Lsi02G017750 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G017750
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Calcium-binding EF-hand family protein
Location	chr02: 23534449 .. 23534925 (-)
RNA-Seq Expression	Lsi02G017750
Synteny	Lsi02G017750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCCATTGAACTCCTTCGATTTGCAGAGAATTTTCGAAACGATAGACAGAAATGGCGATGGCTTCGTGAGCATGGAGGAGCTCAATTGGCTGCTTAATCGTGTAGGCGTGGACCTAATTTCGGAGGATCTGGAGTCATTAATTGGTAAATCTAAGATTGATTTGAATGAATTTTTGTGTCTGTACGAATCGATAAGGGAGAAAAACGAGGGTGATAACAGGGGAAATGAGGAGGAGATTGAGAGCGATCTTTGGAATGCATTCGAAGTGTTCGACGAAAATCGTGATGGTTTCATTTCGTGCGATGAGCTTCAGAATGTGTTGGTGAGATTGGGGCTTTGGAATGATGAACGCTGTGATGGCGGTGGTGATTATTGTAGGCGTATGATCGGAGCGTTTGATATGAATTTGGATGGGCGGCTTGATTTTCAGGAATTCAAGAACATGATGAACTCTTCCAATTCTTCCTCTTGA

mRNA sequence

ATGTCTCCATTGAACTCCTTCGATTTGCAGAGAATTTTCGAAACGATAGACAGAAATGGCGATGGCTTCGTGAGCATGGAGGAGCTCAATTGGCTGCTTAATCGTGTAGGCGTGGACCTAATTTCGGAGGATCTGGAGTCATTAATTGGTAAATCTAAGATTGATTTGAATGAATTTTTGTGTCTGTACGAATCGATAAGGGAGAAAAACGAGGGTGATAACAGGGGAAATGAGGAGGAGATTGAGAGCGATCTTTGGAATGCATTCGAAGTGTTCGACGAAAATCGTGATGGTTTCATTTCGTGCGATGAGCTTCAGAATGTGTTGGTGAGATTGGGGCTTTGGAATGATGAACGCTGTGATGGCGGTGGTGATTATTGTAGGCGTATGATCGGAGCGTTTGATATGAATTTGGATGGGCGGCTTGATTTTCAGGAATTCAAGAACATGATGAACTCTTCCAATTCTTCCTCTTGA

Coding sequence (CDS)

ATGTCTCCATTGAACTCCTTCGATTTGCAGAGAATTTTCGAAACGATAGACAGAAATGGCGATGGCTTCGTGAGCATGGAGGAGCTCAATTGGCTGCTTAATCGTGTAGGCGTGGACCTAATTTCGGAGGATCTGGAGTCATTAATTGGTAAATCTAAGATTGATTTGAATGAATTTTTGTGTCTGTACGAATCGATAAGGGAGAAAAACGAGGGTGATAACAGGGGAAATGAGGAGGAGATTGAGAGCGATCTTTGGAATGCATTCGAAGTGTTCGACGAAAATCGTGATGGTTTCATTTCGTGCGATGAGCTTCAGAATGTGTTGGTGAGATTGGGGCTTTGGAATGATGAACGCTGTGATGGCGGTGGTGATTATTGTAGGCGTATGATCGGAGCGTTTGATATGAATTTGGATGGGCGGCTTGATTTTCAGGAATTCAAGAACATGATGAACTCTTCCAATTCTTCCTCTTGA

Protein sequence

MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFLCLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS

Homology

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BLAST of Lsi02G017750 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 4.5e-24
Identity = 60/147 (40.82%), Postives = 95/147 (64.63%), Query Frame = 0


Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVG-VDLISEDLESLIGKSKIDLNEFLCLYESI 67
           DL+R+F+T+D+N DG V+++EL W+L+++G  +   ++LE ++GK  +DL+EFL  Y   
Sbjct: 10  DLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRFYYDA 69

Query: 68  REKNEGDNRGNEEEIESD--LWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCDGGG 127
              ++G  +  +   ++D  +  AF VFD N DG+IS +EL++VL RLG   + +     
Sbjct: 70  VLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFEEEAKAWD-- 129

Query: 128 DYCRRMIGAFDMNLDGRLDFQEFKNMM 152
             C RMI   D NLDG +DF+EFKNM+
Sbjct: 130 --CGRMIRVHDKNLDGFVDFEEFKNMI 152

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BLAST of Lsi02G017750 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 7.7e-24
Identity = 70/167 (41.92%), Postives = 101/167 (60.48%), Query Frame = 0


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  LQRIF+  D+NGDGF+++EEL+  L R+G++    DL+S +      G + ++ +
Sbjct: 25  LNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFD 84

Query: 64  EFLCLYESIREKNEG---------DNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNV 123
           +F  L++++ +   G         D+  +  E ESDL  AF+VFDEN DGFIS  ELQ V
Sbjct: 85  DFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTV 144

Query: 124 LVRLGLWNDERCDGGGDYCR--RMIGAFDMNLDGRLDFQEFKNMMNS 154
           L +LGL        GG+  R  +MI + D N DGR+DF EFKNMM +
Sbjct: 145 LKKLGL------PEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRT 185

[Close]

BLAST of Lsi02G017750 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.5e-22
Identity = 63/154 (40.91%), Postives = 88/154 (57.14%), Query Frame = 0


Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLNEFLC 67
           +L R+FE  DRNGDG ++ EEL   L ++G+ + +++L ++I      G   +D+ EF  
Sbjct: 92  ELARVFELFDRNGDGRITREELEDSLGKLGIPVPADELAAVIARIDANGDGCVDVEEFGE 151

Query: 68  LYESIR----EKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWND 127
           LY SI     +  +G  +  EEE + D+  AF VFD N DG+I+ DEL  VL  LGL   
Sbjct: 152 LYRSIMAGGDDSKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQG 211

Query: 128 ERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
                  + CRRMIG  D + DGR+DF EF  MM
Sbjct: 212 RT----AEECRRMIGQVDRDGDGRVDFHEFLQMM 241

[Close]

BLAST of Lsi02G017750 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 9.4e-22
Identity = 60/154 (38.96%), Postives = 90/154 (58.44%), Query Frame = 0


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           ++  +L RIF+  DRNGDG ++ +ELN  L  +G+ +  +DL  +I      G   +D+ 
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 64  EFLCLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWND 123
           EF  LY++I E         E + E D+  AF VFD+NRDGFI+ +EL++VL  LGL   
Sbjct: 61  EFGGLYQTIME---------ERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQG 120

Query: 124 ERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
              +     C+RMI   D++ DG ++F+EFK MM
Sbjct: 121 RTLED----CKRMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Lsi02G017750 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.6e-21
Identity = 64/157 (40.76%), Postives = 94/157 (59.87%), Query Frame = 0


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  L R+F+  D+N DGF+++EEL+  L+R+G+D    DL+S +       K+ +  +
Sbjct: 24  LNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFD 83

Query: 64  EFLCLYESIREK-NEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWN 123
           +F  L++++ E    G+    +   ESDL  AF VFDE+ DGFIS  ELQ VL +LGL  
Sbjct: 84  DFAALHKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPE 143

Query: 124 DERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNS 154
                G  +   +MI + D N DGR+DF EFKNMM +
Sbjct: 144 ----AGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQT 176

[Close]

BLAST of Lsi02G017750 vs. ExPASy TrEMBL
Match: A0A6J1IKW9 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111476552 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 4.9e-58
Identity = 121/158 (76.58%), Postives = 133/158 (84.18%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSP N+ DLQRIFETIDRNGDGFVS+EEL+WLLNR GVD IS+DLESLIGKSKID +EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSKIDFDEFL 60

Query: 61  CLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERC 120
            LYESI E N G      EEIESDL NAF+VFDENRDGFISCDEL NVLVRLGLW+D+RC
Sbjct: 61  SLYESISEINRGG-----EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDKRC 120

Query: 121 DGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS 159
                 CRRMIGAFD++LDGRLDF+EFKNMMN S+ SS
Sbjct: 121 VRD---CRRMIGAFDVDLDGRLDFEEFKNMMNYSSHSS 150

[Close]

BLAST of Lsi02G017750 vs. ExPASy TrEMBL
Match: A0A6J1FD73 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111443045 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.6e-56
Identity = 119/158 (75.32%), Postives = 131/158 (82.91%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSP N+ DLQRIFETIDRNGDGFVS+EEL+WLLNR GVD IS+DLESLIGKS ID +EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 61  CLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERC 120
            LYESI E N G      EEIESDL NAF+VFDENRDGFISCDEL NVLVRL LW+D RC
Sbjct: 61  SLYESISEINRGG-----EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLELWDDRRC 120

Query: 121 DGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS 159
                 CRRMIGAFD++LDGRLDF+EFKNMMN S++SS
Sbjct: 121 VRD---CRRMIGAFDVDLDGRLDFEEFKNMMNYSSNSS 150

[Close]

BLAST of Lsi02G017750 vs. ExPASy TrEMBL
Match: A0A6J1CD20 (probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC111010552 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.3e-47
Identity = 105/157 (66.88%), Postives = 123/157 (78.34%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSPL + DLQRIFET+D NGDGF+S+EELN LL+ +G   I +DLESLIGK K+D  EFL
Sbjct: 7   MSPLTASDLQRIFETLDSNGDGFLSVEELNRLLDSIGDGQICDDLESLIGKCKLDFEEFL 66

Query: 61  CLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERC 120
            LY+SI E+N     G EEEIESDL NAF+VFD+NRDGFI C+EL+NVL RLGLW DERC
Sbjct: 67  SLYKSISERNRA---GAEEEIESDLLNAFQVFDQNRDGFICCEELRNVLARLGLW-DERC 126

Query: 121 DGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSS 158
              G  C  MIGAFD N DGRLDFQEFKN+M S+++S
Sbjct: 127 ---GRDCSSMIGAFDTNFDGRLDFQEFKNIMLSASNS 156

[Close]

BLAST of Lsi02G017750 vs. ExPASy TrEMBL
Match: A0A2N9GA67 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24170 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 5.0e-42
Identity = 93/156 (59.62%), Postives = 116/156 (74.36%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLIS-EDLESLIGKSKIDLNEF 60
           MSP+N+ DL RIFE +D+NGDG VS+EELNWLL ++GV +IS E+LES++GKS +DLNEF
Sbjct: 1   MSPINTNDLHRIFEKLDKNGDGLVSLEELNWLLEKIGVQIISQEELESIVGKSSLDLNEF 60

Query: 61  LCLYESIREKNEGDN----RGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLW 120
           L  ++SI ++N   +       EE  ESDL  AF+VFD N DGFISCDELQNVL RLGLW
Sbjct: 61  LSFHDSISKQNSSCSNEVVEEEEENEESDLVKAFKVFDLNGDGFISCDELQNVLSRLGLW 120

Query: 121 NDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           +    +  G  CR MI  +D NLDG+LDF+EFKNMM
Sbjct: 121 D----ENSGRDCRSMIHVYDTNLDGQLDFEEFKNMM 152

[Close]

BLAST of Lsi02G017750 vs. ExPASy TrEMBL
Match: A0A7N2RAY7 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.4e-39
Identity = 89/162 (54.94%), Postives = 109/162 (67.28%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MS + + DLQRIFE +D+NGDG V++EELNWLL ++GV +  ++LESL+GKS +DLNEF 
Sbjct: 1   MSLIKTNDLQRIFEKLDKNGDGLVTLEELNWLLEKIGVQISLDELESLVGKSSLDLNEFF 60

Query: 61  CLYESIREKNEGDNRGNEEEI-----------ESDLWNAFEVFDENRDGFISCDELQNVL 120
             Y SI  K    +R    E+           ESDL  AFEVFD N DGFISCDELQ VL
Sbjct: 61  SFYGSISSKQNNTSRSTSNEVVVVEEEEEENEESDLVKAFEVFDLNGDGFISCDELQKVL 120

Query: 121 VRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
            RLGLW+    +  G  CR MI  +D NLDG+LDF+EFKNMM
Sbjct: 121 SRLGLWD----ENSGKDCRVMIHVYDTNLDGQLDFEEFKNMM 158

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: XP_038899375.1 (calmodulin-like protein 3 [Benincasa hispida])

HSP 1 Score: 277.3 bits (708), Expect = 8.0e-71
Identity = 140/159 (88.05%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSPLNS DLQRIFETIDRNGDGFVSMEELN LLNR+GVDL SEDLESL GKSKIDLNEFL
Sbjct: 56  MSPLNSSDLQRIFETIDRNGDGFVSMEELNLLLNRIGVDLASEDLESLTGKSKIDLNEFL 115

Query: 61  CLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERC 120
           CLYESI EK EG+NRG+ EE+ESDL NAF VFDENRDGFISCDEL+NVLVRLGLWNDERC
Sbjct: 116 CLYESISEKKEGENRGS-EEVESDLLNAFMVFDENRDGFISCDELENVLVRLGLWNDERC 175

Query: 121 DG-GGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS 159
            G GGDYCRRMIGAFDM+LDGR+DFQEFKNMMNSSNS S
Sbjct: 176 GGCGGDYCRRMIGAFDMDLDGRIDFQEFKNMMNSSNSPS 213

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: XP_022976028.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 1.0e-57
Identity = 121/158 (76.58%), Postives = 133/158 (84.18%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSP N+ DLQRIFETIDRNGDGFVS+EEL+WLLNR GVD IS+DLESLIGKSKID +EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSKIDFDEFL 60

Query: 61  CLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERC 120
            LYESI E N G      EEIESDL NAF+VFDENRDGFISCDEL NVLVRLGLW+D+RC
Sbjct: 61  SLYESISEINRGG-----EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDKRC 120

Query: 121 DGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS 159
                 CRRMIGAFD++LDGRLDF+EFKNMMN S+ SS
Sbjct: 121 VRD---CRRMIGAFDVDLDGRLDFEEFKNMMNYSSHSS 150

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: KAG7025009.1 (Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 233.4 bits (594), Expect = 1.3e-57
Identity = 123/159 (77.36%), Postives = 133/159 (83.65%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSP N+ DLQRIFETIDRNGDGFVS+EEL+WLLNR GVD IS+DLESLIGKS ID +EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 61  CLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERC 120
            LYESI E N G      EEIESDL NAF+VFDENRDGFISCDEL NVLVRLGLW+D RC
Sbjct: 61  SLYESISEMNRGG-----EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDRRC 120

Query: 121 DGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMN-SSNSSS 159
                 CRRMIGAFD++LDGRLDF+EFKNMMN SSNSSS
Sbjct: 121 VRD---CRRMIGAFDVDLDGRLDFEEFKNMMNYSSNSSS 151

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: XP_023536413.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 231.9 bits (590), Expect = 3.9e-57
Identity = 120/158 (75.95%), Postives = 132/158 (83.54%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSP N+ DLQRIFETIDRNGDGFVS+EEL+WLLNR GVD IS+DLESLIGKS ID +EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 61  CLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERC 120
            LYESI E N G      EEIESDL NAF+VFDENRDGFISCDEL NVLVRLGLW+D RC
Sbjct: 61  SLYESISEINRGG-----EEIESDLLNAFQVFDENRDGFISCDELHNVLVRLGLWDDRRC 120

Query: 121 DGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS 159
                 CRRMIGAFD++LDGRLDF+EFKNMMN S++SS
Sbjct: 121 VRD---CRRMIGAFDVDLDGRLDFEEFKNMMNYSSNSS 150

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: KAG6592136.1 (Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 231.9 bits (590), Expect = 3.9e-57
Identity = 122/159 (76.73%), Postives = 133/159 (83.65%), Query Frame = 0


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSP N+ DLQRIFETIDRNGDGFVS+EEL+WLLNR GVD IS+DLESLIGKS ID +EFL
Sbjct: 1   MSPFNASDLQRIFETIDRNGDGFVSIEELHWLLNRTGVDFISDDLESLIGKSMIDFDEFL 60

Query: 61  CLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERC 120
            LYESI E N G      EEIESDL NAF+VFDEN DGFISCDEL NVLVRLGLW+D+RC
Sbjct: 61  SLYESISEMNRGG-----EEIESDLLNAFQVFDENHDGFISCDELHNVLVRLGLWDDKRC 120

Query: 121 DGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMN-SSNSSS 159
                 CRRMIGAFD++LDGRLDF+EFKNMMN SSNSSS
Sbjct: 121 VRD---CRRMIGAFDVDLDGRLDFEEFKNMMNYSSNSSS 151

[Close]

BLAST of Lsi02G017750 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 112.5 bits (280), Expect = 3.2e-25
Identity = 60/147 (40.82%), Postives = 95/147 (64.63%), Query Frame = 0


Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVG-VDLISEDLESLIGKSKIDLNEFLCLYESI 67
           DL+R+F+T+D+N DG V+++EL W+L+++G  +   ++LE ++GK  +DL+EFL  Y   
Sbjct: 10  DLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRFYYDA 69

Query: 68  REKNEGDNRGNEEEIESD--LWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCDGGG 127
              ++G  +  +   ++D  +  AF VFD N DG+IS +EL++VL RLG   + +     
Sbjct: 70  VLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFEEEAKAWD-- 129

Query: 128 DYCRRMIGAFDMNLDGRLDFQEFKNMM 152
             C RMI   D NLDG +DF+EFKNM+
Sbjct: 130 --CGRMIRVHDKNLDGFVDFEEFKNMI 152

[Close]

BLAST of Lsi02G017750 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 111.7 bits (278), Expect = 5.4e-25
Identity = 70/167 (41.92%), Postives = 101/167 (60.48%), Query Frame = 0


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  LQRIF+  D+NGDGF+++EEL+  L R+G++    DL+S +      G + ++ +
Sbjct: 25  LNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFD 84

Query: 64  EFLCLYESIREKNEG---------DNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNV 123
           +F  L++++ +   G         D+  +  E ESDL  AF+VFDEN DGFIS  ELQ V
Sbjct: 85  DFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTV 144

Query: 124 LVRLGLWNDERCDGGGDYCR--RMIGAFDMNLDGRLDFQEFKNMMNS 154
           L +LGL        GG+  R  +MI + D N DGR+DF EFKNMM +
Sbjct: 145 LKKLGL------PEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRT 185

[Close]

BLAST of Lsi02G017750 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 104.8 bits (260), Expect = 6.7e-23
Identity = 60/154 (38.96%), Postives = 90/154 (58.44%), Query Frame = 0


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           ++  +L RIF+  DRNGDG ++ +ELN  L  +G+ +  +DL  +I      G   +D+ 
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 64  EFLCLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWND 123
           EF  LY++I E         E + E D+  AF VFD+NRDGFI+ +EL++VL  LGL   
Sbjct: 61  EFGGLYQTIME---------ERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQG 120

Query: 124 ERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
              +     C+RMI   D++ DG ++F+EFK MM
Sbjct: 121 RTLED----CKRMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Lsi02G017750 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.1e-22
Identity = 59/157 (37.58%), Postives = 94/157 (59.87%), Query Frame = 0


Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLNEFLC 67
           +L+R+F+  D+NGDG ++ EELN  L  +G+ +  +DL  +I      G   +D++EF  
Sbjct: 65  ELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFES 124

Query: 68  LYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCD 127
           LY SI +++  D    EE    D+ +AF VFD++ DGFI+ +EL++V+  LGL   +  D
Sbjct: 125 LYSSIVDEHHNDGETEEE----DMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLD 184

Query: 128 GGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS 159
           G    C++MI   D + DGR++++EF  MM     SS
Sbjct: 185 G----CKKMIMQVDADGDGRVNYKEFLQMMKGGGFSS 213

[Close]

BLAST of Lsi02G017750 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 104.0 bits (258), Expect = 1.1e-22
Identity = 64/157 (40.76%), Postives = 94/157 (59.87%), Query Frame = 0


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  L R+F+  D+N DGF+++EEL+  L+R+G+D    DL+S +       K+ +  +
Sbjct: 24  LNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFD 83

Query: 64  EFLCLYESIREK-NEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWN 123
           +F  L++++ E    G+    +   ESDL  AF VFDE+ DGFIS  ELQ VL +LGL  
Sbjct: 84  DFAALHKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPE 143

Query: 124 DERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNS 154
                G  +   +MI + D N DGR+DF EFKNMM +
Sbjct: 144 ----AGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQT 176

The following BLAST results are available for this feature:

BLAST of Lsi02G017750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPK5	4.5e-24	40.82	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
Q9SVG9	7.7e-24	41.92	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q0JC44	5.5e-22	40.91	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRR7	9.4e-22	38.96	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9FI19	1.6e-21	40.76	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]

BLAST of Lsi02G017750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IKW9	4.9e-58	76.58	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114765...	[more]
A0A6J1FD73	1.6e-56	75.32	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1CD20	1.3e-47	66.88	probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A2N9GA67	5.0e-42	59.62	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24170 PE=4 SV=1	[more]
A0A7N2RAY7	1.4e-39	54.94	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]

BLAST of Lsi02G017750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899375.1	8.0e-71	88.05	calmodulin-like protein 3 [Benincasa hispida]	[more]
XP_022976028.1	1.0e-57	76.58	probable calcium-binding protein CML44 [Cucurbita maxima]	[more]
KAG7025009.1	1.3e-57	77.36	Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_023536413.1	3.9e-57	75.95	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]
KAG6592136.1	3.9e-57	76.73	Calcium-binding protein CML42, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi02G017750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21550.1	3.2e-25	40.82	Calcium-binding EF-hand family protein	[more]
AT4G20780.1	5.4e-25	41.92	calmodulin like 42	[more]
AT3G07490.1	6.7e-23	38.96	ARF-GAP domain 11	[more]
AT2G43290.1	1.1e-22	37.58	Calcium-binding EF-hand family protein	[more]
AT5G44460.1	1.1e-22	40.76	calmodulin like 43	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 126..154 e-value: 0.04 score: 23.0 coord: 8..36 e-value: 1.6E-4 score: 31.0 coord: 84..112 e-value: 0.11 score: 21.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 84..151 e-value: 4.8E-8 score: 33.3
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 9..29 e-value: 5.2E-6 score: 25.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 122..157 score: 10.329096
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 80..115 score: 14.206556
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 4..39 score: 13.034948
None	No IPR available	GENE3D	1.10.238.10		coord: 71..157 e-value: 6.5E-18 score: 66.9
None	No IPR available	GENE3D	1.10.238.10		coord: 7..70 e-value: 2.0E-9 score: 39.4
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..156
None	No IPR available	PANTHER	PTHR10891:SF788	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 1..156
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 93..105
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 17..29
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 8..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G017750.1	Lsi02G017750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding