Lsi02G020100 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G020100
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Auxin efflux carrier component
Location	chr02: 26062275 .. 26064031 (-)
RNA-Seq Expression	Lsi02G020100
Synteny	Lsi02G020100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAACAGTGGAAGATTTCTACAAGGTGATGTGTGCGATGGTTCCACTGTACTTCGCAATGCTGGTGGCTTACAGTTCTGTGAAGTGGTGGAAGATTTTCACCGCGGAGCAATGCGCCGGAATCAACCGTTTCGTGGCGGTGTTCGCGGTGCCGATATTATCCTTCCACTTCATTTCTCAGAACAACCCTTTTCAGATGGACACCAAATTTATATTGGCTGATACTTTCTCTAAGGTTTTTGTTTTGCTTTTGCTCTCTTTTTGGGCTGTCTTCTTCTCCAGCGACCGCGGCGGCAGGCGGCTCGATTGGGTTATCACTCTCTTTTCTTTGGCTACTTTGCCTAACACTTTGGTGATGGGGATTCCTCTTCTCAATGCCATGTATGGTGATTTCACCCAGCCCCTCATGGTCCAACTTGTTGTTCTTCAATGCATCATTTGGTATATCATTTTTATCTTTCCTTCCTCCTTAATATCCATTCATTATTGTTTTTGTTTTTCTCGAGATTCGTACCATTGGAACGATGTGCCCGATGATCTTACATTGTTCGTTATTTAAGATAATTTTGAATTTTTTATCTAAACATACCTTTGTCCTAATTGTCAACAAAAAAAAAAAAAGTTAGACCCAGTTTAATAATTTTTTTGAATTATTTAGAGAATTAAAACAATGTGACTAACAATTTTATATAATTAATGAAAAAGTAGCATTAAACTTTTCTTTCACTACAAGAAATTTAATTTCTTCTAATGTTCAATACCTTGTCAGGATAGGTATGTTCTTTTGACGCTATATTGATGGAGAAGGAAAAAAATCCAAAGTTGAGTTCAAAATTTTAAAACATTATGTGTGTAAAAAGTTTGAACCACAATTTTGATTTTTTTTTTTTTTTTTTTTTAATGAAACTTGAAGAAGAATTTGGATCTATTGGTTGGAAAACAAAGTTTGTGGGCCTATTGTTTGTTTAGACTTTTTTTTTTTCCTTTCAAGATTACTAAACTGGATTTTTCATTAATTGATTTAGGTGTTTGTACTTTTAAGGTAGCAAAAATATAAGTTTTGGTATAATATATACACTCGTGTGATGTGTGCGTAATTATTATACTATGTACAATGGGCATCCATGATTCAATGTTACAAAAGTTAAAATGAAGGATTTTAATATAAAGTTTGACATCTTATTTTGTAGGTATACATTATTGCTCGTTCTCTTTGAATATAGAGCTGCAACTCTATTGATACAAAATCAATTCTCTGGCCCTACCGCTGCTGCCATAGCTAAATTCGAAGTCGATGCTGATGTGATTTCTCTCGACGGTCGAGAGAAACTACTAACCGAGTCAGAGATCGACACACAAGGTGGAATTCGAGTACGAATTCGGCGTTCGACCTCATCGGCACCTGACTCTAGCTTGTCGTCCATCGGCATTACTCCAAGAGCCTCAAATTTCTCCAATGTTGAAATCTTCTCCATCAATACCCCGATGCAACTCCACGGGCCACACCGATCCACCGACATGTCATTTGATTTGGGATCAGGGTATGGGTCATCAGATGCATACTCACTACAACCGACACCGCGGGCATCGAATTTCAACGAGATGGACACAACGGGGAACACACCGACATGGGGAAGGTCCCCGGTGGGAGGGAGGATATTTCGGCAGGGGAGTCCGACGGTGAGGATGGTGTGGGAGTCGCCGGAGAATGGAGGAGAGGGACAAGGATACAAAGCTGTATTGCCAGGTACGTAG

mRNA sequence

ATGATAACAGTGGAAGATTTCTACAAGGTGATGTGTGCGATGGTTCCACTGTACTTCGCAATGCTGGTGGCTTACAGTTCTGTGAAGTGGTGGAAGATTTTCACCGCGGAGCAATGCGCCGGAATCAACCGTTTCGTGGCGGTGTTCGCGGTGCCGATATTATCCTTCCACTTCATTTCTCAGAACAACCCTTTTCAGATGGACACCAAATTTATATTGGCTGATACTTTCTCTAAGGTTTTTGTTTTGCTTTTGCTCTCTTTTTGGGCTGTCTTCTTCTCCAGCGACCGCGGCGGCAGGCGGCTCGATTGGGTTATCACTCTCTTTTCTTTGGCTACTTTGCCTAACACTTTGGTGATGGGGATTCCTCTTCTCAATGCCATGTATGGTGATTTCACCCAGCCCCTCATGGTCCAACTTGTTGTTCTTCAATGCATCATTTGGTATACATTATTGCTCGTTCTCTTTGAATATAGAGCTGCAACTCTATTGATACAAAATCAATTCTCTGGCCCTACCGCTGCTGCCATAGCTAAATTCGAAGTCGATGCTGATGTGATTTCTCTCGACGGTCGAGAGAAACTACTAACCGAGTCAGAGATCGACACACAAGGTGGAATTCGAGTACGAATTCGGCGTTCGACCTCATCGGCACCTGACTCTAGCTTGTCGTCCATCGGCATTACTCCAAGAGCCTCAAATTTCTCCAATGTTGAAATCTTCTCCATCAATACCCCGATGCAACTCCACGGGCCACACCGATCCACCGACATGTCATTTGATTTGGGATCAGGGTATGGGTCATCAGATGCATACTCACTACAACCGACACCGCGGGCATCGAATTTCAACGAGATGGACACAACGGGGAACACACCGACATGGGGAAGGTCCCCGGTGGGAGGGAGGATATTTCGGCAGGGGAGTCCGACGGTGAGGATGGTGTGGGAGTCGCCGGAGAATGGAGGAGAGGGACAAGGATACAAAGCTGTATTGCCAGGTACGTAG

Coding sequence (CDS)

ATGATAACAGTGGAAGATTTCTACAAGGTGATGTGTGCGATGGTTCCACTGTACTTCGCAATGCTGGTGGCTTACAGTTCTGTGAAGTGGTGGAAGATTTTCACCGCGGAGCAATGCGCCGGAATCAACCGTTTCGTGGCGGTGTTCGCGGTGCCGATATTATCCTTCCACTTCATTTCTCAGAACAACCCTTTTCAGATGGACACCAAATTTATATTGGCTGATACTTTCTCTAAGGTTTTTGTTTTGCTTTTGCTCTCTTTTTGGGCTGTCTTCTTCTCCAGCGACCGCGGCGGCAGGCGGCTCGATTGGGTTATCACTCTCTTTTCTTTGGCTACTTTGCCTAACACTTTGGTGATGGGGATTCCTCTTCTCAATGCCATGTATGGTGATTTCACCCAGCCCCTCATGGTCCAACTTGTTGTTCTTCAATGCATCATTTGGTATACATTATTGCTCGTTCTCTTTGAATATAGAGCTGCAACTCTATTGATACAAAATCAATTCTCTGGCCCTACCGCTGCTGCCATAGCTAAATTCGAAGTCGATGCTGATGTGATTTCTCTCGACGGTCGAGAGAAACTACTAACCGAGTCAGAGATCGACACACAAGGTGGAATTCGAGTACGAATTCGGCGTTCGACCTCATCGGCACCTGACTCTAGCTTGTCGTCCATCGGCATTACTCCAAGAGCCTCAAATTTCTCCAATGTTGAAATCTTCTCCATCAATACCCCGATGCAACTCCACGGGCCACACCGATCCACCGACATGTCATTTGATTTGGGATCAGGGTATGGGTCATCAGATGCATACTCACTACAACCGACACCGCGGGCATCGAATTTCAACGAGATGGACACAACGGGGAACACACCGACATGGGGAAGGTCCCCGGTGGGAGGGAGGATATTTCGGCAGGGGAGTCCGACGGTGAGGATGGTGTGGGAGTCGCCGGAGAATGGAGGAGAGGGACAAGGATACAAAGCTGTATTGCCAGGTACGTAG

Protein sequence

MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFISQNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVMGIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKFEVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEIFSINTPMQLHGPHRSTDMSFDLGSGYGSSDAYSLQPTPRASNFNEMDTTGNTPTWGRSPVGGRIFRQGSPTVRMVWESPENGGEGQGYKAVLPGT

Homology

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BLAST of Lsi02G020100 vs. ExPASy Swiss-Prot
Match: Q9SQH6 (Auxin efflux carrier component 6 OS=Arabidopsis thaliana OX=3702 GN=PIN6 PE=2 SV=2)

HSP 1 Score: 400.6 bits (1028), Expect = 1.8e-110
Identity = 227/350 (64.86%), Postives = 256/350 (73.14%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT  +FY VMCAM PLYFAM VAY SVKW KIFT  QC+GINRFV+VFAVP+LSFHFIS
Sbjct: 1   MITGNEFYTVMCAMAPLYFAMFVAYGSVKWCKIFTPAQCSGINRFVSVFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNP++MDT FILADT SK+FV +LLS WAVFF +  GG  LDW+ITLFS+ATLPNTLVM
Sbjct: 61  QNNPYKMDTMFILADTLSKIFVFVLLSLWAVFFKA--GG--LDWLITLFSIATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL AMYGD+TQ LMVQLVVLQCIIWYTLLL LFE RAA LLI+ +F G  A +IAK 
Sbjct: 121 GIPLLQAMYGDYTQTLMVQLVVLQCIIWYTLLLFLFELRAARLLIRAEFPGQAAGSIAKI 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSL-SSIGITPRASNFSNVE 240
           +VD DVISLDG + L TE+E D  G IR+RIRRS SS PDS + SS+ +TPRASN SN E
Sbjct: 181 QVDDDVISLDGMDPLRTETETDVNGRIRLRIRRSVSSVPDSVMSSSLCLTPRASNLSNAE 240

Query: 241 IFSINTPMQ--LHGPHRSTDMSF------------DLG----------------SGYGSS 300
           IFS+NTP     HG   S  + F            DLG                SGY SS
Sbjct: 241 IFSVNTPNNRFFHGGGGSGTLQFYNGSNEIMFCNGDLGGFGFTRPGLGASPRRLSGYASS 300

Query: 301 DAYSLQPTPRASNFNEMDTTGN-TPTWGRSPVGGRIFRQGSPTVRMVWES 319
           DAYSLQPTPRASNFNE+D  GN TP W +SP  GRI+RQ SP  +M+WES
Sbjct: 301 DAYSLQPTPRASNFNELDVNGNGTPVWMKSPAAGRIYRQSSP--KMMWES 344

[Close]

BLAST of Lsi02G020100 vs. ExPASy Swiss-Prot
Match: Q9C6B8 (Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.4e-75
Identity = 172/348 (49.43%), Postives = 209/348 (60.06%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT  DFY VM AMVPLY AM++AY SVKWWKIFT +QC+GINRFVA+FAVP+LSFHFI+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
            NNP+ M+ +F+ AD+  KV VL LL  W       R G  LDW ITLFSL+TLPNTLVM
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWC---KLSRNG-SLDWTITLFSLSTLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL  MYG+F+  LMVQ+VVLQCIIWYTL+L LFEYR A LLI  QF   TA +I   
Sbjct: 121 GIPLLKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFP-DTAGSIVSI 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPD---SSLSSIGITPRASNFSN 240
            VD+D++SLDGR+ L TE+EI   G + V +RRS +S  D        +  TPR SN +N
Sbjct: 181 HVDSDIMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSATPRPSNLTN 240

Query: 241 VEIFSINT---PMQLHGPHRSTDMSFDLGSGYGSSDAY--------SLQPTPRASNFNEM 300
            EI+S+ +   P         TD    + SG G +  +        S  PTPR SN+ E 
Sbjct: 241 AEIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEED 300

Query: 301 DTTGNTPTWGRSPVGGRIFRQGSPTVRMVWESPENGGEGQGYKAVLPG 335
                    G +   GR   Q   +          GG G  Y A  PG
Sbjct: 301 GGPAKPTAAGTAAGAGRFHYQSGGS---------GGGGGAHYPAPNPG 334

[Close]

BLAST of Lsi02G020100 vs. ExPASy Swiss-Prot
Match: Q67UL3 (Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1C PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.5e-74
Identity = 163/297 (54.88%), Postives = 203/297 (68.35%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT  DFY VM AMVPLY AM++AY SVKWW+IFT +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITGADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
            NNP+ M+ +FI ADT  K+ VL LL+ W+    S RG   L+W ITLFSL+TLPNTLVM
Sbjct: 61  TNNPYTMNLRFIAADTLQKLIVLALLTLWS--HLSRRGS--LEWTITLFSLSTLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL  MYG+F+  LMVQ+VVLQCIIWYTL+L +FEYR A +LI  QF   TA AIA  
Sbjct: 121 GIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARILITEQFP-DTAGAIASI 180

Query: 181 EVDADVISLDGREKLL-TESEIDTQGGIRVRIRRSTSSAPD----SSLSSIGITPRASNF 240
            VDADV+SLDGR  ++ TE+E+   G I V +RRS +S  D     S+     TPR SN 
Sbjct: 181 VVDADVVSLDGRRDMIETEAEVKEDGKIHVTVRRSNASRSDVYSRRSMGFSSTTPRPSNL 240

Query: 241 SNVEIFSINT---PMQLHGPHRSTDMSFDLG--SGYGSSDAYSLQ--PTPRASNFNE 286
           +N EI+S+ +   P         TD    +G  S + + DA+ ++   TPR SN+ E
Sbjct: 241 TNAEIYSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFAAGDAFGVRTGATPRPSNYEE 292

[Close]

BLAST of Lsi02G020100 vs. ExPASy Swiss-Prot
Match: Q9S7Z8 (Auxin efflux carrier component 3 OS=Arabidopsis thaliana OX=3702 GN=PIN3 PE=1 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.5e-74
Identity = 162/311 (52.09%), Postives = 212/311 (68.17%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MI+  D Y V+ A++PLY AM++AY SV+WWKIF+ +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MISWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
            NNP+ M+ +FI ADT  K+ +L LL  WA F  S      L+W IT+FSL+TLPNTLVM
Sbjct: 61  TNNPYAMNLRFIAADTLQKIIMLSLLVLWANFTRSG----SLEWSITIFSLSTLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL AMYG+++  LMVQ+VVLQCIIWYTLLL LFE+R A +LI  QF   TAA+I  F
Sbjct: 121 GIPLLIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEFRGAKMLIMEQFP-ETAASIVSF 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEI 240
           +V++DV+SLDG + L T++EI   G + V +R+S +S    S     +TPR SN +  EI
Sbjct: 181 KVESDVVSLDGHDFLETDAEIGDDGKLHVTVRKSNASR--RSFCGPNMTPRPSNLTGAEI 240

Query: 241 FSIN-TPMQLHGPHRS--TDMSFDLG--SGYGSSDAYSLQ----PTPRASNFNEMDTTGN 300
           +S++ TP   +  H      M F  G  S +G +D YS+Q    PTPR SNF E     +
Sbjct: 241 YSLSTTPRGSNFNHSDFYNMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEENCAMAS 300

Query: 301 TPTWGRSPVGG 303
           +P +G  P GG
Sbjct: 301 SPRFGYYPGGG 304

[Close]

BLAST of Lsi02G020100 vs. ExPASy Swiss-Prot
Match: Q940Y5 (Auxin efflux carrier component 7 OS=Arabidopsis thaliana OX=3702 GN=PIN7 PE=1 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 1.3e-73
Identity = 161/311 (51.77%), Postives = 210/311 (67.52%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT  D Y V+ A++PLY AM++AY SV+WWKIF+ +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
            NNP+ M+ +FI ADT  K+ +L LL  WA F  S      L+W IT+FSL+TLPNTLVM
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSG----SLEWSITIFSLSTLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL AMYG+++  LMVQ+VVLQCIIWYTLLL LFEYR A +LI  QF   T A+I  F
Sbjct: 121 GIPLLIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFP-ETGASIVSF 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPD-SSLSSIGITPRASNFSNVE 240
           +V++DV+SLDG + L T+++I   G + V +R+S +S           +TPR SN +  E
Sbjct: 181 KVESDVVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAE 240

Query: 241 IFSIN-TPMQLHGPHRS--TDMSFDLG--SGYGSSDAYSLQ----PTPRASNFNEMDTTG 300
           I+S+N TP   +  H    + M F  G  S +G +D YS+Q    PTPR SNF E     
Sbjct: 241 IYSLNTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEESCAMA 300

Query: 301 NTPTWGRSPVG 302
           ++P +G  P G
Sbjct: 301 SSPRFGYYPGG 306

[Close]

BLAST of Lsi02G020100 vs. ExPASy TrEMBL
Match: A0A6J1ITZ9 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111480565 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 4.0e-150
Identity = 287/338 (84.91%), Postives = 299/338 (88.46%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT+ DFYKVMCAMVPLYFAMLVAY+SVKWWKIFTAEQCAGINRFVAVFAVP+LSFHFIS
Sbjct: 1   MITLGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNPF+MDTKFILADT SK FVLL LS  AVFFS   GG RLDWVITLFSLATLPNTLVM
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFS---GGGRLDWVITLFSLATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLLNAMYGDFTQPLMVQL+VLQCIIWYTLLL LFEYRAA  LI NQF GP+A AI KF
Sbjct: 121 GIPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKF 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEI 240
           E+DADVISLDGR+KLLTESEID QG IRVRIRRSTSSAPDS LSSIGITPRASN SN EI
Sbjct: 181 ELDADVISLDGRDKLLTESEIDIQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEI 240

Query: 241 FSINTPMQLHGPHRSTDMSFDLGSGYGSSDAYSLQPTPRASNFNEMD---TTGNTPTWGR 300
           FSINTP+QLHGP RS DMSFDLGSGYGSSDAYSLQPTPRASNFN+M+   T GNTPTWGR
Sbjct: 241 FSINTPVQLHGPLRSNDMSFDLGSGYGSSDAYSLQPTPRASNFNDMELTTTAGNTPTWGR 300

Query: 301 SPVGGRIFRQGSPTVRMVWESPEN--GGEGQGYKAVLP 334
           SPV       GS  V+MVWESPEN  GGEGQGYKAVLP
Sbjct: 301 SPVA------GSSAVKMVWESPENGGGGEGQGYKAVLP 329

[Close]

BLAST of Lsi02G020100 vs. ExPASy TrEMBL
Match: A0A6J1EKF9 (Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111433384 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.4e-147
Identity = 283/338 (83.73%), Postives = 295/338 (87.28%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MITV DFYKVMCAMVPLYFAMLVAY+SVKWWKIFTAEQCAGINRFVAVFAVP+LSFHFIS
Sbjct: 1   MITVGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNPF+MDTKFILADT SK FVLL LS  AVFFS   GG RLDWVITLFSLATLPNTLVM
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFSGG-GGGRLDWVITLFSLATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLLNAMYGDFTQPLMVQL+VLQCIIWYTLLL LFEYRAA  LI NQF GP+A AI K 
Sbjct: 121 GIPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKI 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEI 240
           E+DADVISLDGR+KLLTESEID QG I VRIRRSTSSAPDS LSSIGITPRASN SN EI
Sbjct: 181 ELDADVISLDGRDKLLTESEIDIQGRICVRIRRSTSSAPDSGLSSIGITPRASNLSNAEI 240

Query: 241 FSINTPMQLHGPHRSTDMSFDLGSGYGSSDAYSLQPTPRASNFNEMD---TTGNTPTWGR 300
           FSINTP+QLHGP RS DMSFDLGSGYGSSDAYS+QPTPR SNFN+M+   T GNT TWGR
Sbjct: 241 FSINTPVQLHGPLRSNDMSFDLGSGYGSSDAYSVQPTPRGSNFNDMEMTTTAGNTATWGR 300

Query: 301 SPVGGRIFRQGSPTVRMVWESPEN--GGEGQGYKAVLP 334
           SPV       GS  V+MVWESPEN  GGEGQGYKAVLP
Sbjct: 301 SPVA------GSSAVKMVWESPENGGGGEGQGYKAVLP 331

[Close]

BLAST of Lsi02G020100 vs. ExPASy TrEMBL
Match: A0A6J1DJM1 (probable auxin efflux carrier component 1b OS=Momordica charantia OX=3673 GN=LOC111021717 PE=4 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 7.1e-139
Identity = 276/342 (80.70%), Postives = 296/342 (86.55%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT  DFYKVMCAMVPLYFAMLVAY+SVKW KIFTA+QCAGINRFVA FAVP+LSFHFIS
Sbjct: 1   MITGGDFYKVMCAMVPLYFAMLVAYTSVKWCKIFTADQCAGINRFVAAFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNPF+MDTKFI+ADT SK+FVL+L+S     F    G   LDWVITLFSLATLPNTLVM
Sbjct: 61  QNNPFEMDTKFIVADTASKIFVLVLVSLGVAVFPGVGG---LDWVITLFSLATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLLNAMYG FTQ LMVQLVVLQCIIWYTLLL LFEYRAA LLIQNQF GPTAAAI KF
Sbjct: 121 GIPLLNAMYGHFTQSLMVQLVVLQCIIWYTLLLFLFEYRAAMLLIQNQFPGPTAAAITKF 180

Query: 181 EVDADVISLDGREKL-LTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVE 240
           ++DADVISLD R++L LTESEID +G IRVRIRRSTSSAPDSSLSSIGITPRASN SN E
Sbjct: 181 KLDADVISLDDRDQLILTESEIDNEGRIRVRIRRSTSSAPDSSLSSIGITPRASNLSNAE 240

Query: 241 IFSINTPMQLHGPHRSTDMSFDLGSGYGSSDAYSLQPTPRASNFNEMDTT---GNTPTWG 300
           IFSINTP QL+ PH+ T    +L SGY SSD YSLQPTPRASNFNEM+TT   GNTPTWG
Sbjct: 241 IFSINTPTQLNSPHQPT----NLNSGY-SSDMYSLQPTPRASNFNEMETTTTAGNTPTWG 300

Query: 301 RSPVGGRIFRQGSP---TVRMVWESPEN-GGEGQGYKAVLPG 335
           RSP+GGRIFRQGSP   +V+MVWESPEN GGEGQGYKAVLPG
Sbjct: 301 RSPMGGRIFRQGSPAVGSVKMVWESPENGGGEGQGYKAVLPG 334

[Close]

BLAST of Lsi02G020100 vs. ExPASy TrEMBL
Match: A0A5B7B6Z8 (Auxin efflux carrier component (Fragment) OS=Davidia involucrata OX=16924 GN=Din_032862 PE=3 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 8.7e-129
Identity = 261/354 (73.73%), Postives = 284/354 (80.23%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MI+  DFYKVMCAMVPLYFAMLVAY+SVKWWKIFT EQC+GINRFVAVFAVP+LSFHFIS
Sbjct: 9   MISANDFYKVMCAMVPLYFAMLVAYASVKWWKIFTPEQCSGINRFVAVFAVPVLSFHFIS 68

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNP+QMDTKFILADT SKV VL+LLS WA+F    +GG  LDW+ITLFS+ATLPNTLVM
Sbjct: 69  QNNPYQMDTKFILADTLSKVLVLVLLSLWAIF----KGG--LDWLITLFSVATLPNTLVM 128

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL AMYGDFTQ LMVQ+VVLQCIIWYTLLL LFEYRAA  LIQNQF GPTAA+I KF
Sbjct: 129 GIPLLKAMYGDFTQSLMVQIVVLQCIIWYTLLLFLFEYRAAATLIQNQFPGPTAASITKF 188

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSL-SSIGITPRASNFSNVE 240
           E+D DVISLDGR+ L TESEID  G IRVRIRRSTSSAPDS+L SSIGITPRASN S  E
Sbjct: 189 EIDGDVISLDGRDPLCTESEIDGNGRIRVRIRRSTSSAPDSALSSSIGITPRASNLSGAE 248

Query: 241 IFSINTPMQLHG-PHRSTDMSF-DLG---------SGYGSSDAYSLQPTPRASNFNEMD- 300
           I+S+NTP   H   H STDM+F DL          SGY SSDAYSLQPTPRASNFNE+D 
Sbjct: 249 IYSVNTPAPPHEFHHASTDMAFGDLAFGYRSSPRLSGYASSDAYSLQPTPRASNFNELDT 308

Query: 301 ---TTGNTPTWGRSPVGGRIFRQGSPT---VRMVWESP----ENGGEGQGYKAV 332
              TT NTP W RSP GG++FRQ SP    ++MVWESP      GGE QG K V
Sbjct: 309 TTVTTANTPIWVRSPAGGKVFRQSSPAFSGMKMVWESPGKCQGGGGERQGCKDV 356

[Close]

BLAST of Lsi02G020100 vs. ExPASy TrEMBL
Match: A0A075IL74 (PIN-like protein (Fragment) OS=Aextoxicon punctatum OX=39268 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 4.8e-127
Identity = 260/357 (72.83%), Postives = 284/357 (79.55%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MI+ EDFYKVMCAMVPLYFAMLVAY SVKWWKIF+ EQC+GINRFVAVFAVP+LSFHFIS
Sbjct: 1   MISAEDFYKVMCAMVPLYFAMLVAYGSVKWWKIFSPEQCSGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNP+QMDTKFILADT SK+FVL+LLS WA    +  GG  LDW+ITLFS+ATLPNTLVM
Sbjct: 61  QNNPYQMDTKFILADTLSKIFVLVLLSLWAXXXFN--GG--LDWLITLFSVATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL AMYGDFTQ LMVQLVVLQCIIWYTLLL LFEYRAATLLIQNQF G TAA+I+KF
Sbjct: 121 GIPLLQAMYGDFTQSLMVQLVVLQCIIWYTLLLFLFEYRAATLLIQNQFPGNTAASISKF 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSL-SSIGITPRASNFSNVE 240
           EVD DVISLDGR+ L TESEID  G IRVRIRRSTSSAPDS+L SSIGITPRASN S  E
Sbjct: 181 EVDGDVISLDGRDPLRTESEIDGTGRIRVRIRRSTSSAPDSALSSSIGITPRASNLSGAE 240

Query: 241 IFSINTPMQLHGPHRS-TDMSFDLG-------------SGYGSSDAYSLQPTPRASNFNE 300
           I+S+NTP Q H  H S TD+ F  G             SGY SSDAYSLQPTPRASNFNE
Sbjct: 241 IYSVNTPAQQHEFHHSNTDIGFGNGDIAFGYRSASPRLSGYASSDAYSLQPTPRASNFNE 300

Query: 301 MD----TTGNTPTWGRSPVGGRIFRQGSPT---VRMVWESP---ENGGEGQGYKAVL 333
           +D    TT  TP W RSP  G+++RQ SP    V+MVWESP   + GGE QG K V+
Sbjct: 301 LDTTTVTTAITPMWVRSPAAGKVYRQQSPVFTGVKMVWESPGKCQGGGERQGCKDVV 353

[Close]

BLAST of Lsi02G020100 vs. NCBI nr
Match: XP_038897269.1 (auxin efflux carrier component 6 [Benincasa hispida])

HSP 1 Score: 579.3 bits (1492), Expect = 2.1e-161
Identity = 299/333 (89.79%), Postives = 308/333 (92.49%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS
Sbjct: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNPF+MDTKFILADT SKVFVLLLLS WAVFFS    G RLDWVITLFSLATLPNTLVM
Sbjct: 61  QNNPFEMDTKFILADTISKVFVLLLLSVWAVFFS----GGRLDWVITLFSLATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLLNAMYGDFTQPLMVQLVVLQCI+WYTLLL LFEYRAATLLIQNQF G TAAAIAK 
Sbjct: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIVWYTLLLFLFEYRAATLLIQNQFPGSTAAAIAKI 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEI 240
           EVDAD+ISLDGR+KLLTE++IDT+G IRVRI RSTSSAP S LSSIGITPRASN SNVEI
Sbjct: 181 EVDADIISLDGRDKLLTETDIDTRGQIRVRICRSTSSAPSSGLSSIGITPRASNLSNVEI 240

Query: 241 FSINTPMQLHGPHRSTDMSFDLGSGYGSSDAYSLQPTPRASNFNEMDTTGNTPTWGRSPV 300
           FSINTP Q HGPHRSTD+SFDLGSGYGSSDAYSLQPTPRASNFNEMD  GNTP WGRSPV
Sbjct: 241 FSINTPTQPHGPHRSTDVSFDLGSGYGSSDAYSLQPTPRASNFNEMDMAGNTPMWGRSPV 300

Query: 301 GGRIFRQGSPTVRMVWESPENGGEGQGYKAVLP 334
           GGRIFRQGSP V+M WESPENG EGQGYKA LP
Sbjct: 301 GGRIFRQGSPVVKMAWESPENGVEGQGYKAELP 329

[Close]

BLAST of Lsi02G020100 vs. NCBI nr
Match: XP_022981447.1 (auxin efflux carrier component 6 [Cucurbita maxima])

HSP 1 Score: 540.8 bits (1392), Expect = 8.3e-150
Identity = 287/338 (84.91%), Postives = 299/338 (88.46%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT+ DFYKVMCAMVPLYFAMLVAY+SVKWWKIFTAEQCAGINRFVAVFAVP+LSFHFIS
Sbjct: 1   MITLGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNPF+MDTKFILADT SK FVLL LS  AVFFS   GG RLDWVITLFSLATLPNTLVM
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFS---GGGRLDWVITLFSLATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLLNAMYGDFTQPLMVQL+VLQCIIWYTLLL LFEYRAA  LI NQF GP+A AI KF
Sbjct: 121 GIPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKF 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEI 240
           E+DADVISLDGR+KLLTESEID QG IRVRIRRSTSSAPDS LSSIGITPRASN SN EI
Sbjct: 181 ELDADVISLDGRDKLLTESEIDIQGRIRVRIRRSTSSAPDSGLSSIGITPRASNLSNAEI 240

Query: 241 FSINTPMQLHGPHRSTDMSFDLGSGYGSSDAYSLQPTPRASNFNEMD---TTGNTPTWGR 300
           FSINTP+QLHGP RS DMSFDLGSGYGSSDAYSLQPTPRASNFN+M+   T GNTPTWGR
Sbjct: 241 FSINTPVQLHGPLRSNDMSFDLGSGYGSSDAYSLQPTPRASNFNDMELTTTAGNTPTWGR 300

Query: 301 SPVGGRIFRQGSPTVRMVWESPEN--GGEGQGYKAVLP 334
           SPV       GS  V+MVWESPEN  GGEGQGYKAVLP
Sbjct: 301 SPVA------GSSAVKMVWESPENGGGGEGQGYKAVLP 329

[Close]

BLAST of Lsi02G020100 vs. NCBI nr
Match: XP_023525387.1 (auxin efflux carrier component 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.8 bits (1379), Expect = 2.7e-148
Identity = 285/338 (84.32%), Postives = 297/338 (87.87%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MITV DFYKVMCAM+PLYFAMLVAY+SVKWWKIFTAEQCAGINRFVAVFAVP+LSFHFIS
Sbjct: 1   MITVGDFYKVMCAMLPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNPF+MDTKFILADT SK FVLL LS  AVFFS   GG RLDWVITLFSLATLPNTLVM
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLSLSLGAVFFSGG-GGGRLDWVITLFSLATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLLNAMYGDFTQPLMVQL+VLQCIIWYTLLL LFEYRAA LLI NQF GP+A AI KF
Sbjct: 121 GIPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAILLIHNQFPGPSAKAITKF 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEI 240
           E+DADVISLDGR+KLLTESEID QG I VRIRRSTSSAPDS LSSIGITPRASN SN EI
Sbjct: 181 ELDADVISLDGRDKLLTESEIDIQGRICVRIRRSTSSAPDSGLSSIGITPRASNLSNAEI 240

Query: 241 FSINTPMQLHGPHRSTDMSFDLGSGYGSSDAYSLQPTPRASNFNEMD---TTGNTPTWGR 300
           FSINTP+QLHGP RS DM FDLGSGYGSSDAYSLQPTPR SNFN+M+   T GNTPTWGR
Sbjct: 241 FSINTPVQLHGPLRSNDMLFDLGSGYGSSDAYSLQPTPRGSNFNDMEMTTTAGNTPTWGR 300

Query: 301 SPVGGRIFRQGSPTVRMVWESPEN--GGEGQGYKAVLP 334
           SPV       GS  V+MVWESPEN  GGEGQGYKAVLP
Sbjct: 301 SPVA------GSSAVKMVWESPENGGGGEGQGYKAVLP 331

[Close]

BLAST of Lsi02G020100 vs. NCBI nr
Match: XP_022926200.1 (auxin efflux carrier component 6 [Cucurbita moschata])

HSP 1 Score: 531.6 bits (1368), Expect = 5.0e-147
Identity = 283/338 (83.73%), Postives = 295/338 (87.28%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MITV DFYKVMCAMVPLYFAMLVAY+SVKWWKIFTAEQCAGINRFVAVFAVP+LSFHFIS
Sbjct: 1   MITVGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNPF+MDTKFILADT SK FVLL LS  AVFFS   GG RLDWVITLFSLATLPNTLVM
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFSGG-GGGRLDWVITLFSLATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLLNAMYGDFTQPLMVQL+VLQCIIWYTLLL LFEYRAA  LI NQF GP+A AI K 
Sbjct: 121 GIPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKI 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEI 240
           E+DADVISLDGR+KLLTESEID QG I VRIRRSTSSAPDS LSSIGITPRASN SN EI
Sbjct: 181 ELDADVISLDGRDKLLTESEIDIQGRICVRIRRSTSSAPDSGLSSIGITPRASNLSNAEI 240

Query: 241 FSINTPMQLHGPHRSTDMSFDLGSGYGSSDAYSLQPTPRASNFNEMD---TTGNTPTWGR 300
           FSINTP+QLHGP RS DMSFDLGSGYGSSDAYS+QPTPR SNFN+M+   T GNT TWGR
Sbjct: 241 FSINTPVQLHGPLRSNDMSFDLGSGYGSSDAYSVQPTPRGSNFNDMEMTTTAGNTATWGR 300

Query: 301 SPVGGRIFRQGSPTVRMVWESPEN--GGEGQGYKAVLP 334
           SPV       GS  V+MVWESPEN  GGEGQGYKAVLP
Sbjct: 301 SPVA------GSSAVKMVWESPENGGGGEGQGYKAVLP 331

[Close]

BLAST of Lsi02G020100 vs. NCBI nr
Match: KAG6607665.1 (Auxin efflux carrier component 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 531.6 bits (1368), Expect = 5.0e-147
Identity = 282/338 (83.43%), Postives = 295/338 (87.28%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT+ DFYKVMCAMVPLYFAMLVAY+SVKWWKIFTAEQCAGINRFVAVFAVP+LSFHFIS
Sbjct: 1   MITLGDFYKVMCAMVPLYFAMLVAYTSVKWWKIFTAEQCAGINRFVAVFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNPF+MDTKFILADT SK FVLL LS  AVFFS   GG RLDWVITLFSLATLPNTLVM
Sbjct: 61  QNNPFEMDTKFILADTISKAFVLLFLSLGAVFFSGG-GGGRLDWVITLFSLATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLLNAMYGDFTQPLMVQL+VLQCIIWYTLLL LFEYRAA  LI NQF GP+A AI K 
Sbjct: 121 GIPLLNAMYGDFTQPLMVQLLVLQCIIWYTLLLFLFEYRAAISLIHNQFPGPSATAITKI 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEI 240
           E+DADVISLDGR+KLLTESEID QG I VRIRRSTSSAPDS  SSIGITPRASN SN EI
Sbjct: 181 ELDADVISLDGRDKLLTESEIDIQGRICVRIRRSTSSAPDSGFSSIGITPRASNLSNAEI 240

Query: 241 FSINTPMQLHGPHRSTDMSFDLGSGYGSSDAYSLQPTPRASNFNEMD---TTGNTPTWGR 300
           FSINTP+QLHGP RS DMSFDLGSGYGSSDAYSLQPTPR SNFN+M+   T GNTPTWGR
Sbjct: 241 FSINTPVQLHGPLRSNDMSFDLGSGYGSSDAYSLQPTPRGSNFNDMEMTTTAGNTPTWGR 300

Query: 301 SPVGGRIFRQGSPTVRMVWESPEN--GGEGQGYKAVLP 334
           SPV       GS  V++VWESPEN  GGEGQGYKAVLP
Sbjct: 301 SPVA------GSSAVKIVWESPENGGGGEGQGYKAVLP 331

[Close]

BLAST of Lsi02G020100 vs. TAIR 10
Match: AT1G77110.1 (Auxin efflux carrier family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.2e-111
Identity = 227/350 (64.86%), Postives = 256/350 (73.14%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT  +FY VMCAM PLYFAM VAY SVKW KIFT  QC+GINRFV+VFAVP+LSFHFIS
Sbjct: 1   MITGNEFYTVMCAMAPLYFAMFVAYGSVKWCKIFTPAQCSGINRFVSVFAVPVLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
           QNNP++MDT FILADT SK+FV +LLS WAVFF +  GG  LDW+ITLFS+ATLPNTLVM
Sbjct: 61  QNNPYKMDTMFILADTLSKIFVFVLLSLWAVFFKA--GG--LDWLITLFSIATLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL AMYGD+TQ LMVQLVVLQCIIWYTLLL LFE RAA LLI+ +F G  A +IAK 
Sbjct: 121 GIPLLQAMYGDYTQTLMVQLVVLQCIIWYTLLLFLFELRAARLLIRAEFPGQAAGSIAKI 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSL-SSIGITPRASNFSNVE 240
           +VD DVISLDG + L TE+E D  G IR+RIRRS SS PDS + SS+ +TPRASN SN E
Sbjct: 181 QVDDDVISLDGMDPLRTETETDVNGRIRLRIRRSVSSVPDSVMSSSLCLTPRASNLSNAE 240

Query: 241 IFSINTPMQ--LHGPHRSTDMSF------------DLG----------------SGYGSS 300
           IFS+NTP     HG   S  + F            DLG                SGY SS
Sbjct: 241 IFSVNTPNNRFFHGGGGSGTLQFYNGSNEIMFCNGDLGGFGFTRPGLGASPRRLSGYASS 300

Query: 301 DAYSLQPTPRASNFNEMDTTGN-TPTWGRSPVGGRIFRQGSPTVRMVWES 319
           DAYSLQPTPRASNFNE+D  GN TP W +SP  GRI+RQ SP  +M+WES
Sbjct: 301 DAYSLQPTPRASNFNELDVNGNGTPVWMKSPAAGRIYRQSSP--KMMWES 344

[Close]

BLAST of Lsi02G020100 vs. TAIR 10
Match: AT1G73590.1 (Auxin efflux carrier family protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 172/348 (49.43%), Postives = 209/348 (60.06%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT  DFY VM AMVPLY AM++AY SVKWWKIFT +QC+GINRFVA+FAVP+LSFHFI+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
            NNP+ M+ +F+ AD+  KV VL LL  W       R G  LDW ITLFSL+TLPNTLVM
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWC---KLSRNG-SLDWTITLFSLSTLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL  MYG+F+  LMVQ+VVLQCIIWYTL+L LFEYR A LLI  QF   TA +I   
Sbjct: 121 GIPLLKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFP-DTAGSIVSI 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPD---SSLSSIGITPRASNFSN 240
            VD+D++SLDGR+ L TE+EI   G + V +RRS +S  D        +  TPR SN +N
Sbjct: 181 HVDSDIMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSATPRPSNLTN 240

Query: 241 VEIFSINT---PMQLHGPHRSTDMSFDLGSGYGSSDAY--------SLQPTPRASNFNEM 300
            EI+S+ +   P         TD    + SG G +  +        S  PTPR SN+ E 
Sbjct: 241 AEIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEED 300

Query: 301 DTTGNTPTWGRSPVGGRIFRQGSPTVRMVWESPENGGEGQGYKAVLPG 335
                    G +   GR   Q   +          GG G  Y A  PG
Sbjct: 301 GGPAKPTAAGTAAGAGRFHYQSGGS---------GGGGGAHYPAPNPG 334

[Close]

BLAST of Lsi02G020100 vs. TAIR 10
Match: AT1G70940.1 (Auxin efflux carrier family protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.5e-75
Identity = 162/311 (52.09%), Postives = 212/311 (68.17%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MI+  D Y V+ A++PLY AM++AY SV+WWKIF+ +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MISWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
            NNP+ M+ +FI ADT  K+ +L LL  WA F  S      L+W IT+FSL+TLPNTLVM
Sbjct: 61  TNNPYAMNLRFIAADTLQKIIMLSLLVLWANFTRSG----SLEWSITIFSLSTLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL AMYG+++  LMVQ+VVLQCIIWYTLLL LFE+R A +LI  QF   TAA+I  F
Sbjct: 121 GIPLLIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEFRGAKMLIMEQFP-ETAASIVSF 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPDSSLSSIGITPRASNFSNVEI 240
           +V++DV+SLDG + L T++EI   G + V +R+S +S    S     +TPR SN +  EI
Sbjct: 181 KVESDVVSLDGHDFLETDAEIGDDGKLHVTVRKSNASR--RSFCGPNMTPRPSNLTGAEI 240

Query: 241 FSIN-TPMQLHGPHRS--TDMSFDLG--SGYGSSDAYSLQ----PTPRASNFNEMDTTGN 300
           +S++ TP   +  H      M F  G  S +G +D YS+Q    PTPR SNF E     +
Sbjct: 241 YSLSTTPRGSNFNHSDFYNMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEENCAMAS 300

Query: 301 TPTWGRSPVGG 303
           +P +G  P GG
Sbjct: 301 SPRFGYYPGGG 304

[Close]

BLAST of Lsi02G020100 vs. TAIR 10
Match: AT1G23080.1 (Auxin efflux carrier family protein )

HSP 1 Score: 278.1 bits (710), Expect = 9.3e-75
Identity = 161/311 (51.77%), Postives = 210/311 (67.52%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT  D Y V+ A++PLY AM++AY SV+WWKIF+ +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
            NNP+ M+ +FI ADT  K+ +L LL  WA F  S      L+W IT+FSL+TLPNTLVM
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSG----SLEWSITIFSLSTLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL AMYG+++  LMVQ+VVLQCIIWYTLLL LFEYR A +LI  QF   T A+I  F
Sbjct: 121 GIPLLIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFP-ETGASIVSF 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPD-SSLSSIGITPRASNFSNVE 240
           +V++DV+SLDG + L T+++I   G + V +R+S +S           +TPR SN +  E
Sbjct: 181 KVESDVVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAE 240

Query: 241 IFSIN-TPMQLHGPHRS--TDMSFDLG--SGYGSSDAYSLQ----PTPRASNFNEMDTTG 300
           I+S+N TP   +  H    + M F  G  S +G +D YS+Q    PTPR SNF E     
Sbjct: 241 IYSLNTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEESCAMA 300

Query: 301 NTPTWGRSPVG 302
           ++P +G  P G
Sbjct: 301 SSPRFGYYPGG 306

[Close]

BLAST of Lsi02G020100 vs. TAIR 10
Match: AT1G23080.2 (Auxin efflux carrier family protein )

HSP 1 Score: 278.1 bits (710), Expect = 9.3e-75
Identity = 161/311 (51.77%), Postives = 210/311 (67.52%), Query Frame = 0


Query: 1   MITVEDFYKVMCAMVPLYFAMLVAYSSVKWWKIFTAEQCAGINRFVAVFAVPILSFHFIS 60
           MIT  D Y V+ A++PLY AM++AY SV+WWKIF+ +QC+GINRFVA+FAVP+LSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  QNNPFQMDTKFILADTFSKVFVLLLLSFWAVFFSSDRGGRRLDWVITLFSLATLPNTLVM 120
            NNP+ M+ +FI ADT  K+ +L LL  WA F  S      L+W IT+FSL+TLPNTLVM
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSG----SLEWSITIFSLSTLPNTLVM 120

Query: 121 GIPLLNAMYGDFTQPLMVQLVVLQCIIWYTLLLVLFEYRAATLLIQNQFSGPTAAAIAKF 180
           GIPLL AMYG+++  LMVQ+VVLQCIIWYTLLL LFEYR A +LI  QF   T A+I  F
Sbjct: 121 GIPLLIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFP-ETGASIVSF 180

Query: 181 EVDADVISLDGREKLLTESEIDTQGGIRVRIRRSTSSAPD-SSLSSIGITPRASNFSNVE 240
           +V++DV+SLDG + L T+++I   G + V +R+S +S           +TPR SN +  E
Sbjct: 181 KVESDVVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAE 240

Query: 241 IFSIN-TPMQLHGPHRS--TDMSFDLG--SGYGSSDAYSLQ----PTPRASNFNEMDTTG 300
           I+S+N TP   +  H    + M F  G  S +G +D YS+Q    PTPR SNF E     
Sbjct: 241 IYSLNTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEESCAMA 300

Query: 301 NTPTWGRSPVG 302
           ++P +G  P G
Sbjct: 301 SSPRFGYYPGG 306

The following BLAST results are available for this feature:

BLAST of Lsi02G020100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SQH6	1.8e-110	64.86	Auxin efflux carrier component 6 OS=Arabidopsis thaliana OX=3702 GN=PIN6 PE=2 SV...	[more]
Q9C6B8	2.4e-75	49.43	Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV...	[more]
Q67UL3	3.5e-74	54.88	Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39...	[more]
Q9S7Z8	3.5e-74	52.09	Auxin efflux carrier component 3 OS=Arabidopsis thaliana OX=3702 GN=PIN3 PE=1 SV...	[more]
Q940Y5	1.3e-73	51.77	Auxin efflux carrier component 7 OS=Arabidopsis thaliana OX=3702 GN=PIN7 PE=1 SV...	[more]

BLAST of Lsi02G020100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ITZ9	4.0e-150	84.91	Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111480565 PE=3 ...	[more]
A0A6J1EKF9	2.4e-147	83.73	Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111433384 PE=...	[more]
A0A6J1DJM1	7.1e-139	80.70	probable auxin efflux carrier component 1b OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A5B7B6Z8	8.7e-129	73.73	Auxin efflux carrier component (Fragment) OS=Davidia involucrata OX=16924 GN=Din...	[more]
A0A075IL74	4.8e-127	72.83	PIN-like protein (Fragment) OS=Aextoxicon punctatum OX=39268 PE=2 SV=1	[more]

BLAST of Lsi02G020100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897269.1	2.1e-161	89.79	auxin efflux carrier component 6 [Benincasa hispida]	[more]
XP_022981447.1	8.3e-150	84.91	auxin efflux carrier component 6 [Cucurbita maxima]	[more]
XP_023525387.1	2.7e-148	84.32	auxin efflux carrier component 6 [Cucurbita pepo subsp. pepo]	[more]
XP_022926200.1	5.0e-147	83.73	auxin efflux carrier component 6 [Cucurbita moschata]	[more]
KAG6607665.1	5.0e-147	83.43	Auxin efflux carrier component 6, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of Lsi02G020100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G77110.1	1.2e-111	64.86	Auxin efflux carrier family protein	[more]
AT1G73590.1	1.7e-76	49.43	Auxin efflux carrier family protein	[more]
AT1G70940.1	2.5e-75	52.09	Auxin efflux carrier family protein	[more]
AT1G23080.1	9.3e-75	51.77	Auxin efflux carrier family protein	[more]
AT1G23080.2	9.3e-75	51.77	Auxin efflux carrier family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004776	Membrane transport protein	PFAM	PF03547	Mem_trans	coord: 10..246 e-value: 2.1E-85 score: 286.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 283..302
None	No IPR available	PANTHER	PTHR31752	AUXIN EFFLUX CARRIER COMPONENT 1B-RELATED	coord: 1..315
None	No IPR available	PANTHER	PTHR31752:SF56	AUXIN EFFLUX CARRIER COMPONENT 6	coord: 1..315

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G020100.1	Lsi02G020100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010315	auxin efflux
biological_process	GO:0010252	auxin homeostasis
biological_process	GO:0009926	auxin polar transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0010329	auxin efflux transmembrane transporter activity