73%), Postives = 600/633 (94.79%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASIS+    NRLNLRSLSSL+SP+SI N    NSSPVSTPNLKPCTKN+GLFFKFT
Sbjct: 1   MRRNASISVLPYFNRLNLRSLSSLISPNSISN-LPLNSSPVSTPNLKPCTKNNGLFFKFT 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQAN SRNLDTLVEKRP++SSRQRK+KEKS+LEEAFESADTVDEMVKAFK+MESAFEE+
Sbjct: 61  RFQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEK 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKLDQEGEDPE+ LLYA+RAL+ALDKDDNRNPSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEAL+NL+K
Sbjct: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
            LEIKEL LEKDSRELG ANRDLAEAYVAILNFKDALP+CMK LEIHKK+LG+NSVEVAH
Sbjct: 241 SLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELS+KVLKNWGLNSDLLRAEI+AANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDVVQHTDKDSE R VVF SMGKVLCNQEKFTD KRCLEIA GIFDKRE TSP  AAE
Sbjct: 361 TLKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAE 420

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISM YE MN+FETAISLLK+SL LLEKLPQEQHSEGSVSARLGWLLLL GKVQEAI
Sbjct: 421 AYSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAI 480

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEAC NLSKAYGAMGSYALAIEFQ+QVVDAW+SHGSTAEDELREA++ L+QLKRKA 
Sbjct: 541 ADSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQ 600

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQK 634
           GTSK ELSTKALPLPHSSTSSKTLQPD+PA QK
Sbjct: 601 GTSKFELSTKALPLPHSSTSSKTLQPDLPAHQK 632

BLAST of Lsi02G022180 vs. ExPASy TrEMBL
Match: A0A6J1JWL0 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC111488134 PE=4 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 569/633 (89.89%), Postives = 596/633 (94.15%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASISL  +SNR NLRSLSSL+SP  I +PS S SSPVST +L PCTKNHGLFFKF 
Sbjct: 1   MRRNASISLLFHSNRFNLRSLSSLISPKFISDPSFSYSSPVSTTHLNPCTKNHGLFFKFK 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQRK KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL+ALDKDDN   SLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKLSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQLEE GFSAE +RPVLHAVQLELANVKTAMGRREEALANL+K
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKA RDLAEAYVAILNFKDAL +CM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKAKRDLAEAYVAILNFKDALLFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDK+EA SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE MNEFETAISLLK+SL LLEKLPQEQHS GSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSVGSVSARLGWLLLLTGKVHEAI 480

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAW+ HGS AEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQK 634
           G SKIELS KALPLPHSSTSSKTLQPDIP+ Q+
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQE 633

BLAST of Lsi02G022180 vs. NCBI nr
Match: XP_038899312.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida])

HSP 1 Score: 1140.9 bits (2950), Expect = 0.0e+00
Identity = 597/634 (94.16%), Postives = 615/634 (97.00%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASISL S+SNRLNLRSLSS +S +SI NPS SNSSPVSTPNLKPCTKNHGLFFKFT
Sbjct: 1   MRRNASISLLSHSNRLNLRSLSSFISSNSISNPSPSNSSPVSTPNLKPCTKNHGLFFKFT 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNLDTLVEKRPQISSRQRK+KEKSELEEAFESADTVDEMVKAFKDMESAFEER
Sbjct: 61  RFQANPSRNLDTLVEKRPQISSRQRKVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKLDQEGEDPE  LLYANRAL+ALDKD N NPSLPVAMTLQLMGSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPENILLYANRALKALDKDVNGNPSLPVAMTLQLMGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQL EKGFSAEDVRPVLHAVQLELANVKTAMGRREEAL+NL+K
Sbjct: 181 KRFSDSLGYLNRANRILGQLVEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELG ANRDLAEAYVA+LNFKDALP+CMKALEIHK QLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGNANRDLAEAYVAVLNFKDALPFCMKALEIHKMQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLL VIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRY+EAI+
Sbjct: 301 DRRLLAVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYNEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTD KRCLEIASGIFDKREATSPVEAAE
Sbjct: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDPKRCLEIASGIFDKREATSPVEAAE 420

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE MNEFETAISLLKKSL LLEKLP EQHSEGSVSARLGWLLLLTGKVQEAI
Sbjct: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPHEQHSEGSVSARLGWLLLLTGKVQEAI 480

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEAC+NLSKAYGAMGSY LAIEFQEQVVDAW+SHGSTAEDELREAQ+ML+QLKRKAH
Sbjct: 541 ADSIEACRNLSKAYGAMGSYPLAIEFQEQVVDAWESHGSTAEDELREAQQMLKQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQKQ 635
           GTSKIELSTKALPLPHSSTSSKTLQPD+PARQK+
Sbjct: 601 GTSKIELSTKALPLPHSSTSSKTLQPDLPARQKR 634

BLAST of Lsi02G022180 vs. NCBI nr
Match: XP_023549125.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 573/633 (90.52%), Postives = 601/633 (94.94%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASISL  +SNR+NLRSLSSL+SP  I +PS S SSPVS P+L PCTKNHGLFFKF 
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKFN 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQA+PSRNL+TLVEKRPQISSRQRK KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQASPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL+ALDKDDN  PSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQLEE GFSAE +RPVLHAVQLELANVKTAMGRREEALANL+K
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALP+CM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDK+EA SPV AAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVAAAE 420

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE MNEFETAISLLK+SL LLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAW+ HGSTAEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSTAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQK 634
           G SKIELS KALPLPHSSTSSKTLQPDIP+ QK
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQK 633

BLAST of Lsi02G022180 vs. NCBI nr
Match: XP_022954096.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita moschata])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 572/633 (90.36%), Postives = 600/633 (94.79%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASISL  +SNR+NLRSLSSL+SP  I +PS S SSPVS P+L PCTKNHGLFFK +
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKLS 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQRK KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL+ALDKDDN  PSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQLEE GFSAE +RPVLHAVQLELANVKTAMGRREEALANL+K
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALP+CM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDK+EA SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE MNEFETAISLLK+SL LLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELER QSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++GSYALAIEFQEQVVDAW+ HGS AEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQK 634
           G SKIELS KALPLPHSSTSSKTLQPDIP+ QK
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQK 633

BLAST of Lsi02G022180 vs. NCBI nr
Match: KAG7014584.1 (Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 569/633 (89.89%), Postives = 600/633 (94.79%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASISL  +SNR+NLRSLSSL+SP  I +PS S SSPVS  +L PCTKNHGLFFKF+
Sbjct: 11  MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSASHLNPCTKNHGLFFKFS 70

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQRK KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 71  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 130

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL+ALDKDDN  PSLPVAMTLQL+GSVNYSL
Sbjct: 131 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 190

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQLEE GFSAE +RPVLHAVQLELANVKTAMGRREEALA+L+K
Sbjct: 191 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALASLRK 250

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSREL KANRDLAEAYVAILNFKDALP+CM+ALEIHKKQLGHNSVEVAH
Sbjct: 251 CLEIKELTLEKDSRELAKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 310

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGR+DEAI+
Sbjct: 311 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRFDEAIS 370

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDK+EA+SPVEAAE
Sbjct: 371 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEASSPVEAAE 430

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE MNEFETAISLLK+SL LLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 431 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 490

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 491 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 550

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++ SYALAIEFQEQVVDAW+ HGS AEDELREAQRMLEQLKRKAH
Sbjct: 551 ADSIEACQNLAKAYGSLESYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 610

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQK 634
           G SKIELS KALPLPHSSTSSKTLQPDIP+ QK
Sbjct: 611 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQK 643

BLAST of Lsi02G022180 vs. NCBI nr
Match: KAG6576067.1 (Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 569/633 (89.89%), Postives = 600/633 (94.79%), Query Frame = 0

Query: 1   MRRNASISLFSNSNRLNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFT 60
           MRRNASISL  +SNR+NLRSLSSL+SP  I +PS S SSPVS  +L PCTKNHGLFFKF+
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSASHLNPCTKNHGLFFKFS 60

Query: 61  RFQANPSRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120
           RFQANPSRNL+TLVEKRPQISSRQRK KEKSELEEAFESA+TVDEMVKAFKDMES FEER
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSL 180
           DLGLASLKIGLKL+QE EDPEK L+YA RAL+ALDKDDN  PSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKK 240
           KRFSDSLGYLNRANRILGQLEE GFSAE +RPVLHAVQLELANVKTAMGRREEALA+L+K
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALASLRK 240

Query: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAH 300
           CLEIKELTLEKDSREL KANRDLAEAYVAILNFKDALP+CM+ALEIHKKQLGHNSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELAKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGR+DEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRFDEAIS 360

Query: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAE 420
           TLKDVVQHT+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDK+EA+SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEASSPVEAAE 420

Query: 421 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE MNEFETAISLLK+SL LLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540
            YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 600
           ADSIEACQNL+KAYG++ SYALAIEFQEQVVDAW+ HGS AEDELREAQRMLEQLKRKAH
Sbjct: 541 ADSIEACQNLAKAYGSLESYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKIELSTKALPLPHSSTSSKTLQPDIPARQK 634
           G SKIELS KALPLPHSSTSSKTLQPDIP+ QK
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQK 633

BLAST of Lsi02G022180 vs. TAIR 10
Match: AT2G31240.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 758.8 bits (1958), Expect = 3.4e-219
Identity = 403/608 (66.28%), Postives = 494/608 (81.25%), Query Frame = 0

Query: 16  LNLRSLSSLVSPSSIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFTRFQANPSRNLDTLVE 75
           ++L + SS  S SS+ +P+   S+         C + + L  +  +F  NP RNL+T VE
Sbjct: 24  IHLNAASSSSSSSSLISPAREIST--------SCRRTNDLIKR--QFWVNPYRNLNTHVE 83

Query: 76  KRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEERDLGLASLKIGLKLDQ 135
           + P+ISS     KEK +LEEAFESA+T DEMV+ FK+ME +FE  +LGL++LK+GL LD+
Sbjct: 84  EPPEISSSD---KEKIDLEEAFESANTTDEMVRLFKEMELSFEGNELGLSALKLGLHLDR 143

Query: 136 EGEDPEKTLLYANRALQALDKDDNRNPSLPVAMTLQLMGSVNYSLKRFSDSLGYLNRANR 195
           EGEDPEK L YA++AL++ D D N+ P+L VAM  QLMGS NY LKRFSDSLGYLNRANR
Sbjct: 144 EGEDPEKVLSYADKALKSFDGDGNK-PNLLVAMASQLMGSANYGLKRFSDSLGYLNRANR 203

Query: 196 ILGQLEEKG-FSAEDVRPVLHAVQLELANVKTAMGRREEALANLKKCLEIKELTLEKDSR 255
           IL +LE  G    EDVRPVLHAVQLELANVK AMGRREEA+ NLKK LEIKE+T ++DS+
Sbjct: 204 ILVKLEADGDCVVEDVRPVLHAVQLELANVKNAMGRREEAIENLKKSLEIKEMTFDEDSK 263

Query: 256 ELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEE 315
           E+G ANR LA+AYVA+LNF +ALPY +KALEIHKK+LG+NS EVA DRRLLGVIYSGLE+
Sbjct: 264 EMGVANRSLADAYVAVLNFNEALPYALKALEIHKKELGNNSAEVAQDRRLLGVIYSGLEQ 323

Query: 316 HQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKDVVQHTDKDSE 375
           H KALEQN LSQ+VLKNWG+  +L+RAEIDAANM++ALG+Y+EAI+ LK VVQ TDKDSE
Sbjct: 324 HDKALEQNRLSQRVLKNWGMKLELIRAEIDAANMKVALGKYEEAIDILKSVVQQTDKDSE 383

Query: 376 TRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREATSPVEAAEAYSEISMQYEMMNE 435
            RA+VFISM K L NQ+KF ++KRCLE A  I +K+E   PVE AEAYSE++MQYE MNE
Sbjct: 384 MRAMVFISMSKALVNQQKFAESKRCLEFACEILEKKETALPVEVAEAYSEVAMQYESMNE 443

Query: 436 FETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAISYLEGAAETLRESF 495
           FETAISLL+K+L +LEKLPQEQHSEGSVSAR+GWLLL +G+V +A+ YLE AAE L+ESF
Sbjct: 444 FETAISLLQKTLGILEKLPQEQHSEGSVSARIGWLLLFSGRVSQAVPYLESAAERLKESF 503

Query: 496 GSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLSKAY 555
           G+KHFGVGY+YNNLGAAYLEL RPQSAAQMFA+AKDIMDVSLGP+H DSI+ACQNLSKAY
Sbjct: 504 GAKHFGVGYVYNNLGAAYLELGRPQSAAQMFAVAKDIMDVSLGPNHVDSIDACQNLSKAY 563

Query: 556 GAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH-GTSKIELSTKALP 615
             MG+Y+LA+EFQ++V++AWD+HG +A+DE++EA+R+LE L+ KA  G S  +L  KALP
Sbjct: 564 AGMGNYSLAVEFQQRVINAWDNHGDSAKDEMKEAKRLLEDLRLKARGGVSTNKLLNKALP 617

Query: 616 LPHSSTSS 622
           LP  S SS
Sbjct: 624 LPKPSHSS 617

BLAST of Lsi02G022180 vs. TAIR 10
Match: AT4G10840.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 1.3e-45
Identity = 159/588 (27.04%), Postives = 277/588 (47.11%), Query Frame = 0

Query: 29  SIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFTRFQANPSRNLDTLVEKRPQISSRQRKIK 88
           S+  PS +    V+ P+ +P               +NP R   T ++K P  SS   + K
Sbjct: 9   SVKTPSDTPPLRVAVPDTQPL--------------SNPPR---TPMKKTP--SSTPSRSK 68

Query: 89  EKSELEEAFESADTVDEMVKAFKDMES-AFEERDLGLASLKIGLKLDQEGEDPEKTLLYA 148
                    + + TV     A  D++  + +  DLG   LK+       GE P K L YA
Sbjct: 69  PSPNRSTGKKDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYA 128

Query: 149 NRALQALDK-----------DDNRNPSLPVAMTLQLMGSVNYSLKRFSDSLGYLNRANRI 208
            RA ++ ++             +  P L +AM+L ++ ++  SL RF +++  L RA ++
Sbjct: 129 IRATKSFERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQV 188

Query: 209 LGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKKCLEIKELTLEKDSREL 268
                       D      +  ++L +  + +G+ + ++A  ++ L+I+  TL      +
Sbjct: 189 PDPTR-----GPDHSLAAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRV 248

Query: 269 GKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQ 328
           G+  R LAEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++
Sbjct: 249 GETCRYLAEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYE 308

Query: 329 KALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKDVV----QHTDKD 388
            ALE   L+   +   G  S++   ++   N+ ++L R+DEA+ + +  +        + 
Sbjct: 309 NALEHLVLASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGET 368

Query: 389 SETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKR-EATSPVEAAEAYSEISMQYEM 448
             T A VF+ + ++     K  ++K   E A  I++K    T+  E A   +EIS  YE 
Sbjct: 369 HPTVASVFVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYES 428

Query: 449 MNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAISYLEGAAETLR 508
           ++E E A+ LL+KS+ LLE  P +Q +   + AR+G +    G+ ++A +  E A   LR
Sbjct: 429 VDEPEEALKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLR 488

Query: 509 ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLS 568
            + G K    G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+
Sbjct: 489 AA-GEKSAFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLA 548

Query: 569 KAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKA 600
             Y AMG    AIE  EQV+   +    TA  +  + ++ L +L ++A
Sbjct: 549 ATYDAMGRIEDAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEA 571

BLAST of Lsi02G022180 vs. TAIR 10
Match: AT3G27960.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 5.0e-45
Identity = 142/497 (28.57%), Postives = 238/497 (47.89%), Query Frame = 0

Query: 118 EERDLGLASLKIGLKLDQEGEDPEKTLLYANRALQALDK--DDNRNPSLPVAMTLQLMGS 177
           E  +LG+  LK   +L   GE+  K L  A RA++  +K  +  +   L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 178 VNYSLKRFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEAL 237
           +   L R++D++  L R+  I   + E G   ED      A  ++L ++   MG+ E ++
Sbjct: 209 IYAGLGRYNDAVPVLERSIEI--PMIEDG---EDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 238 ANLKKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQ--LGH 297
                 LEI+   L +    +G+  R LAEA+V  + F++A   C  AL+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 298 NSVEVAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALG 357
            S+E A DR+L+G+I     +++ ALE   L+   + +     D+   +    +  ++L 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 358 RYDEAI----NTLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDK 417
           R+DEAI      L    Q   +   + A+V++ +  +     K  D+K   E A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 418 REATSPV-EAAEAYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGW 477
               +P+ E A  + EIS  Y+ MNE + A+ LL+++L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 478 LLLLTGKVQEAISYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMA 537
           +  + G   E+    + A    R S   K    G   N +G A ++      AA +F  A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 538 KDIMDVSLGPHHADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELR-E 597
           K I++   GP+H D++    NL+  Y AMG    AIE  E VV   +    TA  E+  E
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 598 AQRMLEQLKRKAHGTSK 605
            QR+   LK    G SK
Sbjct: 629 KQRLAALLKEAGRGRSK 640

BLAST of Lsi02G022180 vs. TAIR 10
Match: AT4G10840.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 169.9 bits (429), Expect = 6.8e-42
Identity = 148/547 (27.06%), Postives = 257/547 (46.98%), Query Frame = 0

Query: 29  SIYNPSSSNSSPVSTPNLKPCTKNHGLFFKFTRFQANPSRNLDTLVEKRPQISSRQRKIK 88
           S+  PS +    V+ P+ +P               +NP R   T ++K P  SS   + K
Sbjct: 9   SVKTPSDTPPLRVAVPDTQPL--------------SNPPR---TPMKKTP--SSTPSRSK 68

Query: 89  EKSELEEAFESADTVDEMVKAFKDMES-AFEERDLGLASLKIGLKLDQEGEDPEKTLLYA 148
                    + + TV     A  D++  + +  DLG   LK+       GE P K L YA
Sbjct: 69  PSPNRSTGKKDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYA 128

Query: 149 NRALQALDK-----------DDNRNPSLPVAMTLQLMGSVNYSLKRFSDSLGYLNRANRI 208
            RA ++ ++             +  P L +AM+L ++ ++  SL RF +++  L RA ++
Sbjct: 129 IRATKSFERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQV 188

Query: 209 LGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKKCLEIKELTLEKDSREL 268
                       D      +  ++L +  + +G+ + ++A  ++ L+I+  TL      +
Sbjct: 189 PDPTR-----GPDHSLAAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRV 248

Query: 269 GKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAHDRRLLGVIYSGLEEHQ 328
           G+  R LAEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +++
Sbjct: 249 GETCRYLAEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYE 308

Query: 329 KALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKDVV----QHTDKD 388
            ALE   L+   +   G  S++   ++   N+ ++L R+DEA+ + +  +        + 
Sbjct: 309 NALEHLVLASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGET 368

Query: 389 SETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKR-EATSPVEAAEAYSEISMQYEM 448
             T A VF+ + ++     K  ++K   E A  I++K    T+  E A   +EIS  YE 
Sbjct: 369 HPTVASVFVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYES 428

Query: 449 MNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAISYLEGAAETLR 508
           ++E E A+ LL+KS+ LLE  P +Q +   + AR+G +    G+ ++A +  E A   LR
Sbjct: 429 VDEPEEALKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLR 488

Query: 509 ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIEACQNLS 559
            + G K    G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+
Sbjct: 489 AA-GEKSAFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLA 530

BLAST of Lsi02G022180 vs. TAIR 10
Match: AT1G27500.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 158.7 bits (400), Expect = 1.6e-38
Identity = 150/565 (26.55%), Postives = 255/565 (45.13%), Query Frame = 0

Query: 67  SRNLDTLVEKRPQISSRQRKIKEKSELEEAFESADTVDEMVKAFKDMESAFEERDLGLAS 126
           S NLD  V  R       +K   KS +         V        + E + E  +L    
Sbjct: 91  SSNLDLEVMPRDMEKQTGKKNVTKSNVGVGGMRKKKVGGTKLQNGNEEPSSENVELARFL 150

Query: 127 LKIGLKLDQEGEDPEKTLLYANRALQALDKD-DNRNPSLPVAMTLQLMGSVNYSLKRFSD 186
           L     L   G+   K L   +RA +  +   +N  P L   M L +  +V+  LK +++
Sbjct: 151 LNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHVTAAVHCKLKEYNE 210

Query: 187 SLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALANLKKCLEIK 246
           ++  L R+  I   + E+G   E+      A  ++L +    +G+ E +++   + L I+
Sbjct: 211 AIPVLQRSVEI--PVVEEG---EEHALAKFAGLMQLGDTYAMVGQLESSISCYTEGLNIQ 270

Query: 247 ELTLEKDSRELGKANRDLAEAYVAILNFKDALPYCMKALEIHKKQLGHNSVEVAHDRRLL 306
           +  L ++   +G+  R LAEA V  L F +A   C  AL IH++     S+  A DRRL+
Sbjct: 271 KKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGLPGSIAEAADRRLM 330

Query: 307 GVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAINTLKD- 366
           G+I     +H+ ALE   L+   +   G  S++   +    +  ++L R+DEAI   +  
Sbjct: 331 GLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSLSRFDEAICAYQKS 390

Query: 367 -VVQHTDKDSETRAV--VFISMGKVLCNQEKFTDAKRCLEIASGIFDKRE-ATSPVEAAE 426
                T K     AV  V+I +  +     K  +AK   E A  I++      SP E A 
Sbjct: 391 LTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYESHNLEISPEEIAS 450

Query: 427 AYSEISMQYEMMNEFETAISLLKKSLVLLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 486
             ++IS+  E MNE E AI+LL+K+L +    P ++     + A++G L  + GK  E+ 
Sbjct: 451 GLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMGVLYYMMGKYMESY 510

Query: 487 SYLEGAAETLRESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 546
           +  + A   LR +   +    G   N +G A ++L+  + A ++F  AK I++   GP+H
Sbjct: 511 NTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEEAKCILEQECGPYH 570

Query: 547 ADSIEACQNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSTAEDELREAQRMLEQLKRKAH 606
            +++    NL+ AY A+G    AI+    VV   +    TA     + +R L QL ++A 
Sbjct: 571 PETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTEDEKRRLAQLLKEAG 630

Query: 607 GTSKIELSTKALPLPHSSTSSKTLQ 626
             +  +  +    +    TSS  L+
Sbjct: 631 NVTGRKAKSLKTLIDSDLTSSSALR 650