Lsi02G019410 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G019410
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
Locationchr02: 25324493 .. 25328201 (-)
RNA-Seq ExpressionLsi02G019410
SyntenyLsi02G019410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCATCTTCTCAACACCTCCTTCGTCGCCGCCTCCTCCCCATCTCTCCGGCGGAGGCCTTTTCTCCGACACCCATTTCACCCTACACGGCGGTCTCTTCAAGTCCAGGCCAAGATCCGCGAGATCTTCATGCCTGCTCTCAGCTCCACCATGACCGAGGGCAAGATTGTGTCCTGGATCAAGACTGAAGGCGACAAGCTCGCCAAGGGCGAGAGTGTTGTCGTCGTTGAGTCCGATAAGGCCGATATGGACGTCGAGACTTTCTATGATGGCTATCTTGCTGCCATTATGGTTGATGAAGGAGGCGTTGCGCCTGTTGGATCTGCAATCGCGCTTTTGGCTGAGACTGAGGATGAGATTTCTGAGGCTAAGTCTAAAGCTGCCATCTCTTCTGCTTCTCCGGCTTCGGCACCTCCGCCGGAGAAGAGTCCTGACAATGCAGCGGCAGTACCGGTTGCAGCGGCGGCACCAGTTTCGGTGGCGAAGGCGGCGAAGGTGGCGAAGGTGGTAGTGGCTTCCACTCACCCTGCATCTGAAGGGGGGAAGAGAATTGTGGCGTCTCCGTATGCGAAGAAGCTGGCTAAGGAGTTGAATGTGGAGTTGGCGACGGTGGTGGGGACTGGGCCATTGGGGAGAATTGTGGCTAAGGATGTTGAAGCTGCGGCTTCTGCTGCTGCTAGCTCAGTTTCGGCTCCGGGAGGCGGTGTGAAGCCCGCCCCATCCCTGGAGTTGGGAACCACTGTGCCGTTTACCACAATGCAAGGGGCGGTGAGTAGGAATATGGTTGAGAGTCTCGCGGTGCCCACTTTCAGAGTGGGGTATACCATTACAACAGATGCGCTTGATTCTCTTTACAAAAAGGTAAATTCATCGATTTGGAGACTATCATTGATTCATTGCCTTTTTCCGTTCTATGAAACTGGCTTTCAAACTTATGTTGGACATGTTATTAGTTAGAATTACTTCATGATTTGAAAAATTGAAACGACTTTCTTTTCGAAAGGATGGAATTGGAAGTTGTCTTCTGCTCTGCTAATAATTGGAAGTGATTTATGTTTCTTAGATTAAATCTAAGGGAGTGACCATGACAGCGTTGCTTGCCAAGGCTACAGCTCTAGCATTGGCTAAACATCCGGTAGTAAATTCAAGTTGTAGGGATGGTAAGAGCTTTACTTATAATAGAAGTATCAATATTGCAGTTGCAGTTGCCATTGATGGTGGCTTGATCACACCAGTGCTTCAAGATGCGGATAAGGTTATATAAGTTTCCTTTAAGTTGTTCTGCTATTTTTCAAATGATGTGTTAATTATTTAATCTTGGATTGTTTATACTTGATTTGTGCTTTAGGTGGACATTTATTCTCTGTCTCGAAAGTGGAAGGAGTTAGTTGATAAGGCTAGAGCCAAGCAGCTGCAACCTCAGGAATACAACACTGGTACTTTTCATTAGTTTATATTTCAACCACATTTCAACAAGGAGTAATTAGTAGTTCACTTGCATTCATATCTACGGAGAAAGTTACTTTATTTTATTCAATTTTTTGAGAAGAGGACTCCCAATGCACCCAAAAGTGAAAGAAGGAAATTCCAAGAAAGCTGAAGAAGAGTTTTGTAAAATAAAGCTTGTCAAAGGAAGAGGAACTCAACTTTGTATAGTCAAGACAACTTGAGGAAATGAATAAATCAAAATGGTAAAGAAGGATAATGGATTACCATTTCTTTTATATCGCAAGGTGTGATGTGACACGTGGAGTGCACATCACGAAAGGCTTGAACGTTTCATTTGTTAATTTTCTTAAAGTGCAATTCTTCTCCTAAGTGCACTCTGAGGTACTCAAGTATGTGGTAGATTAATTTTTTATGTTCTTTTGTATTGTTCATGAGTTGACTAATTTAATGATGGGAGTAGAAAGTCGTCAGCTTATTTTACATATTCTAAGAAGACTGAGCGTGAGTCTGAGTAGGGGAAATGGAGTGATTCTATCTATTGGGGTGGGGGGTGGGGGGTGCAGGGCAAGTGAAAAAGTGATGTACACTGAGAGTGTAAATGTGTGGACTTATGGTTAGTGAGTGGGCTCTCTCTCCTTTAATACAGTTGAGGCTCTAAGGGTAGGAAAGGGAGAAGAGGGGAGTAGCTAGAGAGGCCTTTGTGGAGACTTGAGAGATTTAAACCTCATGAAGTCTGAAGCTTTTCTGAATGCCTTCTCCTTTCCATATTTAATATAGTTCAGTGAAAGAGCCCTAGCACATTCATGCTACTAGTGTTGACCATCTCATTTTGGGAAAACCATTGAAACATCCTAAGAATGCCTCCTTTTAGGTTGTTTCAACTCTTTCTGAAGAATAAAGTAACAATGGCTGTGAAGATTCTGAAATTCTTGAGGTTTATAGCATTAGAGAGGGGGCGGGGGGGAATTTATAGCATTAGACCAATAGCACCTTGGCTTAAGGCGAAGCCACCAGCCAGAATCATGTAGTTGAAAAGATTGCAGATAGATCCAACCCTTGCTTAGCCAAAGAACAGTGGAGAAAAAAGTGATCTTGCGATTCATCACCACATGTGGAAAACCCGAGGAGATATGATGTAGTTGTGCGGATTTATTTTTTGAACTTCATCCAAATTTCTAAGTACTCCACCTTAAGCAAAGGACCATATATAGGAGTTGGTTTTCTTAGGACTAGTCCCTTTCCAATTGATCTTACTAAGATTCTGGTCCCGAAAGTTAGGGCCTTGAGAGAAATAACGGTCAGTCGCTGACTCCCCAGAAAATGTCCTGGTAGAGTTAGGTACATCGTTAATTCTATCTTTGATATTTCTTAGGGAAATATCTGGACCACATCTAACTAGGCTAGGCCATTCTTCCAGCTCTCTTGTCTTGCAAACCCCTTCTTGAATGGAGATTATTTCAAGTCTTATGCATAAATAGAAGCGCAATACGCTTCAATCCCAAAATCTTGTATATTTATCCCCGAGCTCTGGATTGAAAAAAGAAACCATTACAACTTTTTAGAAATAGAAAACTGAAAGCAAAACATTATAGGACAAGCTCTGACTAATTTGGTTTAGTTTAATACGTCATACGTGTAGTTTAGTTACGCATTTCAGAGTACCATACTTTGCAGTAGCTTGGTTAAATTTCATTTTTACCTGAATAATTTTTCTTTTTTGTTTTCCAAGTTCATCAGAGCTCCACATGCACTTTAAATCTTCTCTTATTTCTCCCTTCTGCCTCTTCATTGCATAGGTACATTCACTCTCTCTAACCTTGGAATGTTTGGTGTGGATCGATTCGATGCAATTCTGCCACCCGGAACTGTTAGTATCGTTACTCTTCTACTTTTCTCTTCCCATTTTGTTCTCGGCCGGAGTACCTTTCTGGCATATACCTCTCTTCTCTTAGCCTAGTTATTAATATTAGGGACCTAATATATGTAGGGAGCAATCATGGCAGTAGGAGCATCTATCCCCACGGTTGTGGGTACTAAAGATGGTCGGATTGGCAAGAAAAACCAAATGCAGGTAACAAACTCGGATTATTCTTACTGTACATTTTAGAGAGTAGGCCTCTCTGTATCCTTATTTTTTGGATCTTGAATGTAGGTAAATGTAACAGCAGATCATCGAGTAATCTACGGTGCGGATCTTGCTACTTTCTTGCAAACCTTGGCAAAAATCATTGAGGACCCTAAAGACCTAACACTATAG

mRNA sequence

ATGGCTCATCTTCTCAACACCTCCTTCGTCGCCGCCTCCTCCCCATCTCTCCGGCGGAGGCCTTTTCTCCGACACCCATTTCACCCTACACGGCGGTCTCTTCAAGTCCAGGCCAAGATCCGCGAGATCTTCATGCCTGCTCTCAGCTCCACCATGACCGAGGGCAAGATTGTGTCCTGGATCAAGACTGAAGGCGACAAGCTCGCCAAGGGCGAGAGTGTTGTCGTCGTTGAGTCCGATAAGGCCGATATGGACGTCGAGACTTTCTATGATGGCTATCTTGCTGCCATTATGGTTGATGAAGGAGGCGTTGCGCCTGTTGGATCTGCAATCGCGCTTTTGGCTGAGACTGAGGATGAGATTTCTGAGGCTAAGTCTAAAGCTGCCATCTCTTCTGCTTCTCCGGCTTCGGCACCTCCGCCGGAGAAGAGTCCTGACAATGCAGCGGCAGTACCGGTTGCAGCGGCGGCACCAGTTTCGGTGGCGAAGGCGGCGAAGGTGGCGAAGGTGGTAGTGGCTTCCACTCACCCTGCATCTGAAGGGGGGAAGAGAATTGTGGCGTCTCCGTATGCGAAGAAGCTGGCTAAGGAGTTGAATGTGGAGTTGGCGACGGTGGTGGGGACTGGGCCATTGGGGAGAATTGTGGCTAAGGATGTTGAAGCTGCGGCTTCTGCTGCTGCTAGCTCAGTTTCGGCTCCGGGAGGCGGTGTGAAGCCCGCCCCATCCCTGGAGTTGGGAACCACTGTGCCGTTTACCACAATGCAAGGGGCGGTGAGTAGGAATATGGTTGAGAGTCTCGCGGTGCCCACTTTCAGAGTGGGGTATACCATTACAACAGATGCGCTTGATTCTCTTTACAAAAAGATTAAATCTAAGGGAGTGACCATGACAGCGTTGCTTGCCAAGGCTACAGCTCTAGCATTGGCTAAACATCCGGTAGTAAATTCAAGTTGTAGGGATGGTAAGAGCTTTACTTATAATAGAAGTATCAATATTGCAGTTGCAGTTGCCATTGATGGTGGCTTGATCACACCAGTGCTTCAAGATGCGGATAAGGTGGACATTTATTCTCTGTCTCGAAAGTGGAAGGAGTTAGTTGATAAGGCTAGAGCCAAGCAGCTGCAACCTCAGGAATACAACACTGGTACATTCACTCTCTCTAACCTTGGAATGTTTGGTGTGGATCGATTCGATGCAATTCTGCCACCCGGAACTGGAGCAATCATGGCAGTAGGAGCATCTATCCCCACGGTTGTGGGTACTAAAGATGGTCGGATTGGCAAGAAAAACCAAATGCAGGTAAATGTAACAGCAGATCATCGAGTAATCTACGGTGCGGATCTTGCTACTTTCTTGCAAACCTTGGCAAAAATCATTGAGGACCCTAAAGACCTAACACTATAG

Coding sequence (CDS)

ATGGCTCATCTTCTCAACACCTCCTTCGTCGCCGCCTCCTCCCCATCTCTCCGGCGGAGGCCTTTTCTCCGACACCCATTTCACCCTACACGGCGGTCTCTTCAAGTCCAGGCCAAGATCCGCGAGATCTTCATGCCTGCTCTCAGCTCCACCATGACCGAGGGCAAGATTGTGTCCTGGATCAAGACTGAAGGCGACAAGCTCGCCAAGGGCGAGAGTGTTGTCGTCGTTGAGTCCGATAAGGCCGATATGGACGTCGAGACTTTCTATGATGGCTATCTTGCTGCCATTATGGTTGATGAAGGAGGCGTTGCGCCTGTTGGATCTGCAATCGCGCTTTTGGCTGAGACTGAGGATGAGATTTCTGAGGCTAAGTCTAAAGCTGCCATCTCTTCTGCTTCTCCGGCTTCGGCACCTCCGCCGGAGAAGAGTCCTGACAATGCAGCGGCAGTACCGGTTGCAGCGGCGGCACCAGTTTCGGTGGCGAAGGCGGCGAAGGTGGCGAAGGTGGTAGTGGCTTCCACTCACCCTGCATCTGAAGGGGGGAAGAGAATTGTGGCGTCTCCGTATGCGAAGAAGCTGGCTAAGGAGTTGAATGTGGAGTTGGCGACGGTGGTGGGGACTGGGCCATTGGGGAGAATTGTGGCTAAGGATGTTGAAGCTGCGGCTTCTGCTGCTGCTAGCTCAGTTTCGGCTCCGGGAGGCGGTGTGAAGCCCGCCCCATCCCTGGAGTTGGGAACCACTGTGCCGTTTACCACAATGCAAGGGGCGGTGAGTAGGAATATGGTTGAGAGTCTCGCGGTGCCCACTTTCAGAGTGGGGTATACCATTACAACAGATGCGCTTGATTCTCTTTACAAAAAGATTAAATCTAAGGGAGTGACCATGACAGCGTTGCTTGCCAAGGCTACAGCTCTAGCATTGGCTAAACATCCGGTAGTAAATTCAAGTTGTAGGGATGGTAAGAGCTTTACTTATAATAGAAGTATCAATATTGCAGTTGCAGTTGCCATTGATGGTGGCTTGATCACACCAGTGCTTCAAGATGCGGATAAGGTGGACATTTATTCTCTGTCTCGAAAGTGGAAGGAGTTAGTTGATAAGGCTAGAGCCAAGCAGCTGCAACCTCAGGAATACAACACTGGTACATTCACTCTCTCTAACCTTGGAATGTTTGGTGTGGATCGATTCGATGCAATTCTGCCACCCGGAACTGGAGCAATCATGGCAGTAGGAGCATCTATCCCCACGGTTGTGGGTACTAAAGATGGTCGGATTGGCAAGAAAAACCAAATGCAGGTAAATGTAACAGCAGATCATCGAGTAATCTACGGTGCGGATCTTGCTACTTTCTTGCAAACCTTGGCAAAAATCATTGAGGACCCTAAAGACCTAACACTATAG

Protein sequence

MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSWIKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDEISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASEGGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASAAASSVSAPGGGVKPAPSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTALLAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLSRKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVVGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL
Homology
BLAST of Lsi02G019410 vs. ExPASy Swiss-Prot
Match: Q9C8P0 (Dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=EMB3003 PE=2 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 7.0e-182
Identity = 356/466 (76.39%), Postives = 388/466 (83.26%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           M+ LL T F+ + S   + R  +   F    R + +QAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MSRLLQTPFLPSVSLPTKTRSSVT-GFRVKPRIIPIQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           +K+EGDKL KGESVVVVESDKADMDVETFYDGYLAAIMV+EGGVAPVGSAIALLAETEDE
Sbjct: 61  VKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIMVEEGGVAPVGSAIALLAETEDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           I++AK+KA+       S  PP   P  A   PV+    V+ A  + +  V  ++ HPASE
Sbjct: 121 IADAKAKASGGGGGGDSKAPPASPPTAAVEAPVSVEKKVAAAPVS-IKAVAASAVHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASAAASSVSAPGGGVKPA 240
           GGKRIVASPYAKKLAKEL VELA +VG+GP+GRIVAKDVEA A+      +     V  A
Sbjct: 181 GGKRIVASPYAKKLAKELKVELAGLVGSGPMGRIVAKDVEAVAAGGGVQAAVAVKEVVAA 240

Query: 241 PSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTALL 300
           P +ELG+ VPFTTMQGAVSRNMVESL VPTFRVGYTI+TDALD+LYKKIKSKGVTMTALL
Sbjct: 241 PGVELGSVVPFTTMQGAVSRNMVESLGVPTFRVGYTISTDALDALYKKIKSKGVTMTALL 300

Query: 301 AKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLSR 360
           AKATALALAKHPVVNSSCRDG SF YN SIN+AVAVAIDGGLITPVLQ+ADKVDIYSLSR
Sbjct: 301 AKATALALAKHPVVNSSCRDGNSFVYNSSINVAVAVAIDGGLITPVLQNADKVDIYSLSR 360

Query: 361 KWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVVG 420
           KWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGAS P+VV 
Sbjct: 361 KWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASQPSVVA 420

Query: 421 TKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLT 467
           TKDGRIG KNQMQVNVTADHRVIYGADLA FLQTLA IIEDPKDLT
Sbjct: 421 TKDGRIGMKNQMQVNVTADHRVIYGADLAQFLQTLASIIEDPKDLT 464

BLAST of Lsi02G019410 vs. ExPASy Swiss-Prot
Match: Q9SQI8 (Dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogenase complex, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LTA2 PE=2 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.2e-152
Identity = 315/477 (66.04%), Postives = 357/477 (74.84%), Query Frame = 0

Query: 6   NTSFVAASSPSLRRRPF-LRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSWIKTE 65
           N SF ++ SPSLR   F    P    RRS+ V++KIREIFMPALSSTMTEGKIVSWIKTE
Sbjct: 20  NISFASSVSPSLRSVVFRSTTPATSHRRSMTVRSKIREIFMPALSSTMTEGKIVSWIKTE 79

Query: 66  GDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDEISEA 125
           G+KLAKGESVVVVESDKADMDVETFYDGYLAAI+V EG  APVG+AI LLAETE EI EA
Sbjct: 80  GEKLAKGESVVVVESDKADMDVETFYDGYLAAIVVGEGETAPVGAAIGLLAETEAEIEEA 139

Query: 126 KSKAAI-SSASPASAPPPEKSPDNAAAVP-VAAAAPVSVAKAAKVAKVVVASTHPASEGG 185
           KSKAA  SS+S A A  P   P  ++  P +A  APV+                  S+G 
Sbjct: 140 KSKAASKSSSSVAEAVVPSPPPVTSSPAPAIAQPAPVTA----------------VSDGP 199

Query: 186 KRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASAAASSVSAPGGGVKPAPS 245
           ++ VA+PYAKKLAK+  V++ +V GTGP GRI A DVE AA  A S  S       P P 
Sbjct: 200 RKTVATPYAKKLAKQHKVDIESVAGTGPFGRITASDVETAAGIAPSKSSIAPPPPPPPPV 259

Query: 246 LELGTT------------VPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIK 305
               TT            VPFT MQ AVS+NM+ESL+VPTFRVGY + TDALD+LY+K+K
Sbjct: 260 TAKATTTNLPPLLPDSSIVPFTAMQSAVSKNMIESLSVPTFRVGYPVNTDALDALYEKVK 319

Query: 306 SKGVTMTALLAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDA 365
            KGVTMTALLAKA  +ALA+HPVVN+SC+DGKSF+YN SINIAVAVAI+GGLITPVLQDA
Sbjct: 320 PKGVTMTALLAKAAGMALAQHPVVNASCKDGKSFSYNSSINIAVAVAINGGLITPVLQDA 379

Query: 366 DKVDIYSLSRKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMA 425
           DK+D+Y LS+KWKELV KAR+KQLQP EYN+GTFTLSNLGMFGVDRFDAILPPG GAIMA
Sbjct: 380 DKLDLYLLSQKWKELVGKARSKQLQPHEYNSGTFTLSNLGMFGVDRFDAILPPGQGAIMA 439

Query: 426 VGASIPTVVGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           VGAS PTVV  KDG    KN M VNVTADHR++YGADLA FLQT AKIIE+P  LTL
Sbjct: 440 VGASKPTVVADKDGFFSVKNTMLVNVTADHRIVYGADLAAFLQTFAKIIENPDSLTL 480

BLAST of Lsi02G019410 vs. ExPASy Swiss-Prot
Match: Q8RWN9 (Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g13930 PE=1 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.7e-50
Identity = 161/440 (36.59%), Postives = 241/440 (54.77%), Query Frame = 0

Query: 41  REIFMPALSSTMTEGKIVSWIKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVD 100
           +EI MP+LS TMTEG I  W+K EGDK+A GE +  VE+DKA +++E   +G+LA I+ +
Sbjct: 112 QEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKE 171

Query: 101 EGG-VAPVGSAIALLAETEDEISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPV 160
           EG     VG  IA+  E ED+I + K     S   PA+   PE  P    A  +     V
Sbjct: 172 EGAKEIQVGEVIAITVEDEDDIQKFKDYTPSSDTGPAA---PEAKP----APSLPKEEKV 231

Query: 161 SVAKAAKVAKVVVASTHPASEGGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDV 220
               +A  AK+   S+ P+ +   RI ASP A+KLA++ NV L+++ GTGP GRIV  DV
Sbjct: 232 EKPASAPEAKISKPSSAPSED---RIFASPLARKLAEDNNVPLSSIKGTGPEGRIVKADV 291

Query: 221 E-AAASAAASSVSAPGGGV-KPAPSLELGTTVPFTTMQG-AVSRNMVESLAVPTFRVGYT 280
           E   AS +  + + P   V    P+L+    +P T ++    SR       +P + +   
Sbjct: 292 EDFLASGSKETTAKPSKQVDSKVPALDY-VDIPHTQIRKVTASRLAFSKQTIPHYYLTVD 351

Query: 281 ITTDALDSLYKKIKS-------KGVTMTALLAKATALALAKHPVVNSSCRDGKSFTYNRS 340
              D +  L  ++ S       K +++  L+ KA ALAL K P  NSS  D +     ++
Sbjct: 352 TCVDKMMGLRSQLNSFQEASGGKRISVNDLVIKAAALALRKVPQCNSSWTD-EYIRQFKN 411

Query: 341 INIAVAVAIDGGLITPVLQDADKVDIYSLSRKWKELVDKARAKQLQPQEYNTGTFTLSNL 400
           +NI VAV  + GL  PV++DADK  + ++  + + L  KA+   L+P++Y  GTFT+SNL
Sbjct: 412 VNINVAVQTENGLYVPVVKDADKKGLSTIGEEVRFLAQKAKENSLKPEDYEGGTFTVSNL 471

Query: 401 -GMFGVDRFDAILPPGTGAIMAVGASIPTVV-GTKDGRIGKKNQMQVNVTADHRVIYGAD 460
            G FG+ +F A++ P   AI+A+G++   VV GT   +    + M V ++ DHRVI GA 
Sbjct: 472 GGPFGIKQFCAVINPPQAAILAIGSAEKRVVPGTGPDQYNVASYMSVTLSCDHRVIDGAI 531

Query: 461 LATFLQTLAKIIEDPKDLTL 468
            A +L+     IE P+ + L
Sbjct: 532 GAEWLKAFKGYIETPESMLL 539

BLAST of Lsi02G019410 vs. ExPASy Swiss-Prot
Match: Q9R9N3 (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Rhizobium meliloti (strain 1021) OX=266834 GN=pdhC PE=3 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.8e-50
Identity = 160/450 (35.56%), Postives = 244/450 (54.22%), Query Frame = 0

Query: 43  IFMPALSSTMTEGKIVSWIKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEG 102
           I MPALS TM EG +  W+  EGDK+  G+ +  +E+DKA M+VE   +G +A I+V  G
Sbjct: 5   ITMPALSPTMEEGNLAKWLVKEGDKVKSGDVIAEIETDKATMEVEAVDEGTVAKIVVPAG 64

Query: 103 --GVAPVGSAIALLAETEDEISEAKSKAAISSASPASAPPPEKSPDNA-AAVPVAAAAPV 162
             GV  V + IA+LA   ++++ A +K    +A    AP P+++ + A AA P  AAAP 
Sbjct: 65  TEGV-KVNALIAVLAAEGEDVATA-AKGGNGAAGAVPAPKPKETAETAPAAAPAPAAAPA 124

Query: 163 SVAKAAKVAKVVVASTHPASEGGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDV 222
             A A        AS  PA   GKRI +SP A++LAKE  ++L+ + G+GP GR+V KDV
Sbjct: 125 PQAAAP-------ASPAPADGEGKRIFSSPLARRLAKEAGIDLSAIAGSGPHGRVVKKDV 184

Query: 223 EAAAS-AAASSVSAPGGGVKPAPSLELGTT---------------VPFTTMQGAVSRNMV 282
           E A S  AA    AP     PA +L  G +               VP   M+  +++ +V
Sbjct: 185 ETAVSGGAAKPAGAPAAAPAPA-TLAKGMSEDAVLKLFEPGSYELVPHDGMRKTIAKRLV 244

Query: 283 ES-LAVPTFRVGYTITTDALDSLYKKIKSKG----------VTMTALLAKATALALAKHP 342
           ES   +P F V      DAL +L  ++ +            +++  ++ KA ALAL   P
Sbjct: 245 ESKQTIPHFYVSVDCELDALMALRAQLNAAAPEKDGKPVYKLSVNDMVIKALALALRDVP 304

Query: 343 VVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLSRKWKELVDKARAK 402
             N S  D ++   ++  ++ VAV+I GGLITP+++ A+   + ++S + K+L  +A+ +
Sbjct: 305 DANVSWTD-QNMVKHKHADVGVAVSIPGGLITPIVRQAELKSLSAISNEMKDLGKRAKER 364

Query: 403 QLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVVGTKDGRIGKKNQM 462
           +L+P+EY  GT  +SN+GM GV  F A++ P    I+AVGA    VV  ++  +   N M
Sbjct: 365 KLKPEEYQGGTTAVSNMGMMGVKDFAAVVNPPHATILAVGAGEDRVV-VRNKEMVIANVM 424

BLAST of Lsi02G019410 vs. ExPASy Swiss-Prot
Match: Q5M729 (Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g54220 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 8.3e-50
Identity = 160/446 (35.87%), Postives = 238/446 (53.36%), Query Frame = 0

Query: 41  REIFMPALSSTMTEGKIVSWIKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVD 100
           +EI MP+LS TMTEG I  W+K EGDK+A GE +  VE+DKA +++E   +GYLA I+  
Sbjct: 112 QEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKA 171

Query: 101 EGG-VAPVGSAIALLAETEDEISEAK-------SKAAISSASPASAPPPEKSPDNAAAVP 160
           EG     VG  IA+  E E++I + K       + AA + A P  APP E+     ++ P
Sbjct: 172 EGSKEIQVGEVIAITVEDEEDIGKFKDYTPSSTADAAPTKAEPTPAPPKEEKVKQPSSPP 231

Query: 161 VAAAAPVSVAKAAKVAKVVVASTHPASEGGKRIVASPYAKKLAKELNVELATVVGTGPLG 220
                     KA+K       ST P    G R+ ASP A+KLA++ NV L+ + GTGP G
Sbjct: 232 --------EPKASK------PSTPPT---GDRVFASPLARKLAEDNNVPLSDIEGTGPEG 291

Query: 221 RIVAKDV-EAAASAAASSVSAPGGGV-KPAPSLELGTTVPFTTMQGAVSRNMVESLAVPT 280
           RIV  D+ E  AS+   + + P       AP+L+          +   SR       +P 
Sbjct: 292 RIVKADIDEYLASSGKGATAKPSKSTDSKAPALDYVDIPHSQIRKVTASRLAFSKQTIPH 351

Query: 281 FRVGYTITTDALDSLYKKIKS-------KGVTMTALLAKATALALAKHPVVNSSCRDGKS 340
           + +      D L +L  ++ S       K +++  L+ KA ALAL K P  NSS  D   
Sbjct: 352 YYLTVDTCVDKLMALRSQLNSFKEASGGKRISVNDLVVKAAALALRKVPQCNSSWTDDYI 411

Query: 341 FTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLSRKWKELVDKARAKQLQPQEYNTGT 400
             + +++NI VAV  + GL  PV++DAD+  + ++  + + L  KA+   L+P++Y  GT
Sbjct: 412 RQF-KNVNINVAVQTENGLYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPEDYEGGT 471

Query: 401 FTLSNL-GMFGVDRFDAILPPGTGAIMAVGASIPTVV-GTKDGRIGKKNQMQVNVTADHR 460
           FT+SNL G FG+ +F A++ P   AI+AVG++   VV G    +    + M V ++ DHR
Sbjct: 472 FTVSNLGGPFGIKQFCAVVNPPQAAILAVGSAEKRVVPGNGPDQFNFASYMPVTLSCDHR 531

Query: 461 VIYGADLATFLQTLAKIIEDPKDLTL 468
           V+ GA  A +L+     IE+PK + L
Sbjct: 532 VVDGAIGAEWLKAFKGYIENPKSMLL 539

BLAST of Lsi02G019410 vs. ExPASy TrEMBL
Match: A0A0A0K7V1 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis sativus OX=3659 GN=Csa_7G284430 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 4.0e-225
Identity = 437/468 (93.38%), Postives = 445/468 (95.09%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSF+ ASSPSLR  PFL   FH  RR LQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFLPASSPSLRPTPFLPPSFHSIRRPLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAET+DE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETQDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           ISEAKS+AA  SASPASAPPP+KSP+N  A P   AAPV VAKAA  A VVVASTHPASE
Sbjct: 121 ISEAKSRAANPSASPASAPPPDKSPENVVATP---AAPVMVAKAA-AAPVVVASTHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAA-SAAASSVSAPGGGVKP 240
           GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAA SAAAS+VSAPGGGVKP
Sbjct: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAATSAAASAVSAPGGGVKP 240

Query: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTAL 300
           APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKSKGVTMTAL
Sbjct: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKSKGVTMTAL 300

Query: 301 LAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360
           LAKATALALAKHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDADKVDIYSLS
Sbjct: 301 LAKATALALAKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360

Query: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420
           RKWKELV+KARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV
Sbjct: 361 RKWKELVEKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420

Query: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL
Sbjct: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 464

BLAST of Lsi02G019410 vs. ExPASy TrEMBL
Match: A0A5D3CET8 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold39G00290 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.8e-224
Identity = 435/468 (92.95%), Postives = 442/468 (94.44%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSF+ ASSPSLR  PFL  PFHP RR LQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFLPASSPSLRPTPFLPPPFHPMRRPLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAET+DE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETQDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           ISEAKS+AA  SASPASAPPP+KSP+N  A P   AA V VAKAA  A VVV+STHPASE
Sbjct: 121 ISEAKSRAANPSASPASAPPPDKSPENVVATP---AAQVVVAKAA-AAPVVVSSTHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE-AAASAAASSVSAPGGGVKP 240
           GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE AAASAAASSVSAPGGGVKP
Sbjct: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAAASAAASSVSAPGGGVKP 240

Query: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTAL 300
             SLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKSKGVTMTAL
Sbjct: 241 TTSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKSKGVTMTAL 300

Query: 301 LAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360
           LAKATALALAKHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDADKVDIYSLS
Sbjct: 301 LAKATALALAKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360

Query: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420
           RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV
Sbjct: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420

Query: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           GTKDGRIGKKNQMQVNVTADHRVIYGADLATFL T AKIIEDPKDLTL
Sbjct: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLHTFAKIIEDPKDLTL 464

BLAST of Lsi02G019410 vs. ExPASy TrEMBL
Match: A0A1S3CG29 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo OX=3656 GN=LOC103500056 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 8.4e-223
Identity = 433/468 (92.52%), Postives = 441/468 (94.23%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSF+ ASSPSLR  PFL  PFHP RR LQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFLPASSPSLRPTPFLPPPFHPMRRPLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAET+DE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETQDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           ISEAKS+AA  SASPASAPPP+KSP+N  A P   AA V VAKAA  A VVV+STHPASE
Sbjct: 121 ISEAKSRAANPSASPASAPPPDKSPENVVATP---AAQVVVAKAA-AAPVVVSSTHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE-AAASAAASSVSAPGGGVKP 240
           GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE AAASAAASSVSAPGGGVKP
Sbjct: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAAASAAASSVSAPGGGVKP 240

Query: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTAL 300
             SLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKSKGVTMTAL
Sbjct: 241 TTSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKSKGVTMTAL 300

Query: 301 LAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360
           LAKATALALAKHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDADKVDIYSLS
Sbjct: 301 LAKATALALAKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360

Query: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420
           RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV
Sbjct: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420

Query: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           GTKDGRIG +NQMQVNVTADHRVIYGADLATFL T AKIIEDPKDLTL
Sbjct: 421 GTKDGRIGXENQMQVNVTADHRVIYGADLATFLHTFAKIIEDPKDLTL 464

BLAST of Lsi02G019410 vs. ExPASy TrEMBL
Match: A0A5A7V6B9 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold455G001250 PE=3 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.4e-222
Identity = 435/469 (92.75%), Postives = 442/469 (94.24%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSF+ ASSPSLR  PFL  PFHP RR LQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFLPASSPSLRPTPFLPPPFHPMRRPLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAET+DE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETQDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           ISEAKS+AA  SASPASAPPP+KSP+N  A P   AA V VAKAA  A VVV+STHPASE
Sbjct: 121 ISEAKSRAANPSASPASAPPPDKSPENVVATP---AAQVVVAKAA-AAPVVVSSTHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE-AAASAAASSVSAPGGGVKP 240
           GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE AAASAAASSVSAPGGGVKP
Sbjct: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAAASAAASSVSAPGGGVKP 240

Query: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTAL 300
             SLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKSKGVTMTAL
Sbjct: 241 TTSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKSKGVTMTAL 300

Query: 301 LAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360
           LAKATALALAKHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDADKVDIYSLS
Sbjct: 301 LAKATALALAKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360

Query: 361 RKWKELVDKARAKQLQPQEYNT-GTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTV 420
           RKWKELVDKARAKQLQPQEYNT GTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTV
Sbjct: 361 RKWKELVDKARAKQLQPQEYNTAGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTV 420

Query: 421 VGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           VGTKDGRIGKKNQMQVNVTADHRVIYGADLATFL T AKIIEDPKDLTL
Sbjct: 421 VGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLHTFAKIIEDPKDLTL 465

BLAST of Lsi02G019410 vs. ExPASy TrEMBL
Match: A0A6J1F217 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita moschata OX=3662 GN=LOC111439040 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 3.0e-220
Identity = 433/476 (90.97%), Postives = 441/476 (92.65%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSFVA SSPSLRR PFLR PFHP RRSLQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFVAVSSPSLRRGPFLRPPFHPRRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120

Query: 121 ISEAKSKAA---ISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHP 180
           IS AKS+AA    +SAS +++P  EK P+N  A   A AAPV+VAKA   A VVVASTHP
Sbjct: 121 ISVAKSRAADPSSASASASASPASEKIPENVGA---ATAAPVAVAKA---APVVVASTHP 180

Query: 181 ASEGGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASA------AASSVS 240
           ASEGGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASA      AA  VS
Sbjct: 181 ASEGGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASAAVSVSDAAKPVS 240

Query: 241 APGGGVKPAPSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKS 300
           APG   KPAPSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKS
Sbjct: 241 APGSSTKPAPSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKS 300

Query: 301 KGVTMTALLAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDAD 360
           KGVTMTALLAKATALALAKHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDAD
Sbjct: 301 KGVTMTALLAKATALALAKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDAD 360

Query: 361 KVDIYSLSRKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAV 420
           KVDIYSLSRKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAV
Sbjct: 361 KVDIYSLSRKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAV 420

Query: 421 GASIPTVVGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           GASIPTVVGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL
Sbjct: 421 GASIPTVVGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 470

BLAST of Lsi02G019410 vs. NCBI nr
Match: XP_038899200.1 (dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex, chloroplastic [Benincasa hispida])

HSP 1 Score: 810.4 bits (2092), Expect = 7.8e-231
Identity = 443/467 (94.86%), Postives = 447/467 (95.72%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSFVAASSPSLRRRPFLR PFHP+RRSLQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFVAASSPSLRRRPFLRPPFHPSRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           ISEAKSKAAIS ASPASAPPPEKSP       +A AAP +   AAK A VVVASTHPASE
Sbjct: 121 ISEAKSKAAISPASPASAPPPEKSP------AIAVAAPAAPVAAAKAAPVVVASTHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASAAASSVSAPGGGVKPA 240
            GKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASAAASSV APGGGVKPA
Sbjct: 181 EGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASAAASSVPAPGGGVKPA 240

Query: 241 PSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTALL 300
           PSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKSKGVTMTALL
Sbjct: 241 PSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKSKGVTMTALL 300

Query: 301 AKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLSR 360
           AKATALAL KHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDADKVDIYSLSR
Sbjct: 301 AKATALALTKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDADKVDIYSLSR 360

Query: 361 KWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVVG 420
           KWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVVG
Sbjct: 361 KWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVVG 420

Query: 421 TKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           TKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL
Sbjct: 421 TKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 461

BLAST of Lsi02G019410 vs. NCBI nr
Match: XP_004150399.1 (dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex, chloroplastic [Cucumis sativus] >KGN44417.1 hypothetical protein Csa_016594 [Cucumis sativus])

HSP 1 Score: 790.4 bits (2040), Expect = 8.4e-225
Identity = 437/468 (93.38%), Postives = 445/468 (95.09%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSF+ ASSPSLR  PFL   FH  RR LQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFLPASSPSLRPTPFLPPSFHSIRRPLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAET+DE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETQDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           ISEAKS+AA  SASPASAPPP+KSP+N  A P   AAPV VAKAA  A VVVASTHPASE
Sbjct: 121 ISEAKSRAANPSASPASAPPPDKSPENVVATP---AAPVMVAKAA-AAPVVVASTHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAA-SAAASSVSAPGGGVKP 240
           GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAA SAAAS+VSAPGGGVKP
Sbjct: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAATSAAASAVSAPGGGVKP 240

Query: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTAL 300
           APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKSKGVTMTAL
Sbjct: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKSKGVTMTAL 300

Query: 301 LAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360
           LAKATALALAKHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDADKVDIYSLS
Sbjct: 301 LAKATALALAKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360

Query: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420
           RKWKELV+KARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV
Sbjct: 361 RKWKELVEKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420

Query: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL
Sbjct: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 464

BLAST of Lsi02G019410 vs. NCBI nr
Match: TYK09860.1 (dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex [Cucumis melo var. makuwa])

HSP 1 Score: 786.6 bits (2030), Expect = 1.2e-223
Identity = 435/468 (92.95%), Postives = 442/468 (94.44%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSF+ ASSPSLR  PFL  PFHP RR LQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFLPASSPSLRPTPFLPPPFHPMRRPLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAET+DE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETQDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           ISEAKS+AA  SASPASAPPP+KSP+N  A P   AA V VAKAA  A VVV+STHPASE
Sbjct: 121 ISEAKSRAANPSASPASAPPPDKSPENVVATP---AAQVVVAKAA-AAPVVVSSTHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE-AAASAAASSVSAPGGGVKP 240
           GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE AAASAAASSVSAPGGGVKP
Sbjct: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAAASAAASSVSAPGGGVKP 240

Query: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTAL 300
             SLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKSKGVTMTAL
Sbjct: 241 TTSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKSKGVTMTAL 300

Query: 301 LAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360
           LAKATALALAKHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDADKVDIYSLS
Sbjct: 301 LAKATALALAKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360

Query: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420
           RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV
Sbjct: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420

Query: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           GTKDGRIGKKNQMQVNVTADHRVIYGADLATFL T AKIIEDPKDLTL
Sbjct: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLHTFAKIIEDPKDLTL 464

BLAST of Lsi02G019410 vs. NCBI nr
Match: XP_008461468.1 (PREDICTED: LOW QUALITY PROTEIN: dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex, chloroplastic [Cucumis melo])

HSP 1 Score: 782.7 bits (2020), Expect = 1.7e-222
Identity = 433/468 (92.52%), Postives = 441/468 (94.23%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSF+ ASSPSLR  PFL  PFHP RR LQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFLPASSPSLRPTPFLPPPFHPMRRPLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAET+DE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETQDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           ISEAKS+AA  SASPASAPPP+KSP+N  A P   AA V VAKAA  A VVV+STHPASE
Sbjct: 121 ISEAKSRAANPSASPASAPPPDKSPENVVATP---AAQVVVAKAA-AAPVVVSSTHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE-AAASAAASSVSAPGGGVKP 240
           GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE AAASAAASSVSAPGGGVKP
Sbjct: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAAASAAASSVSAPGGGVKP 240

Query: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTAL 300
             SLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKSKGVTMTAL
Sbjct: 241 TTSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKSKGVTMTAL 300

Query: 301 LAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360
           LAKATALALAKHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDADKVDIYSLS
Sbjct: 301 LAKATALALAKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360

Query: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420
           RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV
Sbjct: 361 RKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVV 420

Query: 421 GTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           GTKDGRIG +NQMQVNVTADHRVIYGADLATFL T AKIIEDPKDLTL
Sbjct: 421 GTKDGRIGXENQMQVNVTADHRVIYGADLATFLHTFAKIIEDPKDLTL 464

BLAST of Lsi02G019410 vs. NCBI nr
Match: KAA0061281.1 (dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogenase complex [Cucumis melo var. makuwa])

HSP 1 Score: 781.9 bits (2018), Expect = 3.0e-222
Identity = 435/469 (92.75%), Postives = 442/469 (94.24%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           MAHLLNTSF+ ASSPSLR  PFL  PFHP RR LQVQAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MAHLLNTSFLPASSPSLRPTPFLPPPFHPMRRPLQVQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAET+DE
Sbjct: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETQDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           ISEAKS+AA  SASPASAPPP+KSP+N  A P   AA V VAKAA  A VVV+STHPASE
Sbjct: 121 ISEAKSRAANPSASPASAPPPDKSPENVVATP---AAQVVVAKAA-AAPVVVSSTHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE-AAASAAASSVSAPGGGVKP 240
           GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVE AAASAAASSVSAPGGGVKP
Sbjct: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAAASAAASSVSAPGGGVKP 240

Query: 241 APSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTAL 300
             SLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALD+LYKKIKSKGVTMTAL
Sbjct: 241 TTSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDALYKKIKSKGVTMTAL 300

Query: 301 LAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360
           LAKATALALAKHPVVNSSCRDGKSFTYN SINIAVAVAIDGGLITPVLQDADKVDIYSLS
Sbjct: 301 LAKATALALAKHPVVNSSCRDGKSFTYNSSINIAVAVAIDGGLITPVLQDADKVDIYSLS 360

Query: 361 RKWKELVDKARAKQLQPQEYNT-GTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTV 420
           RKWKELVDKARAKQLQPQEYNT GTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTV
Sbjct: 361 RKWKELVDKARAKQLQPQEYNTAGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTV 420

Query: 421 VGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           VGTKDGRIGKKNQMQVNVTADHRVIYGADLATFL T AKIIEDPKDLTL
Sbjct: 421 VGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLHTFAKIIEDPKDLTL 465

BLAST of Lsi02G019410 vs. TAIR 10
Match: AT1G34430.1 (2-oxoacid dehydrogenases acyltransferase family protein )

HSP 1 Score: 638.3 bits (1645), Expect = 4.9e-183
Identity = 356/466 (76.39%), Postives = 388/466 (83.26%), Query Frame = 0

Query: 1   MAHLLNTSFVAASSPSLRRRPFLRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSW 60
           M+ LL T F+ + S   + R  +   F    R + +QAKIREIFMPALSSTMTEGKIVSW
Sbjct: 1   MSRLLQTPFLPSVSLPTKTRSSVT-GFRVKPRIIPIQAKIREIFMPALSSTMTEGKIVSW 60

Query: 61  IKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDE 120
           +K+EGDKL KGESVVVVESDKADMDVETFYDGYLAAIMV+EGGVAPVGSAIALLAETEDE
Sbjct: 61  VKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIMVEEGGVAPVGSAIALLAETEDE 120

Query: 121 ISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPVSVAKAAKVAKVVVASTHPASE 180
           I++AK+KA+       S  PP   P  A   PV+    V+ A  + +  V  ++ HPASE
Sbjct: 121 IADAKAKASGGGGGGDSKAPPASPPTAAVEAPVSVEKKVAAAPVS-IKAVAASAVHPASE 180

Query: 181 GGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASAAASSVSAPGGGVKPA 240
           GGKRIVASPYAKKLAKEL VELA +VG+GP+GRIVAKDVEA A+      +     V  A
Sbjct: 181 GGKRIVASPYAKKLAKELKVELAGLVGSGPMGRIVAKDVEAVAAGGGVQAAVAVKEVVAA 240

Query: 241 PSLELGTTVPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIKSKGVTMTALL 300
           P +ELG+ VPFTTMQGAVSRNMVESL VPTFRVGYTI+TDALD+LYKKIKSKGVTMTALL
Sbjct: 241 PGVELGSVVPFTTMQGAVSRNMVESLGVPTFRVGYTISTDALDALYKKIKSKGVTMTALL 300

Query: 301 AKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLSR 360
           AKATALALAKHPVVNSSCRDG SF YN SIN+AVAVAIDGGLITPVLQ+ADKVDIYSLSR
Sbjct: 301 AKATALALAKHPVVNSSCRDGNSFVYNSSINVAVAVAIDGGLITPVLQNADKVDIYSLSR 360

Query: 361 KWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASIPTVVG 420
           KWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGAS P+VV 
Sbjct: 361 KWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMAVGASQPSVVA 420

Query: 421 TKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLT 467
           TKDGRIG KNQMQVNVTADHRVIYGADLA FLQTLA IIEDPKDLT
Sbjct: 421 TKDGRIGMKNQMQVNVTADHRVIYGADLAQFLQTLASIIEDPKDLT 464

BLAST of Lsi02G019410 vs. TAIR 10
Match: AT3G25860.1 (2-oxoacid dehydrogenases acyltransferase family protein )

HSP 1 Score: 541.2 bits (1393), Expect = 8.2e-154
Identity = 315/477 (66.04%), Postives = 357/477 (74.84%), Query Frame = 0

Query: 6   NTSFVAASSPSLRRRPF-LRHPFHPTRRSLQVQAKIREIFMPALSSTMTEGKIVSWIKTE 65
           N SF ++ SPSLR   F    P    RRS+ V++KIREIFMPALSSTMTEGKIVSWIKTE
Sbjct: 20  NISFASSVSPSLRSVVFRSTTPATSHRRSMTVRSKIREIFMPALSSTMTEGKIVSWIKTE 79

Query: 66  GDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVDEGGVAPVGSAIALLAETEDEISEA 125
           G+KLAKGESVVVVESDKADMDVETFYDGYLAAI+V EG  APVG+AI LLAETE EI EA
Sbjct: 80  GEKLAKGESVVVVESDKADMDVETFYDGYLAAIVVGEGETAPVGAAIGLLAETEAEIEEA 139

Query: 126 KSKAAI-SSASPASAPPPEKSPDNAAAVP-VAAAAPVSVAKAAKVAKVVVASTHPASEGG 185
           KSKAA  SS+S A A  P   P  ++  P +A  APV+                  S+G 
Sbjct: 140 KSKAASKSSSSVAEAVVPSPPPVTSSPAPAIAQPAPVTA----------------VSDGP 199

Query: 186 KRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDVEAAASAAASSVSAPGGGVKPAPS 245
           ++ VA+PYAKKLAK+  V++ +V GTGP GRI A DVE AA  A S  S       P P 
Sbjct: 200 RKTVATPYAKKLAKQHKVDIESVAGTGPFGRITASDVETAAGIAPSKSSIAPPPPPPPPV 259

Query: 246 LELGTT------------VPFTTMQGAVSRNMVESLAVPTFRVGYTITTDALDSLYKKIK 305
               TT            VPFT MQ AVS+NM+ESL+VPTFRVGY + TDALD+LY+K+K
Sbjct: 260 TAKATTTNLPPLLPDSSIVPFTAMQSAVSKNMIESLSVPTFRVGYPVNTDALDALYEKVK 319

Query: 306 SKGVTMTALLAKATALALAKHPVVNSSCRDGKSFTYNRSINIAVAVAIDGGLITPVLQDA 365
            KGVTMTALLAKA  +ALA+HPVVN+SC+DGKSF+YN SINIAVAVAI+GGLITPVLQDA
Sbjct: 320 PKGVTMTALLAKAAGMALAQHPVVNASCKDGKSFSYNSSINIAVAVAINGGLITPVLQDA 379

Query: 366 DKVDIYSLSRKWKELVDKARAKQLQPQEYNTGTFTLSNLGMFGVDRFDAILPPGTGAIMA 425
           DK+D+Y LS+KWKELV KAR+KQLQP EYN+GTFTLSNLGMFGVDRFDAILPPG GAIMA
Sbjct: 380 DKLDLYLLSQKWKELVGKARSKQLQPHEYNSGTFTLSNLGMFGVDRFDAILPPGQGAIMA 439

Query: 426 VGASIPTVVGTKDGRIGKKNQMQVNVTADHRVIYGADLATFLQTLAKIIEDPKDLTL 468
           VGAS PTVV  KDG    KN M VNVTADHR++YGADLA FLQT AKIIE+P  LTL
Sbjct: 440 VGASKPTVVADKDGFFSVKNTMLVNVTADHRIVYGADLAAFLQTFAKIIENPDSLTL 480

BLAST of Lsi02G019410 vs. TAIR 10
Match: AT3G13930.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-51
Identity = 161/440 (36.59%), Postives = 241/440 (54.77%), Query Frame = 0

Query: 41  REIFMPALSSTMTEGKIVSWIKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVD 100
           +EI MP+LS TMTEG I  W+K EGDK+A GE +  VE+DKA +++E   +G+LA I+ +
Sbjct: 112 QEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKE 171

Query: 101 EGG-VAPVGSAIALLAETEDEISEAKSKAAISSASPASAPPPEKSPDNAAAVPVAAAAPV 160
           EG     VG  IA+  E ED+I + K     S   PA+   PE  P    A  +     V
Sbjct: 172 EGAKEIQVGEVIAITVEDEDDIQKFKDYTPSSDTGPAA---PEAKP----APSLPKEEKV 231

Query: 161 SVAKAAKVAKVVVASTHPASEGGKRIVASPYAKKLAKELNVELATVVGTGPLGRIVAKDV 220
               +A  AK+   S+ P+ +   RI ASP A+KLA++ NV L+++ GTGP GRIV  DV
Sbjct: 232 EKPASAPEAKISKPSSAPSED---RIFASPLARKLAEDNNVPLSSIKGTGPEGRIVKADV 291

Query: 221 E-AAASAAASSVSAPGGGV-KPAPSLELGTTVPFTTMQG-AVSRNMVESLAVPTFRVGYT 280
           E   AS +  + + P   V    P+L+    +P T ++    SR       +P + +   
Sbjct: 292 EDFLASGSKETTAKPSKQVDSKVPALDY-VDIPHTQIRKVTASRLAFSKQTIPHYYLTVD 351

Query: 281 ITTDALDSLYKKIKS-------KGVTMTALLAKATALALAKHPVVNSSCRDGKSFTYNRS 340
              D +  L  ++ S       K +++  L+ KA ALAL K P  NSS  D +     ++
Sbjct: 352 TCVDKMMGLRSQLNSFQEASGGKRISVNDLVIKAAALALRKVPQCNSSWTD-EYIRQFKN 411

Query: 341 INIAVAVAIDGGLITPVLQDADKVDIYSLSRKWKELVDKARAKQLQPQEYNTGTFTLSNL 400
           +NI VAV  + GL  PV++DADK  + ++  + + L  KA+   L+P++Y  GTFT+SNL
Sbjct: 412 VNINVAVQTENGLYVPVVKDADKKGLSTIGEEVRFLAQKAKENSLKPEDYEGGTFTVSNL 471

Query: 401 -GMFGVDRFDAILPPGTGAIMAVGASIPTVV-GTKDGRIGKKNQMQVNVTADHRVIYGAD 460
            G FG+ +F A++ P   AI+A+G++   VV GT   +    + M V ++ DHRVI GA 
Sbjct: 472 GGPFGIKQFCAVINPPQAAILAIGSAEKRVVPGTGPDQYNVASYMSVTLSCDHRVIDGAI 531

Query: 461 LATFLQTLAKIIEDPKDLTL 468
            A +L+     IE P+ + L
Sbjct: 532 GAEWLKAFKGYIETPESMLL 539

BLAST of Lsi02G019410 vs. TAIR 10
Match: AT1G54220.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 199.5 bits (506), Expect = 5.9e-51
Identity = 160/446 (35.87%), Postives = 238/446 (53.36%), Query Frame = 0

Query: 41  REIFMPALSSTMTEGKIVSWIKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVD 100
           +EI MP+LS TMTEG I  W+K EGDK+A GE +  VE+DKA +++E   +GYLA I+  
Sbjct: 112 QEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKA 171

Query: 101 EGG-VAPVGSAIALLAETEDEISEAK-------SKAAISSASPASAPPPEKSPDNAAAVP 160
           EG     VG  IA+  E E++I + K       + AA + A P  APP E+     ++ P
Sbjct: 172 EGSKEIQVGEVIAITVEDEEDIGKFKDYTPSSTADAAPTKAEPTPAPPKEEKVKQPSSPP 231

Query: 161 VAAAAPVSVAKAAKVAKVVVASTHPASEGGKRIVASPYAKKLAKELNVELATVVGTGPLG 220
                     KA+K       ST P    G R+ ASP A+KLA++ NV L+ + GTGP G
Sbjct: 232 --------EPKASK------PSTPPT---GDRVFASPLARKLAEDNNVPLSDIEGTGPEG 291

Query: 221 RIVAKDV-EAAASAAASSVSAPGGGV-KPAPSLELGTTVPFTTMQGAVSRNMVESLAVPT 280
           RIV  D+ E  AS+   + + P       AP+L+          +   SR       +P 
Sbjct: 292 RIVKADIDEYLASSGKGATAKPSKSTDSKAPALDYVDIPHSQIRKVTASRLAFSKQTIPH 351

Query: 281 FRVGYTITTDALDSLYKKIKS-------KGVTMTALLAKATALALAKHPVVNSSCRDGKS 340
           + +      D L +L  ++ S       K +++  L+ KA ALAL K P  NSS  D   
Sbjct: 352 YYLTVDTCVDKLMALRSQLNSFKEASGGKRISVNDLVVKAAALALRKVPQCNSSWTDDYI 411

Query: 341 FTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLSRKWKELVDKARAKQLQPQEYNTGT 400
             + +++NI VAV  + GL  PV++DAD+  + ++  + + L  KA+   L+P++Y  GT
Sbjct: 412 RQF-KNVNINVAVQTENGLYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPEDYEGGT 471

Query: 401 FTLSNL-GMFGVDRFDAILPPGTGAIMAVGASIPTVV-GTKDGRIGKKNQMQVNVTADHR 460
           FT+SNL G FG+ +F A++ P   AI+AVG++   VV G    +    + M V ++ DHR
Sbjct: 472 FTVSNLGGPFGIKQFCAVVNPPQAAILAVGSAEKRVVPGNGPDQFNFASYMPVTLSCDHR 531

Query: 461 VIYGADLATFLQTLAKIIEDPKDLTL 468
           V+ GA  A +L+     IE+PK + L
Sbjct: 532 VVDGAIGAEWLKAFKGYIENPKSMLL 539

BLAST of Lsi02G019410 vs. TAIR 10
Match: AT1G54220.2 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 199.5 bits (506), Expect = 5.9e-51
Identity = 160/446 (35.87%), Postives = 238/446 (53.36%), Query Frame = 0

Query: 41  REIFMPALSSTMTEGKIVSWIKTEGDKLAKGESVVVVESDKADMDVETFYDGYLAAIMVD 100
           +EI MP+LS TMTEG I  W+K EGDK+A GE +  VE+DKA +++E   +GYLA I+  
Sbjct: 112 QEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKA 171

Query: 101 EGG-VAPVGSAIALLAETEDEISEAK-------SKAAISSASPASAPPPEKSPDNAAAVP 160
           EG     VG  IA+  E E++I + K       + AA + A P  APP E+     ++ P
Sbjct: 172 EGSKEIQVGEVIAITVEDEEDIGKFKDYTPSSTADAAPTKAEPTPAPPKEEKVKQPSSPP 231

Query: 161 VAAAAPVSVAKAAKVAKVVVASTHPASEGGKRIVASPYAKKLAKELNVELATVVGTGPLG 220
                     KA+K       ST P    G R+ ASP A+KLA++ NV L+ + GTGP G
Sbjct: 232 --------EPKASK------PSTPPT---GDRVFASPLARKLAEDNNVPLSDIEGTGPEG 291

Query: 221 RIVAKDV-EAAASAAASSVSAPGGGV-KPAPSLELGTTVPFTTMQGAVSRNMVESLAVPT 280
           RIV  D+ E  AS+   + + P       AP+L+          +   SR       +P 
Sbjct: 292 RIVKADIDEYLASSGKGATAKPSKSTDSKAPALDYVDIPHSQIRKVTASRLAFSKQTIPH 351

Query: 281 FRVGYTITTDALDSLYKKIKS-------KGVTMTALLAKATALALAKHPVVNSSCRDGKS 340
           + +      D L +L  ++ S       K +++  L+ KA ALAL K P  NSS  D   
Sbjct: 352 YYLTVDTCVDKLMALRSQLNSFKEASGGKRISVNDLVVKAAALALRKVPQCNSSWTDDYI 411

Query: 341 FTYNRSINIAVAVAIDGGLITPVLQDADKVDIYSLSRKWKELVDKARAKQLQPQEYNTGT 400
             + +++NI VAV  + GL  PV++DAD+  + ++  + + L  KA+   L+P++Y  GT
Sbjct: 412 RQF-KNVNINVAVQTENGLYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPEDYEGGT 471

Query: 401 FTLSNL-GMFGVDRFDAILPPGTGAIMAVGASIPTVV-GTKDGRIGKKNQMQVNVTADHR 460
           FT+SNL G FG+ +F A++ P   AI+AVG++   VV G    +    + M V ++ DHR
Sbjct: 472 FTVSNLGGPFGIKQFCAVVNPPQAAILAVGSAEKRVVPGNGPDQFNFASYMPVTLSCDHR 531

Query: 461 VIYGADLATFLQTLAKIIEDPKDLTL 468
           V+ GA  A +L+     IE+PK + L
Sbjct: 532 VVDGAIGAEWLKAFKGYIENPKSMLL 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C8P07.0e-18276.39Dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogen... [more]
Q9SQI81.2e-15266.04Dihydrolipoyllysine-residue acetyltransferase component 4 of pyruvate dehydrogen... [more]
Q8RWN91.7e-5036.59Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogen... [more]
Q9R9N32.8e-5035.56Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenas... [more]
Q5M7298.3e-5035.87Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogen... [more]
Match NameE-valueIdentityDescription
A0A0A0K7V14.0e-22593.38Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
A0A5D3CET85.8e-22492.95Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
A0A1S3CG298.4e-22392.52Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
A0A5A7V6B91.4e-22292.75Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
A0A6J1F2173.0e-22090.97Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
Match NameE-valueIdentityDescription
XP_038899200.17.8e-23194.86dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogen... [more]
XP_004150399.18.4e-22593.38dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogen... [more]
TYK09860.11.2e-22392.95dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogen... [more]
XP_008461468.11.7e-22292.52PREDICTED: LOW QUALITY PROTEIN: dihydrolipoyllysine-residue acetyltransferase co... [more]
KAA0061281.13.0e-22292.75dihydrolipoyllysine-residue acetyltransferase component 5 of pyruvate dehydrogen... [more]
Match NameE-valueIdentityDescription
AT1G34430.14.9e-18376.392-oxoacid dehydrogenases acyltransferase family protein [more]
AT3G25860.18.2e-15466.042-oxoacid dehydrogenases acyltransferase family protein [more]
AT3G13930.11.2e-5136.59Dihydrolipoamide acetyltransferase, long form protein [more]
AT1G54220.15.9e-5135.87Dihydrolipoamide acetyltransferase, long form protein [more]
AT1G54220.25.9e-5135.87Dihydrolipoamide acetyltransferase, long form protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004167Peripheral subunit-binding domainPFAMPF02817E3_bindingcoord: 185..220
e-value: 2.3E-14
score: 53.4
IPR004167Peripheral subunit-binding domainPROSITEPS51826PSBDcoord: 186..223
score: 19.376724
IPR0010782-oxoacid dehydrogenase acyltransferase, catalytic domainPFAMPF001982-oxoacid_dhcoord: 247..466
e-value: 1.0E-63
score: 214.9
IPR000089Biotin/lipoyl attachmentPFAMPF00364Biotin_lipoylcoord: 42..113
e-value: 8.4E-16
score: 57.6
IPR000089Biotin/lipoyl attachmentPROSITEPS50968BIOTINYL_LIPOYLcoord: 40..115
score: 27.094486
IPR036625E3-binding domain superfamilyGENE3D4.10.320.10coord: 173..225
e-value: 1.9E-15
score: 58.3
IPR036625E3-binding domain superfamilySUPERFAMILY47005Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complexcoord: 178..223
NoneNo IPR availableGENE3D2.40.50.100coord: 35..139
e-value: 3.2E-26
score: 93.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 125..149
NoneNo IPR availablePANTHERPTHR23151DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATEDcoord: 28..467
NoneNo IPR availablePANTHERPTHR23151:SF75DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT 5 OF PYRUVATE DEHYDROGENASE COMPLEX, CHLOROPLASTICcoord: 28..467
NoneNo IPR availableCDDcd06849lipoyl_domaincoord: 42..114
e-value: 1.77415E-26
score: 99.7863
NoneNo IPR availableSUPERFAMILY52777CoA-dependent acyltransferasescoord: 247..465
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 241..466
e-value: 6.0E-70
score: 237.5
IPR0030162-oxo acid dehydrogenase, lipoyl-binding sitePROSITEPS00189LIPOYLcoord: 65..94
IPR011053Single hybrid motifSUPERFAMILY51230Single hybrid motifcoord: 39..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G019410.1Lsi02G019410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004742 dihydrolipoyllysine-residue acetyltransferase activity
molecular_function GO:0016746 acyltransferase activity