Lsi02G015330 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G015330
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	metalloendoproteinase 2-MMP-like
Location	chr02: 21199942 .. 21200826 (-)
RNA-Seq Expression	Lsi02G015330
Synteny	Lsi02G015330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTTAAGTTCTTTCTTCAACTCTTCCTCCTCTTTGCCTCCGTCGCACCTCACATGATCACGGCGCGTGTTCTTCACACCAACATATCCTCCCATCTTCAAGGAAGTCGTATAGGTAACAATACCGAAGGAATCCACAACGTCAAGCTTTACCTCCAACGTTATGGTTACTTAACCAACGTAAAGAGTACCAATCCTAATGTATTCGACGATCTCCTAGAATCAGCCATTAAAACATTCCAAAAATACCATAGCCTCAACGTGAGTGGCATTTTGGATAAGGAGACACTAGCTCTAATGTCTCAGCCTCGATGTGGAATTCCAGATATCTTGCACAATAATAATATCACTCATAGTGGCATTCAAATAAATAGTACCACCTTTCATTCTCATTTCTCATTCTTTCCAGGAAGTCCAAAATGGCCGATATCGAAATACCATTTGACGTACAGGTTTCTCGATAGTTTTCCAAATAACTTCGTAGCACCGGTGACGAACGCAATGGAGCAATGGGGGAAGTTCAGCAAGTTCACATTCTCAGTAGCCGCAAGGTCTCAAAAGGCAGACATCACATTTAACTTTGTGAGAGGAAACCATGGGGATGGCTATCCATTTGAGGGAAAAGGAGGGACTTTGGCGCATGCTTTTGGGCCACCAGATGGGAGGGTACACTTTGATGCGGACGAAAGTTGGGTGGATGGGTCTGTTAGTGGTTTCTTCAATGTGGGGATGGTGGCGTTGCATGAGCTTGGGCATGTGCTTGGGCTTGGCCACAGCACCATTCGAGACGCCATTATGTGGCCCTACATGAACCCCGGTGATCAGTTCAGGGGCTTACAGTTTGATGATATTCAAGGCATCCAAACTTTGTATAGTTCTTGA

mRNA sequence

ATGGCTCTTAAGTTCTTTCTTCAACTCTTCCTCCTCTTTGCCTCCGTCGCACCTCACATGATCACGGCGCGTGTTCTTCACACCAACATATCCTCCCATCTTCAAGGAAGTCGTATAGGTAACAATACCGAAGGAATCCACAACGTCAAGCTTTACCTCCAACGTTATGGTTACTTAACCAACGTAAAGAGTACCAATCCTAATGTATTCGACGATCTCCTAGAATCAGCCATTAAAACATTCCAAAAATACCATAGCCTCAACGTGAGTGGCATTTTGGATAAGGAGACACTAGCTCTAATGTCTCAGCCTCGATGTGGAATTCCAGATATCTTGCACAATAATAATATCACTCATAGTGGCATTCAAATAAATAGTACCACCTTTCATTCTCATTTCTCATTCTTTCCAGGAAGTCCAAAATGGCCGATATCGAAATACCATTTGACGTACAGGTTTCTCGATAGTTTTCCAAATAACTTCGTAGCACCGGTGACGAACGCAATGGAGCAATGGGGGAAGTTCAGCAAGTTCACATTCTCAGTAGCCGCAAGGTCTCAAAAGGCAGACATCACATTTAACTTTGTGAGAGGAAACCATGGGGATGGCTATCCATTTGAGGGAAAAGGAGGGACTTTGGCGCATGCTTTTGGGCCACCAGATGGGAGGGTACACTTTGATGCGGACGAAAGTTGGGTGGATGGGTCTGTTAGTGGTTTCTTCAATGTGGGGATGGTGGCGTTGCATGAGCTTGGGCATGTGCTTGGGCTTGGCCACAGCACCATTCGAGACGCCATTATGTGGCCCTACATGAACCCCGGTGATCAGTTCAGGGGCTTACAGTTTGATGATATTCAAGGCATCCAAACTTTGTATAGTTCTTGA

Coding sequence (CDS)

ATGGCTCTTAAGTTCTTTCTTCAACTCTTCCTCCTCTTTGCCTCCGTCGCACCTCACATGATCACGGCGCGTGTTCTTCACACCAACATATCCTCCCATCTTCAAGGAAGTCGTATAGGTAACAATACCGAAGGAATCCACAACGTCAAGCTTTACCTCCAACGTTATGGTTACTTAACCAACGTAAAGAGTACCAATCCTAATGTATTCGACGATCTCCTAGAATCAGCCATTAAAACATTCCAAAAATACCATAGCCTCAACGTGAGTGGCATTTTGGATAAGGAGACACTAGCTCTAATGTCTCAGCCTCGATGTGGAATTCCAGATATCTTGCACAATAATAATATCACTCATAGTGGCATTCAAATAAATAGTACCACCTTTCATTCTCATTTCTCATTCTTTCCAGGAAGTCCAAAATGGCCGATATCGAAATACCATTTGACGTACAGGTTTCTCGATAGTTTTCCAAATAACTTCGTAGCACCGGTGACGAACGCAATGGAGCAATGGGGGAAGTTCAGCAAGTTCACATTCTCAGTAGCCGCAAGGTCTCAAAAGGCAGACATCACATTTAACTTTGTGAGAGGAAACCATGGGGATGGCTATCCATTTGAGGGAAAAGGAGGGACTTTGGCGCATGCTTTTGGGCCACCAGATGGGAGGGTACACTTTGATGCGGACGAAAGTTGGGTGGATGGGTCTGTTAGTGGTTTCTTCAATGTGGGGATGGTGGCGTTGCATGAGCTTGGGCATGTGCTTGGGCTTGGCCACAGCACCATTCGAGACGCCATTATGTGGCCCTACATGAACCCCGGTGATCAGTTCAGGGGCTTACAGTTTGATGATATTCAAGGCATCCAAACTTTGTATAGTTCTTGA

Protein sequence

MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYSS

Homology

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BLAST of Lsi02G015330 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-52
Identity = 114/318 (35.85%), Postives = 178/318 (55.97%), Query Frame = 0


Query: 6   FLQLFLLFA-----------SVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQ 65
           FL LFL+ +           +V P +     +  +  S+  G   G N +G++ +K Y Q
Sbjct: 8   FLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQ 67

Query: 66  RYGYLTNVKSTN-PNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILH 125
           R+GY+    S N  + FDD+L++A++ +Q   +LNV+G LD  T+  +  PRCG PD+++
Sbjct: 68  RFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVN 127

Query: 126 NNNITHSG----IQINSTTFHSH----FSFFPGSPKWPISKYHLTYRFLDSFP--NNFVA 185
             ++ H G     ++N +  H H    ++ FPG P+WP ++  LTY F    P      +
Sbjct: 128 GTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKS 187

Query: 186 PVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGR 245
             + A  +W   +   F+++     +DIT  F  G+HGDG PF+G  GTLAHAF PP G+
Sbjct: 188 VFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGK 247

Query: 246 VHFDADESW-VDGSVSGFFNV------GMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQ 295
            H DADE+W V G +  F +V        VA+HE+GH+LGLGHS++ ++IM+P +  G +
Sbjct: 248 FHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKR 307

[Close]

BLAST of Lsi02G015330 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.9e-52
Identity = 112/274 (40.88%), Postives = 158/274 (57.66%), Query Frame = 0


Query: 32  SHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSG 91
           S L G  IG N  G+  +K Y +R+GY+T   +   + FDD+L+SAI T+QK  +L V+G
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDD-FDDVLQSAINTYQKNFNLKVTG 105

Query: 92  ILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTY 151
            LD  TL  + +PRCG PD++   +  + G  + +T     +SFFPG P+WP  K  LTY
Sbjct: 106 KLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTT---EKYSFFPGKPRWPKRKRDLTY 165

Query: 152 RFLDSFPNNFVAPV----TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFE 211
            F     NN    V    + A  +W + +   F+ +    +ADI   F  G HGDG PF+
Sbjct: 166 AFAPQ--NNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFD 225

Query: 212 GKGGTLAHAFGPPDGRVHFDADESWVDGS---------VSGFFNVGMVALHELGHVLGLG 271
           G  GTLAHA  PP G +H D DE W+  +         V+   ++  VA+HE+GH+LGLG
Sbjct: 226 GAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLG 285

Query: 272 HSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 293
           HS++ DAIM+P ++ GD+   L  DDI+GIQ LY
Sbjct: 286 HSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

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BLAST of Lsi02G015330 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 3.6e-51
Identity = 104/280 (37.14%), Postives = 162/280 (57.86%), Query Frame = 0


Query: 36  GSRIGNNTEGIHNVKLYLQRYGYL--TNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGIL 95
           G   G   +G++ +K Y Q +GY+  TN+     + FDD+L++A++ +Q+   LNV+G+L
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 96  DKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFH---------SHFSFFPGSPKWPI 155
           D+ TL  +  PRCG PD+++  +  HSG +    +F           H+SFFPG P+WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 156 SKYHLTYRF--LDSFPNNFVAPVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGD 215
           ++  LTY F   ++      +  + A  +W + +  TF+   R   +DI+  F  G HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 216 GYPFEGKGGTLAHAFGPPDGRVHFDADESWV------DG--SVSGFFNVGMVALHELGHV 275
           G PF+G   TLAHAF PP G  H D +E+W+      DG  SVS   ++  VA+HE+GH+
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 276 LGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYSS 295
           LGLGHS++  +IM+P +  G +   L  DD++G+Q LY +
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

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BLAST of Lsi02G015330 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 197.6 bits (501), Expect = 2.0e-49
Identity = 110/261 (42.15%), Postives = 142/261 (54.41%), Query Frame = 0


Query: 40  GNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLA 99
           G N +G+ NVK Y    GY+ N    + N FDD L SAIKT+QK ++LNV+G  D  TL 
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLK 107

Query: 100 LMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDS--F 159
            +  PRCG+PDI+ N N T S   I      S ++FF   P+W      LTY F      
Sbjct: 108 QIMTPRCGVPDIIINTNKTTSFGMI------SDYTFFKDMPRWQAGTTQLTYAFSPEPRL 167

Query: 160 PNNFVAPVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAF 219
            + F + +  A  +W       F      + A+I   F   NHGD YPF+G GG L HAF
Sbjct: 168 DDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAF 227

Query: 220 GPPDGRVHFDADESWV------DGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYM 279
            P DGR HFDADE WV         V+  F++  VA+HE+GH+LGLGHS+   AIM+P +
Sbjct: 228 APTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSI 287

Query: 280 NPGDQFRGLQFDDIQGIQTLY 293
            P  +   L  DDI GI+ LY
Sbjct: 288 PPRTRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of Lsi02G015330 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.3e-44
Identity = 105/272 (38.60%), Postives = 146/272 (53.68%), Query Frame = 0


Query: 32  SHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSG 91
           S L   +IG++  G+  +K YL R+GY+ +      +VFD  LESAI  +Q+   L ++G
Sbjct: 56  SRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITG 115

Query: 92  ILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTY 151
            LD  T+ LMS PRCG+ D       TH  I  +     +H+++F G PKW  ++  LTY
Sbjct: 116 RLDTSTVTLMSLPRCGVSD-------THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTY 175

Query: 152 RF-----LDSFPNNFVAPV-TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYP 211
                  LD   +  V  V   A  QW      +F        AD+   F  G+HGDG P
Sbjct: 176 AISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLP 235

Query: 212 FEGKGGTLAHAFGPPDGRVHFDADESW-VDGSVSGFFNVGM----VALHELGHVLGLGHS 271
           F+G  GTLAHAF P +GR+H DA E+W VD  + G   V +    VA HE+GH+LGLGHS
Sbjct: 236 FDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHS 295

Query: 272 TIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 293
           +   A+M+P + P  +   L  DD+ G+  LY
Sbjct: 296 SQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Lsi02G015330 vs. ExPASy TrEMBL
Match: A0A0A0KU02 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632080 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.4e-130
Identity = 227/294 (77.21%), Postives = 254/294 (86.39%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYLT 60
           MAL+F LQLFLL AS+APH+ITARV HT+ISSHLQGSRIGNN EGIHNVKLYL+RYGYLT
Sbjct: 1   MALQFSLQLFLLIASIAPHVITARVRHTHISSHLQGSRIGNNIEGIHNVKLYLKRYGYLT 60

Query: 61  NVKSTNP-NVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNITH 120
           NV+STN  N FD LLESAIKTFQKYHSLNVSG++D+ETL LMS PRCGIPDI+HN N+  
Sbjct: 61  NVESTNSNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDIMHNINVMD 120

Query: 121 SGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKFT 180
           + +Q+NS++FHSHF+FFP + KWP+SKY+L Y FLD FPN+F  PV NAMEQW  FS F 
Sbjct: 121 NNVQMNSSSFHSHFTFFPDNLKWPVSKYNLRYTFLDDFPNDFKEPVMNAMEQWALFSLFR 180

Query: 181 FSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVSG 240
           FS A  +Q+ADITFNFVRGNHGDGYPF+GKGG LAHAFGP DGRVHFD DE W DGSV G
Sbjct: 181 FSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVGG 240

Query: 241 FFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
           F NVGMVALHELGHVLGL HSTIRDAIMWPYM  G+Q RGLQFDDI+GIQTLY+
Sbjct: 241 FINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQTLYA 294

[Close]

BLAST of Lsi02G015330 vs. ExPASy TrEMBL
Match: A0A1S3C931 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498173 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 7.4e-124
Identity = 219/295 (74.24%), Postives = 250/295 (84.75%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYL- 60
           MAL+  LQLFLL ASVAPH+ITARV HT+I SHL+GSRIGNN EGI++VKLYL+RYGYL 
Sbjct: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60

Query: 61  TNVKSTNPN-VFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNIT 120
           TNV +T+ N  FD LLESAIKTFQKYHSLNVSG+LDKETL LMS PRCGI DI+HNNN+ 
Sbjct: 61  TNVPNTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHNNNVM 120

Query: 121 HSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKF 180
            + IQ+NS++FHSH++F PG+PKWPISKY+L Y FL+ FPN+F  PV NAM+QW  FS F
Sbjct: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180

Query: 181 TFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVS 240
            FS     ++ DITFNFVRGNHGDG+PF+GKGG LAHAFGP DGRVHFD DE W DGSV 
Sbjct: 181 RFSETIGGEEVDITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
           GF NVGMVALHELGHVLGL HSTIRDAIMWPYM+ G+Q RGLQFDDI+GIQTLY+
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMHVGEQTRGLQFDDIEGIQTLYA 295

[Close]

BLAST of Lsi02G015330 vs. ExPASy TrEMBL
Match: A0A5D3BRW3 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00130 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 9.6e-124
Identity = 219/295 (74.24%), Postives = 251/295 (85.08%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYL- 60
           MAL+  LQLFLL ASVAPH+ITARV HT+I SHL+GSRIGNN EGI++VKLYL+RYGYL 
Sbjct: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60

Query: 61  TNVKSTNPN-VFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNIT 120
           TNV +T+ N  FD LLESAIKTFQKYHSLNVSG+LDKETL LMS PRCGI DI+H+NN+ 
Sbjct: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120

Query: 121 HSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKF 180
            + IQ+NS++FHSH++F PG+PKWPISKY+L Y FL+ FPN+F  PV NAM+QW  FS F
Sbjct: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180

Query: 181 TFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVS 240
            FS     ++ADITFNFVRGNHGDG+PF+GKGG LAHAFGP DGRVHFD DE W DGSV 
Sbjct: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
           GF NVGMVALHELGHVLGL HSTIRDAIMWPYM+ G+Q RGLQFDDI+GIQTLY+
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYA 295

[Close]

BLAST of Lsi02G015330 vs. ExPASy TrEMBL
Match: A0A6J1BVK9 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006145 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 7.2e-87
Identity = 173/301 (57.48%), Postives = 210/301 (69.77%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVL--HTNISSH------LQGSRIGNNTEGIHNVKLY 60
           MA KF  QL L    +A H+I+AR L  H++ S+       L+GSR GNN EGIHNVK+Y
Sbjct: 1   MAFKFSPQLQLQLLLLAAHVISARELGSHSHKSAQFVFPQCLEGSRKGNNIEGIHNVKIY 60

Query: 61  LQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDIL 120
           L RYGYLTN   T+ NVFDD LESA+ T+Q+ + LNVSG+LD+ETLA+MS+PRCG+PDI+
Sbjct: 61  LHRYGYLTNDIETDYNVFDDALESALITYQRNYRLNVSGVLDRETLAVMSKPRCGLPDIV 120

Query: 121 HNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQW 180
            ++N        N     +HF+F PG PKWP SKYHLTY F+  FP  F   V  A EQW
Sbjct: 121 QSHN--------NKNISQAHFTFMPGLPKWPKSKYHLTYGFIKDFPPKFAMAVREAFEQW 180

Query: 181 GKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESW 240
              S FTFS A R Q ADIT +F RG+HGD YPF+G  G LAHAF P DGRVHFDADE+W
Sbjct: 181 EGNSVFTFSPATRVQSADITLSFERGDHGDPYPFDGPWGKLAHAFRPGDGRVHFDADENW 240

Query: 241 VDGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 294
           V G     F++ MVALHELGH LGLGHS I++A+MW Y++PG   +GL  DDI G+QTLY
Sbjct: 241 VVGPREDAFDLVMVALHELGHALGLGHSFIQEAVMWAYLDPGVS-KGLNMDDIVGLQTLY 292

[Close]

BLAST of Lsi02G015330 vs. ExPASy TrEMBL
Match: A0A6J1BZK9 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006143 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 3.7e-83
Identity = 160/298 (53.69%), Postives = 205/298 (68.79%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYL- 60
           MA K      LL ASVAP +I +   H     HLQG R G+N EGI NVK++LQRYGY+ 
Sbjct: 1   MAFKCLKLFLLLLASVAPRVILSHDHHIQFPEHLQGCRKGDNVEGIQNVKMHLQRYGYMR 60

Query: 61  ----TNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNN 120
                 V   + N FD+ LESAIK +QK  +LN+SG LD+ETL LMS+PRCG+PDI+ ++
Sbjct: 61  KDNNNTVNKESTNAFDEALESAIKKYQKNFNLNLSGALDRETLDLMSRPRCGVPDIVASS 120

Query: 121 NITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKF 180
           N  +  +  NS    SH++FFPG+ KWP +KYHLTY F++++P+N+VA V  A+  W   
Sbjct: 121 N--NETMMWNS----SHYAFFPGNLKWPNTKYHLTYTFINNYPSNYVAAVARALATWAGA 180

Query: 181 SKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDG 240
           S+FTF+ A   + ADI  +F RGNHGDG PF+G+GG LAHAF P DGR+H DADESW +G
Sbjct: 181 SQFTFAEAFEGETADIKISFERGNHGDGNPFDGRGGVLAHAFAPSDGRLHLDADESWTEG 240

Query: 241 SVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
            V   F+V  VALHELGH+LGLGHS I  AIMWP+++PG   +GL  DDI GI  LY+
Sbjct: 241 VVFNEFDVETVALHELGHILGLGHSKIEAAIMWPFLDPG-ATKGLNADDIDGIHALYA 291

[Close]

BLAST of Lsi02G015330 vs. NCBI nr
Match: XP_004142465.1 (metalloendoproteinase 5-MMP [Cucumis sativus] >KGN52409.1 hypothetical protein Csa_009097 [Cucumis sativus])

HSP 1 Score: 475.7 bits (1223), Expect = 2.9e-130
Identity = 227/294 (77.21%), Postives = 254/294 (86.39%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYLT 60
           MAL+F LQLFLL AS+APH+ITARV HT+ISSHLQGSRIGNN EGIHNVKLYL+RYGYLT
Sbjct: 1   MALQFSLQLFLLIASIAPHVITARVRHTHISSHLQGSRIGNNIEGIHNVKLYLKRYGYLT 60

Query: 61  NVKSTNP-NVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNITH 120
           NV+STN  N FD LLESAIKTFQKYHSLNVSG++D+ETL LMS PRCGIPDI+HN N+  
Sbjct: 61  NVESTNSNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDIMHNINVMD 120

Query: 121 SGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKFT 180
           + +Q+NS++FHSHF+FFP + KWP+SKY+L Y FLD FPN+F  PV NAMEQW  FS F 
Sbjct: 121 NNVQMNSSSFHSHFTFFPDNLKWPVSKYNLRYTFLDDFPNDFKEPVMNAMEQWALFSLFR 180

Query: 181 FSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVSG 240
           FS A  +Q+ADITFNFVRGNHGDGYPF+GKGG LAHAFGP DGRVHFD DE W DGSV G
Sbjct: 181 FSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVGG 240

Query: 241 FFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
           F NVGMVALHELGHVLGL HSTIRDAIMWPYM  G+Q RGLQFDDI+GIQTLY+
Sbjct: 241 FINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQTLYA 294

[Close]

BLAST of Lsi02G015330 vs. NCBI nr
Match: XP_008458910.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo])

HSP 1 Score: 453.4 bits (1165), Expect = 1.5e-123
Identity = 219/295 (74.24%), Postives = 250/295 (84.75%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYL- 60
           MAL+  LQLFLL ASVAPH+ITARV HT+I SHL+GSRIGNN EGI++VKLYL+RYGYL 
Sbjct: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60

Query: 61  TNVKSTNPN-VFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNIT 120
           TNV +T+ N  FD LLESAIKTFQKYHSLNVSG+LDKETL LMS PRCGI DI+HNNN+ 
Sbjct: 61  TNVPNTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHNNNVM 120

Query: 121 HSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKF 180
            + IQ+NS++FHSH++F PG+PKWPISKY+L Y FL+ FPN+F  PV NAM+QW  FS F
Sbjct: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180

Query: 181 TFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVS 240
            FS     ++ DITFNFVRGNHGDG+PF+GKGG LAHAFGP DGRVHFD DE W DGSV 
Sbjct: 181 RFSETIGGEEVDITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
           GF NVGMVALHELGHVLGL HSTIRDAIMWPYM+ G+Q RGLQFDDI+GIQTLY+
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMHVGEQTRGLQFDDIEGIQTLYA 295

[Close]

BLAST of Lsi02G015330 vs. NCBI nr
Match: KAA0037451.1 (metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK01925.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 453.0 bits (1164), Expect = 2.0e-123
Identity = 219/295 (74.24%), Postives = 251/295 (85.08%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYL- 60
           MAL+  LQLFLL ASVAPH+ITARV HT+I SHL+GSRIGNN EGI++VKLYL+RYGYL 
Sbjct: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60

Query: 61  TNVKSTNPN-VFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNNNIT 120
           TNV +T+ N  FD LLESAIKTFQKYHSLNVSG+LDKETL LMS PRCGI DI+H+NN+ 
Sbjct: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120

Query: 121 HSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKFSKF 180
            + IQ+NS++FHSH++F PG+PKWPISKY+L Y FL+ FPN+F  PV NAM+QW  FS F
Sbjct: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180

Query: 181 TFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDGSVS 240
            FS     ++ADITFNFVRGNHGDG+PF+GKGG LAHAFGP DGRVHFD DE W DGSV 
Sbjct: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
           GF NVGMVALHELGHVLGL HSTIRDAIMWPYM+ G+Q RGLQFDDI+GIQTLY+
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYA 295

[Close]

BLAST of Lsi02G015330 vs. NCBI nr
Match: XP_022133606.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 330.5 bits (846), Expect = 1.5e-86
Identity = 173/301 (57.48%), Postives = 210/301 (69.77%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVL--HTNISSH------LQGSRIGNNTEGIHNVKLY 60
           MA KF  QL L    +A H+I+AR L  H++ S+       L+GSR GNN EGIHNVK+Y
Sbjct: 1   MAFKFSPQLQLQLLLLAAHVISARELGSHSHKSAQFVFPQCLEGSRKGNNIEGIHNVKIY 60

Query: 61  LQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDIL 120
           L RYGYLTN   T+ NVFDD LESA+ T+Q+ + LNVSG+LD+ETLA+MS+PRCG+PDI+
Sbjct: 61  LHRYGYLTNDIETDYNVFDDALESALITYQRNYRLNVSGVLDRETLAVMSKPRCGLPDIV 120

Query: 121 HNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQW 180
            ++N        N     +HF+F PG PKWP SKYHLTY F+  FP  F   V  A EQW
Sbjct: 121 QSHN--------NKNISQAHFTFMPGLPKWPKSKYHLTYGFIKDFPPKFAMAVREAFEQW 180

Query: 181 GKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESW 240
              S FTFS A R Q ADIT +F RG+HGD YPF+G  G LAHAF P DGRVHFDADE+W
Sbjct: 181 EGNSVFTFSPATRVQSADITLSFERGDHGDPYPFDGPWGKLAHAFRPGDGRVHFDADENW 240

Query: 241 VDGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 294
           V G     F++ MVALHELGH LGLGHS I++A+MW Y++PG   +GL  DDI G+QTLY
Sbjct: 241 VVGPREDAFDLVMVALHELGHALGLGHSFIQEAVMWAYLDPGVS-KGLNMDDIVGLQTLY 292

[Close]

BLAST of Lsi02G015330 vs. NCBI nr
Match: XP_022133603.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 318.2 bits (814), Expect = 7.7e-83
Identity = 160/298 (53.69%), Postives = 205/298 (68.79%), Query Frame = 0


Query: 1   MALKFFLQLFLLFASVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQRYGYL- 60
           MA K      LL ASVAP +I +   H     HLQG R G+N EGI NVK++LQRYGY+ 
Sbjct: 1   MAFKCLKLFLLLLASVAPRVILSHDHHIQFPEHLQGCRKGDNVEGIQNVKMHLQRYGYMR 60

Query: 61  ----TNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILHNN 120
                 V   + N FD+ LESAIK +QK  +LN+SG LD+ETL LMS+PRCG+PDI+ ++
Sbjct: 61  KDNNNTVNKESTNAFDEALESAIKKYQKNFNLNLSGALDRETLDLMSRPRCGVPDIVASS 120

Query: 121 NITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTYRFLDSFPNNFVAPVTNAMEQWGKF 180
           N  +  +  NS    SH++FFPG+ KWP +KYHLTY F++++P+N+VA V  A+  W   
Sbjct: 121 N--NETMMWNS----SHYAFFPGNLKWPNTKYHLTYTFINNYPSNYVAAVARALATWAGA 180

Query: 181 SKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGRVHFDADESWVDG 240
           S+FTF+ A   + ADI  +F RGNHGDG PF+G+GG LAHAF P DGR+H DADESW +G
Sbjct: 181 SQFTFAEAFEGETADIKISFERGNHGDGNPFDGRGGVLAHAFAPSDGRLHLDADESWTEG 240

Query: 241 SVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYS 294
            V   F+V  VALHELGH+LGLGHS I  AIMWP+++PG   +GL  DDI GI  LY+
Sbjct: 241 VVFNEFDVETVALHELGHILGLGHSKIEAAIMWPFLDPG-ATKGLNADDIDGIHALYA 291

[Close]

BLAST of Lsi02G015330 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 208.4 bits (529), Expect = 8.0e-54
Identity = 114/318 (35.85%), Postives = 178/318 (55.97%), Query Frame = 0


Query: 6   FLQLFLLFA-----------SVAPHMITARVLHTNISSHLQGSRIGNNTEGIHNVKLYLQ 65
           FL LFL+ +           +V P +     +  +  S+  G   G N +G++ +K Y Q
Sbjct: 8   FLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQ 67

Query: 66  RYGYLTNVKSTN-PNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPRCGIPDILH 125
           R+GY+    S N  + FDD+L++A++ +Q   +LNV+G LD  T+  +  PRCG PD+++
Sbjct: 68  RFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVN 127

Query: 126 NNNITHSG----IQINSTTFHSH----FSFFPGSPKWPISKYHLTYRFLDSFP--NNFVA 185
             ++ H G     ++N +  H H    ++ FPG P+WP ++  LTY F    P      +
Sbjct: 128 GTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKS 187

Query: 186 PVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHAFGPPDGR 245
             + A  +W   +   F+++     +DIT  F  G+HGDG PF+G  GTLAHAF PP G+
Sbjct: 188 VFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGK 247

Query: 246 VHFDADESW-VDGSVSGFFNV------GMVALHELGHVLGLGHSTIRDAIMWPYMNPGDQ 295
            H DADE+W V G +  F +V        VA+HE+GH+LGLGHS++ ++IM+P +  G +
Sbjct: 248 FHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKR 307

[Close]

BLAST of Lsi02G015330 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-53
Identity = 112/274 (40.88%), Postives = 158/274 (57.66%), Query Frame = 0


Query: 32  SHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSG 91
           S L G  IG N  G+  +K Y +R+GY+T   +   + FDD+L+SAI T+QK  +L V+G
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDD-FDDVLQSAINTYQKNFNLKVTG 105

Query: 92  ILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTY 151
            LD  TL  + +PRCG PD++   +  + G  + +T     +SFFPG P+WP  K  LTY
Sbjct: 106 KLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTT---EKYSFFPGKPRWPKRKRDLTY 165

Query: 152 RFLDSFPNNFVAPV----TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFE 211
            F     NN    V    + A  +W + +   F+ +    +ADI   F  G HGDG PF+
Sbjct: 166 AFAPQ--NNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFD 225

Query: 212 GKGGTLAHAFGPPDGRVHFDADESWVDGS---------VSGFFNVGMVALHELGHVLGLG 271
           G  GTLAHA  PP G +H D DE W+  +         V+   ++  VA+HE+GH+LGLG
Sbjct: 226 GAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLG 285

Query: 272 HSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 293
           HS++ DAIM+P ++ GD+   L  DDI+GIQ LY
Sbjct: 286 HSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of Lsi02G015330 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 203.4 bits (516), Expect = 2.6e-52
Identity = 104/280 (37.14%), Postives = 162/280 (57.86%), Query Frame = 0


Query: 36  GSRIGNNTEGIHNVKLYLQRYGYL--TNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGIL 95
           G   G   +G++ +K Y Q +GY+  TN+     + FDD+L++A++ +Q+   LNV+G+L
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 96  DKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFH---------SHFSFFPGSPKWPI 155
           D+ TL  +  PRCG PD+++  +  HSG +    +F           H+SFFPG P+WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 156 SKYHLTYRF--LDSFPNNFVAPVTNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGD 215
           ++  LTY F   ++      +  + A  +W + +  TF+   R   +DI+  F  G HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 216 GYPFEGKGGTLAHAFGPPDGRVHFDADESWV------DG--SVSGFFNVGMVALHELGHV 275
           G PF+G   TLAHAF PP G  H D +E+W+      DG  SVS   ++  VA+HE+GH+
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 276 LGLGHSTIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLYSS 295
           LGLGHS++  +IM+P +  G +   L  DD++G+Q LY +
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

[Close]

BLAST of Lsi02G015330 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 179.9 bits (455), Expect = 3.0e-45
Identity = 105/272 (38.60%), Postives = 146/272 (53.68%), Query Frame = 0


Query: 32  SHLQGSRIGNNTEGIHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSG 91
           S L   +IG++  G+  +K YL R+GY+ +      +VFD  LESAI  +Q+   L ++G
Sbjct: 56  SRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSEIFSDVFDGPLESAISLYQENLGLPITG 115

Query: 92  ILDKETLALMSQPRCGIPDILHNNNITHSGIQINSTTFHSHFSFFPGSPKWPISKYHLTY 151
            LD  T+ LMS PRCG+ D       TH  I  +     +H+++F G PKW  ++  LTY
Sbjct: 116 RLDTSTVTLMSLPRCGVSD-------THMTINNDFLHTTAHYTYFNGKPKW--NRDTLTY 175

Query: 152 RF-----LDSFPNNFVAPV-TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYP 211
                  LD   +  V  V   A  QW      +F        AD+   F  G+HGDG P
Sbjct: 176 AISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLP 235

Query: 212 FEGKGGTLAHAFGPPDGRVHFDADESW-VDGSVSGFFNVGM----VALHELGHVLGLGHS 271
           F+G  GTLAHAF P +GR+H DA E+W VD  + G   V +    VA HE+GH+LGLGHS
Sbjct: 236 FDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHS 295

Query: 272 TIRDAIMWPYMNPGDQFRGLQFDDIQGIQTLY 293
           +   A+M+P + P  +   L  DD+ G+  LY
Sbjct: 296 SQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Lsi02G015330 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 157.1 bits (396), Expect = 2.1e-38
Identity = 91/262 (34.73%), Postives = 134/262 (51.15%), Query Frame = 0


Query: 46  IHNVKLYLQRYGYLTNVKSTNPNVFDDLLESAIKTFQKYHSLNVSGILDKETLALMSQPR 105
           I  +K +LQ+YGYL   K ++    D   E A+  +QK   L ++G  D +TL+ +  PR
Sbjct: 50  IPEIKRHLQQYGYLPQNKESD----DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPR 109

Query: 106 CGIPDILHNNNITHSGIQINSTTFHS--HFSFFPGSPKWPIS-KYHLTYRFLDSFPNNFV 165
           CG PD           ++  +  FH+   + +FPG P+W       LTY F       ++
Sbjct: 110 CGFPD----------DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYL 169

Query: 166 APV------TNAMEQWGKFSKFTFSVAARSQKADITFNFVRGNHGDGYPFEGKGGTLAHA 225
           AP         A  +W      +F        ADI   F  G+HGDG PF+G  G LAH 
Sbjct: 170 APTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHT 229

Query: 226 FGPPDGRVHFDADESWV----DGSVSGFFNVGMVALHELGHVLGLGHSTIRDAIMWPYMN 285
           F P +GR+H D  E+W     +   S   ++  VA+HE+GHVLGLGHS+++DA M+P + 
Sbjct: 230 FSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLK 289

Query: 286 PGDQFRGLQFDDIQGIQTLYSS 295
           P  +   L  DD+ G+Q+LY +
Sbjct: 290 PRSKKVNLNMDDVVGVQSLYGT 297

The following BLAST results are available for this feature:

BLAST of Lsi02G015330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04529	1.1e-52	35.85	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q9ZUJ5	1.9e-52	40.88	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
Q5XF51	3.6e-51	37.14	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
P29136	2.0e-49	42.15	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
O23507	4.3e-44	38.60	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of Lsi02G015330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KU02	1.4e-130	77.21	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632080 PE=3 S...	[more]
A0A1S3C931	7.4e-124	74.24	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498173 PE=3 SV...	[more]
A0A5D3BRW3	9.6e-124	74.24	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1BVK9	7.2e-87	57.48	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006145 PE=3...	[more]
A0A6J1BZK9	3.7e-83	53.69	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006143 PE=3...	[more]

BLAST of Lsi02G015330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142465.1	2.9e-130	77.21	metalloendoproteinase 5-MMP [Cucumis sativus] >KGN52409.1 hypothetical protein C...	[more]
XP_008458910.1	1.5e-123	74.24	PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo]	[more]
KAA0037451.1	2.0e-123	74.24	metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK01925.1 metalloe...	[more]
XP_022133606.1	1.5e-86	57.48	metalloendoproteinase 1-like [Momordica charantia]	[more]
XP_022133603.1	7.7e-83	53.69	metalloendoproteinase 1-like [Momordica charantia]	[more]

BLAST of Lsi02G015330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70170.1	8.0e-54	35.85	matrix metalloproteinase	[more]
AT1G59970.1	1.4e-53	40.88	Matrixin family protein	[more]
AT1G24140.1	2.6e-52	37.14	Matrixin family protein	[more]
AT4G16640.1	3.0e-45	38.60	Matrixin family protein	[more]
AT2G45040.1	2.1e-38	34.73	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 246..271 score: 56.52 coord: 101..114 score: 49.22 coord: 165..180 score: 23.55 coord: 280..293 score: 57.75 coord: 189..217 score: 58.96
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 138..294 e-value: 3.2E-31 score: 119.7
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 35..294 e-value: 4.8E-74 score: 250.9
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 44..101 e-value: 3.8E-13 score: 49.4
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 146..292 e-value: 9.7E-40 score: 136.0
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 23..293
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 23..293
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 135..292
IPR021158	Peptidase M10A, cysteine switch, zinc binding site	PROSITE	PS00546	CYSTEINE_SWITCH	coord: 104..111
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 141..292 e-value: 2.2157E-55 score: 174.7
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 38..111

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G015330.1	Lsi02G015330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity