Lsi02G015590 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G015590
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCASP-like protein
Locationchr02: 21444799 .. 21447542 (+)
RNA-Seq ExpressionLsi02G015590
SyntenyLsi02G015590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGATGTAATCTAGCCTCACTTTCCTCCAACTTATCATTAAACTAAACACAAGGTTCGTGCTCATTGTAGGACTTAACCCAACACCTAACGCTAGAAATTAACAACCTGACGACGTTCTTCGCTTTGCATTGATCCAGCTCGGTCAGACAATAATAAAACTCTTTACTTAGAGCGTAGAGGGCAGTTCTTGTAATTTTTTGCAAGTTTTATGTAAATATTTTCTAAAATTAGGGCAATATGTAAATACTAAATAAAATTGTAGATAAAACCAAACATCAAACTTCCAAGGAGAAAAATTAGAGAGATTGGTCTAAAAATATTCTTCCAGTTAGAGAGAGAGTCAGTCACCAAGTACAGCCTCCATGGCAGCATCAAACTCTGAAGCAGAAATGAAAAAGAAAGAACTAGCAAAAACAATGGAAGAAGAAGAAGAAGAAGAAAGACAGCAACATCACCAGAATCAATCAGAGGAAAACGAATCCCATCCACCTCTCTCTGCTCGCTCTTCACCTCCCCATTCCCTCTCCAGCAGCAAAGATCCCTCGCCGCTGCTCCACTCTCCGTCCATCTCCTCGGATTTTTCTGATCATACTTGTCATACTCATGGAAACTCCTCCATCGACGACGATCTCTCCATTACACGGCTTGAGGACTTGCCACAGCCGGCTAATCCTCCGTCTCCCAGACCTGTGGCGGCCAACCGCGCGCAGCCGACGGAGCCGATTGTGGTGACCAAGGTGGATGCTGAAATACAGGGAGTACGGAAAGTGGAAGAGGTAAGTGATGGTGATGGTGATGGCGATGGCGATGGCGATGTAGAGAGTCGCGATAGCGGTAAGGTTGGGCGAGGACGGAAGCTGATGGCGAGTTTGTCGATAAAAAAAATAAAGAGGGAGGAATTGAGGAAGAAGATCTTGCTAGGGTTTAGAGTTTGTGGATTCGCCTTCTGTTTGGTTTCGTTTTCGGTTATGGCTTCTGACAAGAATCAGGGCTGGGCTTTGGATTCATTCTATCGCTACAAGGAATTCAGGTATGTTGAATGAAATCACTTCTTCCTATTGAAATTTCGTATGAAACTTCCTTAACTCGAATTGAATTGGATATCGCTCGATTCTTTCTATTGCTCAAGTCATAATCTCTCTGTTACTATTAGTATTCAATAAAAAAATTTCTTCTGGAATTGGAAATTTCATCCAATTTGCAGGTACAGTATGGCAGTGAATGTTATTGGGTTTGTTTACTCTGCACTTCAATCCTATGATCTTGTATATTTCCTCTCCACTGGGAAACACATGATCCGTAATCACTTCAAGCAATACTTCGATTTCTTCATTGATCAGGCAAGTTTCTGTTTGTCTTGAAATTGGGTTCCTGGGTTTCAATCTGTTAATTATTAATTCTGTGATAATCTCAGTACCTAACCCAAGCACATATTGGATGGCCAAGATCAGGCCAATATTTTAGTCCAGAATTCAGATGACATTATTGTGTATCGATTATGATAGCTTATAGGACCCACAGAAGATAAAAAGGTGATGCATAATTCTCAATAGTCACTAGATAGATGGTTCTCTTACTGTATTCCTTTTCTGAAATTTTTGCCATTCATAATGAAGCTTTATCTTACCTTTTTCGTACAGAAGAATCTGCAAGCTCTTCTCATGAAGAACTTACTAATACTTTTACCTTAAATCCTCTCAATTCGCATATTAATTACACAGTAGTAGTTGGAAAAACTTGGATTGGTTTTGTTGAGTAACTTTCCTTTTATTCTTTCCTTTTTATTTGCCTTAGCTTTTGGGGCATCCTTTTTGTTACCTTTTCTCTGGTAATGCCGCCTTTCCCTCGCATCTTTTTTATTTTTCCTTTTTATTCTTTAAAGTTTATAAGGAAGCTGGCTTTAATTTGCAGCAAAGAATCTATATAGAAATTATCACTTGGTTTTTAAAAGAAAATAACTTCTGACCATAACTAGCATAAGCTAGAAAAAAGGTATTTTCCTTTAAGTTGCAAAATCATTGCAATTAAATCTGACACTTACACTTTTATAAGTGGTTGAGCTTTAAGTCATCGATTAAAAAAATTCAATGGTGTAAGTGCAAAACAACCGGCTTGTCATTTAAGAATGATTTAAGGTTTGGAATTGAAGTGTAACAACCTCGAACCTTGTAGTTAGACGGAGGCTGCACATTTTTCAAGTTGCAACTATATGGAGTTAGGATGACTGAGTTTAATAATTGCTTAAACCGAAAATGCTAAGATGCAATAACTCTTATAGTCAGAGGTTTTTGCATTTCGTAGGCAAAACCTGAACTTTATGGATAGAACTGAGTCATGATGCAAACATTCAATTGCATATGTATGAAGTTATTAGTACTTTTGATTAATGTTTGAGTTTGAGGTTGGCTACATTTGGTGATAAGCTCCAAGAAATTGATGACAGTATTGTTCTGTTGCAGATAATAGCATATCTTCTTCTATCAGCTTCGTCTTCGGCCGCCACCCGAATTGATGATTGGCAATCCAATTGGGGAAAGGACAAGTTTCCAGATATGGCTACCACGTCTTTGGGATTGTCCATTGTTGCATTTGTAGCTTTTGCCTTGAGCTGTATAATCTCTGGTTATACTCTCTGTAAATCTGCTTAGGTAACATTTCTGGACTTGCTTTTTGGCAAACTATTCATGATGATAGCAACAAACAAACCAATGCTGGTGAGCATATACAAGAAAATTTATTTC

mRNA sequence

AAAGGATGTAATCTAGCCTCACTTTCCTCCAACTTATCATTAAACTAAACACAAGGTTCGTGCTCATTGTAGGACTTAACCCAACACCTAACGCTAGAAATTAACAACCTGACGACGTTCTTCGCTTTGCATTGATCCAGCTCGGTCAGACAATAATAAAACTCTTTACTTAGAGCGTAGAGGGCAGTTCTTGTAATTTTTTGCAAGTTTTATGTAAATATTTTCTAAAATTAGGGCAATATGTAAATACTAAATAAAATTGTAGATAAAACCAAACATCAAACTTCCAAGGAGAAAAATTAGAGAGATTGGTCTAAAAATATTCTTCCAGTTAGAGAGAGAGTCAGTCACCAAGTACAGCCTCCATGGCAGCATCAAACTCTGAAGCAGAAATGAAAAAGAAAGAACTAGCAAAAACAATGGAAGAAGAAGAAGAAGAAGAAAGACAGCAACATCACCAGAATCAATCAGAGGAAAACGAATCCCATCCACCTCTCTCTGCTCGCTCTTCACCTCCCCATTCCCTCTCCAGCAGCAAAGATCCCTCGCCGCTGCTCCACTCTCCGTCCATCTCCTCGGATTTTTCTGATCATACTTGTCATACTCATGGAAACTCCTCCATCGACGACGATCTCTCCATTACACGGCTTGAGGACTTGCCACAGCCGGCTAATCCTCCGTCTCCCAGACCTGTGGCGGCCAACCGCGCGCAGCCGACGGAGCCGATTGTGGTGACCAAGGTGGATGCTGAAATACAGGGAGTACGGAAAGTGGAAGAGGTAAGTGATGGTGATGGTGATGGCGATGGCGATGGCGATGTAGAGAGTCGCGATAGCGGTAAGGTTGGGCGAGGACGGAAGCTGATGGCGAGTTTGTCGATAAAAAAAATAAAGAGGGAGGAATTGAGGAAGAAGATCTTGCTAGGGTTTAGAGTTTGTGGATTCGCCTTCTGTTTGGTTTCGTTTTCGGTTATGGCTTCTGACAAGAATCAGGGCTGGGCTTTGGATTCATTCTATCGCTACAAGGAATTCAGGTACAGTATGGCAGTGAATGTTATTGGGTTTGTTTACTCTGCACTTCAATCCTATGATCTTGTATATTTCCTCTCCACTGGGAAACACATGATCCGTAATCACTTCAAGCAATACTTCGATTTCTTCATTGATCAGGCAAAAGAATCTGCAAGCTCTTCTCATGAAGAACTTACTAATACTTTTACCTTAAATCCTCTCAATTCGCATATTAATTACACAGTAATAATAGCATATCTTCTTCTATCAGCTTCGTCTTCGGCCGCCACCCGAATTGATGATTGGCAATCCAATTGGGGAAAGGACAAGTTTCCAGATATGGCTACCACGTCTTTGGGATTGTCCATTGTTGCATTTGTAGCTTTTGCCTTGAGCTGTATAATCTCTGGTTATACTCTCTGTAAATCTGCTTAGGTAACATTTCTGGACTTGCTTTTTGGCAAACTATTCATGATGATAGCAACAAACAAACCAATGCTGGTGAGCATATACAAGAAAATTTATTTC

Coding sequence (CDS)

ATGGCAGCATCAAACTCTGAAGCAGAAATGAAAAAGAAAGAACTAGCAAAAACAATGGAAGAAGAAGAAGAAGAAGAAAGACAGCAACATCACCAGAATCAATCAGAGGAAAACGAATCCCATCCACCTCTCTCTGCTCGCTCTTCACCTCCCCATTCCCTCTCCAGCAGCAAAGATCCCTCGCCGCTGCTCCACTCTCCGTCCATCTCCTCGGATTTTTCTGATCATACTTGTCATACTCATGGAAACTCCTCCATCGACGACGATCTCTCCATTACACGGCTTGAGGACTTGCCACAGCCGGCTAATCCTCCGTCTCCCAGACCTGTGGCGGCCAACCGCGCGCAGCCGACGGAGCCGATTGTGGTGACCAAGGTGGATGCTGAAATACAGGGAGTACGGAAAGTGGAAGAGGTAAGTGATGGTGATGGTGATGGCGATGGCGATGGCGATGTAGAGAGTCGCGATAGCGGTAAGGTTGGGCGAGGACGGAAGCTGATGGCGAGTTTGTCGATAAAAAAAATAAAGAGGGAGGAATTGAGGAAGAAGATCTTGCTAGGGTTTAGAGTTTGTGGATTCGCCTTCTGTTTGGTTTCGTTTTCGGTTATGGCTTCTGACAAGAATCAGGGCTGGGCTTTGGATTCATTCTATCGCTACAAGGAATTCAGGTACAGTATGGCAGTGAATGTTATTGGGTTTGTTTACTCTGCACTTCAATCCTATGATCTTGTATATTTCCTCTCCACTGGGAAACACATGATCCGTAATCACTTCAAGCAATACTTCGATTTCTTCATTGATCAGGCAAAAGAATCTGCAAGCTCTTCTCATGAAGAACTTACTAATACTTTTACCTTAAATCCTCTCAATTCGCATATTAATTACACAGTAATAATAGCATATCTTCTTCTATCAGCTTCGTCTTCGGCCGCCACCCGAATTGATGATTGGCAATCCAATTGGGGAAAGGACAAGTTTCCAGATATGGCTACCACGTCTTTGGGATTGTCCATTGTTGCATTTGTAGCTTTTGCCTTGAGCTGTATAATCTCTGGTTATACTCTCTGTAAATCTGCTTAG

Protein sequence

MAASNSEAEMKKKELAKTMEEEEEEERQQHHQNQSEENESHPPLSARSSPPHSLSSSKDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKREELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTVIIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLCKSA
Homology
BLAST of Lsi02G015590 vs. ExPASy Swiss-Prot
Match: Q84WP5 (CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.5e-38
Identity = 118/321 (36.76%), Postives = 168/321 (52.34%), Query Frame = 0

Query: 42  PPLSARSSPPHSLSSSKDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLS-ITRLEDLPQ 101
           P  +  S  P S+S+ K P P   S +I + F + T H   + S    L   +  E    
Sbjct: 4   PAKTMPSMSPSSVSTEKSPPPSDTSMAIVA-FDNSTTHFSSSPSPPHSLDHSSESEKEDA 63

Query: 102 PANPPSPRPVAANRAQPT-EPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGK 161
            + P S R     + + T  PIVV          R V+EV            V +R S +
Sbjct: 64  KSKPESRRNKNPGKVEETPSPIVVVH-----NHNRSVKEV------------VPTRKSAR 123

Query: 162 VGRGRKL-----MASLSIKKIKREELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALD 221
           VG GR         S  +++ +REE+ K   LGFR+      L+SFS+MA+DK +GW+ D
Sbjct: 124 VGSGRSSGQRSGAVSAILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGD 183

Query: 222 SFYRYKEFRYSMAVNVIGFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESAS 281
           SF RYKE+R+ ++VNV+ FVYS+ Q+ DL Y L   KH+I +H +  F+F IDQ      
Sbjct: 184 SFDRYKEYRFCLSVNVVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQ------ 243

Query: 282 SSHEELTNTFTLNPLNSHINYTVIIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSL 341
                                  ++AYLL+SAS++A TR+DDW SNWGKD+F +MA+ S+
Sbjct: 244 -----------------------VLAYLLMSASTAAVTRVDDWVSNWGKDEFTEMASASI 277

Query: 342 GLSIVAFVAFALSCIISGYTL 356
            +S +AF+AFA S +ISGY L
Sbjct: 304 AMSFLAFLAFAFSSLISGYNL 277

BLAST of Lsi02G015590 vs. ExPASy Swiss-Prot
Match: D7LIR2 (CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_482607 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 6.2e-37
Identity = 112/317 (35.33%), Postives = 169/317 (53.31%), Query Frame = 0

Query: 48  SSPPHSLSSSKDPSPLLHSPSISSDFSDHTCHTHGN----SSIDDDLSITRLEDLPQPAN 107
           S  P S+S+ K P P   S +I + F + T H   +     S+D      + ++  +P +
Sbjct: 3   SMSPSSISTEKSPPPSDTSMAIVA-FDNSTTHLSSSPSPPHSLDHSSDSEKEDEKRRPES 62

Query: 108 PPSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRG 167
             +  PV     +   PIVV          R V+EV            V +R + +VG G
Sbjct: 63  RRNKNPVKIE--ETPSPIVVVH-----NHNRSVKEV------------VPTRKTARVGSG 122

Query: 168 R----KLMASLSI-KKIKREELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYR 227
           R    +  A L+I ++ +REE+ K + LGFR+      L+SFS+MA+DK +GW+ DSF R
Sbjct: 123 RSSGQRSGAVLAILRRSRREEIVKFVALGFRLSEVVLALISFSIMAADKTKGWSGDSFDR 182

Query: 228 YKEFRYSMAVNVIGFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHE 287
           YKE+R+ ++VNV+ F+Y++ Q+ DL Y L   KH+I +H +  F+F IDQ          
Sbjct: 183 YKEYRFCLSVNVVAFIYASFQACDLAYHLVKEKHLISHHLRPLFEFIIDQ---------- 242

Query: 288 ELTNTFTLNPLNSHINYTVIIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSI 347
                              ++AYLL+ AS++A TR+DDW SNWGKD F +MA+ S+ +S 
Sbjct: 243 -------------------VLAYLLMCASTAAVTRVDDWVSNWGKDDFTEMASASIAMSF 270

Query: 348 VAFVAFALSCIISGYTL 356
           + F+AFA S +ISGY L
Sbjct: 303 LTFLAFAFSSLISGYNL 270

BLAST of Lsi02G015590 vs. ExPASy Swiss-Prot
Match: Q9FNE8 (CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.2e-34
Identity = 124/344 (36.05%), Postives = 176/344 (51.16%), Query Frame = 0

Query: 13  KELAKTMEEEEEEERQQHHQNQSEENESHPPLSARSSPPHSLSSSKDPSPLLHSPSISSD 72
           +EL KT + ++++++QQ  Q+QS  +  +  +S+RSS  HSL  +   SP   SP++SS 
Sbjct: 2   EELEKTQKFQKKKKQQQEKQDQS--SPINFEMSSRSS-LHSLPQTTIESP-PDSPTLSS- 61

Query: 73  FSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQPTEPIVVTKVDAEIQG 132
                  +HG+                      SP  +      PT  +  T+    +  
Sbjct: 62  ----IPDSHGS----------------------SPHTII-----PTPSVAKTETPFRVTN 121

Query: 133 VRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKREELRKKILLGFRVCG 192
             + ++VS               +S +  R     +S + ++ K   L +K LLGFRV  
Sbjct: 122 GEEEKKVS---------------ESRRQLRPSFSSSSSTPRESKWASLIRKALLGFRVIA 181

Query: 193 FAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSALQSYDLVYFLSTGKH 252
           F  CLVSFSVM SD+++GWA DSFY YKEFR+ +A NVIGFVYS     DLVY LST   
Sbjct: 182 FVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVYSGFMICDLVYLLSTSIR 241

Query: 253 MIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTVIIAYLLLSASSSAAT 312
             R++ + + +F +DQ                             ++AYLL SAS+SA+ 
Sbjct: 242 RSRHNLRHFLEFGLDQ-----------------------------MLAYLLASASTSASI 265

Query: 313 RIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLC 357
           R+DDWQSNWG DKFPD+A  S+ LS V+FVAFA   + SGY LC
Sbjct: 302 RVDDWQSNWGADKFPDLARASVALSYVSFVAFAFCSLASGYALC 265

BLAST of Lsi02G015590 vs. ExPASy Swiss-Prot
Match: D7MMW4 (CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_919556 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.5e-30
Identity = 107/331 (32.33%), Postives = 160/331 (48.34%), Query Frame = 0

Query: 26  ERQQHHQNQSEENESHPPLSARSSPPHSLSSSKDPSPL-LHSPSISSDFSDHTCHTHGNS 85
           E   ++++       H PLS     P S   S + SP  + +P                 
Sbjct: 12  EAYSYNESPHSPLRFHSPLSDAGDLPESRYVSPEGSPFKIENP----------------K 71

Query: 86  SIDDDLSITRLEDLPQPANPPSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEVSDGDG 145
           SI     +T+   LP P  PP P+     R +     + + +D     +         DG
Sbjct: 72  SIVAGNKLTQFSPLPSPIPPPPPQIPPPRRQRNARVPMNSSLDKSPSSMVVQNSWVREDG 131

Query: 146 DGDGDGDVESRDSGKVGRGRKLMASLSIKKIKREELRKKILLGFRVCGFAFCLVSFSVMA 205
             +      +R +G    G +  A+ ++ + +R++L     LGFR+     C++SFS+MA
Sbjct: 132 QQN-----TTRKAGAPMNGEE-SATTAVNRARRDDLVSVTALGFRITEVILCVISFSIMA 191

Query: 206 SDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDF 265
           +DK QGW+ DS+ RYKE+RY +AVNVI FVYSA ++ D   +++   +M+   F   F F
Sbjct: 192 ADKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYMAKESYMMNCGFHDLFVF 251

Query: 266 FIDQAKESASSSHEELTNTFTLNPLNSHINYTVIIAYLLLSASSSAATRIDDWQSNWGKD 325
            +DQ                             ++AYLL+SASS AATR+DDW SNWGKD
Sbjct: 252 SMDQ-----------------------------LLAYLLMSASSCAATRVDDWVSNWGKD 291

Query: 326 KFPDMATTSLGLSIVAFVAFALSCIISGYTL 356
           +F  MAT S+ +S +AF AFA+S +IS Y L
Sbjct: 312 EFTQMATASIAVSFLAFGAFAVSALISSYRL 291

BLAST of Lsi02G015590 vs. ExPASy Swiss-Prot
Match: Q501G6 (CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.8e-29
Identity = 112/347 (32.28%), Postives = 162/347 (46.69%), Query Frame = 0

Query: 10  MKKKELAKTMEEEEEEERQQHHQNQSEENESHPPLSARSSPPHSLSSSKDPSPL-LHSPS 69
           MK K  A +       E Q ++++       H PLS     P S   S + SP  + +P 
Sbjct: 1   MKMKRTASS-----NSEAQSYNESPHSPLRFHSPLSDAGDLPESRYVSPEGSPFKIENP- 60

Query: 70  ISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQPTEPIVVTKVDA 129
                           SI     +T+   LP P  PP P+     R +     + +  D 
Sbjct: 61  ---------------KSIVAGNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSSSDK 120

Query: 130 EIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKREELRKKILLGF 189
               +         DG     G   +R +G    G +     ++ + + ++L     LGF
Sbjct: 121 SPSSMVVHNSWVREDG-----GQTTTRKAGAPINGEE-STRTTVNRARGDDLVSLTALGF 180

Query: 190 RVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSALQSYDLVYFLS 249
           R+     C++SFS+MA+DK QGW+ DS+ RYKE+RY +AVNVI FVYSA ++ D   +++
Sbjct: 181 RITEVILCVISFSIMAADKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIA 240

Query: 250 TGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTVIIAYLLLSASS 309
              +MI   F   F F +DQ                             ++AYLL+SASS
Sbjct: 241 KESYMINCGFHDLFVFSMDQ-----------------------------LLAYLLMSASS 291

Query: 310 SAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTL 356
            AATR+DDW SNWGKD+F  MAT S+ +S +AF AFA+S +IS Y L
Sbjct: 301 CAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVSALISSYRL 291

BLAST of Lsi02G015590 vs. ExPASy TrEMBL
Match: A0A5A7T7H8 (CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00890 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.1e-132
Identity = 277/362 (76.52%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 2   AASNSEAEMKKKELAKTMEEEEEEERQQHHQNQSEE----NESHPPLSARSSPPHSLSSS 61
           +ASNSEAEMK++EL K+MEE +E+  +Q H NQS+E    NESH  LSA SSPPHSLS+S
Sbjct: 4   SASNSEAEMKREELPKSMEEPQEQVHEQPHHNQSDEGENDNESHLHLSACSSPPHSLSTS 63

Query: 62  KDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQP 121
           KDPSP LHSPSISSDFSDHTCH+ GNSSIDD      ++DLP  ANPPSPRPVAANRAQP
Sbjct: 64  KDPSPPLHSPSISSDFSDHTCHSRGNSSIDD------VQDLPPSANPPSPRPVAANRAQP 123

Query: 122 TEPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKR 181
           +EPIVVTKVD EIQGVRKVEEVS      D DGDVES D GKVGRGRKLM SLSIKKIK 
Sbjct: 124 SEPIVVTKVDTEIQGVRKVEEVS------DSDGDVESADGGKVGRGRKLMESLSIKKIKE 183

Query: 182 EELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSA 241
           EELRKKILLGFR+CGF+FCLVS SVMASDK+QGWALDSFYRYKEFRY MAVNVIGFVYSA
Sbjct: 184 EELRKKILLGFRICGFSFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSA 243

Query: 242 LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTV 301
           LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ                             
Sbjct: 244 LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ----------------------------- 303

Query: 302 IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLCK 360
           IIAYLLLSASSSAATRIDDWQSNWG+DKFPDMAT SL LSIVAFVAFALSCIISGYTLCK
Sbjct: 304 IIAYLLLSASSSAATRIDDWQSNWGRDKFPDMATGSLALSIVAFVAFALSCIISGYTLCK 324

BLAST of Lsi02G015590 vs. ExPASy TrEMBL
Match: A0A1S3C844 (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103498066 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.1e-132
Identity = 277/362 (76.52%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 2   AASNSEAEMKKKELAKTMEEEEEEERQQHHQNQSEE----NESHPPLSARSSPPHSLSSS 61
           +ASNSEAEMK++EL K+MEE +E+  +Q H NQS+E    NESH  LSA SSPPHSLS+S
Sbjct: 4   SASNSEAEMKREELPKSMEEPQEQVHEQPHHNQSDEGENDNESHLHLSACSSPPHSLSTS 63

Query: 62  KDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQP 121
           KDPSP LHSPSISSDFSDHTCH+ GNSSIDD      ++DLP  ANPPSPRPVAANRAQP
Sbjct: 64  KDPSPPLHSPSISSDFSDHTCHSRGNSSIDD------VQDLPPSANPPSPRPVAANRAQP 123

Query: 122 TEPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKR 181
           +EPIVVTKVD EIQGVRKVEEVS      D DGDVES D GKVGRGRKLM SLSIKKIK 
Sbjct: 124 SEPIVVTKVDTEIQGVRKVEEVS------DSDGDVESADGGKVGRGRKLMESLSIKKIKE 183

Query: 182 EELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSA 241
           EELRKKILLGFR+CGF+FCLVS SVMASDK+QGWALDSFYRYKEFRY MAVNVIGFVYSA
Sbjct: 184 EELRKKILLGFRICGFSFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSA 243

Query: 242 LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTV 301
           LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ                             
Sbjct: 244 LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ----------------------------- 303

Query: 302 IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLCK 360
           IIAYLLLSASSSAATRIDDWQSNWG+DKFPDMAT SL LSIVAFVAFALSCIISGYTLCK
Sbjct: 304 IIAYLLLSASSSAATRIDDWQSNWGRDKFPDMATGSLALSIVAFVAFALSCIISGYTLCK 324

BLAST of Lsi02G015590 vs. ExPASy TrEMBL
Match: A0A0A0KS01 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G630810 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 4.5e-131
Identity = 275/362 (75.97%), Postives = 295/362 (81.49%), Query Frame = 0

Query: 2   AASNSEAEMKKKELAKTMEEEEEEERQQHHQNQ--SEENESHPPLSARSSPPHSLSSSKD 61
           +ASNSEAEMKK+EL K+MEE  E+E   H+Q+    ++NESH  LSA SSPPHSLS++KD
Sbjct: 6   SASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDNESHLHLSACSSPPHSLSTNKD 65

Query: 62  PSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLED--LPQPANPPSPRPVAANRAQP 121
           PSP LHSPSISSDFSDHTCH+ GNSSID  LSIT L+D  LP  AN PSPRPVAANRAQP
Sbjct: 66  PSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAANRAQP 125

Query: 122 TEPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKR 181
           +EPIVVTKVD EIQG+RKVEEV D DGD +  GD +  D GKVGRGRKLM +LSIKKIKR
Sbjct: 126 SEPIVVTKVDTEIQGIRKVEEVIDSDGDVE-SGDGDDGDGGKVGRGRKLMETLSIKKIKR 185

Query: 182 EELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSA 241
           EELRKKILLGFR+CGFAFCLVS SVMASDK+QGWALDSFYRYKEFRY MAVNVIGFVYSA
Sbjct: 186 EELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSA 245

Query: 242 LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTV 301
           LQSYDLVYFLSTGKH+IRNHFKQYFDFFIDQ                             
Sbjct: 246 LQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ----------------------------- 305

Query: 302 IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLCK 360
           IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMAT SLG SIVAFVAFALSCIISGYTLCK
Sbjct: 306 IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATGSLGFSIVAFVAFALSCIISGYTLCK 337

BLAST of Lsi02G015590 vs. ExPASy TrEMBL
Match: A0A6J1FJD7 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444795 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.9e-130
Identity = 272/363 (74.93%), Postives = 293/363 (80.72%), Query Frame = 0

Query: 1   MAASNSEAEMKKKELAKTMEEEEEEERQQHHQNQS---EENESHPPLSARSSPPHSLSSS 60
           MAASNSEAE+KK+ELA+TME+++     Q   N++    ++ESHPPLSA+SSP HSLS +
Sbjct: 1   MAASNSEAEVKKEELAETMEQQQLHRHSQSEVNENGNENDDESHPPLSAQSSPAHSLSDN 60

Query: 61  KDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQP 120
           KDPSP LHSPS SSDFSDHTC THGNSSIDD LSITRLEDLP  A PPSPRPVAANRAQP
Sbjct: 61  KDPSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLEDLPPSAYPPSPRPVAANRAQP 120

Query: 121 TEPIVVTKVDAEIQGVRKVEEVSDGDGD-GDGDGDVESRDSGKVGRGRKLMASLSIKKIK 180
           TEPIVVTKVDAEIQGVRKVEE S  D +  DGDG       G VGRGRKLMA+LS+KKIK
Sbjct: 121 TEPIVVTKVDAEIQGVRKVEEGSASDVEPSDGDG-------GPVGRGRKLMANLSMKKIK 180

Query: 181 REELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYS 240
           REELRKKILLGFR+ GF FCL SFSVMASDKNQGWALDSFYRYKEFRY MAVNVIGFVYS
Sbjct: 181 REELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYS 240

Query: 241 ALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYT 300
           ALQSYDL+YFLSTGKHMIRN+FKQYFDFFIDQ                            
Sbjct: 241 ALQSYDLIYFLSTGKHMIRNYFKQYFDFFIDQ---------------------------- 300

Query: 301 VIIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLC 360
            I+AYLLLSASSSAATR+DDWQSNWG DKFPDMA TS+GLSIVAFVAFALSCIISGYTLC
Sbjct: 301 -IVAYLLLSASSSAATRVDDWQSNWGMDKFPDMAVTSVGLSIVAFVAFALSCIISGYTLC 327

BLAST of Lsi02G015590 vs. ExPASy TrEMBL
Match: A0A6J1JTE4 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489567 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 3.5e-128
Identity = 270/365 (73.97%), Postives = 293/365 (80.27%), Query Frame = 0

Query: 1   MAASNSEAEMKKKELAKTMEEEEEEERQQHHQNQSE-----ENESHPPLSARSSPPHSLS 60
           MAASNSEAE+KK+ELA+TME+++     Q   N++E     ++ESHPPLSA+SSPPHSLS
Sbjct: 1   MAASNSEAEVKKEELAETMEQQQLHRHNQSEVNENENENENDDESHPPLSAQSSPPHSLS 60

Query: 61  SSKDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRA 120
            SKDPSP LHSPS +SDFSDHTC THGNSSIDD LSITRLEDL   + PPSPRPVAANRA
Sbjct: 61  DSKDPSPPLHSPSNASDFSDHTCQTHGNSSIDDALSITRLEDLSPSSYPPSPRPVAANRA 120

Query: 121 QPTEPIVVTKVDAEIQGVRKVEEVSDGDGD-GDGDGDVESRDSGKVGRGRKLMASLSIKK 180
           QP+EPIVVTKVDAEIQGVRKVEE S  D +  DGDG       G VGRGRKLMA+LS+KK
Sbjct: 121 QPSEPIVVTKVDAEIQGVRKVEEGSASDVEPSDGDG-------GPVGRGRKLMANLSMKK 180

Query: 181 IKREELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFV 240
           IKREELRKKILLGFR+ GF FCL SFSVMASDKNQGWALDSFYRYKEFRY MAVNVIGFV
Sbjct: 181 IKREELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFV 240

Query: 241 YSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHIN 300
           YSALQS+DLVYFLSTGK MIRN+FKQYFDFFIDQ                          
Sbjct: 241 YSALQSFDLVYFLSTGKQMIRNYFKQYFDFFIDQ-------------------------- 300

Query: 301 YTVIIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYT 360
              I+AYLLLSASSSAATR+DDWQSNWG DKFPDMA TS+GLSIVAFVAFALSCIISGYT
Sbjct: 301 ---IVAYLLLSASSSAATRVDDWQSNWGMDKFPDMAITSVGLSIVAFVAFALSCIISGYT 329

BLAST of Lsi02G015590 vs. NCBI nr
Match: XP_038890250.1 (CASP-like protein 4A1 [Benincasa hispida])

HSP 1 Score: 537.3 bits (1383), Expect = 9.8e-149
Identity = 304/368 (82.61%), Postives = 312/368 (84.78%), Query Frame = 0

Query: 1   MAASNSEAEMKKKELAKTMEEEEEEERQQ-----HHQNQSEEN----ESHPPLSARSSPP 60
           MAAS+SEAEMKK+E AKTMEEEEEE++QQ     HH NQSEEN    ESHPPLSARSSPP
Sbjct: 1   MAASSSEAEMKKEEQAKTMEEEEEEQQQQQQQQKHHHNQSEENENDDESHPPLSARSSPP 60

Query: 61  HSLSSSKDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVA 120
           HSLSSSKDPSP LHSPSISSDFSDHTCHTHGNSSIDD LSITRLEDLP  ANPPSPRPVA
Sbjct: 61  HSLSSSKDPSPPLHSPSISSDFSDHTCHTHGNSSIDDALSITRLEDLPPSANPPSPRPVA 120

Query: 121 ANRAQPTEPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLS 180
           ANRAQPTEPIVVTKVDAEIQGVRKVEEVS      D DGDVES D GKVGRGRKLMASLS
Sbjct: 121 ANRAQPTEPIVVTKVDAEIQGVRKVEEVS------DSDGDVESGDGGKVGRGRKLMASLS 180

Query: 181 IKKIKREELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVI 240
           +KK+KREE RKKILLGFR+CGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRY MAVN+I
Sbjct: 181 MKKMKREESRKKILLGFRICGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNII 240

Query: 241 GFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNS 300
           GFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ                       
Sbjct: 241 GFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ----------------------- 300

Query: 301 HINYTVIIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIIS 360
                 IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMAT SLGLSIVAFVAFALSCIIS
Sbjct: 301 ------IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATASLGLSIVAFVAFALSCIIS 333

BLAST of Lsi02G015590 vs. NCBI nr
Match: XP_008458753.1 (PREDICTED: CASP-like protein 4A1 [Cucumis melo] >KAA0037507.1 CASP-like protein 4A1 [Cucumis melo var. makuwa] >TYK01982.1 CASP-like protein 4A1 [Cucumis melo var. makuwa])

HSP 1 Score: 483.0 bits (1242), Expect = 2.2e-132
Identity = 277/362 (76.52%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 2   AASNSEAEMKKKELAKTMEEEEEEERQQHHQNQSEE----NESHPPLSARSSPPHSLSSS 61
           +ASNSEAEMK++EL K+MEE +E+  +Q H NQS+E    NESH  LSA SSPPHSLS+S
Sbjct: 4   SASNSEAEMKREELPKSMEEPQEQVHEQPHHNQSDEGENDNESHLHLSACSSPPHSLSTS 63

Query: 62  KDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQP 121
           KDPSP LHSPSISSDFSDHTCH+ GNSSIDD      ++DLP  ANPPSPRPVAANRAQP
Sbjct: 64  KDPSPPLHSPSISSDFSDHTCHSRGNSSIDD------VQDLPPSANPPSPRPVAANRAQP 123

Query: 122 TEPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKR 181
           +EPIVVTKVD EIQGVRKVEEVS      D DGDVES D GKVGRGRKLM SLSIKKIK 
Sbjct: 124 SEPIVVTKVDTEIQGVRKVEEVS------DSDGDVESADGGKVGRGRKLMESLSIKKIKE 183

Query: 182 EELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSA 241
           EELRKKILLGFR+CGF+FCLVS SVMASDK+QGWALDSFYRYKEFRY MAVNVIGFVYSA
Sbjct: 184 EELRKKILLGFRICGFSFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSA 243

Query: 242 LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTV 301
           LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ                             
Sbjct: 244 LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQ----------------------------- 303

Query: 302 IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLCK 360
           IIAYLLLSASSSAATRIDDWQSNWG+DKFPDMAT SL LSIVAFVAFALSCIISGYTLCK
Sbjct: 304 IIAYLLLSASSSAATRIDDWQSNWGRDKFPDMATGSLALSIVAFVAFALSCIISGYTLCK 324

BLAST of Lsi02G015590 vs. NCBI nr
Match: XP_023550770.1 (CASP-like protein 4A1 [Cucurbita pepo subsp. pepo] >XP_023550771.1 CASP-like protein 4A1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481.5 bits (1238), Expect = 6.4e-132
Identity = 277/365 (75.89%), Postives = 296/365 (81.10%), Query Frame = 0

Query: 1   MAASNSEAEMKKKELAKTMEEEEEEERQQHHQNQSE-----ENESHPPLSARSSPPHSLS 60
           MAASNSEA MKK+ELA+TME+++   R Q   N++E     ++ESHPPLSA+SSPPHSLS
Sbjct: 1   MAASNSEAGMKKEELAETMEQQQLHRRNQSEVNENENENENDDESHPPLSAQSSPPHSLS 60

Query: 61  SSKDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRA 120
            +KDPSP LHSPS SSDFSDHTC THGNSSIDD LSITRLEDLP  A PPSPRPVAANRA
Sbjct: 61  DNKDPSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLEDLPPSAYPPSPRPVAANRA 120

Query: 121 QPTEPIVVTKVDAEIQGVRKVEEVSDGDGD-GDGDGDVESRDSGKVGRGRKLMASLSIKK 180
           QPTEPIVVTKVDAEIQGVRKVEE S  D +  DGDG       G VGRGRKLMA+LS+KK
Sbjct: 121 QPTEPIVVTKVDAEIQGVRKVEEGSASDVEPSDGDG-------GAVGRGRKLMANLSMKK 180

Query: 181 IKREELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFV 240
           IKREELRKKILLGFR+ GF FCL SFSVMASDKNQGWALDSFYRYKEFRY MAVNVIGFV
Sbjct: 181 IKREELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFV 240

Query: 241 YSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHIN 300
           YSALQSYDLVYFLSTGKHMIRN+FKQYFDFFIDQ                          
Sbjct: 241 YSALQSYDLVYFLSTGKHMIRNYFKQYFDFFIDQ-------------------------- 300

Query: 301 YTVIIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYT 360
              I+AYLLLSASSSAATR+DDWQSNWGKDKFPDMA TS+GLSIVAFVAFALSCIISGYT
Sbjct: 301 ---IVAYLLLSASSSAATRVDDWQSNWGKDKFPDMAITSVGLSIVAFVAFALSCIISGYT 329

BLAST of Lsi02G015590 vs. NCBI nr
Match: KAG6578643.1 (CASP-like protein 4A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 478.0 bits (1229), Expect = 7.1e-131
Identity = 274/361 (75.90%), Postives = 293/361 (81.16%), Query Frame = 0

Query: 1   MAASNSEAEMKKKELAKTMEEEEEEERQQHHQNQSE-ENESHPPLSARSSPPHSLSSSKD 60
           MAASNSEAE+KK+ELA+TME+++     Q   N +E ++ESHPPLSA+SSPPHSLS +KD
Sbjct: 1   MAASNSEAEVKKEELAETMEQQQLHRHSQSEVNDNENDDESHPPLSAQSSPPHSLSDNKD 60

Query: 61  PSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQPTE 120
           PSP LHSPS SSDFSDHTC THGNSSIDD LSITRLEDLP  A PPSPRPVAANRAQPTE
Sbjct: 61  PSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLEDLPPSAYPPSPRPVAANRAQPTE 120

Query: 121 PIVVTKVDAEIQGVRKVEEVSDGDGD-GDGDGDVESRDSGKVGRGRKLMASLSIKKIKRE 180
           PIVVTKVDAEIQGVRKVEE S  D +  DGDG       G VGRGRKLMA+LS+KKIKRE
Sbjct: 121 PIVVTKVDAEIQGVRKVEEGSASDVEPSDGDG-------GPVGRGRKLMANLSMKKIKRE 180

Query: 181 ELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSAL 240
           ELRKKILLGFR+ GF FCL SFSVMASDKNQGWALDSFYRYKEFRY MAVNVIGFVYSAL
Sbjct: 181 ELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSAL 240

Query: 241 QSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTVI 300
           QSYDL+YFLSTGKHMI N+FKQYFDFFIDQA                             
Sbjct: 241 QSYDLIYFLSTGKHMIHNYFKQYFDFFIDQA----------------------------- 300

Query: 301 IAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLCKS 360
           IAYLLLSASSSAATR+DDWQSNWG DKFPDMA TS+GLSIVAFVAFALSCIISGYTLCKS
Sbjct: 301 IAYLLLSASSSAATRVDDWQSNWGMDKFPDMAVTSVGLSIVAFVAFALSCIISGYTLCKS 325

BLAST of Lsi02G015590 vs. NCBI nr
Match: XP_004142358.1 (CASP-like protein 4A3 [Cucumis sativus] >KGN52378.1 hypothetical protein Csa_008911 [Cucumis sativus])

HSP 1 Score: 477.6 bits (1228), Expect = 9.2e-131
Identity = 275/362 (75.97%), Postives = 295/362 (81.49%), Query Frame = 0

Query: 2   AASNSEAEMKKKELAKTMEEEEEEERQQHHQNQ--SEENESHPPLSARSSPPHSLSSSKD 61
           +ASNSEAEMKK+EL K+MEE  E+E   H+Q+    ++NESH  LSA SSPPHSLS++KD
Sbjct: 6   SASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDNESHLHLSACSSPPHSLSTNKD 65

Query: 62  PSPLLHSPSISSDFSDHTCHTHGNSSIDDDLSITRLED--LPQPANPPSPRPVAANRAQP 121
           PSP LHSPSISSDFSDHTCH+ GNSSID  LSIT L+D  LP  AN PSPRPVAANRAQP
Sbjct: 66  PSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAANRAQP 125

Query: 122 TEPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKR 181
           +EPIVVTKVD EIQG+RKVEEV D DGD +  GD +  D GKVGRGRKLM +LSIKKIKR
Sbjct: 126 SEPIVVTKVDTEIQGIRKVEEVIDSDGDVE-SGDGDDGDGGKVGRGRKLMETLSIKKIKR 185

Query: 182 EELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSA 241
           EELRKKILLGFR+CGFAFCLVS SVMASDK+QGWALDSFYRYKEFRY MAVNVIGFVYSA
Sbjct: 186 EELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSA 245

Query: 242 LQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTV 301
           LQSYDLVYFLSTGKH+IRNHFKQYFDFFIDQ                             
Sbjct: 246 LQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQ----------------------------- 305

Query: 302 IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLCK 360
           IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMAT SLG SIVAFVAFALSCIISGYTLCK
Sbjct: 306 IIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATGSLGFSIVAFVAFALSCIISGYTLCK 337

BLAST of Lsi02G015590 vs. TAIR 10
Match: AT2G36330.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 161.0 bits (406), Expect = 1.8e-39
Identity = 118/321 (36.76%), Postives = 168/321 (52.34%), Query Frame = 0

Query: 42  PPLSARSSPPHSLSSSKDPSPLLHSPSISSDFSDHTCHTHGNSSIDDDLS-ITRLEDLPQ 101
           P  +  S  P S+S+ K P P   S +I + F + T H   + S    L   +  E    
Sbjct: 4   PAKTMPSMSPSSVSTEKSPPPSDTSMAIVA-FDNSTTHFSSSPSPPHSLDHSSESEKEDA 63

Query: 102 PANPPSPRPVAANRAQPT-EPIVVTKVDAEIQGVRKVEEVSDGDGDGDGDGDVESRDSGK 161
            + P S R     + + T  PIVV          R V+EV            V +R S +
Sbjct: 64  KSKPESRRNKNPGKVEETPSPIVVVH-----NHNRSVKEV------------VPTRKSAR 123

Query: 162 VGRGRKL-----MASLSIKKIKREELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALD 221
           VG GR         S  +++ +REE+ K   LGFR+      L+SFS+MA+DK +GW+ D
Sbjct: 124 VGSGRSSGQRSGAVSAILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGD 183

Query: 222 SFYRYKEFRYSMAVNVIGFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESAS 281
           SF RYKE+R+ ++VNV+ FVYS+ Q+ DL Y L   KH+I +H +  F+F IDQ      
Sbjct: 184 SFDRYKEYRFCLSVNVVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQ------ 243

Query: 282 SSHEELTNTFTLNPLNSHINYTVIIAYLLLSASSSAATRIDDWQSNWGKDKFPDMATTSL 341
                                  ++AYLL+SAS++A TR+DDW SNWGKD+F +MA+ S+
Sbjct: 244 -----------------------VLAYLLMSASTAAVTRVDDWVSNWGKDEFTEMASASI 277

Query: 342 GLSIVAFVAFALSCIISGYTL 356
            +S +AF+AFA S +ISGY L
Sbjct: 304 AMSFLAFLAFAFSSLISGYNL 277

BLAST of Lsi02G015590 vs. TAIR 10
Match: AT5G40300.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 147.9 bits (372), Expect = 1.6e-35
Identity = 124/344 (36.05%), Postives = 176/344 (51.16%), Query Frame = 0

Query: 13  KELAKTMEEEEEEERQQHHQNQSEENESHPPLSARSSPPHSLSSSKDPSPLLHSPSISSD 72
           +EL KT + ++++++QQ  Q+QS  +  +  +S+RSS  HSL  +   SP   SP++SS 
Sbjct: 2   EELEKTQKFQKKKKQQQEKQDQS--SPINFEMSSRSS-LHSLPQTTIESP-PDSPTLSS- 61

Query: 73  FSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQPTEPIVVTKVDAEIQG 132
                  +HG+                      SP  +      PT  +  T+    +  
Sbjct: 62  ----IPDSHGS----------------------SPHTII-----PTPSVAKTETPFRVTN 121

Query: 133 VRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKREELRKKILLGFRVCG 192
             + ++VS               +S +  R     +S + ++ K   L +K LLGFRV  
Sbjct: 122 GEEEKKVS---------------ESRRQLRPSFSSSSSTPRESKWASLIRKALLGFRVIA 181

Query: 193 FAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSALQSYDLVYFLSTGKH 252
           F  CLVSFSVM SD+++GWA DSFY YKEFR+ +A NVIGFVYS     DLVY LST   
Sbjct: 182 FVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVYSGFMICDLVYLLSTSIR 241

Query: 253 MIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTVIIAYLLLSASSSAAT 312
             R++ + + +F +DQ                             ++AYLL SAS+SA+ 
Sbjct: 242 RSRHNLRHFLEFGLDQ-----------------------------MLAYLLASASTSASI 265

Query: 313 RIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTLC 357
           R+DDWQSNWG DKFPD+A  S+ LS V+FVAFA   + SGY LC
Sbjct: 302 RVDDWQSNWGADKFPDLARASVALSYVSFVAFAFCSLASGYALC 265

BLAST of Lsi02G015590 vs. TAIR 10
Match: AT5G62820.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 131.0 bits (328), Expect = 2.0e-30
Identity = 112/347 (32.28%), Postives = 162/347 (46.69%), Query Frame = 0

Query: 10  MKKKELAKTMEEEEEEERQQHHQNQSEENESHPPLSARSSPPHSLSSSKDPSPL-LHSPS 69
           MK K  A +       E Q ++++       H PLS     P S   S + SP  + +P 
Sbjct: 1   MKMKRTASS-----NSEAQSYNESPHSPLRFHSPLSDAGDLPESRYVSPEGSPFKIENP- 60

Query: 70  ISSDFSDHTCHTHGNSSIDDDLSITRLEDLPQPANPPSPRPVAANRAQPTEPIVVTKVDA 129
                           SI     +T+   LP P  PP P+     R +     + +  D 
Sbjct: 61  ---------------KSIVAGNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSSSDK 120

Query: 130 EIQGVRKVEEVSDGDGDGDGDGDVESRDSGKVGRGRKLMASLSIKKIKREELRKKILLGF 189
               +         DG     G   +R +G    G +     ++ + + ++L     LGF
Sbjct: 121 SPSSMVVHNSWVREDG-----GQTTTRKAGAPINGEE-STRTTVNRARGDDLVSLTALGF 180

Query: 190 RVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVYSALQSYDLVYFLS 249
           R+     C++SFS+MA+DK QGW+ DS+ RYKE+RY +AVNVI FVYSA ++ D   +++
Sbjct: 181 RITEVILCVISFSIMAADKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIA 240

Query: 250 TGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINYTVIIAYLLLSASS 309
              +MI   F   F F +DQ                             ++AYLL+SASS
Sbjct: 241 KESYMINCGFHDLFVFSMDQ-----------------------------LLAYLLMSASS 291

Query: 310 SAATRIDDWQSNWGKDKFPDMATTSLGLSIVAFVAFALSCIISGYTL 356
            AATR+DDW SNWGKD+F  MAT S+ +S +AF AFA+S +IS Y L
Sbjct: 301 CAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVSALISSYRL 291

BLAST of Lsi02G015590 vs. TAIR 10
Match: AT2G38480.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 58.2 bits (139), Expect = 1.6e-08
Identity = 55/181 (30.39%), Postives = 91/181 (50.28%), Query Frame = 0

Query: 176 KREELRKKILLGFRVCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYSMAVNVIGFVY 235
           KRE+L KK     R     F L++F +M S+K+ G+   +F  Y+E+RY +A+++I  +Y
Sbjct: 39  KREDLIKKASPITRGICLLFSLIAFLIMVSNKH-GYG-RNFNDYEEYRYVLAISIISTLY 98

Query: 236 SALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESASSSHEELTNTFTLNPLNSHINY 295
           +A Q++   +F  + + +         DF  DQ                           
Sbjct: 99  TAWQTF--AHF--SKREIFDRRTSILVDFSGDQ--------------------------- 158

Query: 296 TVIIAYLLLSASSSAATRIDDWQSNWGKDK-FPDMATTSLGLSIVAFVAFALSCIISGYT 355
             I+AYLL+SA+SSA    + ++   G+D  F D A +++ ++I AF+A ALS + SGY 
Sbjct: 159 --IVAYLLISAASSAIPLTNIFRE--GQDNIFTDSAASAISMAIFAFIALALSALFSGYK 182

BLAST of Lsi02G015590 vs. TAIR 10
Match: AT4G11655.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 48.1 bits (113), Expect = 1.7e-05
Identity = 42/146 (28.77%), Postives = 61/146 (41.78%), Query Frame = 0

Query: 215 SFYRYKEFRYSMAVNVIGFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQAKESAS 274
           SF  Y E  Y   V VIGFVY++LQ++  V  ++    +I      Y  F  DQ      
Sbjct: 92  SFASYPELLYCFGVAVIGFVYTSLQTFKGVCDITHRGVLISEPLSDYISFIFDQ------ 151

Query: 275 SSHEELTNTFTLNPLNSHINYTVIIAYLLLSASSSAATRIDDWQSNWGKDKFPDM---AT 334
                                  +I YLL+S+SS A      W  +  +D    +   + 
Sbjct: 152 -----------------------VICYLLVSSSSVAIA----WIQHINEDAIKTLRNNSI 204

Query: 335 TSLGLSIVAFVAFALSCIISGYTLCK 358
            S+ +S  AF+   LS ++SGY LCK
Sbjct: 212 VSVSMSFSAFLVLTLSGLLSGYKLCK 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84WP52.5e-3836.76CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1[more]
D7LIR26.2e-3735.33CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q9FNE82.2e-3436.05CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1[more]
D7MMW42.5e-3032.33CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q501G62.8e-2932.28CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7T7H81.1e-13276.52CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00... [more]
A0A1S3C8441.1e-13276.52CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103498066 PE=3 SV=1[more]
A0A0A0KS014.5e-13175.97CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G630810 PE=3 SV=1[more]
A0A6J1FJD74.9e-13074.93CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444795 PE=3 SV=1[more]
A0A6J1JTE43.5e-12873.97CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489567 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038890250.19.8e-14982.61CASP-like protein 4A1 [Benincasa hispida][more]
XP_008458753.12.2e-13276.52PREDICTED: CASP-like protein 4A1 [Cucumis melo] >KAA0037507.1 CASP-like protein ... [more]
XP_023550770.16.4e-13275.89CASP-like protein 4A1 [Cucurbita pepo subsp. pepo] >XP_023550771.1 CASP-like pro... [more]
KAG6578643.17.1e-13175.90CASP-like protein 4A1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_004142358.19.2e-13175.97CASP-like protein 4A3 [Cucumis sativus] >KGN52378.1 hypothetical protein Csa_008... [more]
Match NameE-valueIdentityDescription
AT2G36330.11.8e-3936.76Uncharacterised protein family (UPF0497) [more]
AT5G40300.11.6e-3536.05Uncharacterised protein family (UPF0497) [more]
AT5G62820.12.0e-3032.28Uncharacterised protein family (UPF0497) [more]
AT2G38480.11.6e-0830.39Uncharacterised protein family (UPF0497) [more]
AT4G11655.11.7e-0528.77Uncharacterised protein family (UPF0497) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1..39
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..160
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..37
NoneNo IPR availablePANTHERPTHR11615:SF279CASP-LIKE PROTEIN 4A1coord: 87..358
NoneNo IPR availablePANTHERPTHR11615NITRATE, FORMATE, IRON DEHYDROGENASEcoord: 87..358
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 182..269
e-value: 4.6E-18
score: 65.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G015590.1Lsi02G015590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0030117 membrane coat
cellular_component GO:0005886 plasma membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005198 structural molecule activity