Lsi02G020800 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G020800
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	E3 ubiquitin-protein ligase PRT1
Location	chr02: 26990620 .. 26998618 (+)
RNA-Seq Expression	Lsi02G020800
Synteny	Lsi02G020800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCGTGTCTTGTTTTGTTCTGATGTTCTATCGATGAGGTTAAGAATTTGAATATTCAATTATTCTTGATGATTTCTTGTTTTGGGTCATCTTCGATTCGCGATGTGCCCATATGTCATAGCTATACTGTGTATTTTACATGTTCAATGTATATCCCCTTAGGAAGAGGTAAGGCTTTCAGGAAGGTTATCCATATCAAATGTTGTTTTTCTCAAAATTCCACTTTGGGAACCCTAGTATTTTTTCTGTAAACTTGAATTTAGTTATGGTCCATAGCCTTATTCCTCGTTTGGCCCAAAAGCCACCCAGGTGGGGTTGATAAATTACAGAATTGAACAAAATGTACTGGTCGTTGTCTTTTCATTTTGTCCAAGCATTGTGCTAGATATTGGATCCAAAATAAACACTCAATGTAAATTGTTGAGTTCGGGTTGACTGTTTTTCAGTTACTCTTTCAATAACTCGATATCAGGGTCCCGCCCCCGATTAATCTGGAAACTTATGCCTACCCTAAGGCTCAGATTGAGAGACATCAAATATTTTTCTTCTTATTAAGCTCACACAAAGGTTTGAACACTCACTGAAGTCATTGTAGTTGACTTGATGTTGACTATTCTTTAGCTATTTGAAATCATTGATGACATCATAGAGTGGGACATTGCCTTTGGAATTTGTTCATGCTCAATTCTATGATAAATTGTAACCATCATGGGTTGGCCTAGTCTCAATAAATGAGATATAGTCTCAATAAATGGCTAAGAGGTTCGGGATTCAATCCATGGTGGCCACCTACCGAGGAAAGAGTTTCTTTGACACCCAAATGTTGTAGGATTAAGCGGGTTGTTCTGTGAAATTAGTCGAGGTGCGCGTAAGCTAGTCTGGACACTCACAGATATTTAAAGAAAAAAAAACAATTCCATGATAAATTGTAGGAATGACTTCTAAGAACTAAAGAATGGTCAATAACAAGCATTGACAAGTTTTCTTCTGATAAGCTTTTTGCACTTCTCCAAATAGTGGAGGTCCTTTTCTATTGCCTACTTTTTTGTTGTCCCTGAATTATGCCAAATAGATGTTTATAAAGTAAGCAATTGGTATGGCAGGCGTGGTTGGAAACAAAAAATATTATTTTGTTAGTATAACTTCTTACAAACGCATATCCAATAGGACCAAAGCTTAGACTGGGTTTTCTGGGATTTTGGAGTAAAGTTAAACTCTGAATTGAGCATTACGTGATCATGAGGTAATGACTTATAGTATTATTAGAAATGAGAAACAAAAACTGGGACATTTGCACGGAGGTTTTTTTATCTGTTGATACTATTATTTGTCTTCAGCAGTTCCTTCTTACATGTGGCTAATTTAAGTTGGTTGTGAATTGCAGGGATCTTCTCCACAAGCCGATAGTGCTCTGTGAGTATAATTAGATGTTGACCATAAATTTTTTTTTGGAGGTGTCATCCTTTTGTTAATTTTTGGTTTCTTCTATTCCAGCTTGTGGTCATATTTCATGTTTTTGGTGCGTGCACAAATCCATGAATGGTTTTCATGAGTCCCACTGTCCAATGTGTAGGCGTCCATATTATCACTTTCCAACCATCTGTGAGATGCTTCACCAATTGATCCTGAAGATTTATCCTGCAGCGTATAAGAAAAGGGAAAATCAAATACTAGGTGAGCTTTCTATTGTATGCTGTTATCACTAATGTGCTAATGTCTGCAATGGTTGCAATCATGGCTGTTAAAAACAAACATCTCAAGCTGATGCATATCGTATGAGCTGAGGTGTAATGGTTTTAGCCGTGAATCTGTTAGGTTTCTACTCCACCTGAACTTAGTAAAACCCAACAAAGAATAGAGCAAAAAACAGAGGAAGTTGATCCTGTTTCGATGATATATTCAAATGGGATTTCCACCACAAGTTCTCAACGATATCAAAGTTGCTAAGCCAAAATAACACAAGAAATGACAATGAGAATAGAAAAGAGAAGAACAATTGCAAAGCACTCCAACACTTTCGAGAGAGCTGGAAATCCTCTCCAAACTTTTAGCCTTCAGCCAAATTCTTCTCTGCCTCACATTGAAACCCAAAACCCTACAAAAACCCTCCAAGTGACCCCCACCTCCATCGAACAATGAAAAACGTCCCCTCTGCATTTCCTGTGCATTTCCCACTTGTCCCCTGTGCATTTCCTCTATTACCCCTTAAGTATGTGTAGAGGATGATGGTCTGTTCACTCAATGAAAACCGTGAATTATTGAGGACTCAAATGATTGATCTAGCTTATTGGAAGATGGAAACAAAGGCACATAAATTGTACTTATTATCTTTACTACCTGGTGAATTTACTTATTCTTTAACCTACATAACTCATGAGAGTGCTGCAAGAAAATGAGGTAGGGAGTGCAATTAATGATGGGGAAGAAATATAAACAATTGAAGAAGCATTGACCTGATGGTCGATCCAGTAGGAGCTGTTACTTTTTGAAGAAGGAAGAATATAGAACAGTGGGATTGAAATAAAAATAAACGCATAGGAGTCAATGTCAAAACTCAATAATATTTGCCATCTTGACCACAGATGGGAGTGGAATGAATGTACATAGATTTTTGGTTCAGCAAATGAATAATTATTCATTGTTTTTCTTGGAGGTTTTTGGTAGAGTTATTTTAACCTTTTACTTTATTTCTATACCTTTCTACTTCACTGTTTTATACATTTAAATAATTTTCATTTTCAGAAGAGGAAAGGAAAATTGGCCTTTTCTCTCCACAATTTGATTCCCTTGCATGTGGCTCCCAAGCTGGTATAAAAGTGGAACACTTAGAAGATTCTGCTAACGGCAAGCTGAACACAATTACAAAAAATGACGACGTTGTAACTGAATTAGTATTAAAAGAAAATTCTGAAGTTGTTAGATCTACTTCTGTTGAAAGCCTCAATTCTCTTCAAGATCCTTGTACTCAAAAGACTCTAAACCAGGAGAAGATTTCAGTTGCTGATGTGCTATGTCGAGGGTGCACCCAATTGCTCTTTCGACCTGTGGTTCTTAACTGTGGCCATGGTATGTATGATCTTTTTTCATCCGTGTGAGCTTGGATTCTATTTTATCCTCAACTATGTAGCAAAAGTATTTGTTAAGTATCGGGGTTATTTCAATTTTCAACTTTAATACCATTCCCAAAATATCTCGACTTCAATGCCAAAAACAAAAAGGTTTCAATAAAGAACTTATTTTCTTATGTTAACAGCATTTTGTGAGAGTTGTATCAACTCGCAAGTTGAGACACTTGAGTGTCAAGTTTGTCAGTTTCTGCAACCAAGGGGATTTCCAAATGTCTGTTTGGAGCTTGATCAGTTTTTGAAGGAACAATTTCCTGAAGAATATAGCCTCCGAAGAGACTCCGTTCAATTTGAACTAGCCAAAAGTATCAAGCATGACATTCCAACTTCCTGTACGTTTATTTACACTTGCTTAATTAATCATTTTTAATATTGTGGCATTTGTGTTCTTGGATTAATCTATTTGTGGTGCTTTTAGTGCTTGCTATTTGTTTCATTCTTCTCTTTTTTCTTTCTTAATTTATAGTTCACGTATATATTGCACTTTTGTTTGTGCTCAGCTTGTGCGGACTGAAGGTAGTTTAGCTGTCCAAACTATATAATTGTTAGAGGTTGTAGGAAAATTGCTTTGTGAATCATTTTGAATAGCATGTATCGATTTATTTCTGATATTTCTATATAATCTTTGAACTGATGAAATTTACTTTCTTTCAAAGTTTACCTGTGTTTTTAGGTCCATTTATTTGATAGCAGAGCTGTTCAACTACCATCCATGCTTTATTCATTTCTTTCATATTTTGGTAATTTCAGGAAAACAAAATACTGGACACATGTAGGAATTTTAAAATGATGGTGTTATTTACAATTTTATTCTTCAATTCTTTGAAAAGGGTAGACATGTACTTATAATACTTGATGGCGTATTGTTGCTCTACAATATTGTAAATGATTCATTAATACAAAACTAGTCTGTATAATATCAGTTTGGTTTATTTACGTTTATTATTTGCTTCCAGGTTCCAATGAACAGGGTAAGAAAGGAAAGGATCTACCAATATGGGGTGAAGTTGCTTCTAAGGTGCATTCTGCAGTTGGCTGTGATTCTTGTGGGGTAATTTCCTCATATTTTCACCATTCTTATCACGCTGTCAATGTTTTAAAAGGCTATTTCGTGTAGTCTAAAGCATAAAAGGAGAGAAATTTCCAATAATAAAATCAAAGTTTTTATTGTGAATCAAAAGTTATCAATACAAGAAGACAATCCATTTATTTATAGAGAATTGGAAGACAAATTAATCCTAATCCTAATTAATCAAAGAAACTAATGTTAATCTTAATAAACTAAGGAAACTAATCCTTATCCTAATCAATCAAGAAAACTTATCATAATCCTAATCAATTAAGGAAACTTATCCTAATCCTAATCAATTAAGGATTTGACCAAGATACCCTAATTTACTCTAATTTACTACATCACTATTGAGGCTTACACATTGATGCTCGGCTTGAACAAAACTCTAGATTTTACCCTCGACATTTACTCCTCTTTGAAGAAGCTTACGTCTATGTGAAAATGCATTGAGGCCTAAAATCTTTAGCCTTGAATAATGATTAGATAGATAATATTTATTTGCTTCATTTTTCCATTTAAATTGTGACAATTTGAACTATTACTACTTTTGCTAGTGTGACTTTAAAAGGATTGAAGATACAATTTACAGTAAAATTCAATTCTACTAATGATAGTAGTGAGGTTAAAGATATCCTTTTAGAATTTTAAGTGGCAATTGACAGGTTTTTGGTTTAGGGTATTTTTTTTTTGTTAGTTTTTTGTTTTTTAATAATAGTTTTATATCACTATTATGTTGAACATTACTTGGTCATGCCAATTTAAGATTTCAAGAATATTTTAATCAAATTTGAAGCTTACACCTCATGCTGCCTCACCTCTTTAGGAGGAAAACCTCAAGGCTGCCTTTAGCCTTTTCAAATCTTGCATATTGTTTGAGATCTTTGTTGTTGAACTGTAAAAGATAGAATCCAAACAAAAAATGGAATAATAAAAGATAGAAGAACTGCAATATATCCCTAATTATTCAACTGTGATGCTTGGGTTGAGAATATCGCATTGAAGCTTGACTATAAAGTGGGTCATTGGCCAACTATTTACATGGGGCTTCCTTTATATGGTAAGTCATCACCAATATCCTTTTGGCAGCCTATTTTGGACAAGATTGATAAGAGTCGACACTCATGGCAGGACAACCACCTCTCCAAAGGTGGTAGATCAACTTTTGAATGCTACATTAGCCAATCAGCCTATAGATTTCTGTTAGGTACTTAAGCACCTTAGAGAACCACACCCCAAAAGCTAGCTATTGAGGTGGGAGAGCCAAGCCACTTAAGTACCATATTGGTCATACCATTCTAACCAATGTGGGACAAAGGTAGCCCATACTACCTTGGTTCCTAACAATACCCCATCCCTGGAAAGCCGACGTCCCGGCGGCTACTCTGACGGTACTTCACTCGGCCACACCCTAGCTTTTAGGGTGTGGTTCTCCAAGGTGCTTAAGTACCTAACAATGGTATCAGAGCCGGTTGTTGACGTTTCGGAGTGGAACCGGCGAAGGGTTCTGACCGGGTGTGGCCGAGTGAAGTACCGTCGGAGTAGCCAACGTCCGGAGATGGGGTATTGTTAGGAACCAAGGTAGTATGGGCTACCTTTGTCCCACATTGGTTAGAATGGGATGACCAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGCCGCGCAGCGCCAACGCCTAGCGCGCGCGCCTGTCCCTCCGCGCGCGCCTCATCGTCCAGACCCCTCTGGACAGTCCTGGAAGCTTCTAGAAGGCTCGGGACAATGCCAGCGCCCACGCCCAAGACCCAGCAGCGCCTGGAAGGATCTGGGCGGCGCTGGACAGTGCCAGCGCCCACGAGAAAGCCCCAGCATGGCCCAGAAGGCTCCGGATGCCTCTGGACAGCGCCAGAACGTGCGCCACTGCCCTGGAAATGCCTGGAAGGATCTAGAAGGGGCGAGATAG

mRNA sequence

ATGTGCGGATCTTCTCCACAAGCCGATACTTGTGGTCATATTTCATGTTTTTGGTGCGTGCACAAATCCATGAATGGTTTTCATGAGTCCCACTGTCCAATGTGTAGGCGTCCATATTATCACTTTCCAACCATCTGTGAGATGCTTCACCAATTGATCCTGAAGATTTATCCTGCAGCGTATAAGAAAAGGGAAAATCAAATACTAGAAGAGGAAAGGAAAATTGGCCTTTTCTCTCCACAATTTGATTCCCTTGCATGTGGCTCCCAAGCTGGTATAAAAGTGGAACACTTAGAAGATTCTGCTAACGGCAAGCTGAACACAATTACAAAAAATGACGACGTTGTAACTGAATTAGTATTAAAAGAAAATTCTGAAGTTGTTAGATCTACTTCTGTTGAAAGCCTCAATTCTCTTCAAGATCCTTGTACTCAAAAGACTCTAAACCAGGAGAAGATTTCAGTTGCTGATGTGCTATGTCGAGGGTGCACCCAATTGCTCTTTCGACCTGTGGTTCTTAACTGTGGCCATGCATTTTGTGAGAGTTGTATCAACTCGCAAGTTGAGACACTTGAGTGTCAAGTTTGTCAGTTTCTGCAACCAAGGGGATTTCCAAATGTCTGTTTGGAGCTTGATCAGTTTTTGAAGGAACAATTTCCTGAAGAATATAGCCTCCGAAGAGACTCCGTTCAATTTGAACTAGCCAAAAGTATCAAGCATGACATTCCAACTTCCTGTTCCAATGAACAGGGTAAGAAAGGAAAGGATCTACCAATATGGGGTGAAGTTGCTTCTAAGGTGCATTCTGCAGTTGGCTGTGATTCTTGTGGGCGCCAACGCCTAGCGCGCGCGCCTGTCCCTCCGCGCGCGCCTCATCGTCCAGACCCCTCTGGACAGTCCTGGAAGCTTCTAGAAGGCTCGGGACAATGCCAGCGCCCACGCCCAAGACCCAGCAGCGCCTGGAAGGATCTGGGCGGCGCTGGACAGTGCCAGCGCCCACGAGAAAGCCCCAGCATGGCCCAGAAGGCTCCGGATGCCTCTGGACAGCGCCAGAACGTGCGCCACTGCCCTGGAAATGCCTGGAAGGATCTAGAAGGGGCGAGATAG

Coding sequence (CDS)

ATGTGCGGATCTTCTCCACAAGCCGATACTTGTGGTCATATTTCATGTTTTTGGTGCGTGCACAAATCCATGAATGGTTTTCATGAGTCCCACTGTCCAATGTGTAGGCGTCCATATTATCACTTTCCAACCATCTGTGAGATGCTTCACCAATTGATCCTGAAGATTTATCCTGCAGCGTATAAGAAAAGGGAAAATCAAATACTAGAAGAGGAAAGGAAAATTGGCCTTTTCTCTCCACAATTTGATTCCCTTGCATGTGGCTCCCAAGCTGGTATAAAAGTGGAACACTTAGAAGATTCTGCTAACGGCAAGCTGAACACAATTACAAAAAATGACGACGTTGTAACTGAATTAGTATTAAAAGAAAATTCTGAAGTTGTTAGATCTACTTCTGTTGAAAGCCTCAATTCTCTTCAAGATCCTTGTACTCAAAAGACTCTAAACCAGGAGAAGATTTCAGTTGCTGATGTGCTATGTCGAGGGTGCACCCAATTGCTCTTTCGACCTGTGGTTCTTAACTGTGGCCATGCATTTTGTGAGAGTTGTATCAACTCGCAAGTTGAGACACTTGAGTGTCAAGTTTGTCAGTTTCTGCAACCAAGGGGATTTCCAAATGTCTGTTTGGAGCTTGATCAGTTTTTGAAGGAACAATTTCCTGAAGAATATAGCCTCCGAAGAGACTCCGTTCAATTTGAACTAGCCAAAAGTATCAAGCATGACATTCCAACTTCCTGTTCCAATGAACAGGGTAAGAAAGGAAAGGATCTACCAATATGGGGTGAAGTTGCTTCTAAGGTGCATTCTGCAGTTGGCTGTGATTCTTGTGGGCGCCAACGCCTAGCGCGCGCGCCTGTCCCTCCGCGCGCGCCTCATCGTCCAGACCCCTCTGGACAGTCCTGGAAGCTTCTAGAAGGCTCGGGACAATGCCAGCGCCCACGCCCAAGACCCAGCAGCGCCTGGAAGGATCTGGGCGGCGCTGGACAGTGCCAGCGCCCACGAGAAAGCCCCAGCATGGCCCAGAAGGCTCCGGATGCCTCTGGACAGCGCCAGAACGTGCGCCACTGCCCTGGAAATGCCTGGAAGGATCTAGAAGGGGCGAGATAG

Protein sequence

MCGSSPQADTCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQILEEERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVRSTSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVETLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNEQGKKGKDLPIWGEVASKVHSAVGCDSCGRQRLARAPVPPRAPHRPDPSGQSWKLLEGSGQCQRPRPRPSSAWKDLGGAGQCQRPRESPSMAQKAPDASGQRQNVRHCPGNAWKDLEGAR

Homology

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BLAST of Lsi02G020800 vs. ExPASy Swiss-Prot
Match: Q8LBL5 (E3 ubiquitin-protein ligase PRT1 OS=Arabidopsis thaliana OX=3702 GN=PRT1 PE=2 SV=2)

HSP 1 Score: 228.0 bits (580), Expect = 1.7e-58
Identity = 124/286 (43.36%), Postives = 169/286 (59.09%), Query Frame = 0


Query: 10  TCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQIL 69
           +CGH+SCFWCVHKSMNGF ESHCP+CR PY HFP++C+ L+ L+ K+YP A+KKRE Q+L
Sbjct: 40  SCGHLSCFWCVHKSMNGFRESHCPICRDPYVHFPSVCQKLYFLLKKMYPLAHKKREEQVL 99

Query: 70  EEERKIGLFSPQFD-----SLACGSQAGI----KVEHLEDSANGKLNTITKNDDVVTELV 129
           +EE++   FSPQ D     S+  G    +    KVE   ++AN  L++ +   D+     
Sbjct: 100 KEEQERECFSPQIDLVLDLSVCSGDSLNVSDKQKVEECSNAAN-LLSSSSSRGDIP---C 159

Query: 130 LKENSEVVRSTSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFC 189
           + +N E    T  ++LN  ++   +     ++IS  D+LC  C +LL RPVVLNCGH +C
Sbjct: 160 IPKNQE---PTDAKALNVHENELLKDNKVSKQISKDDLLCSACKELLVRPVVLNCGHVYC 219

Query: 190 ESCI---NSQVETLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKS 249
           E C+     + E ++CQ C    PRGFP VCL L+Q L+E FPEEY+ R   VQ  LA +
Sbjct: 220 EGCVVDMAEESEKIKCQECNVCDPRGFPKVCLILEQLLEENFPEEYNSRSSKVQKTLAHN 279

Query: 250 IKHDIPT------SCSNEQGKKGKDLPIWGEVASKVHSAVGCDSCG 278
            K +I +      S SN+      D P      S VH   GCDSCG
Sbjct: 280 SKGNIQSYLKEGPSLSND---NNNDDPWLANPGSNVHFGAGCDSCG 315

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BLAST of Lsi02G020800 vs. ExPASy Swiss-Prot
Match: F4I443 (BRCA1-associated RING domain protein 1 OS=Arabidopsis thaliana OX=3702 GN=BARD1 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.9e-04
Identity = 45/172 (26.16%), Postives = 79/172 (45.93%), Query Frame = 0


Query: 134 ESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCI--NSQVETL 193
           E  N L +P     L+ +K+ + ++ C  C +LL RPV+L C H FC+SC+  +SQVE+ 
Sbjct: 3   EFTNMLMNPW---VLHLQKLEL-ELKCPLCLKLLNRPVLLPCDHVFCDSCVHKSSQVES- 62

Query: 194 ECQVCQFLQPRGFPNVCLELDQFLK--EQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNE 253
            C VC+   P+        ++  +   +      S+    +Q     + K+D   + ++ 
Sbjct: 63  GCPVCKSKHPKKARRDLRFMESVISIYKSLNAAVSVHLPQLQIPNDCNYKNDALNNSNSP 122

Query: 254 QGKKGKDLPIWGEVASKVHSAVGCDSCGRQRLARAPVPPRAPHRPDPSGQSW 302
           +  + +D  +  +  SK   + G DS  R     +P+P      P P  Q W
Sbjct: 123 KHGESEDSEMTDKDVSK--RSGGTDSSSRD---GSPLPTSEESDPRPKHQDW 164

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BLAST of Lsi02G020800 vs. ExPASy Swiss-Prot
Match: Q17RB8 (LON peptidase N-terminal domain and RING finger protein 1 OS=Homo sapiens OX=9606 GN=LONRF1 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 4.1e-04
Identity = 28/93 (30.11%), Postives = 48/93 (51.61%), Query Frame = 0


Query: 150 QEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVETLE-CQVC-----QFLQPRG 209
           +E I V+D  C  C +L F PV   CGH+FC++C+   ++    C +C     ++L  R 
Sbjct: 469 EELIDVSDFECSLCMRLFFEPVTTPCGHSFCKNCLERCLDHAPYCPLCKESLKEYLADRR 528

Query: 210 FPNVCLELDQFLKEQFPEEYSLRRDSVQFELAK 237
           +  V   L++ + +  P+E S R+     E A+
Sbjct: 529 Y-CVTQLLEELIVKYLPDELSERKKIYDEETAE 560

[Close]

BLAST of Lsi02G020800 vs. ExPASy Swiss-Prot
Match: D3YY23 (LON peptidase N-terminal domain and RING finger protein 1 OS=Mus musculus OX=10090 GN=Lonrf1 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 4.1e-04
Identity = 28/93 (30.11%), Postives = 48/93 (51.61%), Query Frame = 0


Query: 150 QEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVETLE-CQVC-----QFLQPRG 209
           +E I V+D  C  C +L F PV   CGH+FC++C+   ++    C +C     ++L  R 
Sbjct: 458 EELIDVSDFECSLCMRLFFEPVTTPCGHSFCKNCLERCLDHAPYCPLCKESLKEYLADRR 517

Query: 210 FPNVCLELDQFLKEQFPEEYSLRRDSVQFELAK 237
           +  V   L++ + +  P+E S R+     E A+
Sbjct: 518 Y-CVTQLLEELIVKYLPDELSERKKIYDEETAE 549

[Close]

BLAST of Lsi02G020800 vs. ExPASy TrEMBL
Match: A0A1S3C815 (E3 ubiquitin-protein ligase PRT1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497542 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 8.3e-133
Identity = 227/268 (84.70%), Postives = 245/268 (91.42%), Query Frame = 0


Query: 10  TCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQIL 69
           +CGHISCFWCVHKSMNGF ESHCP+CRRPYYHFPTICE+LHQLI+KIYP AYK+RE QIL
Sbjct: 110 SCGHISCFWCVHKSMNGFRESHCPICRRPYYHFPTICEILHQLIVKIYPTAYKRREAQIL 169

Query: 70  EEERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVR 129
           EEE+KIG FSPQFDSLACGSQAG+KVEHLEDSANG+LNT TKN D + EL+L+ENS+VV 
Sbjct: 170 EEEKKIGYFSPQFDSLACGSQAGMKVEHLEDSANGELNTNTKNGDAIAELILEENSDVVS 229

Query: 130 STSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVE 189
           STSV SLNSLQDPC QKT  QEKISVADVLC+ CTQLLFRPVV+NCGH FCESCINSQVE
Sbjct: 230 STSVVSLNSLQDPCAQKTQKQEKISVADVLCQACTQLLFRPVVINCGHVFCESCINSQVE 289

Query: 190 TLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNE 249
           TLECQVCQ LQPRGFPNVCLELDQFLKEQFPEEYS+RRDSVQ E A SIKHD PTSCS E
Sbjct: 290 TLECQVCQSLQPRGFPNVCLELDQFLKEQFPEEYSIRRDSVQLEQANSIKHDNPTSCSIE 349

Query: 250 QGKKGKDLPIWGEVASKVHSAVGCDSCG 278
           +GKKG+ LP WG+VASKVHSAVGCDSCG
Sbjct: 350 EGKKGQYLPRWGDVASKVHSAVGCDSCG 377

[Close]

BLAST of Lsi02G020800 vs. ExPASy TrEMBL
Match: A0A0A0K4I8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G339130 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 2.3e-130
Identity = 222/267 (83.15%), Postives = 244/267 (91.39%), Query Frame = 0


Query: 11  CGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQILE 70
           CGHISCFWCVHK MNGF ESHCP+CRR YYHFPTICE+LHQLILKIYPA+YK+RE+QILE
Sbjct: 32  CGHISCFWCVHKCMNGFRESHCPICRRSYYHFPTICEILHQLILKIYPASYKRRESQILE 91

Query: 71  EERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVRS 130
            E+KIG FSPQFDSLACGSQAG+KVEHLEDSANG+LNT TKNDD V EL+L+ENS+VV S
Sbjct: 92  VEKKIGFFSPQFDSLACGSQAGMKVEHLEDSANGELNTNTKNDDAVAELILEENSDVVSS 151

Query: 131 TSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVET 190
           TSV SLNSLQDPC QKT NQEKISVADVLC+ CTQLLFRPVV+NCGH FCESCINSQVET
Sbjct: 152 TSVVSLNSLQDPCAQKTQNQEKISVADVLCQACTQLLFRPVVMNCGHVFCESCINSQVET 211

Query: 191 LECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNEQ 250
           LECQVCQ LQPRGF NVCLELDQFLKE+FPEEYS+RRDSVQ +LA S+KHD PTSCSNE+
Sbjct: 212 LECQVCQSLQPRGFRNVCLELDQFLKEKFPEEYSIRRDSVQLKLANSMKHDNPTSCSNEE 271

Query: 251 GKKGKDLPIWGEVASKVHSAVGCDSCG 278
           GKKG+ LP WG+VASKVH+ +GCD CG
Sbjct: 272 GKKGEYLPRWGDVASKVHTFIGCDYCG 298

[Close]

BLAST of Lsi02G020800 vs. ExPASy TrEMBL
Match: A0A6J1KS10 (E3 ubiquitin-protein ligase PRT1 OS=Cucurbita maxima OX=3661 GN=LOC111498135 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.7e-123
Identity = 213/268 (79.48%), Postives = 236/268 (88.06%), Query Frame = 0


Query: 10  TCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQIL 69
           +CGHISCFWCVHKSMNGF ESHCP+CRRPYYHFPTICEMLH LILK+YP AYK+R+NQ L
Sbjct: 68  SCGHISCFWCVHKSMNGFRESHCPICRRPYYHFPTICEMLHLLILKMYPMAYKRRQNQTL 127

Query: 70  EEERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVR 129
           EEERK+GLFSPQ+DSLACGS+AG K+EHLEDSANG++N  TKND+VV EL+L+ENSEVVR
Sbjct: 128 EEERKLGLFSPQYDSLACGSRAGEKMEHLEDSANGEMNANTKNDNVVAELILEENSEVVR 187

Query: 130 STSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVE 189
           S SVE LNSL D   QKT NQE ++VADVLCR CTQLL RPVVLNCGHAFCESCIN +VE
Sbjct: 188 SASVEILNSLGDLHGQKTQNQETVTVADVLCRACTQLLLRPVVLNCGHAFCESCINLRVE 247

Query: 190 TLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNE 249
           TLECQVCQ LQPRGFPNVCLELDQFLKEQ PEEY LR DSVQ +LA++ KH+ PTSCS+E
Sbjct: 248 TLECQVCQSLQPRGFPNVCLELDQFLKEQLPEEYKLRSDSVQLKLAETFKHESPTSCSSE 307

Query: 250 QGKKGKDLPIWGEVASKVHSAVGCDSCG 278
            G KG+ LP W EVASKVH AVGCDSCG
Sbjct: 308 GGMKGQGLPRWDEVASKVHPAVGCDSCG 335

[Close]

BLAST of Lsi02G020800 vs. ExPASy TrEMBL
Match: A0A6J1HAU1 (E3 ubiquitin-protein ligase PRT1 OS=Cucurbita moschata OX=3662 GN=LOC111461119 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 1.0e-122
Identity = 213/269 (79.18%), Postives = 237/269 (88.10%), Query Frame = 0


Query: 10  TCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQIL 69
           +CGHISCFWCVHKSMNGF ESHCP+CRRPYYHFPTICEMLH LILK+YP AYK+R+NQ L
Sbjct: 68  SCGHISCFWCVHKSMNGFRESHCPICRRPYYHFPTICEMLHLLILKMYPMAYKRRQNQTL 127

Query: 70  EEERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVR 129
           EEERK+GLFSPQ+DSLACGS+AG K+EHLEDSANG++N  TKND+VV EL+L+ENSEVVR
Sbjct: 128 EEERKLGLFSPQYDSLACGSRAGEKIEHLEDSANGEMNANTKNDNVVAELILEENSEVVR 187

Query: 130 STSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVE 189
           S SVE LNSL D   Q T NQE ++VADVLCR CTQLL RPVVLNCGHAFCESCIN +VE
Sbjct: 188 SASVEILNSLGDLHGQNTQNQETVTVADVLCRACTQLLLRPVVLNCGHAFCESCINLRVE 247

Query: 190 TLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNE 249
           TLECQVCQ LQPRGFPNVCLELDQFLKEQ PEEY LR DSVQ +LA++ KH+ PTSCS+E
Sbjct: 248 TLECQVCQSLQPRGFPNVCLELDQFLKEQLPEEYKLRSDSVQLKLAETFKHESPTSCSSE 307

Query: 250 Q-GKKGKDLPIWGEVASKVHSAVGCDSCG 278
           + GKKG+ LP W EVASKVH AVGCDSCG
Sbjct: 308 EGGKKGQGLPRWDEVASKVHPAVGCDSCG 336

[Close]

BLAST of Lsi02G020800 vs. ExPASy TrEMBL
Match: A0A1S3C6B5 (E3 ubiquitin-protein ligase PRT1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497542 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 9.3e-116
Identity = 201/236 (85.17%), Postives = 216/236 (91.53%), Query Frame = 0


Query: 10  TCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQIL 69
           +CGHISCFWCVHKSMNGF ESHCP+CRRPYYHFPTICE+LHQLI+KIYP AYK+RE QIL
Sbjct: 110 SCGHISCFWCVHKSMNGFRESHCPICRRPYYHFPTICEILHQLIVKIYPTAYKRREAQIL 169

Query: 70  EEERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVR 129
           EEE+KIG FSPQFDSLACGSQAG+KVEHLEDSANG+LNT TKN D + EL+L+ENS+VV 
Sbjct: 170 EEEKKIGYFSPQFDSLACGSQAGMKVEHLEDSANGELNTNTKNGDAIAELILEENSDVVS 229

Query: 130 STSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVE 189
           STSV SLNSLQDPC QKT  QEKISVADVLC+ CTQLLFRPVV+NCGH FCESCINSQVE
Sbjct: 230 STSVVSLNSLQDPCAQKTQKQEKISVADVLCQACTQLLFRPVVINCGHVFCESCINSQVE 289

Query: 190 TLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTS 246
           TLECQVCQ LQPRGFPNVCLELDQFLKEQFPEEYS+RRDSVQ E A SIKHD PTS
Sbjct: 290 TLECQVCQSLQPRGFPNVCLELDQFLKEQFPEEYSIRRDSVQLEQANSIKHDNPTS 345

[Close]

BLAST of Lsi02G020800 vs. NCBI nr
Match: XP_038877741.1 (E3 ubiquitin-protein ligase PRT1 isoform X1 [Benincasa hispida])

HSP 1 Score: 511.1 bits (1315), Expect = 7.7e-141
Identity = 241/268 (89.93%), Postives = 252/268 (94.03%), Query Frame = 0


Query: 10  TCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQIL 69
           +CGHISCFWCVHKSMNGF ESHCP+CR PYYHFPTICEMLHQLILKIYPA YKKRENQIL
Sbjct: 68  SCGHISCFWCVHKSMNGFRESHCPICRHPYYHFPTICEMLHQLILKIYPAVYKKRENQIL 127

Query: 70  EEERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVR 129
           EEERKIG FSPQFDSLACGSQAGIKVEHLEDSANG+LNTITKNDDVVTEL+L+E SEVV 
Sbjct: 128 EEERKIGFFSPQFDSLACGSQAGIKVEHLEDSANGELNTITKNDDVVTELILEETSEVVS 187

Query: 130 STSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVE 189
           S+SV+SLNSLQDPC QKT NQEKISVADVLC+ CTQLLFRPVVLNCGHAFCESCINSQVE
Sbjct: 188 SSSVKSLNSLQDPCAQKTQNQEKISVADVLCQACTQLLFRPVVLNCGHAFCESCINSQVE 247

Query: 190 TLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNE 249
           TLECQVCQF+QPRGFPNVCLELDQFLKEQFPEEYSLRRDS+Q  LAKSIKHD P SCSNE
Sbjct: 248 TLECQVCQFVQPRGFPNVCLELDQFLKEQFPEEYSLRRDSIQLVLAKSIKHDSPISCSNE 307

Query: 250 QGKKGKDLPIWGEVASKVHSAVGCDSCG 278
           +GKKGKDLP WGEVASKVH  VGCDSCG
Sbjct: 308 EGKKGKDLPRWGEVASKVHYGVGCDSCG 335

[Close]

BLAST of Lsi02G020800 vs. NCBI nr
Match: XP_008457982.1 (PREDICTED: E3 ubiquitin-protein ligase PRT1 isoform X1 [Cucumis melo])

HSP 1 Score: 483.4 bits (1243), Expect = 1.7e-132
Identity = 227/268 (84.70%), Postives = 245/268 (91.42%), Query Frame = 0


Query: 10  TCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQIL 69
           +CGHISCFWCVHKSMNGF ESHCP+CRRPYYHFPTICE+LHQLI+KIYP AYK+RE QIL
Sbjct: 110 SCGHISCFWCVHKSMNGFRESHCPICRRPYYHFPTICEILHQLIVKIYPTAYKRREAQIL 169

Query: 70  EEERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVR 129
           EEE+KIG FSPQFDSLACGSQAG+KVEHLEDSANG+LNT TKN D + EL+L+ENS+VV 
Sbjct: 170 EEEKKIGYFSPQFDSLACGSQAGMKVEHLEDSANGELNTNTKNGDAIAELILEENSDVVS 229

Query: 130 STSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVE 189
           STSV SLNSLQDPC QKT  QEKISVADVLC+ CTQLLFRPVV+NCGH FCESCINSQVE
Sbjct: 230 STSVVSLNSLQDPCAQKTQKQEKISVADVLCQACTQLLFRPVVINCGHVFCESCINSQVE 289

Query: 190 TLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNE 249
           TLECQVCQ LQPRGFPNVCLELDQFLKEQFPEEYS+RRDSVQ E A SIKHD PTSCS E
Sbjct: 290 TLECQVCQSLQPRGFPNVCLELDQFLKEQFPEEYSIRRDSVQLEQANSIKHDNPTSCSIE 349

Query: 250 QGKKGKDLPIWGEVASKVHSAVGCDSCG 278
           +GKKG+ LP WG+VASKVHSAVGCDSCG
Sbjct: 350 EGKKGQYLPRWGDVASKVHSAVGCDSCG 377

[Close]

BLAST of Lsi02G020800 vs. NCBI nr
Match: XP_004139635.1 (E3 ubiquitin-protein ligase PRT1 [Cucumis sativus] >KGN44590.1 hypothetical protein Csa_015910 [Cucumis sativus])

HSP 1 Score: 475.3 bits (1222), Expect = 4.7e-130
Identity = 222/267 (83.15%), Postives = 244/267 (91.39%), Query Frame = 0


Query: 11  CGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQILE 70
           CGHISCFWCVHK MNGF ESHCP+CRR YYHFPTICE+LHQLILKIYPA+YK+RE+QILE
Sbjct: 32  CGHISCFWCVHKCMNGFRESHCPICRRSYYHFPTICEILHQLILKIYPASYKRRESQILE 91

Query: 71  EERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVRS 130
            E+KIG FSPQFDSLACGSQAG+KVEHLEDSANG+LNT TKNDD V EL+L+ENS+VV S
Sbjct: 92  VEKKIGFFSPQFDSLACGSQAGMKVEHLEDSANGELNTNTKNDDAVAELILEENSDVVSS 151

Query: 131 TSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVET 190
           TSV SLNSLQDPC QKT NQEKISVADVLC+ CTQLLFRPVV+NCGH FCESCINSQVET
Sbjct: 152 TSVVSLNSLQDPCAQKTQNQEKISVADVLCQACTQLLFRPVVMNCGHVFCESCINSQVET 211

Query: 191 LECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNEQ 250
           LECQVCQ LQPRGF NVCLELDQFLKE+FPEEYS+RRDSVQ +LA S+KHD PTSCSNE+
Sbjct: 212 LECQVCQSLQPRGFRNVCLELDQFLKEKFPEEYSIRRDSVQLKLANSMKHDNPTSCSNEE 271

Query: 251 GKKGKDLPIWGEVASKVHSAVGCDSCG 278
           GKKG+ LP WG+VASKVH+ +GCD CG
Sbjct: 272 GKKGEYLPRWGDVASKVHTFIGCDYCG 298

[Close]

BLAST of Lsi02G020800 vs. NCBI nr
Match: XP_038877742.1 (E3 ubiquitin-protein ligase PRT1 isoform X2 [Benincasa hispida])

HSP 1 Score: 475.3 bits (1222), Expect = 4.7e-130
Identity = 228/254 (89.76%), Postives = 238/254 (93.70%), Query Frame = 0


Query: 24  MNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQILEEERKIGLFSPQFD 83
           MNGF ESHCP+CR PYYHF TICEMLHQLILKIYPAAYKKRENQILEEERKIG FSPQFD
Sbjct: 1   MNGFCESHCPICRHPYYHFSTICEMLHQLILKIYPAAYKKRENQILEEERKIGFFSPQFD 60

Query: 84  SLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVRSTSVESLNSLQDPC 143
           SLACGSQAGIKVEHLEDSANG+LNTITKNDDVVTEL+L+E SEVV S+SV+SLNSLQDPC
Sbjct: 61  SLACGSQAGIKVEHLEDSANGELNTITKNDDVVTELILEETSEVVSSSSVKSLNSLQDPC 120

Query: 144 TQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVETLECQVCQFLQPRG 203
            QKT NQEKISVADVLC+ CTQLLFRPVVLNCGHAFCESCINSQVETLECQVCQF+QPRG
Sbjct: 121 AQKTQNQEKISVADVLCQACTQLLFRPVVLNCGHAFCESCINSQVETLECQVCQFVQPRG 180

Query: 204 FPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNEQGKKGKDLPIWGEV 263
           FPNVCLELDQFLKEQFPEEYSLRRDS+Q  LAKSIKHD P SCSNE+GKKGKDLP WGEV
Sbjct: 181 FPNVCLELDQFLKEQFPEEYSLRRDSIQLVLAKSIKHDSPISCSNEEGKKGKDLPRWGEV 240

Query: 264 ASKVHSAVGCDSCG 278
           ASKVH  VGCDSCG
Sbjct: 241 ASKVHYGVGCDSCG 254

[Close]

BLAST of Lsi02G020800 vs. NCBI nr
Match: XP_023515020.1 (E3 ubiquitin-protein ligase PRT1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.0 bits (1164), Expect = 2.5e-123
Identity = 213/268 (79.48%), Postives = 236/268 (88.06%), Query Frame = 0


Query: 10  TCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQIL 69
           +CGHISCFWCVHKSMNGF ESHCP+CRRPYYHFPTICEMLH LILK+YP AYK+R+NQ L
Sbjct: 68  SCGHISCFWCVHKSMNGFRESHCPICRRPYYHFPTICEMLHLLILKMYPMAYKRRQNQTL 127

Query: 70  EEERKIGLFSPQFDSLACGSQAGIKVEHLEDSANGKLNTITKNDDVVTELVLKENSEVVR 129
           EEERK+GLFSPQ+DSLACGS+AG K+EHLEDSANG++N  TKND+VV EL+L+ENSEVVR
Sbjct: 128 EEERKLGLFSPQYDSLACGSRAGEKIEHLEDSANGEMNANTKNDNVVAELILEENSEVVR 187

Query: 130 STSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCINSQVE 189
           S SVE LNSL D   Q T NQE ++VADVLCR CTQLL RPVVLNCGHAFCESCIN +VE
Sbjct: 188 SASVEILNSLGDLHGQNTQNQETVTVADVLCRACTQLLLRPVVLNCGHAFCESCINLRVE 247

Query: 190 TLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNE 249
           TLECQVCQ LQPRGFPNVCLELDQFLKEQ PEEY LR DSVQ +LA++ KH+ PTSCS+E
Sbjct: 248 TLECQVCQSLQPRGFPNVCLELDQFLKEQLPEEYKLRSDSVQLKLAETFKHESPTSCSSE 307

Query: 250 QGKKGKDLPIWGEVASKVHSAVGCDSCG 278
            GKKG+ LP W EVASKVH AVGCDSCG
Sbjct: 308 GGKKGQGLPRWDEVASKVHPAVGCDSCG 335

[Close]

BLAST of Lsi02G020800 vs. TAIR 10
Match: AT3G24800.1 (proteolysis 1 )

HSP 1 Score: 228.0 bits (580), Expect = 1.2e-59
Identity = 124/286 (43.36%), Postives = 169/286 (59.09%), Query Frame = 0


Query: 10  TCGHISCFWCVHKSMNGFHESHCPMCRRPYYHFPTICEMLHQLILKIYPAAYKKRENQIL 69
           +CGH+SCFWCVHKSMNGF ESHCP+CR PY HFP++C+ L+ L+ K+YP A+KKRE Q+L
Sbjct: 40  SCGHLSCFWCVHKSMNGFRESHCPICRDPYVHFPSVCQKLYFLLKKMYPLAHKKREEQVL 99

Query: 70  EEERKIGLFSPQFD-----SLACGSQAGI----KVEHLEDSANGKLNTITKNDDVVTELV 129
           +EE++   FSPQ D     S+  G    +    KVE   ++AN  L++ +   D+     
Sbjct: 100 KEEQERECFSPQIDLVLDLSVCSGDSLNVSDKQKVEECSNAAN-LLSSSSSRGDIP---C 159

Query: 130 LKENSEVVRSTSVESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFC 189
           + +N E    T  ++LN  ++   +     ++IS  D+LC  C +LL RPVVLNCGH +C
Sbjct: 160 IPKNQE---PTDAKALNVHENELLKDNKVSKQISKDDLLCSACKELLVRPVVLNCGHVYC 219

Query: 190 ESCI---NSQVETLECQVCQFLQPRGFPNVCLELDQFLKEQFPEEYSLRRDSVQFELAKS 249
           E C+     + E ++CQ C    PRGFP VCL L+Q L+E FPEEY+ R   VQ  LA +
Sbjct: 220 EGCVVDMAEESEKIKCQECNVCDPRGFPKVCLILEQLLEENFPEEYNSRSSKVQKTLAHN 279

Query: 250 IKHDIPT------SCSNEQGKKGKDLPIWGEVASKVHSAVGCDSCG 278
            K +I +      S SN+      D P      S VH   GCDSCG
Sbjct: 280 SKGNIQSYLKEGPSLSND---NNNDDPWLANPGSNVHFGAGCDSCG 315

[Close]

BLAST of Lsi02G020800 vs. TAIR 10
Match: AT1G04020.2 (breast cancer associated RING 1 )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 45/172 (26.16%), Postives = 79/172 (45.93%), Query Frame = 0


Query: 134 ESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCI--NSQVETL 193
           E  N L +P     L+ +K+ + ++ C  C +LL RPV+L C H FC+SC+  +SQVE+ 
Sbjct: 3   EFTNMLMNPW---VLHLQKLEL-ELKCPLCLKLLNRPVLLPCDHVFCDSCVHKSSQVES- 62

Query: 194 ECQVCQFLQPRGFPNVCLELDQFLK--EQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNE 253
            C VC+   P+        ++  +   +      S+    +Q     + K+D   + ++ 
Sbjct: 63  GCPVCKSKHPKKGKRDLRFMESVISIYKSLNAAVSVHLPQLQIPNDCNYKNDALNNSNSP 122

Query: 254 QGKKGKDLPIWGEVASKVHSAVGCDSCGRQRLARAPVPPRAPHRPDPSGQSW 302
           +  + +D  +  +  SK   + G DS  R     +P+P      P P  Q W
Sbjct: 123 KHGESEDSEMTDKDVSK--RSGGTDSSSRD---GSPLPTSEESDPRPKHQDW 164

[Close]

BLAST of Lsi02G020800 vs. TAIR 10
Match: AT1G04020.1 (breast cancer associated RING 1 )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 45/172 (26.16%), Postives = 79/172 (45.93%), Query Frame = 0


Query: 134 ESLNSLQDPCTQKTLNQEKISVADVLCRGCTQLLFRPVVLNCGHAFCESCI--NSQVETL 193
           E  N L +P     L+ +K+ + ++ C  C +LL RPV+L C H FC+SC+  +SQVE+ 
Sbjct: 3   EFTNMLMNPW---VLHLQKLEL-ELKCPLCLKLLNRPVLLPCDHVFCDSCVHKSSQVES- 62

Query: 194 ECQVCQFLQPRGFPNVCLELDQFLK--EQFPEEYSLRRDSVQFELAKSIKHDIPTSCSNE 253
            C VC+   P+        ++  +   +      S+    +Q     + K+D   + ++ 
Sbjct: 63  GCPVCKSKHPKKARRDLRFMESVISIYKSLNAAVSVHLPQLQIPNDCNYKNDALNNSNSP 122

Query: 254 QGKKGKDLPIWGEVASKVHSAVGCDSCGRQRLARAPVPPRAPHRPDPSGQSW 302
           +  + +D  +  +  SK   + G DS  R     +P+P      P P  Q W
Sbjct: 123 KHGESEDSEMTDKDVSK--RSGGTDSSSRD---GSPLPTSEESDPRPKHQDW 164

The following BLAST results are available for this feature:

BLAST of Lsi02G020800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LBL5	1.7e-58	43.36	E3 ubiquitin-protein ligase PRT1 OS=Arabidopsis thaliana OX=3702 GN=PRT1 PE=2 SV...	[more]
F4I443	1.9e-04	26.16	BRCA1-associated RING domain protein 1 OS=Arabidopsis thaliana OX=3702 GN=BARD1 ...	[more]
Q17RB8	4.1e-04	30.11	LON peptidase N-terminal domain and RING finger protein 1 OS=Homo sapiens OX=960...	[more]
D3YY23	4.1e-04	30.11	LON peptidase N-terminal domain and RING finger protein 1 OS=Mus musculus OX=100...	[more]

BLAST of Lsi02G020800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C815	8.3e-133	84.70	E3 ubiquitin-protein ligase PRT1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497...	[more]
A0A0A0K4I8	2.3e-130	83.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G339130 PE=4 SV=1	[more]
A0A6J1KS10	2.7e-123	79.48	E3 ubiquitin-protein ligase PRT1 OS=Cucurbita maxima OX=3661 GN=LOC111498135 PE=...	[more]
A0A6J1HAU1	1.0e-122	79.18	E3 ubiquitin-protein ligase PRT1 OS=Cucurbita moschata OX=3662 GN=LOC111461119 P...	[more]
A0A1S3C6B5	9.3e-116	85.17	E3 ubiquitin-protein ligase PRT1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497...	[more]

BLAST of Lsi02G020800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877741.1	7.7e-141	89.93	E3 ubiquitin-protein ligase PRT1 isoform X1 [Benincasa hispida]	[more]
XP_008457982.1	1.7e-132	84.70	PREDICTED: E3 ubiquitin-protein ligase PRT1 isoform X1 [Cucumis melo]	[more]
XP_004139635.1	4.7e-130	83.15	E3 ubiquitin-protein ligase PRT1 [Cucumis sativus] >KGN44590.1 hypothetical prot...	[more]
XP_038877742.1	4.7e-130	89.76	E3 ubiquitin-protein ligase PRT1 isoform X2 [Benincasa hispida]	[more]
XP_023515020.1	2.5e-123	79.48	E3 ubiquitin-protein ligase PRT1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi02G020800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24800.1	1.2e-59	43.36	proteolysis 1	[more]
AT1G04020.2	1.3e-05	26.16	breast cancer associated RING 1	[more]
AT1G04020.1	1.3e-05	26.16	breast cancer associated RING 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 2..35 e-value: 1.9 score: 9.0 coord: 160..196 e-value: 4.7E-5 score: 32.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 160..197 score: 10.305834
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 108..226 e-value: 3.6E-10 score: 41.5
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 4..78 e-value: 1.2E-6 score: 30.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 284..368
None	No IPR available	PANTHER	PTHR15898:SF15	E3 UBIQUITIN-PROTEIN LIGASE PRT1-LIKE	coord: 107..278
None	No IPR available	PANTHER	PTHR15898:SF15	E3 UBIQUITIN-PROTEIN LIGASE PRT1-LIKE	coord: 10..103
None	No IPR available	PANTHER	PTHR15898	UNCHARACTERIZED	coord: 107..278 coord: 10..103
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 151..200
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 175..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G020800.1	Lsi02G020800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding