Lsi02G018950 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G018950
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Glutathione transport system permease gsiD
Location	chr02: 24871161 .. 24872008 (+)
RNA-Seq Expression	Lsi02G018950
Synteny	Lsi02G018950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAAACGTAGACCGTTATCATAATTCAGGAAGCCCTTAAAAATGGGGTAGCAAACTATTCTCCATCACTCCGCCGGTAAAATCCTTTTTCACGATGAATTGCATTTCCTCCTCCATTGAATTGCCTCTTCGCCGATCGCATCTCAAGCCAAATCGACGGCAACGGCAATGGCAACAGAATTCACCGCCGAAGAATCTCCACGTCTTTCGCCACATTTACATTTCTCCACAAACCTACCGATTCGATTCATTAAAAGCCAATGCAATTCTTCCAACCTCTTTAATTCTCGGAAACAGCACTATTCCTCCGGCGCAGTCTGGCGACGTTTCGGTCCTTCTCCAGACAGGGTATGCCAATTTTCTCCTCTGTTTAAATTCGAATCGATGCACAAATTACTTAGAACCGATCTGAGATTAATCTCTTCAATTTTAATTCTTTTTTTCTTTCGGCAATTTGTGCAGCGGCGTGCTTCTGATTGCGTATTTGATAGCGAATTTCATTCTGCCGGAGTTTATCATGAAATCGTACCGATCCGAAGAGAGCGACGGAAAAGCTGCGGTGGAAGATGAAGAATCAAGCGAAGGGAGGAAGAAGCAAGGCTTTGGCGGCAGAGGGAGATAACGAAACTTGAAGGTGAAGCCATTAGTTTTATTTTATTTTATTATTATTATTATTATTTTTTTTTTTGGTGTTGTATAGGGAAATTGAGATAAGGTATTTGATTCGCTGTGATTTTCTTTTGCCACGTGACTGAAAAGGAAATTTCTGTAGTTTACCCTTTATTTTTGAAATCTGTCTGACATATTTGTGGAAAATAGAATCAAATAGTGAATGGAGTTCGATTTTTC

mRNA sequence

CCAAACGTAGACCGTTATCATAATTCAGGAAGCCCTTAAAAATGGGGTAGCAAACTATTCTCCATCACTCCGCCGGTAAAATCCTTTTTCACGATGAATTGCATTTCCTCCTCCATTGAATTGCCTCTTCGCCGATCGCATCTCAAGCCAAATCGACGGCAACGGCAATGGCAACAGAATTCACCGCCGAAGAATCTCCACGTCTTTCGCCACATTTACATTTCTCCACAAACCTACCGATTCGATTCATTAAAAGCCAATGCAATTCTTCCAACCTCTTTAATTCTCGGAAACAGCACTATTCCTCCGGCGCAGTCTGGCGACGTTTCGGTCCTTCTCCAGACAGGCGGCGTGCTTCTGATTGCGTATTTGATAGCGAATTTCATTCTGCCGGAGTTTATCATGAAATCGTACCGATCCGAAGAGAGCGACGGAAAAGCTGCGGTGGAAGATGAAGAATCAAGCGAAGGGAGGAAGAAGCAAGGCTTTGGCGGCAGAGGGAGATAACGAAACTTGAAGGTGAAGCCATTAGTTTTATTTTATTTTATTATTATTATTATTATTTTTTTTTTTGGTGTTGTATAGGGAAATTGAGATAAGGTATTTGATTCGCTGTGATTTTCTTTTGCCACGTGACTGAAAAGGAAATTTCTGTAGTTTACCCTTTATTTTTGAAATCTGTCTGACATATTTGTGGAAAATAGAATCAAATAGTGAATGGAGTTCGATTTTTC

Coding sequence (CDS)

ATGAATTGCATTTCCTCCTCCATTGAATTGCCTCTTCGCCGATCGCATCTCAAGCCAAATCGACGGCAACGGCAATGGCAACAGAATTCACCGCCGAAGAATCTCCACGTCTTTCGCCACATTTACATTTCTCCACAAACCTACCGATTCGATTCATTAAAAGCCAATGCAATTCTTCCAACCTCTTTAATTCTCGGAAACAGCACTATTCCTCCGGCGCAGTCTGGCGACGTTTCGGTCCTTCTCCAGACAGGCGGCGTGCTTCTGATTGCGTATTTGATAGCGAATTTCATTCTGCCGGAGTTTATCATGAAATCGTACCGATCCGAAGAGAGCGACGGAAAAGCTGCGGTGGAAGATGAAGAATCAAGCGAAGGGAGGAAGAAGCAAGGCTTTGGCGGCAGAGGGAGATAA

Protein sequence

MNCISSSIELPLRRSHLKPNRRQRQWQQNSPPKNLHVFRHIYISPQTYRFDSLKANAILPTSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEESDGKAAVEDEESSEGRKKQGFGGRGR

Homology

[Close]

BLAST of Lsi02G018950 vs. ExPASy TrEMBL
Match: A0A6J1FK57 (uncharacterized protein LOC111446047 OS=Cucurbita moschata OX=3662 GN=LOC111446047 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 7.1e-37
Identity = 96/139 (69.06%), Postives = 111/139 (79.86%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQWQQNSPPKNLHVFRHIYISPQTYRFDSLKANAILP 60
           MNCI+S+IELP+ RSH KPNRRQR+ Q NS  +       IYISPQ+ RF SL ANAI P
Sbjct: 18  MNCITSAIELPVLRSHFKPNRRQRK-QNNSQSQ-----PRIYISPQSNRFASLTANAI-P 77

Query: 61  TSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEESDGKAAVED 120
            SL+L NSTIPP+QSGDVSVLLQT GVLLI YLIANFI+PEFIMKSY ++ES+GKA +ED
Sbjct: 78  NSLVLANSTIPPSQSGDVSVLLQTSGVLLIVYLIANFIVPEFIMKSYSTDESEGKALMED 137

Query: 121 EE--SSEGRKKQGFGGRGR 138
           +E  +SE RKKQGFGG  R
Sbjct: 138 DEKGASERRKKQGFGGNKR 149

[Close]

BLAST of Lsi02G018950 vs. ExPASy TrEMBL
Match: A0A6J1J030 (uncharacterized protein LOC111480699 OS=Cucurbita maxima OX=3661 GN=LOC111480699 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.9e-36
Identity = 96/141 (68.09%), Postives = 110/141 (78.01%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQW--QQNSPPKNLHVFRHIYISPQTYRFDSLKANAI 60
           MNCI+S+IELP+ RSH KPNRRQR+    Q+ PP        IYISPQ+ RF SL ANAI
Sbjct: 27  MNCITSAIELPVLRSHFKPNRRQRKQNNSQSQPP--------IYISPQSNRFASLTANAI 86

Query: 61  LPTSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEESDGKAAV 120
            P SL+L N TIPP+QSGDVSVLLQT GVLLI YLIANFI+PEFIMKSY ++ES+GKA +
Sbjct: 87  -PNSLVLAN-TIPPSQSGDVSVLLQTSGVLLIVYLIANFIVPEFIMKSYSTDESEGKALM 146

Query: 121 EDEE--SSEGRKKQGFGGRGR 138
           EDEE  +SE RKKQGFGG  R
Sbjct: 147 EDEEIGASERRKKQGFGGNKR 157

[Close]

BLAST of Lsi02G018950 vs. ExPASy TrEMBL
Match: A0A5D3BI04 (Glutathione transport system permease gsiD OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold27427G00010 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.0e-27
Identity = 81/118 (68.64%), Postives = 90/118 (76.27%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQWQQNSPPKNLHVFRHIYISPQ-TYRFDSLKANAIL 60
           MN I SSIE  L  S   PN  Q       P  NLH+F  I++SPQ TYRF+S+K N+IL
Sbjct: 1   MNSIFSSIEFRLLPS---PNPPQ-------PQHNLHIFHQIHLSPQTTYRFNSIKTNSIL 60

Query: 61  PT-SLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRS-EESDGK 116
           PT SLILGNSTIPPAQSGD+SVLLQT GVLLIAYLIANFI PEFIMKSYRS +ES+GK
Sbjct: 61  PTNSLILGNSTIPPAQSGDISVLLQTSGVLLIAYLIANFIFPEFIMKSYRSDDESEGK 108

[Close]

BLAST of Lsi02G018950 vs. ExPASy TrEMBL
Match: A0A0A0K9M2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420170 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 5.1e-27
Identity = 76/117 (64.96%), Postives = 87/117 (74.36%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQWQQNSPPKNLHVFRHIYISPQ-TYRFDSLKANAIL 60
           MN I SSI+  L  S   PN RQ       P  NLH+F  IY+ PQ T+RF S+K N+IL
Sbjct: 1   MNSIFSSIQFRLLPS---PNPRQ-------PQHNLHIFHQIYLPPQITHRFSSIKTNSIL 60

Query: 61  PTSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRS-EESDGK 116
           PTSLILGNSTIPP+QSGD+SVLL   GVLLIAYL+ANFI PEFIMKSYRS +E+DGK
Sbjct: 61  PTSLILGNSTIPPSQSGDISVLLPISGVLLIAYLLANFIFPEFIMKSYRSDDENDGK 107

[Close]

BLAST of Lsi02G018950 vs. ExPASy TrEMBL
Match: A0A6I9U4E6 (uncharacterized protein LOC105174880 OS=Sesamum indicum OX=4182 GN=LOC105174880 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 6.9e-08
Identity = 43/123 (34.96%), Postives = 72/123 (58.54%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQWQQNSPPKNLHVFRHIYISPQTYRFDSLKANAILP 60
           M CI+S  +L    SH   ++ + Q+ ++ P         + ++P  + F SL+  ++ P
Sbjct: 1   MKCINSQSQLSPLPSH---HKYKHQFCESKPSS-------MRLNPNGFSFVSLRTRSLPP 60

Query: 61  TSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEES--DGKAAV 120
           + + L  ++  PA +G++SVLLQT  V L AY +ANF++PE I+K  RS+E+  DG+   
Sbjct: 61  SPIFLAATS--PAAAGNLSVLLQTSAVCLFAYFVANFVVPELILKDLRSKEANKDGENPA 111

Query: 121 EDE 122
           EDE
Sbjct: 121 EDE 111

[Close]

BLAST of Lsi02G018950 vs. NCBI nr
Match: XP_038897194.1 (uncharacterized protein LOC120085329 [Benincasa hispida])

HSP 1 Score: 221.9 bits (564), Expect = 3.5e-54
Identity = 122/138 (88.41%), Postives = 126/138 (91.30%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQWQQNSPP-KNLHVFRHIYISPQTYRFDSLKANAIL 60
           MNCISSSIE P  RSHLKPNRRQ   QQNSPP KNLH+FRHIYISPQTYRFDSLK NAI 
Sbjct: 1   MNCISSSIEFPPLRSHLKPNRRQP--QQNSPPKKNLHIFRHIYISPQTYRFDSLKVNAI- 60

Query: 61  PTSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEESDGKAAVE 120
           P+SLILGNSTIPP+QSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRS+ESDGKAA E
Sbjct: 61  PSSLILGNSTIPPSQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSDESDGKAAAE 120

Query: 121 DEESSEGRKKQGFGGRGR 138
           DEESSEGRKKQGF GR R
Sbjct: 121 DEESSEGRKKQGFSGRRR 135

[Close]

BLAST of Lsi02G018950 vs. NCBI nr
Match: XP_023525256.1 (uncharacterized protein LOC111788910 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 165.2 bits (417), Expect = 3.9e-37
Identity = 97/139 (69.78%), Postives = 111/139 (79.86%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQWQQNSPPKNLHVFRHIYISPQTYRFDSLKANAILP 60
           MNCI+S+IELP+ RSHLKPNRRQRQ Q NS  +       +YISPQ+ RF SL ANAI P
Sbjct: 18  MNCITSAIELPILRSHLKPNRRQRQ-QNNSQSQ-----PRMYISPQSNRFASLTANAI-P 77

Query: 61  TSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEESDGKAAVED 120
            SL+L NSTIPP+QSGDVSVLLQT GVLLI Y IANFI+PEFIMKSY ++ES+GKA +ED
Sbjct: 78  NSLVLANSTIPPSQSGDVSVLLQTSGVLLIVYFIANFIVPEFIMKSYSTDESEGKALMED 137

Query: 121 EE--SSEGRKKQGFGGRGR 138
           EE  +SE RKKQGFGG  R
Sbjct: 138 EEKGASERRKKQGFGGNKR 149

[Close]

BLAST of Lsi02G018950 vs. NCBI nr
Match: XP_022940449.1 (uncharacterized protein LOC111446047 [Cucurbita moschata] >KAG7037370.1 hypothetical protein SDJN02_00995 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 163.3 bits (412), Expect = 1.5e-36
Identity = 96/139 (69.06%), Postives = 111/139 (79.86%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQWQQNSPPKNLHVFRHIYISPQTYRFDSLKANAILP 60
           MNCI+S+IELP+ RSH KPNRRQR+ Q NS  +       IYISPQ+ RF SL ANAI P
Sbjct: 18  MNCITSAIELPVLRSHFKPNRRQRK-QNNSQSQ-----PRIYISPQSNRFASLTANAI-P 77

Query: 61  TSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEESDGKAAVED 120
            SL+L NSTIPP+QSGDVSVLLQT GVLLI YLIANFI+PEFIMKSY ++ES+GKA +ED
Sbjct: 78  NSLVLANSTIPPSQSGDVSVLLQTSGVLLIVYLIANFIVPEFIMKSYSTDESEGKALMED 137

Query: 121 EE--SSEGRKKQGFGGRGR 138
           +E  +SE RKKQGFGG  R
Sbjct: 138 DEKGASERRKKQGFGGNKR 149

[Close]

BLAST of Lsi02G018950 vs. NCBI nr
Match: KAG6585878.1 (hypothetical protein SDJN03_18611, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 162.9 bits (411), Expect = 1.9e-36
Identity = 96/139 (69.06%), Postives = 111/139 (79.86%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQWQQNSPPKNLHVFRHIYISPQTYRFDSLKANAILP 60
           MNCI+S+IELP+ RSH KPNRRQR+ Q NS  +       IYISPQ+ RF SL ANAI P
Sbjct: 18  MNCITSAIELPVLRSHFKPNRRQRK-QNNSQSQ-----PRIYISPQSNRFASLTANAI-P 77

Query: 61  TSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEESDGKAAVED 120
            SL+L NSTIPP+QSGDVSVLLQT GVLLI YLIANFI+PEFIMKSY ++ES+GKA +ED
Sbjct: 78  NSLVLANSTIPPSQSGDVSVLLQTSGVLLIVYLIANFIVPEFIMKSYSTDESEGKALMED 137

Query: 121 EE--SSEGRKKQGFGGRGR 138
           +E  +SE RKKQGFGG  R
Sbjct: 138 DEKGASERRKKQGFGGEFR 149

[Close]

BLAST of Lsi02G018950 vs. NCBI nr
Match: KAG6607795.1 (hypothetical protein SDJN03_01137, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 162.9 bits (411), Expect = 1.9e-36
Identity = 96/139 (69.06%), Postives = 111/139 (79.86%), Query Frame = 0


Query: 1   MNCISSSIELPLRRSHLKPNRRQRQWQQNSPPKNLHVFRHIYISPQTYRFDSLKANAILP 60
           MNCI+S+IELP+ RSH KPNRRQR+ Q NS  +       IYISPQ+ RF SL ANAI P
Sbjct: 18  MNCITSAIELPVLRSHFKPNRRQRK-QNNSQSQ-----PRIYISPQSNRFASLTANAI-P 77

Query: 61  TSLILGNSTIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEESDGKAAVED 120
            SL+L NSTIPP+QSGDVSVLLQT GVLLI YLIANFI+PEFIMKSY ++ES+GKA +ED
Sbjct: 78  NSLVLANSTIPPSQSGDVSVLLQTSGVLLIVYLIANFIVPEFIMKSYSTDESEGKALMED 137

Query: 121 EE--SSEGRKKQGFGGRGR 138
           +E  +SE RKKQGFGG  R
Sbjct: 138 DEKGASERRKKQGFGGEFR 149

[Close]

BLAST of Lsi02G018950 vs. TAIR 10
Match: AT1G50732.1 (unknown protein. )

HSP 1 Score: 45.1 bits (105), Expect = 5.4e-05
Identity = 21/58 (36.21%), Postives = 35/58 (60.34%), Query Frame = 0


Query: 68  STIPPAQSGDVSVLLQTGGVLLIAYLIANFILPEFIMKSYRSEESDGKAAVEDEESSE 126
           S +PP++SGD++  L   G ++  YL+ NF++P  + KS + EE       E+EE S+
Sbjct: 67  SVLPPSESGDITTFLFVSGAMISMYLVTNFLVPSLLFKSLQGEEE------EEEEDSD 118

The following BLAST results are available for this feature:

BLAST of Lsi02G018950 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi02G018950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FK57	7.1e-37	69.06	uncharacterized protein LOC111446047 OS=Cucurbita moschata OX=3662 GN=LOC1114460...	[more]
A0A6J1J030	7.9e-36	68.09	uncharacterized protein LOC111480699 OS=Cucurbita maxima OX=3661 GN=LOC111480699...	[more]
A0A5D3BI04	1.0e-27	68.64	Glutathione transport system permease gsiD OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A0A0K9M2	5.1e-27	64.96	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420170 PE=4 SV=1	[more]
A0A6I9U4E6	6.9e-08	34.96	uncharacterized protein LOC105174880 OS=Sesamum indicum OX=4182 GN=LOC105174880 ...	[more]

BLAST of Lsi02G018950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897194.1	3.5e-54	88.41	uncharacterized protein LOC120085329 [Benincasa hispida]	[more]
XP_023525256.1	3.9e-37	69.78	uncharacterized protein LOC111788910 [Cucurbita pepo subsp. pepo]	[more]
XP_022940449.1	1.5e-36	69.06	uncharacterized protein LOC111446047 [Cucurbita moschata] >KAG7037370.1 hypothet...	[more]
KAG6585878.1	1.9e-36	69.06	hypothetical protein SDJN03_18611, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6607795.1	1.9e-36	69.06	hypothetical protein SDJN03_01137, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lsi02G018950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G50732.1	5.4e-05	36.21	unknown protein.	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 113..137
None	No IPR available	PANTHER	PTHR37196	TRANSMEMBRANE PROTEIN	coord: 1..125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G018950.1	Lsi02G018950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane