Lsi02G020250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G020250
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionLipase_3 domain-containing protein
Locationchr02: 26272122 .. 26277188 (-)
RNA-Seq ExpressionLsi02G020250
SyntenyLsi02G020250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAACATCATTTTTCTTCTCTGTCAACAACATTCATGTACATTCGACACGAACCCACCAAATAAATTAATATACAAATATATAAAACTCTGAATACTGCCTGTGAAAACTGTGATCCCCAGAATCAGAATTTTCTCTTCATTAAGTTTCTGTTCCTATTCACCGCCAACTCAAATCCTTGCTCTCTGGGCTGCATTTTCATTGTGTTAGAAATATAGTGGAACCATCAGATTGTCGAGAAATCGAGGTATGATATATGTTCTACATTCCAATCTATTGTTCGTTTCTTTTTTCGCAAATTTTTTAGTTCGCATTTATGTGAAGTTTGTTTGATCGATTTATATGGGGTTGCGTTACTTTGCTATTATCGGTTTGAATTGTCTAAATTGGAGTTTGTTAGGCTGGAAAACCTCTGTTTTCAACAATGTCGGCCTTTTTACTTGTTGGAAATTAATTTAAGATCGATAGTTTCGAGGGAAGTAGTTGAGGTGATTGCTAGTTGATTCTGATTTGAAACCTTTTCATTGTTTCATCACACCCTCTCCTCTCCTTCCTTTGCTTTACGTGAATTGGTTTCGACATTCTCCTGTATTTCGCTTGAGTTACTTTCACTCTCGAGAGTGCAAATACACGAGTCGTTTCAAAATTTGTTCGTCGCCCACATTTGTCTCGTGGGCATGTATCTTTGTTAGTATAGAGTAGTATCTATTCAATTTTTGCAGTTTCAGGATCCCCTCTACTTCACTTGAGCTACTTCGGATTTCGAGACTGAAAACTGTTCTCGCCCGTCATTCCAATGTGGTTAGCCCCCGCTTGCGTAATTAACGCACAACTTTGTCTTATGGACTACGGAAAAGAAAGATGCACTTTGTTAGTATAGATATTAGACAATATATATACATTCGTTCAAGTCTTTTCTCACCATGCTTTCATAGTCTCATGGTCCTCTCTACTTGTACTTGACTTAAACGTCGAGAGTAAAATGGGAGTTCCAACATTCTTTGCTTCTACTCGCATAATTTAATGTGTCGTTGCGTTTCGTCTTATGGACCCGTGAAATCTATATATTCTTTCAACTCTTTCAAATCAAAGTATGTTGCTAAGATCAGATGCTTTCTTGCATAGTGTACTGTGTAACAATGGGAATATCCAAAACAGTCCGGTCTTTTTTGGTAGTAAAAAATTTGAAGTATTCTATGTTGATTTTACATATGTTATCTTTGTAAACGGATGAATTTAAACATAGGTGTTAGCAACAGGATTTGGAGTTTAGCTGTCTTTGTTATTGGTGTGAAGCCCACTTATCCTTTTCTACTTAAACATATTAATTTAATCACCTCTCTCTTGTACATAAATTCAACAATTACTTGTATAGCTTATTGGGTTCAATTTATTTTCGTTACTAACAAGAAGATCACTGCTCCACTGAAATGGGATATCATCAGTTCCATTTCTAAGGACCAAACGGACAAACCAAAGATCTGATCGATTATTAAATTTTTCTGATTGATTATTAAATTCTTTCTCATATCTATATAACCTCTTCATATAAGATCAATACAGTTTGAGATTGTTTAAATTTTTCAGCTTCCTTGAAATTTACTCTCTCTCTTTCTCTCTCGTATTGACATGGTTTCTTCTTTGTCTTCCTTTTTCAGGAATCCCTTGTTGTGAAGAGGGTCTAATAAACACAAATAGTTTCTTTAGTCCATCAACTGACAGTTCTTCAAGAGTCATCATGGCTTGTACTTCAGTGGCTATTGGCAATTCTCCAGCCGCTGCAATGACAAAAGACATCATCTTCAAAGAATATAATGGGCTTCGCCGTTCGCATTCGAGCAAGGACGTTAGAGAACGAGCTTGCATCAAAAGATCTTATTCTGATAACCAGATTTGTTACTCTGTAAACAAAATTCATGCTACTTCAACACAGCCAAAGCCAAAAAACAACAATAACAGCTCTATGGGAATGGGGATTTTTCCTTTGAAATTTTCAGGGTCTTTTCTTCCTAATGCGGTCAGATCATTTTTGTTTGACATGGAGGAGACCTCAAAGGATCTCAGTAGTGAAGAGGAGGTGACAAAGAGAGCAAACTGGATCGAGAGATTGTTGGAGATTCGAAGTCGTTGGCGGAAAAAGCAGGAAAAAGGAGTTGAGAATGATCTCTATGCAGATCATGATGAATCTGCCGAGAGTTTATGTGGTGGAGATGATGGTGGCTGTGAAGTGGATTATTATGACTCTGAAGATGAAGAAGGGCTTACTTTTGATACTGAATCATTTTCAAGATTTCTAATCCGGGTTCCATTGTCTGATACCAAGGTGTTTTCTCAGCTGGCTTTCCTGTCAAACATGGCATACGTTATACAAAAAATAAAGGTACTAGTCCGGCCATTTGCACTTTTTTTTTTTTTTTGCTACAAAGACTTACTTTTATGTTGGAAGGAACTTTAAAGTCGATAAGCTTATTCGTTTGTATGCTTGAAAAGACCAAGTTTTCTATGCATTTCATTTCCAAAATTTATTATTATTCTTGTATATTACTATTTATGTCTATTTATAATGCATAACAAAGTGTTTGATATGTGCCTAACAAGTGTCATACTTATTCAGTTTATACGATTAGTGTTTGAATCGTTTATTGTGTGTTCAACAAGTATTCAAATATCGGAGTGTCAGTCATATGTTGGGCATGGACACGTTATTCAAACTAAAACTAAAGTGGCTGTGCTTTGTAGCTTATTCTTAGATCAATGTCAAATGTGTATTTTAGTGTCCATTGAATTTCATTATTTTAATTACAGGCAGAGGATTTAGAAATATACCATGGCCTGCAATTTGTAACATCCTCCTTGCGCAAGAAAGCAGATGCTGCAGCCATTAATATCAAAGAAAAACTGGATCAGCACTCAACTTGTATTTCTGATGAGGCCTTCAATGCTGGTGAATCTTATTCAGTGGAAGCATTGGAGCTGGAGAGGACACGACAAATCCCTACAGCTGTTGCTTATGAGATTGCTGCGACAGCTGCATCTTATGTTCACTCGAGAGCCAAGAATCCTTCATCCCATCCTTTGGAATCACAAGAGAAGGGAGAGAGTTCGACTCGGGCATATAATCCCGAGGTGGCTGCCTATGTTGCAGCTTCAACAATGACGGCTGTGGTTGCTGCTGAGGAGGTGCAGAAACAGGAGACTGCAAAAGACCTCCAATCTCTTCACTCTTCTCCTTGTGAATGGTTTGTTTGTGACGATACTCACACCTTGACCCGATGCTTCATTATTCAGGTGCTTCCTCTAATCTATTCCCTTTGAGAGTTGTTTTTAATAGATTCATGAACTTTTAAGATTGGGTCTATCCAGTCTCTATAATTTCAGAAGTTTTTAATAAGTTTTTCAACTTTCAATTTCATGTCTAATAAGTCTTTGTTACTAACTTCGTCAATTTAACATTTGAATATGTCAGTTTTATTATCTGAGGATGATATGATAAGTTGATTTTAGGTAGGCTCAAGCGGGTTGATGGCATGAATGTTAAACTAACAAAGTTAATGATAAAGATTTATTAGATACAATATTGACAGTTTAGGGGCCCACTAGAATCTTTTTAATCTATAGGAACTAAATAGATACAAGCTTGAAAGTTTGTAAACCAATAGAAACTTTTTAAACTATAGTGGGAATAATAACTCCAACTAAAAAAGTTTAGAAACCAAACTTATAATTTAACCTATATTAGTTTGTCTGAAATACAGGGATCGGATTCCTTGGCATCTTGGCAAGCAAATCTTTTTTTTGAGCCCACCAAATTTGAGGTAAAGCATGGCCTCTATTATTTACTAAGTCAGAAGTGAAAACTACATGAAGTTTTGTAATTAAAAATGAACTTAATATAGGGGACAGATGTGCTTGTCCATAGAGGAATATATGAAGCTGCAAAGGGAATATACAAGCAATTCATGCCAGAAATTATAGACCATTTGAAAAAATATGGAACCCATGCAAAGTTTCAGTTTACTGGCCATTCTCTTGGAGGCAGTCTTTCCCTTTTGGTGCATTTGATGCTACTAAAGAATGGGATTGTCAAACCTACTATGCTTCAACCAGTTGTCACTTTTGGCTCTCCATTTGTGTTCTGTGGTGGACACAAGATTCTAAATGAATTGGGTTTGGATGAAGACGATATCTATTGCATCATAATGCATCGAGATATCGTTCCAAGGGCCTTCTCCTGCAACTATCCCAACCATGTCGCTGCTGTACTCAAGCGGTTGAGTGGATCGTTTCGGTCTCATTCTTGTCTCAACAAAAGTGTAAGCAGTCTTAGGATCTGCTTTCTATTCGACTCTTATAGTTTTAAGTTAAATTATACAGAATGTGTAGCTGGTTTCAAATTTCAAATTTACTCTACTTATATTTGTGTTTATGCAGAAATTACTATACACTCCATTGGGTAAGCTATTCATTTTGCAACCTGATGAGATGGCATCTCCCCCTCATCCTATGCTCCCTCTGGGAAGTGCCCTCTATACTTTGGATAGCACCCAAAATGGATACTCCAAAGGCCTCCTTAGGGCTTTTCTAAATTGCCCTCATCCCCTTGAAACACTCAGTGACCCTACAGCTTATGGGTCAGAGGGCACCATTTTAAGAGACCATGACTCAAGTTTCTATCTCAAGGCTTTGAATGGGGTTCTTAAACAACACACAAAGATGAATGTTGAGAAAGTCAGAAAGCAGAGGAAATTGTTATGGCCATTACTTGCTTCACCATCTCCAAAGTTGTGGAGCCATGATGGAAATTTGGACAATAATAATAGTTTGCTGTCCAATGAGATAATGACAGGTGTTTAAGACCATTTTCCATGAGCTCTGCCTTGCTTGTGTAGATGGATGGCTCACTTTTCAGCTTGGTTATCATCTTGTAAATCTCTTTTGTAAATGCATACAATAATATATCTTTCCAGAAAGGGAGAAAAAGGTATTTATTCTTATAGATCTTTTGTAAAATAGTTATACAAAAAAGATAGAGTAATTGGAGAGGAAAAAAAATATTGTTGATGAGCC

mRNA sequence

CAAAACATCATTTTTCTTCTCTGTCAACAACATTCATGTACATTCGACACGAACCCACCAAATAAATTAATATACAAATATATAAAACTCTGAATACTGCCTGTGAAAACTGTGATCCCCAGAATCAGAATTTTCTCTTCATTAAGTTTCTGTTCCTATTCACCGCCAACTCAAATCCTTGCTCTCTGGGCTGCATTTTCATTGTGTTAGAAATATAGTGGAACCATCAGATTGTCGAGAAATCGAGGAATCCCTTGTTGTGAAGAGGGTCTAATAAACACAAATAGTTTCTTTAGTCCATCAACTGACAGTTCTTCAAGAGTCATCATGGCTTGTACTTCAGTGGCTATTGGCAATTCTCCAGCCGCTGCAATGACAAAAGACATCATCTTCAAAGAATATAATGGGCTTCGCCGTTCGCATTCGAGCAAGGACGTTAGAGAACGAGCTTGCATCAAAAGATCTTATTCTGATAACCAGATTTGTTACTCTGTAAACAAAATTCATGCTACTTCAACACAGCCAAAGCCAAAAAACAACAATAACAGCTCTATGGGAATGGGGATTTTTCCTTTGAAATTTTCAGGGTCTTTTCTTCCTAATGCGGTCAGATCATTTTTGTTTGACATGGAGGAGACCTCAAAGGATCTCAGTAGTGAAGAGGAGGTGACAAAGAGAGCAAACTGGATCGAGAGATTGTTGGAGATTCGAAGTCGTTGGCGGAAAAAGCAGGAAAAAGGAGTTGAGAATGATCTCTATGCAGATCATGATGAATCTGCCGAGAGTTTATGTGGTGGAGATGATGGTGGCTGTGAAGTGGATTATTATGACTCTGAAGATGAAGAAGGGCTTACTTTTGATACTGAATCATTTTCAAGATTTCTAATCCGGGTTCCATTGTCTGATACCAAGGTGTTTTCTCAGCTGGCTTTCCTGTCAAACATGGCATACGTTATACAAAAAATAAAGGCAGAGGATTTAGAAATATACCATGGCCTGCAATTTGTAACATCCTCCTTGCGCAAGAAAGCAGATGCTGCAGCCATTAATATCAAAGAAAAACTGGATCAGCACTCAACTTGTATTTCTGATGAGGCCTTCAATGCTGGTGAATCTTATTCAGTGGAAGCATTGGAGCTGGAGAGGACACGACAAATCCCTACAGCTGTTGCTTATGAGATTGCTGCGACAGCTGCATCTTATGTTCACTCGAGAGCCAAGAATCCTTCATCCCATCCTTTGGAATCACAAGAGAAGGGAGAGAGTTCGACTCGGGCATATAATCCCGAGGTGGCTGCCTATGTTGCAGCTTCAACAATGACGGCTGTGGTTGCTGCTGAGGAGGTGCAGAAACAGGAGACTGCAAAAGACCTCCAATCTCTTCACTCTTCTCCTTGTGAATGGTTTGGATCGGATTCCTTGGCATCTTGGCAAGCAAATCTTTTTTTTGAGCCCACCAAATTTGAGGGGACAGATGTGCTTGTCCATAGAGGAATATATGAAGCTGCAAAGGGAATATACAAGCAATTCATGCCAGAAATTATAGACCATTTGAAAAAATATGGAACCCATGCAAAGTTTCAGTTTACTGGCCATTCTCTTGGAGGCAGTCTTTCCCTTTTGGTGCATTTGATGCTACTAAAGAATGGGATTGTCAAACCTACTATGCTTCAACCAGTTGTCACTTTTGGCTCTCCATTTGTGTTCTGTGGTGGACACAAGATTCTAAATGAATTGGGTTTGGATGAAGACGATATCTATTGCATCATAATGCATCGAGATATCGTTCCAAGGGCCTTCTCCTGCAACTATCCCAACCATGTCGCTGCTGTACTCAAGCGGTTGAGTGGATCGTTTCGGTCTCATTCTTGTCTCAACAAAAGTAAATTACTATACACTCCATTGGGTAAGCTATTCATTTTGCAACCTGATGAGATGGCATCTCCCCCTCATCCTATGCTCCCTCTGGGAAGTGCCCTCTATACTTTGGATAGCACCCAAAATGGATACTCCAAAGGCCTCCTTAGGGCTTTTCTAAATTGCCCTCATCCCCTTGAAACACTCAGTGACCCTACAGCTTATGGGTCAGAGGGCACCATTTTAAGAGACCATGACTCAAGTTTCTATCTCAAGGCTTTGAATGGGGTTCTTAAACAACACACAAAGATGAATGTTGAGAAAGTCAGAAAGCAGAGGAAATTGTTATGGCCATTACTTGCTTCACCATCTCCAAAGTTGTGGAGCCATGATGGAAATTTGGACAATAATAATAGTTTGCTGTCCAATGAGATAATGACAGGTGTTTAAGACCATTTTCCATGAGCTCTGCCTTGCTTGTGTAGATGGATGGCTCACTTTTCAGCTTGGTTATCATCTTGTAAATCTCTTTTGTAAATGCATACAATAATATATCTTTCCAGAAAGGGAGAAAAAGGTATTTATTCTTATAGATCTTTTGTAAAATAGTTATACAAAAAAGATAGAGTAATTGGAGAGGAAAAAAAATATTGTTGATGAGCC

Coding sequence (CDS)

ATGGCTTGTACTTCAGTGGCTATTGGCAATTCTCCAGCCGCTGCAATGACAAAAGACATCATCTTCAAAGAATATAATGGGCTTCGCCGTTCGCATTCGAGCAAGGACGTTAGAGAACGAGCTTGCATCAAAAGATCTTATTCTGATAACCAGATTTGTTACTCTGTAAACAAAATTCATGCTACTTCAACACAGCCAAAGCCAAAAAACAACAATAACAGCTCTATGGGAATGGGGATTTTTCCTTTGAAATTTTCAGGGTCTTTTCTTCCTAATGCGGTCAGATCATTTTTGTTTGACATGGAGGAGACCTCAAAGGATCTCAGTAGTGAAGAGGAGGTGACAAAGAGAGCAAACTGGATCGAGAGATTGTTGGAGATTCGAAGTCGTTGGCGGAAAAAGCAGGAAAAAGGAGTTGAGAATGATCTCTATGCAGATCATGATGAATCTGCCGAGAGTTTATGTGGTGGAGATGATGGTGGCTGTGAAGTGGATTATTATGACTCTGAAGATGAAGAAGGGCTTACTTTTGATACTGAATCATTTTCAAGATTTCTAATCCGGGTTCCATTGTCTGATACCAAGGTGTTTTCTCAGCTGGCTTTCCTGTCAAACATGGCATACGTTATACAAAAAATAAAGGCAGAGGATTTAGAAATATACCATGGCCTGCAATTTGTAACATCCTCCTTGCGCAAGAAAGCAGATGCTGCAGCCATTAATATCAAAGAAAAACTGGATCAGCACTCAACTTGTATTTCTGATGAGGCCTTCAATGCTGGTGAATCTTATTCAGTGGAAGCATTGGAGCTGGAGAGGACACGACAAATCCCTACAGCTGTTGCTTATGAGATTGCTGCGACAGCTGCATCTTATGTTCACTCGAGAGCCAAGAATCCTTCATCCCATCCTTTGGAATCACAAGAGAAGGGAGAGAGTTCGACTCGGGCATATAATCCCGAGGTGGCTGCCTATGTTGCAGCTTCAACAATGACGGCTGTGGTTGCTGCTGAGGAGGTGCAGAAACAGGAGACTGCAAAAGACCTCCAATCTCTTCACTCTTCTCCTTGTGAATGGTTTGGATCGGATTCCTTGGCATCTTGGCAAGCAAATCTTTTTTTTGAGCCCACCAAATTTGAGGGGACAGATGTGCTTGTCCATAGAGGAATATATGAAGCTGCAAAGGGAATATACAAGCAATTCATGCCAGAAATTATAGACCATTTGAAAAAATATGGAACCCATGCAAAGTTTCAGTTTACTGGCCATTCTCTTGGAGGCAGTCTTTCCCTTTTGGTGCATTTGATGCTACTAAAGAATGGGATTGTCAAACCTACTATGCTTCAACCAGTTGTCACTTTTGGCTCTCCATTTGTGTTCTGTGGTGGACACAAGATTCTAAATGAATTGGGTTTGGATGAAGACGATATCTATTGCATCATAATGCATCGAGATATCGTTCCAAGGGCCTTCTCCTGCAACTATCCCAACCATGTCGCTGCTGTACTCAAGCGGTTGAGTGGATCGTTTCGGTCTCATTCTTGTCTCAACAAAAGTAAATTACTATACACTCCATTGGGTAAGCTATTCATTTTGCAACCTGATGAGATGGCATCTCCCCCTCATCCTATGCTCCCTCTGGGAAGTGCCCTCTATACTTTGGATAGCACCCAAAATGGATACTCCAAAGGCCTCCTTAGGGCTTTTCTAAATTGCCCTCATCCCCTTGAAACACTCAGTGACCCTACAGCTTATGGGTCAGAGGGCACCATTTTAAGAGACCATGACTCAAGTTTCTATCTCAAGGCTTTGAATGGGGTTCTTAAACAACACACAAAGATGAATGTTGAGAAAGTCAGAAAGCAGAGGAAATTGTTATGGCCATTACTTGCTTCACCATCTCCAAAGTTGTGGAGCCATGATGGAAATTTGGACAATAATAATAGTTTGCTGTCCAATGAGATAATGACAGGTGTTTAA

Protein sequence

MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIHATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRANWIERLLEIRSRWRKKQEKGVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDTESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAAAINIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAKNPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEWFGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPEIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGHKILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYTPLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGNLDNNNSLLSNEIMTGV
Homology
BLAST of Lsi02G020250 vs. ExPASy Swiss-Prot
Match: Q7Y220 (Phospholipase A1 PLIP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP1 PE=1 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 3.5e-171
Identity = 350/690 (50.72%), Postives = 453/690 (65.65%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKD-VRERACIKRSYSDNQIC----YS 60
           MA  +     SPAAA   + + +E+ GLRRS S +D V +   I+RS SDN +C     +
Sbjct: 1   MAFNTAMASTSPAAA---NDVLREHIGLRRSLSGQDLVLKGGGIRRSSSDNHLCCRSGNN 60

Query: 61  VNKIHATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEV- 120
            N+I A S +P  K    +S  +G+F  + S S +P+ +++ LF+  +TS+D    +E+ 
Sbjct: 61  NNRILAVSVRPGMK----TSRSVGVFSFQISSSIIPSPIKTLLFE-TDTSQDEQESDEIE 120

Query: 121 ---------TKRANWIERLLEIRSRWRKKQEKGVENDLYADHDESAESLCG-GDDGGCEV 180
                     K+ANW+ERLLEIR +W+++Q+    N   A  +ES +  CG  ++ GC  
Sbjct: 121 IETEPNLDGAKKANWVERLLEIRRQWKREQKTESGNSDVA--EESVDVTCGCEEEEGCIA 180

Query: 181 DYYDSEDEEGLTFDTESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGL 240
           +Y     + G     ESFSR L++V  S+ K  SQLA+L N+AY I +IK EDL   +GL
Sbjct: 181 NYGSVNGDWG----RESFSRLLVKVSWSEAKKLSQLAYLCNLAYTIPEIKGEDLRRNYGL 240

Query: 241 QFVTSSLRKKADAAAINIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYE 300
           +FVTSSL KKA AA   ++EKL+Q  T +         S  +E+ +  +     +A AY+
Sbjct: 241 KFVTSSLEKKAKAAI--LREKLEQDPTHVP-----VITSPDLESEKQSQRSASSSASAYK 300

Query: 301 IAATAASYVHSRAKNPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQE 360
           IAA+AASY+HS  +   S P+            Y    AA  AASTMTAVVAA E +K E
Sbjct: 301 IAASAASYIHSCKEYDLSEPI------------YKSAAAAQAAASTMTAVVAAGEEEKLE 360

Query: 361 TAKDLQSLHSSPCEWF---------------GSDSLASWQANLFFEPTKFEGTDVLVHRG 420
            A++LQSL SSPCEWF               GSDSLASW+ANLFFEPTKFE TDVLVHRG
Sbjct: 361 AARELQSLQSSPCEWFVCDDPNTYTRCFVIQGSDSLASWKANLFFEPTKFEDTDVLVHRG 420

Query: 421 IYEAAKGIYKQFMPEIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQ 480
           IYEAAKGIY+QF+PEI +HL ++G  AKFQFTGHSLGGSLSL+V+LML+  G+V    ++
Sbjct: 421 IYEAAKGIYEQFLPEITEHLSRHGDRAKFQFTGHSLGGSLSLIVNLMLISRGLVSSEAMK 480

Query: 481 PVVTFGSPFVFCGGHKILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGS 540
            VVTFGSPFVFCGG KIL ELGLDE  ++C++MHRDIVPRAFSCNYP+HVA VLKRL+GS
Sbjct: 481 SVVTFGSPFVFCGGEKILAELGLDESHVHCVMMHRDIVPRAFSCNYPDHVALVLKRLNGS 540

Query: 541 FRSHSCLNKSKLLYTPLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAF 600
           FR+H CLNK+KLLY+P+GK++ILQP E  SP HP LP G+ALY L+++  GYS   LRAF
Sbjct: 541 FRTHPCLNKNKLLYSPMGKVYILQPSESVSPTHPWLPPGNALYILENSNEGYSPTALRAF 600

Query: 601 LNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPL 660
           LN PHPLETLS   AYGSEG++LRDHDS  Y+KA+NGVL+QHTK+ V K R QR+ +WP+
Sbjct: 601 LNRPHPLETLSQRAAYGSEGSVLRDHDSKNYVKAVNGVLRQHTKLIVRKARIQRRSVWPV 649

BLAST of Lsi02G020250 vs. ExPASy Swiss-Prot
Match: F4HXL0 (Phospholipase A1 PLIP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP2 PE=1 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 3.8e-125
Identity = 276/635 (43.46%), Postives = 376/635 (59.21%), Query Frame = 0

Query: 53  CYSVNKIHATSTQPKPKNNN-------------NSSMGMGIFPLKFSGSFLPNAVRSFLF 112
           C  V ++ AT++ P  K                +   G GI   + SG  L +AV   L 
Sbjct: 25  CGGVVEVRATASAPSQKRGPFGFSFKYPLTPFWSRGGGGGIASRRRSGLCLDDAV---LV 84

Query: 113 DMEETSKDLSSEEEV---TKRAN--WIERLLEIRSRWRKKQEKGVE--NDLYADHDESAE 172
           D  ++ K ++ E  V   T+R N  W+ ++L+++S W+ ++E+  +   D   D DE  E
Sbjct: 85  DSGDSRKPIAEETAVEMDTERRNGSWVLKILDVQSTWKHEEEEDDDEVEDEDGDEDEEVE 144

Query: 173 ---SLCGGDDGGCEVDYY---DSEDEEGLTFDTESFSRFLIRVPLSDTKVFSQLAFLSNM 232
              ++   DDGGC+V      D  +      D ESFS+ L RV L ++K+++QL++L N+
Sbjct: 145 LDDAVVSEDDGGCDVCSVLEDDGNEANKFQLDRESFSKLLRRVTLPESKLYAQLSYLGNL 204

Query: 233 AYVIQKIKAEDLEIYHGLQFVTSSLRKKADAAAINIKEKLDQHSTCISDEAFNAGESYSV 292
           AY I KIK  +L  Y+GL+FVTSS  K   A      E   +    +  E         V
Sbjct: 205 AYSISKIKPANLSKYYGLRFVTSSAEKTESALKAENGEVSGETKPIVEAEE-------EV 264

Query: 293 EALELERTRQIPTAVAYEIAATAASYVHSRAKNPSSHPLESQEKGESSTR----AYNPEV 352
           E  E  ++R+I  + AYEI A+AASY+HSR  N    P  S  K E+S +      N E 
Sbjct: 265 EEEEKNKSRKISASAAYEIVASAASYLHSRTNN--ILPFNSSSKAENSDKHDVNLTNAES 324

Query: 353 AAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEWF---------------GSDSLAS 412
           ++ VA S +T+VVAAEE  KQ  A DL+S  SSPC+WF               GS+SLAS
Sbjct: 325 SSDVAYS-VTSVVAAEEDVKQAVADDLKSTISSPCDWFICDDDQSHTRFVVIQGSESLAS 384

Query: 413 WQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPEIIDHLKKYGTHAKFQFTGHSLGG 472
           WQANL FEP +FEG   +VHRGIYEAAKG+Y+Q +PE+  H+K +GT AKF+FTGHSLGG
Sbjct: 385 WQANLLFEPIEFEGLGAIVHRGIYEAAKGMYEQMLPEVKAHIKTHGTSAKFRFTGHSLGG 444

Query: 473 SLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGHKILNELGLDEDDIYCIIMHRDIV 532
           SLSLL++LMLL  G V  + L PV+T+G+PFV CGG ++L +LGL +  +  I+MHRDIV
Sbjct: 445 SLSLLLNLMLLVRGEVPASSLLPVITYGAPFVLCGGDRLLKKLGLPKSHVQAIVMHRDIV 504

Query: 533 PRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYTPLGKLFILQPDEMASPPHPMLPL 592
           PRAFSCNYP HVA +LK ++G+FRSH CLNK  +LY+P+G+L ILQPDE  SP H +LP 
Sbjct: 505 PRAFSCNYPYHVAELLKAVNGNFRSHPCLNKQSMLYSPMGELLILQPDETFSPGHELLPS 564

Query: 593 GSALYTLDS---------TQNGYSKGLLRAFLNCPHPLETLSDPTAYGSEGTILRDHDSS 633
           G+ LY L S         +     +     FLN PHPL+ LSD +AYGS GTI RDHD +
Sbjct: 565 GNGLYLLTSDFESPDIEDSDEERLRAAQTVFLNTPHPLDILSDRSAYGSSGTIQRDHDMN 624

BLAST of Lsi02G020250 vs. ExPASy Swiss-Prot
Match: Q940L4 (Phospholipase A1 PLIP3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP3 PE=1 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.1e-111
Identity = 249/534 (46.63%), Postives = 321/534 (60.11%), Query Frame = 0

Query: 119 NWIERLLEIRSRWR-KKQEKGVENDLYADHDESA------ESLCGGDDGGCEVDYYDSED 178
           NW+ ++LE+ S W+ K+Q  G       D +E        E LC   D  C +D  D ++
Sbjct: 90  NWVLKILEVGSIWKGKRQRSGGGGGGEEDEEEEVAEPKKKEDLCEECD-FCRIDDDDEDE 149

Query: 179 EEGLTFDTESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSL 238
           E+  T     FS  L ++P+ D ++F++L+FL N+AY I KIK E+L  Y  L+FVTSS+
Sbjct: 150 EKEKT--VFEFSEMLSKIPVEDAQMFAKLSFLGNLAYSIPKIKPENLLKYQKLRFVTSSI 209

Query: 239 RKKADAAAINIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAAS 298
            K+       +  K+++++    DE               E+ + I  AVAY IAA+AAS
Sbjct: 210 EKR-------MSLKVEENNNGEEDE---------------EKKKLINPAVAYRIAASAAS 269

Query: 299 YVHSRAKNPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQS 358
            + S +K        S     SS R  N E +    A ++TAVVAA+E  KQ  A DL+S
Sbjct: 270 RLFSHSK--------SVLPFGSSKRQDNEEASLLATADSVTAVVAAKEEVKQAVADDLKS 329

Query: 359 LHSSPCEWF---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKG 418
             S PCEWF               GSDSLASWQANL FEP  FE  DVLVHRGIYEAAKG
Sbjct: 330 NRSPPCEWFVCDDDKSGTRFFFIQGSDSLASWQANLLFEPVPFEDLDVLVHRGIYEAAKG 389

Query: 419 IYKQFMPEIIDHLKKYGTHAKF-QFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFG 478
           IY+Q +PE+  HL   G +  F +F+GHSLGGSLSLLV+LMLL  G V  + L PV+TFG
Sbjct: 390 IYEQMLPEVHAHLNSRGKNRAFLRFSGHSLGGSLSLLVNLMLLIRGQVPASSLLPVITFG 449

Query: 479 SPFVFCGGHKILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSC 538
           SP + CGG ++L +LGL +  +  I MHRDIVPRAFSCNYPN  A +LK L+G+FR+H C
Sbjct: 450 SPCIMCGGDRLLQKLGLPKSHLLGISMHRDIVPRAFSCNYPNRAAKLLKALNGNFRNHPC 509

Query: 539 LNKSKLLYTPLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRA----FLN 598
           LN   +LY+P+GKL ILQP E  SPPHP+LP GS LY L S     ++  LRA    F N
Sbjct: 510 LNNQNVLYSPMGKLLILQPSERFSPPHPLLPPGSGLYLLASKNTDETEKSLRAAKILFFN 569

Query: 599 CPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLKQHTK-MNVEKVRKQRK 625
            PHPLE LSD  +YGSEG I R+HD S YLKAL  V+++  K M  E+ +  RK
Sbjct: 570 SPHPLEILSDRRSYGSEGKIKRNHDMSSYLKALRHVIRKELKQMKAERDQWLRK 590

BLAST of Lsi02G020250 vs. ExPASy TrEMBL
Match: A0A0A0K4E8 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G325710 PE=4 SV=1)

HSP 1 Score: 1218.0 bits (3150), Expect = 0.0e+00
Identity = 615/675 (91.11%), Postives = 638/675 (94.52%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNSPAA+MTK+IIFKEY+ LRRSHSSKDVRERACIKRSYSDNQICYS NKIH
Sbjct: 38  MACTSVAIGNSPAASMTKEIIFKEYSMLRRSHSSKDVRERACIKRSYSDNQICYSANKIH 97

Query: 61  ATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRANW 120
           ATSTQPKPKN NN+S GMGIFPLKFSGSFLPNAVRSFLFDMEETSK+L++EEEVTKRANW
Sbjct: 98  ATSTQPKPKNKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTTEEEVTKRANW 157

Query: 121 IERLLEIRSRWRKKQEK-GVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT 180
           IERLLEIRSRWR KQ+K G+ENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT
Sbjct: 158 IERLLEIRSRWRLKQQKGGLENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT 217

Query: 181 ESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAAA 240
           ESF+R+LIRVP+SDTKVFSQLAFLSNMAYVI  IKAEDLEIYHGLQFVTSSL KKA+AAA
Sbjct: 218 ESFARYLIRVPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLQFVTSSLHKKAEAAA 277

Query: 241 INIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAKN 300
           INIKEKLDQHSTCISDEAFN GES SVEAL+LERT++IPT VAYEIAATAASYVHSR KN
Sbjct: 278 INIKEKLDQHSTCISDEAFNVGESCSVEALKLERTQEIPTTVAYEIAATAASYVHSRVKN 337

Query: 301 PSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEW 360
            SSHPLESQEKG+ STR YNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCEW
Sbjct: 338 TSSHPLESQEKGDGSTRVYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCEW 397

Query: 361 F---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420
           F               GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE
Sbjct: 398 FVCDDTDTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 457

Query: 421 IIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480
           IIDHLKKYG HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH
Sbjct: 458 IIDHLKKYGAHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 517

Query: 481 KILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYT 540
           KILNELGLDED+I+CIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY+
Sbjct: 518 KILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYS 577

Query: 541 PLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600
           PLGKLFILQPDEM+SPPHPMLP GSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA
Sbjct: 578 PLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 637

Query: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGNL 660
           YGSEGTILRDHDSSFYLKALNGVLKQHTKM V KVR QRKLLWPLLASPSP+LWSHDGNL
Sbjct: 638 YGSEGTILRDHDSSFYLKALNGVLKQHTKMAVGKVRNQRKLLWPLLASPSPELWSHDGNL 697

BLAST of Lsi02G020250 vs. ExPASy TrEMBL
Match: A0A1S4E386 (uncharacterized protein LOC103500094 OS=Cucumis melo OX=3656 GN=LOC103500094 PE=4 SV=1)

HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 612/675 (90.67%), Postives = 635/675 (94.07%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNSP A M K+IIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYS NKI+
Sbjct: 1   MACTSVAIGNSPVATMAKEIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSANKIY 60

Query: 61  ATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRANW 120
           ATSTQPKPK  NN+S GMGIFPLKFSGSFLPNAVRSFLFDMEETSK+L+SEEEVTKRANW
Sbjct: 61  ATSTQPKPKIKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTSEEEVTKRANW 120

Query: 121 IERLLEIRSRWRKKQEK-GVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT 180
           IERLLEIRSRWR KQ+K G+ENDLYADHDESAESLCGG+DGGCEVDYYDSEDEEGLTFDT
Sbjct: 121 IERLLEIRSRWRLKQQKGGLENDLYADHDESAESLCGGEDGGCEVDYYDSEDEEGLTFDT 180

Query: 181 ESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAAA 240
           ESF+R+LIR+P+SDTKVFSQLAFLSNMAYVI  IKAEDLEIYHGL+FVTSSLRKKA+AAA
Sbjct: 181 ESFARYLIRIPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLKFVTSSLRKKAEAAA 240

Query: 241 INIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAKN 300
           INIKEKLDQHSTCISDEAFN GES SVEA E+ERT++IPT VAYEIAATAASYVHSRAKN
Sbjct: 241 INIKEKLDQHSTCISDEAFNVGESCSVEASEVERTQEIPTTVAYEIAATAASYVHSRAKN 300

Query: 301 PSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEW 360
            SSHPLESQEKG+ STRAYNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCEW
Sbjct: 301 TSSHPLESQEKGDGSTRAYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCEW 360

Query: 361 F---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420
           F               GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE
Sbjct: 361 FVCDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420

Query: 421 IIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480
           IIDHLKKYG HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH
Sbjct: 421 IIDHLKKYGDHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480

Query: 481 KILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYT 540
           KILNELGLDED+I+CIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY+
Sbjct: 481 KILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYS 540

Query: 541 PLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600
           PLGKLFILQPDEM+SPPHPMLP GSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA
Sbjct: 541 PLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600

Query: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGNL 660
           YGSEGTILRDHDSSFYLKALNGVLKQHTKM V KVR QRKLLWPLLASPSP  WSHDGNL
Sbjct: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMGVGKVRNQRKLLWPLLASPSPDRWSHDGNL 660

BLAST of Lsi02G020250 vs. ExPASy TrEMBL
Match: A0A5D3BZ41 (Lipase_3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold675G00720 PE=4 SV=1)

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 612/675 (90.67%), Postives = 635/675 (94.07%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNSP A M K+IIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYS NKI+
Sbjct: 1   MACTSVAIGNSPVATMAKEIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSANKIY 60

Query: 61  ATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRANW 120
           ATSTQPKPK  NN+S GMGIFPLKFSGSFLPNAVRSFLFDMEETSK+L+SEEEVTKRANW
Sbjct: 61  ATSTQPKPKIKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTSEEEVTKRANW 120

Query: 121 IERLLEIRSRWRKKQEK-GVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT 180
           IERLLEIRSRWR KQ+K G+ENDLYAD DESAESLCGG+DGGCEVDYYDSEDEEGLTFDT
Sbjct: 121 IERLLEIRSRWRLKQQKGGLENDLYAD-DESAESLCGGEDGGCEVDYYDSEDEEGLTFDT 180

Query: 181 ESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAAA 240
           ESF+R+LIR+P+SDTKVFSQLAFLSNMAYVI  IKAEDLEIYHGL+FVTSSLRKKA+AAA
Sbjct: 181 ESFARYLIRIPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLKFVTSSLRKKAEAAA 240

Query: 241 INIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAKN 300
           INIKEKLDQHSTCISDEAFN GES SVEALE+ERT++IPT VAYEIAATAASYVHSRAKN
Sbjct: 241 INIKEKLDQHSTCISDEAFNVGESCSVEALEVERTQEIPTTVAYEIAATAASYVHSRAKN 300

Query: 301 PSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEW 360
            SSHPLESQEKG+ STRAYNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCEW
Sbjct: 301 TSSHPLESQEKGDGSTRAYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCEW 360

Query: 361 F---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420
           F               GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE
Sbjct: 361 FVCDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420

Query: 421 IIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480
           IIDHLKKYG HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH
Sbjct: 421 IIDHLKKYGDHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480

Query: 481 KILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYT 540
           KILNELGLDED+I+CIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY+
Sbjct: 481 KILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYS 540

Query: 541 PLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600
           PLGKLFILQPDEM+SPPHPMLP GSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA
Sbjct: 541 PLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600

Query: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGNL 660
           YGSEGTILRDHDSSFYLKALNGVLKQHTKM V KVR QRKLLWPLLASPSP  WSHDGNL
Sbjct: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMGVGKVRNQRKLLWPLLASPSPDRWSHDGNL 660

BLAST of Lsi02G020250 vs. ExPASy TrEMBL
Match: A0A6J1JSU1 (uncharacterized protein LOC111488580 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488580 PE=4 SV=1)

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 574/676 (84.91%), Postives = 610/676 (90.24%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNS A +MTKDIIFKEYNG+RRSHSSKD+RER CIKRSYSDN ICYS NKIH
Sbjct: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60

Query: 61  ATSTQPKPK--NNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRA 120
           ATSTQPKPK  NNNN+SMGM IFPLKFSGSFLP+ +RSFLFDMEETSKDLSSEEEVT+RA
Sbjct: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120

Query: 121 NWIERLLEIRSRWRKKQEKGVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFD 180
           NWIERLLEIR  WR KQ+KGVEND+Y  HDE+ E  CG DDGGCEVDYYDSEDE   +FD
Sbjct: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180

Query: 181 TESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAA 240
           TESF+RFL++VPLSDTKVFSQLAFLSNMAYVI +IKAE+LE   GL+FVTSSLRKKA+ A
Sbjct: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240

Query: 241 AINIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAK 300
           A N+KEK DQHSTCISDEA N GE  S EAL+LERT+QIPT VAYEIAATAASY+HSRAK
Sbjct: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300

Query: 301 NPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           NPSSHPLE QE+GESS RAYNPEVAAYVAASTMTAVVAAEEVQKQETAK LQSLHSSPCE
Sbjct: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360

Query: 361 WF---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WF               GSDSLASWQANLFFEPTKFEG+DVLVHRGIYEAAKGIYKQF+P
Sbjct: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420

Query: 421 EIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 480
           EII+HLK YGTHAKFQFTGHSLGGSLSLLVHLMLLKN IVKP MLQPVVTFGSPFVFCGG
Sbjct: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480

Query: 481 HKILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKIL ELGLDEDDI C+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 TPLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600
           +P+GK+FILQP+EM+SPPHPMLP GSALYTLDSTQNGYS G+LRAFLNCPHPLETLSDPT
Sbjct: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVL QHTKM+  KVRKQRKLL PLL SPSP+LW HDGN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660

BLAST of Lsi02G020250 vs. ExPASy TrEMBL
Match: A0A6J1JUY6 (uncharacterized protein LOC111488580 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488580 PE=4 SV=1)

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 574/676 (84.91%), Postives = 610/676 (90.24%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNS A +MTKDIIFKEYNG+RRSHSSKD+RER CIKRSYSDN ICYS NKIH
Sbjct: 34  MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 93

Query: 61  ATSTQPKPK--NNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRA 120
           ATSTQPKPK  NNNN+SMGM IFPLKFSGSFLP+ +RSFLFDMEETSKDLSSEEEVT+RA
Sbjct: 94  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 153

Query: 121 NWIERLLEIRSRWRKKQEKGVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFD 180
           NWIERLLEIR  WR KQ+KGVEND+Y  HDE+ E  CG DDGGCEVDYYDSEDE   +FD
Sbjct: 154 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 213

Query: 181 TESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAA 240
           TESF+RFL++VPLSDTKVFSQLAFLSNMAYVI +IKAE+LE   GL+FVTSSLRKKA+ A
Sbjct: 214 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 273

Query: 241 AINIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAK 300
           A N+KEK DQHSTCISDEA N GE  S EAL+LERT+QIPT VAYEIAATAASY+HSRAK
Sbjct: 274 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 333

Query: 301 NPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           NPSSHPLE QE+GESS RAYNPEVAAYVAASTMTAVVAAEEVQKQETAK LQSLHSSPCE
Sbjct: 334 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 393

Query: 361 WF---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WF               GSDSLASWQANLFFEPTKFEG+DVLVHRGIYEAAKGIYKQF+P
Sbjct: 394 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 453

Query: 421 EIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 480
           EII+HLK YGTHAKFQFTGHSLGGSLSLLVHLMLLKN IVKP MLQPVVTFGSPFVFCGG
Sbjct: 454 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 513

Query: 481 HKILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKIL ELGLDEDDI C+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 514 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 573

Query: 541 TPLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600
           +P+GK+FILQP+EM+SPPHPMLP GSALYTLDSTQNGYS G+LRAFLNCPHPLETLSDPT
Sbjct: 574 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 633

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVL QHTKM+  KVRKQRKLL PLL SPSP+LW HDGN
Sbjct: 634 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 693

BLAST of Lsi02G020250 vs. NCBI nr
Match: XP_004139735.1 (phospholipase A1 PLIP1, chloroplastic [Cucumis sativus] >KGN44827.2 hypothetical protein Csa_015640 [Cucumis sativus])

HSP 1 Score: 1218.0 bits (3150), Expect = 0.0e+00
Identity = 615/675 (91.11%), Postives = 638/675 (94.52%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNSPAA+MTK+IIFKEY+ LRRSHSSKDVRERACIKRSYSDNQICYS NKIH
Sbjct: 1   MACTSVAIGNSPAASMTKEIIFKEYSMLRRSHSSKDVRERACIKRSYSDNQICYSANKIH 60

Query: 61  ATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRANW 120
           ATSTQPKPKN NN+S GMGIFPLKFSGSFLPNAVRSFLFDMEETSK+L++EEEVTKRANW
Sbjct: 61  ATSTQPKPKNKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTTEEEVTKRANW 120

Query: 121 IERLLEIRSRWRKKQEK-GVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT 180
           IERLLEIRSRWR KQ+K G+ENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT
Sbjct: 121 IERLLEIRSRWRLKQQKGGLENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT 180

Query: 181 ESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAAA 240
           ESF+R+LIRVP+SDTKVFSQLAFLSNMAYVI  IKAEDLEIYHGLQFVTSSL KKA+AAA
Sbjct: 181 ESFARYLIRVPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLQFVTSSLHKKAEAAA 240

Query: 241 INIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAKN 300
           INIKEKLDQHSTCISDEAFN GES SVEAL+LERT++IPT VAYEIAATAASYVHSR KN
Sbjct: 241 INIKEKLDQHSTCISDEAFNVGESCSVEALKLERTQEIPTTVAYEIAATAASYVHSRVKN 300

Query: 301 PSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEW 360
            SSHPLESQEKG+ STR YNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCEW
Sbjct: 301 TSSHPLESQEKGDGSTRVYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCEW 360

Query: 361 F---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420
           F               GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE
Sbjct: 361 FVCDDTDTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420

Query: 421 IIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480
           IIDHLKKYG HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH
Sbjct: 421 IIDHLKKYGAHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480

Query: 481 KILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYT 540
           KILNELGLDED+I+CIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY+
Sbjct: 481 KILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYS 540

Query: 541 PLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600
           PLGKLFILQPDEM+SPPHPMLP GSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA
Sbjct: 541 PLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600

Query: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGNL 660
           YGSEGTILRDHDSSFYLKALNGVLKQHTKM V KVR QRKLLWPLLASPSP+LWSHDGNL
Sbjct: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMAVGKVRNQRKLLWPLLASPSPELWSHDGNL 660

BLAST of Lsi02G020250 vs. NCBI nr
Match: XP_038897374.1 (phospholipase A1 PLIP1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 616/676 (91.12%), Postives = 635/676 (93.93%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNSPAA +TKDIIFKEYNGLRRSHSSKD+RERACIKRSYSDNQICYS NKIH
Sbjct: 1   MACTSVAIGNSPAATITKDIIFKEYNGLRRSHSSKDIRERACIKRSYSDNQICYSANKIH 60

Query: 61  ATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRANW 120
           A STQPKPKN+NN+SMG GIFPLKFSGSFLPNAVRSFLFDMEETSKDL+ EEEVTKRANW
Sbjct: 61  ANSTQPKPKNSNNNSMGTGIFPLKFSGSFLPNAVRSFLFDMEETSKDLTGEEEVTKRANW 120

Query: 121 IERLLEIRSRWRKKQEK--GVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFD 180
           IERLLEIRSRWR KQ++  GVENDLYADHDE  ES+ GGDD  CEVDYYDSEDEEGLTFD
Sbjct: 121 IERLLEIRSRWRIKQQQKGGVENDLYADHDEYTESVFGGDDDVCEVDYYDSEDEEGLTFD 180

Query: 181 TESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAA 240
           TE F+RFLIRVPLSDTKVFS+LAFLSNMAYVI KIKAEDLEIYHGLQFVTSSLRKKADAA
Sbjct: 181 TELFARFLIRVPLSDTKVFSRLAFLSNMAYVIPKIKAEDLEIYHGLQFVTSSLRKKADAA 240

Query: 241 AINIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAK 300
           AINIKEKLDQ STCISDEAFN  ES+SVEALEL RTRQIPT VAYEIAATAASYVHSRAK
Sbjct: 241 AINIKEKLDQQSTCISDEAFNVDESFSVEALELGRTRQIPTTVAYEIAATAASYVHSRAK 300

Query: 301 NPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           NPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE
Sbjct: 301 NPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360

Query: 361 WF---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WF               GSDSLASWQANLFFEPTKFEGT+VLVHRGIYEAAKGIYKQFMP
Sbjct: 361 WFICDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTEVLVHRGIYEAAKGIYKQFMP 420

Query: 421 EIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 480
           EIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNG+VKPTMLQPVVTFGSPFVFCGG
Sbjct: 421 EIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGVVKPTMLQPVVTFGSPFVFCGG 480

Query: 481 HKILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILNELGLDE+DI+C+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILNELGLDENDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 TPLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600
           +PLGKLFILQPDEM+SPPHPMLP GSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT
Sbjct: 541 SPLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVLKQ TKM V KVR QRKLLWPLLASPSP+LWSHDGN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLKQQTKMTVGKVRNQRKLLWPLLASPSPELWSHDGN 660

BLAST of Lsi02G020250 vs. NCBI nr
Match: XP_008461523.1 (PREDICTED: uncharacterized protein LOC103500094 [Cucumis melo] >XP_016902691.1 PREDICTED: uncharacterized protein LOC103500094 [Cucumis melo] >XP_016902692.1 PREDICTED: uncharacterized protein LOC103500094 [Cucumis melo])

HSP 1 Score: 1211.8 bits (3134), Expect = 0.0e+00
Identity = 612/675 (90.67%), Postives = 635/675 (94.07%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNSP A M K+IIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYS NKI+
Sbjct: 1   MACTSVAIGNSPVATMAKEIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSANKIY 60

Query: 61  ATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRANW 120
           ATSTQPKPK  NN+S GMGIFPLKFSGSFLPNAVRSFLFDMEETSK+L+SEEEVTKRANW
Sbjct: 61  ATSTQPKPKIKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTSEEEVTKRANW 120

Query: 121 IERLLEIRSRWRKKQEK-GVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT 180
           IERLLEIRSRWR KQ+K G+ENDLYADHDESAESLCGG+DGGCEVDYYDSEDEEGLTFDT
Sbjct: 121 IERLLEIRSRWRLKQQKGGLENDLYADHDESAESLCGGEDGGCEVDYYDSEDEEGLTFDT 180

Query: 181 ESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAAA 240
           ESF+R+LIR+P+SDTKVFSQLAFLSNMAYVI  IKAEDLEIYHGL+FVTSSLRKKA+AAA
Sbjct: 181 ESFARYLIRIPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLKFVTSSLRKKAEAAA 240

Query: 241 INIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAKN 300
           INIKEKLDQHSTCISDEAFN GES SVEA E+ERT++IPT VAYEIAATAASYVHSRAKN
Sbjct: 241 INIKEKLDQHSTCISDEAFNVGESCSVEASEVERTQEIPTTVAYEIAATAASYVHSRAKN 300

Query: 301 PSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEW 360
            SSHPLESQEKG+ STRAYNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCEW
Sbjct: 301 TSSHPLESQEKGDGSTRAYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCEW 360

Query: 361 F---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420
           F               GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE
Sbjct: 361 FVCDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420

Query: 421 IIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480
           IIDHLKKYG HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH
Sbjct: 421 IIDHLKKYGDHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480

Query: 481 KILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYT 540
           KILNELGLDED+I+CIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY+
Sbjct: 481 KILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYS 540

Query: 541 PLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600
           PLGKLFILQPDEM+SPPHPMLP GSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA
Sbjct: 541 PLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600

Query: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGNL 660
           YGSEGTILRDHDSSFYLKALNGVLKQHTKM V KVR QRKLLWPLLASPSP  WSHDGNL
Sbjct: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMGVGKVRNQRKLLWPLLASPSPDRWSHDGNL 660

BLAST of Lsi02G020250 vs. NCBI nr
Match: KAA0051002.1 (uncharacterized protein E6C27_scaffold2606G00220 [Cucumis melo var. makuwa] >TYK04390.1 uncharacterized protein E5676_scaffold675G00720 [Cucumis melo var. makuwa])

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 612/675 (90.67%), Postives = 635/675 (94.07%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNSP A M K+IIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYS NKI+
Sbjct: 1   MACTSVAIGNSPVATMAKEIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSANKIY 60

Query: 61  ATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRANW 120
           ATSTQPKPK  NN+S GMGIFPLKFSGSFLPNAVRSFLFDMEETSK+L+SEEEVTKRANW
Sbjct: 61  ATSTQPKPKIKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTSEEEVTKRANW 120

Query: 121 IERLLEIRSRWRKKQEK-GVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFDT 180
           IERLLEIRSRWR KQ+K G+ENDLYAD DESAESLCGG+DGGCEVDYYDSEDEEGLTFDT
Sbjct: 121 IERLLEIRSRWRLKQQKGGLENDLYAD-DESAESLCGGEDGGCEVDYYDSEDEEGLTFDT 180

Query: 181 ESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAAA 240
           ESF+R+LIR+P+SDTKVFSQLAFLSNMAYVI  IKAEDLEIYHGL+FVTSSLRKKA+AAA
Sbjct: 181 ESFARYLIRIPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLKFVTSSLRKKAEAAA 240

Query: 241 INIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAKN 300
           INIKEKLDQHSTCISDEAFN GES SVEALE+ERT++IPT VAYEIAATAASYVHSRAKN
Sbjct: 241 INIKEKLDQHSTCISDEAFNVGESCSVEALEVERTQEIPTTVAYEIAATAASYVHSRAKN 300

Query: 301 PSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEW 360
            SSHPLESQEKG+ STRAYNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCEW
Sbjct: 301 TSSHPLESQEKGDGSTRAYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCEW 360

Query: 361 F---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420
           F               GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE
Sbjct: 361 FVCDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPE 420

Query: 421 IIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480
           IIDHLKKYG HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH
Sbjct: 421 IIDHLKKYGDHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGH 480

Query: 481 KILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYT 540
           KILNELGLDED+I+CIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY+
Sbjct: 481 KILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYS 540

Query: 541 PLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600
           PLGKLFILQPDEM+SPPHPMLP GSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA
Sbjct: 541 PLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPTA 600

Query: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGNL 660
           YGSEGTILRDHDSSFYLKALNGVLKQHTKM V KVR QRKLLWPLLASPSP  WSHDGNL
Sbjct: 601 YGSEGTILRDHDSSFYLKALNGVLKQHTKMGVGKVRNQRKLLWPLLASPSPDRWSHDGNL 660

BLAST of Lsi02G020250 vs. NCBI nr
Match: XP_023547682.1 (uncharacterized protein LOC111806547 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 578/676 (85.50%), Postives = 611/676 (90.38%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSVNKIH 60
           MACTSVAIGNS A +MTKDIIFKEYNG+RRSHSSKD+RER CIKRSYSDN ICYS NKIH
Sbjct: 34  MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 93

Query: 61  ATSTQPKPK--NNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEVTKRA 120
           ATSTQPKPK  NNNN+SMGM IFPLKFSGSFLP+ +RSFLFDMEETSKDLSSEEEVT+RA
Sbjct: 94  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 153

Query: 121 NWIERLLEIRSRWRKKQEKGVENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFD 180
           NWIERLLEIR  WR KQ+KGVEND+YA HDE+ E  CG DDGGCEVDYYDSEDE   +FD
Sbjct: 154 NWIERLLEIRRHWRTKQKKGVENDIYAHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 213

Query: 181 TESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSLRKKADAA 240
           TESF+RFL++VPLSDTKVFSQLAFLSNMAYVI +IKAE+LE   GL+FVTSSL KKA+ A
Sbjct: 214 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERSCGLKFVTSSLLKKANGA 273

Query: 241 AINIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAASYVHSRAK 300
           AIN+KEK DQHSTCISDEA N GE  S EAL+LERT+QIPT VAYEIAATAASY+HSRAK
Sbjct: 274 AINMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTNVAYEIAATAASYMHSRAK 333

Query: 301 NPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           NPSSHPLE QEKGE S RAYNPEVAAYVAASTMTAVVAAEEVQKQETAK LQSLHSSPCE
Sbjct: 334 NPSSHPLEPQEKGEGSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 393

Query: 361 WF---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WF               GSDSLASWQANLFFEPTKFEG+DVLVHRGIYEAAKGIYKQF+P
Sbjct: 394 WFVCDDIHSPTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 453

Query: 421 EIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 480
           EII+HLKKYGT AKFQFTGHSLGGSLSLLVHLMLLKN IVKP MLQPVVTFGSPFVFCGG
Sbjct: 454 EIIEHLKKYGTQAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 513

Query: 481 HKILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILNELGLDEDDI C+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 514 HKILNELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 573

Query: 541 TPLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600
           +PLGK+FILQP+EM+SPPHPMLP GSALYTLDSTQNGYS GLLRAFLNCPHPLETLSDPT
Sbjct: 574 SPLGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSIGLLRAFLNCPHPLETLSDPT 633

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPLLASPSPKLWSHDGN 660
           AYGSEGTILRDHDSSFYLKALNGVL QHTKM+  KVRKQRKLL PLL SPSP+LW HDGN
Sbjct: 634 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTSKVRKQRKLLLPLLTSPSPELWRHDGN 693

BLAST of Lsi02G020250 vs. TAIR 10
Match: AT3G61680.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 603.2 bits (1554), Expect = 2.5e-172
Identity = 350/690 (50.72%), Postives = 453/690 (65.65%), Query Frame = 0

Query: 1   MACTSVAIGNSPAAAMTKDIIFKEYNGLRRSHSSKD-VRERACIKRSYSDNQIC----YS 60
           MA  +     SPAAA   + + +E+ GLRRS S +D V +   I+RS SDN +C     +
Sbjct: 1   MAFNTAMASTSPAAA---NDVLREHIGLRRSLSGQDLVLKGGGIRRSSSDNHLCCRSGNN 60

Query: 61  VNKIHATSTQPKPKNNNNSSMGMGIFPLKFSGSFLPNAVRSFLFDMEETSKDLSSEEEV- 120
            N+I A S +P  K    +S  +G+F  + S S +P+ +++ LF+  +TS+D    +E+ 
Sbjct: 61  NNRILAVSVRPGMK----TSRSVGVFSFQISSSIIPSPIKTLLFE-TDTSQDEQESDEIE 120

Query: 121 ---------TKRANWIERLLEIRSRWRKKQEKGVENDLYADHDESAESLCG-GDDGGCEV 180
                     K+ANW+ERLLEIR +W+++Q+    N   A  +ES +  CG  ++ GC  
Sbjct: 121 IETEPNLDGAKKANWVERLLEIRRQWKREQKTESGNSDVA--EESVDVTCGCEEEEGCIA 180

Query: 181 DYYDSEDEEGLTFDTESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGL 240
           +Y     + G     ESFSR L++V  S+ K  SQLA+L N+AY I +IK EDL   +GL
Sbjct: 181 NYGSVNGDWG----RESFSRLLVKVSWSEAKKLSQLAYLCNLAYTIPEIKGEDLRRNYGL 240

Query: 241 QFVTSSLRKKADAAAINIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYE 300
           +FVTSSL KKA AA   ++EKL+Q  T +         S  +E+ +  +     +A AY+
Sbjct: 241 KFVTSSLEKKAKAAI--LREKLEQDPTHVP-----VITSPDLESEKQSQRSASSSASAYK 300

Query: 301 IAATAASYVHSRAKNPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQE 360
           IAA+AASY+HS  +   S P+            Y    AA  AASTMTAVVAA E +K E
Sbjct: 301 IAASAASYIHSCKEYDLSEPI------------YKSAAAAQAAASTMTAVVAAGEEEKLE 360

Query: 361 TAKDLQSLHSSPCEWF---------------GSDSLASWQANLFFEPTKFEGTDVLVHRG 420
            A++LQSL SSPCEWF               GSDSLASW+ANLFFEPTKFE TDVLVHRG
Sbjct: 361 AARELQSLQSSPCEWFVCDDPNTYTRCFVIQGSDSLASWKANLFFEPTKFEDTDVLVHRG 420

Query: 421 IYEAAKGIYKQFMPEIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQ 480
           IYEAAKGIY+QF+PEI +HL ++G  AKFQFTGHSLGGSLSL+V+LML+  G+V    ++
Sbjct: 421 IYEAAKGIYEQFLPEITEHLSRHGDRAKFQFTGHSLGGSLSLIVNLMLISRGLVSSEAMK 480

Query: 481 PVVTFGSPFVFCGGHKILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGS 540
            VVTFGSPFVFCGG KIL ELGLDE  ++C++MHRDIVPRAFSCNYP+HVA VLKRL+GS
Sbjct: 481 SVVTFGSPFVFCGGEKILAELGLDESHVHCVMMHRDIVPRAFSCNYPDHVALVLKRLNGS 540

Query: 541 FRSHSCLNKSKLLYTPLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRAF 600
           FR+H CLNK+KLLY+P+GK++ILQP E  SP HP LP G+ALY L+++  GYS   LRAF
Sbjct: 541 FRTHPCLNKNKLLYSPMGKVYILQPSESVSPTHPWLPPGNALYILENSNEGYSPTALRAF 600

Query: 601 LNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLKQHTKMNVEKVRKQRKLLWPL 660
           LN PHPLETLS   AYGSEG++LRDHDS  Y+KA+NGVL+QHTK+ V K R QR+ +WP+
Sbjct: 601 LNRPHPLETLSQRAAYGSEGSVLRDHDSKNYVKAVNGVLRQHTKLIVRKARIQRRSVWPV 649

BLAST of Lsi02G020250 vs. TAIR 10
Match: AT1G02660.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 450.3 bits (1157), Expect = 2.7e-126
Identity = 276/635 (43.46%), Postives = 376/635 (59.21%), Query Frame = 0

Query: 53  CYSVNKIHATSTQPKPKNNN-------------NSSMGMGIFPLKFSGSFLPNAVRSFLF 112
           C  V ++ AT++ P  K                +   G GI   + SG  L +AV   L 
Sbjct: 25  CGGVVEVRATASAPSQKRGPFGFSFKYPLTPFWSRGGGGGIASRRRSGLCLDDAV---LV 84

Query: 113 DMEETSKDLSSEEEV---TKRAN--WIERLLEIRSRWRKKQEKGVE--NDLYADHDESAE 172
           D  ++ K ++ E  V   T+R N  W+ ++L+++S W+ ++E+  +   D   D DE  E
Sbjct: 85  DSGDSRKPIAEETAVEMDTERRNGSWVLKILDVQSTWKHEEEEDDDEVEDEDGDEDEEVE 144

Query: 173 ---SLCGGDDGGCEVDYY---DSEDEEGLTFDTESFSRFLIRVPLSDTKVFSQLAFLSNM 232
              ++   DDGGC+V      D  +      D ESFS+ L RV L ++K+++QL++L N+
Sbjct: 145 LDDAVVSEDDGGCDVCSVLEDDGNEANKFQLDRESFSKLLRRVTLPESKLYAQLSYLGNL 204

Query: 233 AYVIQKIKAEDLEIYHGLQFVTSSLRKKADAAAINIKEKLDQHSTCISDEAFNAGESYSV 292
           AY I KIK  +L  Y+GL+FVTSS  K   A      E   +    +  E         V
Sbjct: 205 AYSISKIKPANLSKYYGLRFVTSSAEKTESALKAENGEVSGETKPIVEAEE-------EV 264

Query: 293 EALELERTRQIPTAVAYEIAATAASYVHSRAKNPSSHPLESQEKGESSTR----AYNPEV 352
           E  E  ++R+I  + AYEI A+AASY+HSR  N    P  S  K E+S +      N E 
Sbjct: 265 EEEEKNKSRKISASAAYEIVASAASYLHSRTNN--ILPFNSSSKAENSDKHDVNLTNAES 324

Query: 353 AAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEWF---------------GSDSLAS 412
           ++ VA S +T+VVAAEE  KQ  A DL+S  SSPC+WF               GS+SLAS
Sbjct: 325 SSDVAYS-VTSVVAAEEDVKQAVADDLKSTISSPCDWFICDDDQSHTRFVVIQGSESLAS 384

Query: 413 WQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMPEIIDHLKKYGTHAKFQFTGHSLGG 472
           WQANL FEP +FEG   +VHRGIYEAAKG+Y+Q +PE+  H+K +GT AKF+FTGHSLGG
Sbjct: 385 WQANLLFEPIEFEGLGAIVHRGIYEAAKGMYEQMLPEVKAHIKTHGTSAKFRFTGHSLGG 444

Query: 473 SLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGGHKILNELGLDEDDIYCIIMHRDIV 532
           SLSLL++LMLL  G V  + L PV+T+G+PFV CGG ++L +LGL +  +  I+MHRDIV
Sbjct: 445 SLSLLLNLMLLVRGEVPASSLLPVITYGAPFVLCGGDRLLKKLGLPKSHVQAIVMHRDIV 504

Query: 533 PRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYTPLGKLFILQPDEMASPPHPMLPL 592
           PRAFSCNYP HVA +LK ++G+FRSH CLNK  +LY+P+G+L ILQPDE  SP H +LP 
Sbjct: 505 PRAFSCNYPYHVAELLKAVNGNFRSHPCLNKQSMLYSPMGELLILQPDETFSPGHELLPS 564

Query: 593 GSALYTLDS---------TQNGYSKGLLRAFLNCPHPLETLSDPTAYGSEGTILRDHDSS 633
           G+ LY L S         +     +     FLN PHPL+ LSD +AYGS GTI RDHD +
Sbjct: 565 GNGLYLLTSDFESPDIEDSDEERLRAAQTVFLNTPHPLDILSDRSAYGSSGTIQRDHDMN 624

BLAST of Lsi02G020250 vs. TAIR 10
Match: AT3G62590.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 405.6 bits (1041), Expect = 7.6e-113
Identity = 249/534 (46.63%), Postives = 321/534 (60.11%), Query Frame = 0

Query: 119 NWIERLLEIRSRWR-KKQEKGVENDLYADHDESA------ESLCGGDDGGCEVDYYDSED 178
           NW+ ++LE+ S W+ K+Q  G       D +E        E LC   D  C +D  D ++
Sbjct: 90  NWVLKILEVGSIWKGKRQRSGGGGGGEEDEEEEVAEPKKKEDLCEECD-FCRIDDDDEDE 149

Query: 179 EEGLTFDTESFSRFLIRVPLSDTKVFSQLAFLSNMAYVIQKIKAEDLEIYHGLQFVTSSL 238
           E+  T     FS  L ++P+ D ++F++L+FL N+AY I KIK E+L  Y  L+FVTSS+
Sbjct: 150 EKEKT--VFEFSEMLSKIPVEDAQMFAKLSFLGNLAYSIPKIKPENLLKYQKLRFVTSSI 209

Query: 239 RKKADAAAINIKEKLDQHSTCISDEAFNAGESYSVEALELERTRQIPTAVAYEIAATAAS 298
            K+       +  K+++++    DE               E+ + I  AVAY IAA+AAS
Sbjct: 210 EKR-------MSLKVEENNNGEEDE---------------EKKKLINPAVAYRIAASAAS 269

Query: 299 YVHSRAKNPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQS 358
            + S +K        S     SS R  N E +    A ++TAVVAA+E  KQ  A DL+S
Sbjct: 270 RLFSHSK--------SVLPFGSSKRQDNEEASLLATADSVTAVVAAKEEVKQAVADDLKS 329

Query: 359 LHSSPCEWF---------------GSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKG 418
             S PCEWF               GSDSLASWQANL FEP  FE  DVLVHRGIYEAAKG
Sbjct: 330 NRSPPCEWFVCDDDKSGTRFFFIQGSDSLASWQANLLFEPVPFEDLDVLVHRGIYEAAKG 389

Query: 419 IYKQFMPEIIDHLKKYGTHAKF-QFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFG 478
           IY+Q +PE+  HL   G +  F +F+GHSLGGSLSLLV+LMLL  G V  + L PV+TFG
Sbjct: 390 IYEQMLPEVHAHLNSRGKNRAFLRFSGHSLGGSLSLLVNLMLLIRGQVPASSLLPVITFG 449

Query: 479 SPFVFCGGHKILNELGLDEDDIYCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSC 538
           SP + CGG ++L +LGL +  +  I MHRDIVPRAFSCNYPN  A +LK L+G+FR+H C
Sbjct: 450 SPCIMCGGDRLLQKLGLPKSHLLGISMHRDIVPRAFSCNYPNRAAKLLKALNGNFRNHPC 509

Query: 539 LNKSKLLYTPLGKLFILQPDEMASPPHPMLPLGSALYTLDSTQNGYSKGLLRA----FLN 598
           LN   +LY+P+GKL ILQP E  SPPHP+LP GS LY L S     ++  LRA    F N
Sbjct: 510 LNNQNVLYSPMGKLLILQPSERFSPPHPLLPPGSGLYLLASKNTDETEKSLRAAKILFFN 569

Query: 599 CPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLKQHTK-MNVEKVRKQRK 625
            PHPLE LSD  +YGSEG I R+HD S YLKAL  V+++  K M  E+ +  RK
Sbjct: 570 SPHPLEILSDRRSYGSEGKIKRNHDMSSYLKALRHVIRKELKQMKAERDQWLRK 590

BLAST of Lsi02G020250 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 2.6e-04
Identity = 39/137 (28.47%), Postives = 62/137 (45.26%), Query Frame = 0

Query: 363 DSLASWQANLFFEPTKFEGTDV-----LVHRGIYEAAKGIYKQFMPEIIDHLKKYGTHAK 422
           D   ++  NLF + +  E         +V R  Y A + I K+ + E          +A+
Sbjct: 281 DDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRLLSE--------HENAR 340

Query: 423 FQFTGHSLGGSLSLLVHLMLLKNGIVK-PTMLQPVVTFGSPFVFCGGHKI----LNELGL 482
           F  TGHSLGG+L++L   +L+ N   +    L  V TFG P +  G  ++      +L  
Sbjct: 341 FVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRI--GNREVGLFMKAQLNQ 400

Query: 483 DEDDIYCIIMHRDIVPR 490
             D  + ++   DIVPR
Sbjct: 401 PVDRYFRVVYCNDIVPR 407

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7Y2203.5e-17150.72Phospholipase A1 PLIP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP1 P... [more]
F4HXL03.8e-12543.46Phospholipase A1 PLIP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP2 P... [more]
Q940L41.1e-11146.63Phospholipase A1 PLIP3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP3 P... [more]
Match NameE-valueIdentityDescription
A0A0A0K4E80.0e+0091.11Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G325710 PE... [more]
A0A1S4E3860.0e+0090.67uncharacterized protein LOC103500094 OS=Cucumis melo OX=3656 GN=LOC103500094 PE=... [more]
A0A5D3BZ410.0e+0090.67Lipase_3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1JSU10.0e+0084.91uncharacterized protein LOC111488580 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JUY60.0e+0084.91uncharacterized protein LOC111488580 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
XP_004139735.10.0e+0091.11phospholipase A1 PLIP1, chloroplastic [Cucumis sativus] >KGN44827.2 hypothetical... [more]
XP_038897374.10.0e+0091.12phospholipase A1 PLIP1, chloroplastic [Benincasa hispida][more]
XP_008461523.10.0e+0090.67PREDICTED: uncharacterized protein LOC103500094 [Cucumis melo] >XP_016902691.1 P... [more]
KAA0051002.10.0e+0090.67uncharacterized protein E6C27_scaffold2606G00220 [Cucumis melo var. makuwa] >TYK... [more]
XP_023547682.10.0e+0085.50uncharacterized protein LOC111806547 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G61680.12.5e-17250.72alpha/beta-Hydrolases superfamily protein [more]
AT1G02660.12.7e-12643.46alpha/beta-Hydrolases superfamily protein [more]
AT3G62590.17.6e-11346.63alpha/beta-Hydrolases superfamily protein [more]
AT3G14360.12.6e-0428.47alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 358..520
e-value: 5.8E-24
score: 86.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 361..532
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 360..494
e-value: 4.5E-24
score: 84.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 296..315
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 296..316
NoneNo IPR availablePANTHERPTHR46483:SF1PHOSPHOLIPASE A1 PLIP1, CHLOROPLASTICcoord: 1..659
NoneNo IPR availableCDDcd00519Lipase_3coord: 361..496
e-value: 8.92926E-23
score: 95.621
IPR043367Phospholipase A1 PLIP1/2/3, chloroplasticPANTHERPTHR46483PHOSPHOLIPASE A1 PLIP2, CHLOROPLASTICcoord: 1..659

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G020250.1Lsi02G020250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015908 fatty acid transport
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0009534 chloroplast thylakoid
molecular_function GO:0052739 phosphatidylserine 1-acylhydrolase activity
molecular_function GO:0008970 phospholipase A1 activity