Lsi02G021330 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G021330
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptiontranscription factor bHLH62-like
Locationchr02: 27636934 .. 27639838 (+)
RNA-Seq ExpressionLsi02G021330
SyntenyLsi02G021330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAATCCTCTTTCTTTCTCCTTGTTTCATTTCCCTATTTATCTTCTTCTCACTCTCCAAATCCATCCCCTTCCACTTCCCATTCTCTCTGTGTGTTTCTTATATAAAAGTTTGCTTTTGATTCACTTTTAGCCTCCTCCAACCCATCAATCCCATTTCCCATTTCTTAATTGGGTGAAGCAACTTTTCTCTTGCGAAATTGTTGTTAATGGAGAACGAATTCTTCCTCAATTCTACTGGAATTTCTCAATCTCTTATGCATTTCGACTCCAATCCTTCCATGGCTACCTGGCATCATCCGTTTTCCTCCGCCATGGATGTTCCACCTCACTCTCAATCTCAACTTCAAGTTCCAGAGCTTAATCATTGTTCTTCCTCTCACCAATCCCCACCGGATTGTTTTCTCAATCCGGTTCCCGATCAGAGCTTTCAATTCGATTCTGCTCTTAGTTCCATGGTTTCTTCTCCAGCGGCCTCGAATTCTAACCTAACGAACGAGAGCTTCGTGATCAGAGAATTGATCGGGAAATTAGGTGGAAATTCTGAGAGGAGGATCTTGGAATTGAATTCTACTGCCGCATTGACTCCTTCTTCTTCCAGTGTGGCGGAATTTTCGTCCGATCCTGGATTTGCTGAGCGAGCTGCTAGGTTTTCGTGTTTTGGAAGTAGGAGCTTCAATGGCCGTCAATTGACGAATGAATTCGGTAATTATAGATCTCATCTCTCGATCACTAACGAGAAATTGAGCCGAGTTTCGAGTAGTCCGTCGCTTAAAGCACTTGGATCTGAAACGAATTTGCAAGAGCACAAGAATAATTCATCTTCGCAAGAAGATGAATCGTCTCTCTCGAATCCAGACAAAACGATTTCGAATCCACGGAAGAGAAAGGCAATTACAAAGCCTAAACTCAAAGAACCGGTAAAATTCCAACATCATCTCATCTCCTTTTCGTCTACATCTGAAATTGTGAAATCATTCCCTGAAAATTGAGTTTCGTTTTTGTTCCGCCATTGTTGCAGGTGGTTGAAGCTGCTCCCGAGAAGGGAAGTCCGAAGAAATTGAAGGCAGTGGAAACAAAGGAAAACGAAAAGGGGAACGTGAAAACGGAAGAGGATTTGAAGAAAACCGATGAAAATTTAGCGGAAGAGGAGAAGCAAACGAAGGCGAATTCCAAACCTCCTGAAGCTCCTAAGGATTACATTCACGTTAGAGCAAGAAGAGGCCAAGCAACTGATAGCCATAGTCTTGCTGAAAGAGTGAGTGAACTAATTTGTTTGTAGCTCTAATTTCAACACAAAATTAAATGATACTCATTCTGATTCTGATGATTATGGTGATGATTATTGATAATGATATTGATAATGTCTGTCTGTAAATCTCAATTTTCTGAATTCAAGGTTAGAAGAGAGAAAATCAGTGAAAGAATGAAGCTTCTTCAAGATCTTGTACCAGGACGCAACAAGGTTGGTCAGAAAACCAAAAAAAAAAAAAAAAAAACTATTTAGATGCCATTTTTTTTTTTTTTTTTTTTTTTCCTATTGGGGATATTAATTTTCATTAAAGAAAATTTGTTTTTGGTCAATTTTAGGTTACTGGAAAAGCTCTGATGTTGGATGAGATTATCAACTATGTGCAGTCATTACAACACCAAGTTGAGGTAATTATATTCTTTTAAAAATGGCCCACTTAAGTTTATAGGGATATAATTGAAACTTGAATTTGTAATTGAATGATTTTGAGTTGGGTTTCAGTTTCTGTCCATGAAGTTGGCTTCTGTGAATACAACCAGGGTGGATTTCAACATGGATTCTCTTATCTCAAAACAAGTAATTTTTTCTCCCCCTTTTCTTCTCTCACTCACCCAAAAAAAAACCCATGAAAAAGAATAAACAAAAACATTAAAAAAATAATAAAAGAAAGTGTTTTTCTTTTCTTTTTCCCTCACTTTTTCTTTCTTTACTCTTTGGTGAACTTCCATTTCACTTTATTATTATTGTTTTGTTTTTTTTTTTTTTCTTTAACAAATTTTTTTTGGGGTTGAGACAGACGTACCAATCAGGAACCAGTTTGACACATCCTCAAATTTCCCCTATAGATTCCTCAGCTTCAAGCTTTTATGGGCACCAAAATTCCTCTCTCCCAACCACCTCTCATTGCTCTGTGGACCCTCTTGACTCTGTTTTATGCCAAAATCTTCCCATTCAACTCCCTCCTCTCAATTCCTTCCTTCAAAATCCCTCTCAGGTAATTCAACGATTCCTTAAGATTACGGTTATTCTCGTCGTTGAATCCCGACTCAATTAAATTTTCTATTTTATGTATGACACAGTATCCAAATTTCGGAGAAGATGAACTTCAAAGCATTGTTCAAATGGGGTTCGTCCAAAATCAGACCCAAGAAATATCATTGCAATCTCACAATTTCAACTTAGGTAATCATACATTGTAATAATTAATAATAATAATAGTAACTTCAAGATGTGATTAATAAATTTAATTTTGAAAATTTAAAACTGAAAGTGGAATTTTGTGATGATGCAGGCTCAGACCAAATGAAAATTGAACTCTAATCTCTTCCTTCAACACCATACACAAAGGCTGCTGTTGTAAATACAAGAGATTAATTTTCAAGCATTGATTAAAAGAAGAAAAAAAAAATCCCATTTATTCCATTTCTTAGGTCTAATTAAGGAGAGAGAAAATCAGTTTGTCCTTTTTTTTTCTTCCAATGGGTAATCTTTGTTTTATATTTTAATATTGTTGTTTTTTTTTTTCCTTCTAATTTTGTGGGATGGGAGAGGAAAGTTTCTACTTTTCTTATAACTTTCCACTGTAAAATCTCTAGAGCAAGTCTGTCCCTTTGGTGCAAAGTTGTACAGCACCACAATGTAAGATAAAAAGCC

mRNA sequence

AAACAATCCTCTTTCTTTCTCCTTGTTTCATTTCCCTATTTATCTTCTTCTCACTCTCCAAATCCATCCCCTTCCACTTCCCATTCTCTCTGTGTGTTTCTTATATAAAAGTTTGCTTTTGATTCACTTTTAGCCTCCTCCAACCCATCAATCCCATTTCCCATTTCTTAATTGGGTGAAGCAACTTTTCTCTTGCGAAATTGTTGTTAATGGAGAACGAATTCTTCCTCAATTCTACTGGAATTTCTCAATCTCTTATGCATTTCGACTCCAATCCTTCCATGGCTACCTGGCATCATCCGTTTTCCTCCGCCATGGATGTTCCACCTCACTCTCAATCTCAACTTCAAGTTCCAGAGCTTAATCATTGTTCTTCCTCTCACCAATCCCCACCGGATTGTTTTCTCAATCCGGTTCCCGATCAGAGCTTTCAATTCGATTCTGCTCTTAGTTCCATGGTTTCTTCTCCAGCGGCCTCGAATTCTAACCTAACGAACGAGAGCTTCGTGATCAGAGAATTGATCGGGAAATTAGGTGGAAATTCTGAGAGGAGGATCTTGGAATTGAATTCTACTGCCGCATTGACTCCTTCTTCTTCCAGTGTGGCGGAATTTTCGTCCGATCCTGGATTTGCTGAGCGAGCTGCTAGGTTTTCGTGTTTTGGAAGTAGGAGCTTCAATGGCCGTCAATTGACGAATGAATTCGGTAATTATAGATCTCATCTCTCGATCACTAACGAGAAATTGAGCCGAGTTTCGAGTAGTCCGTCGCTTAAAGCACTTGGATCTGAAACGAATTTGCAAGAGCACAAGAATAATTCATCTTCGCAAGAAGATGAATCGTCTCTCTCGAATCCAGACAAAACGATTTCGAATCCACGGAAGAGAAAGGCAATTACAAAGCCTAAACTCAAAGAACCGGTGGTTGAAGCTGCTCCCGAGAAGGGAAGTCCGAAGAAATTGAAGGCAGTGGAAACAAAGGAAAACGAAAAGGGGAACGTGAAAACGGAAGAGGATTTGAAGAAAACCGATGAAAATTTAGCGGAAGAGGAGAAGCAAACGAAGGCGAATTCCAAACCTCCTGAAGCTCCTAAGGATTACATTCACGTTAGAGCAAGAAGAGGCCAAGCAACTGATAGCCATAGTCTTGCTGAAAGAGTTAGAAGAGAGAAAATCAGTGAAAGAATGAAGCTTCTTCAAGATCTTGTACCAGGACGCAACAAGGTTACTGGAAAAGCTCTGATGTTGGATGAGATTATCAACTATGTGCAGTCATTACAACACCAAGTTGAGTTTCTGTCCATGAAGTTGGCTTCTGTGAATACAACCAGGGTGGATTTCAACATGGATTCTCTTATCTCAAAACAAACGTACCAATCAGGAACCAGTTTGACACATCCTCAAATTTCCCCTATAGATTCCTCAGCTTCAAGCTTTTATGGGCACCAAAATTCCTCTCTCCCAACCACCTCTCATTGCTCTGTGGACCCTCTTGACTCTGTTTTATGCCAAAATCTTCCCATTCAACTCCCTCCTCTCAATTCCTTCCTTCAAAATCCCTCTCAGTATCCAAATTTCGGAGAAGATGAACTTCAAAGCATTGTTCAAATGGGGTTCGTCCAAAATCAGACCCAAGAAATATCATTGCAATCTCACAATTTCAACTTAGGCTCAGACCAAATGAAAATTGAACTCTAATCTCTTCCTTCAACACCATACACAAAGGCTGCTGTTGTAAATACAAGAGATTAATTTTCAAGCATTGATTAAAAGAAGAAAAAAAAAATCCCATTTATTCCATTTCTTAGGTCTAATTAAGGAGAGAGAAAATCAGTTTGTCCTTTTTTTTTCTTCCAATGGGTAATCTTTGTTTTATATTTTAATATTGTTGTTTTTTTTTTTCCTTCTAATTTTGTGGGATGGGAGAGGAAAGTTTCTACTTTTCTTATAACTTTCCACTGTAAAATCTCTAGAGCAAGTCTGTCCCTTTGGTGCAAAGTTGTACAGCACCACAATGTAAGATAAAAAGCC

Coding sequence (CDS)

ATGGAGAACGAATTCTTCCTCAATTCTACTGGAATTTCTCAATCTCTTATGCATTTCGACTCCAATCCTTCCATGGCTACCTGGCATCATCCGTTTTCCTCCGCCATGGATGTTCCACCTCACTCTCAATCTCAACTTCAAGTTCCAGAGCTTAATCATTGTTCTTCCTCTCACCAATCCCCACCGGATTGTTTTCTCAATCCGGTTCCCGATCAGAGCTTTCAATTCGATTCTGCTCTTAGTTCCATGGTTTCTTCTCCAGCGGCCTCGAATTCTAACCTAACGAACGAGAGCTTCGTGATCAGAGAATTGATCGGGAAATTAGGTGGAAATTCTGAGAGGAGGATCTTGGAATTGAATTCTACTGCCGCATTGACTCCTTCTTCTTCCAGTGTGGCGGAATTTTCGTCCGATCCTGGATTTGCTGAGCGAGCTGCTAGGTTTTCGTGTTTTGGAAGTAGGAGCTTCAATGGCCGTCAATTGACGAATGAATTCGGTAATTATAGATCTCATCTCTCGATCACTAACGAGAAATTGAGCCGAGTTTCGAGTAGTCCGTCGCTTAAAGCACTTGGATCTGAAACGAATTTGCAAGAGCACAAGAATAATTCATCTTCGCAAGAAGATGAATCGTCTCTCTCGAATCCAGACAAAACGATTTCGAATCCACGGAAGAGAAAGGCAATTACAAAGCCTAAACTCAAAGAACCGGTGGTTGAAGCTGCTCCCGAGAAGGGAAGTCCGAAGAAATTGAAGGCAGTGGAAACAAAGGAAAACGAAAAGGGGAACGTGAAAACGGAAGAGGATTTGAAGAAAACCGATGAAAATTTAGCGGAAGAGGAGAAGCAAACGAAGGCGAATTCCAAACCTCCTGAAGCTCCTAAGGATTACATTCACGTTAGAGCAAGAAGAGGCCAAGCAACTGATAGCCATAGTCTTGCTGAAAGAGTTAGAAGAGAGAAAATCAGTGAAAGAATGAAGCTTCTTCAAGATCTTGTACCAGGACGCAACAAGGTTACTGGAAAAGCTCTGATGTTGGATGAGATTATCAACTATGTGCAGTCATTACAACACCAAGTTGAGTTTCTGTCCATGAAGTTGGCTTCTGTGAATACAACCAGGGTGGATTTCAACATGGATTCTCTTATCTCAAAACAAACGTACCAATCAGGAACCAGTTTGACACATCCTCAAATTTCCCCTATAGATTCCTCAGCTTCAAGCTTTTATGGGCACCAAAATTCCTCTCTCCCAACCACCTCTCATTGCTCTGTGGACCCTCTTGACTCTGTTTTATGCCAAAATCTTCCCATTCAACTCCCTCCTCTCAATTCCTTCCTTCAAAATCCCTCTCAGTATCCAAATTTCGGAGAAGATGAACTTCAAAGCATTGTTCAAATGGGGTTCGTCCAAAATCAGACCCAAGAAATATCATTGCAATCTCACAATTTCAACTTAGGCTCAGACCAAATGAAAATTGAACTCTAA

Protein sequence

MENEFFLNSTGISQSLMHFDSNPSMATWHHPFSSAMDVPPHSQSQLQVPELNHCSSSHQSPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILELNSTAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEKLSRVSSSPSLKALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPVVEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQHQVEFLSMKLASVNTTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSSLPTTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQEISLQSHNFNLGSDQMKIEL
Homology
BLAST of Lsi02G021330 vs. ExPASy Swiss-Prot
Match: Q9FJL4 (Transcription factor bHLH78 OS=Arabidopsis thaliana OX=3702 GN=BHLH78 PE=1 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 2.3e-74
Identity = 243/558 (43.55%), Postives = 301/558 (53.94%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMAT-WHHPFSSA---------MDVPPHSQSQLQVPE 60
           M+NE F+N+         F   P MAT + H  SS+         MD  PH  S      
Sbjct: 1   MDNELFMNT--------EFPPPPEMATHFEHQQSSSSAMMLNWALMDPNPHQDSSF---- 60

Query: 61  LNHCSSSHQSPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLT---NESFVIRELIGK 120
           L   S+  Q            Q   FDSALSS+VSSP  SNSN +    + F+IRELIGK
Sbjct: 61  LWEKSTEQQ-----------QQQSIFDSALSSLVSSPTPSNSNFSGGGGDGFLIRELIGK 120

Query: 121 LG--GNSERRILEL-------NSTAALTPSSS-----------------SVAEFSSDPGF 180
           LG  GN+     E+       +++   TP SS                  + EFS+DPGF
Sbjct: 121 LGNIGNNNNNSGEIYGTPMSRSASCYATPMSSPPPPTNSNSQMMMNRTTPLTEFSADPGF 180

Query: 181 AERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEKLSRVSSSPSLKALGSETNLQEHK 240
           AERAARFSCFGSRSFNGR  TN   N  +++   + KL+RVSS+P+LKAL S        
Sbjct: 181 AERAARFSCFGSRSFNGRTNTNLPINNGNNMVNNSGKLTRVSSTPALKALVS-------- 240

Query: 241 NNSSSQEDESSLSNPDKTISN--PRKRKAITKPKLKE-PVVEAAPEKGSPKKLKAVETKE 300
                         P+ T      RKRK++ K K KE P+  A+P   SP   K  E K 
Sbjct: 241 --------------PEVTPGGEFSRKRKSVPKGKSKENPISTASP---SPSFSKTAE-KN 300

Query: 301 NEKGNVKTEED----LKKTDENLAEEEKQTKA----NSKPPEAPKDYIHVRARRGQATDS 360
             KG  K+ E+     ++ +E+  EEE + +     N+KPPE PKDYIHVRARRGQATDS
Sbjct: 301 GGKGGSKSSEEKGGKRRREEEDDEEEEGEGEGNKSNNTKPPEPPKDYIHVRARRGQATDS 360

Query: 361 HSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQHQVEFLSMKLASV 420
           HSLAERVRREKI ERMKLLQDLVPG NKVTGKALMLDEIINYVQSLQ QVEFLSMKL+SV
Sbjct: 361 HSLAERVRREKIGERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQRQVEFLSMKLSSV 420

Query: 421 NTTRVDFNMDSLISKQT-YQSGTSLTHPQISPIDSSAS------SFYGHQNSSLPTTSHC 480
           N TR+DFN+D+L+SK     S  +  H +     SS+       + Y + +  LP  S  
Sbjct: 421 NDTRLDFNVDALVSKDVMIPSSNNRLHEEGLQSKSSSHHHQQQLNIYNNNSQLLPNISSN 480

Query: 481 SV---DPLDSVLCQNLP---IQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQEI 496
           ++    P++S+    L      LP L  F  + SQY  F E++LQSIV MG  +N   E 
Sbjct: 481 NMMLQSPMNSLETSTLARSFTHLPTLTQFTDSISQYQMFSEEDLQSIVGMGVAENPNNE- 498

BLAST of Lsi02G021330 vs. ExPASy Swiss-Prot
Match: Q9SRT2 (Transcription factor bHLH62 OS=Arabidopsis thaliana OX=3702 GN=BHLH62 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.4e-71
Identity = 224/509 (44.01%), Postives = 274/509 (53.83%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPFSSAMDVPPHSQSQLQVPELNHCSSSHQS 60
           MENE F+N+ G+S                HP    M  P  S + L+   +         
Sbjct: 1   MENELFMNA-GVS----------------HP--PVMTSPSSSSAMLKWVSMETQPVDPSL 60

Query: 61  PPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLT-----NESFVIRELIGKLG--GNSE 120
             + F     +QS  FDSALSS+VSSP  SNSN +      E+ ++RELIGKLG  G+  
Sbjct: 61  SRNLFWEKSTEQSI-FDSALSSLVSSPTPSNSNFSVGGVGGENVIMRELIGKLGNIGDIY 120

Query: 121 RRILELNSTAALTPSSS-------------SVAEFSSDPGFAERAARFSCFGSRSFNGRQ 180
                  ++   TP SS              +AE S DPGFAERAARFSCFGSRSFN R 
Sbjct: 121 GITASNGNSCYATPMSSPPPGSMMETKTTTPMAELSGDPGFAERAARFSCFGSRSFNSR- 180

Query: 181 LTNEFGNYRSHLSITNEKLSRVSSSPSLKALGSETNLQEHKNNSSSQEDESSLSNPDKTI 240
             + F         TNEK+ RVSSSP  K L S            + E    LS   KT 
Sbjct: 181 TNSPFPINNEPPITTNEKMPRVSSSPVFKPLASHV---------PAGESSGELSRKRKTK 240

Query: 241 SNPRKRKAITKPKLKEPVVEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEE 300
           S      A++  K  E       E   PK+ K            K+EE+  KT       
Sbjct: 241 SKQNSPSAVSSSKEIE-----EKEDSDPKRCK------------KSEENGDKT------- 300

Query: 301 EKQTKANSKPPEAPKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVT 360
                   K  +  KDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVT
Sbjct: 301 --------KSIDPYKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVT 360

Query: 361 GKALMLDEIINYVQSLQHQVEFLSMKLASVNTTRVDFNMDSLISKQTYQSGTSLT-HPQI 420
           GKALMLDEIINYVQSLQ QVEFLSMKL+SVN TR+DFNMD+L+SK  + S  +L  H Q+
Sbjct: 361 GKALMLDEIINYVQSLQRQVEFLSMKLSSVN-TRLDFNMDALLSKDIFPSSNNLMHHQQV 420

Query: 421 SPIDSSASSFYG-HQNSSL----PTTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYP 480
             +DSSA +  G H N +L      +S+  ++PL++   ++    LP L  F  + SQY 
Sbjct: 421 LQLDSSAETLLGDHHNKNLQLNPDISSNNVINPLETSETRSFISHLPTLAHFTDSISQYS 446

Query: 481 NFGEDELQSIVQMGFVQNQTQEISLQSHN 484
            F ED+L SI+ MGF QN+ QE++  S N
Sbjct: 481 TFSEDDLHSIIHMGFAQNRLQELNQGSSN 446

BLAST of Lsi02G021330 vs. ExPASy Swiss-Prot
Match: Q9LK48 (Transcription factor bHLH77 OS=Arabidopsis thaliana OX=3702 GN=BHLH77 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 5.7e-41
Identity = 142/319 (44.51%), Postives = 186/319 (58.31%), Query Frame = 0

Query: 189 KALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPVVEAAPEKGSP 248
           +  GS+   QE  N SS  + +S       T SN RKRK+I     KE     +P   S 
Sbjct: 81  REFGSQPTTQE-SNKSSLLDPDSVSDRVHTTKSNSRKRKSIPSGNGKE-----SPASSSL 140

Query: 249 KKLKAVETKEN--EKGNVKTEEDLKKTDENLAEE---EKQTKANSKPPEAPKDYIHVRAR 308
               +  + EN   KG  ++++D+  + +N  E+   +   K ++KPPEAPKDYIHVRAR
Sbjct: 141 TASNSKVSGENGGSKGGKRSKQDVAGSSKNGVEKCDSKGDNKDDAKPPEAPKDYIHVRAR 200

Query: 309 RGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQHQVEFL 368
           RGQATDSHSLAER RREKISERM LLQDLVPG N++TGKA+MLDEIINYVQSLQ QVEFL
Sbjct: 201 RGQATDSHSLAERARREKISERMTLLQDLVPGCNRITGKAVMLDEIINYVQSLQRQVEFL 260

Query: 369 SMKLASVNTTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSSLPTTSHC 428
           SMKLA+VN  R++FN ++ +S +  Q G SLT            S Y            C
Sbjct: 261 SMKLATVN-PRMEFNANASLSTEMIQPGESLT-----------QSLY---------AMAC 320

Query: 429 SVDPLDSV---LCQNLP----IQLPPLNSFLQNPSQYPNFGE-DELQSIVQMGFVQNQTQ 488
           S   L S    L +N+P     Q P  + F+   ++ P F E ++LQSIVQMGF     Q
Sbjct: 321 SEQRLPSAYYSLGKNMPRFSDTQFPSNDGFVH--TETPGFWENNDLQSIVQMGFGDILQQ 370

Query: 489 EISLQSHNFNLGSDQMKIE 495
           + +  ++N +  + QMK+E
Sbjct: 381 QSNNNNNNCSEPTLQMKLE 370

BLAST of Lsi02G021330 vs. ExPASy Swiss-Prot
Match: Q9CAA9 (Transcription factor bHLH49 OS=Arabidopsis thaliana OX=3702 GN=BHLH49 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.3e-34
Identity = 146/398 (36.68%), Postives = 208/398 (52.26%), Query Frame = 0

Query: 120 NSTAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFN---GRQLTNE------------ 179
           N+++ L P   S+A+F +D GF ERAARFS F   +F+    + L N             
Sbjct: 114 NASSFLPP---SMAQFPADSGFIERAARFSLFSGGNFSDMVNQPLGNSEAIGLFLQGGGT 173

Query: 180 -FGNYRSHLSITNEKLSRVSSSPSLKALGSETNLQEHKNNSS--SQEDESSLSNPDK--- 239
             G  +S+     E  + VS +     + S    + +   S   S++ +SS  N  K   
Sbjct: 174 MQGQCQSNELNVGEPHNDVSVAVKESTVRSSEQAKPNVPGSGNVSEDTQSSGGNGQKGRE 233

Query: 240 TISNPRKRKAITKPKLKEPVVEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLA 299
           T SN +KRK     +  +   EAA    S +  +  +   +EK N   E+      +   
Sbjct: 234 TSSNTKKRK-----RNGQKNSEAAQSHRSQQSEEEPDNNGDEKRN--DEQSPNSPGKKSN 293

Query: 300 EEEKQTKANSKPPEAPKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNK 359
             ++Q K +S PP+    YIHVRARRGQAT+SHSLAERVRREKISERMK LQDLVPG NK
Sbjct: 294 SGKQQGKQSSDPPK--DGYIHVRARRGQATNSHSLAERVRREKISERMKFLQDLVPGCNK 353

Query: 360 VTGKALMLDEIINYVQSLQHQVEFLSMKLASVNTTRVDFNMDSLISKQTYQ--SGTSLTH 419
           VTGKA+MLDEIINYVQSLQ QVEFLSMKLA+VN  ++DFN++ L++K   Q  +G+S T 
Sbjct: 354 VTGKAVMLDEIINYVQSLQRQVEFLSMKLATVN-PQMDFNLEGLLAKDALQLRAGSSSTT 413

Query: 420 PQISPIDSSASSFYGHQNSSLPTTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYPNF 479
           P           F  + + + P   H  +    S + + +   L P+N   +   Q  N 
Sbjct: 414 P-----------FPPNMSMAYPPLPHGFMQQTLSSIGRTITSPLSPMNGGFKR--QETNG 473

Query: 480 GEDELQSIVQMGFVQNQTQEISLQSHNFNLGSDQMKIE 495
            E +LQ+++ + +           +   +L +  MK+E
Sbjct: 474 WEGDLQNVIHINYGAGDVTPDPQAAATASLPAANMKVE 485

BLAST of Lsi02G021330 vs. ExPASy Swiss-Prot
Match: Q9C670 (Transcription factor bHLH76 OS=Arabidopsis thaliana OX=3702 GN=BHLH76 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.1e-31
Identity = 111/279 (39.78%), Postives = 159/279 (56.99%), Query Frame = 0

Query: 131 SVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRS-HLSITNEKLSRVSSSPSLK 190
           ++A+F +D GF ERAA+FS FG       Q  +  G   S  L + + ++   S   +  
Sbjct: 73  NMAQFPADSGFIERAAKFSFFGCGEMMMNQQQSSLGVPDSTGLFLQDTQIPSGSKLDNGP 132

Query: 191 ALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPVVEAAPEKGSPK 250
              +   ++E   N+ S++ +SS  N                        +A   + S K
Sbjct: 133 LTDASKLVKERSINNVSEDSQSSGGNGHD---------------------DAKCGQTSSK 192

Query: 251 KLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKD-YIHVRARRGQAT 310
              + + K   K   + E+  +K +++      +T +  +P ++ KD YIH+RARRGQAT
Sbjct: 193 GFSSKKRKRIGKDCEEEEDKKQKDEQSPTSNANKTNSEKQPSDSLKDGYIHMRARRGQAT 252

Query: 311 DSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQHQVEFLSMKLA 370
           +SHSLAERVRREKISERMK LQDLVPG +KVTGKA+MLDEIINYVQSLQ Q+EFLSMKL+
Sbjct: 253 NSHSLAERVRREKISERMKFLQDLVPGCDKVTGKAVMLDEIINYVQSLQCQIEFLSMKLS 312

Query: 371 SVNTTRVDFNMDSLISKQTYQSGT-------SLTHPQIS 401
           +VN   +DFN++SL++K   QS         S+ +P +S
Sbjct: 313 AVNPV-LDFNLESLLAKDALQSSAPTFPHNMSMLYPPVS 329

BLAST of Lsi02G021330 vs. ExPASy TrEMBL
Match: A0A0A0K8F4 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G360030 PE=4 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 4.7e-232
Identity = 451/500 (90.20%), Postives = 462/500 (92.40%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPFSSAMDVPPHSQSQLQVPELNHC-SSSHQ 60
           M+N+FFLN TGISQSLMHFDSNPS+ATWH  FSS MD+P       Q+P+L HC SSSHQ
Sbjct: 1   MDNQFFLNPTGISQSLMHFDSNPSIATWHQQFSSPMDLP------TQLPDLTHCSSSSHQ 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDCFLNPV DQSFQFDSALSSMVSSPAASNSN+TNESF IRELIGKLGGNSERRILEL
Sbjct: 61  SPPDCFLNPVSDQSFQFDSALSSMVSSPAASNSNITNESFAIRELIGKLGGNSERRILEL 120

Query: 121 -NST--AALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITN 180
            NST  AAL PSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSI N
Sbjct: 121 SNSTAAAALPPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSIGN 180

Query: 181 EKLSRVSSSPSLKALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKE 240
           EKLSRVSSSPSLKALGSE NLQEHKNNSSSQEDESSLSN DKTI+NPRKRKAITK KLKE
Sbjct: 181 EKLSRVSSSPSLKALGSEMNLQEHKNNSSSQEDESSLSNQDKTITNPRKRKAITKAKLKE 240

Query: 241 PVVEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKD 300
           PVVEA PEK SPKKLK VE KE    NVKTEEDLKK DEN A EE+QTKANSKPPEAPKD
Sbjct: 241 PVVEATPEKESPKKLKTVERKE----NVKTEEDLKKNDENSA-EERQTKANSKPPEAPKD 300

Query: 301 YIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSL 360
           YIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVTGKALMLDEIINYVQSL
Sbjct: 301 YIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSL 360

Query: 361 QHQVEFLSMKLASVNTTRVDFNMDSLI-SKQTYQSGTSLTHPQISPIDSSASSFYGHQNS 420
           QHQVEFLSMKLASVNTTRVDFN+DSLI SKQ YQSGTSLTHPQISPIDSS SSFYGHQNS
Sbjct: 361 QHQVEFLSMKLASVNTTRVDFNVDSLISSKQMYQSGTSLTHPQISPIDSSTSSFYGHQNS 420

Query: 421 SLPTTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQ 480
           SLPTTSHCSVDP+DSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQ
Sbjct: 421 SLPTTSHCSVDPIDSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQ 480

Query: 481 EISLQSHNFNLGSDQMKIEL 496
           EISLQSHNFNLGSDQMKIEL
Sbjct: 481 EISLQSHNFNLGSDQMKIEL 489

BLAST of Lsi02G021330 vs. ExPASy TrEMBL
Match: A0A6J1GRP7 (transcription factor bHLH62-like OS=Cucurbita moschata OX=3662 GN=LOC111456512 PE=4 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 8.7e-210
Identity = 423/502 (84.26%), Postives = 444/502 (88.45%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPF-SSAMDVPPHSQSQLQVPELNHCSSSHQ 60
           MEN+FFLN+TGI QSLMHFDSNPSMATWHH F SSAMDVPP    Q  +P+L HCSSSHQ
Sbjct: 1   MENQFFLNATGIPQSLMHFDSNPSMATWHHQFSSSAMDVPP----QPHLPDLTHCSSSHQ 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDCFLN VPDQSFQFDSALSSMVSSPAASNSNLTNESFVIR+LIGKLGGNSE RILEL
Sbjct: 61  SPPDCFLNSVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRQLIGKLGGNSEGRILEL 120

Query: 121 NSTAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEKL 180
           NSTAALTP SSSVAEF+SDPG AERAARFSCFGSRSFN RQLTNEFGNYRSHL I  EKL
Sbjct: 121 NSTAALTP-SSSVAEFTSDPGLAERAARFSCFGSRSFNDRQLTNEFGNYRSHLPIATEKL 180

Query: 181 SRVSSSPSLKALGSETN-LQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPV 240
           SRVSSSPS+KALGSETN L++HKNNSSS EDESSLSNPDK I N RKRKAITK KLKEP+
Sbjct: 181 SRVSSSPSIKALGSETNLLEQHKNNSSSHEDESSLSNPDKAIPNSRKRKAITKAKLKEPI 240

Query: 241 VEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKDYI 300
           VE APEK  PKKLKAVE KENEKGN+KTEED +K DE  A EEKQTK NSKPPE PKDYI
Sbjct: 241 VE-APEKEIPKKLKAVEPKENEKGNMKTEEDSQKIDEKTA-EEKQTKENSKPPEPPKDYI 300

Query: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQH 360
           HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVTGKALMLDEIINYVQSLQH
Sbjct: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQH 360

Query: 361 QVEFLSMKLASVN--TTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSS 420
           QVEFLSMKLASVN  TTRVDFN+DSLISKQ Y+SGTSL HPQISPI+SS S FYGH N S
Sbjct: 361 QVEFLSMKLASVNTTTTRVDFNVDSLISKQMYESGTSLRHPQISPIESSGSRFYGHTNPS 420

Query: 421 LPTTSHCSVDPLDSVLCQNLPIQ-LPPLN-SFLQN-PSQYPNFGEDELQSIVQMGFVQNQ 480
           LPTTSHCSVDPLDSVL QNLPIQ  PPLN SF QN PSQY +FGEDELQS+VQMGFVQNQ
Sbjct: 421 LPTTSHCSVDPLDSVLRQNLPIQHPPPLNSSFPQNHPSQYRSFGEDELQSMVQMGFVQNQ 480

Query: 481 TQEISLQSHNFNLGSDQMKIEL 496
           TQ  ++ +H    GS+QMKIEL
Sbjct: 481 TQNFNIGNH---AGSEQMKIEL 492

BLAST of Lsi02G021330 vs. ExPASy TrEMBL
Match: A0A6J1JX20 (transcription factor bHLH62-like OS=Cucurbita maxima OX=3661 GN=LOC111488239 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 9.0e-207
Identity = 419/502 (83.47%), Postives = 441/502 (87.85%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPF-SSAMDVPPHSQSQLQVPELNHCSSSHQ 60
           MENEFFLN+TGI QSLMHFDSNPS+ATWHH F SSAMDVPP    Q  +P+L HCSSSHQ
Sbjct: 1   MENEFFLNATGIPQSLMHFDSNPSLATWHHQFSSSAMDVPP----QPHLPDLTHCSSSHQ 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDCFL+ +PDQSFQFDSALSSMVSSPAASNSNLTNESFVIR+LIGKLGGNSERRILEL
Sbjct: 61  SPPDCFLSSIPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRQLIGKLGGNSERRILEL 120

Query: 121 NSTAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEKL 180
           NSTAALTP SSSVAEF+SDPG AERAARFSCFGSRSFN RQLTNEFGNYRSHL I  EKL
Sbjct: 121 NSTAALTP-SSSVAEFTSDPGLAERAARFSCFGSRSFNDRQLTNEFGNYRSHLPIATEKL 180

Query: 181 SRVSSSPSLKALGSETN-LQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPV 240
           SRVSSSPS+KALGSETN L++HKNNSSS EDESSLSNPDK I NPRKRKAITK KLKE +
Sbjct: 181 SRVSSSPSIKALGSETNLLEQHKNNSSSHEDESSLSNPDKAIPNPRKRKAITKAKLKELI 240

Query: 241 VEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKDYI 300
           VE APEK  PKK KAVE KENEKGN+KTEED  K DE  A EEKQTK NSKPPE PKDYI
Sbjct: 241 VE-APEKEIPKKPKAVEPKENEKGNMKTEEDSPKIDEKTA-EEKQTKENSKPPEPPKDYI 300

Query: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQH 360
           HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVTGKALMLDEIINYVQSLQH
Sbjct: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQH 360

Query: 361 QVEFLSMKLASVN--TTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSS 420
           QVEFLSMKLASVN  TTRVDFN+DSLISKQ Y+SGTSL HPQISPI+SS S FYGH N S
Sbjct: 361 QVEFLSMKLASVNTTTTRVDFNVDSLISKQMYESGTSLRHPQISPIESSGSRFYGHTNPS 420

Query: 421 LPTTSHCSVDPLDSVLCQNLPIQ-LPPLN-SFLQN-PSQYPNFGEDELQSIVQMGFVQNQ 480
           LPTTS CSVDPLDSVL QNLPIQ  PPLN SF QN P QY +FGEDELQS+VQMGFVQNQ
Sbjct: 421 LPTTSPCSVDPLDSVLRQNLPIQHPPPLNSSFPQNHPLQYRSFGEDELQSMVQMGFVQNQ 480

Query: 481 TQEISLQSHNFNLGSDQMKIEL 496
           TQ  ++ +H    GS+QMKIEL
Sbjct: 481 TQNFNIGNH---AGSEQMKIEL 492

BLAST of Lsi02G021330 vs. ExPASy TrEMBL
Match: A0A1S3C6Z0 (transcription factor bHLH78 OS=Cucumis melo OX=3656 GN=LOC103497607 PE=4 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 1.6e-203
Identity = 411/501 (82.04%), Postives = 426/501 (85.03%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPFSSAMDVPPHSQSQLQVPELNHC-SSSHQ 60
           M+N+FFLN TGISQSLMHFDSNPS+ATWH  FSSAMDVP       Q+P+L HC SSSHQ
Sbjct: 1   MDNQFFLNPTGISQSLMHFDSNPSIATWHQQFSSAMDVP------TQLPDLTHCSSSSHQ 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSN+TNESF IRELIGKLGGNSERRILEL
Sbjct: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNITNESFAIRELIGKLGGNSERRILEL 120

Query: 121 NST---AALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITN 180
           N++   AALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSI N
Sbjct: 121 NNSTAAAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSIGN 180

Query: 181 EKLSRVSSSPSLKALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKE 240
           EKLSRVSSSPSLKALGSE  LQEHKNNSSSQEDESSLSN DKTI+NPRKRKAITK KLKE
Sbjct: 181 EKLSRVSSSPSLKALGSEMKLQEHKNNSSSQEDESSLSNQDKTITNPRKRKAITKAKLKE 240

Query: 241 PVVEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKD 300
            VVEA PEK SPKKLK VETKE    NVKTEEDLKK DEN A EEKQ KANSKPPEAPKD
Sbjct: 241 SVVEATPEKESPKKLKTVETKE----NVKTEEDLKKNDENSA-EEKQAKANSKPPEAPKD 300

Query: 301 YIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIIN-YVQS 360
           YIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKV  +        N Y+  
Sbjct: 301 YIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVGQEHTHTHTHTNLYIYK 360

Query: 361 LQHQVEFLSMKLASVNTTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNS 420
                 FL +                   +Q YQSGTSLTHPQISPIDSSASSFYGHQNS
Sbjct: 361 F-----FLGL-------------------RQMYQSGTSLTHPQISPIDSSASSFYGHQNS 420

Query: 421 SLPTTSHCSVDPLDSVLCQNLP-IQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQT 480
           SLPTTSHCSVDP+DSVLCQNLP IQLPPLNSFLQNPSQ+PNFGEDELQSIVQMGFVQNQT
Sbjct: 421 SLPTTSHCSVDPIDSVLCQNLPTIQLPPLNSFLQNPSQFPNFGEDELQSIVQMGFVQNQT 466

Query: 481 QEISLQSHNFNLGSDQMKIEL 496
           QEISLQSHNFNLGSDQMKIEL
Sbjct: 481 QEISLQSHNFNLGSDQMKIEL 466

BLAST of Lsi02G021330 vs. ExPASy TrEMBL
Match: A0A6J1KVI5 (transcription factor bHLH62-like OS=Cucurbita maxima OX=3661 GN=LOC111497970 PE=4 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.9e-196
Identity = 397/477 (83.23%), Postives = 413/477 (86.58%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPFSSAMDVPPHSQSQLQVPEL-NHCSSSHQ 60
           MENEFFLN+T I QSLMHFDSNPSMATWHH FSSAMDV P  Q QL VPE  NHCSSSH 
Sbjct: 1   MENEFFLNATEIPQSLMHFDSNPSMATWHHSFSSAMDVQP-QQLQLHVPEFNNHCSSSHP 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDC+LNPVP QSFQFDSALSSMVSSPA SNSN TN+SFVIRELIGKLG NS RRI EL
Sbjct: 61  SPPDCYLNPVPQQSFQFDSALSSMVSSPAPSNSNPTNDSFVIRELIGKLGANSGRRISEL 120

Query: 121 NSTAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEKL 180
           NS++    SSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGN+RS+LSI NEKL
Sbjct: 121 NSSS----SSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNFRSNLSIANEKL 180

Query: 181 SRVSSSPSLKALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPVV 240
           SRVSSSPSLKALGSET LQE KNNSSSQEDESSLSNPDK ISNPRKRKAI+K KLKEP  
Sbjct: 181 SRVSSSPSLKALGSETKLQEQKNNSSSQEDESSLSNPDKAISNPRKRKAISKAKLKEPNA 240

Query: 241 EAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKDYIH 300
           E  PEKGSPKK+KAVETKE    NVKTEED KKTD +LA EEKQ K N+ PPE PKDYIH
Sbjct: 241 E-TPEKGSPKKIKAVETKE----NVKTEEDSKKTDADLA-EEKQQKTNTNPPEPPKDYIH 300

Query: 301 VRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQHQ 360
           VRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVTGKALMLDEIINYVQSLQHQ
Sbjct: 301 VRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQHQ 360

Query: 361 VEFLSMKLASVNTTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSSLPT 420
           VEFLSMKLASVNTTRVDFNMDSLI KQTYQS TSL H    PIDSSASS+YGH +   P+
Sbjct: 361 VEFLSMKLASVNTTRVDFNMDSLILKQTYQSATSLPH----PIDSSASSYYGHHHHQKPS 420

Query: 421 TSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYPNFG-EDELQSIVQMGFVQNQTQ 476
               S+DPLDSV CQNLPIQLPPL+SF  NPSQYPNFG EDEL SIV MGFVQNQ Q
Sbjct: 421 LPATSMDPLDSVSCQNLPIQLPPLSSFPLNPSQYPNFGEEDELLSIVHMGFVQNQAQ 462

BLAST of Lsi02G021330 vs. NCBI nr
Match: XP_038900265.1 (transcription factor bHLH62 isoform X1 [Benincasa hispida])

HSP 1 Score: 854.7 bits (2207), Expect = 3.8e-244
Identity = 467/497 (93.96%), Postives = 473/497 (95.17%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPFSSAMDVPPHSQSQLQVPELNHC-SSSHQ 60
           M+NEFFLN TGISQSLMHFDSNPSMATWHH FSSAMDVPP      QVPELNHC SSSHQ
Sbjct: 1   MDNEFFLNPTGISQSLMHFDSNPSMATWHHQFSSAMDVPP------QVPELNHCSSSSHQ 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL
Sbjct: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120

Query: 121 N-STAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEK 180
           N ST ALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRS LSI NEK
Sbjct: 121 NSSTPALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSRLSIGNEK 180

Query: 181 LSRVSSSPSLKALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPV 240
           LSRVSSSPSLKALGSETNLQEHK NSSSQEDESSLSNPDKTISNPRKRKAITKPKLKE V
Sbjct: 181 LSRVSSSPSLKALGSETNLQEHKKNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKESV 240

Query: 241 VEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKDYI 300
           VEA PEKGSPKKLKAVE+KE    NVKTEEDLKK+DENL  EEKQTKANSKPPEAPKDYI
Sbjct: 241 VEATPEKGSPKKLKAVESKE----NVKTEEDLKKSDENLG-EEKQTKANSKPPEAPKDYI 300

Query: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQH 360
           HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVTGKALMLDEIINYVQSLQH
Sbjct: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQH 360

Query: 361 QVEFLSMKLASVNTTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSSLP 420
           QVEFLSMKLASVNTTRVDFNMDSLISKQ YQSGTSLTHPQISPIDSSASSFYGHQNSSLP
Sbjct: 361 QVEFLSMKLASVNTTRVDFNMDSLISKQMYQSGTSLTHPQISPIDSSASSFYGHQNSSLP 420

Query: 421 TTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQEIS 480
           TTSHCSVDP+DSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQ+IVQMGF+QNQTQEIS
Sbjct: 421 TTSHCSVDPIDSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQNIVQMGFLQNQTQEIS 480

Query: 481 LQSHNFNLGSDQMKIEL 496
           LQSHNFNLGSDQMKIEL
Sbjct: 481 LQSHNFNLGSDQMKIEL 486

BLAST of Lsi02G021330 vs. NCBI nr
Match: XP_004150900.2 (transcription factor bHLH62 [Cucumis sativus] >KGN44647.1 hypothetical protein Csa_015829 [Cucumis sativus])

HSP 1 Score: 813.5 bits (2100), Expect = 9.8e-232
Identity = 451/500 (90.20%), Postives = 462/500 (92.40%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPFSSAMDVPPHSQSQLQVPELNHC-SSSHQ 60
           M+N+FFLN TGISQSLMHFDSNPS+ATWH  FSS MD+P       Q+P+L HC SSSHQ
Sbjct: 1   MDNQFFLNPTGISQSLMHFDSNPSIATWHQQFSSPMDLP------TQLPDLTHCSSSSHQ 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDCFLNPV DQSFQFDSALSSMVSSPAASNSN+TNESF IRELIGKLGGNSERRILEL
Sbjct: 61  SPPDCFLNPVSDQSFQFDSALSSMVSSPAASNSNITNESFAIRELIGKLGGNSERRILEL 120

Query: 121 -NST--AALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITN 180
            NST  AAL PSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSI N
Sbjct: 121 SNSTAAAALPPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSIGN 180

Query: 181 EKLSRVSSSPSLKALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKE 240
           EKLSRVSSSPSLKALGSE NLQEHKNNSSSQEDESSLSN DKTI+NPRKRKAITK KLKE
Sbjct: 181 EKLSRVSSSPSLKALGSEMNLQEHKNNSSSQEDESSLSNQDKTITNPRKRKAITKAKLKE 240

Query: 241 PVVEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKD 300
           PVVEA PEK SPKKLK VE KE    NVKTEEDLKK DEN A EE+QTKANSKPPEAPKD
Sbjct: 241 PVVEATPEKESPKKLKTVERKE----NVKTEEDLKKNDENSA-EERQTKANSKPPEAPKD 300

Query: 301 YIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSL 360
           YIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVTGKALMLDEIINYVQSL
Sbjct: 301 YIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSL 360

Query: 361 QHQVEFLSMKLASVNTTRVDFNMDSLI-SKQTYQSGTSLTHPQISPIDSSASSFYGHQNS 420
           QHQVEFLSMKLASVNTTRVDFN+DSLI SKQ YQSGTSLTHPQISPIDSS SSFYGHQNS
Sbjct: 361 QHQVEFLSMKLASVNTTRVDFNVDSLISSKQMYQSGTSLTHPQISPIDSSTSSFYGHQNS 420

Query: 421 SLPTTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQ 480
           SLPTTSHCSVDP+DSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQ
Sbjct: 421 SLPTTSHCSVDPIDSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQ 480

Query: 481 EISLQSHNFNLGSDQMKIEL 496
           EISLQSHNFNLGSDQMKIEL
Sbjct: 481 EISLQSHNFNLGSDQMKIEL 489

BLAST of Lsi02G021330 vs. NCBI nr
Match: XP_038900266.1 (transcription factor bHLH78 isoform X2 [Benincasa hispida])

HSP 1 Score: 796.6 bits (2056), Expect = 1.2e-226
Identity = 442/497 (88.93%), Postives = 448/497 (90.14%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPFSSAMDVPPHSQSQLQVPELNHC-SSSHQ 60
           M+NEFFLN TGISQSLMHFDSNPSMATWHH FSSAMDVPP      QVPELNHC SSSHQ
Sbjct: 1   MDNEFFLNPTGISQSLMHFDSNPSMATWHHQFSSAMDVPP------QVPELNHCSSSSHQ 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL
Sbjct: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120

Query: 121 N-STAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEK 180
           N ST ALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRS LSI NEK
Sbjct: 121 NSSTPALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSRLSIGNEK 180

Query: 181 LSRVSSSPSLKALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPV 240
           LSRVSSSPSLKALGSETNLQEHK NSSSQEDESSLSNPDKTISNPRKRKAITKPKLKE V
Sbjct: 181 LSRVSSSPSLKALGSETNLQEHKKNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKESV 240

Query: 241 VEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKDYI 300
           VEA PEKGSPKKLKAVE+KE    NVKTEEDLKK+DENL  EEKQTKANSKPPEAPKDYI
Sbjct: 241 VEATPEKGSPKKLKAVESKE----NVKTEEDLKKSDENLG-EEKQTKANSKPPEAPKDYI 300

Query: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQH 360
           HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVTGKALMLDEIINYVQSLQH
Sbjct: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQH 360

Query: 361 QVEFLSMKLASVNTTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSSLP 420
           QVE                          YQSGTSLTHPQISPIDSSASSFYGHQNSSLP
Sbjct: 361 QVEM-------------------------YQSGTSLTHPQISPIDSSASSFYGHQNSSLP 420

Query: 421 TTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQEIS 480
           TTSHCSVDP+DSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQ+IVQMGF+QNQTQEIS
Sbjct: 421 TTSHCSVDPIDSVLCQNLPIQLPPLNSFLQNPSQYPNFGEDELQNIVQMGFLQNQTQEIS 461

Query: 481 LQSHNFNLGSDQMKIEL 496
           LQSHNFNLGSDQMKIEL
Sbjct: 481 LQSHNFNLGSDQMKIEL 461

BLAST of Lsi02G021330 vs. NCBI nr
Match: KAG7014528.1 (Transcription factor bHLH62 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 745.7 bits (1924), Expect = 2.5e-211
Identity = 423/502 (84.26%), Postives = 445/502 (88.65%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPF-SSAMDVPPHSQSQLQVPELNHCSSSHQ 60
           MEN+FFLN+TGI QSLMHFDSNPSMATWHH F SSAMDVPP    Q  +P+L HCSSSHQ
Sbjct: 1   MENQFFLNATGIPQSLMHFDSNPSMATWHHQFSSSAMDVPP----QPHLPDLTHCSSSHQ 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIR+LIGKLGGNSE RILEL
Sbjct: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRQLIGKLGGNSEGRILEL 120

Query: 121 NSTAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEKL 180
           NSTAALTP SSSVAEF+SDPG AERAARFSCFGSRSFN RQLTNEFGNYRSHL I  EKL
Sbjct: 121 NSTAALTP-SSSVAEFTSDPGLAERAARFSCFGSRSFNDRQLTNEFGNYRSHLPIATEKL 180

Query: 181 SRVSSSPSLKALGSETN-LQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPV 240
           SRVSSSPS+KALGSETN L++HKNNSSS EDESSLSNPDK I NPRKRKAITK KLKEP+
Sbjct: 181 SRVSSSPSIKALGSETNLLEQHKNNSSSHEDESSLSNPDKAIPNPRKRKAITKAKLKEPI 240

Query: 241 VEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKDYI 300
           VE APEK  PKKLKAVE KENEKGN+KTEED +K DE  A EEKQTK NSKPPE PKDYI
Sbjct: 241 VE-APEKEIPKKLKAVEPKENEKGNMKTEEDSQKIDEKTA-EEKQTKENSKPPEPPKDYI 300

Query: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQH 360
           HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVTGKALMLDEIINYVQSLQH
Sbjct: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQH 360

Query: 361 QVEFLSMKLASVN--TTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSS 420
           QVEFLSMKLASVN  TTRVDFN+DSLISKQ Y+SGTSL HPQISPI+SS S FYGH N S
Sbjct: 361 QVEFLSMKLASVNTTTTRVDFNVDSLISKQMYESGTSLRHPQISPIESSGSRFYGHTNPS 420

Query: 421 LPTTSHCSVDPLDSVLCQNLPIQLPPL--NSFLQN-PSQYPNFGEDELQSIVQMGFVQNQ 480
           LPTTSHCSVDPLDSVL QNLPIQ PP   +SF QN PSQY +FGEDELQS+VQMGFVQNQ
Sbjct: 421 LPTTSHCSVDPLDSVLRQNLPIQHPPSLNSSFPQNHPSQYRSFGEDELQSMVQMGFVQNQ 480

Query: 481 TQEISLQSHNFNLGSDQMKIEL 496
           TQ  ++ +H    GS+QMKIEL
Sbjct: 481 TQNFNIGNH---AGSEQMKIEL 492

BLAST of Lsi02G021330 vs. NCBI nr
Match: KAG6576007.1 (Transcription factor basic helix-loop-helix 62, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 741.5 bits (1913), Expect = 4.7e-210
Identity = 423/502 (84.26%), Postives = 445/502 (88.65%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPF-SSAMDVPPHSQSQLQVPELNHCSSSHQ 60
           MEN+FFLN+TGI QSLMHFDSNPSMATWHH F SSAMDVPP    Q  +P+L HCSSSHQ
Sbjct: 1   MENQFFLNATGIPQSLMHFDSNPSMATWHHQFSSSAMDVPP----QPHLPDLTHCSSSHQ 60

Query: 61  SPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRELIGKLGGNSERRILEL 120
           SPPDCFLN VPDQSFQFDSALSSMVSSPAASNSNLTNESFVIR+LIGKLGGNSE RILEL
Sbjct: 61  SPPDCFLNSVPDQSFQFDSALSSMVSSPAASNSNLTNESFVIRQLIGKLGGNSEGRILEL 120

Query: 121 NSTAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEKL 180
           NSTAALTP SSSVAEF+SDPG AERAARFSCFGSRSFN RQLTNEFGNYRSHL I  EKL
Sbjct: 121 NSTAALTP-SSSVAEFTSDPGLAERAARFSCFGSRSFNDRQLTNEFGNYRSHLPIATEKL 180

Query: 181 SRVSSSPSLKALGSETN-LQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPV 240
           SRVSSSPS+KALGSETN L++HKNNSSS EDESSLSNPDK I NPRKRKAITK KLKEP+
Sbjct: 181 SRVSSSPSIKALGSETNLLEQHKNNSSSHEDESSLSNPDKAIPNPRKRKAITKAKLKEPI 240

Query: 241 VEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEEEKQTKANSKPPEAPKDYI 300
           VE APEK  PKKLKAVE KENEKGN+KTEED +K DE  A EEKQTK NSKPPE PKDYI
Sbjct: 241 VE-APEKEIPKKLKAVEPKENEKGNMKTEEDSQKIDEKTA-EEKQTKENSKPPEPPKDYI 300

Query: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQH 360
           HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVTGKALMLDEIINYVQSLQH
Sbjct: 301 HVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQH 360

Query: 361 QVEFLSMKLASVN--TTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSS 420
           QVEFLSMKLASVN  TTRVDFN+DSLISKQ Y+SGTS+ HPQISPI+SS S FYGH N S
Sbjct: 361 QVEFLSMKLASVNTTTTRVDFNVDSLISKQMYESGTSVRHPQISPIESSGSRFYGHTNPS 420

Query: 421 LPTTSHCSVDPLDSVLCQNLPIQ-LPPLN-SFLQN-PSQYPNFGEDELQSIVQMGFVQNQ 480
           LPTTSHCSVDPLDSVL QNLPIQ  PPLN SF QN PSQY +FGEDELQS+VQMGFVQNQ
Sbjct: 421 LPTTSHCSVDPLDSVLRQNLPIQHPPPLNSSFPQNHPSQYRSFGEDELQSMVQMGFVQNQ 480

Query: 481 TQEISLQSHNFNLGSDQMKIEL 496
           TQ  ++ +H    GS+QMKIEL
Sbjct: 481 TQNFNIGNH---AGSEQMKIEL 492

BLAST of Lsi02G021330 vs. TAIR 10
Match: AT5G48560.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 281.2 bits (718), Expect = 1.6e-75
Identity = 243/558 (43.55%), Postives = 301/558 (53.94%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMAT-WHHPFSSA---------MDVPPHSQSQLQVPE 60
           M+NE F+N+         F   P MAT + H  SS+         MD  PH  S      
Sbjct: 1   MDNELFMNT--------EFPPPPEMATHFEHQQSSSSAMMLNWALMDPNPHQDSSF---- 60

Query: 61  LNHCSSSHQSPPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLT---NESFVIRELIGK 120
           L   S+  Q            Q   FDSALSS+VSSP  SNSN +    + F+IRELIGK
Sbjct: 61  LWEKSTEQQ-----------QQQSIFDSALSSLVSSPTPSNSNFSGGGGDGFLIRELIGK 120

Query: 121 LG--GNSERRILEL-------NSTAALTPSSS-----------------SVAEFSSDPGF 180
           LG  GN+     E+       +++   TP SS                  + EFS+DPGF
Sbjct: 121 LGNIGNNNNNSGEIYGTPMSRSASCYATPMSSPPPPTNSNSQMMMNRTTPLTEFSADPGF 180

Query: 181 AERAARFSCFGSRSFNGRQLTNEFGNYRSHLSITNEKLSRVSSSPSLKALGSETNLQEHK 240
           AERAARFSCFGSRSFNGR  TN   N  +++   + KL+RVSS+P+LKAL S        
Sbjct: 181 AERAARFSCFGSRSFNGRTNTNLPINNGNNMVNNSGKLTRVSSTPALKALVS-------- 240

Query: 241 NNSSSQEDESSLSNPDKTISN--PRKRKAITKPKLKE-PVVEAAPEKGSPKKLKAVETKE 300
                         P+ T      RKRK++ K K KE P+  A+P   SP   K  E K 
Sbjct: 241 --------------PEVTPGGEFSRKRKSVPKGKSKENPISTASP---SPSFSKTAE-KN 300

Query: 301 NEKGNVKTEED----LKKTDENLAEEEKQTKA----NSKPPEAPKDYIHVRARRGQATDS 360
             KG  K+ E+     ++ +E+  EEE + +     N+KPPE PKDYIHVRARRGQATDS
Sbjct: 301 GGKGGSKSSEEKGGKRRREEEDDEEEEGEGEGNKSNNTKPPEPPKDYIHVRARRGQATDS 360

Query: 361 HSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQHQVEFLSMKLASV 420
           HSLAERVRREKI ERMKLLQDLVPG NKVTGKALMLDEIINYVQSLQ QVEFLSMKL+SV
Sbjct: 361 HSLAERVRREKIGERMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQRQVEFLSMKLSSV 420

Query: 421 NTTRVDFNMDSLISKQT-YQSGTSLTHPQISPIDSSAS------SFYGHQNSSLPTTSHC 480
           N TR+DFN+D+L+SK     S  +  H +     SS+       + Y + +  LP  S  
Sbjct: 421 NDTRLDFNVDALVSKDVMIPSSNNRLHEEGLQSKSSSHHHQQQLNIYNNNSQLLPNISSN 480

Query: 481 SV---DPLDSVLCQNLP---IQLPPLNSFLQNPSQYPNFGEDELQSIVQMGFVQNQTQEI 496
           ++    P++S+    L      LP L  F  + SQY  F E++LQSIV MG  +N   E 
Sbjct: 481 NMMLQSPMNSLETSTLARSFTHLPTLTQFTDSISQYQMFSEEDLQSIVGMGVAENPNNE- 498

BLAST of Lsi02G021330 vs. TAIR 10
Match: AT3G07340.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.7e-72
Identity = 224/509 (44.01%), Postives = 274/509 (53.83%), Query Frame = 0

Query: 1   MENEFFLNSTGISQSLMHFDSNPSMATWHHPFSSAMDVPPHSQSQLQVPELNHCSSSHQS 60
           MENE F+N+ G+S                HP    M  P  S + L+   +         
Sbjct: 1   MENELFMNA-GVS----------------HP--PVMTSPSSSSAMLKWVSMETQPVDPSL 60

Query: 61  PPDCFLNPVPDQSFQFDSALSSMVSSPAASNSNLT-----NESFVIRELIGKLG--GNSE 120
             + F     +QS  FDSALSS+VSSP  SNSN +      E+ ++RELIGKLG  G+  
Sbjct: 61  SRNLFWEKSTEQSI-FDSALSSLVSSPTPSNSNFSVGGVGGENVIMRELIGKLGNIGDIY 120

Query: 121 RRILELNSTAALTPSSS-------------SVAEFSSDPGFAERAARFSCFGSRSFNGRQ 180
                  ++   TP SS              +AE S DPGFAERAARFSCFGSRSFN R 
Sbjct: 121 GITASNGNSCYATPMSSPPPGSMMETKTTTPMAELSGDPGFAERAARFSCFGSRSFNSR- 180

Query: 181 LTNEFGNYRSHLSITNEKLSRVSSSPSLKALGSETNLQEHKNNSSSQEDESSLSNPDKTI 240
             + F         TNEK+ RVSSSP  K L S            + E    LS   KT 
Sbjct: 181 TNSPFPINNEPPITTNEKMPRVSSSPVFKPLASHV---------PAGESSGELSRKRKTK 240

Query: 241 SNPRKRKAITKPKLKEPVVEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLAEE 300
           S      A++  K  E       E   PK+ K            K+EE+  KT       
Sbjct: 241 SKQNSPSAVSSSKEIE-----EKEDSDPKRCK------------KSEENGDKT------- 300

Query: 301 EKQTKANSKPPEAPKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVT 360
                   K  +  KDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPG NKVT
Sbjct: 301 --------KSIDPYKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGCNKVT 360

Query: 361 GKALMLDEIINYVQSLQHQVEFLSMKLASVNTTRVDFNMDSLISKQTYQSGTSLT-HPQI 420
           GKALMLDEIINYVQSLQ QVEFLSMKL+SVN TR+DFNMD+L+SK  + S  +L  H Q+
Sbjct: 361 GKALMLDEIINYVQSLQRQVEFLSMKLSSVN-TRLDFNMDALLSKDIFPSSNNLMHHQQV 420

Query: 421 SPIDSSASSFYG-HQNSSL----PTTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYP 480
             +DSSA +  G H N +L      +S+  ++PL++   ++    LP L  F  + SQY 
Sbjct: 421 LQLDSSAETLLGDHHNKNLQLNPDISSNNVINPLETSETRSFISHLPTLAHFTDSISQYS 446

Query: 481 NFGEDELQSIVQMGFVQNQTQEISLQSHN 484
            F ED+L SI+ MGF QN+ QE++  S N
Sbjct: 481 TFSEDDLHSIIHMGFAQNRLQELNQGSSN 446

BLAST of Lsi02G021330 vs. TAIR 10
Match: AT3G23690.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 170.2 bits (430), Expect = 4.0e-42
Identity = 142/319 (44.51%), Postives = 186/319 (58.31%), Query Frame = 0

Query: 189 KALGSETNLQEHKNNSSSQEDESSLSNPDKTISNPRKRKAITKPKLKEPVVEAAPEKGSP 248
           +  GS+   QE  N SS  + +S       T SN RKRK+I     KE     +P   S 
Sbjct: 81  REFGSQPTTQE-SNKSSLLDPDSVSDRVHTTKSNSRKRKSIPSGNGKE-----SPASSSL 140

Query: 249 KKLKAVETKEN--EKGNVKTEEDLKKTDENLAEE---EKQTKANSKPPEAPKDYIHVRAR 308
               +  + EN   KG  ++++D+  + +N  E+   +   K ++KPPEAPKDYIHVRAR
Sbjct: 141 TASNSKVSGENGGSKGGKRSKQDVAGSSKNGVEKCDSKGDNKDDAKPPEAPKDYIHVRAR 200

Query: 309 RGQATDSHSLAERVRREKISERMKLLQDLVPGRNKVTGKALMLDEIINYVQSLQHQVEFL 368
           RGQATDSHSLAER RREKISERM LLQDLVPG N++TGKA+MLDEIINYVQSLQ QVEFL
Sbjct: 201 RGQATDSHSLAERARREKISERMTLLQDLVPGCNRITGKAVMLDEIINYVQSLQRQVEFL 260

Query: 369 SMKLASVNTTRVDFNMDSLISKQTYQSGTSLTHPQISPIDSSASSFYGHQNSSLPTTSHC 428
           SMKLA+VN  R++FN ++ +S +  Q G SLT            S Y            C
Sbjct: 261 SMKLATVN-PRMEFNANASLSTEMIQPGESLT-----------QSLY---------AMAC 320

Query: 429 SVDPLDSV---LCQNLP----IQLPPLNSFLQNPSQYPNFGE-DELQSIVQMGFVQNQTQ 488
           S   L S    L +N+P     Q P  + F+   ++ P F E ++LQSIVQMGF     Q
Sbjct: 321 SEQRLPSAYYSLGKNMPRFSDTQFPSNDGFVH--TETPGFWENNDLQSIVQMGFGDILQQ 370

Query: 489 EISLQSHNFNLGSDQMKIE 495
           + +  ++N +  + QMK+E
Sbjct: 381 QSNNNNNNCSEPTLQMKLE 370

BLAST of Lsi02G021330 vs. TAIR 10
Match: AT1G68920.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.6e-35
Identity = 146/398 (36.68%), Postives = 208/398 (52.26%), Query Frame = 0

Query: 120 NSTAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFN---GRQLTNE------------ 179
           N+++ L P   S+A+F +D GF ERAARFS F   +F+    + L N             
Sbjct: 114 NASSFLPP---SMAQFPADSGFIERAARFSLFSGGNFSDMVNQPLGNSEAIGLFLQGGGT 173

Query: 180 -FGNYRSHLSITNEKLSRVSSSPSLKALGSETNLQEHKNNSS--SQEDESSLSNPDK--- 239
             G  +S+     E  + VS +     + S    + +   S   S++ +SS  N  K   
Sbjct: 174 MQGQCQSNELNVGEPHNDVSVAVKESTVRSSEQAKPNVPGSGNVSEDTQSSGGNGQKGRE 233

Query: 240 TISNPRKRKAITKPKLKEPVVEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLA 299
           T SN +KRK     +  +   EAA    S +  +  +   +EK N   E+      +   
Sbjct: 234 TSSNTKKRK-----RNGQKNSEAAQSHRSQQSEEEPDNNGDEKRN--DEQSPNSPGKKSN 293

Query: 300 EEEKQTKANSKPPEAPKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNK 359
             ++Q K +S PP+    YIHVRARRGQAT+SHSLAERVRREKISERMK LQDLVPG NK
Sbjct: 294 SGKQQGKQSSDPPK--DGYIHVRARRGQATNSHSLAERVRREKISERMKFLQDLVPGCNK 353

Query: 360 VTGKALMLDEIINYVQSLQHQVEFLSMKLASVNTTRVDFNMDSLISKQTYQ--SGTSLTH 419
           VTGKA+MLDEIINYVQSLQ QVEFLSMKLA+VN  ++DFN++ L++K   Q  +G+S T 
Sbjct: 354 VTGKAVMLDEIINYVQSLQRQVEFLSMKLATVN-PQMDFNLEGLLAKDALQLRAGSSSTT 413

Query: 420 PQISPIDSSASSFYGHQNSSLPTTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYPNF 479
           P           F  + + + P   H  +    S + + +   L P+N   +   Q  N 
Sbjct: 414 P-----------FPPNMSMAYPPLPHGFMQQTLSSIGRTITSPLSPMNGGFKR--QETNG 473

Query: 480 GEDELQSIVQMGFVQNQTQEISLQSHNFNLGSDQMKIE 495
            E +LQ+++ + +           +   +L +  MK+E
Sbjct: 474 WEGDLQNVIHINYGAGDVTPDPQAAATASLPAANMKVE 485

BLAST of Lsi02G021330 vs. TAIR 10
Match: AT1G68920.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.6e-35
Identity = 146/398 (36.68%), Postives = 208/398 (52.26%), Query Frame = 0

Query: 120 NSTAALTPSSSSVAEFSSDPGFAERAARFSCFGSRSFN---GRQLTNE------------ 179
           N+++ L P   S+A+F +D GF ERAARFS F   +F+    + L N             
Sbjct: 114 NASSFLPP---SMAQFPADSGFIERAARFSLFSGGNFSDMVNQPLGNSEAIGLFLQGGGT 173

Query: 180 -FGNYRSHLSITNEKLSRVSSSPSLKALGSETNLQEHKNNSS--SQEDESSLSNPDK--- 239
             G  +S+     E  + VS +     + S    + +   S   S++ +SS  N  K   
Sbjct: 174 MQGQCQSNELNVGEPHNDVSVAVKESTVRSSEQAKPNVPGSGNVSEDTQSSGGNGQKGRE 233

Query: 240 TISNPRKRKAITKPKLKEPVVEAAPEKGSPKKLKAVETKENEKGNVKTEEDLKKTDENLA 299
           T SN +KRK     +  +   EAA    S +  +  +   +EK N   E+      +   
Sbjct: 234 TSSNTKKRK-----RNGQKNSEAAQSHRSQQSEEEPDNNGDEKRN--DEQSPNSPGKKSN 293

Query: 300 EEEKQTKANSKPPEAPKDYIHVRARRGQATDSHSLAERVRREKISERMKLLQDLVPGRNK 359
             ++Q K +S PP+    YIHVRARRGQAT+SHSLAERVRREKISERMK LQDLVPG NK
Sbjct: 294 SGKQQGKQSSDPPK--DGYIHVRARRGQATNSHSLAERVRREKISERMKFLQDLVPGCNK 353

Query: 360 VTGKALMLDEIINYVQSLQHQVEFLSMKLASVNTTRVDFNMDSLISKQTYQ--SGTSLTH 419
           VTGKA+MLDEIINYVQSLQ QVEFLSMKLA+VN  ++DFN++ L++K   Q  +G+S T 
Sbjct: 354 VTGKAVMLDEIINYVQSLQRQVEFLSMKLATVN-PQMDFNLEGLLAKDALQLRAGSSSTT 413

Query: 420 PQISPIDSSASSFYGHQNSSLPTTSHCSVDPLDSVLCQNLPIQLPPLNSFLQNPSQYPNF 479
           P           F  + + + P   H  +    S + + +   L P+N   +   Q  N 
Sbjct: 414 P-----------FPPNMSMAYPPLPHGFMQQTLSSIGRTITSPLSPMNGGFKR--QETNG 473

Query: 480 GEDELQSIVQMGFVQNQTQEISLQSHNFNLGSDQMKIE 495
            E +LQ+++ + +           +   +L +  MK+E
Sbjct: 474 WEGDLQNVIHINYGAGDVTPDPQAAATASLPAANMKVE 485

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJL42.3e-7443.55Transcription factor bHLH78 OS=Arabidopsis thaliana OX=3702 GN=BHLH78 PE=1 SV=1[more]
Q9SRT22.4e-7144.01Transcription factor bHLH62 OS=Arabidopsis thaliana OX=3702 GN=BHLH62 PE=2 SV=1[more]
Q9LK485.7e-4144.51Transcription factor bHLH77 OS=Arabidopsis thaliana OX=3702 GN=BHLH77 PE=1 SV=1[more]
Q9CAA92.3e-3436.68Transcription factor bHLH49 OS=Arabidopsis thaliana OX=3702 GN=BHLH49 PE=1 SV=1[more]
Q9C6703.1e-3139.78Transcription factor bHLH76 OS=Arabidopsis thaliana OX=3702 GN=BHLH76 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K8F44.7e-23290.20BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G360030 PE=4 S... [more]
A0A6J1GRP78.7e-21084.26transcription factor bHLH62-like OS=Cucurbita moschata OX=3662 GN=LOC111456512 P... [more]
A0A6J1JX209.0e-20783.47transcription factor bHLH62-like OS=Cucurbita maxima OX=3661 GN=LOC111488239 PE=... [more]
A0A1S3C6Z01.6e-20382.04transcription factor bHLH78 OS=Cucumis melo OX=3656 GN=LOC103497607 PE=4 SV=1[more]
A0A6J1KVI51.9e-19683.23transcription factor bHLH62-like OS=Cucurbita maxima OX=3661 GN=LOC111497970 PE=... [more]
Match NameE-valueIdentityDescription
XP_038900265.13.8e-24493.96transcription factor bHLH62 isoform X1 [Benincasa hispida][more]
XP_004150900.29.8e-23290.20transcription factor bHLH62 [Cucumis sativus] >KGN44647.1 hypothetical protein C... [more]
XP_038900266.11.2e-22688.93transcription factor bHLH78 isoform X2 [Benincasa hispida][more]
KAG7014528.12.5e-21184.26Transcription factor bHLH62 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6576007.14.7e-21084.26Transcription factor basic helix-loop-helix 62, partial [Cucurbita argyrosperma ... [more]
Match NameE-valueIdentityDescription
AT5G48560.11.6e-7543.55basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G07340.11.7e-7244.01basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G23690.14.0e-4244.51basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G68920.11.6e-3536.68basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G68920.21.6e-3536.68basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 263..283
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 178..219
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..294
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 178..294
NoneNo IPR availablePANTHERPTHR12565:SF336BHLH TRANSCRIPTION FACTORcoord: 123..488
NoneNo IPR availablePANTHERPTHR12565:SF336BHLH TRANSCRIPTION FACTORcoord: 2..110
NoneNo IPR availableCDDcd18919bHLH_AtBPE_likecoord: 296..382
e-value: 9.08686E-59
score: 186.879
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 312..362
e-value: 7.9E-11
score: 52.0
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 310..357
e-value: 2.6E-6
score: 27.3
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 306..356
score: 14.919459
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 303..375
e-value: 2.3E-13
score: 52.0
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 304..371
IPR024097Basic helix-loop-helix leucine zipper transcription factorPANTHERPTHR12565STEROL REGULATORY ELEMENT-BINDING PROTEINcoord: 2..110
IPR024097Basic helix-loop-helix leucine zipper transcription factorPANTHERPTHR12565STEROL REGULATORY ELEMENT-BINDING PROTEINcoord: 123..488

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G021330.1Lsi02G021330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0046983 protein dimerization activity