Lsi02G022040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G022040
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptiongibberellin 20-oxidase-like protein
Locationchr02: 28479240 .. 28481241 (-)
RNA-Seq ExpressionLsi02G022040
SyntenyLsi02G022040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATATACATTATAAGCAACAAAACAATTGATAAATGAAATTCCAAAAGCAATAATCACACTATTTGTTTACAAAGAAAATGTCATCAAAAGAATGGATAGAGGAAGTTCCAGTGATGGACATTGATTCTCAAAGCTTCAGCCAATCAGCTATATCTTCCCTACGCAGAGCATGCAAGGAATGGGGATTCTTCTTCATTAGAAACCATGGCGTTTCGAAGGACTTTTACCGAAAACTCATCTCCGACGCCGACCGACGACTGTTTTCTTTTCCCGACGAGACGAAGCAGAAACTGAAAGTGGGAGTTTCCTCTTATACCCCTAGATTTGTGGTGTCACCATCCATGGAGAGCTTCAAGGTTTTAGGGCCCAACTTCTCTGCTTCTGCTGTTGCTCATGGTTTTACTGACTCCTTATTTGGCCCTCAAGCTACTCAATTCAGGTCAGCTTTTCTTTCCTTTTTCTTTTTTATGAGTTTCGAACAGTTCATCAATGAGACTGTTACAATATGACTGTCAGCGTCAAAGCAAACAATGACATATGAATTTGAAATATGTCTCCTAATAAGTAAGATTTTAGATAGATGTTTTTTCTTTAAGATTTCTTTCTCTTTTTTTTTAGAAATTATTAGAAGAGACTGATACAATGTAGTTGAAGATTTTATATTATTAGGAATATGCGAAAGAGACTAAAGAATTATTGTTTCATTACATAGATCTAATGTTTTAAAGGGTAGCATGGCTAATTGAATTATTTTTCCATTTTTTAGGGTTTTTGTTTTTTTAGTCAATATTGTTTGTTGTTGACTATAGTACAAGTCGTGAAACAAAAAAATTATAACGGTTTTTTACAGTGTCATTGATACTATTCTTTTTCTTTGATGGTTGATTCAAAGACTGAAAAAGAGTCATGAAATATGGTTGTGACCACTTTAATTCCTTAAATGCTATACAACATGCTAACTTTGTAAAATTAAAGGTAGGTTAAGAAATCATTAATCATGATTTTTTTGTCAAAGTGAGTATTAGCTCAACCGTAAGTAGCATGTACTAGGTTAGAGTCTTGGAGGTGTGAATTCCCTCTACCCCATTTGTTGTACTAACGGAAAAAAAAAATATTTTAGCTTAGCGATAAGTAGTAAAATGTGCTAGGGTTAGAGGTTGAATCTCCTTTACTTCATTTGTTGTACTAAAGAAAAAAAAATTATTTTCTTCATTATTTAATCGTCGTTCTGTAATCATCTTATTTATATACATCTAGACACTAATGTGTTGAAAGTTGAATTTATTTGCAGGAATCTTCTAGACGAATATGGAAGCAAAATGCAAGAAATATCAAAAAGAGTAATACAACTATTACTAAAAACATTGGGAGATGATCTTGAAACCAAATTCTATGATTCTGAGTTCAGTAATAATTGCAATGGCTATCTAAGGATAAACAACTACACTCCTCAAGACTTGAACGACAAAAAAGAAGTCGATGCGTTGAAGAAGCACACCGACATCAGCTACTTGACGATCTTATTCCAAGACGACATTGGGGGGCTACAAATGAGATCAAAAGGAGGCGAGTGGTTCGACGTAAAGCCGAGTGAGGACGGTGATCTTGTGGTTAACATTGGGGATTTTATGGAGGCTTGGAGCAATGGGAGGGTGAAATCAGCTGAACATAGAGTTGTTTTGAGGAAAAATAATGTGAAGAATAGATTTTCTGTGGCTTTCTTTTTGACAAATGAAGATGATGATAATAAGGAAATATATGCTCCAAGTGAAGTTGTTGGAGAAGGGAACTCAAGGCTTTATCTCCCAATTTCAACCCAAGAATATAGGCTGTTTAGGGAAAAAAATGATGAGAGAATTGAATCCACACTCAAAGATTTTGCAGGAATATAAAGTCGTTGATCATCGATCTTTGAGATTATAATAGCTATTTTATGATCTACCTTAAATATATATTATAATTTGTACTTTTTTGTGGTTAAAAATAAATATATGTTTTTTT

mRNA sequence

AATATACATTATAAGCAACAAAACAATTGATAAATGAAATTCCAAAAGCAATAATCACACTATTTGTTTACAAAGAAAATGTCATCAAAAGAATGGATAGAGGAAGTTCCAGTGATGGACATTGATTCTCAAAGCTTCAGCCAATCAGCTATATCTTCCCTACGCAGAGCATGCAAGGAATGGGGATTCTTCTTCATTAGAAACCATGGCGTTTCGAAGGACTTTTACCGAAAACTCATCTCCGACGCCGACCGACGACTGTTTTCTTTTCCCGACGAGACGAAGCAGAAACTGAAAGTGGGAGTTTCCTCTTATACCCCTAGATTTGTGGTGTCACCATCCATGGAGAGCTTCAAGGTTTTAGGGCCCAACTTCTCTGCTTCTGCTGTTGCTCATGGTTTTACTGACTCCTTATTTGGCCCTCAAGCTACTCAATTCAGGAATCTTCTAGACGAATATGGAAGCAAAATGCAAGAAATATCAAAAAGAGTAATACAACTATTACTAAAAACATTGGGAGATGATCTTGAAACCAAATTCTATGATTCTGAGTTCAGTAATAATTGCAATGGCTATCTAAGGATAAACAACTACACTCCTCAAGACTTGAACGACAAAAAAGAAGTCGATGCGTTGAAGAAGCACACCGACATCAGCTACTTGACGATCTTATTCCAAGACGACATTGGGGGGCTACAAATGAGATCAAAAGGAGGCGAGTGGTTCGACGTAAAGCCGAGTGAGGACGGTGATCTTGTGGTTAACATTGGGGATTTTATGGAGGCTTGGAGCAATGGGAGGGTGAAATCAGCTGAACATAGAGTTGTTTTGAGGAAAAATAATGTGAAGAATAGATTTTCTGTGGCTTTCTTTTTGACAAATGAAGATGATGATAATAAGGAAATATATGCTCCAAGTGAAGTTGTTGGAGAAGGGAACTCAAGGCTTTATCTCCCAATTTCAACCCAAGAATATAGGCTGTTTAGGGAAAAAAATGATGAGAGAATTGAATCCACACTCAAAGATTTTGCAGGAATATAAAGTCGTTGATCATCGATCTTTGAGATTATAATAGCTATTTTATGATCTACCTTAAATATATATTATAATTTGTACTTTTTTGTGGTTAAAAATAAATATATGTTTTTTT

Coding sequence (CDS)

ATGTCATCAAAAGAATGGATAGAGGAAGTTCCAGTGATGGACATTGATTCTCAAAGCTTCAGCCAATCAGCTATATCTTCCCTACGCAGAGCATGCAAGGAATGGGGATTCTTCTTCATTAGAAACCATGGCGTTTCGAAGGACTTTTACCGAAAACTCATCTCCGACGCCGACCGACGACTGTTTTCTTTTCCCGACGAGACGAAGCAGAAACTGAAAGTGGGAGTTTCCTCTTATACCCCTAGATTTGTGGTGTCACCATCCATGGAGAGCTTCAAGGTTTTAGGGCCCAACTTCTCTGCTTCTGCTGTTGCTCATGGTTTTACTGACTCCTTATTTGGCCCTCAAGCTACTCAATTCAGGAATCTTCTAGACGAATATGGAAGCAAAATGCAAGAAATATCAAAAAGAGTAATACAACTATTACTAAAAACATTGGGAGATGATCTTGAAACCAAATTCTATGATTCTGAGTTCAGTAATAATTGCAATGGCTATCTAAGGATAAACAACTACACTCCTCAAGACTTGAACGACAAAAAAGAAGTCGATGCGTTGAAGAAGCACACCGACATCAGCTACTTGACGATCTTATTCCAAGACGACATTGGGGGGCTACAAATGAGATCAAAAGGAGGCGAGTGGTTCGACGTAAAGCCGAGTGAGGACGGTGATCTTGTGGTTAACATTGGGGATTTTATGGAGGCTTGGAGCAATGGGAGGGTGAAATCAGCTGAACATAGAGTTGTTTTGAGGAAAAATAATGTGAAGAATAGATTTTCTGTGGCTTTCTTTTTGACAAATGAAGATGATGATAATAAGGAAATATATGCTCCAAGTGAAGTTGTTGGAGAAGGGAACTCAAGGCTTTATCTCCCAATTTCAACCCAAGAATATAGGCTGTTTAGGGAAAAAAATGATGAGAGAATTGAATCCACACTCAAAGATTTTGCAGGAATATAA

Protein sequence

MSSKEWIEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPDETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDEYGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDKKEVDALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRVKSAEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYRLFREKNDERIESTLKDFAGI
Homology
[Close]
BLAST of Lsi02G022040 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.4e-69
Identity = 153/333 (45.95%), Postives = 219/333 (65.77%), Query Frame = 0 

Query: 9   EVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFS---FP 68
           E+PV DI S+  S+S+++SL+ ACKEWGFF++ NHGVS+D Y+KL      R FS   F 
Sbjct: 4   ELPVFDI-SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKL------RRFSTGVFE 63

Query: 69  DETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQAT--QFRNL 128
            E ++K+K+G S+YTPRF+ SP  ES +V GP+F ASA +   +   F  QAT  +F  L
Sbjct: 64  LEDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGL 123

Query: 129 LDEYGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYT-PQDLNDKKE 188
           + EYG KM ++ +++++ +L + GDDL  K+Y+SEF  NC+GY RINNYT P D  D   
Sbjct: 124 MKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEF-GNCHGYFRINNYTIPSDQEDDHH 183

Query: 189 -------VDALKKHTDISYLTILFQDDIGGLQMRSKGG-EWFDVKPSEDGDLVVNIGDFM 248
                  ++ L  HTD+S +TI+ QDDIGGLQ+R++ G    D+ P ++  LVVN+GD +
Sbjct: 184 NGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEA-LVVNVGDLL 243

Query: 249 EAWSNGRVKSAEHRVVL-RKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVG--EGNSRLY 308
            AW+NGR++S++HRV+L R+  V NRFS+AFF     DD K ++AP EVVG  EG  R++
Sbjct: 244 HAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWC--FDDGKVVFAPDEVVGGCEG-MRVF 303

Query: 309 LPISTQEYRLFREKND----ERIESTLKDFAGI 321
                 +Y  FRE N+    E++  T++DFA I
Sbjct: 304 RSFKCGDYLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]
BLAST of Lsi02G022040 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.7e-28
Identity = 117/321 (36.45%), Postives = 162/321 (50.47%), Query Frame = 0 

Query: 8   EEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPDE 67
           E +PV+DI S    +S   ++  A +EWGFF + NHGVS +    +   A  R F  P E
Sbjct: 60  ESIPVIDI-SNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENM-KTATHRFFGLPVE 119

Query: 68  TKQKLKVGVS-SYTPRFVVSPSMESFKVL--GPNFSASAVAHGFTDSLFGPQATQFRNLL 127
            K+K     S S   RF  S S  + K L      S   V+      L+ P + +   L 
Sbjct: 120 EKRKFSREKSLSTNVRFGTSFSPHAEKALEWKDYLSLFFVSEAEASQLW-PDSCRSETL- 179

Query: 128 DEYGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRIN-NYTPQDLNDKKEV 187
            EY   M E +K +++ LL+ LG++L  K  D    +   G  RIN NY P   N +  V
Sbjct: 180 -EY---MNE-TKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPNPELTV 239

Query: 188 DALKKHTDISYLTILFQDDIGGLQMRS-KGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRV 247
             + +H+D+S LTIL QD+IGGL +RS   G W  V P   G LV+NIGD M+  SNGR 
Sbjct: 240 -GVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI-SGSLVINIGDAMQIMSNGRY 299

Query: 248 KSAEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAP-SEVVGEGNSRLYLPISTQEY-- 307
           KS EHRV+   N   NR SV  F++ + +    +  P  EV+  G   +Y  I   +Y  
Sbjct: 300 KSVEHRVL--ANGSYNRISVPIFVSPKPE---SVIGPLLEVIENGEKPVYKDILYTDYVK 359

Query: 308 RLFREKNDERIESTLKDFAGI 321
             FR+ +D +   T+ DFA I
Sbjct: 360 HFFRKAHDGK--KTI-DFANI 361

[Close]
BLAST of Lsi02G022040 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-27
Identity = 90/265 (33.96%), Postives = 136/265 (51.32%), Query Frame = 0 

Query: 9   EVPVMDID-----SQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFS 68
           E+PV+D+      ++   +    ++ RA +EWGFF + NHG+S D   K+  +   R+F 
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE-QIRVFR 100

Query: 69  FPDETKQKL-KVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAH-GFTDSLFGPQATQFR 128
            P + K K  K    SY      +PS  S + L    S S   H   TD       T   
Sbjct: 101 EPFDKKSKSEKFSAGSYR---WGTPSATSIRQL----SWSEAFHVPMTDISDNKDFTTLS 160

Query: 129 NLLDEYGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDKK 188
           + ++++ S+ + ++  + ++L +  G +  + F ++   N C  YLR+N Y P       
Sbjct: 161 STMEKFASESEALAYMLAEVLAEKSGQN-SSFFKENCVRNTC--YLRMNRYPP--CPKPS 220

Query: 189 EVDALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGR 248
           EV  L  HTD  +LTIL+QD +GGLQ+  K   W  VKP+    L++NIGD  +AWSNG 
Sbjct: 221 EVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKA-LIINIGDLFQAWSNGM 280

Query: 249 VKSAEHRVVLRKNNVKNRFSVAFFL 267
            KS EHRV+   N    RFS A+F+
Sbjct: 281 YKSVEHRVM--TNPKVERFSTAYFM 288

[Close]
BLAST of Lsi02G022040 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.6e-26
Identity = 96/314 (30.57%), Postives = 155/314 (49.36%), Query Frame = 0 

Query: 9   EVPVMDIDSQSFSQS-----AISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFS 68
           ++PV+D+   +  +       +  +  A KEWGFF I NHG+ KD +  ++ + +++LF 
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLE-EKKLFD 98

Query: 69  FPDETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTD--SLFGPQATQFR 128
            P   K + +    S       +PS  S       +S S   H      S         R
Sbjct: 99  QPFSVKVRERFSDLSKNSYRWGNPSATS----PAQYSVSEAFHIILSEVSRISDDRNNLR 158

Query: 129 NLLDEYGSKMQEISKRVIQLLLKTLGD--DLETKFYDSEFSNNCNGYLRINNYTPQDLND 188
            +++ Y   +QEI+ RV Q++ + LG   ++ ++++++ F    N +LR+N Y P     
Sbjct: 159 TIVETY---VQEIA-RVAQMICEILGKQVNVSSEYFENIFELE-NSFLRLNKYHPSVFG- 218

Query: 189 KKEVDALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSN 248
             EV  L  HTD S+LTIL QD IGGL++ +  G+W  VKP  +  L VNIGD  +A SN
Sbjct: 219 -SEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEA-LTVNIGDMFQALSN 278

Query: 249 GRVKSAEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEY 308
           G  +S  HRV+   N    R S+AFF+          Y  +E+   G  + Y   S +EY
Sbjct: 279 GVYQSVRHRVISPAN--IERMSIAFFVCP--------YLETEIDCFGYPKKYRRFSFREY 325

Query: 309 RLFREKNDERIEST 314
              +E+++  ++ T
Sbjct: 339 ---KEQSEHDVKET 325

[Close]
BLAST of Lsi02G022040 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.7e-26
Identity = 103/312 (33.01%), Postives = 151/312 (48.40%), Query Frame = 0 

Query: 8   EEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPDE 67
           E +PV+D+ +    + A  ++  A ++WGFF + NHGV  +     +  A  + F+ P E
Sbjct: 60  EAIPVIDMSNPDEDRVA-EAVCDAAEKWGFFQVINHGVPLEVLDD-VKAATHKFFNLPVE 119

Query: 68  TKQKL-KVGVSSYTPRF------VVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQF 127
            K+K  K    S T RF      +   ++E    L   F + A A  F   +        
Sbjct: 120 EKRKFTKENSLSTTVRFGTSFSPLAEQALEWKDYLSLFFVSEAEAEQFWPDI-------C 179

Query: 128 RNLLDEYGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRIN-NYTPQDLND 187
           RN   EY +K    SK++++ LL+ LG +L  K  D    +   G +R+N NY P   N 
Sbjct: 180 RNETLEYINK----SKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYPICPNP 239

Query: 188 KKEVDALKKHTDISYLTILFQDDIGGLQMRS-KGGEWFDVKPSEDGDLVVNIGDFMEAWS 247
              V  + +H+D+S LTIL QD IGGL +RS   G W  V P   G  V+NIGD M+  S
Sbjct: 240 DLTV-GVGRHSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVA-GSFVINIGDAMQIMS 299

Query: 248 NGRVKSAEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAP-SEVVGEGNSRLYLPISTQ 307
           NG  KS EHRV+   N   NR SV  F+  + +    +  P  EV+  G   +Y  +   
Sbjct: 300 NGLYKSVEHRVL--ANGYNNRISVPIFVNPKPE---SVIGPLPEVIANGEEPIYRDVLYS 351

[Close]
BLAST of Lsi02G022040 vs. ExPASy TrEMBL
Match: A0A6J1JW96 (gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488064 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 4.1e-120
Identity = 222/312 (71.15%), Postives = 264/312 (84.62%), Query Frame = 0 

Query: 7   IEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPD 66
           I EVPV+DI SQS ++SA SSL RAC E+GFFFIRNHG+SK+ Y+KLIS AD +LFS P+
Sbjct: 3   IVEVPVIDISSQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGAD-QLFSSPE 62

Query: 67  ETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDE 126
           ETKQKL +G SSY PRFV+SP  ESFKV GPNFSASA A GFT SLFG QAT+F NLLDE
Sbjct: 63  ETKQKLTIGASSYIPRFVLSPFFESFKVSGPNFSASANAQGFTQSLFGHQATEFSNLLDE 122

Query: 127 YGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLN-DKKEVDA 186
           YGSKM+E+SKRV++LLL  LGD++E KFYDSEFS NCN Y+RIN +TP+ +N +++E++ 
Sbjct: 123 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFS-NCNAYIRINGFTPRGMNEEEEELEV 182

Query: 187 LKKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRVKSA 246
            KKHTD+SY+TI+FQ+DI GLQMR KGG+W DV+PSED +LVVNIGDFMEAWSNGR++SA
Sbjct: 183 FKKHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPSEDNNLVVNIGDFMEAWSNGRLRSA 242

Query: 247 EHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYRLFREK 306
            HRVVLRKNN+K RFS+AFFLT +DDD KEIYAPSE+VGEG SRLY P STQ+YRLF+EK
Sbjct: 243 VHRVVLRKNNMK-RFSMAFFLTPKDDD-KEIYAPSEIVGEGKSRLYRPFSTQQYRLFKEK 302

Query: 307 NDERIESTLKDF 318
           ND      LKDF
Sbjct: 303 NDVNAGYQLKDF 310

[Close]
BLAST of Lsi02G022040 vs. ExPASy TrEMBL
Match: A0A6J1GMS4 (gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455930 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 4.6e-119
Identity = 222/319 (69.59%), Postives = 264/319 (82.76%), Query Frame = 0 

Query: 7   IEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPD 66
           IEE+PV+DI  QS ++SA SSL RAC E+GFFFIRNHG+SK+ Y+KLIS AD +LFS P+
Sbjct: 3   IEEIPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGAD-QLFSSPE 62

Query: 67  ETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDE 126
           ETKQKL +G SSYTPRFVVSP  ESFKV GPNFSASA A GFT SLFG QAT+F NLLDE
Sbjct: 63  ETKQKLTMGASSYTPRFVVSPFFESFKVSGPNFSASAAAQGFTQSLFGHQATEFSNLLDE 122

Query: 127 YGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLN----DKKE 186
           YGSKM+E+SKRV++LLL  LGD++E KFYDSEFS NCN YLRIN +TP+ +N    +++E
Sbjct: 123 YGSKMKEVSKRVVKLLLNILGDNIEDKFYDSEFS-NCNAYLRINGFTPRSMNEEEEEEEE 182

Query: 187 VDALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDV--KPSEDGDLVVNIGDFMEAWSNG 246
           ++  KKHTD+SY+TI+FQ+DI GLQMR KGG+W DV  +PSED +L+VNIGDFMEAWSNG
Sbjct: 183 LEVFKKHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPRPSEDDNLMVNIGDFMEAWSNG 242

Query: 247 RVKSAEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYR 306
           R++SA HRVVLRKNN+K RFS+AFFLT EDDD KEIYAPSE+VGEG SRLY P STQ+YR
Sbjct: 243 RLRSAVHRVVLRKNNMK-RFSMAFFLTTEDDD-KEIYAPSEIVGEGKSRLYRPFSTQQYR 302

Query: 307 LFREKNDERIESTLKDFAG 320
           LF+EK+       LKD  G
Sbjct: 303 LFKEKSGVNARYALKDIIG 317

[Close]
BLAST of Lsi02G022040 vs. ExPASy TrEMBL
Match: A0A6J1GPF2 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455932 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 5.0e-118
Identity = 214/314 (68.15%), Postives = 263/314 (83.76%), Query Frame = 0 

Query: 7   IEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPD 66
           IEEVPV+DI SQ+ ++S  SSL RAC E GFFFI+NHG+SK+FY+KLIS  D +LFS P+
Sbjct: 3   IEEVPVIDISSQTLTESTASSLSRACTELGFFFIKNHGISKEFYQKLISGID-QLFSSPE 62

Query: 67  ETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDE 126
           ETKQKLK GVSSYTPRFVVSP +ESFK+ GP FSASAVAHGFT S FG QAT+F NLLDE
Sbjct: 63  ETKQKLKQGVSSYTPRFVVSPFLESFKLSGPKFSASAVAHGFTQSQFGHQATEFSNLLDE 122

Query: 127 YGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDKKEVDAL 186
           YGSKM E+SK+V++LLLK LGD++E KFYD EFS NC  YLRIN+YTP  +ND++EV+  
Sbjct: 123 YGSKMTEVSKKVVKLLLKILGDNIENKFYDPEFS-NCTSYLRINSYTPLGMNDEEEVEVF 182

Query: 187 KKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRVKSAE 246
           KKHTD+SY TI+F +D+ GLQMRSKGG+W +V+PSEDG+LVVNIGD+MEAWSNGR++SA 
Sbjct: 183 KKHTDLSYATIVFPNDMRGLQMRSKGGDWINVRPSEDGNLVVNIGDYMEAWSNGRLRSAL 242

Query: 247 HRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYRLFREKN 306
           HRVVLRK N+  RFS+A FL+  +D+++EIYAPSEVVGEG SRLY   S Q+Y+ FRE N
Sbjct: 243 HRVVLRK-NITKRFSIA-FLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN 302

Query: 307 DERIESTLKDFAGI 321
            +++ +T+KDF GI
Sbjct: 303 YKKVGATIKDFVGI 312

[Close]
BLAST of Lsi02G022040 vs. ExPASy TrEMBL
Match: A0A6J1JSZ7 (gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488065 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.3e-117
Identity = 214/314 (68.15%), Postives = 261/314 (83.12%), Query Frame = 0 

Query: 7   IEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPD 66
           I+EVPV+D+  QS ++S  SSL RAC + GFFFIRNHGVSK+FY KLIS AD  LFS P+
Sbjct: 3   IKEVPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGAD-ELFSSPE 62

Query: 67  ETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDE 126
           ETKQ+LK+GVSSYTPRF+VSP  ESF+V GPNFSASA A GFT SLFG  AT+F NLLDE
Sbjct: 63  ETKQQLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLDE 122

Query: 127 YGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDKKEVDAL 186
           YG KM+E+SKRV++LLL  LGD++E KFYD EFS NCNGYLRIN +TP D+N ++E++  
Sbjct: 123 YGRKMKEVSKRVVKLLLNVLGDNIENKFYDPEFS-NCNGYLRINGFTPLDMNAEEEIEVF 182

Query: 187 KKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRVKSAE 246
           KKHTD+SY+TI+FQ+DI GLQMRSKGG+W DV+ SEDG LVVNIGD+MEAWSNGR++SA 
Sbjct: 183 KKHTDLSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDGILVVNIGDYMEAWSNGRLRSAV 242

Query: 247 HRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYRLFREKN 306
           HRVVLRKN +K RFS+A FL+  +D+++EIYAPSEVVGEG SRLY   S Q+Y+ FRE N
Sbjct: 243 HRVVLRKNIMK-RFSIA-FLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENN 302

Query: 307 DERIESTLKDFAGI 321
            E++ +T+KDF GI
Sbjct: 303 YEKVGATIKDFVGI 312

[Close]
BLAST of Lsi02G022040 vs. ExPASy TrEMBL
Match: A0A6J1GMT1 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455933 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 3.1e-115
Identity = 212/314 (67.52%), Postives = 254/314 (80.89%), Query Frame = 0 

Query: 7   IEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPD 66
           IEEVPV+DI SQS ++S  SSL RAC E GFFFI+NHG+SK+FY+K IS  D  LFS P+
Sbjct: 3   IEEVPVIDISSQSLTESTASSLFRACTELGFFFIKNHGISKEFYQKFISGID-LLFSSPE 62

Query: 67  ETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDE 126
           ETKQ+LK GVSSYTPRFV+SP +E+FK  GPNFSAS+ A G T S FG QAT+F NLLDE
Sbjct: 63  ETKQRLKHGVSSYTPRFVISPFLETFKFSGPNFSASSTAQGVTQSQFGHQATEFSNLLDE 122

Query: 127 YGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDKKEVDAL 186
           YGSKM E+SKR+++LLL  LGD++E KFYD EFS NCNGYLRIN YTP  +ND +EV+  
Sbjct: 123 YGSKMAEVSKRILKLLLNILGDNIENKFYDPEFS-NCNGYLRINGYTPLGMNDDEEVEVF 182

Query: 187 KKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRVKSAE 246
           KKHTD+SY+TI+F +DI GLQMRSKGG+W D  PSED  LVVNIGD +EAWSNGR++SA 
Sbjct: 183 KKHTDVSYVTIVFPNDIRGLQMRSKGGDWIDAMPSEDDTLVVNIGDLLEAWSNGRLRSAI 242

Query: 247 HRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYRLFREKN 306
           HRVVLRKNN+K RFS+ +  T E D+++EI+APSEVVGEG SRLY P STQ+Y+ FR KN
Sbjct: 243 HRVVLRKNNMK-RFSIVYTSTPE-DESREIHAPSEVVGEGKSRLYRPFSTQQYKDFRNKN 302

Query: 307 DERIESTLKDFAGI 321
            E+I  T+KDF GI
Sbjct: 303 YEKIGETIKDFVGI 312

[Close]
BLAST of Lsi02G022040 vs. NCBI nr
Match: XP_038899419.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 540.4 bits (1391), Expect = 1.0e-149
Identity = 266/321 (82.87%), Postives = 296/321 (92.21%), Query Frame = 0 

Query: 1   MSSKEWIEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRR 60
           MSSKEWIEE+PVMDI S SFS S ISSL +ACKEWGFFFIRNHG+ K+FY KLISDADRR
Sbjct: 1   MSSKEWIEEIPVMDIYSHSFSNSDISSLSKACKEWGFFFIRNHGIPKEFYGKLISDADRR 60

Query: 61  LFSFPDETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQF 120
           LFSFPDETK+K+KVGVSSYTPRFVVSPSMESFKVLGPNFSASA AHGFTDS+FG QAT+F
Sbjct: 61  LFSFPDETKRKMKVGVSSYTPRFVVSPSMESFKVLGPNFSASAAAHGFTDSIFGRQATEF 120

Query: 121 RNLLDEYGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDK 180
           RNLLDEYGSKM+E+SKRV+QLLLKTLG+DLE KFY+SEF  NC+GYLRIN+YT QDLND+
Sbjct: 121 RNLLDEYGSKMEEVSKRVVQLLLKTLGEDLENKFYESEF-GNCHGYLRINSYTLQDLNDE 180

Query: 181 KEVDALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNG 240
           +EV   KKHTD+SY+TILFQDD+GGLQMRSKGGEWFDVKPS+DG+L+VNIGDFMEAWSNG
Sbjct: 181 EEVVGFKKHTDLSYVTILFQDDVGGLQMRSKGGEWFDVKPSKDGNLLVNIGDFMEAWSNG 240

Query: 241 RVKSAEHRVVLRKN-NVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEY 300
           +V+SAEHRVVLRK  NVK RFSVAFFLT ED D +EIYAP+EVVGEG SRLYLP STQEY
Sbjct: 241 KVRSAEHRVVLRKKINVKKRFSVAFFLTYEDGD-REIYAPTEVVGEGKSRLYLPFSTQEY 300

Query: 301 RLFREKNDERIESTLKDFAGI 321
           RLFREKN E+I++TLKDFAGI
Sbjct: 301 RLFREKNYEKIDATLKDFAGI 319

[Close]
BLAST of Lsi02G022040 vs. NCBI nr
Match: XP_022991453.1 (gibberellin 20-oxidase-like protein isoform X1 [Cucurbita maxima])

HSP 1 Score: 441.0 bits (1133), Expect = 8.5e-120
Identity = 222/312 (71.15%), Postives = 264/312 (84.62%), Query Frame = 0 

Query: 7   IEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPD 66
           I EVPV+DI SQS ++SA SSL RAC E+GFFFIRNHG+SK+ Y+KLIS AD +LFS P+
Sbjct: 3   IVEVPVIDISSQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGAD-QLFSSPE 62

Query: 67  ETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDE 126
           ETKQKL +G SSY PRFV+SP  ESFKV GPNFSASA A GFT SLFG QAT+F NLLDE
Sbjct: 63  ETKQKLTIGASSYIPRFVLSPFFESFKVSGPNFSASANAQGFTQSLFGHQATEFSNLLDE 122

Query: 127 YGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLN-DKKEVDA 186
           YGSKM+E+SKRV++LLL  LGD++E KFYDSEFS NCN Y+RIN +TP+ +N +++E++ 
Sbjct: 123 YGSKMKEVSKRVVKLLLNILGDNIENKFYDSEFS-NCNAYIRINGFTPRGMNEEEEELEV 182

Query: 187 LKKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRVKSA 246
            KKHTD+SY+TI+FQ+DI GLQMR KGG+W DV+PSED +LVVNIGDFMEAWSNGR++SA
Sbjct: 183 FKKHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPSEDNNLVVNIGDFMEAWSNGRLRSA 242

Query: 247 EHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYRLFREK 306
            HRVVLRKNN+K RFS+AFFLT +DDD KEIYAPSE+VGEG SRLY P STQ+YRLF+EK
Sbjct: 243 VHRVVLRKNNMK-RFSMAFFLTPKDDD-KEIYAPSEIVGEGKSRLYRPFSTQQYRLFKEK 302

Query: 307 NDERIESTLKDF 318
           ND      LKDF
Sbjct: 303 NDVNAGYQLKDF 310

[Close]
BLAST of Lsi02G022040 vs. NCBI nr
Match: XP_022953356.1 (gibberellin 20-oxidase-like protein isoform X1 [Cucurbita moschata])

HSP 1 Score: 437.6 bits (1124), Expect = 9.4e-119
Identity = 222/319 (69.59%), Postives = 264/319 (82.76%), Query Frame = 0 

Query: 7   IEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPD 66
           IEE+PV+DI  QS ++SA SSL RAC E+GFFFIRNHG+SK+ Y+KLIS AD +LFS P+
Sbjct: 3   IEEIPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGAD-QLFSSPE 62

Query: 67  ETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDE 126
           ETKQKL +G SSYTPRFVVSP  ESFKV GPNFSASA A GFT SLFG QAT+F NLLDE
Sbjct: 63  ETKQKLTMGASSYTPRFVVSPFFESFKVSGPNFSASAAAQGFTQSLFGHQATEFSNLLDE 122

Query: 127 YGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLN----DKKE 186
           YGSKM+E+SKRV++LLL  LGD++E KFYDSEFS NCN YLRIN +TP+ +N    +++E
Sbjct: 123 YGSKMKEVSKRVVKLLLNILGDNIEDKFYDSEFS-NCNAYLRINGFTPRSMNEEEEEEEE 182

Query: 187 VDALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDV--KPSEDGDLVVNIGDFMEAWSNG 246
           ++  KKHTD+SY+TI+FQ+DI GLQMR KGG+W DV  +PSED +L+VNIGDFMEAWSNG
Sbjct: 183 LEVFKKHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPRPSEDDNLMVNIGDFMEAWSNG 242

Query: 247 RVKSAEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYR 306
           R++SA HRVVLRKNN+K RFS+AFFLT EDDD KEIYAPSE+VGEG SRLY P STQ+YR
Sbjct: 243 RLRSAVHRVVLRKNNMK-RFSMAFFLTTEDDD-KEIYAPSEIVGEGKSRLYRPFSTQQYR 302

Query: 307 LFREKNDERIESTLKDFAG 320
           LF+EK+       LKD  G
Sbjct: 303 LFKEKSGVNARYALKDIIG 317

[Close]
BLAST of Lsi02G022040 vs. NCBI nr
Match: KAG6576052.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 435.3 bits (1118), Expect = 4.7e-118
Identity = 222/320 (69.38%), Postives = 264/320 (82.50%), Query Frame = 0 

Query: 7   IEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPD 66
           IEE+PV+DI  QS ++SA SSL RAC E+GFFFIRNHG+SK+ Y+KLIS AD +LFS P+
Sbjct: 3   IEEIPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGAD-QLFSSPE 62

Query: 67  ETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDE 126
           ETKQKL +G SSYTPRFVVSP  ESFKV GPNFSASA A GFT SLFG QAT+F NLLDE
Sbjct: 63  ETKQKLTMGASSYTPRFVVSPFFESFKVSGPNFSASAAAQGFTQSLFGHQATEFSNLLDE 122

Query: 127 YGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLN-------- 186
           YGSKM+E+SKRV++LLL  LGD++E KFYDSEFS NCN YLRIN +TP+ +N        
Sbjct: 123 YGSKMKEVSKRVVKLLLNILGDNIEDKFYDSEFS-NCNAYLRINGFTPRSMNEEEEEEEE 182

Query: 187 DKKEVDALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDV--KPSEDGDLVVNIGDFMEA 246
           +++E++  KKHTD+SY+TI+FQ+DI GLQMR KGG+W DV  +PSED +L+VNIGDFMEA
Sbjct: 183 EEEELEVFKKHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPRPSEDDNLMVNIGDFMEA 242

Query: 247 WSNGRVKSAEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPIST 306
           WSNGR++SA HRVVLRKNN+K RFS+AFFLT EDDD KEIYAPSE+VGEG SRLY P ST
Sbjct: 243 WSNGRLRSAVHRVVLRKNNMK-RFSMAFFLTPEDDD-KEIYAPSEIVGEGKSRLYRPFST 302

Query: 307 QEYRLFREKNDERIESTLKD 317
           Q+YRLF+EK+D      LKD
Sbjct: 303 QQYRLFKEKSDVNARYALKD 318

[Close]
BLAST of Lsi02G022040 vs. NCBI nr
Match: KAG6576054.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 434.5 bits (1116), Expect = 8.0e-118
Identity = 214/314 (68.15%), Postives = 262/314 (83.44%), Query Frame = 0 

Query: 7   IEEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPD 66
           IEEVPV+DI SQS ++S  SSL RAC E GFFFI+NHG+SK+FY+KLIS  D +LFS P+
Sbjct: 3   IEEVPVIDISSQSLTESTASSLTRACTELGFFFIKNHGISKEFYQKLISGID-QLFSSPE 62

Query: 67  ETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQATQFRNLLDE 126
           ETKQKLK GVSSYTPRFVVSP +ESFK+ GP FSASAV HGFT S FG QAT+F NLLDE
Sbjct: 63  ETKQKLKQGVSSYTPRFVVSPFLESFKLSGPKFSASAVTHGFTQSQFGHQATEFSNLLDE 122

Query: 127 YGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDKKEVDAL 186
           YGSKM E+SK+V++LLLK LGD++E KFYD E+S NC  YLRIN YTP  +ND++EV+  
Sbjct: 123 YGSKMTEVSKKVVKLLLKILGDNIENKFYDPEYS-NCTSYLRINGYTPLGMNDEEEVEVF 182

Query: 187 KKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRVKSAE 246
           KKHTD+SY+TI+F +D+ GLQMRSKGG+W +V+PSEDG+LVVNIGD+MEAWSNGR++SA 
Sbjct: 183 KKHTDLSYVTIVFPNDMRGLQMRSKGGDWINVRPSEDGNLVVNIGDYMEAWSNGRLRSAL 242

Query: 247 HRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYRLFREKN 306
           HRVVLRK N+  RFS+A FL+  +D+++EIYAPSEVVGEG SRLY   S Q+Y+ FRE N
Sbjct: 243 HRVVLRK-NITKRFSIA-FLSTPEDESREIYAPSEVVGEGKSRLYRSFSIQQYKHFRENN 302

Query: 307 DERIESTLKDFAGI 321
            E++ +T+KDF GI
Sbjct: 303 YEKVGATIKDFVGI 312

[Close]
BLAST of Lsi02G022040 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.5e-77
Identity = 162/320 (50.62%), Postives = 216/320 (67.50%), Query Frame = 0 

Query: 9   EVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPDET 68
           ++PV+D+ +Q    S +SSL  ACKEWGFF++ NHG+SK+ + K+ S   R +F  P E+
Sbjct: 9   QLPVLDL-TQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICS-LSRDVFKAPLES 68

Query: 69  KQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLF-GPQATQFRNLLDEY 128
             KLK+G  SYTPR++ SP  ES  V GP+FS SA A    D LF      + R  + EY
Sbjct: 69  --KLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKAS--ADVLFQDHHKPELRETMQEY 128

Query: 129 GSKMQEISKRVIQ-LLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDKKE--VD 188
           G+KM E+SKR+I+ LL+ TLGD+   + Y ++FS NC+GYLR+ NYTP    +K+E  V+
Sbjct: 129 GAKMAELSKRLIKILLMMTLGDETGKRLYQTDFS-NCHGYLRLVNYTPPHDVEKQEELVE 188

Query: 189 ALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRVKS 248
            L  HTD+S +TI++QD +GGLQMRSK G+W D+ P  D  LVVNIGD M+AWSNGR++S
Sbjct: 189 GLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCND-FLVVNIGDLMQAWSNGRLRS 248

Query: 249 AEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYRLFRE 308
           +EHRVVLRK  + NR S+AFFL  ED+  K I AP E+VGEG  R Y      EY  FR+
Sbjct: 249 SEHRVVLRK--LVNRVSLAFFLCFEDE--KVILAPQEIVGEGKQRSYKSFKCSEYLKFRQ 308

Query: 309 KND----ERIESTLKDFAGI 321
            N+    E+I  T+KDFAG+
Sbjct: 309 SNEEGKFEKIGFTVKDFAGL 316

[Close]
BLAST of Lsi02G022040 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-70
Identity = 153/333 (45.95%), Postives = 219/333 (65.77%), Query Frame = 0 

Query: 9   EVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFS---FP 68
           E+PV DI S+  S+S+++SL+ ACKEWGFF++ NHGVS+D Y+KL      R FS   F 
Sbjct: 4   ELPVFDI-SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKL------RRFSTGVFE 63

Query: 69  DETKQKLKVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAHGFTDSLFGPQAT--QFRNL 128
            E ++K+K+G S+YTPRF+ SP  ES +V GP+F ASA +   +   F  QAT  +F  L
Sbjct: 64  LEDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGL 123

Query: 129 LDEYGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYT-PQDLNDKKE 188
           + EYG KM ++ +++++ +L + GDDL  K+Y+SEF  NC+GY RINNYT P D  D   
Sbjct: 124 MKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEF-GNCHGYFRINNYTIPSDQEDDHH 183

Query: 189 -------VDALKKHTDISYLTILFQDDIGGLQMRSKGG-EWFDVKPSEDGDLVVNIGDFM 248
                  ++ L  HTD+S +TI+ QDDIGGLQ+R++ G    D+ P ++  LVVN+GD +
Sbjct: 184 NGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEA-LVVNVGDLL 243

Query: 249 EAWSNGRVKSAEHRVVL-RKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVG--EGNSRLY 308
            AW+NGR++S++HRV+L R+  V NRFS+AFF     DD K ++AP EVVG  EG  R++
Sbjct: 244 HAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWC--FDDGKVVFAPDEVVGGCEG-MRVF 303

Query: 309 LPISTQEYRLFREKND----ERIESTLKDFAGI 321
                 +Y  FRE N+    E++  T++DFA I
Sbjct: 304 RSFKCGDYLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]
BLAST of Lsi02G022040 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 3.6e-52
Identity = 110/204 (53.92%), Postives = 145/204 (71.08%), Query Frame = 0 

Query: 124 LDEYGSKMQEISKRVIQ-LLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDKKE 183
           + EYG+KM E+SKR+I+ LL+ TLGD+   + Y ++FS NC+GYLR+ NYTP    +K+E
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFS-NCHGYLRLVNYTPPHDVEKQE 60

Query: 184 --VDALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNG 243
             V+ L  HTD+S +TI++QD +GGLQMRSK G+W D+ P  D  LVVNIGD M+AWSNG
Sbjct: 61  ELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCND-FLVVNIGDLMQAWSNG 120

Query: 244 RVKSAEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAPSEVVGEGNSRLYLPISTQEYR 303
           R++S+EHRVVLRK  + NR S+AFFL  ED+  K I AP E+VGEG  R Y      EY 
Sbjct: 121 RLRSSEHRVVLRK--LVNRVSLAFFLCFEDE--KVILAPQEIVGEGKQRSYKSFKCSEYL 180

Query: 304 LFREKND----ERIESTLKDFAGI 321
            FR+ N+    E+I  T+KDFAG+
Sbjct: 181 KFRQSNEEGKFEKIGFTVKDFAGL 198

[Close]
BLAST of Lsi02G022040 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.9e-29
Identity = 117/321 (36.45%), Postives = 162/321 (50.47%), Query Frame = 0 

Query: 8   EEVPVMDIDSQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFSFPDE 67
           E +PV+DI S    +S   ++  A +EWGFF + NHGVS +    +   A  R F  P E
Sbjct: 60  ESIPVIDI-SNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENM-KTATHRFFGLPVE 119

Query: 68  TKQKLKVGVS-SYTPRFVVSPSMESFKVL--GPNFSASAVAHGFTDSLFGPQATQFRNLL 127
            K+K     S S   RF  S S  + K L      S   V+      L+ P + +   L 
Sbjct: 120 EKRKFSREKSLSTNVRFGTSFSPHAEKALEWKDYLSLFFVSEAEASQLW-PDSCRSETL- 179

Query: 128 DEYGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRIN-NYTPQDLNDKKEV 187
            EY   M E +K +++ LL+ LG++L  K  D    +   G  RIN NY P   N +  V
Sbjct: 180 -EY---MNE-TKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPNPELTV 239

Query: 188 DALKKHTDISYLTILFQDDIGGLQMRS-KGGEWFDVKPSEDGDLVVNIGDFMEAWSNGRV 247
             + +H+D+S LTIL QD+IGGL +RS   G W  V P   G LV+NIGD M+  SNGR 
Sbjct: 240 -GVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI-SGSLVINIGDAMQIMSNGRY 299

Query: 248 KSAEHRVVLRKNNVKNRFSVAFFLTNEDDDNKEIYAP-SEVVGEGNSRLYLPISTQEY-- 307
           KS EHRV+   N   NR SV  F++ + +    +  P  EV+  G   +Y  I   +Y  
Sbjct: 300 KSVEHRVL--ANGSYNRISVPIFVSPKPE---SVIGPLLEVIENGEKPVYKDILYTDYVK 359

Query: 308 RLFREKNDERIESTLKDFAGI 321
             FR+ +D +   T+ DFA I
Sbjct: 360 HFFRKAHDGK--KTI-DFANI 361

[Close]
BLAST of Lsi02G022040 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 125.6 bits (314), Expect = 7.4e-29
Identity = 90/265 (33.96%), Postives = 136/265 (51.32%), Query Frame = 0 

Query: 9   EVPVMDID-----SQSFSQSAISSLRRACKEWGFFFIRNHGVSKDFYRKLISDADRRLFS 68
           E+PV+D+      ++   +    ++ RA +EWGFF + NHG+S D   K+  +   R+F 
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQE-QIRVFR 100

Query: 69  FPDETKQKL-KVGVSSYTPRFVVSPSMESFKVLGPNFSASAVAH-GFTDSLFGPQATQFR 128
            P + K K  K    SY      +PS  S + L    S S   H   TD       T   
Sbjct: 101 EPFDKKSKSEKFSAGSYR---WGTPSATSIRQL----SWSEAFHVPMTDISDNKDFTTLS 160

Query: 129 NLLDEYGSKMQEISKRVIQLLLKTLGDDLETKFYDSEFSNNCNGYLRINNYTPQDLNDKK 188
           + ++++ S+ + ++  + ++L +  G +  + F ++   N C  YLR+N Y P       
Sbjct: 161 STMEKFASESEALAYMLAEVLAEKSGQN-SSFFKENCVRNTC--YLRMNRYPP--CPKPS 220

Query: 189 EVDALKKHTDISYLTILFQDDIGGLQMRSKGGEWFDVKPSEDGDLVVNIGDFMEAWSNGR 248
           EV  L  HTD  +LTIL+QD +GGLQ+  K   W  VKP+    L++NIGD  +AWSNG 
Sbjct: 221 EVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKA-LIINIGDLFQAWSNGM 280

Query: 249 VKSAEHRVVLRKNNVKNRFSVAFFL 267
            KS EHRV+   N    RFS A+F+
Sbjct: 281 YKSVEHRVM--TNPKVERFSTAYFM 288

The following BLAST results are available for this feature:
BLAST of Lsi02G022040 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4KBY02.4e-6945.95Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310... [more]
Q9C8992.7e-2836.45Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S... [more]
O495611.0e-2733.96Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ... [more]
Q9C6I42.6e-2630.57Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ... [more]
Q9LHN85.7e-2633.01Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S... [more]
BLAST of Lsi02G022040 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JW964.1e-12071.15gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GMS44.6e-11969.59gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1GPF25.0e-11868.15gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145593... [more]
A0A6J1JSZ73.3e-11768.15gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488065 ... [more]
A0A6J1GMT13.1e-11567.52gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145593... [more]
BLAST of Lsi02G022040 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038899419.11.0e-14982.87gibberellin 20-oxidase-like protein [Benincasa hispida][more]
XP_022991453.18.5e-12071.15gibberellin 20-oxidase-like protein isoform X1 [Cucurbita maxima][more]
XP_022953356.19.4e-11969.59gibberellin 20-oxidase-like protein isoform X1 [Cucurbita moschata][more]
KAG6576052.14.7e-11869.38Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro... [more]
KAG6576054.18.0e-11868.15Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro... [more]
BLAST of Lsi02G022040 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G23340.11.5e-7750.632-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G51310.11.7e-7045.952-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G23340.23.6e-5253.922-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G55290.11.9e-2936.452-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G21200.17.4e-2933.96gibberellin 2-oxidase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 168..267
e-value: 2.1E-21
score: 76.3
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 10..73
e-value: 2.3E-13
score: 50.9
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..309
e-value: 1.1E-64
score: 220.9
NoneNo IPR availablePANTHERPTHR47990:SF9GIBBERELLIN 20-OXIDASE-LIKE PROTEINcoord: 6..309
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 6..309
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 8..304
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 163..268
score: 11.353998

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G022040.1Lsi02G022040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity