Lsi02G016040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G016040
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptiontRNA pseudouridine synthase B
Locationchr02: 21912251 .. 21916648 (+)
RNA-Seq ExpressionLsi02G016040
SyntenyLsi02G016040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAACATTCACACACCGCCCAGCCCACCAAAAATGTTGAGAGTCTCACGAACTCCCTCAGCCATCTAACACTGCTTCACTTCAATGGCGAAATCATCTGCTCTGTTTTACGCCTCCCATTTCTTCATCCGCTCTACACTCATGGCTTCTTCCAAATCACTCACTCTCTTCCTTCCACTCTCAAACCTCAACCATCTCCACAGGTTTTCCTTTCGCAATTCACTCTCTTCCTTATCGCTCCGCAGATTCTCCACCACTTCAACCCCATTCCCTCTGCAATACGAAATGATCATCAACCGACCGTCTTATCCTCCCCCGCCCCATCAGAATCGACGAACGCCCGCCAGAATCAGCTCCGACAATTCTTCCGAGCTCGACTCCTCCGAGGATCCCACTTCCGAATTGGGGTTCGAGAGCTGGGTGGATCGGAAGCTTATATCCGACAGTGAAACTGTGTCCGGTAAGGAAGGCGTGGTCATGGATAAGGCTATGAGGAAATACTATAACAAAAGAAGGAAGAGGATGTATGGGTCTGATTCTGATGAAGATAATAAGACACAGGACGAGGGATTCGTGGAATTAAAGCCTGAGGTGGTTGAGTTTAACACATTGCATAAGAGAGAGGAGGAATTGTTCTTCTATGACGCGTTTGCGTATCCATGGGAGAAAGATAAGCATTACAAAATGGTGTATCAGTTGGAGAATAAATATTTTCCTGATGAAGGTCTTGACAAGGCTTTTCTGGGACCTGGAGAGTCAAATGTGGAAGTGAATGAACAGACAAAGGGAAGGAAGGGGGTGAAAAAGGCAGTGAGGGTTAAGCCCGAGACGAAAGTGGAGGTCCCCAATGGCATGAATGACAAAGGGTTGGTTTTCTTTGATGAGGGGAGACCAGAAAAGGAAAATAAAGGGTCCATGAAAGATGTGGCAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAAGTTCCTGGTAAAGATTCTGAAATTGGTCAGACGGAGCCATATCTTTTGACGAGGCATACGGAGCTCCCTGCCAAATGGGATAGTCCTTGTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGTCAGCCTGGGGTTTCTGCAGTCCTTTGAAAGGAAATACCCTGTTTATAATCATTGATTTGATATCTTGGTGAAAGTTCTGTATCCATTCACATGTTCGTTCTCTAATTTTGCATTGTTTCATTAGAAGTAAATTCCTCACCCTTTTTCTTTTTGACGTTTAAAGAAAACAACATGATTGTTTAACGTAGGGTGGACTTCATTTACGGTATGTGGAAAACTTCGGCGCCTAGTCAAAGTTAAAAAGGTGCATTTCAGCTGTTGCATCTGGCAGACTAAATTATTCGATTGTCTGAGCTCCTCTAACTACATTGTTTTCCACTTATGTGCATTGATAGGTAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGCGTTGGAAAAGCGACAAAGTTGGTAGACAGGTGATGACTAAGTTAGCCATGTAGTTGCTATTTTGTTTTTCATTTCCCCCCTACCCTTTTCCAAGTTAGATATTCTTTTGTTAATAGAATAAAATAATAAGAACAAGAAATAAAGGGTAAGAGACGACATGTGTTCCCAAGCCAAAGTCAAAATAGATTTCGAAATCACATGTACTAGCATTGGTGTAATGGGAAGAGTGATTACAGAAAACCTGAGGCATACAACTTTTCTAAGAGGTGGCATGTTTATCTTTTAGATATCAAGGTATGATTAAGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTGTGTGATCTTATCTCTCTAGTTAATTATGGTTTCTTATTTTTAACTGATATGCATCTATGTACTCTATCTGCTGTGTTCTTTTGGGAAGTATGATGTATCTCTTTACTATATCTACTGTTAATGGATCTGTCAGAGTAATAATTATTCTAGTCTTAAGGAAGAAGATATATTATTCTATATTAACTCATCAAATATTTGCCCTGGCTAGTCCGTCACAATAGACATGAAGAGATTTTGGAATTATATTGTATATAAAGGGAGGCTCCATTTTATATGTGTCCTATCCTTTCTCCATCATTAGCATAATGTTTGGCAACATTCAGTTAGTTGTTGAGTTGTTAGGTTAATTGCAAGGTGAGGTTTTGACTTGTCTCACAGGTAAATAAAAAAATTCAATTGGGTTCCGTTCCTGATGAAAATTTATTTGTATATAATCCAAATAAGAAACACTTGTCCCAATTGTTATGCAGGTAATTCAGCGAGAGCCTTGGGAGCACATCAAAGATGATGACATCAAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTTCCTCCCATGTTTTCTGCCATTAAAGTAAGCACTTCGTCTTTCTATAATTATATATTGCATAATAGAAGCAATGTTGTTAAAAATGCATGTTACACAGAATCACAAAGCAAACTCATGATTCATATCATATCTGCATGGAATTTTTACAACTCGGATGAGGGGAACAAAAATTTGTTTAAAAATAAGCTTTTCTTTCAATATACTTTGTGGTATAAGATTATGGTTAGTACAGAGGTCAATTTAATGGATGGTACACTAGTACAGTAAGTTAAGGAACATTTGAAAGCCTTTATTTATTTATGTTATTATTATTATTATTTTGTAAACTTCATTTCATCTGTTCTTTAAGGATGTGTTTCTTGTCTGTTCCACATTTCCTCATCTGTATAGCATCTCTTTTGTGAAAGATGCTTATATTGATGTTCTTTTATCACTAGGGCTTTCTCTCTTATACATGTCATCCATAGAAAGTTTGTTTTTGTGTCAAAAAATATGATGGTTCGGTTCTCTGATTGTGTTATGCTTTTGTGGAGCCGGCCAACTAGAATGACAATATTTCATGCTTCTCCATTCAGAAATTATGAGTACTTGTCAAGATGAAATTGATGTTCGGGGAGATCTTGGCTGCATGAGACTGATTTGATTACAAATTTCATTACCTTGTTTTTAGGTCGGAGGTGAGAAGATGTATGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTTTCTCCGAGACGGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAGATGACAGGTAGGTTCACTTCCAACGTACACTTCTGTTTCATCATTTCTATAATTTGTGGCTAGGCCATATGCAAATGCATCGAGCACTTGAAGATGTCCAATTGATTGTTAACAGAAAGAAATTTGTCTAGCTAAAATTGTGGCATTTCACTATATAAGATAGCGCTAGATCATTAGGTGGCATGGGTTTAGAAACTTCTGTTTTGATGCGAATCCAACTTTCTTATACAAAGACGAAAGTAGCAAAAAAGAGGCCATATAGAAACACAGGCCAAAATAAGGAGTCCTGAAAGAGTTACAGGAGAAGACTTCAATTTCAGTGATTAAACATGATGGAAAATCACAAAAGGTCCAAATTTTCAGATATTTTACGGCTGACTCACTATATTGGATATTTGATAAGGCCAATAACTTGACTCATTTGTTTACTTGATGAGCTCTAGCTGGTTTGTCGGACAGCTCAAATATACTTGTTAGTTTTTTCAAAATTTCTGTTGAAAATGTTGTTTTGTTGCACTAATATGTGTCAACTTCTTAATTTAGTGAATATGTTCTCTTTCAAAGATTTTAATGTGTTCTGGCTGACTGGACTGTGACAGGCAAAATTTGATTTTTCGAGTTACATGCTCTAAAGGAACATACATTCGGTCACTTTGTGCAGATCTTGGGAAGGTTCTTGGCAGGTATTCATCCTCCCCCTTGCAATGGATCTTCATTTCTGTACATTCTTTTACCTTTATTACCGAGAAATGCTTCAAAGAGTAAATCCGTGTCAAGCTTCTGGTTCGTTTTGATCCTCTCTAGTTTAATGTTGAAATGTACTATTCTTTGTCTATTGCAGTTGTGCCCATCTAACTGCTCTTCGAAGGGATTCAATAGGTCAGTTCAAGAGTCGTTATTTATTTTTTATTTAATCATATTGGCTCAACGCTCAAATGTTATCGAAAAGTCTCATCAAGATTGTAAAGGACACTAACTACTTGGTTATATTATTCCATATCAAGTTCAAAAGAAAAGAAAAAAACTTTAAAGTTCAGTTCATCATATCAAAGTATCACCTTTTCATTATTTTACAAAGTATTGGCAGTGGTAGTAGCTGAACTAAATATCCAAGACAATGTGATTTTGACATGAGCCTCCCCATTTCAGGGCAATATCTAGCAGATGATGCCTGGGAATTCAAAGAGTTGGAAGATGCAATTACCAAAGGTTATTTTTAG

mRNA sequence

CAAACATTCACACACCGCCCAGCCCACCAAAAATGTTGAGAGTCTCACGAACTCCCTCAGCCATCTAACACTGCTTCACTTCAATGGCGAAATCATCTGCTCTGTTTTACGCCTCCCATTTCTTCATCCGCTCTACACTCATGGCTTCTTCCAAATCACTCACTCTCTTCCTTCCACTCTCAAACCTCAACCATCTCCACAGGTTTTCCTTTCGCAATTCACTCTCTTCCTTATCGCTCCGCAGATTCTCCACCACTTCAACCCCATTCCCTCTGCAATACGAAATGATCATCAACCGACCGTCTTATCCTCCCCCGCCCCATCAGAATCGACGAACGCCCGCCAGAATCAGCTCCGACAATTCTTCCGAGCTCGACTCCTCCGAGGATCCCACTTCCGAATTGGGGTTCGAGAGCTGGGTGGATCGGAAGCTTATATCCGACAGTGAAACTGTGTCCGGTAAGGAAGGCGTGGTCATGGATAAGGCTATGAGGAAATACTATAACAAAAGAAGGAAGAGGATGTATGGGTCTGATTCTGATGAAGATAATAAGACACAGGACGAGGGATTCGTGGAATTAAAGCCTGAGGTGGTTGAGTTTAACACATTGCATAAGAGAGAGGAGGAATTGTTCTTCTATGACGCGTTTGCGTATCCATGGGAGAAAGATAAGCATTACAAAATGGTGTATCAGTTGGAGAATAAATATTTTCCTGATGAAGGTCTTGACAAGGCTTTTCTGGGACCTGGAGAGTCAAATGTGGAAGTGAATGAACAGACAAAGGGAAGGAAGGGGGTGAAAAAGGCAGTGAGGGTTAAGCCCGAGACGAAAGTGGAGGTCCCCAATGGCATGAATGACAAAGGGTTGGTTTTCTTTGATGAGGGGAGACCAGAAAAGGAAAATAAAGGGTCCATGAAAGATGTGGCAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAAGTTCCTGGTAAAGATTCTGAAATTGGTCAGACGGAGCCATATCTTTTGACGAGGCATACGGAGCTCCCTGCCAAATGGGATAGTCCTTGTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGCGTTGGAAAAGCGACAAAGTTGGTAGACAGATATCAAGGTATGATTAAGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCACATCAAAGATGATGACATCAAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTTCCTCCCATGTTTTCTGCCATTAAAGTCGGAGGTGAGAAGATGTATGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTTTCTCCGAGACGGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAGATGACAGGCAAAATTTGATTTTTCGAGTTACATGCTCTAAAGGAACATACATTCGGTCACTTTGTGCAGATCTTGGGAAGGTTCTTGGCAGTTGTGCCCATCTAACTGCTCTTCGAAGGGATTCAATAGGGCAATATCTAGCAGATGATGCCTGGGAATTCAAAGAGTTGGAAGATGCAATTACCAAAGGTTATTTTTAG

Coding sequence (CDS)

ATGGCGAAATCATCTGCTCTGTTTTACGCCTCCCATTTCTTCATCCGCTCTACACTCATGGCTTCTTCCAAATCACTCACTCTCTTCCTTCCACTCTCAAACCTCAACCATCTCCACAGGTTTTCCTTTCGCAATTCACTCTCTTCCTTATCGCTCCGCAGATTCTCCACCACTTCAACCCCATTCCCTCTGCAATACGAAATGATCATCAACCGACCGTCTTATCCTCCCCCGCCCCATCAGAATCGACGAACGCCCGCCAGAATCAGCTCCGACAATTCTTCCGAGCTCGACTCCTCCGAGGATCCCACTTCCGAATTGGGGTTCGAGAGCTGGGTGGATCGGAAGCTTATATCCGACAGTGAAACTGTGTCCGGTAAGGAAGGCGTGGTCATGGATAAGGCTATGAGGAAATACTATAACAAAAGAAGGAAGAGGATGTATGGGTCTGATTCTGATGAAGATAATAAGACACAGGACGAGGGATTCGTGGAATTAAAGCCTGAGGTGGTTGAGTTTAACACATTGCATAAGAGAGAGGAGGAATTGTTCTTCTATGACGCGTTTGCGTATCCATGGGAGAAAGATAAGCATTACAAAATGGTGTATCAGTTGGAGAATAAATATTTTCCTGATGAAGGTCTTGACAAGGCTTTTCTGGGACCTGGAGAGTCAAATGTGGAAGTGAATGAACAGACAAAGGGAAGGAAGGGGGTGAAAAAGGCAGTGAGGGTTAAGCCCGAGACGAAAGTGGAGGTCCCCAATGGCATGAATGACAAAGGGTTGGTTTTCTTTGATGAGGGGAGACCAGAAAAGGAAAATAAAGGGTCCATGAAAGATGTGGCAGAGAAGAAGGTGGAGGAGTTCTTCAAGTGTTTGAAGAAAGTTCCTGGTAAAGATTCTGAAATTGGTCAGACGGAGCCATATCTTTTGACGAGGCATACGGAGCTCCCTGCCAAATGGGATAGTCCTTGTGGGACAGTGGTTTTGCTGAACAAACCTAAAGGTCATGCAGGTACTCTTGATCCTATGGCTACTGGTTTGTTGATAGTGTGCGTTGGAAAAGCGACAAAGTTGGTAGACAGATATCAAGGTATGATTAAGAGCTACAGTGGAGTTTTCCGTTTAGGAGAGGCTACTTCAACTTGGGATGCTGATTCACCGGTAATTCAGCGAGAGCCTTGGGAGCACATCAAAGATGATGACATCAAAAAAGCTGCTGCCTCTTTTTGTGGGGAAATTTGGCAAGTTCCTCCCATGTTTTCTGCCATTAAAGTCGGAGGTGAGAAGATGTATGAGAAAGCAAGAAGGGGAGAAAGTATTGAGCTTTCTCCGAGACGGATATCAATTTTCAAATTTGATATTGAACGTAGTTTAGATGACAGGCAAAATTTGATTTTTCGAGTTACATGCTCTAAAGGAACATACATTCGGTCACTTTGTGCAGATCTTGGGAAGGTTCTTGGCAGTTGTGCCCATCTAACTGCTCTTCGAAGGGATTCAATAGGGCAATATCTAGCAGATGATGCCTGGGAATTCAAAGAGTTGGAAGATGCAATTACCAAAGGTTATTTTTAG

Protein sequence

MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTSTPFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISDSETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKREEELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVKKAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKDSEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPKGHAGTLDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLDDRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAITKGYF
Homology
[Close]
BLAST of Lsi02G016040 vs. ExPASy Swiss-Prot
Match: Q9RB36 (tRNA pseudouridine synthase B OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=truB PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 4.9e-43
Identity = 97/214 (45.33%), Postives = 128/214 (59.81%), Query Frame = 0 

Query: 318 PAKWDSPCGT----VVLLNK---PK----GHAGTLDPMATGLLIVCVGKATKLVDRYQGM 377
           P KW S         +L+NK   PK    GHAGTLDP+ATGLL++C GK TK +   QG 
Sbjct: 17  PLKWSSFQAVNKLKYLLINKVGLPKKFKIGHAGTLDPLATGLLLICTGKFTKKISELQGQ 76

Query: 378 IKSYSGVFRLGEATSTWDADSPVIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIKV 437
            K Y+G F +G  T ++D ++ + Q  P EHI +  I +    F GEI Q PP+FSAIK 
Sbjct: 77  AKEYTGTFYIGATTPSYDLETEIDQTFPTEHINEVLIHETVKQFLGEIDQKPPIFSAIKK 136

Query: 438 GGEKMYEKARRGESIELSPRRISIFKFDIERSLDDRQNLIFRVTCSKGTYIRSLCADLGK 497
            G ++YE AR GESIE+  R+ +I +F+I R       + FRV CSKGTYIRSL  D GK
Sbjct: 137 DGVRLYEHARAGESIEIESRKTTIHEFEITRIA--LPEIDFRVVCSKGTYIRSLAYDFGK 196

Query: 498 VLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 521
            + S +HLT LRR  IG Y   +A +    E+++
Sbjct: 197 AMNSGSHLTVLRRTKIGDYDVKNAIDITLFEESL 228

[Close]
BLAST of Lsi02G016040 vs. ExPASy Swiss-Prot
Match: A6LEL7 (tRNA pseudouridine synthase B OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152) OX=435591 GN=truB PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.6e-38
Identity = 80/183 (43.72%), Postives = 114/183 (62.30%), Query Frame = 0 

Query: 336 GHAGTLDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPW 395
           GHAGTLDP+ATG++IVC G+ATK +D +Q   K Y    +LGE T ++D +  +    P 
Sbjct: 42  GHAGTLDPLATGVMIVCTGRATKRIDEFQYQTKEYIATLKLGETTPSFDLEKEIDAVYPT 101

Query: 396 EHIKDDDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDI 455
           EHI  + +++   +F G I Q+PP+FSA KV G++ YE AR+GE +EL  + + I + ++
Sbjct: 102 EHITRELVEEVLKTFVGTIQQIPPVFSACKVEGKRAYELARKGEEVELKSKTLVIDELEL 161

Query: 456 ERSLDDRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKE 515
                D   +  RV CSKGTYIR+L  D+GK LGS AHL  L R  IG+   D     +E
Sbjct: 162 LEC--DLPVIKIRVVCSKGTYIRALARDIGKALGSGAHLIGLERTRIGEVTLDMCMSPEE 221

Query: 516 LED 519
           ++D
Sbjct: 222 IDD 222

[Close]
BLAST of Lsi02G016040 vs. ExPASy Swiss-Prot
Match: A0JUU8 (tRNA pseudouridine synthase B OS=Arthrobacter sp. (strain FB24) OX=290399 GN=truB PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.8e-38
Identity = 81/189 (42.86%), Postives = 115/189 (60.85%), Query Frame = 0 

Query: 336 GHAGTLDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPW 395
           GHAGTLDPMATG+L++ + KAT+L+    G  K+Y+   RLGE+T T DA+  V+     
Sbjct: 33  GHAGTLDPMATGVLVLGINKATRLLTYIVGTSKTYTATIRLGESTVTDDAEGEVVSSHSA 92

Query: 396 EHIKDDDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDI 455
             + +  I+ A A+  GEI QVP   SAIKV GE+ Y + R GE ++L+ R ++I +FD+
Sbjct: 93  AAVTEGAIRAAVAALTGEIQQVPSSVSAIKVNGERAYARVRSGEDVKLAARPVTIHRFDV 152

Query: 456 ERSLDDRQNLIF----RVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAW 515
                +R   +      V CS GTYIR+L  DLG+ LG+  HLTALRR  +G Y  D A 
Sbjct: 153 HAVRPERAGAVLDVDVTVECSSGTYIRALARDLGEALGTGGHLTALRRTQVGPYTLDQAR 212

Query: 516 EFKELEDAI 521
             ++L + +
Sbjct: 213 TLEQLAEEL 221

[Close]
BLAST of Lsi02G016040 vs. ExPASy Swiss-Prot
Match: A3PY88 (tRNA pseudouridine synthase B OS=Mycobacterium sp. (strain JLS) OX=164757 GN=truB PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.8e-38
Identity = 84/185 (45.41%), Postives = 117/185 (63.24%), Query Frame = 0 

Query: 336 GHAGTLDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPW 395
           GHAGTLDPMATG+L+V + +ATKL+       KSY    RLG+ T+T DA+  V++  P 
Sbjct: 38  GHAGTLDPMATGVLVVGIDRATKLLGLLTATDKSYEATIRLGQTTTTEDAEGDVVETTPA 97

Query: 396 EHIKDDDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDI 455
             I D+ I  A A+  GEI QVP   SAIKVGG++ Y+ AR G+++EL+ RR+ I +F++
Sbjct: 98  TGITDEQIGHAVAALRGEIDQVPSAVSAIKVGGQRAYKLAREGQTVELAARRVRIDRFEV 157

Query: 456 --ERSLDDRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEF 515
              R +D   ++   V CS GTYIR+L  D+G  LG   HLT LRR  +G++  D+A+  
Sbjct: 158 LAIRRVDGFVDVDVAVDCSSGTYIRALARDVGVTLGVGGHLTLLRRTRVGRFGLDEAYPL 217

Query: 516 KELED 519
             L D
Sbjct: 218 DALAD 222

[Close]
BLAST of Lsi02G016040 vs. ExPASy Swiss-Prot
Match: A1UET1 (tRNA pseudouridine synthase B OS=Mycobacterium sp. (strain KMS) OX=189918 GN=truB PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.8e-38
Identity = 84/185 (45.41%), Postives = 117/185 (63.24%), Query Frame = 0 

Query: 336 GHAGTLDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPW 395
           GHAGTLDPMATG+L+V + +ATKL+       KSY    RLG+ T+T DA+  V++  P 
Sbjct: 38  GHAGTLDPMATGVLVVGIDRATKLLGLLTATDKSYEATIRLGQTTTTEDAEGDVVETTPA 97

Query: 396 EHIKDDDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDI 455
             I D+ I  A A+  GEI QVP   SAIKVGG++ Y+ AR G+++EL+ RR+ I +F++
Sbjct: 98  TGITDEQIGHAVAALRGEIDQVPSAVSAIKVGGQRAYKLAREGQTVELAARRVRIDRFEV 157

Query: 456 --ERSLDDRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEF 515
              R +D   ++   V CS GTYIR+L  D+G  LG   HLT LRR  +G++  D+A+  
Sbjct: 158 LAIRRVDGFVDVDVAVDCSSGTYIRALARDVGVTLGVGGHLTLLRRTRVGRFGLDEAYPL 217

Query: 516 KELED 519
             L D
Sbjct: 218 DALAD 222

[Close]
BLAST of Lsi02G016040 vs. ExPASy TrEMBL
Match: A0A1S3C8B0 (uncharacterized protein LOC103498118 OS=Cucumis melo OX=3656 GN=LOC103498118 PE=3 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 6.3e-275
Identity = 483/545 (88.62%), Postives = 502/545 (92.11%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           MAKS ALF+ASHFFIRSTLM SSKSL LFLPLSNLNH HRF+FRNSLS LSLRRF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
           PFPLQYE+IINRPSYP PPHQN RTPARISSDN  EL+SSEDP+SELGF+SWVDRKLIS+
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
           S TVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+TQDEGFVELKPEVVEFNTLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLE KYFPD+GLDKAFLGPGESNVEVNEQTKGR+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           KAVRVKPET VEV NGM+DKG+VFFDEG+PEKENKGS+ DV EKKVEEFFKCLKK P KD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           S+IGQ EPYLLTRH ELPA+WDSPCGTVVLLNKPK                    GHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLVDRYQGMIK YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 526
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIGQ+LADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQHLADDAWEFKELEDAI 540

[Close]
BLAST of Lsi02G016040 vs. ExPASy TrEMBL
Match: A0A0A0KP03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G522940 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 1.9e-271
Identity = 478/545 (87.71%), Postives = 500/545 (91.74%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           MAKS ALF+ASHFFIRSTLM SSKSL+LFLPLSNLNHLHRF+FRNSLS LSLR FSTT T
Sbjct: 16  MAKSPALFHASHFFIRSTLMPSSKSLSLFLPLSNLNHLHRFAFRNSLSPLSLRTFSTT-T 75

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
            FPLQYE+IINRPSYP PPHQN RTPAR+SSDNS EL+SSEDPTSELGF+SWVDRKLIS+
Sbjct: 76  LFPLQYELIINRPSYPSPPHQNPRTPARVSSDNSPELNSSEDPTSELGFDSWVDRKLISE 135

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
             TVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+TQ EGFVELKPEVVEFNTLHKRE
Sbjct: 136 GGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQAEGFVELKPEVVEFNTLHKRE 195

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLE KYFPD+GLDKAFLGPGESNVEVNEQTKGR+GV+
Sbjct: 196 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVR 255

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           KA RVKPE  VEV NGM+DK +VFFDEG+PEKENKGS+ DV+EKKVEEFFKCLKK P KD
Sbjct: 256 KAGRVKPEMNVEVANGMDDKRMVFFDEGKPEKENKGSVVDVSEKKVEEFFKCLKKGPAKD 315

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           S IGQ EPYLLTRH ELPAKWDSPCGTVVLLNKPK                    GHAGT
Sbjct: 316 SNIGQGEPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 375

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 376 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 435

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDI+KAAASFCGEIWQVPPMFSAIKVGGE+MYEKARRGESIELSPR+ISIFKFDIERSLD
Sbjct: 436 DDIQKAAASFCGEIWQVPPMFSAIKVGGERMYEKARRGESIELSPRQISIFKFDIERSLD 495

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 526
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIGQYLADDAWEFKELEDAI
Sbjct: 496 DRQNLIFRVTCSKGTYIRSLCADLGKTLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 555

[Close]
BLAST of Lsi02G016040 vs. ExPASy TrEMBL
Match: A0A6J1JSH8 (uncharacterized protein LOC111489372 OS=Cucurbita maxima OX=3661 GN=LOC111489372 PE=3 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 2.1e-270
Identity = 478/545 (87.71%), Postives = 494/545 (90.64%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           MA S AL +ASHFFIRSTLMASSKSL  F PLSNLNHLHRF   NSLS LSLRR STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLHRFP--NSLSLLSLRRSSTTST 60

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
           PFPLQY+MIIN P YPP P QNRRTP RISSDNS EL+SSEDP SELGFESWVD+KLIS+
Sbjct: 61  PFPLQYDMIINHPIYPPMPPQNRRTPPRISSDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
           SE+ SGKEGVVMDKA RKYYNKRR+RMYGSDSDED KTQDEGFVELKPEVVEFNTLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRRRMYGSDSDEDKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFFYD FAYPWEKDKHYKMVYQLE KYFPDEGLDKAFLGPGESNVEVNEQTKGRKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           KAV VKPETKVEV NG+++KGLVFFD GRPEK+NKGS+KDV EKKVEEFFKCLKKVP KD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           SEIGQTEPYLLTRHTELPAKWDSPCGTVVL+NKPK                    GHAGT
Sbjct: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKL DRYQGMIKSYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISI+KFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 526
           DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIG+YLADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGEYLADDAWEFKELEDAI 540

[Close]
BLAST of Lsi02G016040 vs. ExPASy TrEMBL
Match: A0A6J1FDZ7 (uncharacterized protein LOC111444875 OS=Cucurbita moschata OX=3662 GN=LOC111444875 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.4e-268
Identity = 474/545 (86.97%), Postives = 491/545 (90.09%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           MA S AL +ASHFFIRSTLMASSKSL  F PLSNLNHLHRF   NSLS LSLRR STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLHRFP--NSLSLLSLRRSSTTST 60

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
           PFPLQY+MII+RP YPP P QNRRTPARIS DNS EL+SSEDP SELGFESWVD+KLIS+
Sbjct: 61  PFPLQYDMIIDRPIYPPMPPQNRRTPARISPDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
           SE+ SGKEGVVMDKA RKYYNKRRKRMYGSDSDE+ KTQDEGFVELKPEVVEFNTLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRKRMYGSDSDEEKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFFYD FAYPWEKDKHYKMVYQLE KYFPDEGLDKAFLGPGESNVEVNEQTKGRKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           KAV VKPETKVEV NG+++KGLVFFD GRPEK+NKGS+KDV EKKVEEFFKCLKKVP KD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           SEIGQ  PYLLTRHTELPAKWDSPCGTVVL+NKPK                    GHAGT
Sbjct: 301 SEIGQMGPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKL DRYQGMIKSYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKA ASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISI+KFDIERSLD
Sbjct: 421 DDIKKAVASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 526
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIG+YLADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGEYLADDAWEFKELEDAI 540

[Close]
BLAST of Lsi02G016040 vs. ExPASy TrEMBL
Match: A0A5A7T7S0 (TruB_N domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00150 PE=3 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.1e-262
Identity = 463/525 (88.19%), Postives = 482/525 (91.81%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           MAKS ALF+ASHFFIRSTLM SSKSL LFLPLSNLNH HRF+FRNSLS LSLRRF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
           PFPLQYE+IINRPSYP PPHQN RTPARISSDN  EL+SSEDP+SELGF+SWVDRKLIS+
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
           S TVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+TQDEGFVELKPEVVEFNTLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLE KYFPD+GLDKAFLGPGESNVEVNEQTKGR+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           KAVRVKPET VEV NGM+DKG+VFFDEG+PEKENKGS+ DV EKKVEEFFKCLKK P KD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           S+IGQ EPYLLTRH ELPA+WDSPCGTVVLLNKPK                    GHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLVDRYQGMIK YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQY 506
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIGQ+
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQF 524

[Close]
BLAST of Lsi02G016040 vs. NCBI nr
Match: XP_038891059.1 (uncharacterized protein LOC120080471 [Benincasa hispida])

HSP 1 Score: 966.8 bits (2498), Expect = 7.3e-278
Identity = 488/545 (89.54%), Postives = 502/545 (92.11%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           M KSSALF+AS+FFIRST M SS +LTLFL  SNLNHLHRF+FRNSLS LS RR STTST
Sbjct: 1   MVKSSALFHASNFFIRSTFMPSSITLTLFLLPSNLNHLHRFAFRNSLSPLSPRRLSTTST 60

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
           PFPLQYEMIINRPSYPPPPHQNRRTP RI SDNS E DSSEDPTSE+GFESWVDRKLIS+
Sbjct: 61  PFPLQYEMIINRPSYPPPPHQNRRTPTRIRSDNSPEFDSSEDPTSEMGFESWVDRKLISE 120

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
           SET+SGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE
Sbjct: 121 SETISGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFFYDAFAYPWEKDKHYKMVYQLE KYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK
Sbjct: 181 EELFFYDAFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           K V V+PE KV+V +GM+DKGLVFFDEG+PE ENKGS+KDV EKKVEEFFKCLKKVP KD
Sbjct: 241 KGVGVEPEMKVKVTHGMDDKGLVFFDEGKPENENKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           SEIG TEPYLLTRHTELPAKWDSPCGTVVLLNKPK                    GHAGT
Sbjct: 301 SEIGPTEPYLLTRHTELPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLV+RYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVERYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
            DIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD
Sbjct: 421 GDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 526
           DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 540

[Close]
BLAST of Lsi02G016040 vs. NCBI nr
Match: XP_008458830.1 (PREDICTED: uncharacterized protein LOC103498118 [Cucumis melo])

HSP 1 Score: 956.1 bits (2470), Expect = 1.3e-274
Identity = 483/545 (88.62%), Postives = 502/545 (92.11%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           MAKS ALF+ASHFFIRSTLM SSKSL LFLPLSNLNH HRF+FRNSLS LSLRRF  TST
Sbjct: 1   MAKSPALFHASHFFIRSTLMPSSKSLYLFLPLSNLNHPHRFAFRNSLSPLSLRRF-CTST 60

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
           PFPLQYE+IINRPSYP PPHQN RTPARISSDN  EL+SSEDP+SELGF+SWVDRKLIS+
Sbjct: 61  PFPLQYELIINRPSYPSPPHQNPRTPARISSDNPPELNSSEDPSSELGFDSWVDRKLISE 120

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
           S TVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+TQDEGFVELKPEVVEFNTLHKRE
Sbjct: 121 SGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLE KYFPD+GLDKAFLGPGESNVEVNEQTKGR+GVK
Sbjct: 181 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVK 240

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           KAVRVKPET VEV NGM+DKG+VFFDEG+PEKENKGS+ DV EKKVEEFFKCLKK P KD
Sbjct: 241 KAVRVKPETNVEVANGMDDKGMVFFDEGKPEKENKGSVTDVTEKKVEEFFKCLKKGPAKD 300

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           S+IGQ EPYLLTRH ELPA+WDSPCGTVVLLNKPK                    GHAGT
Sbjct: 301 SKIGQAEPYLLTRHMELPARWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVQKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLVDRYQGMIK YSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKGYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 526
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIGQ+LADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGQHLADDAWEFKELEDAI 540

[Close]
BLAST of Lsi02G016040 vs. NCBI nr
Match: XP_011655389.2 (uncharacterized protein LOC101215528 isoform X1 [Cucumis sativus] >KGN51348.1 hypothetical protein Csa_008523 [Cucumis sativus])

HSP 1 Score: 944.5 bits (2440), Expect = 3.9e-271
Identity = 478/545 (87.71%), Postives = 500/545 (91.74%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           MAKS ALF+ASHFFIRSTLM SSKSL+LFLPLSNLNHLHRF+FRNSLS LSLR FSTT T
Sbjct: 16  MAKSPALFHASHFFIRSTLMPSSKSLSLFLPLSNLNHLHRFAFRNSLSPLSLRTFSTT-T 75

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
            FPLQYE+IINRPSYP PPHQN RTPAR+SSDNS EL+SSEDPTSELGF+SWVDRKLIS+
Sbjct: 76  LFPLQYELIINRPSYPSPPHQNPRTPARVSSDNSPELNSSEDPTSELGFDSWVDRKLISE 135

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
             TVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDN+TQ EGFVELKPEVVEFNTLHKRE
Sbjct: 136 GGTVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNRTQAEGFVELKPEVVEFNTLHKRE 195

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFF+DAFAYPWEKDKHYKM+YQLE KYFPD+GLDKAFLGPGESNVEVNEQTKGR+GV+
Sbjct: 196 EELFFHDAFAYPWEKDKHYKMLYQLEKKYFPDDGLDKAFLGPGESNVEVNEQTKGRQGVR 255

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           KA RVKPE  VEV NGM+DK +VFFDEG+PEKENKGS+ DV+EKKVEEFFKCLKK P KD
Sbjct: 256 KAGRVKPEMNVEVANGMDDKRMVFFDEGKPEKENKGSVVDVSEKKVEEFFKCLKKGPAKD 315

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           S IGQ EPYLLTRH ELPAKWDSPCGTVVLLNKPK                    GHAGT
Sbjct: 316 SNIGQGEPYLLTRHMELPAKWDSPCGTVVLLNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 375

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD
Sbjct: 376 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 435

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDI+KAAASFCGEIWQVPPMFSAIKVGGE+MYEKARRGESIELSPR+ISIFKFDIERSLD
Sbjct: 436 DDIQKAAASFCGEIWQVPPMFSAIKVGGERMYEKARRGESIELSPRQISIFKFDIERSLD 495

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 526
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIGQYLADDAWEFKELEDAI
Sbjct: 496 DRQNLIFRVTCSKGTYIRSLCADLGKTLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 555

[Close]
BLAST of Lsi02G016040 vs. NCBI nr
Match: XP_022993331.1 (uncharacterized protein LOC111489372 [Cucurbita maxima] >XP_022993332.1 uncharacterized protein LOC111489372 [Cucurbita maxima])

HSP 1 Score: 941.0 bits (2431), Expect = 4.3e-270
Identity = 478/545 (87.71%), Postives = 494/545 (90.64%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           MA S AL +ASHFFIRSTLMASSKSL  F PLSNLNHLHRF   NSLS LSLRR STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLHRFP--NSLSLLSLRRSSTTST 60

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
           PFPLQY+MIIN P YPP P QNRRTP RISSDNS EL+SSEDP SELGFESWVD+KLIS+
Sbjct: 61  PFPLQYDMIINHPIYPPMPPQNRRTPPRISSDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
           SE+ SGKEGVVMDKA RKYYNKRR+RMYGSDSDED KTQDEGFVELKPEVVEFNTLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRRRMYGSDSDEDKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFFYD FAYPWEKDKHYKMVYQLE KYFPDEGLDKAFLGPGESNVEVNEQTKGRKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLEKKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           KAV VKPETKVEV NG+++KGLVFFD GRPEK+NKGS+KDV EKKVEEFFKCLKKVP KD
Sbjct: 241 KAVGVKPETKVEVTNGVDEKGLVFFDGGRPEKDNKGSVKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           SEIGQTEPYLLTRHTELPAKWDSPCGTVVL+NKPK                    GHAGT
Sbjct: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKL DRYQGMIKSYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISI+KFDIERSLD
Sbjct: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 526
           DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIG+YLADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGEYLADDAWEFKELEDAI 540

[Close]
BLAST of Lsi02G016040 vs. NCBI nr
Match: XP_023550635.1 (uncharacterized protein LOC111808717 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 935.6 bits (2417), Expect = 1.8e-268
Identity = 475/545 (87.16%), Postives = 490/545 (89.91%), Query Frame = 0 

Query: 1   MAKSSALFYASHFFIRSTLMASSKSLTLFLPLSNLNHLHRFSFRNSLSSLSLRRFSTTST 60
           MA S AL +ASHFFIRSTLMASSKSL  F PLSNLNHLHRF   NSLS LSLRR STTST
Sbjct: 1   MATSPALSHASHFFIRSTLMASSKSLAFFFPLSNLNHLHRFP--NSLSLLSLRRSSTTST 60

Query: 61  PFPLQYEMIINRPSYPPPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISD 120
           PFPLQY+MI+NRP YPP P QNRRTP RIS DNS EL+SSEDP SELGFESWVD+KLIS+
Sbjct: 61  PFPLQYDMIVNRPIYPPMPPQNRRTPPRISPDNSPELESSEDPVSELGFESWVDQKLISE 120

Query: 121 SETVSGKEGVVMDKAMRKYYNKRRKRMYGSDSDEDNKTQDEGFVELKPEVVEFNTLHKRE 180
           SE+ SGKEGVVMDKA RKYYNKRRKRMYGSDSDED KTQDEGFVELKPEVVEFNTLHKRE
Sbjct: 121 SESASGKEGVVMDKAKRKYYNKRRKRMYGSDSDEDKKTQDEGFVELKPEVVEFNTLHKRE 180

Query: 181 EELFFYDAFAYPWEKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVK 240
           EELFFYD FAYPWEKDKHYKMVYQLE KYFPDEGLDKAFLGPGESNVEVNEQTKGRKGV+
Sbjct: 181 EELFFYDTFAYPWEKDKHYKMVYQLERKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVR 240

Query: 241 KAVRVKPETKVEVPNGMNDKGLVFFDEGRPEKENKGSMKDVAEKKVEEFFKCLKKVPGKD 300
           KAV VKPETK+EV NG++ KGLVFFD GRPEK+NKGS KDV EKKVEEFFKCLKKVP KD
Sbjct: 241 KAVGVKPETKLEVTNGVDGKGLVFFDGGRPEKDNKGSAKDVTEKKVEEFFKCLKKVPAKD 300

Query: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGT 360
           SEIGQTEPYLLTRHTELPAKWDSPCGTVVL+NKPK                    GHAGT
Sbjct: 301 SEIGQTEPYLLTRHTELPAKWDSPCGTVVLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGT 360

Query: 361 LDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKD 420
           LDPMATGLLIVCVGKATKL DRYQGMIKSYSGVFRLGEATSTWDADS VIQREPWEHIKD
Sbjct: 361 LDPMATGLLIVCVGKATKLADRYQGMIKSYSGVFRLGEATSTWDADSAVIQREPWEHIKD 420

Query: 421 DDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLD 480
           DDIKKA ASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISI+KFDIERSLD
Sbjct: 421 DDIKKAVASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIYKFDIERSLD 480

Query: 481 DRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAI 526
           DRQNLIFRVTCSKGTYIRSLCADLGK LGSCAHLTALRRDSIG+YLADDAWEFKELEDAI
Sbjct: 481 DRQNLIFRVTCSKGTYIRSLCADLGKALGSCAHLTALRRDSIGEYLADDAWEFKELEDAI 540

[Close]
BLAST of Lsi02G016040 vs. TAIR 10
Match: AT5G14460.1 (Pseudouridine synthase family protein )

HSP 1 Score: 594.3 bits (1531), Expect = 9.2e-170
Identity = 343/544 (63.05%), Postives = 394/544 (72.43%), Query Frame = 0 

Query: 19  LMASSKSLTLFLPL--SNLNHLHRFSFRNSLSSLSLRRFSTTSTPFPLQYEMIINRPSYP 78
           LM+SS  +++  PL  S++    R   +  L+SL L   STTST +PLQY+MIINRP+  
Sbjct: 10  LMSSSARMSMTSPLFFSSVKP-WRNIHKPYLASLFL---STTSTRYPLQYDMIINRPT-Q 69

Query: 79  PPPHQNRRTPARISSDNSSELDSSEDPTSELGFESWVDRKLISDSET-VSGKEGVVMDKA 138
               QNRR P +        ++S    ++E  F+SWVD KL  + E    G     MDKA
Sbjct: 70  SSLSQNRRRPPK-------AIESGAPDSAEPEFDSWVDNKLAMEREQGRPGSGDPEMDKA 129

Query: 139 MRKYYNKRRKRMYGSDSDEDNKTQ--DEGFVELKPEVVEFNTLHKREEELFFYDAFAYPW 198
            RKYY+KRRKR+YGSDS+++N ++  DEGFVELKPEVVEF+ LH+REEEL+FYD FAYPW
Sbjct: 130 KRKYYSKRRKRLYGSDSEDENSSRKSDEGFVELKPEVVEFDRLHQREEELYFYDTFAYPW 189

Query: 199 EKDKHYKMVYQLENKYFPDEGLDKAFLGPGESNVEVNEQTKGRKGVKKAVRVKPETKVEV 258
           EKDKHYKMVYQLE KY+PD+ LDKAFL PGE   + ++  K R G KK V      + EV
Sbjct: 190 EKDKHYKMVYQLEKKYYPDQCLDKAFLQPGEVLKKSDDSGKVR-GKKKVVAALGGKRSEV 249

Query: 259 PN-GM-----NDKGLVFFDEGRPEKENKGSMKD---VAEKKVEEFFKCLKKVP---GKDS 318
              GM     +D  LVFFDE + ++E K S  D   V EKKVE+FFK L K P   G  S
Sbjct: 250 KRIGMENCDEDDDKLVFFDEVKEKEEKKKSEDDVVVVTEKKVEQFFKGLTKSPNEKGMAS 309

Query: 319 EIGQTEPYLLTRHTELPAKWDSPCGTVVLLNKPK--------------------GHAGTL 378
             G  EP+L+TR+ ELP +WD P GTV+L+NKPK                    GHAGTL
Sbjct: 310 GGGDGEPFLVTRNGELPPRWDGPNGTVLLVNKPKGWTSFTVCGKLRRLVKVKKVGHAGTL 369

Query: 379 DPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSPVIQREPWEHIKDD 438
           DPMATGLLIVCVGKATK+VDRYQGMIK YSGVFRLGEATST DADSPVIQRE WEHIKDD
Sbjct: 370 DPMATGLLIVCVGKATKVVDRYQGMIKGYSGVFRLGEATSTLDADSPVIQRESWEHIKDD 429

Query: 439 DIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRISIFKFDIERSLDD 498
           DIKKA  SF GEIWQVPPMFSAIKVGGEKMYEKARRGE++ELSPRRISIF+FDIERSLDD
Sbjct: 430 DIKKALTSFLGEIWQVPPMFSAIKVGGEKMYEKARRGETVELSPRRISIFQFDIERSLDD 489

Query: 499 RQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRRDSIGQYLADDAWEFKELEDAIT 526
           RQNLIFRV CSKGTYIRSLCADL K LGSCAHLTALRRDSIG+Y A+DAWEF ELE AIT
Sbjct: 490 RQNLIFRVICSKGTYIRSLCADLAKALGSCAHLTALRRDSIGEYSANDAWEFNELEAAIT 540

[Close]
BLAST of Lsi02G016040 vs. TAIR 10
Match: AT3G57150.1 (homologue of NAP57 )

HSP 1 Score: 89.0 bits (219), Expect = 1.3e-17
Identity = 59/171 (34.50%), Postives = 86/171 (50.29%), Query Frame = 0 

Query: 329 VLLNKPKGHAGTLDPMATGLLIVCVGKATKLVDRYQGMIKSYSGVFRLGEATSTWDADSP 388
           +L  +  GH+GTLDP  TG LIVC+ +AT+LV   QG  K Y  V RL  A        P
Sbjct: 103 ILRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQGAGKEYVCVARLHSAV-------P 162

Query: 389 VIQREPWEHIKDDDIKKAAASFCGEIWQVPPMFSAIKVGGEKMYEKARRGESIELSPRRI 448
            + +          + +A  S  G ++Q PP+ SA+K        +  R  +I  S    
Sbjct: 163 DVAK----------VARALESLTGAVFQRPPLISAVK--------RQLRIRTIYES---- 222

Query: 449 SIFKFDIERSLDDRQNLIFRVTCSKGTYIRSLCADLGKVLGSCAHLTALRR 500
            + ++D +R L     ++F V+C  GTYIR++C  LG +LG   H+  LRR
Sbjct: 223 KLLEYDADRHL-----VVFWVSCEAGTYIRTMCVHLGLLLGVGGHMQELRR 239

The following BLAST results are available for this feature:
BLAST of Lsi02G016040 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9RB364.9e-4345.33tRNA pseudouridine synthase B OS=Flavobacterium johnsoniae (strain ATCC 17061 / ... [more]
A6LEL71.6e-3843.72tRNA pseudouridine synthase B OS=Parabacteroides distasonis (strain ATCC 8503 / ... [more]
A0JUU84.8e-3842.86tRNA pseudouridine synthase B OS=Arthrobacter sp. (strain FB24) OX=290399 GN=tru... [more]
A3PY884.8e-3845.41tRNA pseudouridine synthase B OS=Mycobacterium sp. (strain JLS) OX=164757 GN=tru... [more]
A1UET14.8e-3845.41tRNA pseudouridine synthase B OS=Mycobacterium sp. (strain KMS) OX=189918 GN=tru... [more]
BLAST of Lsi02G016040 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3C8B06.3e-27588.62uncharacterized protein LOC103498118 OS=Cucumis melo OX=3656 GN=LOC103498118 PE=... [more]
A0A0A0KP031.9e-27187.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G522940 PE=3 SV=1[more]
A0A6J1JSH82.1e-27087.71uncharacterized protein LOC111489372 OS=Cucurbita maxima OX=3661 GN=LOC111489372... [more]
A0A6J1FDZ77.4e-26886.97uncharacterized protein LOC111444875 OS=Cucurbita moschata OX=3662 GN=LOC1114448... [more]
A0A5A7T7S02.1e-26288.19TruB_N domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
BLAST of Lsi02G016040 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038891059.17.3e-27889.54uncharacterized protein LOC120080471 [Benincasa hispida][more]
XP_008458830.11.3e-27488.62PREDICTED: uncharacterized protein LOC103498118 [Cucumis melo][more]
XP_011655389.23.9e-27187.71uncharacterized protein LOC101215528 isoform X1 [Cucumis sativus] >KGN51348.1 hy... [more]
XP_022993331.14.3e-27087.71uncharacterized protein LOC111489372 [Cucurbita maxima] >XP_022993332.1 uncharac... [more]
XP_023550635.11.8e-26887.16uncharacterized protein LOC111808717 [Cucurbita pepo subsp. pepo][more]
BLAST of Lsi02G016040 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G14460.19.2e-17063.05Pseudouridine synthase family protein [more]
AT3G57150.11.3e-1734.50homologue of NAP57 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014780tRNA pseudouridine synthase II, TruBTIGRFAMTIGR00431TIGR00431coord: 333..516
e-value: 3.0E-55
score: 185.1
IPR014780tRNA pseudouridine synthase II, TruBPANTHERPTHR13767TRNA-PSEUDOURIDINE SYNTHASEcoord: 336..523
IPR014780tRNA pseudouridine synthase II, TruBHAMAPMF_01080TruB_bactcoord: 293..525
score: 13.369517
IPR014780tRNA pseudouridine synthase II, TruBCDDcd02573PseudoU_synth_EcTruBcoord: 328..519
e-value: 2.95549E-87
score: 265.847
IPR002501Pseudouridine synthase II, N-terminalPFAMPF01509TruB_Ncoord: 335..477
e-value: 1.2E-48
score: 165.3
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 325..524
e-value: 6.9E-74
score: 250.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..106
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..106
IPR032819tRNA pseudouridylate synthase B, C-terminalPFAMPF16198TruB_C_2coord: 478..521
e-value: 7.0E-10
score: 38.9
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 327..519

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G016040.1Lsi02G016040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990481 mRNA pseudouridine synthesis
biological_process GO:0006400 tRNA modification
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
biological_process GO:0006396 RNA processing
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding