Lsi02G017100 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G017100
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Transcription initiation factor IIF subunit beta
Location	chr02: 22835277 .. 22838228 (+)
RNA-Seq Expression	Lsi02G017100
Synteny	Lsi02G017100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCACTATGGAATTGGCTGGCACTGACTCTGGAAATGCATTAAGGTCCTACTCCTTGAATATGTCCACAGACTTCATTCCAATGTCTGTATTTTCCGAGTCATCACAAGGTTGGTATATGTCACTCTAGCCTGCTTATTAAAAATGACCCATGAATATGATGTTTCAAATGTTTGCTTGTAAGCTGGATGAACTATTACTCTGATTGTGATCTGAGATTGAATTTATATTATGTGTTCAACTGGTATTGACATCTTAGTCAATCTTGGATGTGAACAACTACTTTTATTGACTTAGACCATATGTGCATTAATAATTAATTTCAGAATTTGTCTGATTCAATATTAATTATTAATTTTATAGTAATAACATCATGAGTTTGATCATTAATAACACTTACGGACTGTTTGAGCCCCCAACTTGAAGTTGGTGGGGTGGGCTATTATAGCTCACTCCATGTTTGGAGCTCCTAATATTATAGTGGTGTTTACAACTATTTATAACCTATAATTAACTATAGTAATAATATTTGAAATTCTTCTTTGTTATATTGTTTACTATTTCCTACCAATTCTCTCTCTCCCTCTTTGTTACAGTGTTACTATTTCCTACCCAAACTAAAATAGTATGCACCCTAAACATAGACTATTATAATCCATAGGCTATAATAATCATCACGGACTAAAATAACCAGCTCAATGCCCCAAACGCCCCCTCAGTTTGGTGCTATTATCTCTTTTGAATAGAGGTTTGGGCATGGGAGATATCTTAATACACTACTTGCTGGATATTTATATAGTGTGTTTATTGTTTAAAAAAAAAGAAATTTTTTTTTAACGTGGGAGTTAGTATGAGAGACTGTGTTCTGTTGAACCCGACTCCAACGAGTTTATTAGGCTTATGCTGTAATTTATAATTAGGTGAGATGAAGCTTTTGTGCTTAACATTGCCTTCCAAGTTCCATCCCTTTCTATGTTCTTTCTTTCATTAATCCTCTTTAATTCCTTTTCTGTCCAACATCTGACGTGTCAAGACTCAGTTAGGTACCTTGCAATTTTTTGAGTCTTTTATAAACTAATTGTATCTGAAAGTTGTTAACGTGGACTACTGATCTCATACTGATAAATAGTATAACTATATCTAAGGAAAACTAAAAAATCTTTGCAGCAGCTAGAGATGGGGTAATTGATTTCGATTCACATAGCCACTTACTGATCAAATTCTGCAGAGACATTACGTATTTGCATATTAACAAGAGTTCTTAATCATTTGCTATCATGACACATTTATAAGTTCTTTCCGGTATTTTGCGCTGAGGTTCTCTAATGTTAATAGAGGAGATTTCTATTAATCGAGTTATAGTATAGAGAACTCCTATTCAAATACCAGCATGATCTTTCATCTTGAAATTTTCTACTTTGTACATGACAAACAATAAAACTGATAAGTTCTGTGTACTAGAGCTTGACTGCAAGAAAACTATAGCAGCTATAACTTCTATTTTTCATCCTGAAGTTTTATAATTATTTACAGGATAAACATATATAACTAATAAGTTCTGTGGGTGTACTGAAAATACATCAAATTCTTCTTTCATTTTAGGGAAGTTTACGGTCGAAGGGAAAATACTTAACAAATTTGACATGAAGCCACATGATCAGAATCTTGAGAGGTACGGGAAACTCTGTCGTGAAAGAACTCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTCTCCTTTGTAACTTCTTACGCAGTGTACTTGGGTGTTTGGTTACTTATTTATTGTTCTCACGATCTTCTGGTTAAAATTTCCAAATATTCCATACAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCTATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGTAATTATACTACCTTCATTTCTACTTTGTTTGATATCACAATTTTGATTTCACCTGTGCTATATTTAAATATCCTCGTAGTCAGTTAGAAGTTTGTCCAAATGCTTCCAAGAATTATTTTTTACTGTTGTGAACATGTGTGATGCTAATTTTGGTCCAGGAGAAGAAGAAAGTAGTGACCAAGGGATCGGAAACAAAGAGGTTGAGAAAGGAGCGTGGTGAATTGGAGAAGATTATTTTTAGGCTTTTTGAAAGACAGGCATATTGGACGTCAAAACAGTTGATTCAAGAAACTGACCAACCTGAAGTAAGATTTATCTTGAATTTTTTTTTTCCCTCATCACAAAAGGAATACAGCCTAGATTCATTAGGGTGAAAAATTCTCTCCCTCAGATTAAACTTTGAGCTAGGCTATAATTACAAAAAAATTTCTTGTGTCTTTAGACACCACAAGGAAGAGTTCGATTGTACGCCTATCGAATACTAAGCTTGCACATATAGTTTAGCAGCTGGTTTTTTCTGTTTATATAGAATTGAAACCACATTGGGAAATACAAATATAGATAAATGAAACTGTCGTTTTATGCCAACTAGCTTTTAACTTGCGACTAGAGTCTTACATTGTGATCGACTTGAACATAATTTTGGAATATAAAATTAATAGTACTTTATATCCACTCCCCTATTTTTCAGTTAGAAATAGGTCATACATCTCTCAAACATCTGCATCCTTAGGAAAGATGCAAATGAACTGCTCACTTGAAATGCCGCAGTTCAGTTGATAGCTTGATTCTGCTTCTTGTTAGGCTACCACTCGCTAACTATGGATATGATGCCGGAAATAGACTGATTTTATATCACCTTTCAATTCAAATTAGTAAAAGCAATGTTGATTGAAGGCTGAAACATCTAATCTTTCTTTTTTGTAGCAATATATGAAGGAAATACTTAAAGACCTCTGTGTCTACAACAACAAAGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAATACAAGGAATCATGTGAGGAAACGAAACTGCGATAG

mRNA sequence

TTCACTATGGAATTGGCTGGCACTGACTCTGGAAATGCATTAAGGTCCTACTCCTTGAATATGTCCACAGACTTCATTCCAATGTCTGTATTTTCCGAGTCATCACAAGAGACATTACGGAAGTTTACGGTCGAAGGGAAAATACTTAACAAATTTGACATGAAGCCACATGATCAGAATCTTGAGAGGTACGGGAAACTCTGTCGTGAAAGAACTCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTCTCCTTTGTAACTTCTTACGCAGTGTACTTGGGTGTTTGGTTACTTATTTATTGTTCTCACGATCTTCTGGTTAAAATTTCCAAATATTCCATACAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCTATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGAGAAGAAGAAAGTAGTGACCAAGGGATCGGAAACAAAGAGGTTGAGAAAGGAGCGTGGTGAATTGGAGAAGATTATTTTTAGGCTTTTTGAAAGACAGGCATATTGGACGTCAAAACAGTTGATTCAAGAAACTGACCAACCTGAACAATATATGAAGGAAATACTTAAAGACCTCTGTGTCTACAACAACAAAGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAATACAAGGAATCATGTGAGGAAACGAAACTGCGATAG

Coding sequence (CDS)

TTCACTATGGAATTGGCTGGCACTGACTCTGGAAATGCATTAAGGTCCTACTCCTTGAATATGTCCACAGACTTCATTCCAATGTCTGTATTTTCCGAGTCATCACAAGAGACATTACGGAAGTTTACGGTCGAAGGGAAAATACTTAACAAATTTGACATGAAGCCACATGATCAGAATCTTGAGAGGTACGGGAAACTCTGTCGTGAAAGAACTCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTCTCCTTTGTAACTTCTTACGCAGTGTACTTGGGTGTTTGGTTACTTATTTATTGTTCTCACGATCTTCTGGTTAAAATTTCCAAATATTCCATACAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCTATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGAGAAGAAGAAAGTAGTGACCAAGGGATCGGAAACAAAGAGGTTGAGAAAGGAGCGTGGTGAATTGGAGAAGATTATTTTTAGGCTTTTTGAAAGACAGGCATATTGGACGTCAAAACAGTTGATTCAAGAAACTGACCAACCTGAACAATATATGAAGGAAATACTTAAAGACCTCTGTGTCTACAACAACAAAGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAATACAAGGAATCATGTGAGGAAACGAAACTGCGATAG

Protein sequence

FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR

Homology

[Close]

BLAST of Lsi02G017100 vs. ExPASy Swiss-Prot
Match: Q03123 (General transcription factor IIF subunit 2 OS=Xenopus laevis OX=8355 GN=gtf2f2 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-10
Identity = 33/79 (41.77%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 148 KGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGV 207
           K  + KR R ++ ++  ++F  FE+  Y+  K L+  T QP  Y+KEIL+D+ +YN KG 
Sbjct: 184 KKDDGKRARVDKHQVLDMLFSAFEKHQYYNIKDLVDITKQPVTYLKEILRDIGIYNMKGT 243

Query: 208 HQGTYELKPEYKESCEETK 227
           H+ T+ELKPEY+    E K
Sbjct: 244 HKNTWELKPEYRHYQGEDK 262

[Close]

BLAST of Lsi02G017100 vs. ExPASy Swiss-Prot
Match: Q2T9L9 (General transcription factor IIF subunit 2 OS=Bos taurus OX=9913 GN=GTF2F2 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 55/200 (27.50%), Postives = 95/200 (47.50%), Query Frame = 0


Query: 28  MSVFSESSQETLRKFTVEGKILNKFDMKP-HDQNLERYGKLCRERTHKSMTKSRQIQVSF 87
           ++VF+ESS +   K ++EG ++ + + +P  ++N  R  +L  E + K +  S+Q+    
Sbjct: 93  LTVFTESSSD---KLSLEGIVVQRAECRPAANENYMRLKRLQIEESSKPVRLSQQLDKVV 152

Query: 88  VTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 147
            T+Y                 V   +Y+I+                        E+K   
Sbjct: 153 TTNYKP---------------VANHQYNIEY-----------------------ERK--- 212

Query: 148 TKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 207
            K  + KR R ++  +  ++F  FE+  Y+  K L+  T QP  Y+K+ILK++ V N KG
Sbjct: 213 -KKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVSYLKDILKEIGVQNVKG 247

Query: 208 VHQGTYELKPEYKESCEETK 227
           +H+ T+ELKPEY+    E K
Sbjct: 273 IHKNTWELKPEYRHYQVEEK 247

[Close]

BLAST of Lsi02G017100 vs. ExPASy Swiss-Prot
Match: P13984 (General transcription factor IIF subunit 2 OS=Homo sapiens OX=9606 GN=GTF2F2 PE=1 SV=2)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 56/200 (28.00%), Postives = 94/200 (47.00%), Query Frame = 0


Query: 28  MSVFSESSQETLRKFTVEGKILNKFDMKP-HDQNLERYGKLCRERTHKSMTKSRQIQVSF 87
           ++VF+ESS +   K ++EG ++ + + +P   +N  R  +L  E + K +  S+Q+    
Sbjct: 93  LTVFTESSSD---KLSLEGIVVQRAECRPAASENYMRLKRLQIEESSKPVRLSQQLDKVV 152

Query: 88  VTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 147
            T+Y                 V   +Y+I+                        E+K   
Sbjct: 153 TTNYKP---------------VANHQYNIEY-----------------------ERK--- 212

Query: 148 TKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 207
            K  + KR R ++  +  ++F  FE+  Y+  K L+  T QP  Y+KEILK++ V N KG
Sbjct: 213 -KKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKG 247

Query: 208 VHQGTYELKPEYKESCEETK 227
           +H+ T+ELKPEY+    E K
Sbjct: 273 IHKNTWELKPEYRHYQGEEK 247

[Close]

BLAST of Lsi02G017100 vs. ExPASy Swiss-Prot
Match: Q8R0A0 (General transcription factor IIF subunit 2 OS=Mus musculus OX=10090 GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.1e-10
Identity = 54/200 (27.00%), Postives = 94/200 (47.00%), Query Frame = 0


Query: 28  MSVFSESSQETLRKFTVEGKILNKFDMKP-HDQNLERYGKLCRERTHKSMTKSRQIQVSF 87
           ++VF+ESS +   K ++EG ++ + + +P   +N  +  +L  E + K +  S+Q+    
Sbjct: 93  LTVFTESSSD---KLSLEGIVVQRAECRPAASENYMKLKRLQIEESSKPVRLSQQLDKVV 152

Query: 88  VTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 147
            T+Y                 V   +Y+I+                        E+K   
Sbjct: 153 TTNYKP---------------VANHQYNIEY-----------------------ERK--- 212

Query: 148 TKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 207
            K  + KR R ++  +  ++F  FE+  Y+  K L+  T QP  Y+KEILK++ + N KG
Sbjct: 213 -KKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVGYLKEILKEIGIQNVKG 247

Query: 208 VHQGTYELKPEYKESCEETK 227
           +H+ T+ELKPEY+    E K
Sbjct: 273 IHKNTWELKPEYRHYQTEEK 247

[Close]

BLAST of Lsi02G017100 vs. ExPASy Swiss-Prot
Match: P41900 (General transcription factor IIF subunit 2 OS=Drosophila melanogaster OX=7227 GN=TfIIFbeta PE=2 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 7.0e-10
Identity = 32/79 (40.51%), Postives = 49/79 (62.03%), Query Frame = 0


Query: 148 KGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGV 207
           K +E K+ R ++  +  ++F  FE+  Y+  K L++ T+QP  Y+KEILKD+C YN K  
Sbjct: 186 KKAEGKKARDDKNAVMDMLFHAFEKHQYYNIKDLVKITNQPISYLKEILKDVCDYNMKNP 245

Query: 208 HQGTYELKPEYKESCEETK 227
           H+  +ELK EY+    E K
Sbjct: 246 HKNMWELKKEYRHYKTEEK 264

[Close]

BLAST of Lsi02G017100 vs. ExPASy TrEMBL
Match: A0A1S3CPE2 (Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC103503304 PE=3 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.6e-92
Identity = 185/231 (80.09%), Postives = 190/231 (82.25%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQET---LRKFTVEGKILNKFDMKPH 60
           FTMELA TDSGNALRSY+LNMSTDFIPMSVFSESSQ++   L KFTVEGKILNKFDMKPH
Sbjct: 86  FTMELASTDSGNALRSYTLNMSTDFIPMSVFSESSQDSSFVLGKFTVEGKILNKFDMKPH 145

Query: 61  DQNLERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQV 120
           DQNLERYGKLCRERTHKSMTKSRQ                                 IQV
Sbjct: 146 DQNLERYGKLCRERTHKSMTKSRQ---------------------------------IQV 205

Query: 121 IDHVTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWT 180
           IDHVTGGHMRPMPGMDVLSFGAAEKKKVV+KGSETKRLRKERGELEKIIF+LFERQ YWT
Sbjct: 206 IDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWT 265

Query: 181 SKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR 229
           SKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKES EETK R
Sbjct: 266 SKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESSEETKPR 283

[Close]

BLAST of Lsi02G017100 vs. ExPASy TrEMBL
Match: A0A1S3CPB8 (Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC103503304 PE=3 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.7e-91
Identity = 184/228 (80.70%), Postives = 187/228 (82.02%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           FTMELA TDSGNALRSY+LNMSTDFIPMSVFSESSQ    KFTVEGKILNKFDMKPHDQN
Sbjct: 86  FTMELASTDSGNALRSYTLNMSTDFIPMSVFSESSQ---GKFTVEGKILNKFDMKPHDQN 145

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           LERYGKLCRERTHKSMTKSRQ                                 IQVIDH
Sbjct: 146 LERYGKLCRERTHKSMTKSRQ---------------------------------IQVIDH 205

Query: 121 VTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQ 180
           VTGGHMRPMPGMDVLSFGAAEKKKVV+KGSETKRLRKERGELEKIIF+LFERQ YWTSKQ
Sbjct: 206 VTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQ 265

Query: 181 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR 229
           LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKES EETK R
Sbjct: 266 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESSEETKPR 277

[Close]

BLAST of Lsi02G017100 vs. ExPASy TrEMBL
Match: A0A6J1BZ89 (Transcription initiation factor IIF subunit beta OS=Momordica charantia OX=3673 GN=LOC111006053 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.2e-90
Identity = 182/228 (79.82%), Postives = 185/228 (81.14%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           FTMELAGTDSGN+LRSYSLNMSTDFIPMSVFSES      KFTVEGKILNKFDMKPHDQN
Sbjct: 87  FTMELAGTDSGNSLRSYSLNMSTDFIPMSVFSESPP---GKFTVEGKILNKFDMKPHDQN 146

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           LERYGKLCRERTHKSMTKSRQ                                 IQVIDH
Sbjct: 147 LERYGKLCRERTHKSMTKSRQ---------------------------------IQVIDH 206

Query: 121 VTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQ 180
           VTGGHMRPMPGMDV SFGAAEKKKVV+KGSETKRLRKERGELEKIIFRLFERQ YWTSKQ
Sbjct: 207 VTGGHMRPMPGMDVTSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQPYWTSKQ 266

Query: 181 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR 229
           LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKES +ETK R
Sbjct: 267 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESGDETKSR 278

[Close]

BLAST of Lsi02G017100 vs. ExPASy TrEMBL
Match: A0A6J1JIQ3 (Transcription initiation factor IIF subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111487292 PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 7.1e-90
Identity = 180/228 (78.95%), Postives = 185/228 (81.14%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           FTMELA TDSG ALR+YSLNMSTDFIPMSVFSES+Q    KFTVEGKILNKFDMKPHDQN
Sbjct: 85  FTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ---GKFTVEGKILNKFDMKPHDQN 144

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           L+ YGKLCRERTHKSMTKSRQ                                 IQVIDH
Sbjct: 145 LDSYGKLCRERTHKSMTKSRQ---------------------------------IQVIDH 204

Query: 121 VTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQ 180
           VTGGHMRPMPGMDVLSFGAAEKKKVV+KGSE KRLRKERGELEKIIFRLFERQ YWTSKQ
Sbjct: 205 VTGGHMRPMPGMDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQ 264

Query: 181 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR 229
           LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKE+CEETK R
Sbjct: 265 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 276

[Close]

BLAST of Lsi02G017100 vs. ExPASy TrEMBL
Match: A0A6J1HK34 (Transcription initiation factor IIF subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111464830 PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 7.1e-90
Identity = 180/228 (78.95%), Postives = 185/228 (81.14%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           FTMELA TDSG ALR+YSLNMSTDFIPMSVFSES+Q    KFTVEGKILNKFDMKPHDQN
Sbjct: 85  FTMELADTDSGKALRAYSLNMSTDFIPMSVFSESAQ---GKFTVEGKILNKFDMKPHDQN 144

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           L+ YGKLCRERTHKSMTKSRQ                                 IQVIDH
Sbjct: 145 LDSYGKLCRERTHKSMTKSRQ---------------------------------IQVIDH 204

Query: 121 VTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQ 180
           VTGGHMRPMPGMDVLSFGAAEKKKVV+KGSE KRLRKERGELEKIIFRLFERQ YWTSKQ
Sbjct: 205 VTGGHMRPMPGMDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRLFERQPYWTSKQ 264

Query: 181 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR 229
           LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKE+CEETK R
Sbjct: 265 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKETCEETKPR 276

[Close]

BLAST of Lsi02G017100 vs. NCBI nr
Match: XP_008465664.1 (PREDICTED: general transcription factor IIF subunit 2 isoform X1 [Cucumis melo])

HSP 1 Score: 348.2 bits (892), Expect = 5.4e-92
Identity = 185/231 (80.09%), Postives = 190/231 (82.25%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQET---LRKFTVEGKILNKFDMKPH 60
           FTMELA TDSGNALRSY+LNMSTDFIPMSVFSESSQ++   L KFTVEGKILNKFDMKPH
Sbjct: 86  FTMELASTDSGNALRSYTLNMSTDFIPMSVFSESSQDSSFVLGKFTVEGKILNKFDMKPH 145

Query: 61  DQNLERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQV 120
           DQNLERYGKLCRERTHKSMTKSRQ                                 IQV
Sbjct: 146 DQNLERYGKLCRERTHKSMTKSRQ---------------------------------IQV 205

Query: 121 IDHVTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWT 180
           IDHVTGGHMRPMPGMDVLSFGAAEKKKVV+KGSETKRLRKERGELEKIIF+LFERQ YWT
Sbjct: 206 IDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWT 265

Query: 181 SKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR 229
           SKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKES EETK R
Sbjct: 266 SKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESSEETKPR 283

[Close]

BLAST of Lsi02G017100 vs. NCBI nr
Match: XP_038889594.1 (general transcription factor IIF subunit 2-like [Benincasa hispida] >XP_038889595.1 general transcription factor IIF subunit 2-like [Benincasa hispida] >XP_038889596.1 general transcription factor IIF subunit 2-like [Benincasa hispida])

HSP 1 Score: 345.9 bits (886), Expect = 2.7e-91
Identity = 184/226 (81.42%), Postives = 186/226 (82.30%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           FTMELAGTDSGN LRSYSLNMSTDFIPMSVFSESSQ    KFTVEGKILNKFDMKPHDQN
Sbjct: 85  FTMELAGTDSGNTLRSYSLNMSTDFIPMSVFSESSQ---GKFTVEGKILNKFDMKPHDQN 144

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           LERYGKLCRERTHKSMTKSRQ                                 IQVIDH
Sbjct: 145 LERYGKLCRERTHKSMTKSRQ---------------------------------IQVIDH 204

Query: 121 VTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQ 180
           VTGGHMRPMPGMDVLSFGAAEKKKVV+KGSETKRLRKERGELEKIIFRLFERQ+YWTSKQ
Sbjct: 205 VTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQSYWTSKQ 264

Query: 181 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETK 227
           LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKES EE K
Sbjct: 265 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESGEEAK 274

[Close]

BLAST of Lsi02G017100 vs. NCBI nr
Match: XP_008465665.1 (PREDICTED: general transcription factor IIF subunit 2 isoform X2 [Cucumis melo])

HSP 1 Score: 345.5 bits (885), Expect = 3.5e-91
Identity = 184/228 (80.70%), Postives = 187/228 (82.02%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           FTMELA TDSGNALRSY+LNMSTDFIPMSVFSESSQ    KFTVEGKILNKFDMKPHDQN
Sbjct: 86  FTMELASTDSGNALRSYTLNMSTDFIPMSVFSESSQ---GKFTVEGKILNKFDMKPHDQN 145

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           LERYGKLCRERTHKSMTKSRQ                                 IQVIDH
Sbjct: 146 LERYGKLCRERTHKSMTKSRQ---------------------------------IQVIDH 205

Query: 121 VTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQ 180
           VTGGHMRPMPGMDVLSFGAAEKKKVV+KGSETKRLRKERGELEKIIF+LFERQ YWTSKQ
Sbjct: 206 VTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQ 265

Query: 181 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR 229
           LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKES EETK R
Sbjct: 266 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESSEETKPR 277

[Close]

BLAST of Lsi02G017100 vs. NCBI nr
Match: XP_031741396.1 (general transcription factor IIF subunit 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 344.0 bits (881), Expect = 1.0e-90
Identity = 182/228 (79.82%), Postives = 187/228 (82.02%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           FTMELA TDSGNALRSYSLNMSTDFIPMSVFSESSQ    KFT+EGKILNKFDMKPHDQN
Sbjct: 149 FTMELASTDSGNALRSYSLNMSTDFIPMSVFSESSQ---GKFTIEGKILNKFDMKPHDQN 208

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           LERYGKLCRERTHKSMTKSRQ                                 IQVIDH
Sbjct: 209 LERYGKLCRERTHKSMTKSRQ---------------------------------IQVIDH 268

Query: 121 VTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQ 180
           VTGGHMRPMPGMDVLSFGAAEKKKVV+KGSETKRLRKERGELEKIIF+LFERQ YWTSKQ
Sbjct: 269 VTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQ 328

Query: 181 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR 229
           LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEY+ES E+TK R
Sbjct: 329 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYRESSEDTKPR 340

[Close]

BLAST of Lsi02G017100 vs. NCBI nr
Match: XP_004143805.1 (general transcription factor IIF subunit 2 isoform X3 [Cucumis sativus] >KGN51637.2 hypothetical protein Csa_008814 [Cucumis sativus])

HSP 1 Score: 344.0 bits (881), Expect = 1.0e-90
Identity = 182/228 (79.82%), Postives = 187/228 (82.02%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           FTMELA TDSGNALRSYSLNMSTDFIPMSVFSESSQ    KFT+EGKILNKFDMKPHDQN
Sbjct: 86  FTMELASTDSGNALRSYSLNMSTDFIPMSVFSESSQ---GKFTIEGKILNKFDMKPHDQN 145

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           LERYGKLCRERTHKSMTKSRQ                                 IQVIDH
Sbjct: 146 LERYGKLCRERTHKSMTKSRQ---------------------------------IQVIDH 205

Query: 121 VTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQ 180
           VTGGHMRPMPGMDVLSFGAAEKKKVV+KGSETKRLRKERGELEKIIF+LFERQ YWTSKQ
Sbjct: 206 VTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQ 265

Query: 181 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEETKLR 229
           LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEY+ES E+TK R
Sbjct: 266 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYRESSEDTKPR 277

[Close]

BLAST of Lsi02G017100 vs. TAIR 10
Match: AT3G52270.1 (Transcription initiation factor IIF, beta subunit )

HSP 1 Score: 225.3 bits (573), Expect = 4.9e-59
Identity = 120/224 (53.57%), Postives = 153/224 (68.30%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           F MELA  DSGN  R Y+L+MS DFIPM+VF ESS     K +VEGKI NKFDM+PH++N
Sbjct: 83  FVMELARADSGNMPRRYTLDMSKDFIPMNVFCESSD---GKMSVEGKIKNKFDMRPHNEN 142

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           +E YG+LCRERT+K M K+RQ                                 IQVID+
Sbjct: 143 IESYGRLCRERTNKYMGKNRQ---------------------------------IQVIDN 202

Query: 121 VTGGHMRPMPGMDVLSFGAAEKKKVVTKGSETKRLRKERGELEKIIFRLFERQAYWTSKQ 180
             G HMRPMPGM ++   A EKKK+  + SE KR R++R E+E+++F LFERQ+ WT + 
Sbjct: 203 ARGMHMRPMPGM-IIPTAAPEKKKLTNRTSEMKRTRRDRREMEEVMFNLFERQSNWTLRL 262

Query: 181 LIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEE 225
           LIQETDQPEQ++K++LKDLC+YNNKG +QGTYELKPEYK++ +E
Sbjct: 263 LIQETDQPEQFLKDLLKDLCIYNNKGSNQGTYELKPEYKKATQE 269

[Close]

BLAST of Lsi02G017100 vs. TAIR 10
Match: AT1G75510.1 (Transcription initiation factor IIF, beta subunit )

HSP 1 Score: 192.6 bits (488), Expect = 3.5e-49
Identity = 108/226 (47.79%), Postives = 141/226 (62.39%), Query Frame = 0


Query: 1   FTMELAGTDSGNALRSYSLNMSTDFIPMSVFSESSQETLRKFTVEGKILNKFDMKPHDQN 60
           F M + G + GN  + Y+LNM TDF+PM  FS+ +Q        EGK+ +KFDMKP+ + 
Sbjct: 67  FKMYMVGAEYGNMPKCYALNMFTDFVPMGGFSDVNQGCA---AAEGKVDHKFDMKPYGET 126

Query: 61  LERYGKLCRERTHKSMTKSRQIQVSFVTSYAVYLGVWLLIYCSHDLLVKISKYSIQVIDH 120
           +E Y +LCRERT K+M K+RQ                                 IQVID+
Sbjct: 127 IEEYARLCRERTSKAMVKNRQ---------------------------------IQVIDN 186

Query: 121 VTGGHMRPMPGM-DVLSFGAAEKKK-VVTKGSETKRLRKERGELEKIIFRLFERQAYWTS 180
             G HMRPMPGM  ++S  + EK+K    K +E KR R++RGELE I+F+LFE Q  WT 
Sbjct: 187 DRGVHMRPMPGMLGLVSSNSKEKRKPPPVKQTEVKRTRRDRGELEAIMFKLFEGQPNWTL 246

Query: 181 KQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESCEE 225
           KQL+QETDQP Q++KEIL +LCVYN +G +QGTYELKPEYK+S E+
Sbjct: 247 KQLVQETDQPAQFLKEILNELCVYNKRGSNQGTYELKPEYKKSAED 256

The following BLAST results are available for this feature:

BLAST of Lsi02G017100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q03123	1.1e-10	41.77	General transcription factor IIF subunit 2 OS=Xenopus laevis OX=8355 GN=gtf2f2 P...	[more]
Q2T9L9	1.8e-10	27.50	General transcription factor IIF subunit 2 OS=Bos taurus OX=9913 GN=GTF2F2 PE=2 ...	[more]
P13984	1.8e-10	28.00	General transcription factor IIF subunit 2 OS=Homo sapiens OX=9606 GN=GTF2F2 PE=...	[more]
Q8R0A0	4.1e-10	27.00	General transcription factor IIF subunit 2 OS=Mus musculus OX=10090 GN=Gtf2f2 PE...	[more]
P41900	7.0e-10	40.51	General transcription factor IIF subunit 2 OS=Drosophila melanogaster OX=7227 GN...	[more]

BLAST of Lsi02G017100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CPE2	2.6e-92	80.09	Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A1S3CPB8	1.7e-91	80.70	Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1BZ89	3.2e-90	79.82	Transcription initiation factor IIF subunit beta OS=Momordica charantia OX=3673 ...	[more]
A0A6J1JIQ3	7.1e-90	78.95	Transcription initiation factor IIF subunit beta OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1HK34	7.1e-90	78.95	Transcription initiation factor IIF subunit beta OS=Cucurbita moschata OX=3662 G...	[more]

BLAST of Lsi02G017100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008465664.1	5.4e-92	80.09	PREDICTED: general transcription factor IIF subunit 2 isoform X1 [Cucumis melo]	[more]
XP_038889594.1	2.7e-91	81.42	general transcription factor IIF subunit 2-like [Benincasa hispida] >XP_03888959...	[more]
XP_008465665.1	3.5e-91	80.70	PREDICTED: general transcription factor IIF subunit 2 isoform X2 [Cucumis melo]	[more]
XP_031741396.1	1.0e-90	79.82	general transcription factor IIF subunit 2 isoform X2 [Cucumis sativus]	[more]
XP_004143805.1	1.0e-90	79.82	general transcription factor IIF subunit 2 isoform X3 [Cucumis sativus] >KGN5163...	[more]

BLAST of Lsi02G017100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52270.1	4.9e-59	53.57	Transcription initiation factor IIF, beta subunit	[more]
AT1G75510.1	3.5e-49	47.79	Transcription initiation factor IIF, beta subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 154..220 e-value: 2.6E-30 score: 106.3
IPR040450	TFIIF beta subunit, HTH domain	PFAM	PF02270	TFIIF_beta	coord: 156..219 e-value: 7.6E-22 score: 77.2
IPR003196	Transcription initiation factor IIF, beta subunit	PANTHER	PTHR10445	GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2	coord: 113..225 coord: 1..84
None	No IPR available	PANTHER	PTHR10445:SF2	TRANSCRIPTION INITIATION FACTOR IIF, BETA SUBUNIT	coord: 113..225
None	No IPR available	PANTHER	PTHR10445:SF2	TRANSCRIPTION INITIATION FACTOR IIF, BETA SUBUNIT	coord: 1..84
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 155..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G017100.1	Lsi02G017100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0006366	transcription by RNA polymerase II
cellular_component	GO:0005674	transcription factor TFIIF complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008168	methyltransferase activity