Lsi02G019300 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G019300 vs. ExPASy Swiss-Prot
Match: Q9CZJ9 (DnaJ homolog subfamily C member 18 OS=Mus musculus OX=10090 GN=Dnajc18 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.5e-08
Identity = 34/74 (45.95%), Postives = 45/74 (60.81%), Query Frame = 0

Query: 323 YDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPDKRKALD 382
           YDILG++ N S E +KK Y K++L  HPDK   P A +AF  + NAF  L +PDKR   D
Sbjct: 84  YDILGVSHNASDEELKKAYKKLALKFHPDKNCAPGATEAFKAIGNAFAVLSNPDKRLRYD 143

Query: 383 DKIKFKEEQEQFKV 397
           +   + +EQ  F V
Sbjct: 144 E---YGDEQVTFTV 154

BLAST of Lsi02G019300 vs. ExPASy Swiss-Prot
Match: Q17438 (DnaJ homolog subfamily B member 1 OS=Caenorhabditis elegans OX=6239 GN=dnj-1 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.5e-08
Identity = 31/82 (37.80%), Postives = 47/82 (57.32%), Query Frame = 0

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           T+ + E  E +    D      Y+IL +++  S ++I+K Y K++L +HPDKC  P A +
Sbjct: 122 TSEQKELVERIRHCKDY-----YEILKIDKKASDDDIRKEYRKLALKLHPDKCRAPHATE 181

Query: 361 AFIRLNNAFKELQDPDKRKALD 383
           AF  L NA+  L D DKR+  D
Sbjct: 182 AFKALGNAYAVLSDTDKRRQYD 198

BLAST of Lsi02G019300 vs. ExPASy Swiss-Prot
Match: Q4V893 (GPALPP motifs-containing protein 1 OS=Rattus norvegicus OX=10116 GN=Gpalpp1 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 5.9e-08
Identity = 58/181 (32.04%), Postives = 85/181 (46.96%), Query Frame = 0

Query: 420 ELLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPSDRA 479
           E L   DD+      R+ WMT LPPE K    +    F + + +  GD SVWTDTP+DR 
Sbjct: 181 EKLTKGDDDSPKPVTRESWMTELPPEMK-DFGLGPRTFKRRADDKSGDRSVWTDTPADRE 240

Query: 480 QKAKMSYLEAYNEAAALASNEEEAKKYSSESAL---VDKYNQEKRSKSLVEKHREGAKSR 539
           +KAK    E      +L+  +EE      +  L   V  YN+ KRS+SL++ H +  K++
Sbjct: 241 RKAK----EIQEARKSLSKKDEENMLSGRDKRLAEQVSSYNESKRSESLMDIHHKRLKTK 300

Query: 540 GKKKSKQVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPE------NMAQGLGSRFSS 592
             +   +  E+           P+DR+KDL   R    FD          ++ L +RFS 
Sbjct: 301 AAEDKNRHQER----------TPFDRDKDLKVNR----FDEAQKKALIKKSRELNTRFSH 342

BLAST of Lsi02G019300 vs. ExPASy Swiss-Prot
Match: Q2QUP1 (DnaJ protein ERDJ7 OS=Oryza sativa subsp. japonica OX=39947 GN=ERDJ7 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.0e-07
Identity = 30/80 (37.50%), Postives = 48/80 (60.00%), Query Frame = 0

Query: 311 VSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFK 370
           VS  +  E D  YD+LG+ ++ ++  IKK Y+K+SL  HPDK   P++ + F+++ NA++
Sbjct: 33  VSNAIYCEEDDCYDLLGVKQDANVSEIKKAYYKLSLKHHPDKNPDPESRKLFVKIANAYE 92

Query: 371 ELQDPDKRKALDDKIKFKEE 391
            L+D   R   D  I   EE
Sbjct: 93  ILKDESTRGQYDYAIAHPEE 112

BLAST of Lsi02G019300 vs. ExPASy Swiss-Prot
Match: P56101 (DnaJ homolog subfamily C member 5 OS=Tetronarce californica OX=7787 GN=dnajc5 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.0e-07
Identity = 32/75 (42.67%), Postives = 46/75 (61.33%), Query Frame = 0

Query: 309 EEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDK-CSHPQANQAFIRLNN 368
           ++  R +   GDS Y +LGL++N S E+IKK Y K++L  HPDK   +P+A++ F  +NN
Sbjct: 3   DQRQRSLSTSGDSLYIVLGLDKNASPEDIKKSYRKLALKYHPDKNPDNPEASEKFKEINN 62

Query: 369 AFKELQDPDKRKALD 383
           A   L D  KR   D
Sbjct: 63  AHAILTDATKRNIYD 77

BLAST of Lsi02G019300 vs. ExPASy TrEMBL
Match: A0A1S3CFW3 (uncharacterized protein LOC103500427 OS=Cucumis melo OX=3656 GN=LOC103500427 PE=4 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 1.1e-294
Identity = 557/602 (92.52%), Postives = 582/602 (96.68%), Query Frame = 0

Query: 1   MGSRRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRFRDRER 60
           MGSRRK+ RTV S++ SSSSPSSSDDSDSSSMRSRRH+RS SSRREK+KERESR RDRER
Sbjct: 51  MGSRRKKNRTVGSEDPSSSSPSSSDDSDSSSMRSRRHYRSDSSRREKEKERESRIRDRER 110

Query: 61  RRKDRN----RERDRKRRKKKLRRESKRKRDDYGSESDNESSHSGDSVDSKSDRRKVKII 120
           +RK+RN    RERDRKRRKKKLRRESKRKRDD+GS+SDNESSHSGDSVD+KSDRRKVKI+
Sbjct: 111 KRKNRNRERDRERDRKRRKKKLRRESKRKRDDFGSDSDNESSHSGDSVDTKSDRRKVKIM 170

Query: 121 EPEVIVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQ 180
           EPEVIVRQLLK+FPD+GNDLKQLLKMID GQAVDISGIS +SLTKRLKKLF+SLNLEESQ
Sbjct: 171 EPEVIVRQLLKDFPDIGNDLKQLLKMIDDGQAVDISGISVKSLTKRLKKLFLSLNLEESQ 230

Query: 181 ERVFLLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSE 240
           ERVFLLPS+V PTLEVIGPLIE HM+QKSN VD S+TLDDKSSPPLNMETTQAREDNMSE
Sbjct: 231 ERVFLLPSSVHPTLEVIGPLIEMHMDQKSNPVDDSITLDDKSSPPLNMETTQAREDNMSE 290

Query: 241 LAYPDGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMV 300
           LAYPDGEASGPK+RMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMV
Sbjct: 291 LAYPDGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMV 350

Query: 301 AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP 360
           AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP
Sbjct: 351 AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP 410

Query: 361 QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMD 420
           QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISM+
Sbjct: 411 QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISME 470

Query: 421 GDDELLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPS 480
           GD+ELLANMDDEV  EPKRDEWMT+LPPERKPGMTMQSSRFSKSSKEGRGDTS+WTDTPS
Sbjct: 471 GDEELLANMDDEVTAEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSIWTDTPS 530

Query: 481 DRAQKAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSR 540
           DRAQKAKMSYLEAYNEAAALASN EEAKKYS+ESALVDKYNQEKRSKSLVEKHREG KSR
Sbjct: 531 DRAQKAKMSYLEAYNEAAALASN-EEAKKYSAESALVDKYNQEKRSKSLVEKHREGVKSR 590

Query: 541 GKKKSKQVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRN 599
           GK KSKQVAEKEDDWVGKHPWKPWDREKDLTAGRK+INFDPENMAQGLGSRFSSGSYQRN
Sbjct: 591 GKNKSKQVAEKEDDWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRN 650

BLAST of Lsi02G019300 vs. ExPASy TrEMBL
Match: A0A6J1EHJ8 (DNA ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC111433443 PE=4 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 5.8e-277
Identity = 529/599 (88.31%), Postives = 562/599 (93.82%), Query Frame = 0

Query: 1   MGSRRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRFRDRER 60
           MGS RK+KRT +  E SSSSPSSSD+SDSSSMRSRRH RSGSSRREKDK+RESR RDRER
Sbjct: 1   MGSGRKKKRT-ECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRER 60

Query: 61  RRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESSHSGDSVDSKSDRRKVKIIEPEV 120
           +RKDRNRERDRK RKKKLRRE+KRKRDDYGSESD ESS SG+S DS+ +RRK++ +EPEV
Sbjct: 61  KRKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEV 120

Query: 121 IVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQERVF 180
           +VR+LLKEFPDVGNDLKQLL MID GQAVDISGISER+L KRLKKLF+SLNLEESQERVF
Sbjct: 121 VVRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVF 180

Query: 181 LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSELAYP 240
           LLPSN  PTLE IGPLI+THMEQK NQ DHSVT D+KSSPPLN ETTQ+ +DN+S+L +P
Sbjct: 181 LLPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHP 240

Query: 241 DGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE 300
           DGEA+GPK+RMLGPEMPSAELLAAAAKLTEAQTELRE ELEED ELFVGPPPPAMVAEAE
Sbjct: 241 DGEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAE 300

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           TANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ
Sbjct: 301 TANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360

Query: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDGDDE 420
           AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISM+GDDE
Sbjct: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDE 420

Query: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPSDRAQ 480
           LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSK SKEGRGDTSVWTDTPSDRAQ
Sbjct: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQ 480

Query: 481 KAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKK 540
           KAK SYLEAYNEAAALAS+ EEAKK SSESALVDKYN+EKRSK L+EKHREGA+S GKKK
Sbjct: 481 KAKTSYLEAYNEAAALASS-EEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGKKK 540

Query: 541 SK-QVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 599
           SK QV EKE++WVGKHPWKPWDREKDLTAGRKTIN DPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 SKQQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINLDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of Lsi02G019300 vs. ExPASy TrEMBL
Match: A0A6J1DVD5 (uncharacterized protein LOC111023459 OS=Momordica charantia OX=3673 GN=LOC111023459 PE=4 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 2.8e-263
Identity = 516/598 (86.29%), Postives = 547/598 (91.47%), Query Frame = 0

Query: 1   MGSRRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRFRDRER 60
           MGS RK+ RT DS E SSSSPSS DDSD+S+ RSRRHHR G SRREKDK+RES      R
Sbjct: 1   MGSGRKKWRTDDS-EPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRES------R 60

Query: 61  RRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESSHSGDSVDSKSDRRKVKIIEPEV 120
           +RKDR RERDRKRRKKK RRE KRKR DY SESD+ SS        +S+  KV+ IEPEV
Sbjct: 61  KRKDRMRERDRKRRKKKSRREGKRKR-DYFSESDDGSS-------GESELEKVR-IEPEV 120

Query: 121 IVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQERVF 180
           +VRQLLKEFPDVG+DL+QLLKMID GQAVDISGISERSLTKRLKKLF SLNLEE+Q+RVF
Sbjct: 121 VVRQLLKEFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVF 180

Query: 181 LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSELAYP 240
           LLPSN R TLE+IGP+I+ HMEQK NQ DHSVTLD+KSSPP NMETTQAREDNMSELA P
Sbjct: 181 LLPSNGRSTLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACP 240

Query: 241 DGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE 300
           DGEASGPK+RMLGPEMPSAELLAAAAKLTEAQTELREAELEED+ELFVGPPPPAMVAEAE
Sbjct: 241 DGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAE 300

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ
Sbjct: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360

Query: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDGDDE 420
           AFIRLNNAFKELQDPDKRKALDDKIK KEEQEQFKVELRGMREAAKWR+SQGISMDGD+E
Sbjct: 361 AFIRLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEE 420

Query: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPSDRAQ 480
           LLAN +D+VKVEPKRDEWMT+LPPERKPG+TMQSSRFSKSSKEGRGDTSVWTDTP DRAQ
Sbjct: 421 LLANTEDDVKVEPKRDEWMTTLPPERKPGITMQSSRFSKSSKEGRGDTSVWTDTPLDRAQ 480

Query: 481 KAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKK 540
           KAK SYLEAYNEA ALASN EEAKK+SSESALVDKYN+EKRSKSL+EKHRE  +SR KKK
Sbjct: 481 KAKTSYLEAYNEATALASN-EEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKK 540

Query: 541 SKQVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 599
           SKQVAEK DDWVGKHPWKPWDREKDLTAGRKT+NFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 SKQVAEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581

BLAST of Lsi02G019300 vs. ExPASy TrEMBL
Match: A0A2C9WFD9 (J domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_02G191800 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 4.1e-198
Identity = 408/604 (67.55%), Postives = 485/604 (80.30%), Query Frame = 0

Query: 1   MGSRRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHR-----SGSSRREKDKERESRF 60
           MGS+ ++K   D+  SSS+  S  DDS++S  R RR  +      GSSRRE D++RE   
Sbjct: 1   MGSKTRKKHREDTPTSSSN--SDDDDSETSEDRRRRRRKDRRSSDGSSRRE-DRKRER-- 60

Query: 61  RDRERRRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNES-SHSGDSVDSKSDRRKVK 120
           RD +RR++   +ERDRKRRK        RK+ DY SES++ S S SG   D++  +R  +
Sbjct: 61  RDEKRRKRKERKERDRKRRK-------SRKKRDYDSESESGSGSESGSGSDAEEPKR--Q 120

Query: 121 IIEPEVIVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEE 180
            ++PE +V+++L EFPDVG+DLKQLL+MID GQAVDI GISE+ L K LKKLFVSLNL E
Sbjct: 121 RVKPEDVVKEMLMEFPDVGDDLKQLLQMIDSGQAVDIKGISEKPLIKHLKKLFVSLNLME 180

Query: 181 SQERVFLLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNM 240
             +RVFLLPSN  PTLE++GPLIE H+  K  +VD     D  S PP+  E+ Q  ++N 
Sbjct: 181 KGDRVFLLPSNNCPTLELVGPLIEAHIRSKEQKVD-----DVHSVPPV-AESKQLSDNNN 240

Query: 241 SELAYPDGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPA 300
           S +  PD +ASGP++RM+GPEMPSAELLAAAAKLTEA  ELREAELEEDDELF+GP PPA
Sbjct: 241 SVMPAPDDDASGPRRRMIGPEMPSAELLAAAAKLTEAHAELREAELEEDDELFIGPAPPA 300

Query: 301 MVAEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCS 360
           MVAEAE+ANEAERFEEV+RIM VEGDSPYD++G N N S ENIKKRYWK+SLLVHPDKCS
Sbjct: 301 MVAEAESANEAERFEEVTRIMGVEGDSPYDVVGANHNTSAENIKKRYWKLSLLVHPDKCS 360

Query: 361 HPQANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGIS 420
           HPQA+QAFI+LN AFKELQDPDKRK +D+KIK KEEQEQFKVEL+ MREAA+WR+SQGIS
Sbjct: 361 HPQAHQAFIKLNKAFKELQDPDKRKLMDEKIKLKEEQEQFKVELKAMREAAQWRRSQGIS 420

Query: 421 MDGDDELLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDT 480
           M+GDDELLA  + EVKV PKRDEWMT+LPPERKPGMTM SS+FSK S+EGRGDTSVWTDT
Sbjct: 421 MEGDDELLA--ETEVKVAPKRDEWMTTLPPERKPGMTMHSSKFSKGSREGRGDTSVWTDT 480

Query: 481 PSDRAQKAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAK 540
           PSDRAQKAKM+YLEAYNEAAALASNEEE K+ S+++ LVDKYN+ KRSKSLV+KH+E A 
Sbjct: 481 PSDRAQKAKMNYLEAYNEAAALASNEEEKKRSSADADLVDKYNKAKRSKSLVQKHQEEAA 540

Query: 541 SRGKKKSKQVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQ 599
           SR KKKSK+  EKE +W G+HPWKPWDREKDL AGR+ I FD +NMAQGL SRFSSG++Q
Sbjct: 541 SRSKKKSKEQPEKE-EWAGQHPWKPWDREKDLVAGRQNIKFDADNMAQGLSSRFSSGNFQ 581

BLAST of Lsi02G019300 vs. ExPASy TrEMBL
Match: A0A5D1ZY69 (J domain-containing protein OS=Gossypium darwinii OX=34276 GN=ES288_D13G152000v1 PE=4 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 3.3e-195
Identity = 402/599 (67.11%), Postives = 488/599 (81.47%), Query Frame = 0

Query: 4   RRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRFRDRERRRK 63
           R+ RK+ VD   +SSSSPS+SDDS SS  R  RH +    RR KD   ES  R++ER+R+
Sbjct: 3   RKTRKKHVDDTPTSSSSPSTSDDSKSSLERRHRHRK---VRRRKD---ESSRREKERKRE 62

Query: 64  DRNR-ERDRKRRKKKLRRESKR-KRDDYGSESDNESSHSGDSVDSKSDRRKVKIIEPEVI 123
            R R E++R+R+++K RRE +R KR DYGS+S + S  S  S DS+ D+R+V   EPEV+
Sbjct: 63  KRKRKEKERERKRRKSRREDRREKRRDYGSDSKSGSGSSSGS-DSEEDKRRV---EPEVV 122

Query: 124 VRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQERVFL 183
           V+Q+LKEFP+VGNDLKQLL+MID GQAVDI GISERSL K LKKLF+SL L+E+ +RVFL
Sbjct: 123 VQQILKEFPNVGNDLKQLLQMIDDGQAVDIKGISERSLNKHLKKLFLSLKLKENGDRVFL 182

Query: 184 LPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSELAYPD 243
           L S  RPTL V+G LI+ H E K  Q+ +S ++ D  S P + E++Q  ++N S+     
Sbjct: 183 LSSKSRPTLGVVGHLIQAHTEPKEQQLTNSGSVKDAHSIPEHAESSQVMDENNSD----R 242

Query: 244 GEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAET 303
            +++ PKKR++GP MPSAELLAAAAKLTEAQ ELREAE+EEDDELF+GPPPPA+VAEAE+
Sbjct: 243 DDSATPKKRVIGPAMPSAELLAAAAKLTEAQAELREAEIEEDDELFIGPPPPALVAEAES 302

Query: 304 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 363
           ANEAERFEEV+RIM V  D PYD++G NRN + +NIKK+YWK+SL+VHPDKC HPQA+QA
Sbjct: 303 ANEAERFEEVTRIMGVGSDCPYDVIGANRNTTADNIKKKYWKLSLMVHPDKCPHPQAHQA 362

Query: 364 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDGDDEL 423
           FI LN AFKELQDPDKRKALD+KIK KEEQE+FK ELR MREAA+WR+ QGISM+GDDEL
Sbjct: 363 FIILNKAFKELQDPDKRKALDEKIKLKEEQEEFKAELRAMREAAQWRRLQGISMEGDDEL 422

Query: 424 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPSDRAQK 483
           LA +  EVK  PKRDEWMT+LPPERKPG+TMQS++FSKS+KEGRGDTSVWTDTP +RAQK
Sbjct: 423 LAEV--EVKAPPKRDEWMTTLPPERKPGVTMQSTKFSKSTKEGRGDTSVWTDTPLERAQK 482

Query: 484 AKMSYLEAYNEAAALASNEEE-AKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKK 543
           AKM YLEAYNEAAALASNEEE  K+ SS++ LVDKYN+EKRSKSLV+KH+E A  R KKK
Sbjct: 483 AKMHYLEAYNEAAALASNEEEKTKRSSSDADLVDKYNKEKRSKSLVQKHQEEASKRPKKK 542

Query: 544 SKQVAEKE-DDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 599
           SKQ +EKE D+W GKHPWKPWDREKDLTAGR+++N D ENM +GL SRFS+GS+QRNFL
Sbjct: 543 SKQQSEKEKDEWEGKHPWKPWDREKDLTAGRQSVNLDTENMVKGLTSRFSTGSFQRNFL 585

BLAST of Lsi02G019300 vs. NCBI nr
Match: XP_038898675.1 (DNA ligase 1 [Benincasa hispida])

HSP 1 Score: 1066.2 bits (2756), Expect = 1.0e-307
Identity = 581/598 (97.16%), Postives = 590/598 (98.66%), Query Frame = 0

Query: 1   MGSRRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRFRDRER 60
           MGSRRK+KRTVDSDE SSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESR RDRER
Sbjct: 1   MGSRRKKKRTVDSDEPSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRSRDRER 60

Query: 61  RRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESSHSGDSVDSKSDRRKVKIIEPEV 120
           +RKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNES HSGDSVDS+SDRRKVKIIEPEV
Sbjct: 61  KRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESPHSGDSVDSRSDRRKVKIIEPEV 120

Query: 121 IVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQERVF 180
           IVRQLLKEFPDVGNDLKQLLKMID GQAVDISGISERSL KRLKKLFVSLNLEESQERVF
Sbjct: 121 IVRQLLKEFPDVGNDLKQLLKMIDDGQAVDISGISERSLAKRLKKLFVSLNLEESQERVF 180

Query: 181 LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSELAYP 240
           LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVT+DDKSSPPLNMETTQARED+MSELAYP
Sbjct: 181 LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTVDDKSSPPLNMETTQAREDDMSELAYP 240

Query: 241 DGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE 300
           DGEASGPK+RMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE
Sbjct: 241 DGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE 300

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           TANEAERFEEV+RIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ
Sbjct: 301 TANEAERFEEVTRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360

Query: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDGDDE 420
           AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISM+GD+E
Sbjct: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEE 420

Query: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPSDRAQ 480
           LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTP DRAQ
Sbjct: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPFDRAQ 480

Query: 481 KAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKK 540
           KAKMSYLEAYNEAAALASN EEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKK
Sbjct: 481 KAKMSYLEAYNEAAALASN-EEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKK 540

Query: 541 SKQVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 599
           SKQV EKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 SKQVVEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of Lsi02G019300 vs. NCBI nr
Match: XP_008461948.1 (PREDICTED: uncharacterized protein LOC103500427 [Cucumis melo])

HSP 1 Score: 1021.9 bits (2641), Expect = 2.2e-294
Identity = 557/602 (92.52%), Postives = 582/602 (96.68%), Query Frame = 0

Query: 1   MGSRRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRFRDRER 60
           MGSRRK+ RTV S++ SSSSPSSSDDSDSSSMRSRRH+RS SSRREK+KERESR RDRER
Sbjct: 51  MGSRRKKNRTVGSEDPSSSSPSSSDDSDSSSMRSRRHYRSDSSRREKEKERESRIRDRER 110

Query: 61  RRKDRN----RERDRKRRKKKLRRESKRKRDDYGSESDNESSHSGDSVDSKSDRRKVKII 120
           +RK+RN    RERDRKRRKKKLRRESKRKRDD+GS+SDNESSHSGDSVD+KSDRRKVKI+
Sbjct: 111 KRKNRNRERDRERDRKRRKKKLRRESKRKRDDFGSDSDNESSHSGDSVDTKSDRRKVKIM 170

Query: 121 EPEVIVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQ 180
           EPEVIVRQLLK+FPD+GNDLKQLLKMID GQAVDISGIS +SLTKRLKKLF+SLNLEESQ
Sbjct: 171 EPEVIVRQLLKDFPDIGNDLKQLLKMIDDGQAVDISGISVKSLTKRLKKLFLSLNLEESQ 230

Query: 181 ERVFLLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSE 240
           ERVFLLPS+V PTLEVIGPLIE HM+QKSN VD S+TLDDKSSPPLNMETTQAREDNMSE
Sbjct: 231 ERVFLLPSSVHPTLEVIGPLIEMHMDQKSNPVDDSITLDDKSSPPLNMETTQAREDNMSE 290

Query: 241 LAYPDGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMV 300
           LAYPDGEASGPK+RMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMV
Sbjct: 291 LAYPDGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMV 350

Query: 301 AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP 360
           AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP
Sbjct: 351 AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP 410

Query: 361 QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMD 420
           QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISM+
Sbjct: 411 QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISME 470

Query: 421 GDDELLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPS 480
           GD+ELLANMDDEV  EPKRDEWMT+LPPERKPGMTMQSSRFSKSSKEGRGDTS+WTDTPS
Sbjct: 471 GDEELLANMDDEVTAEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSIWTDTPS 530

Query: 481 DRAQKAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSR 540
           DRAQKAKMSYLEAYNEAAALASN EEAKKYS+ESALVDKYNQEKRSKSLVEKHREG KSR
Sbjct: 531 DRAQKAKMSYLEAYNEAAALASN-EEAKKYSAESALVDKYNQEKRSKSLVEKHREGVKSR 590

Query: 541 GKKKSKQVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRN 599
           GK KSKQVAEKEDDWVGKHPWKPWDREKDLTAGRK+INFDPENMAQGLGSRFSSGSYQRN
Sbjct: 591 GKNKSKQVAEKEDDWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRN 650

BLAST of Lsi02G019300 vs. NCBI nr
Match: XP_004150393.1 (uncharacterized protein LOC101213388 [Cucumis sativus] >KGN44664.2 hypothetical protein Csa_016603 [Cucumis sativus])

HSP 1 Score: 1011.1 bits (2613), Expect = 3.9e-291
Identity = 550/598 (91.97%), Postives = 574/598 (95.99%), Query Frame = 0

Query: 1   MGSRRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRFRDRER 60
           MGSRRK+KRT  S++ SSSSPSSSDDSDSSSMRSRRH+RS SSRREKDKERESR RDRER
Sbjct: 1   MGSRRKKKRTAGSEDPSSSSPSSSDDSDSSSMRSRRHYRSDSSRREKDKERESRIRDRER 60

Query: 61  RRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESSHSGDSVDSKSDRRKVKIIEPEV 120
           +RKDRNRERDRKRRKKKLRRESKRK+DDYGSESDNESSHSG+SV++KSDRRKVKI EPEV
Sbjct: 61  KRKDRNRERDRKRRKKKLRRESKRKKDDYGSESDNESSHSGNSVNTKSDRRKVKIKEPEV 120

Query: 121 IVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQERVF 180
           I+RQLLK+FPD+GNDLKQLLKMID GQAVDISGIS + LTKRLK LFVSLNLEESQERVF
Sbjct: 121 ILRQLLKDFPDIGNDLKQLLKMIDDGQAVDISGISVKCLTKRLKTLFVSLNLEESQERVF 180

Query: 181 LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSELAYP 240
           LLPS+VRPTLEVIGPLIE HM+QKS  VD ++TLDDKSSPPLNME TQAREDNMSELAYP
Sbjct: 181 LLPSSVRPTLEVIGPLIEMHMDQKSKPVDDAITLDDKSSPPLNMEATQAREDNMSELAYP 240

Query: 241 DGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE 300
           DGEASGPK+RMLGPEMPSAELLAAAAKLTEAQTELRE ELEEDDELFVGPPPPAMVAEAE
Sbjct: 241 DGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDDELFVGPPPPAMVAEAE 300

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP ANQ
Sbjct: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPHANQ 360

Query: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDGDDE 420
           AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISM+GD+E
Sbjct: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEE 420

Query: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPSDRAQ 480
           LLANMDDEV  EPKRDEWMT+LPPERKPGMTMQSSRFSKSSKE RGDTSVWTDTPSDRAQ
Sbjct: 421 LLANMDDEVTAEPKRDEWMTNLPPERKPGMTMQSSRFSKSSKESRGDTSVWTDTPSDRAQ 480

Query: 481 KAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKK 540
           KAKMSYLEAYNEAAALASN EEAKKYS+ESALVDKYNQEKRSKSLVEKHREG KSRGKKK
Sbjct: 481 KAKMSYLEAYNEAAALASN-EEAKKYSAESALVDKYNQEKRSKSLVEKHREGVKSRGKKK 540

Query: 541 SKQVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 599
            KQV EKED+WVGKHPWKPWDREKDLTAGRK+INFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 LKQVVEKEDEWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of Lsi02G019300 vs. NCBI nr
Match: XP_022926268.1 (DNA ligase 1 [Cucurbita moschata])

HSP 1 Score: 963.0 bits (2488), Expect = 1.2e-276
Identity = 529/599 (88.31%), Postives = 562/599 (93.82%), Query Frame = 0

Query: 1   MGSRRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRFRDRER 60
           MGS RK+KRT +  E SSSSPSSSD+SDSSSMRSRRH RSGSSRREKDK+RESR RDRER
Sbjct: 1   MGSGRKKKRT-ECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRER 60

Query: 61  RRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESSHSGDSVDSKSDRRKVKIIEPEV 120
           +RKDRNRERDRK RKKKLRRE+KRKRDDYGSESD ESS SG+S DS+ +RRK++ +EPEV
Sbjct: 61  KRKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEV 120

Query: 121 IVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQERVF 180
           +VR+LLKEFPDVGNDLKQLL MID GQAVDISGISER+L KRLKKLF+SLNLEESQERVF
Sbjct: 121 VVRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVF 180

Query: 181 LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSELAYP 240
           LLPSN  PTLE IGPLI+THMEQK NQ DHSVT D+KSSPPLN ETTQ+ +DN+S+L +P
Sbjct: 181 LLPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHP 240

Query: 241 DGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE 300
           DGEA+GPK+RMLGPEMPSAELLAAAAKLTEAQTELRE ELEED ELFVGPPPPAMVAEAE
Sbjct: 241 DGEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAE 300

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           TANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ
Sbjct: 301 TANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360

Query: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDGDDE 420
           AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISM+GDDE
Sbjct: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDE 420

Query: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPSDRAQ 480
           LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSK SKEGRGDTSVWTDTPSDRAQ
Sbjct: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQ 480

Query: 481 KAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKK 540
           KAK SYLEAYNEAAALAS+ EEAKK SSESALVDKYN+EKRSK L+EKHREGA+S GKKK
Sbjct: 481 KAKTSYLEAYNEAAALASS-EEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGKKK 540

Query: 541 SK-QVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 599
           SK QV EKE++WVGKHPWKPWDREKDLTAGRKTIN DPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 SKQQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINLDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of Lsi02G019300 vs. NCBI nr
Match: KAG7011072.1 (hypothetical protein SDJN02_27870, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 962.6 bits (2487), Expect = 1.6e-276
Identity = 531/600 (88.50%), Postives = 563/600 (93.83%), Query Frame = 0

Query: 1   MGSRRKRKRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRFRDRER 60
           MGS RK+KRT +  E SSSSPSSSD+SDSSSMRSRRH RSGSSRREKDK+RESR RDRER
Sbjct: 119 MGSGRKKKRT-ECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRER 178

Query: 61  RRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESSHSGDSVDSKSDRRKVKIIEPEV 120
           +RKDRNRERDRK RKKKLRRE+KRKRDDYGSESD ESS SG+S DS+ +RRK++ +EPEV
Sbjct: 179 KRKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEV 238

Query: 121 IVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQERVF 180
           +VR+LLKEFPDVGNDLKQLL MID GQAVDISGISER+L KRLKKLF+SLNLEESQERVF
Sbjct: 239 VVRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVF 298

Query: 181 LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSELAYP 240
           LLPSN  PTLE IGPLI+THMEQK NQ DHSVT D+KSSPPLN ETTQ+ +DN+S+L +P
Sbjct: 299 LLPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHP 358

Query: 241 DGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE 300
           DGEA+GPK+RMLGPEMPSAELLAAAAKLTEAQTELRE ELEED ELFVGPPPPAMVAEAE
Sbjct: 359 DGEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAE 418

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           TANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ
Sbjct: 419 TANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 478

Query: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDGDDE 420
           AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISM+GDDE
Sbjct: 479 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDE 538

Query: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPSDRAQ 480
           LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSK SKEGRGDTSVWTDTPSDRAQ
Sbjct: 539 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQ 598

Query: 481 KAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSR-GKK 540
           KAK SYLEAYNEAAALASN EEAKK SSESALVDKYN+EKRSK L+EKHREGA+S  GKK
Sbjct: 599 KAKTSYLEAYNEAAALASN-EEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGGKK 658

Query: 541 KSK-QVAEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 599
           KSK QV EKE++WVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 659 KSKQQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 716

BLAST of Lsi02G019300 vs. TAIR 10
Match: AT1G65280.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 583.9 bits (1504), Expect = 1.4e-166
Identity = 358/610 (58.69%), Postives = 452/610 (74.10%), Query Frame = 0

Query: 1   MGSRRKR-KRTVDSDESSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKD---KERESRFR 60
           MG   KR   + D   SS++S SSS  SD S   SR+  +    RR+ D    ERE R R
Sbjct: 1   MGKHWKRDSDSGDDTPSSATSSSSSYSSDESDSSSRKWRKKQKERRKSDGGSYEREKR-R 60

Query: 61  DRERRRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESSHSGDSVDSKSDRRKVKII 120
            RE+ RK +  ER ++R+++ ++++ K K+ +Y  ESD ES    DS   + D       
Sbjct: 61  KREKERKRKKIER-KERKRRDMKKKKKTKKREY--ESDTESYSGSDSFSDQED------- 120

Query: 121 EPEVIVRQLLKEFPDVGNDLKQLLKMIDGGQAVDISGISERSLTKRLKKLFVSLNLEESQ 180
           +PE +V+++L EFP+VGNDLKQLLKMID GQAVDI GISE +L KRLKKLF+SL L+E  
Sbjct: 121 DPETVVKEMLLEFPNVGNDLKQLLKMIDDGQAVDIKGISESTLKKRLKKLFLSLKLKERG 180

Query: 181 ERVFLLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTLDDKSSPPLNMETTQAREDNMSE 240
           +RVFLLP    P+L+V+G LI      K  + D   +LDD SSP    E   A E+ +  
Sbjct: 181 DRVFLLPPGASPSLDVVGHLI------KGGEEDVEKSLDD-SSPLKETEKGLADENGLGS 240

Query: 241 LAYPDGEASGPKKRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMV 300
            +  D   +GPKKR++GP MPSAELLAAAAKLTEAQ ELREAELEED E F+GP PPA+V
Sbjct: 241 NSADD--VTGPKKRVIGPAMPSAELLAAAAKLTEAQAELREAELEEDSEYFIGPAPPAVV 300

Query: 301 AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP 360
           AE  ++NEAERFEEV+RIM+ + +SPYD+LG+N N++ +N+KKRYWK+SLLVHPDKCSHP
Sbjct: 301 AEVASSNEAERFEEVTRIMEADANSPYDVLGVNHNMAADNMKKRYWKLSLLVHPDKCSHP 360

Query: 361 QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMD 420
           QA +AF+ LN AFKELQDP+KRKA+DDKIK KEEQE FKVELR M+EAA+WR+SQGISM+
Sbjct: 361 QAQEAFVLLNKAFKELQDPEKRKAMDDKIKLKEEQEAFKVELRSMQEAAQWRRSQGISME 420

Query: 421 GDDELLANMDDEVKVEPKRDEWMTSLPPERKPGMTM-QSSRFSKSSKEGRGDTSVWTDTP 480
           GD ELLA    EVK EPKRDEWMT+LPPERK G+ + QS+ FS++++EGRGDT+ WTDTP
Sbjct: 421 GDAELLA--ATEVKPEPKRDEWMTTLPPERKVGVAVQQSTTFSRNAREGRGDTTAWTDTP 480

Query: 481 SDRAQKAKMSYLEAYNEAAALASNEEEAKKYSSESALVDKYNQEKRSKSLVEKHRE---G 540
            D+A++AKM+YLEAYN+A+ALASNE E  K S ++ LVDKYN+EKR+KSLVEKHRE    
Sbjct: 481 MDKAERAKMNYLEAYNKASALASNEGENMKRSMDAELVDKYNKEKRAKSLVEKHREDSSS 540

Query: 541 AKSRGKKKSKQVAEKE----DDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRF 599
           + SR KKK K  + KE    D+WVGKHPWKPWDRE DLTAGR+ +  D + MA+GL S+F
Sbjct: 541 SSSRLKKKKKLSSSKEKTEKDEWVGKHPWKPWDRENDLTAGRQNVKLDADGMAEGLASKF 588

BLAST of Lsi02G019300 vs. TAIR 10
Match: AT5G22080.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 8.4e-10
Identity = 41/131 (31.30%), Postives = 74/131 (56.49%), Query Frame = 0

Query: 304 EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI 363
           E ER  EV RI+     +P++ L L+ + S +++K++Y K+SL+VHPDKC HPQA +AF 
Sbjct: 21  EVERDNEVGRILSCFKLNPFEHLNLSFDSSTDDVKRQYRKISLMVHPDKCKHPQAQEAFG 80

Query: 364 RLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDGDDELLA 423
            L  A + L +  +R  +  ++   +E+ + K + +  ++ A   K + +  +G  E + 
Sbjct: 81  ALAKAQQLLLNDQERDYILTQVHAAKEELKMKRKKQLKKDTAS--KIKSLVDEGKHEHIY 140

Query: 424 NMDDEVKVEPK 435
              +E + E K
Sbjct: 141 EQSEEFQKELK 149

BLAST of Lsi02G019300 vs. TAIR 10
Match: AT5G22080.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 8.4e-10
Identity = 41/131 (31.30%), Postives = 74/131 (56.49%), Query Frame = 0

Query: 304 EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI 363
           E ER  EV RI+     +P++ L L+ + S +++K++Y K+SL+VHPDKC HPQA +AF 
Sbjct: 21  EVERDNEVGRILSCFKLNPFEHLNLSFDSSTDDVKRQYRKISLMVHPDKCKHPQAQEAFG 80

Query: 364 RLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDGDDELLA 423
            L  A + L +  +R  +  ++   +E+ + K + +  ++ A   K + +  +G  E + 
Sbjct: 81  ALAKAQQLLLNDQERDYILTQVHAAKEELKMKRKKQLKKDTAS--KIKSLVDEGKHEHIY 140

Query: 424 NMDDEVKVEPK 435
              +E + E K
Sbjct: 141 EQSEEFQKELK 149

BLAST of Lsi02G019300 vs. TAIR 10
Match: AT5G49580.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 3.0e-07
Identity = 47/159 (29.56%), Postives = 79/159 (49.69%), Query Frame = 0

Query: 267 KLTEAQTELREAELEEDDEL-----FVGPPPPAMVAEAETANEAERFEEVSRIMDVEGDS 326
           K+ +  T  R  E   D        F G   P + + + T +E    +E++R+++     
Sbjct: 351 KINQQSTADRPTEPVNDSSFENGPGFPGDRGPGVASTSGTDSELTSEDEIARLLNC--PD 410

Query: 327 PYDILGLNR--NLSIENIKKRYWKMSLLVHPDK-CSHPQANQAFIRLNNAFKELQDPDKR 386
            Y  LGL R  N+ +  +K+ Y K ++LVHPDK   + +A +AF +L NA++ L D  K+
Sbjct: 411 HYSALGLARYGNVDMAYLKREYRKKAMLVHPDKNMGNERAAEAFKKLQNAYEVLLDSVKQ 470

Query: 387 KALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMDG 418
           K+ DD++K +E    F+   R    + K  +  G S  G
Sbjct: 471 KSYDDELKREELLNYFR---RFQNSSQKDTRGHGFSGSG 504