Lsi02G015300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G015300
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	metalloendoproteinase 2-MMP-like
Location	chr02: 21181114 .. 21182160 (-)
RNA-Seq Expression	Lsi02G015300
Synteny	Lsi02G015300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTTCCAAAGCTCTCCAAATGATCTTCCCTTTCATAGGCCTTATATTTCTTTCCTTCTTCCCGTATCCAAATATATCGAATCCACTGATTTTGAAACACAATTCCCACGATATAAACTCATCTTCCTTAATATTTCTAAAGAAGAATCTCCAAGGATGTCGTTTGGGAGATACAATACAAGGAATTCATCAAATAAAAAAATACCTTCAACGTTTTGGTTACATTACCAATATCCAAAAGAACTCCAACCCAATATTTGATGACACTTTTGACCAAGTCTTGGAGTCCTCCCTTAAAACTTACCAAAAAAACCACAATCTTTTTGCCTCTGGAATCTTGGATTCCAACACCATTGCCCAAATGGCTATGCCTCGATGTGGGGTTGAAGATGTAATGAATGGAACAACTTGGACGAAATCAAGAAGAAGAAAAAGAAACCAAAATTACAGAAATGGCCATACGCATTTCCACAAAGTTTCTCACTTTGCTTTCTTTGAAGACAACCCAAAATGGCCAGCTTCAAAGTTTCACTTGAGCTATGCATTTCTTCGAGGCTACCCAACTGAAGCAATAATGGCAGTGTCAAGAGCTTTTTCAAAGTGGGCTTTCAATACCTATTTCACATTTTCACATGTTGTAGACTATAAGAAAGCTGATATCAAAATAAGCTTTGAGAGAGAAGAGCATGGAGATATTGATCCGTTTGATGGTACGGGAAAAGTTTTGGCCCATGCATTTCCACCAACGGATGGGAGACTTCATTTTGATGCTGATGAGGCTTGGTCTGTGGGAGCAATTTCAGGTTATTTTGATGTGGAAACTGTGGCTTTGCATGAGATTGGACATCTTCTTGGGCTTCAACATAGCTCCGTTCAAGGGGCTATCATGTTTCCAACTATACCTGAGGGAGAAACCAAGGGTTTGCATGGTGATGATATTGCTGGAATTAAGGCCTTATATGAATGGCTTCAAATTAATTTGATCTCTAAAATGTTTGTTGGCTTTACTTTCTTATCAATGATTTACAATTGTGTGAAATAA

mRNA sequence

ATGGCTTCTTCCAAAGCTCTCCAAATGATCTTCCCTTTCATAGGCCTTATATTTCTTTCCTTCTTCCCGTATCCAAATATATCGAATCCACTGATTTTGAAACACAATTCCCACGATATAAACTCATCTTCCTTAATATTTCTAAAGAAGAATCTCCAAGGATGTCGTTTGGGAGATACAATACAAGGAATTCATCAAATAAAAAAATACCTTCAACGTTTTGGTTACATTACCAATATCCAAAAGAACTCCAACCCAATATTTGATGACACTTTTGACCAAGTCTTGGAGTCCTCCCTTAAAACTTACCAAAAAAACCACAATCTTTTTGCCTCTGGAATCTTGGATTCCAACACCATTGCCCAAATGGCTATGCCTCGATGTGGGGTTGAAGATGTAATGAATGGAACAACTTGGACGAAATCAAGAAGAAGAAAAAGAAACCAAAATTACAGAAATGGCCATACGCATTTCCACAAAGTTTCTCACTTTGCTTTCTTTGAAGACAACCCAAAATGGCCAGCTTCAAAGTTTCACTTGAGCTATGCATTTCTTCGAGGCTACCCAACTGAAGCAATAATGGCAGTGTCAAGAGCTTTTTCAAAGTGGGCTTTCAATACCTATTTCACATTTTCACATGTTGTAGACTATAAGAAAGCTGATATCAAAATAAGCTTTGAGAGAGAAGAGCATGGAGATATTGATCCGTTTGATGGTACGGGAAAAGTTTTGGCCCATGCATTTCCACCAACGGATGGGAGACTTCATTTTGATGCTGATGAGGCTTGGTCTGTGGGAGCAATTTCAGGTTATTTTGATGTGGAAACTGTGGCTTTGCATGAGATTGGACATCTTCTTGGGCTTCAACATAGCTCCGTTCAAGGGGCTATCATGTTTCCAACTATACCTGAGGGAGAAACCAAGGGTTTGCATGGTGATGATATTGCTGGAATTAAGGCCTTATATGAATGGCTTCAAATTAATTTGATCTCTAAAATGTTTGTTGGCTTTACTTTCTTATCAATGATTTACAATTGTGTGAAATAA

Coding sequence (CDS)

ATGGCTTCTTCCAAAGCTCTCCAAATGATCTTCCCTTTCATAGGCCTTATATTTCTTTCCTTCTTCCCGTATCCAAATATATCGAATCCACTGATTTTGAAACACAATTCCCACGATATAAACTCATCTTCCTTAATATTTCTAAAGAAGAATCTCCAAGGATGTCGTTTGGGAGATACAATACAAGGAATTCATCAAATAAAAAAATACCTTCAACGTTTTGGTTACATTACCAATATCCAAAAGAACTCCAACCCAATATTTGATGACACTTTTGACCAAGTCTTGGAGTCCTCCCTTAAAACTTACCAAAAAAACCACAATCTTTTTGCCTCTGGAATCTTGGATTCCAACACCATTGCCCAAATGGCTATGCCTCGATGTGGGGTTGAAGATGTAATGAATGGAACAACTTGGACGAAATCAAGAAGAAGAAAAAGAAACCAAAATTACAGAAATGGCCATACGCATTTCCACAAAGTTTCTCACTTTGCTTTCTTTGAAGACAACCCAAAATGGCCAGCTTCAAAGTTTCACTTGAGCTATGCATTTCTTCGAGGCTACCCAACTGAAGCAATAATGGCAGTGTCAAGAGCTTTTTCAAAGTGGGCTTTCAATACCTATTTCACATTTTCACATGTTGTAGACTATAAGAAAGCTGATATCAAAATAAGCTTTGAGAGAGAAGAGCATGGAGATATTGATCCGTTTGATGGTACGGGAAAAGTTTTGGCCCATGCATTTCCACCAACGGATGGGAGACTTCATTTTGATGCTGATGAGGCTTGGTCTGTGGGAGCAATTTCAGGTTATTTTGATGTGGAAACTGTGGCTTTGCATGAGATTGGACATCTTCTTGGGCTTCAACATAGCTCCGTTCAAGGGGCTATCATGTTTCCAACTATACCTGAGGGAGAAACCAAGGGTTTGCATGGTGATGATATTGCTGGAATTAAGGCCTTATATGAATGGCTTCAAATTAATTTGATCTCTAAAATGTTTGTTGGCTTTACTTTCTTATCAATGATTTACAATTGTGTGAAATAA

Protein sequence

MASSKALQMIFPFIGLIFLSFFPYPNISNPLILKHNSHDINSSSLIFLKKNLQGCRLGDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNPKWPASKFHLSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIMFPTIPEGETKGLHGDDIAGIKALYEWLQINLISKMFVGFTFLSMIYNCVK

Homology

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BLAST of Lsi02G015300 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 120/283 (42.40%), Postives = 169/283 (59.72%), Query Frame = 0


Query: 51  NLQGCRLGDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLF 110
           N  GC  G    G++ +K+Y Q FGYIT    + N  F D FD +L+++++ YQ+N  L 
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGN--FTDDFDDILKNAVEMYQRNFQLN 107

Query: 111 ASGILDSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDN 170
            +G+LD  T+  + +PRCG  DV+NGT+   S R+    ++      FH V H++FF   
Sbjct: 108 VTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGE 167

Query: 171 PKWPASKFHLSYAF-LRGYPTEAIMAV-SRAFSKWAFNTYFTFSHVVDYKKADIKISFER 230
           P+WP ++  L+YAF  R   TE + +V SRAF++W   T  TF+ V  +  +DI I F  
Sbjct: 168 PRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYS 227

Query: 231 EEHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSVG--------AISGYFDVETVALH 290
            EHGD +PFDG  + LAHAF P  G  H D +E W V         ++S   D+E+VA+H
Sbjct: 228 GEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVH 287

Query: 291 EIGHLLGLQHSSVQGAIMFPTIPEGETK-GLHGDDIAGIKALY 323
           EIGHLLGL HSSV+G+IM+PTI  G  K  L  DD+ G++ LY
Sbjct: 288 EIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of Lsi02G015300 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.4e-56
Identity = 121/282 (42.91%), Postives = 166/282 (58.87%), Query Frame = 0


Query: 51  NLQGCRLGDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLF 110
           N  GC  G  + G+++IKKY QRFGYI      +   F D FD +L+++++ YQ N NL 
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGN---FTDDFDDILKAAVELYQTNFNLN 105

Query: 111 ASGILDSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDN 170
            +G LD+ TI  + +PRCG  DV+NGT+     RRK  +      TH H V  +  F   
Sbjct: 106 VTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE-VNFSRTHLHAVKRYTLFPGE 165

Query: 171 PKWPASKFHLSYAFLRGYP-TEAIMAV-SRAFSKWAFNTYFTFSHVVDYKKADIKISFER 230
           P+WP ++  L+YAF    P TE + +V SRAF +W+  T   F+    +  +DI I F  
Sbjct: 166 PRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYT 225

Query: 231 EEHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSVG-------AISGYFDVETVALHE 290
            +HGD +PFDG    LAHAF P  G+ H DADE W V        +++   D+E+VA+HE
Sbjct: 226 GDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHE 285

Query: 291 IGHLLGLQHSSVQGAIMFPTIPEGETK-GLHGDDIAGIKALY 323
           IGHLLGL HSSV+ +IM+PTI  G+ K  L  DD+ GI+ LY
Sbjct: 286 IGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

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BLAST of Lsi02G015300 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 1.4e-54
Identity = 120/274 (43.80%), Postives = 152/274 (55.47%), Query Frame = 0


Query: 58  GDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDS 117
           G   +G+  +K Y    GYI N      P FDD FD  L S++KTYQKN+NL  +G  D 
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNA-----PHFDDNFDDTLVSAIKTYQKNYNLNVTGKFDI 107

Query: 118 NTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNPKWPASK 177
           NT+ Q+  PRCGV D++  T               N  T F  +S + FF+D P+W A  
Sbjct: 108 NTLKQIMTPRCGVPDIIINT---------------NKTTSFGMISDYTFFKDMPRWQAGT 167

Query: 178 FHLSYAFLRGYPTEAIM--AVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDID 237
             L+YAF      +     A++RAFSKW       F     Y+ A+IKI F  + HGD  
Sbjct: 168 TQLTYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPY 227

Query: 238 PFDGTGKVLAHAFPPTDGRLHFDADEAW------SVGAISGYFDVETVALHEIGHLLGLQ 297
           PFDG G +L HAF PTDGR HFDADE W      +   ++  FD+E+VA+HEIGHLLGL 
Sbjct: 228 PFDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLG 287

Query: 298 HSSVQGAIMFPTIPEGETK-GLHGDDIAGIKALY 323
           HSS   AIM+P+IP    K  L  DDI GI+ LY
Sbjct: 288 HSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of Lsi02G015300 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.9e-51
Identity = 118/283 (41.70%), Postives = 155/283 (54.77%), Query Frame = 0


Query: 52  LQGCRLGDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFA 111
           L GC +G+ I G+ ++K+Y +RFGYIT     +     D FD VL+S++ TYQKN NL  
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCT-----DDFDDVLQSAINTYQKNFNLKV 107

Query: 112 SGILDSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNP 171
           +G LDS+T+ Q+  PRCG  D+++G             +  NG         ++FF   P
Sbjct: 108 TGKLDSSTLRQIVKPRCGNPDLIDGV------------SEMNGGKILRTTEKYSFFPGKP 167

Query: 172 KWPASKFHLSYAFL--RGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFERE 231
           +WP  K  L+YAF        E     SRAF++WA  T   F+      +ADI I F   
Sbjct: 168 RWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSG 227

Query: 232 EHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSVG---------AISGYFDVETVALH 291
           EHGD +PFDG    LAHA  P  G LH D DE W +           ++   D+E+VA+H
Sbjct: 228 EHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVH 287

Query: 292 EIGHLLGLQHSSVQGAIMFPTIPEGETK-GLHGDDIAGIKALY 323
           EIGHLLGL HSSV+ AIMFP I  G+ K  L  DDI GI+ LY
Sbjct: 288 EIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of Lsi02G015300 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.7e-45
Identity = 104/279 (37.28%), Postives = 154/279 (55.20%), Query Frame = 0


Query: 56  RLGDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGIL 115
           ++G  + G+ ++K+YL RFGY+     + + IF D FD  LES++  YQ+N  L  +G L
Sbjct: 62  QIGSHVSGVSELKRYLHRFGYV----NDGSEIFSDVFDGPLESAISLYQENLGLPITGRL 121

Query: 116 DSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNPKWPA 175
           D++T+  M++PRCGV D                 +    +   H  +H+ +F   PKW  
Sbjct: 122 DTSTVTLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW-- 181

Query: 176 SKFHLSYAFLRGYPTEAIMA------VSRAFSKWAFNTYFTFSHVVDYKKADIKISFERE 235
           ++  L+YA  + +  + + +        RAFS+W+     +F  V D+  AD+KI F   
Sbjct: 182 NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAG 241

Query: 236 EHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSV-----GAISGYFDVETVALHEIGH 295
           +HGD  PFDG    LAHAF P +GRLH DA E W V     G+     D+E+VA HEIGH
Sbjct: 242 DHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGH 301

Query: 296 LLGLQHSSVQGAIMFPTI-PEGETKGLHGDDIAGIKALY 323
           LLGL HSS + A+M+P++ P  +   L  DD+AG+  LY
Sbjct: 302 LLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Lsi02G015300 vs. ExPASy TrEMBL
Match: A0A1S3C814 (metalloendoproteinase 1-like OS=Cucumis melo OX=3656 GN=LOC103498042 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 3.1e-137
Identity = 247/324 (76.23%), Postives = 275/324 (84.88%), Query Frame = 0


Query: 1   MASSKALQMIFPFIGLIFLSFFPYPNISNPLILKHNSHDINSSSLIFLKKNLQGCRLGDT 60
           MASSKA+Q+IFPF  L+FLS FP  N S+P+ILKHNSH++NSS+ +   KNLQGC LGDT
Sbjct: 1   MASSKAIQIIFPFT-LLFLSLFPNLNTSSPMILKHNSHNMNSSNSLMFLKNLQGCHLGDT 60

Query: 61  IQGIHQIKKYLQRFGYI-TNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNT 120
            QGI QIKKYLQRFGYI TNIQK+SNPIFDDTFDQVLES+LKTYQ NHNL  SGILDSNT
Sbjct: 61  KQGIQQIKKYLQRFGYITTNIQKHSNPIFDDTFDQVLESALKTYQTNHNLAPSGILDSNT 120

Query: 121 IAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYR-NGHTHFHKVSHFAFFEDNPKWPASKF 180
           +AQ+A PRCGV+DV+        + +KRNQNY+ NGH HFHKVSHF FFE N KWP+SKF
Sbjct: 121 LAQIAKPRCGVQDVI--------KNKKRNQNYKNNGHIHFHKVSHFTFFEGNLKWPSSKF 180

Query: 181 HLSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFD 240
           HLSY FL  YP EAI  VSRAFSKW+ NT+F FSHVVDY+KADIKISFER EHGD  PFD
Sbjct: 181 HLSYGFLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVVDYRKADIKISFERGEHGDNAPFD 240

Query: 241 GTGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIM 300
           G G VLAHA+PPTDGRLHFD DEAWSVGAISGYFDVETVALHEIGH+LGLQHS+++ AIM
Sbjct: 241 GVGGVLAHAYPPTDGRLHFDGDEAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIM 300

Query: 301 FPTIPEGETKGLHGDDIAGIKALY 323
           FP+IPEG TKGLHGDDIAGIK LY
Sbjct: 301 FPSIPEGVTKGLHGDDIAGIKTLY 315

[Close]

BLAST of Lsi02G015300 vs. ExPASy TrEMBL
Match: A0A5D3BM88 (Metalloendoproteinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00220 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.5e-136
Identity = 246/324 (75.93%), Postives = 274/324 (84.57%), Query Frame = 0


Query: 1   MASSKALQMIFPFIGLIFLSFFPYPNISNPLILKHNSHDINSSSLIFLKKNLQGCRLGDT 60
           MASSKA+Q+IFPF  L+FLS FP  N S+P+ILKHNSH++NSS+ +   KNLQGC LGDT
Sbjct: 1   MASSKAIQIIFPFT-LLFLSLFPNLNTSSPMILKHNSHNMNSSNSLMFLKNLQGCHLGDT 60

Query: 61  IQGIHQIKKYLQRFGYI-TNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNT 120
            QGI QIKKYLQRFGYI TNIQK+SNPIFDDTFDQVLES+LKTYQ NHNL  SGILDSNT
Sbjct: 61  KQGIQQIKKYLQRFGYITTNIQKHSNPIFDDTFDQVLESALKTYQTNHNLAPSGILDSNT 120

Query: 121 IAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYR-NGHTHFHKVSHFAFFEDNPKWPASKF 180
           +AQ+A PRCGV+DV+        + +KRNQ Y+ NGH HFHKVSHF FFE N KWP+SKF
Sbjct: 121 LAQIAKPRCGVQDVI--------KNKKRNQKYKNNGHIHFHKVSHFTFFEGNLKWPSSKF 180

Query: 181 HLSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFD 240
           HLSY FL  YP EAI  VSRAFSKW+ NT+F FSHVVDY+KADIKISFER EHGD  PFD
Sbjct: 181 HLSYGFLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVVDYRKADIKISFERGEHGDNAPFD 240

Query: 241 GTGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIM 300
           G G VLAHA+PPTDGRLHFD DEAWSVGAISGYFDVETVALHEIGH+LGLQHS+++ AIM
Sbjct: 241 GVGGVLAHAYPPTDGRLHFDGDEAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIM 300

Query: 301 FPTIPEGETKGLHGDDIAGIKALY 323
           FP+IPEG TKGLHGDDIAGIK LY
Sbjct: 301 FPSIPEGVTKGLHGDDIAGIKTLY 315

[Close]

BLAST of Lsi02G015300 vs. ExPASy TrEMBL
Match: Q9LEL9 (Matrix metalloproteinase OS=Cucumis sativus OX=3659 GN=MMP-1 PE=2 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.2e-135
Identity = 243/324 (75.00%), Postives = 273/324 (84.26%), Query Frame = 0


Query: 1   MASSKALQMIFPFIGLIFLSFFPYPNISNPLILKHNSHDINSSSLIFLKKNLQGCRLGDT 60
           MAS KALQ+IFPF  L+FLS FP PN S+P+ILKH+S ++NSS+ +   KNLQGC LGDT
Sbjct: 1   MASPKALQIIFPFT-LLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDT 60

Query: 61  IQGIHQIKKYLQRFGYI-TNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNT 120
            QGIHQIKKYLQRFGYI TNIQK+SNPIFDDTFD +LES+LKTYQ NHNL  SGILDSNT
Sbjct: 61  KQGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNT 120

Query: 121 IAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNY-RNGHTHFHKVSHFAFFEDNPKWPASKF 180
           IAQ+AMPRCGV+DV+        + +KRNQN+  NGHTHFHKVSHF FFE N KWP+SK 
Sbjct: 121 IAQIAMPRCGVQDVIK-----NKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKL 180

Query: 181 HLSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFD 240
           HLSY FL  YP +AI  VSRAFSKW+ NT+F FSHV DY+KADIKISFER EHGD  PFD
Sbjct: 181 HLSYGFLPNYPIDAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFD 240

Query: 241 GTGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIM 300
           G G VLAHA+ PTDGRLHFD D+AWSVGAISGYFDVETVALHEIGH+LGLQHS+++ AIM
Sbjct: 241 GVGGVLAHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIM 300

Query: 301 FPTIPEGETKGLHGDDIAGIKALY 323
           FP+IPEG TKGLHGDDIAGIKALY
Sbjct: 301 FPSIPEGVTKGLHGDDIAGIKALY 318

[Close]

BLAST of Lsi02G015300 vs. ExPASy TrEMBL
Match: A0A6J1BXD5 (metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.2e-127
Identity = 233/323 (72.14%), Postives = 269/323 (83.28%), Query Frame = 0


Query: 3   SSKALQMIFPFIGLIFLSFFPYPNI-SNPLILKHN--SHDINSSSLIFLKKNLQGCRLGD 62
           +SKALQ IF FI  + LSFFP+ N+ S+ LILK N  +HD+NSSS +    NL+GCRLGD
Sbjct: 2   ASKALQ-IFAFILFLLLSFFPHLNMSSHVLILKQNAIAHDMNSSSSLSF-NNLRGCRLGD 61

Query: 63  TIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNT 122
           T QGIH++KKYL+RFGYITN++KNS  + DDTFD  LES++KTYQKN+N+ ASGILD+ T
Sbjct: 62  TAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKT 121

Query: 123 IAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNPKWPASKFH 182
           I QM MPRCGV+D +NGTTW +S  R+RNQ   NGH+HFHKVSHFAFFE N KWPASKFH
Sbjct: 122 ITQMGMPRCGVQDFINGTTWMRSSGRRRNQ---NGHSHFHKVSHFAFFEGNLKWPASKFH 181

Query: 183 LSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFDG 242
           LSYAFL GYP+EAI+ VSRAFSKWA NT+FTFSHV D+K ADIKISFE EEHGD   FDG
Sbjct: 182 LSYAFLPGYPSEAILPVSRAFSKWASNTHFTFSHVSDHKTADIKISFEIEEHGDDAAFDG 241

Query: 243 TGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIMF 302
            G +LAHA+PPTDGR H DA E WSVGA+SG+ DVETVALHEIGHLLGLQHSS++G IMF
Sbjct: 242 AGGILAHAYPPTDGRFHLDAHERWSVGAVSGHHDVETVALHEIGHLLGLQHSSIEGPIMF 301

Query: 303 PTIPEGETKGLHGDDIAGIKALY 323
           P+I EG TKGLHGDDIAGIKALY
Sbjct: 302 PSISEGVTKGLHGDDIAGIKALY 319

[Close]

BLAST of Lsi02G015300 vs. ExPASy TrEMBL
Match: A0A6J1HFG1 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 3.5e-125
Identity = 231/335 (68.96%), Postives = 266/335 (79.40%), Query Frame = 0


Query: 3   SSKALQMIFPFIGLIFLSFFPYPNI-------------SNPLILKHNSHDINSSSLIFLK 62
           +SK LQ++     L+  SFFP+ +I             S+P+IL HNSH  NSSSL+FL 
Sbjct: 2   ASKPLQLL--AFTLLLPSFFPHFDIVSVQCHSESEAVGSSPIILNHNSHATNSSSLLFL- 61

Query: 63  KNLQGCRLGDTIQGIHQIKKYLQRFGYITNIQKNSNPI-FDDTFDQVLESSLKTYQKNHN 122
            NLQGCRLGDT QGIH+IKKYLQRFGYITN+QK SN +  DDTFD  LES+LKTYQKN N
Sbjct: 62  NNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNFN 121

Query: 123 LFASGILDSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFE 182
           L ASGILD+NT+AQM  PRCGVEDV+NGTTW +S R ++ +     H HFH VSHFAFFE
Sbjct: 122 LVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKM----HAHFHLVSHFAFFE 181

Query: 183 DNPKWPASKFHLSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFER 242
            N KWP SKFHL+YAFL GYP EA+MAVSRAFSKWAFNT+FTFS V D+ KADIKISFE 
Sbjct: 182 GNLKWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEI 241

Query: 243 EEHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGL 302
            +HGD  PFDG G +LAHA+ PTDGRLHFD DEAWSVGA+ GYFDVET+ALHEIGH+LGL
Sbjct: 242 GDHGDDVPFDGVGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGL 301

Query: 303 QHSSVQGAIMFPTIPEGETKGLHGDDIAGIKALYE 324
           QHSS++ AIMFP+I EG TKGLHGDDIAGI+ALY+
Sbjct: 302 QHSSIENAIMFPSISEGVTKGLHGDDIAGIRALYK 329

[Close]

BLAST of Lsi02G015300 vs. NCBI nr
Match: XP_038891016.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 533.9 bits (1374), Expect = 1.1e-147
Identity = 263/323 (81.42%), Postives = 287/323 (88.85%), Query Frame = 0


Query: 1   MASSKALQMIFPFIGLIFLSFFPYPNISNPLILKHNSHDINSSSLIFLKKNLQGCRLGDT 60
           MASSKALQMIFPF  L+ L FFP+PNIS P+ILKHNSH++N+SSL+FLKK+LQGCR+GDT
Sbjct: 1   MASSKALQMIFPFT-LLLLFFFPHPNISTPMILKHNSHNMNASSLMFLKKDLQGCRVGDT 60

Query: 61  IQGIHQIKKYLQRFGYITNIQKNSN-PIFDDTFDQVLESSLKTYQKNHNLFASGILDSNT 120
           IQGIHQIKKYLQRFGYITNI+K+SN PIFDDTFD +LES+LKTYQKNHNL  SGILDSNT
Sbjct: 61  IQGIHQIKKYLQRFGYITNIKKHSNYPIFDDTFDHILESALKTYQKNHNLVPSGILDSNT 120

Query: 121 IAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNPKWPASKFH 180
           IAQMAMPRCGV+DV+NGTT      RKRNQ Y+NGHTHFHKVSHF  FE NPKWP+SKFH
Sbjct: 121 IAQMAMPRCGVQDVINGTT------RKRNQIYKNGHTHFHKVSHFTLFEGNPKWPSSKFH 180

Query: 181 LSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFDG 240
           LSYAF  GYP+EAIMAVSRAFSKWA NT+FTFSHVVDY KADIKISFER EHGD   FDG
Sbjct: 181 LSYAFPPGYPSEAIMAVSRAFSKWALNTHFTFSHVVDYNKADIKISFERGEHGDDASFDG 240

Query: 241 TGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIMF 300
            G +LAHA+PPTDGRLHFDADE WSVGAISGYFDVETVALHEIGH+LGLQHSS+  AIMF
Sbjct: 241 VGGILAHAYPPTDGRLHFDADEPWSVGAISGYFDVETVALHEIGHILGLQHSSIGTAIMF 300

Query: 301 PTIPEGETKGLHGDDIAGIKALY 323
           P+I  G TKGLH DDIAGIKALY
Sbjct: 301 PSISAGVTKGLHWDDIAGIKALY 316

[Close]

BLAST of Lsi02G015300 vs. NCBI nr
Match: XP_008458715.1 (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 498.0 bits (1281), Expect = 6.4e-137
Identity = 247/324 (76.23%), Postives = 275/324 (84.88%), Query Frame = 0


Query: 1   MASSKALQMIFPFIGLIFLSFFPYPNISNPLILKHNSHDINSSSLIFLKKNLQGCRLGDT 60
           MASSKA+Q+IFPF  L+FLS FP  N S+P+ILKHNSH++NSS+ +   KNLQGC LGDT
Sbjct: 1   MASSKAIQIIFPFT-LLFLSLFPNLNTSSPMILKHNSHNMNSSNSLMFLKNLQGCHLGDT 60

Query: 61  IQGIHQIKKYLQRFGYI-TNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNT 120
            QGI QIKKYLQRFGYI TNIQK+SNPIFDDTFDQVLES+LKTYQ NHNL  SGILDSNT
Sbjct: 61  KQGIQQIKKYLQRFGYITTNIQKHSNPIFDDTFDQVLESALKTYQTNHNLAPSGILDSNT 120

Query: 121 IAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYR-NGHTHFHKVSHFAFFEDNPKWPASKF 180
           +AQ+A PRCGV+DV+        + +KRNQNY+ NGH HFHKVSHF FFE N KWP+SKF
Sbjct: 121 LAQIAKPRCGVQDVI--------KNKKRNQNYKNNGHIHFHKVSHFTFFEGNLKWPSSKF 180

Query: 181 HLSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFD 240
           HLSY FL  YP EAI  VSRAFSKW+ NT+F FSHVVDY+KADIKISFER EHGD  PFD
Sbjct: 181 HLSYGFLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVVDYRKADIKISFERGEHGDNAPFD 240

Query: 241 GTGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIM 300
           G G VLAHA+PPTDGRLHFD DEAWSVGAISGYFDVETVALHEIGH+LGLQHS+++ AIM
Sbjct: 241 GVGGVLAHAYPPTDGRLHFDGDEAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIM 300

Query: 301 FPTIPEGETKGLHGDDIAGIKALY 323
           FP+IPEG TKGLHGDDIAGIK LY
Sbjct: 301 FPSIPEGVTKGLHGDDIAGIKTLY 315

[Close]

BLAST of Lsi02G015300 vs. NCBI nr
Match: KAA0037438.1 (metalloendoproteinase 1-like [Cucumis melo var. makuwa] >TYK00228.1 metalloendoproteinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 495.7 bits (1275), Expect = 3.2e-136
Identity = 246/324 (75.93%), Postives = 274/324 (84.57%), Query Frame = 0


Query: 1   MASSKALQMIFPFIGLIFLSFFPYPNISNPLILKHNSHDINSSSLIFLKKNLQGCRLGDT 60
           MASSKA+Q+IFPF  L+FLS FP  N S+P+ILKHNSH++NSS+ +   KNLQGC LGDT
Sbjct: 1   MASSKAIQIIFPFT-LLFLSLFPNLNTSSPMILKHNSHNMNSSNSLMFLKNLQGCHLGDT 60

Query: 61  IQGIHQIKKYLQRFGYI-TNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNT 120
            QGI QIKKYLQRFGYI TNIQK+SNPIFDDTFDQVLES+LKTYQ NHNL  SGILDSNT
Sbjct: 61  KQGIQQIKKYLQRFGYITTNIQKHSNPIFDDTFDQVLESALKTYQTNHNLAPSGILDSNT 120

Query: 121 IAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYR-NGHTHFHKVSHFAFFEDNPKWPASKF 180
           +AQ+A PRCGV+DV+        + +KRNQ Y+ NGH HFHKVSHF FFE N KWP+SKF
Sbjct: 121 LAQIAKPRCGVQDVI--------KNKKRNQKYKNNGHIHFHKVSHFTFFEGNLKWPSSKF 180

Query: 181 HLSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFD 240
           HLSY FL  YP EAI  VSRAFSKW+ NT+F FSHVVDY+KADIKISFER EHGD  PFD
Sbjct: 181 HLSYGFLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVVDYRKADIKISFERGEHGDNAPFD 240

Query: 241 GTGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIM 300
           G G VLAHA+PPTDGRLHFD DEAWSVGAISGYFDVETVALHEIGH+LGLQHS+++ AIM
Sbjct: 241 GVGGVLAHAYPPTDGRLHFDGDEAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIM 300

Query: 301 FPTIPEGETKGLHGDDIAGIKALY 323
           FP+IPEG TKGLHGDDIAGIK LY
Sbjct: 301 FPSIPEGVTKGLHGDDIAGIKTLY 315

[Close]

BLAST of Lsi02G015300 vs. NCBI nr
Match: NP_001267680.1 (metalloendoproteinase 1-like precursor [Cucumis sativus] >KGN52412.1 hypothetical protein Csa_009090 [Cucumis sativus] >CAB76364.1 matrix metalloproteinase [Cucumis sativus])

HSP 1 Score: 491.9 bits (1265), Expect = 4.6e-135
Identity = 243/324 (75.00%), Postives = 273/324 (84.26%), Query Frame = 0


Query: 1   MASSKALQMIFPFIGLIFLSFFPYPNISNPLILKHNSHDINSSSLIFLKKNLQGCRLGDT 60
           MAS KALQ+IFPF  L+FLS FP PN S+P+ILKH+S ++NSS+ +   KNLQGC LGDT
Sbjct: 1   MASPKALQIIFPFT-LLFLSLFPNPNTSSPIILKHSSQNMNSSNSLMFLKNLQGCHLGDT 60

Query: 61  IQGIHQIKKYLQRFGYI-TNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNT 120
            QGIHQIKKYLQRFGYI TNIQK+SNPIFDDTFD +LES+LKTYQ NHNL  SGILDSNT
Sbjct: 61  KQGIHQIKKYLQRFGYITTNIQKHSNPIFDDTFDHILESALKTYQTNHNLAPSGILDSNT 120

Query: 121 IAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNY-RNGHTHFHKVSHFAFFEDNPKWPASKF 180
           IAQ+AMPRCGV+DV+        + +KRNQN+  NGHTHFHKVSHF FFE N KWP+SK 
Sbjct: 121 IAQIAMPRCGVQDVIK-----NKKTKKRNQNFTNNGHTHFHKVSHFTFFEGNLKWPSSKL 180

Query: 181 HLSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFD 240
           HLSY FL  YP +AI  VSRAFSKW+ NT+F FSHV DY+KADIKISFER EHGD  PFD
Sbjct: 181 HLSYGFLPNYPIDAIKPVSRAFSKWSLNTHFKFSHVADYRKADIKISFERGEHGDNAPFD 240

Query: 241 GTGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIM 300
           G G VLAHA+ PTDGRLHFD D+AWSVGAISGYFDVETVALHEIGH+LGLQHS+++ AIM
Sbjct: 241 GVGGVLAHAYAPTDGRLHFDGDDAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIM 300

Query: 301 FPTIPEGETKGLHGDDIAGIKALY 323
           FP+IPEG TKGLHGDDIAGIKALY
Sbjct: 301 FPSIPEGVTKGLHGDDIAGIKALY 318

[Close]

BLAST of Lsi02G015300 vs. NCBI nr
Match: XP_022134170.1 (metalloendoproteinase 2-MMP-like [Momordica charantia])

HSP 1 Score: 465.3 bits (1196), Expect = 4.6e-127
Identity = 233/323 (72.14%), Postives = 269/323 (83.28%), Query Frame = 0


Query: 3   SSKALQMIFPFIGLIFLSFFPYPNI-SNPLILKHN--SHDINSSSLIFLKKNLQGCRLGD 62
           +SKALQ IF FI  + LSFFP+ N+ S+ LILK N  +HD+NSSS +    NL+GCRLGD
Sbjct: 2   ASKALQ-IFAFILFLLLSFFPHLNMSSHVLILKQNAIAHDMNSSSSLSF-NNLRGCRLGD 61

Query: 63  TIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNT 122
           T QGIH++KKYL+RFGYITN++KNS  + DDTFD  LES++KTYQKN+N+ ASGILD+ T
Sbjct: 62  TAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKT 121

Query: 123 IAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNPKWPASKFH 182
           I QM MPRCGV+D +NGTTW +S  R+RNQ   NGH+HFHKVSHFAFFE N KWPASKFH
Sbjct: 122 ITQMGMPRCGVQDFINGTTWMRSSGRRRNQ---NGHSHFHKVSHFAFFEGNLKWPASKFH 181

Query: 183 LSYAFLRGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDPFDG 242
           LSYAFL GYP+EAI+ VSRAFSKWA NT+FTFSHV D+K ADIKISFE EEHGD   FDG
Sbjct: 182 LSYAFLPGYPSEAILPVSRAFSKWASNTHFTFSHVSDHKTADIKISFEIEEHGDDAAFDG 241

Query: 243 TGKVLAHAFPPTDGRLHFDADEAWSVGAISGYFDVETVALHEIGHLLGLQHSSVQGAIMF 302
            G +LAHA+PPTDGR H DA E WSVGA+SG+ DVETVALHEIGHLLGLQHSS++G IMF
Sbjct: 242 AGGILAHAYPPTDGRFHLDAHERWSVGAVSGHHDVETVALHEIGHLLGLQHSSIEGPIMF 301

Query: 303 PTIPEGETKGLHGDDIAGIKALY 323
           P+I EG TKGLHGDDIAGIKALY
Sbjct: 302 PSISEGVTKGLHGDDIAGIKALY 319

[Close]

BLAST of Lsi02G015300 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 228.4 bits (581), Expect = 8.8e-60
Identity = 120/283 (42.40%), Postives = 169/283 (59.72%), Query Frame = 0


Query: 51  NLQGCRLGDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLF 110
           N  GC  G    G++ +K+Y Q FGYIT    + N  F D FD +L+++++ YQ+N  L 
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGN--FTDDFDDILKNAVEMYQRNFQLN 107

Query: 111 ASGILDSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDN 170
            +G+LD  T+  + +PRCG  DV+NGT+   S R+    ++      FH V H++FF   
Sbjct: 108 VTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGE 167

Query: 171 PKWPASKFHLSYAF-LRGYPTEAIMAV-SRAFSKWAFNTYFTFSHVVDYKKADIKISFER 230
           P+WP ++  L+YAF  R   TE + +V SRAF++W   T  TF+ V  +  +DI I F  
Sbjct: 168 PRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYS 227

Query: 231 EEHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSVG--------AISGYFDVETVALH 290
            EHGD +PFDG  + LAHAF P  G  H D +E W V         ++S   D+E+VA+H
Sbjct: 228 GEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVH 287

Query: 291 EIGHLLGLQHSSVQGAIMFPTIPEGETK-GLHGDDIAGIKALY 323
           EIGHLLGL HSSV+G+IM+PTI  G  K  L  DD+ G++ LY
Sbjct: 288 EIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]

BLAST of Lsi02G015300 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 220.3 bits (560), Expect = 2.4e-57
Identity = 121/282 (42.91%), Postives = 166/282 (58.87%), Query Frame = 0


Query: 51  NLQGCRLGDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLF 110
           N  GC  G  + G+++IKKY QRFGYI      +   F D FD +L+++++ YQ N NL 
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGN---FTDDFDDILKAAVELYQTNFNLN 105

Query: 111 ASGILDSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDN 170
            +G LD+ TI  + +PRCG  DV+NGT+     RRK  +      TH H V  +  F   
Sbjct: 106 VTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE-VNFSRTHLHAVKRYTLFPGE 165

Query: 171 PKWPASKFHLSYAFLRGYP-TEAIMAV-SRAFSKWAFNTYFTFSHVVDYKKADIKISFER 230
           P+WP ++  L+YAF    P TE + +V SRAF +W+  T   F+    +  +DI I F  
Sbjct: 166 PRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYT 225

Query: 231 EEHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSVG-------AISGYFDVETVALHE 290
            +HGD +PFDG    LAHAF P  G+ H DADE W V        +++   D+E+VA+HE
Sbjct: 226 GDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHE 285

Query: 291 IGHLLGLQHSSVQGAIMFPTIPEGETK-GLHGDDIAGIKALY 323
           IGHLLGL HSSV+ +IM+PTI  G+ K  L  DD+ GI+ LY
Sbjct: 286 IGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]

BLAST of Lsi02G015300 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.4e-52
Identity = 118/283 (41.70%), Postives = 155/283 (54.77%), Query Frame = 0


Query: 52  LQGCRLGDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFA 111
           L GC +G+ I G+ ++K+Y +RFGYIT     +     D FD VL+S++ TYQKN NL  
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCT-----DDFDDVLQSAINTYQKNFNLKV 107

Query: 112 SGILDSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNP 171
           +G LDS+T+ Q+  PRCG  D+++G             +  NG         ++FF   P
Sbjct: 108 TGKLDSSTLRQIVKPRCGNPDLIDGV------------SEMNGGKILRTTEKYSFFPGKP 167

Query: 172 KWPASKFHLSYAFL--RGYPTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFERE 231
           +WP  K  L+YAF        E     SRAF++WA  T   F+      +ADI I F   
Sbjct: 168 RWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSG 227

Query: 232 EHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSVG---------AISGYFDVETVALH 291
           EHGD +PFDG    LAHA  P  G LH D DE W +           ++   D+E+VA+H
Sbjct: 228 EHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVH 287

Query: 292 EIGHLLGLQHSSVQGAIMFPTIPEGETK-GLHGDDIAGIKALY 323
           EIGHLLGL HSSV+ AIMFP I  G+ K  L  DDI GI+ LY
Sbjct: 288 EIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of Lsi02G015300 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.9e-46
Identity = 104/279 (37.28%), Postives = 154/279 (55.20%), Query Frame = 0


Query: 56  RLGDTIQGIHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGIL 115
           ++G  + G+ ++K+YL RFGY+     + + IF D FD  LES++  YQ+N  L  +G L
Sbjct: 62  QIGSHVSGVSELKRYLHRFGYV----NDGSEIFSDVFDGPLESAISLYQENLGLPITGRL 121

Query: 116 DSNTIAQMAMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNPKWPA 175
           D++T+  M++PRCGV D                 +    +   H  +H+ +F   PKW  
Sbjct: 122 DTSTVTLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW-- 181

Query: 176 SKFHLSYAFLRGYPTEAIMA------VSRAFSKWAFNTYFTFSHVVDYKKADIKISFERE 235
           ++  L+YA  + +  + + +        RAFS+W+     +F  V D+  AD+KI F   
Sbjct: 182 NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAG 241

Query: 236 EHGDIDPFDGTGKVLAHAFPPTDGRLHFDADEAWSV-----GAISGYFDVETVALHEIGH 295
           +HGD  PFDG    LAHAF P +GRLH DA E W V     G+     D+E+VA HEIGH
Sbjct: 242 DHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGH 301

Query: 296 LLGLQHSSVQGAIMFPTI-PEGETKGLHGDDIAGIKALY 323
           LLGL HSS + A+M+P++ P  +   L  DD+AG+  LY
Sbjct: 302 LLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Lsi02G015300 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 166.0 bits (419), Expect = 5.4e-41
Identity = 104/271 (38.38%), Postives = 143/271 (52.77%), Query Frame = 0


Query: 64  IHQIKKYLQRFGYITNIQKNSNPIFDDTFDQVLESSLKTYQKNHNLFASGILDSNTIAQM 123
           I +IK++LQ++GY+        P   ++ D   E +L  YQKN  L  +G  DS+T++Q+
Sbjct: 50  IPEIKRHLQQYGYL--------PQNKESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQI 109

Query: 124 AMPRCGVEDVMNGTTWTKSRRRKRNQNYRNGHTHFHKVSHFAFFEDNPKWPAS-KFHLSY 183
            +PRCG  D +   T                   FH    + +F   P+W       L+Y
Sbjct: 110 LLPRCGFPDDVEPKT-----------------APFHTGKKYVYFPGRPRWTRDVPLKLTY 169

Query: 184 AF----LRGY--PTEAIMAVSRAFSKWAFNTYFTFSHVVDYKKADIKISFEREEHGDIDP 243
           AF    L  Y  PT+      RAF KWA     +F    DY  ADIKI F   +HGD +P
Sbjct: 170 AFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEP 229

Query: 244 FDGTGKVLAHAFPPTDGRLHFDADEAWSVG----AISGYFDVETVALHEIGHLLGLQHSS 303
           FDG   VLAH F P +GRLH D  E W+V       S   D+E+VA+HEIGH+LGL HSS
Sbjct: 230 FDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSS 289

Query: 304 VQGAIMFPTI-PEGETKGLHGDDIAGIKALY 323
           V+ A M+PT+ P  +   L+ DD+ G+++LY
Sbjct: 290 VKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

The following BLAST results are available for this feature:

BLAST of Lsi02G015300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XF51	1.2e-58	42.40	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
O04529	3.4e-56	42.91	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
P29136	1.4e-54	43.80	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q9ZUJ5	1.9e-51	41.70	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
O23507	2.7e-45	37.28	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of Lsi02G015300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C814	3.1e-137	76.23	metalloendoproteinase 1-like OS=Cucumis melo OX=3656 GN=LOC103498042 PE=3 SV=1	[more]
A0A5D3BM88	1.5e-136	75.93	Metalloendoproteinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
Q9LEL9	2.2e-135	75.00	Matrix metalloproteinase OS=Cucumis sativus OX=3659 GN=MMP-1 PE=2 SV=1	[more]
A0A6J1BXD5	2.2e-127	72.14	metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 ...	[more]
A0A6J1HFG1	3.5e-125	68.96	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 P...	[more]

BLAST of Lsi02G015300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891016.1	1.1e-147	81.42	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]
XP_008458715.1	6.4e-137	76.23	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
KAA0037438.1	3.2e-136	75.93	metalloendoproteinase 1-like [Cucumis melo var. makuwa] >TYK00228.1 metalloendop...	[more]
NP_001267680.1	4.6e-135	75.00	metalloendoproteinase 1-like precursor [Cucumis sativus] >KGN52412.1 hypothetica...	[more]
XP_022134170.1	4.6e-127	72.14	metalloendoproteinase 2-MMP-like [Momordica charantia]	[more]

BLAST of Lsi02G015300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24140.1	8.8e-60	42.40	Matrixin family protein	[more]
AT1G70170.1	2.4e-57	42.91	matrix metalloproteinase	[more]
AT1G59970.1	1.4e-52	41.70	Matrixin family protein	[more]
AT4G16640.1	1.9e-46	37.28	Matrixin family protein	[more]
AT2G45040.1	5.4e-41	38.38	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 277..302 score: 58.35 coord: 220..248 score: 59.97 coord: 123..136 score: 52.96 coord: 196..211 score: 34.68 coord: 310..323 score: 52.79
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 169..324 e-value: 5.3E-25 score: 99.1
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 57..325 e-value: 3.3E-70 score: 238.3
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 191..322 e-value: 1.1E-43 score: 148.9
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 62..123 e-value: 1.9E-11 score: 44.0
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 15..323
None	No IPR available	PANTHER	PTHR10201:SF269	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 15..323
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 166..323
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 172..322 e-value: 3.17324E-58 score: 183.559
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 55..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G015300.1	Lsi02G015300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity