Lsi02G021150 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G021150
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionOxidoreductase, zinc-binding dehydrogenase family protein
Locationchr02: 27333184 .. 27336891 (-)
RNA-Seq ExpressionLsi02G021150
SyntenyLsi02G021150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAAGCTCCTCATTCTCAAAGGAAAAACTATTGGTAAGCATTGGGCTATGGCGGCTTCCACTACTTCCAGCCTTATGAAAGCTGTTCTGTATAAAGCCTACGGCGGCGGATCGGCTGCTCTCAAGGTTGATTATTATTATTTATAATTTTTTTAATTAATATTATTTTTTCATTTTCATGTCTTGTTCTCTCTATTTAAACATTTCAAGTTGTTATAGGTTTTTACTTTATTATTAAGGGGCAATTGAGTTACTAAGTTTGAAGTTGTTTAGTGGAAGGAGTTAATTTATTTATATTTAGGGAGCAAAGTTATTTAATATAAGTTTAGAAACTCTGTGTTTTAGGTGCATCATTTAATTTAGAGTTCTGAAGTTAGTATGCTCGACCTTTTTTTTAAAAAAAATATATTTTTGAGGGATACTGGAGATATTAGAAACTTCTTGGAATATGAAGGATATGTTTGGTAGGAAATTTGATAACAGAAATTTGAAAACAATGGATTCAACAAGGACAAGATTGTATTTCATGTTTTCAAATGTGTTTGGTAGTAGATTCGAAAATTGAATTCTAATTTAAACAATTGTTTTAAGTGTGTTGGATACTATATTTATAAATCATAAAACTAGTTGTAGTTAAATACTAAATATTAGTTGACAATAAACTATTATTAATTAATTTATGAAAATGTTTTATGTTAAAAAAAATTTATATAATTATTATTTTATAATATTATATAATTTATAATGCATCACATAATACATATTTTAATTTTAAGAAAAATGAAATGAGTTTATAACATGACATATATATTTCTAATTTTTTTTATGGTTGTATAATATAATTCTACATTTTAAAATTTTAAAATTCAAAATCTAGATACAATGAAAACATAAAAATGTTGTTTTTATAATTTGCAATGTTTGGATCATAGAATCTGAAAACAGTTTTCGGTAATATAATTCAGGTTATCTACCAAACACATGTTTTATGAACTTAGTAAATTTGACAACATCAAATAGAATCTAAATTGTCAACCAAACAGACCCGAAGATCCCCAATAGGTAGACTTCAATATTGGACACTATCGAAGTTGGTGAGATGGAGTTATTCAATACTGTATGTGAAGTTAGTGGGCAGCACAAGGAGTTGATTATTTAACCAACTTCACCGAATTCATATTGGTTGCCAAACACTCCGAAGTGATTTGAAAGAACTTGAATAGAAACAATTCAATACATAAGGACGGTGAAACCTAGACTCTTAGAAGATTAGAGATCAAATGGAGAATAACACTCAAATATTAGAGACTAAAACATGTATTTAGCATTTTTTTTTTTTTGTTAATATTTTAAAAACTCAAAAACAGTCCAAATCACTTCAAAACGACCCTTTTTACTATCTCAATGTTGATGATTCAACCTTCCACTAGTAATATTGTTTATTTATGTTATGAATCTTATGATTTGTAGTTGAAATGTGAGTGTTTTATGTTGCTGCAGCATGCAGAAGTTCCCATTCCAAGACCTAAAAATGATGAAGTTTTGCTGAAAGTAGAAGCAGTTAGTCTGAATCCATTTGATTGGAAAATTCAAAATGGAGTCATACGTCCATTTTTTCCTCGCAACTTCCCTCATATTCCATGTGTGTCCTGAATTCTCTGCTCGACTTCAAAGCTTCTTATACTAAAGTTTGAGTTGGTGAGACCGTCACCATGTAACTGAAATTAGTTTTATCAATAGATGTACACGAGAAAAGTACCTCAAATTTATATCGATTATTAAAAGACCAAGCGTAAAACTTACCTCCTAATTGGTCACAAGGATAGCTAATTATTGTCTTGAAAAATGAAAAAAGTATTCTTCTTTCCTATTTGTAAAAATATAAAGGTCATAAGTAGGAAATTCATGTTGGCTAGAATAAGTTAAGTTTTGAATGAGGTGAGAGTTAGACCATTGTTAATACCTTTTTCTTATGAAAACCAATATATTGACAGACCAAAAATCTTGCTTATGAATTTGAAACAGGTACTGATGTGGCTGGAGAAGTCTTAGAGGTTGGAGCAGAGGTAAAAAGCTTAAAAGTTGGGGACAAAGTTGTGACATTTCTTAACCATGCTGTAAGATTTCACTCACCCTTTTCCACAGTTAATCTAGCTTTAACCACGATGAGCAACATCACAAAAGAAAATCATGACTTTGCTGATAAGTATCAAGTCTACATTTCTGTTCTGTATTTCAACTCTCTGTTACTGCACTTCTATATACAAATGTTTTAAACCCTCCATTTCATACCATTCTATTGTTAGATCACCGACTGGCCAAAAACTTTTGTGTCAATTGACGATTTAACATAGTATCAGAGCAGGATGTTTAAGAGGTCCTGTGTTCAAATCCTCGTAATAATGTCTCATTTCCTCTCCAAGTCATATTGATTTTACTTGTTGGGTATTAACCCAAAGACATACTCCCACACGTATTCATTGTCATTCTTCTTTTCTACACCTTTAAATTTGAGGACTTTCTTTTTCTTTCTGCCTTGAAATCTTCATTACCGCTCCTAAATGAGATTGAAGAGGTAGGAAGAAATGATTTTTTCTTGATGAGAGGGGTCAGATTTTTGTTGATAGACGGTTCGAGTTTCACGTGTCAAGTAGCACCCATATTTTGAATATGTTAATAAAACATAATTTAATGATACATCAACCTTTTGCTAACAAAATTTAACCATTGTTTTATGGAATAACCTAATTGAGTTGTTTTTATACGTTAGGGGTAAAATCCAGAATTTTAAAAGATTAAGGATTAAATTGAGAATTATGTGAAAAGCTTAAGGACTATCTGGTATATTTAGCCTTCAAAACATATCTTTCACAAGCTTAATATGTATGAATCCTCACCAATCTCTTATTAATCCTCATGGAGAAAGTTCTAATGCTCAAGAATTACATCATTGATTTATGTTATAGGTAAGTGTTCATCTTTACAATTCTTTTTGTATGGCCATGACAGACTGGAGGTGGACTTGCTGAATATGTTGTAGCAAAGGAAGCTTCAACAGTTTGTAGGCCGACAGAAGTGACCGCAGCCGATAGCGCTGCCCTGCCCGTTGCGGCTTTGACAGCACATGAAGCTCTCACCCAAGCTGCTGGGATCAAGCTTGATAGAAATGGCCCTCAATCAAACATTCTCATCACTGCTGCCTCTGGTGGTGTCGGGCATTACGCCGTTCAGCTAGCAAAGCTAGGAAACACACACGTGACCGCTACTTGCGGTGCCCGAAACGTCGAGTTTGTGAAGAGCTTGGGCGCAGATGAGGTCATAGATTACAGAACCTCAGAAGGGGCAACCTTGAAGAGTCCATCAGGTAAGAAATATGATGCAGTGATTCATTGTACAACAGGGATCCCTTGGTCTGTATTTGAGCCTAATTTGAGCTCAAACGGTAAGGTGATGGACATTACTCCGGCGAATGCCGCTACGGTGCTTACTTTTGCCTTTAAGAAACTTACATTTTCTAAGAAGAAGTTGGTGCCATTGTGGGGGATGTCTTTCAAGAGTGAGAATTTGAGGTATGTTGTGAATTTGGTGAAGGAAGGGAAGGTGAAGAGTGTGATTGATTCAAGGTATCCTTTGAGTAAGGCTGAGGAGGCTTGGGCTAAGAGCATGGATGGCCATGCCACTGGCAAAATCATTGTGGAGCCATAA

mRNA sequence

ATGTTTAAGCTCCTCATTCTCAAAGGAAAAACTATTGGTAAGCATTGGGCTATGGCGGCTTCCACTACTTCCAGCCTTATGAAAGCTGTTCTGTATAAAGCCTACGGCGGCGGATCGGCTGCTCTCAAGCATGCAGAAGTTCCCATTCCAAGACCTAAAAATGATGAAGTTTTGCTGAAAGTAGAAGCAGTTAGTCTGAATCCATTTGATTGGAAAATTCAAAATGGAGTCATACGTACTGATGTGGCTGGAGAAGTCTTAGAGGTTGGAGCAGAGGTAAAAAGCTTAAAAGTTGGGGACAAAGTTGTGACATTTCTTAACCATGCTACTGGAGGTGGACTTGCTGAATATGTTGTAGCAAAGGAAGCTTCAACAGTTTGTAGGCCGACAGAAGTGACCGCAGCCGATAGCGCTGCCCTGCCCGTTGCGGCTTTGACAGCACATGAAGCTCTCACCCAAGCTGCTGGGATCAAGCTTGATAGAAATGGCCCTCAATCAAACATTCTCATCACTGCTGCCTCTGGTGGTGTCGGGCATTACGCCGTTCAGCTAGCAAAGCTAGGAAACACACACGTGACCGCTACTTGCGGTGCCCGAAACGTCGAGTTTGTGAAGAGCTTGGGCGCAGATGAGGTCATAGATTACAGAACCTCAGAAGGGGCAACCTTGAAGAGTCCATCAGGTAAGAAATATGATGCAGTGATTCATTGTACAACAGGGATCCCTTGGTCTGTATTTGAGCCTAATTTGAGCTCAAACGGTAAGGTGATGGACATTACTCCGGCGAATGCCGCTACGGTGCTTACTTTTGCCTTTAAGAAACTTACATTTTCTAAGAAGAAGTTGGTGCCATTGTGGGGGATGTCTTTCAAGAGTGAGAATTTGAGGTATGTTGTGAATTTGGTGAAGGAAGGGAAGGTGAAGAGTGTGATTGATTCAAGGTATCCTTTGAGTAAGGCTGAGGAGGCTTGGGCTAAGAGCATGGATGGCCATGCCACTGGCAAAATCATTGTGGAGCCATAA

Coding sequence (CDS)

ATGTTTAAGCTCCTCATTCTCAAAGGAAAAACTATTGGTAAGCATTGGGCTATGGCGGCTTCCACTACTTCCAGCCTTATGAAAGCTGTTCTGTATAAAGCCTACGGCGGCGGATCGGCTGCTCTCAAGCATGCAGAAGTTCCCATTCCAAGACCTAAAAATGATGAAGTTTTGCTGAAAGTAGAAGCAGTTAGTCTGAATCCATTTGATTGGAAAATTCAAAATGGAGTCATACGTACTGATGTGGCTGGAGAAGTCTTAGAGGTTGGAGCAGAGGTAAAAAGCTTAAAAGTTGGGGACAAAGTTGTGACATTTCTTAACCATGCTACTGGAGGTGGACTTGCTGAATATGTTGTAGCAAAGGAAGCTTCAACAGTTTGTAGGCCGACAGAAGTGACCGCAGCCGATAGCGCTGCCCTGCCCGTTGCGGCTTTGACAGCACATGAAGCTCTCACCCAAGCTGCTGGGATCAAGCTTGATAGAAATGGCCCTCAATCAAACATTCTCATCACTGCTGCCTCTGGTGGTGTCGGGCATTACGCCGTTCAGCTAGCAAAGCTAGGAAACACACACGTGACCGCTACTTGCGGTGCCCGAAACGTCGAGTTTGTGAAGAGCTTGGGCGCAGATGAGGTCATAGATTACAGAACCTCAGAAGGGGCAACCTTGAAGAGTCCATCAGGTAAGAAATATGATGCAGTGATTCATTGTACAACAGGGATCCCTTGGTCTGTATTTGAGCCTAATTTGAGCTCAAACGGTAAGGTGATGGACATTACTCCGGCGAATGCCGCTACGGTGCTTACTTTTGCCTTTAAGAAACTTACATTTTCTAAGAAGAAGTTGGTGCCATTGTGGGGGATGTCTTTCAAGAGTGAGAATTTGAGGTATGTTGTGAATTTGGTGAAGGAAGGGAAGGTGAAGAGTGTGATTGATTCAAGGTATCCTTTGAGTAAGGCTGAGGAGGCTTGGGCTAAGAGCATGGATGGCCATGCCACTGGCAAAATCATTGTGGAGCCATAA

Protein sequence

MFKLLILKGKTIGKHWAMAASTTSSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIRTDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVCRPTEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKLGNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFEPNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSVIDSRYPLSKAEEAWAKSMDGHATGKIIVEP
Homology
BLAST of Lsi02G021150 vs. ExPASy Swiss-Prot
Match: Q9SV68 (Chloroplast envelope quinone oxidoreductase homolog OS=Arabidopsis thaliana OX=3702 GN=CEQORH PE=1 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 2.1e-119
Identity = 224/327 (68.50%), Postives = 265/327 (81.04%), Query Frame = 0

Query: 26  LMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR------ 85
           LM A+ Y +YGGG+A L+H +VP+P PK++EV LK+EA SLNP DWKIQ G+IR      
Sbjct: 5   LMHALQYNSYGGGAAGLEHVQVPVPTPKSNEVCLKLEATSLNPVDWKIQKGMIRPFLPRK 64

Query: 86  ------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVCRPTEVT 145
                 TDVAGEV+EVG+ VK+ K GDKVV  L+H  GGGLAE+ VA E  TV RP EV 
Sbjct: 65  FPCIPATDVAGEVVEVGSGVKNFKAGDKVVAVLSHLGGGGLAEFAVATEKLTVKRPQEVG 124

Query: 146 AADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKLGNTHVT 205
           AA++AALPVA LTA +ALT  AG+KLD  G ++NIL+TAASGGVGHYAVQLAKL N HVT
Sbjct: 125 AAEAAALPVAGLTALQALTNPAGLKLDGTGKKANILVTAASGGVGHYAVQLAKLANAHVT 184

Query: 206 ATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFEPNLSSN 265
           ATCGARN+EFVKSLGADEV+DY+T EGA LKSPSGKKYDAV+HC  GIP+SVFEPNLS N
Sbjct: 185 ATCGARNIEFVKSLGADEVLDYKTPEGAALKSPSGKKYDAVVHCANGIPFSVFEPNLSEN 244

Query: 266 GKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSVIDS 325
           GKV+DITP   A + T+A KK+T SKK+LVPL  +  K+ENL ++VNLVKEGKVK+VIDS
Sbjct: 245 GKVIDITPGPNA-MWTYAVKKITMSKKQLVPLL-LIPKAENLEFMVNLVKEGKVKTVIDS 304

Query: 326 RYPLSKAEEAWAKSMDGHATGKIIVEP 341
           ++PLSKAE+AWAKS+DGHATGKIIVEP
Sbjct: 305 KHPLSKAEDAWAKSIDGHATGKIIVEP 329

BLAST of Lsi02G021150 vs. ExPASy Swiss-Prot
Match: Q8H0M1 (Quinone-oxidoreductase homolog, chloroplastic OS=Spinacia oleracea OX=3562 GN=QOR PE=1 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 8.8e-118
Identity = 220/329 (66.87%), Postives = 260/329 (79.03%), Query Frame = 0

Query: 24  SSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR---- 83
           + LM A+ Y  YGGG+ ALKH EV +P PK+DE+LLK+EA +LNP DWKIQ GV+R    
Sbjct: 3   AKLMHAIQYSGYGGGTDALKHVEVAVPDPKSDELLLKIEAATLNPIDWKIQKGVLRPLLP 62

Query: 84  --------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVCRPTE 143
                   TDVAGEV++ G+ V   K GDKVV  L+HATGG LAEY VAKE  TV RP E
Sbjct: 63  RKFPTIPGTDVAGEVVQAGSAVNRFKTGDKVVAVLSHATGGALAEYAVAKENLTVARPPE 122

Query: 144 VTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKLGNTH 203
           V+AA+ AALPVAALTAH+ALTQ A IKLD +G + NILITAASGGVGHYAVQLAKLGNTH
Sbjct: 123 VSAAEGAALPVAALTAHQALTQFANIKLDGSGERKNILITAASGGVGHYAVQLAKLGNTH 182

Query: 204 VTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFEPNLS 263
           VTATCGARN++FVK LGADEV+DY+T EGA+L SPSGKKYD V+H  +GIPWS FEPNLS
Sbjct: 183 VTATCGARNLDFVKGLGADEVLDYKTPEGASLTSPSGKKYDYVVHGASGIPWSTFEPNLS 242

Query: 264 SNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSVI 323
             GKV+D+TP   A ++TFA+KKLTFSKK+LVPL  +  K  N  YVVNLVKE K+K+VI
Sbjct: 243 EAGKVIDLTPGPTA-MMTFAWKKLTFSKKQLVPLL-LIPKIPNFEYVVNLVKEKKLKTVI 302

Query: 324 DSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           DS++PLSK E+AW++ M GHATGKII+EP
Sbjct: 303 DSKHPLSKGEDAWSRIMGGHATGKIIIEP 329

BLAST of Lsi02G021150 vs. ExPASy Swiss-Prot
Match: Q9AYU1 (Quinone-oxidoreductase QR1, chloroplastic OS=Triphysaria versicolor OX=64093 PE=1 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 1.7e-113
Identity = 214/327 (65.44%), Postives = 254/327 (77.68%), Query Frame = 0

Query: 26  LMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR------ 85
           LM+AV Y  YGGG+A LKH EVPIP P   EVL+K+EA+SLN  DWK+QNG++R      
Sbjct: 5   LMRAVQYDGYGGGAAGLKHVEVPIPSPGKGEVLIKLEAISLNQLDWKLQNGMVRPFLPRK 64

Query: 86  ------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVCRPTEVT 145
                 TDVAGEV+ +G +VK+ K GDKVV  L    GGGLAEY VA E  TV RP EV+
Sbjct: 65  FPFIPATDVAGEVVRIGQDVKNFKPGDKVVAMLGSFGGGGLAEYGVASEKLTVHRPPEVS 124

Query: 146 AADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKLGNTHVT 205
           AA+S+ LP+A LTAH ALTQ  G+ LD++GP  NILITAASGGVG YAVQLAKLGNTHVT
Sbjct: 125 AAESSGLPIAGLTAHMALTQHIGLNLDKSGPHKNILITAASGGVGQYAVQLAKLGNTHVT 184

Query: 206 ATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFEPNLSSN 265
           ATCG+RN + VKSLGADEVIDY+T EGA LKSPSGKKYDAVIHC + +PWSVF+PNLS +
Sbjct: 185 ATCGSRNFDLVKSLGADEVIDYKTPEGAALKSPSGKKYDAVIHCASPLPWSVFKPNLSKH 244

Query: 266 GKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSVIDS 325
           GKV+DITP     +LT A  KLT SKK+LV L  +  K E+L Y+V L++EGK+K+VIDS
Sbjct: 245 GKVIDITP-GPRVMLTSAMTKLTCSKKRLVTLL-VVIKGEHLSYLVELMREGKLKTVIDS 304

Query: 326 RYPLSKAEEAWAKSMDGHATGKIIVEP 341
           ++ LSKAEEAWAKS+DGHATGKI+VEP
Sbjct: 305 KFSLSKAEEAWAKSIDGHATGKIVVEP 329

BLAST of Lsi02G021150 vs. ExPASy Swiss-Prot
Match: Q84V25 (2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.1e-30
Identity = 111/346 (32.08%), Postives = 159/346 (45.95%), Query Frame = 0

Query: 17  AMAASTTSSLMKAVLYKAYGGGSAALK-HAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQN 76
           A  + +  S+ KA +Y  YG  S  LK    V +P  K D+VL+KV A SLNP D+K   
Sbjct: 3   AAPSESIPSVNKAWVYSEYGKTSDVLKFDPSVAVPEVKEDQVLIKVVAASLNPVDFKRAL 62

Query: 77  GVIRT-----------DVAGEVLEVGAEVKSLKVGDKVVTFLNHAT------GGGLAEYV 136
           G  +            DVAG V++VG++V   KVGD+V   LN A        G LAEY 
Sbjct: 63  GYFKDTDSPLPTVPGYDVAGVVVKVGSQVTKFKVGDEVYGDLNEAALVNPTRFGSLAEYT 122

Query: 137 VAKEASTVCRPTEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVG 196
            A E     +P +++  ++A+LP+A  TA+E L +A            +IL+   +GGVG
Sbjct: 123 AADERVLAHKPKDLSFIEAASLPLAIETAYEGLERA------ELSAGKSILVLGGAGGVG 182

Query: 197 HYAVQLAK--LGNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIH 256
            + +QLAK   G + V AT   + ++F+++LG D  IDY       L       YDAV  
Sbjct: 183 THIIQLAKHVFGASKVAATASTKKLDFLRTLGVDLAIDYTKENIEDLPEKFDVVYDAVGE 242

Query: 257 CTTGIPWSVFEPNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLR 316
               +        +   GKV+ I        + F         +KL P            
Sbjct: 243 TDKAV------KAVKEGGKVVTIVGPATPPAIHFVLTSKGSVLEKLKP------------ 302

Query: 317 YVVNLVKEGKVKSVID--SRYPLSKAEEAWAKSMDGHATGKIIVEP 341
                ++ GKVK V+D  S YP +K  EA+       ATGK++V P
Sbjct: 303 ----YLESGKVKPVLDPTSPYPFTKLVEAFGYLESSRATGKVVVYP 320

BLAST of Lsi02G021150 vs. ExPASy Swiss-Prot
Match: Q9ZUC1 (NADPH-dependent alkenal/one oxidoreductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AOR PE=1 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 9.0e-30
Identity = 105/335 (31.34%), Postives = 150/335 (44.78%), Query Frame = 0

Query: 27  MKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIRT------ 86
           MKA +Y  YGG       + + +P  K D+VL+KV A +LNP D K + G  +       
Sbjct: 78  MKAWVYSDYGGVDVLKLESNIVVPEIKEDQVLIKVVAAALNPVDAKRRQGKFKATDSPLP 137

Query: 87  -----DVAGEVLEVGAEVKSLKVGDKVVTFLNHAT------GGGLAEYVVAKEASTVCRP 146
                DVAG V++VG+ VK LK GD+V   ++          G LAEY   +E     +P
Sbjct: 138 TVPGYDVAGVVVKVGSAVKDLKEGDEVYANVSEKALEGPKQFGSLAEYTAVEEKLLALKP 197

Query: 147 TEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAK--L 206
             +  A +A LP+A  TA E L +             +IL+   +GGVG   +QLAK   
Sbjct: 198 KNIDFAQAAGLPLAIETADEGLVRT------EFSAGKSILVLNGAGGVGSLVIQLAKHVY 257

Query: 207 GNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFE 266
           G + V AT     +E V+SLGAD  IDY       L       +DA+  C   +      
Sbjct: 258 GASKVAATASTEKLELVRSLGADLAIDYTKENIEDLPDKYDVVFDAIGMCDKAV------ 317

Query: 267 PNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKV 326
             +   GKV+ +T A       F         KKL P                 ++ GKV
Sbjct: 318 KVIKEGGKVVALTGAVTPPGFRFVVTSNGDVLKKLNP----------------YIESGKV 377

Query: 327 KSVIDSR--YPLSKAEEAWAKSMDGHATGKIIVEP 341
           K V+D +  +P S+  +A++     HATGK++V P
Sbjct: 378 KPVVDPKGPFPFSRVADAFSYLETNHATGKVVVYP 384

BLAST of Lsi02G021150 vs. ExPASy TrEMBL
Match: A0A6J1JX05 (chloroplast envelope quinone oxidoreductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111488227 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.9e-156
Identity = 288/333 (86.49%), Postives = 302/333 (90.69%), Query Frame = 0

Query: 20  ASTTSSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR 79
           AS TS LMKAVLY+AYGGGSAALKH EVPIP PK+DEVLLKVEAVSLNPFDWKIQ GV+R
Sbjct: 2   ASATSRLMKAVLYEAYGGGSAALKHVEVPIPGPKDDEVLLKVEAVSLNPFDWKIQKGVVR 61

Query: 80  ------------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVC 139
                       TDVAGEVLEVGA VK LK GDKVVTFLNHATGGGLAEYVVAKE+STV 
Sbjct: 62  LFLPRNFPHIPCTDVAGEVLEVGARVKGLKAGDKVVTFLNHATGGGLAEYVVAKESSTVV 121

Query: 140 RPTEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKL 199
           RP EVTAAD AALPVAALTAH+ALTQAAGIKLD NGPQSNILITAASGGVGHYAVQLAKL
Sbjct: 122 RPPEVTAADGAALPVAALTAHQALTQAAGIKLDANGPQSNILITAASGGVGHYAVQLAKL 181

Query: 200 GNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFE 259
           GNTHVTATCGARN+EFVKSLGADEVIDY+TSEGA LKSPSGKKYDAVIHC TGIPWSVFE
Sbjct: 182 GNTHVTATCGARNIEFVKSLGADEVIDYKTSEGAALKSPSGKKYDAVIHCATGIPWSVFE 241

Query: 260 PNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKV 319
           PNLSS GKV+DITPANA+TV+TFA KKLTFSKKKLVP+W MSFK+ENL YVVNL+KEGKV
Sbjct: 242 PNLSSTGKVVDITPANASTVMTFAIKKLTFSKKKLVPMWSMSFKNENLGYVVNLMKEGKV 301

Query: 320 KSVIDSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           KS IDSRYPLSKAEEAWAKSMDGHATGKIIV+P
Sbjct: 302 KSTIDSRYPLSKAEEAWAKSMDGHATGKIIVQP 334

BLAST of Lsi02G021150 vs. ExPASy TrEMBL
Match: A0A6J1GMN0 (chloroplast envelope quinone oxidoreductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111455901 PE=4 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 7.2e-155
Identity = 286/333 (85.89%), Postives = 301/333 (90.39%), Query Frame = 0

Query: 20  ASTTSSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR 79
           ASTTS LMKAV Y+AYGGGSAALKH EVPIP PK+DEVLLKVEAVSLNPFDWKIQ GV+R
Sbjct: 2   ASTTSRLMKAVRYEAYGGGSAALKHVEVPIPGPKDDEVLLKVEAVSLNPFDWKIQKGVVR 61

Query: 80  ------------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVC 139
                       TDVAGEVLEVG+ VK LKVGDKVVTFLNHATGGGLAEYVVAKEASTV 
Sbjct: 62  PFLPRNFPHIPCTDVAGEVLEVGSRVKRLKVGDKVVTFLNHATGGGLAEYVVAKEASTVI 121

Query: 140 RPTEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKL 199
           RP EVTAAD AALPVAALTAH+ALTQAAGIKLD NGPQ NILITAASGGVGHYAVQLAKL
Sbjct: 122 RPPEVTAADGAALPVAALTAHQALTQAAGIKLDANGPQLNILITAASGGVGHYAVQLAKL 181

Query: 200 GNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFE 259
           GNTHVTATCGARN+EFVKSLGADEVIDY+TSEGA LKSPSGKKYDAVIHC TGIPWSVFE
Sbjct: 182 GNTHVTATCGARNIEFVKSLGADEVIDYKTSEGAALKSPSGKKYDAVIHCATGIPWSVFE 241

Query: 260 PNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKV 319
           PNLSSNGKV+DITPAN +TV+TFA KKLTFSKKKLVP+W MSFK+ENL YV+NL KEGKV
Sbjct: 242 PNLSSNGKVVDITPANVSTVMTFAIKKLTFSKKKLVPMWSMSFKNENLEYVMNLTKEGKV 301

Query: 320 KSVIDSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           KS+IDSRY LSKAEEAWAKS+DGHATGKIIVEP
Sbjct: 302 KSMIDSRYALSKAEEAWAKSVDGHATGKIIVEP 334

BLAST of Lsi02G021150 vs. ExPASy TrEMBL
Match: A0A0A0K8E0 (PKS_ER domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G351910 PE=4 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.4e-142
Identity = 268/330 (81.21%), Postives = 292/330 (88.48%), Query Frame = 0

Query: 23  TSSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR--- 82
           TS LM+AVLY+AYGGGSAALKHAEVPIP+PK DEVL+KVEAVSLNP DWKIQ G+IR   
Sbjct: 2   TSRLMQAVLYEAYGGGSAALKHAEVPIPKPKTDEVLVKVEAVSLNPIDWKIQKGLIRPFF 61

Query: 83  ---------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVCRPT 142
                    TDVAGEV+EVGA VKSLK GDKVVT+L+HATGGGLAEY V KE+ TV RP 
Sbjct: 62  PRKFPHIPGTDVAGEVVEVGAGVKSLKAGDKVVTYLSHATGGGLAEYAVGKESVTVYRPP 121

Query: 143 EVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKLGNT 202
           EVTAAD AALPVAALTAH+ALTQ AG+KLD +GPQSNILITAASGGVGHYAVQLAKLGNT
Sbjct: 122 EVTAADGAALPVAALTAHQALTQVAGVKLDGSGPQSNILITAASGGVGHYAVQLAKLGNT 181

Query: 203 HVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFEPNL 262
           HVTATCGARN+EFVKSLGADEVIDYRT EGA LKSPSGKKYDAV+HC +GIPWSVFEPNL
Sbjct: 182 HVTATCGARNIEFVKSLGADEVIDYRTPEGAALKSPSGKKYDAVVHCASGIPWSVFEPNL 241

Query: 263 SSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSV 322
           S NGKV+DITP  AA + TFAFKKLTFSKK+LVPL  MSFKSENL Y++NL+KE K+K+V
Sbjct: 242 SPNGKVIDITPTPAA-LATFAFKKLTFSKKQLVPLL-MSFKSENLSYLINLIKERKLKTV 301

Query: 323 IDSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           IDSRYPLSKAE+AWAKSMDGHATGKIIVEP
Sbjct: 302 IDSRYPLSKAEDAWAKSMDGHATGKIIVEP 329

BLAST of Lsi02G021150 vs. ExPASy TrEMBL
Match: A0A1S3C6G6 (putative quinone-oxidoreductase homolog, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103497589 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 7.0e-142
Identity = 265/329 (80.55%), Postives = 291/329 (88.45%), Query Frame = 0

Query: 24  SSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR---- 83
           S LM+AVLY+AYGGGSA LKHAEVPIP+PK DEVL+KVEAVSLNP DWKIQ G+IR    
Sbjct: 3   SRLMQAVLYEAYGGGSAGLKHAEVPIPKPKTDEVLVKVEAVSLNPIDWKIQKGLIRPFFP 62

Query: 84  --------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVCRPTE 143
                   TDVAGEV+EVGA VKSLK GDKVVT+L+HATGGGLAEY V KE+ TV RP E
Sbjct: 63  RKFPHIPGTDVAGEVVEVGAGVKSLKAGDKVVTYLSHATGGGLAEYAVGKESVTVYRPPE 122

Query: 144 VTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKLGNTH 203
           VTAAD AALPVAALTAH+ALTQ AG+KLD +GPQSNILITAASGGVGHYAVQ+AKLGNTH
Sbjct: 123 VTAADGAALPVAALTAHQALTQVAGVKLDGSGPQSNILITAASGGVGHYAVQIAKLGNTH 182

Query: 204 VTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFEPNLS 263
           VTATCGARN+EFVKSLGADEVIDY+T EGA LKSPSGKKYDAV+HC +GIPWSVFEPNLS
Sbjct: 183 VTATCGARNIEFVKSLGADEVIDYKTPEGAALKSPSGKKYDAVVHCASGIPWSVFEPNLS 242

Query: 264 SNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSVI 323
            NGKV+DITP  AA + TFAFKKLTFSKK+LVPL  MSFKSENL Y++NL+KEGK+K+VI
Sbjct: 243 PNGKVIDITPTPAA-LATFAFKKLTFSKKQLVPLL-MSFKSENLSYLINLIKEGKLKTVI 302

Query: 324 DSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           DSRYPLSKAE+AWAKSMDGHATGKIIVEP
Sbjct: 303 DSRYPLSKAEDAWAKSMDGHATGKIIVEP 329

BLAST of Lsi02G021150 vs. ExPASy TrEMBL
Match: A0A5A7SP07 (Putative quinone-oxidoreductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold263G00610 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.2e-141
Identity = 265/329 (80.55%), Postives = 290/329 (88.15%), Query Frame = 0

Query: 24  SSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR---- 83
           S LM AVLY+AYGGGSA LKHAEVPIP+PK DEVL+KVEAVSLNP DWKIQ G+IR    
Sbjct: 3   SRLMHAVLYEAYGGGSAGLKHAEVPIPKPKTDEVLVKVEAVSLNPIDWKIQKGLIRPFFP 62

Query: 84  --------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVCRPTE 143
                   TDVAGEV+EVGA VKSLK GDKVVT+L+HATGGGLAEY V KE+ TV RP E
Sbjct: 63  RKFPHIPGTDVAGEVVEVGAGVKSLKAGDKVVTYLSHATGGGLAEYAVGKESVTVYRPPE 122

Query: 144 VTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKLGNTH 203
           VTAAD AALPVAALTAH+ALTQ AG+KLD +GPQSNILITAASGGVGHYAVQ+AKLGNTH
Sbjct: 123 VTAADGAALPVAALTAHQALTQVAGVKLDGSGPQSNILITAASGGVGHYAVQIAKLGNTH 182

Query: 204 VTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFEPNLS 263
           VTATCGARN+EFVKSLGADEVIDY+T EGA LKSPSGKKYDAV+HC +GIPWSVFEPNLS
Sbjct: 183 VTATCGARNIEFVKSLGADEVIDYKTPEGAALKSPSGKKYDAVVHCASGIPWSVFEPNLS 242

Query: 264 SNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSVI 323
            NGKV+DITP  AA + TFAFKKLTFSKK+LVPL  MSFKSENL Y++NL+KEGK+K+VI
Sbjct: 243 PNGKVIDITPTPAA-LATFAFKKLTFSKKQLVPLL-MSFKSENLSYLINLIKEGKLKTVI 302

Query: 324 DSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           DSRYPLSKAE+AWAKSMDGHATGKIIVEP
Sbjct: 303 DSRYPLSKAEDAWAKSMDGHATGKIIVEP 329

BLAST of Lsi02G021150 vs. NCBI nr
Match: XP_022991688.1 (chloroplast envelope quinone oxidoreductase homolog [Cucurbita maxima])

HSP 1 Score: 561.2 bits (1445), Expect = 6.0e-156
Identity = 288/333 (86.49%), Postives = 302/333 (90.69%), Query Frame = 0

Query: 20  ASTTSSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR 79
           AS TS LMKAVLY+AYGGGSAALKH EVPIP PK+DEVLLKVEAVSLNPFDWKIQ GV+R
Sbjct: 2   ASATSRLMKAVLYEAYGGGSAALKHVEVPIPGPKDDEVLLKVEAVSLNPFDWKIQKGVVR 61

Query: 80  ------------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVC 139
                       TDVAGEVLEVGA VK LK GDKVVTFLNHATGGGLAEYVVAKE+STV 
Sbjct: 62  LFLPRNFPHIPCTDVAGEVLEVGARVKGLKAGDKVVTFLNHATGGGLAEYVVAKESSTVV 121

Query: 140 RPTEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKL 199
           RP EVTAAD AALPVAALTAH+ALTQAAGIKLD NGPQSNILITAASGGVGHYAVQLAKL
Sbjct: 122 RPPEVTAADGAALPVAALTAHQALTQAAGIKLDANGPQSNILITAASGGVGHYAVQLAKL 181

Query: 200 GNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFE 259
           GNTHVTATCGARN+EFVKSLGADEVIDY+TSEGA LKSPSGKKYDAVIHC TGIPWSVFE
Sbjct: 182 GNTHVTATCGARNIEFVKSLGADEVIDYKTSEGAALKSPSGKKYDAVIHCATGIPWSVFE 241

Query: 260 PNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKV 319
           PNLSS GKV+DITPANA+TV+TFA KKLTFSKKKLVP+W MSFK+ENL YVVNL+KEGKV
Sbjct: 242 PNLSSTGKVVDITPANASTVMTFAIKKLTFSKKKLVPMWSMSFKNENLGYVVNLMKEGKV 301

Query: 320 KSVIDSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           KS IDSRYPLSKAEEAWAKSMDGHATGKIIV+P
Sbjct: 302 KSTIDSRYPLSKAEEAWAKSMDGHATGKIIVQP 334

BLAST of Lsi02G021150 vs. NCBI nr
Match: XP_022953316.1 (chloroplast envelope quinone oxidoreductase homolog [Cucurbita moschata])

HSP 1 Score: 556.6 bits (1433), Expect = 1.5e-154
Identity = 286/333 (85.89%), Postives = 301/333 (90.39%), Query Frame = 0

Query: 20  ASTTSSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR 79
           ASTTS LMKAV Y+AYGGGSAALKH EVPIP PK+DEVLLKVEAVSLNPFDWKIQ GV+R
Sbjct: 2   ASTTSRLMKAVRYEAYGGGSAALKHVEVPIPGPKDDEVLLKVEAVSLNPFDWKIQKGVVR 61

Query: 80  ------------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVC 139
                       TDVAGEVLEVG+ VK LKVGDKVVTFLNHATGGGLAEYVVAKEASTV 
Sbjct: 62  PFLPRNFPHIPCTDVAGEVLEVGSRVKRLKVGDKVVTFLNHATGGGLAEYVVAKEASTVI 121

Query: 140 RPTEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKL 199
           RP EVTAAD AALPVAALTAH+ALTQAAGIKLD NGPQ NILITAASGGVGHYAVQLAKL
Sbjct: 122 RPPEVTAADGAALPVAALTAHQALTQAAGIKLDANGPQLNILITAASGGVGHYAVQLAKL 181

Query: 200 GNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFE 259
           GNTHVTATCGARN+EFVKSLGADEVIDY+TSEGA LKSPSGKKYDAVIHC TGIPWSVFE
Sbjct: 182 GNTHVTATCGARNIEFVKSLGADEVIDYKTSEGAALKSPSGKKYDAVIHCATGIPWSVFE 241

Query: 260 PNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKV 319
           PNLSSNGKV+DITPAN +TV+TFA KKLTFSKKKLVP+W MSFK+ENL YV+NL KEGKV
Sbjct: 242 PNLSSNGKVVDITPANVSTVMTFAIKKLTFSKKKLVPMWSMSFKNENLEYVMNLTKEGKV 301

Query: 320 KSVIDSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           KS+IDSRY LSKAEEAWAKS+DGHATGKIIVEP
Sbjct: 302 KSMIDSRYALSKAEEAWAKSVDGHATGKIIVEP 334

BLAST of Lsi02G021150 vs. NCBI nr
Match: KAG6576000.1 (Quinone-oxidoreductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 553.5 bits (1425), Expect = 1.3e-153
Identity = 285/333 (85.59%), Postives = 300/333 (90.09%), Query Frame = 0

Query: 20  ASTTSSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR 79
           ASTTS LMKAV Y+AYGGGSAALKH EVPIP PK+DEVLLKVEAVSLNPFDWKIQ GV+R
Sbjct: 2   ASTTSRLMKAVRYEAYGGGSAALKHVEVPIPGPKDDEVLLKVEAVSLNPFDWKIQKGVVR 61

Query: 80  ------------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVC 139
                       TDVAGEVLEVG+ VK LK GDKVVTFLNHATGGGLAEYVVAKEASTV 
Sbjct: 62  PFLPRNFPHIPCTDVAGEVLEVGSRVKRLKAGDKVVTFLNHATGGGLAEYVVAKEASTVI 121

Query: 140 RPTEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKL 199
           RP EVTAAD AALPVAALTAH+ALTQAAGIKLD NGPQ NILITAASGGVGHYAVQLAKL
Sbjct: 122 RPPEVTAADGAALPVAALTAHQALTQAAGIKLDANGPQLNILITAASGGVGHYAVQLAKL 181

Query: 200 GNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFE 259
           GNTHVTATCGARN+EFVKSLGADEVIDY+TSEGA LKSPSGKKYDAVIHC TGIPWSVFE
Sbjct: 182 GNTHVTATCGARNIEFVKSLGADEVIDYKTSEGAALKSPSGKKYDAVIHCATGIPWSVFE 241

Query: 260 PNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKV 319
           PNLSSNGKV+DITPANA+TV+TFA KKLTFSKKKLVP+W MSFK+ENL YV+NL KE KV
Sbjct: 242 PNLSSNGKVIDITPANASTVMTFAIKKLTFSKKKLVPMWSMSFKNENLEYVMNLTKERKV 301

Query: 320 KSVIDSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           KS+IDSRY LSKAEEAWAKS+DGHATGKIIVEP
Sbjct: 302 KSMIDSRYTLSKAEEAWAKSVDGHATGKIIVEP 334

BLAST of Lsi02G021150 vs. NCBI nr
Match: XP_023549129.1 (chloroplast envelope quinone oxidoreductase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 551.6 bits (1420), Expect = 4.8e-153
Identity = 284/333 (85.29%), Postives = 300/333 (90.09%), Query Frame = 0

Query: 20  ASTTSSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR 79
           ASTTS LMKAV Y+AYGGGSAALKH EVPIP PK+DEVLLKVEAVSLNPFDWKIQ GV+R
Sbjct: 2   ASTTSRLMKAVRYEAYGGGSAALKHVEVPIPGPKDDEVLLKVEAVSLNPFDWKIQKGVVR 61

Query: 80  ------------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVC 139
                       TDV GEVLEVG+ VK LKVGDKVVTFLNHATGGGLAEYVVAKEASTV 
Sbjct: 62  PFLPRNFPHIPCTDVTGEVLEVGSRVKRLKVGDKVVTFLNHATGGGLAEYVVAKEASTVI 121

Query: 140 RPTEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKL 199
           RP EVTAAD AAL VAALTAH+ALTQAAGIKLD NGPQSNILITAASGGVGHYAVQLAKL
Sbjct: 122 RPPEVTAADGAALTVAALTAHQALTQAAGIKLDGNGPQSNILITAASGGVGHYAVQLAKL 181

Query: 200 GNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFE 259
           GNTHVTATCGARN+EFVKSLGADEVIDY+T EGA LKSPSGKKYDAVIHC TGIPWSVFE
Sbjct: 182 GNTHVTATCGARNIEFVKSLGADEVIDYKTLEGAALKSPSGKKYDAVIHCATGIPWSVFE 241

Query: 260 PNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKV 319
            NLSSNGKV+DITPANA+TV+TFA KK TFSKKKLVP+W MSFK+ENL YVVNL+KEGKV
Sbjct: 242 RNLSSNGKVVDITPANASTVMTFAIKKFTFSKKKLVPMWSMSFKNENLEYVVNLIKEGKV 301

Query: 320 KSVIDSRYPLSKAEEAWAKSMDGHATGKIIVEP 341
           KS+IDSRYPLS+AEEAWAKSM+GHATGKIIVEP
Sbjct: 302 KSMIDSRYPLSQAEEAWAKSMNGHATGKIIVEP 334

BLAST of Lsi02G021150 vs. NCBI nr
Match: KAG7014521.1 (Quinone-oxidoreductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 524.6 bits (1350), Expect = 6.2e-145
Identity = 274/330 (83.03%), Postives = 289/330 (87.58%), Query Frame = 0

Query: 20  ASTTSSLMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR 79
           ASTTS LMKAV Y+AYGGGSAALKH EVPIP PK+DEVLLKVEAVSLNPFDWKIQ GV+R
Sbjct: 2   ASTTSRLMKAVRYEAYGGGSAALKHVEVPIPGPKDDEVLLKVEAVSLNPFDWKIQKGVVR 61

Query: 80  ------------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVC 139
                       TDVAGEVLEVG+ VK LK GDKV       TGGGLAEYVVAKEASTV 
Sbjct: 62  PFLPRNFPHIPCTDVAGEVLEVGSRVKRLKAGDKV-------TGGGLAEYVVAKEASTVI 121

Query: 140 RPTEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKL 199
           RP EVTAAD AALPVAALTAH+ALTQAAGIKLD NGPQ NILITAASGGVGHYAVQLAKL
Sbjct: 122 RPPEVTAADGAALPVAALTAHQALTQAAGIKLDANGPQLNILITAASGGVGHYAVQLAKL 181

Query: 200 GNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFE 259
           GNTHVTATCGARN+EFVKSLGADEVIDY+TSEGA LKSPSGKKYDAVIHC T IPWSVFE
Sbjct: 182 GNTHVTATCGARNIEFVKSLGADEVIDYKTSEGAALKSPSGKKYDAVIHCATRIPWSVFE 241

Query: 260 PNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKV 319
           PNLSSNGKV+DITPANA+TV+TF  KKLTFSKKKLVP+W MSFK+ENL YV+NL KEGKV
Sbjct: 242 PNLSSNGKVIDITPANASTVMTFVIKKLTFSKKKLVPMWSMSFKNENLEYVMNLTKEGKV 301

Query: 320 KSVIDSRYPLSKAEEAWAKSMDGHATGKII 338
           KS+IDSRY LSKAEEAWAKS+DGHATGKII
Sbjct: 302 KSMIDSRYTLSKAEEAWAKSVDGHATGKII 324

BLAST of Lsi02G021150 vs. TAIR 10
Match: AT4G13010.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 430.3 bits (1105), Expect = 1.5e-120
Identity = 224/327 (68.50%), Postives = 265/327 (81.04%), Query Frame = 0

Query: 26  LMKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIR------ 85
           LM A+ Y +YGGG+A L+H +VP+P PK++EV LK+EA SLNP DWKIQ G+IR      
Sbjct: 5   LMHALQYNSYGGGAAGLEHVQVPVPTPKSNEVCLKLEATSLNPVDWKIQKGMIRPFLPRK 64

Query: 86  ------TDVAGEVLEVGAEVKSLKVGDKVVTFLNHATGGGLAEYVVAKEASTVCRPTEVT 145
                 TDVAGEV+EVG+ VK+ K GDKVV  L+H  GGGLAE+ VA E  TV RP EV 
Sbjct: 65  FPCIPATDVAGEVVEVGSGVKNFKAGDKVVAVLSHLGGGGLAEFAVATEKLTVKRPQEVG 124

Query: 146 AADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKLGNTHVT 205
           AA++AALPVA LTA +ALT  AG+KLD  G ++NIL+TAASGGVGHYAVQLAKL N HVT
Sbjct: 125 AAEAAALPVAGLTALQALTNPAGLKLDGTGKKANILVTAASGGVGHYAVQLAKLANAHVT 184

Query: 206 ATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFEPNLSSN 265
           ATCGARN+EFVKSLGADEV+DY+T EGA LKSPSGKKYDAV+HC  GIP+SVFEPNLS N
Sbjct: 185 ATCGARNIEFVKSLGADEVLDYKTPEGAALKSPSGKKYDAVVHCANGIPFSVFEPNLSEN 244

Query: 266 GKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSVIDS 325
           GKV+DITP   A + T+A KK+T SKK+LVPL  +  K+ENL ++VNLVKEGKVK+VIDS
Sbjct: 245 GKVIDITPGPNA-MWTYAVKKITMSKKQLVPLL-LIPKAENLEFMVNLVKEGKVKTVIDS 304

Query: 326 RYPLSKAEEAWAKSMDGHATGKIIVEP 341
           ++PLSKAE+AWAKS+DGHATGKIIVEP
Sbjct: 305 KHPLSKAEDAWAKSIDGHATGKIIVEP 329

BLAST of Lsi02G021150 vs. TAIR 10
Match: AT1G23740.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 132.5 bits (332), Expect = 6.4e-31
Identity = 105/335 (31.34%), Postives = 150/335 (44.78%), Query Frame = 0

Query: 27  MKAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNGVIRT------ 86
           MKA +Y  YGG       + + +P  K D+VL+KV A +LNP D K + G  +       
Sbjct: 78  MKAWVYSDYGGVDVLKLESNIVVPEIKEDQVLIKVVAAALNPVDAKRRQGKFKATDSPLP 137

Query: 87  -----DVAGEVLEVGAEVKSLKVGDKVVTFLNHAT------GGGLAEYVVAKEASTVCRP 146
                DVAG V++VG+ VK LK GD+V   ++          G LAEY   +E     +P
Sbjct: 138 TVPGYDVAGVVVKVGSAVKDLKEGDEVYANVSEKALEGPKQFGSLAEYTAVEEKLLALKP 197

Query: 147 TEVTAADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAK--L 206
             +  A +A LP+A  TA E L +             +IL+   +GGVG   +QLAK   
Sbjct: 198 KNIDFAQAAGLPLAIETADEGLVRT------EFSAGKSILVLNGAGGVGSLVIQLAKHVY 257

Query: 207 GNTHVTATCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFE 266
           G + V AT     +E V+SLGAD  IDY       L       +DA+  C   +      
Sbjct: 258 GASKVAATASTEKLELVRSLGADLAIDYTKENIEDLPDKYDVVFDAIGMCDKAV------ 317

Query: 267 PNLSSNGKVMDITPANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKV 326
             +   GKV+ +T A       F         KKL P                 ++ GKV
Sbjct: 318 KVIKEGGKVVALTGAVTPPGFRFVVTSNGDVLKKLNP----------------YIESGKV 377

Query: 327 KSVIDSR--YPLSKAEEAWAKSMDGHATGKIIVEP 341
           K V+D +  +P S+  +A++     HATGK++V P
Sbjct: 378 KPVVDPKGPFPFSRVADAFSYLETNHATGKVVVYP 384

BLAST of Lsi02G021150 vs. TAIR 10
Match: AT3G15090.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 119.4 bits (298), Expect = 5.6e-27
Identity = 95/341 (27.86%), Postives = 153/341 (44.87%), Query Frame = 0

Query: 28  KAVLYKAYGGGSAALKHAEVPIPRPKNDEVLLKVEAVSLNPFDWKIQNG----------- 87
           +AV+   +GG         VP+P    +EVL+K +AVS+NP D +I+ G           
Sbjct: 33  RAVILPRFGGPEVFELRENVPVPNLNPNEVLVKAKAVSVNPLDCRIRAGYGRSVFQPHLP 92

Query: 88  -VIRTDVAGEVLEVGAEVKSLKVGDKVVTFLN-HATGGGLAEYVVAKEASTVCRPTEVTA 147
            ++  DV+GEV  +G  VKSLKVG +V   L+  A  G   +Y +  E     +P+ ++ 
Sbjct: 93  IIVGRDVSGEVAAIGTSVKSLKVGQEVFGALHPTALRGTYTDYGILSEDELTEKPSSISH 152

Query: 148 ADSAALPVAALTAHEALTQAAGIKLDRNGPQSNILITAASGGVGHYAVQLAKLGNTHVTA 207
            +++A+P AALTA  AL   A I          +L+    G VG  A+QLA     HVTA
Sbjct: 153 VEASAIPFAALTAWRALKSNARIT-----EGQRLLVFGGGGAVGFSAIQLAVASGCHVTA 212

Query: 208 TCGARNVEFVKSLGADEVIDYRTSEGATLKSPSGKKYDAVIHCTTGIPWSVFEPN-LSSN 267
           +C  +  + + + GA++ +DY T +   ++     K+DAV+    G        N L   
Sbjct: 213 SCVGQTKDRILAAGAEQAVDYTTED---IELAVKGKFDAVLDTIGGPETERIGINFLRKG 272

Query: 268 GKVMDITPANAATVLTFAF-------------KKLTFSKKKLVPLWG--MSFKSENLRYV 327
           G  M +    A+    + F             KK+ +     +  W   M    E L  +
Sbjct: 273 GNYMTLQGEAASLTDKYGFVVGLPLATSLLMKKKIQYQYSHGIDYWWTYMRADPEGLAEI 332

Query: 328 VNLVKEGKVKSVIDSRYPLSKAEEAWAKSMDGHATGKIIVE 340
             LV  GK+K  ++  +P++    A          GK+++E
Sbjct: 333 QRLVGAGKLKIPVEKTFPITDVVAAHEAKEKKQIPGKVVLE 365

BLAST of Lsi02G021150 vs. TAIR 10
Match: AT1G49670.1 (ARP protein (REF) )

HSP 1 Score: 77.4 bits (189), Expect = 2.5e-14
Identity = 88/317 (27.76%), Postives = 134/317 (42.27%), Query Frame = 0

Query: 56  EVLLKVEAVSLNPFDWKIQNGVIRT------------DVAGEVLEVGAEVKSLKVGDKVV 115
           +VLLK+    +N  D    +G   T            +  G +  VG  VK+L+VG    
Sbjct: 318 QVLLKIIYAGVNASDVNFSSGRYFTGGSPKLPFDAGFEGVGLIAAVGESVKNLEVGTPAA 377

Query: 116 TFLNHATGGGLAEYVV--AKEASTVCRPTEVTAADSAALPVAALTAHEALTQAAGIKLDR 175
                 T G  +EY++  +K    V RP      +  A+  + LTA  AL +A  +K   
Sbjct: 378 VM----TFGAYSEYMIVSSKHVLPVPRPD----PEVVAMLTSGLTALIALEKAGQMK--- 437

Query: 176 NGPQSNILITAASGGVGHYAVQLAKLGNTHVTATC-GARNVEFVKSLGADEVIDYRTSEG 235
                 +L+TAA+GG G +AVQLAKL    V ATC G+   + +K LG D VIDY++   
Sbjct: 438 --SGETVLVTAAAGGTGQFAVQLAKLSGNKVIATCGGSEKAKLLKELGVDRVIDYKSENI 497

Query: 236 AT-LKSPSGKKYDAVIHCTTGIPWSVFEPNLSSNGKVMDI----------------TPAN 295
            T LK    K  + +     G  + +    L+  G+++ I                 P  
Sbjct: 498 KTVLKKEFPKGVNIIYESVGGQMFDMCLNALAVYGRLIVIGMISQYQGEKGWEPAKYPGL 557

Query: 296 AATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSVIDSR--YPLSKAE 339
              +L  +     F   +   LW      +NL  + NL   GK+K  ID +    L+   
Sbjct: 558 CEKILAKSQTVAGFFLVQYSQLW-----KQNLDKLFNLYALGKLKVGIDQKKFIGLNAVA 616

BLAST of Lsi02G021150 vs. TAIR 10
Match: AT3G56460.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.6e-13
Identity = 83/317 (26.18%), Postives = 138/317 (43.53%), Query Frame = 0

Query: 48  PIPRPKND-EVLLKVEAVSLNPFDW-----KIQNG-----VIRTDVAGEVLEVGAEVKSL 107
           PIP   +D  V ++V A SLN  ++     K Q       +  +D +G V  +G  V   
Sbjct: 31  PIPSLNSDTSVRVRVIATSLNYANYLQILGKYQEKPPLPFIPGSDYSGIVDAIGPAVTKF 90

Query: 108 KVGDKVVTFLNHATGGGLAEYVVAKEASTVCRPTEVTAADSAALPVAALTAHEALTQAAG 167
           +VGD+V +F   A  G  A+++VA ++     P       +AALPVA  T+H AL   A 
Sbjct: 91  RVGDRVCSF---ADLGSFAQFIVADQSRLFLVPERCDMVAAAALPVAFGTSHVALVHRA- 150

Query: 168 IKLDRNGPQSNILITAASGGVGHYAVQLAKLGNTHVTATC-GARNVEFVKSLGADEVIDY 227
               R      +L+  A+GGVG  AVQ+ K+    V A   G   ++ +KS+G D V+D 
Sbjct: 151 ----RLTSGQVLLVLGAAGGVGLAAVQIGKVCGAIVIAVARGTEKIQLLKSMGVDHVVDL 210

Query: 228 RT-----SEGATLKSPSGKKYDAVIHCTTG---------IPWSVFEPNLSSNGKVMDITP 287
            T     S    +K+   K  D +     G         + W      +      + + P
Sbjct: 211 GTENVISSVKEFIKTRKLKGVDVLYDPVGGKLTKESMKVLKWGAQILVIGFASGEIPVIP 270

Query: 288 ANAATVLTFAFKKLTFSKKKLVPLWGMSFKSENLRYVVNLVKEGKVKSVIDSRYPLSKAE 339
           AN A V  +    L +   +   +   +   ++++ +++ +  G +   I   Y LS+A 
Sbjct: 271 ANIALVKNWTVHGLYWGSYR---IHQPNVLEDSIKELLSWLSRGLITIHISHTYSLSQAN 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SV682.1e-11968.50Chloroplast envelope quinone oxidoreductase homolog OS=Arabidopsis thaliana OX=3... [more]
Q8H0M18.8e-11866.87Quinone-oxidoreductase homolog, chloroplastic OS=Spinacia oleracea OX=3562 GN=QO... [more]
Q9AYU11.7e-11365.44Quinone-oxidoreductase QR1, chloroplastic OS=Triphysaria versicolor OX=64093 PE=... [more]
Q84V253.1e-3032.082-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=1[more]
Q9ZUC19.0e-3031.34NADPH-dependent alkenal/one oxidoreductase, chloroplastic OS=Arabidopsis thalian... [more]
Match NameE-valueIdentityDescription
A0A6J1JX052.9e-15686.49chloroplast envelope quinone oxidoreductase homolog OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1GMN07.2e-15585.89chloroplast envelope quinone oxidoreductase homolog OS=Cucurbita moschata OX=366... [more]
A0A0A0K8E01.4e-14281.21PKS_ER domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G351910 PE=4... [more]
A0A1S3C6G67.0e-14280.55putative quinone-oxidoreductase homolog, chloroplastic OS=Cucumis melo OX=3656 G... [more]
A0A5A7SP071.2e-14180.55Putative quinone-oxidoreductase-like protein OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
XP_022991688.16.0e-15686.49chloroplast envelope quinone oxidoreductase homolog [Cucurbita maxima][more]
XP_022953316.11.5e-15485.89chloroplast envelope quinone oxidoreductase homolog [Cucurbita moschata][more]
KAG6576000.11.3e-15385.59Quinone-oxidoreductase-like, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_023549129.14.8e-15385.29chloroplast envelope quinone oxidoreductase homolog [Cucurbita pepo subsp. pepo][more]
KAG7014521.16.2e-14583.03Quinone-oxidoreductase-like, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
AT4G13010.11.5e-12068.50Oxidoreductase, zinc-binding dehydrogenase family protein [more]
AT1G23740.16.4e-3131.34Oxidoreductase, zinc-binding dehydrogenase family protein [more]
AT3G15090.15.6e-2727.86GroES-like zinc-binding alcohol dehydrogenase family protein [more]
AT1G49670.12.5e-1427.76ARP protein (REF) [more]
AT3G56460.11.6e-1326.18GroES-like zinc-binding alcohol dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020843Polyketide synthase, enoylreductase domainSMARTSM00829PKS_ER_names_modcoord: 37..338
e-value: 4.2E-11
score: -1.1
IPR013154Alcohol dehydrogenase, N-terminalPFAMPF08240ADH_Ncoord: 55..129
e-value: 1.4E-4
score: 21.7
NoneNo IPR availableGENE3D3.40.50.720coord: 151..291
e-value: 3.9E-94
score: 317.2
NoneNo IPR availablePFAMPF13602ADH_zinc_N_2coord: 207..338
e-value: 2.0E-20
score: 74.2
NoneNo IPR availableGENE3D3.90.180.10coord: 34..338
e-value: 3.9E-94
score: 317.2
NoneNo IPR availablePANTHERPTHR44013:SF12ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C16A3.02C-RELATEDcoord: 17..340
NoneNo IPR availablePANTHERPTHR44013ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C16A3.02Ccoord: 17..340
NoneNo IPR availableCDDcd08267MDR1coord: 30..338
e-value: 3.52288E-112
score: 326.865
IPR011032GroES-like superfamilySUPERFAMILY50129GroES-likecoord: 26..156
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 133..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G021150.1Lsi02G021150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity