Lsi02G014800 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G014800
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Defective in cullin neddylation protein
Location	chr02: 20541185 .. 20544700 (+)
RNA-Seq Expression	Lsi02G014800
Synteny	Lsi02G014800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTCCAGTCTGATGTGCATCGCGTTAGGAACTAAAACGGCTAACTTGTTTGTAGATATTCGATCTCAAAATGGCTTTTGTCATGGGAATGAAGACTCTGGACATGTTGAAACACAAATGGCTAAAAGTTCAAAGCAGGCACTTGCAGAGCTCTTAATATATGTGCAATCATCACTGCAGATGGGGTACCCTCAAAACTGATACTGTATACCTTGCGATGTTTTGTTAGTTGTACTCTGTGTCAAATGGACCATGATAGGCTAGGATATACAGAGTTTACTCTATTTTATACAACTCAATATCTTTTGTCCTTCCAAAATAAGATATTTTCCAACATTTTTTAACTGACAGATATATGAAACTATGATAGTTTATCAACTCAATTATCAAATGAAAATAATTTTTAACATCGTAGATGTGGATGGTTGCTAGTTTGATTTCATTTAGTTCTGGTTTGGTCTAGTATTTTCTTTCAGTTCATTAAAAAGGAATGTTACATGGAGAAAAACTAGACATGCCAAATTTACAACTGGATAAATGAGGCCATCCTATCATTTTTGTACTTCTGTAGCTTTACATCATAAGAAATAGCAGTTGATTACAGTATAGAAGTTTTTCTTATATTAATCTAGGTTATCTGAAAAGTGTAAATTCCGTTTGCAGGAACTCGGTTGTATATGAGCTATCGAAGCTGATGTCATATCTAAATTTAATGGTAAAAATTCCTATCAGTTCTCTTGGTTTCATACTCTACTCTAGTTTCCTTTAATTTGGCAAGCTTTTGGTATTAGCTGGTGGCAATGTTAAATTGTTGTACTTTGAATTTGACAATTTTATTTTGTAGGTAGACTTTTCAGAATTTTCACGCTTCTACGAGTTTGTTTTCTTTGTGTGTCGGGAGAATGGTCAAAAGAATATCAGTAAGATTCCTTCTTTAGACTTGGATATAAAAATATGTTTTAGTTTCCTAACACGAAGGATAACATGAAATATGCACTGGGTAAGTTAAAGTTTCTCTTACATTCTGATATTTTTTTTCTTCTTTATTTTATTTGAATTACAGCTGTTAGTATGGCAGTTAGAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGGTATCCTTTTCCCCCTTCTCTTTCTTTCCTTTTCTTTAGAACTTAAGGACACAGTGATACAACCTATAGAAAGATCTTGTCCAAATTAGGTATTTTCCTTTTTGTTAAGGTACCCTTTTTCCCCATTCTCTTTTGTGCTGCTACTTAAGTATAGTATTAATAATCAGGAAACCAGAATTTCTACCTGAAATCATTTTCATCAACTTGGGGTTATGGGGCCTAACAATTATGAAACATACGTACATGCTAAATACCCATGAATGAACATTTTAATGTAGTTGCTATCTGCAGACATTTGGATACACGTTCATATATATATTTTACAAAAATGCATGCATTTATGTTTGAATTAGATGAATTTGGAAAATTTTGGGTATTTTTGTACCTAAGCTAAATCTACCAGATTTCTTCCATCTGGGTTTGTGCATTCATGTTATTTTTTTGGTGTTTTATCCTTCAACTTGTTCCTTGAATTAGTAGTGCTATATAGTGCCACTTATCTCCAAGGATCTGATGTATTTAAGTTCCACAGAACAATCAGCGACACAATATTTCTGAGGATACTTGGCAGCAAGTCTTGGCTTTCAGCCGCTGTGTACATGAAAATCTGGAAGGGTATGATCCCGAAGGTATTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTGCAATGGAAGTAATTTATTAGATATTATTTGAATTAAGAATGTTAAGACATGGGAATTACGTCAAGTAATCCTTCTCCTTAACTGTTGAAGTTTTTTCGCTGATACAATTGAATTCTATCACAAGAAAAAGTATATTTTGTCAATTTATATAAGAAAAATATAATCCTACACTTTCATGTTTAACCTTCTGTTTTGGTTCTTCTATGGAGGCTTGAAATATGATGAGAATGAAAATATTTATTGATCTCATTATATTGTTATGGTGGCTTGGAATTGTTGTTTCTCAAGTGTATGCCTAATGCCTAAAAACATTTTTGAGGCTTGAGTTCCAAATAGTTATATGATGGGTGGAATAATACAATTAACAGCTGTCAGCATATTCTTCAGATAATGGAAGTCCATTTCTGAATGCATGGATATATAACACTGATTGTGTTAATATAGCCCTTAAGTACTTTTATCCTATGAAAAATACAGAAAACAAAAGTTAAAGAAGAGATTCATGGTCTTGCAGTGGATGGATACTTGATTTTTTTTTCGGTACTTTTATAATCATTGTGTTTAATACTGCATGGTTCTGTATTTTTCTCAAAATTTTCCAATTAACTAGAGGATTATCACTTCTCTTGAAGAAAGAGCCTCAAGACTAGCCCTTACTCTTTTGAAATGTTGTGTATGCTTTTAAATAAGCAGAATGCTTCTCTTTTCTTACTGTAATGTTTGGACTGACTCAGTTGCGTTCTTCTATCAGGTGCTTGGCCTGTTCTAATTGATGACTTCGTTGAGCACATGTACAGGTTCGTTTGTGGAGGTTCTTACAGTTGCAACAAGTTTGAAGTTTAATGTAGTTAAATAGTTCTGGGTTATATATCATTTTAAGTCCTCGTTATTATCATGGTTTAGCCTTGTACTAACACTTGGTCTGCTTATAATTATTGTGCAGGCGGTCAGGGTCTAATAAAGTCTCAAAGTTATGTTGTAATTGTGGAGACTCAGAATCACAGTCAGGTGTATTTGAAGATTCTCTTTCTGGTATAGAAAAAAAAATGTACCATTCTACATATGGATTTCCATGGCATGCAGAATGTTATATAAGCCGTCTTTTTCTGGTTGGTATTGTGTCAGGATTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGACGGCATTGTGTCTCTGAGTGATCCTAGAACCGGCTCCATGGAGTTAAGTCCTGTTACGAGCATCAAGAAAAGTAGATTCATGGCTTGCAGGCCAGTGAACTTGGAGATAAACTTACCATCATATACTGTAGATGAGAACATAGAAATGGTTCGTGCGGTAGGTTCCAAATCTCCATGTGCTGTTGAAGGTTGTCTGTCAAAAGGTTTTGCTGGGCTTTTCTCGACTCGTTTGTTCTTAGGATTGGACCAAGAAAGGAAAGCGTCATTCACGTGAGCACGAGCGACTTAGCCCTTATGATATCTACAGGTTGTATGATCTACAATGTGGTTATATCTTTCTTTTTTTCTTTTTGGTTGTGGTACATCATTTTTAATCAGACGAGTTGATGATTTGGCAGTAAACTAGAACAAAGGATTGAATATGTTACTAATGAGGGAAGAGAAATTAATGATTTGGCTTTACGTTGTAATTAGTCTGTTTCAATTCAAAGAACGAATAAGAATCTACATACTTTGTGTTTTA

mRNA sequence

ATGAACTCCAGTCTGATGTGCATCGCGTTAGGAACTAAAACGGCTAACTTGTTTGTAGATATTCGATCTCAAAATGGCTTTTGTCATGGGAATGAAGACTCTGGACATGTTGAAACACAAATGGCTAAAAGTTCAAAGCAGGCACTTGCAGAGCTCTTAATATATGTGCAATCATCACTGCAGATGGGGAACTCGGTTGTATATGAGCTATCGAAGCTGATGTCATATCTAAATTTAATGGTAGACTTTTCAGAATTTTCACGCTTCTACGAGTTTGTTTTCTTTGTGTGTCGGGAGAATGGTCAAAAGAATATCATAGTGCTATATAGTGCCACTTATCTCCAAGGATCTGATGTATTTAAGTTCCACAGAACAATCAGCGACACAATATTTCTGAGGATACTTGGCAGCAAGTCTTGGCTTTCAGCCGCTGTGTACATGAAAATCTGGAAGGGTGCTTGGCCTGTTCTAATTGATGACTTCGTTGAGCACATGTACAGGCGGTCAGGGTCTAATAAAGTCTCAAAGTTATGTTGTAATTGTGGAGACTCAGAATCACAGTCAGGTGTATTTGAAGATTCTCTTTCTGGATTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGACGGCATTGTGTCTCTGAGTGATCCTAGAACCGGCTCCATGGAGTTAAGTCCTGTTACGAGCATCAAGAAAAGTAGATTCATGGCTTGCAGGCCAGTGAACTTGGAGATAAACTTACCATCATATACTGTAGATGAGAACATAGAAATGGTTCGTGCGGTAGGTTCCAAATCTCCATGTGCTGTTGAAGGTTGTCTGTCAAAAGGTTTTGCTGGGCTTTTCTCGACTCGTTTGTTCTTAGGATTGGACCAAGAAAGGAAAGCGTCATTCACGTGAGCACGAGCGACTTAGCCCTTATGATATCTACAGGTTGTATGATCTACAATGTGGTTATATCTTTCTTTTTTTCTTTTTGGTTGTGGTACATCATTTTTAATCAGACGAGTTGATGATTTGGCAGTAAACTAGAACAAAGGATTGAATATGTTACTAATGAGGGAAGAGAAATTAATGATTTGGCTTTACGTTGTAATTAGTCTGTTTCAATTCAAAGAACGAATAAGAATCTACATACTTTGTGTTTTA

Coding sequence (CDS)

ATGAACTCCAGTCTGATGTGCATCGCGTTAGGAACTAAAACGGCTAACTTGTTTGTAGATATTCGATCTCAAAATGGCTTTTGTCATGGGAATGAAGACTCTGGACATGTTGAAACACAAATGGCTAAAAGTTCAAAGCAGGCACTTGCAGAGCTCTTAATATATGTGCAATCATCACTGCAGATGGGGAACTCGGTTGTATATGAGCTATCGAAGCTGATGTCATATCTAAATTTAATGGTAGACTTTTCAGAATTTTCACGCTTCTACGAGTTTGTTTTCTTTGTGTGTCGGGAGAATGGTCAAAAGAATATCATAGTGCTATATAGTGCCACTTATCTCCAAGGATCTGATGTATTTAAGTTCCACAGAACAATCAGCGACACAATATTTCTGAGGATACTTGGCAGCAAGTCTTGGCTTTCAGCCGCTGTGTACATGAAAATCTGGAAGGGTGCTTGGCCTGTTCTAATTGATGACTTCGTTGAGCACATGTACAGGCGGTCAGGGTCTAATAAAGTCTCAAAGTTATGTTGTAATTGTGGAGACTCAGAATCACAGTCAGGTGTATTTGAAGATTCTCTTTCTGGATTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGACGGCATTGTGTCTCTGAGTGATCCTAGAACCGGCTCCATGGAGTTAAGTCCTGTTACGAGCATCAAGAAAAGTAGATTCATGGCTTGCAGGCCAGTGAACTTGGAGATAAACTTACCATCATATACTGTAGATGAGAACATAGAAATGGTTCGTGCGGTAGGTTCCAAATCTCCATGTGCTGTTGAAGGTTGTCTGTCAAAAGGTTTTGCTGGGCTTTTCTCGACTCGTTTGTTCTTAGGATTGGACCAAGAAAGGAAAGCGTCATTCACGTGA

Protein sequence

MNSSLMCIALGTKTANLFVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNIIVLYSATYLQGSDVFKFHRTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVRAVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT

Homology

[Close]

BLAST of Lsi02G014800 vs. ExPASy Swiss-Prot
Match: Q9MBG8 (Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=AAR3 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 5.4e-34
Identity = 106/299 (35.45%), Postives = 149/299 (49.83%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           F  IRS    C+     G  E+Q    SK+A+ +LL  V++  Q  NS+  EL KLMS L
Sbjct: 19  FCAIRSGQQLCNKKPCDGE-ESQRFNLSKEAITQLLYLVENKFQARNSIFDELFKLMSRL 78

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIVLYSATYLQGSDVFKF--------------H 137
           +LMVDF+EF+ FY+FVFF+CRENGQKNI +  + T  +     +F               
Sbjct: 79  DLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNRWCDFIEKNQR 138

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  + ++L     +   +     +GAWPVLIDDFVEHMY   G NK + L C CGD
Sbjct: 139 HNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSILGPNKDTSLFCKCGD 198

Query: 198 SESQSGVFEDS---------LSGLKFFPGLKRKLP---EDMQMDGIVSLSDPRTGSMELS 257
           +ES+S ++++           +GL+  PGLKRK     ++ + D    + + +  S  L+
Sbjct: 199 TESESCLYQEDEHHKDYRRPHTGLRNIPGLKRKTSKKNDEEEEDEDEEVLETQNSSSLLN 258

Query: 258 PVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVRAVGSKSPCAVEGCLSKGFAGLFST 291
               IK S    C                         SKSPC++E  LS+GFA L ST
Sbjct: 259 -FKRIKTSNSPRC------------------------SSKSPCSIERSLSQGFASLLST 291

[Close]

BLAST of Lsi02G014800 vs. ExPASy TrEMBL
Match: A0A1S3CB35 (Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 5.0e-115
Identity = 228/305 (74.75%), Postives = 246/305 (80.66%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           + DIRSQNG+ HGNEDSG+ ETQM+KSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL
Sbjct: 22  YCDIRSQNGY-HGNEDSGYAETQMSKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 81

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIVLYS--ATYLQGSDVFKF------------H 137
           N MVDFSEFSRFYEF FFVCRENGQKNI V  +  A  L     F+              
Sbjct: 82  NFMVDFSEFSRFYEFXFFVCRENGQKNITVSMAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  + ++L     +   +     +GAWPVLIDDFVEHMYRRSGSNKVSKLCC CGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKVSKLCCTCGD 201

Query: 198 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMA 257
           SES+SGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVS SDPRTG+ME +PVTS+KKSRFMA
Sbjct: 202 SESESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDPRTGAMEFTPVTSVKKSRFMA 261

Query: 258 CRPVNLEINLPSYTVDENIEMVR---AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 306
           CRPVNLEINLPS T  ENIE+VR    VGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER
Sbjct: 262 CRPVNLEINLPSCTAAENIEVVRHSSTVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 321

[Close]

BLAST of Lsi02G014800 vs. ExPASy TrEMBL
Match: A0A0A0KU82 (Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G636560 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 3.9e-112
Identity = 227/305 (74.43%), Postives = 243/305 (79.67%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           + DIRSQNG+ HGNEDSG+ ETQM KSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL
Sbjct: 22  YCDIRSQNGY-HGNEDSGNAETQMCKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 81

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIVLYS--ATYLQGSDVFKF------------H 137
           N MVDFSEFSRFYEFVFFVCRENGQKNI V  +  A  L     F+              
Sbjct: 82  NFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  + ++L     +   +     +GAWPVLIDDFVEHMYRRSGSNKVSKL CNCGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKVSKLSCNCGD 201

Query: 198 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMA 257
           SE +SGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVS SDPRT +ME +PVTS+KKSRFMA
Sbjct: 202 SE-ESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDPRTSAMEFTPVTSVKKSRFMA 261

Query: 258 CRPVNLEINLPSYTVDENIEMVR---AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 306
           CRPVNLEIN PS T  ENIEMVR    VGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER
Sbjct: 262 CRPVNLEINSPSCTAAENIEMVRHNSTVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 321

[Close]

BLAST of Lsi02G014800 vs. ExPASy TrEMBL
Match: A0A6J1FFS5 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111445042 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.6e-105
Identity = 211/304 (69.41%), Postives = 237/304 (77.96%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           + DIRSQNG  HGNEDSGHVE+Q+AKSSKQALAEL +YV+SSLQMGNS+V EL+K+MS+ 
Sbjct: 22  YCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDELAKVMSHP 81

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIV--LYSATYLQGSDVFKF------------H 137
           NLMVDFSEFSRFYEFVFFV RENGQKNI V     A  L     F+              
Sbjct: 82  NLMVDFSEFSRFYEFVFFVSRENGQKNITVGRAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  +L++L     +   +     +GAWPVLIDDFVEHMYRRSGSNK+SKL C CGD
Sbjct: 142 HNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKISKLYCTCGD 201

Query: 198 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMA 257
           SESQSGVFEDSL GLK FPGLKRKLPEDMQ+D I+SLSDPRTGSMEL PVTS+KK+RFM 
Sbjct: 202 SESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDIMSLSDPRTGSMELIPVTSLKKTRFMG 261

Query: 258 CRPVNLEINLPSYTVDENIEMVR---AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 305
           CRPVNLE+NLPS T DENI+MVR   AVG KSPCA+EG LSKGFAGLFSTR  LGLD+ER
Sbjct: 262 CRPVNLEVNLPSCTADENIDMVRHNGAVGCKSPCAIEGFLSKGFAGLFSTRSILGLDRER 321

[Close]

BLAST of Lsi02G014800 vs. ExPASy TrEMBL
Match: A0A6J1JZZ8 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111489407 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 6.1e-105
Identity = 210/305 (68.85%), Postives = 234/305 (76.72%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           + DIRSQNG  HGNED GHVE+Q+AKSSKQALAEL +YV+SSLQMGNS+V EL+K+MS+ 
Sbjct: 73  YCDIRSQNGLYHGNEDCGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDELAKVMSHP 132

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIV--LYSATYLQGSDVFKF------------H 137
           NLMVDFSEFSRFYEFVFFV RENGQKNI V     A  L     F+              
Sbjct: 133 NLMVDFSEFSRFYEFVFFVSRENGQKNITVSRAVRAWRLVLDGRFRLLNQWCDFVENNQR 192

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  +L++L     +   +     +GAWPVLIDDFVEHMYRRSGSNK+ KL C CGD
Sbjct: 193 HNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKIPKLYCTCGD 252

Query: 198 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMA 257
           SESQSGVFEDSL GLK FPGLKRK PEDMQ+D I+S SDPRTGSMEL PVTS+KKSRFM 
Sbjct: 253 SESQSGVFEDSLPGLKSFPGLKRKFPEDMQVDDIMSPSDPRTGSMELIPVTSVKKSRFMG 312

Query: 258 CRPVNLEINLPSYTVDENIEMVR---AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 306
           CRPVNLE+NLPS T DENI+MVR   AVG KSPCA+EGCLSKGFAGLFST   LGLDQER
Sbjct: 313 CRPVNLEVNLPSCTADENIDMVRYNGAVGCKSPCAIEGCLSKGFAGLFSTCSILGLDQER 372

[Close]

BLAST of Lsi02G014800 vs. ExPASy TrEMBL
Match: A0A6J1BYJ7 (Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111006635 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 1.1e-101
Identity = 213/306 (69.61%), Postives = 233/306 (76.14%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           + DIRSQNGFC GNEDS HVETQ+AKSSK+ALA LL YVQSSLQMGNS+VYELSKLMSYL
Sbjct: 22  YCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYL 81

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIV--LYSATYLQGSDVFKF------------H 137
           NLMVDFSEFSRFYEFVFFVCRENGQKNI V     A  L     F+              
Sbjct: 82  NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQR 141

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  + ++L     +   +     +GAWPVLIDDFVEHMYRR  SN +S L CNCGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRR--SNTISNLQCNCGD 201

Query: 198 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMA 257
           +ESQS  FEDSL GLKFFPGLKRKLPEDMQMDG+VSL D RTG MELSPVTSIKKSR MA
Sbjct: 202 TESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMA 261

Query: 258 CRPVNLEINLPSYTVDENIEMVRAVG----SKSPCAVEGCLSKGFAGLFSTRLFLGLDQE 306
            +PVNLEINLP+ T DE++EMVR  G    +KSPCAVEGCLSKGFAGLFSTR FL LDQE
Sbjct: 262 GKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQE 321

[Close]

BLAST of Lsi02G014800 vs. NCBI nr
Match: XP_038890437.1 (uncharacterized protein LOC120080000 isoform X1 [Benincasa hispida])

HSP 1 Score: 438.7 bits (1127), Expect = 4.0e-119
Identity = 233/305 (76.39%), Postives = 249/305 (81.64%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           + DIRSQNGFCHGNE+SG VETQMAKS KQALAELLIYVQSSLQMGNS+VYELSKLMSYL
Sbjct: 22  YCDIRSQNGFCHGNEESGQVETQMAKSPKQALAELLIYVQSSLQMGNSIVYELSKLMSYL 81

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIVLYS--ATYLQGSDVFKF------------H 137
           N MVDFSEFSRFYEFVFFVCRENGQKNI V  +  A  L  +  F+              
Sbjct: 82  NFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRLVLNGRFRLLNQWCDFVENNQR 141

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  + ++L     +   +     +GAWPVLIDDFVEHMYR+SGSNKVSK CCNCGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKVSKFCCNCGD 201

Query: 198 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMA 257
           SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGI+S SDPRTGSMELSPV S+KKSRFMA
Sbjct: 202 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIMSPSDPRTGSMELSPVLSVKKSRFMA 261

Query: 258 CRPVNLEINLPSYTVDENIEMVR---AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 306
           CRPVNLEINLPS T DEN EMVR   AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER
Sbjct: 262 CRPVNLEINLPSCTADENTEMVRHNSAVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 321

[Close]

BLAST of Lsi02G014800 vs. NCBI nr
Match: XP_038890438.1 (uncharacterized protein LOC120080000 isoform X2 [Benincasa hispida])

HSP 1 Score: 435.6 bits (1119), Expect = 3.4e-118
Identity = 232/303 (76.57%), Postives = 248/303 (81.85%), Query Frame = 0


Query: 20  DIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYLNL 79
           +IRSQNGFCHGNE+SG VETQMAKS KQALAELLIYVQSSLQMGNS+VYELSKLMSYLN 
Sbjct: 6   NIRSQNGFCHGNEESGQVETQMAKSPKQALAELLIYVQSSLQMGNSIVYELSKLMSYLNF 65

Query: 80  MVDFSEFSRFYEFVFFVCRENGQKNIIVLYS--ATYLQGSDVFKF------------HRT 139
           MVDFSEFSRFYEFVFFVCRENGQKNI V  +  A  L  +  F+                
Sbjct: 66  MVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRLVLNGRFRLLNQWCDFVENNQRHN 125

Query: 140 ISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGDSE 199
           IS+  + ++L     +   +     +GAWPVLIDDFVEHMYR+SGSNKVSK CCNCGDSE
Sbjct: 126 ISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRQSGSNKVSKFCCNCGDSE 185

Query: 200 SQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMACR 259
           SQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGI+S SDPRTGSMELSPV S+KKSRFMACR
Sbjct: 186 SQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIMSPSDPRTGSMELSPVLSVKKSRFMACR 245

Query: 260 PVNLEINLPSYTVDENIEMVR---AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQERKA 306
           PVNLEINLPS T DEN EMVR   AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQERKA
Sbjct: 246 PVNLEINLPSCTADENTEMVRHNSAVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQERKA 305

[Close]

BLAST of Lsi02G014800 vs. NCBI nr
Match: XP_008459316.1 (PREDICTED: LOW QUALITY PROTEIN: defective in cullin neddylation protein 1 [Cucumis melo])

HSP 1 Score: 424.1 bits (1089), Expect = 1.0e-114
Identity = 228/305 (74.75%), Postives = 246/305 (80.66%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           + DIRSQNG+ HGNEDSG+ ETQM+KSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL
Sbjct: 22  YCDIRSQNGY-HGNEDSGYAETQMSKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 81

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIVLYS--ATYLQGSDVFKF------------H 137
           N MVDFSEFSRFYEF FFVCRENGQKNI V  +  A  L     F+              
Sbjct: 82  NFMVDFSEFSRFYEFXFFVCRENGQKNITVSMAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  + ++L     +   +     +GAWPVLIDDFVEHMYRRSGSNKVSKLCC CGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKVSKLCCTCGD 201

Query: 198 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMA 257
           SES+SGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVS SDPRTG+ME +PVTS+KKSRFMA
Sbjct: 202 SESESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDPRTGAMEFTPVTSVKKSRFMA 261

Query: 258 CRPVNLEINLPSYTVDENIEMVR---AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 306
           CRPVNLEINLPS T  ENIE+VR    VGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER
Sbjct: 262 CRPVNLEINLPSCTAAENIEVVRHSSTVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 321

[Close]

BLAST of Lsi02G014800 vs. NCBI nr
Match: XP_004148700.1 (uncharacterized protein LOC101214663 [Cucumis sativus] >KGN52464.1 hypothetical protein Csa_007882 [Cucumis sativus])

HSP 1 Score: 414.5 bits (1064), Expect = 8.1e-112
Identity = 227/305 (74.43%), Postives = 243/305 (79.67%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           + DIRSQNG+ HGNEDSG+ ETQM KSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL
Sbjct: 22  YCDIRSQNGY-HGNEDSGNAETQMCKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 81

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIVLYS--ATYLQGSDVFKF------------H 137
           N MVDFSEFSRFYEFVFFVCRENGQKNI V  +  A  L     F+              
Sbjct: 82  NFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  + ++L     +   +     +GAWPVLIDDFVEHMYRRSGSNKVSKL CNCGD
Sbjct: 142 HNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKVSKLSCNCGD 201

Query: 198 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMA 257
           SE +SGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVS SDPRT +ME +PVTS+KKSRFMA
Sbjct: 202 SE-ESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDPRTSAMEFTPVTSVKKSRFMA 261

Query: 258 CRPVNLEINLPSYTVDENIEMVR---AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 306
           CRPVNLEIN PS T  ENIEMVR    VGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER
Sbjct: 262 CRPVNLEINSPSCTAAENIEMVRHNSTVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 321

[Close]

BLAST of Lsi02G014800 vs. NCBI nr
Match: XP_023550121.1 (DCN1-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023550122.1 DCN1-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 395.6 bits (1015), Expect = 3.9e-106
Identity = 212/305 (69.51%), Postives = 237/305 (77.70%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           + DIRSQNG  HGNEDSGHVE+Q+AKSSKQALAEL +YV+SSLQMGNS+V EL+K+MS+ 
Sbjct: 22  YCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYVKSSLQMGNSIVDELAKVMSHP 81

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIV--LYSATYLQGSDVFKF------------H 137
           NLMVDFSEFSRFYEFVFFV RENGQKNI V     A  L     F+              
Sbjct: 82  NLMVDFSEFSRFYEFVFFVSRENGQKNITVSRAVRAWRLVLDGRFRLLNQWCDFVENNQR 141

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  +L++L     +   +     +GAWPVLIDDFVEHMYRRSGSNK+SKL C CGD
Sbjct: 142 HNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKISKLYCTCGD 201

Query: 198 SESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMA 257
           SESQSGVFEDSL GLK FPGLKRKLPEDMQ+DGI+SLSDPRTGSMEL PVTS+KKSRFM 
Sbjct: 202 SESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDGIMSLSDPRTGSMELIPVTSVKKSRFMG 261

Query: 258 CRPVNLEINLPSYTVDENIEMVR---AVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQER 306
           CRPVNLE+NLPS T D N +MVR   AVG K+PCA+EGCLSKGFAGLFST   LGLDQER
Sbjct: 262 CRPVNLEVNLPSCTADANTDMVRPNGAVGCKAPCAIEGCLSKGFAGLFSTCSVLGLDQER 321

[Close]

BLAST of Lsi02G014800 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 146.4 bits (368), Expect = 3.9e-35
Identity = 106/299 (35.45%), Postives = 149/299 (49.83%), Query Frame = 0


Query: 18  FVDIRSQNGFCHGNEDSGHVETQMAKSSKQALAELLIYVQSSLQMGNSVVYELSKLMSYL 77
           F  IRS    C+     G  E+Q    SK+A+ +LL  V++  Q  NS+  EL KLMS L
Sbjct: 19  FCAIRSGQQLCNKKPCDGE-ESQRFNLSKEAITQLLYLVENKFQARNSIFDELFKLMSRL 78

Query: 78  NLMVDFSEFSRFYEFVFFVCRENGQKNIIVLYSATYLQGSDVFKF--------------H 137
           +LMVDF+EF+ FY+FVFF+CRENGQKNI +  + T  +     +F               
Sbjct: 79  DLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFRLLNRWCDFIEKNQR 138

Query: 138 RTISDTIFLRILGSKSWLSAAVYMKIWKGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGD 197
             IS+  + ++L     +   +     +GAWPVLIDDFVEHMY   G NK + L C CGD
Sbjct: 139 HNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSILGPNKDTSLFCKCGD 198

Query: 198 SESQSGVFEDS---------LSGLKFFPGLKRKLP---EDMQMDGIVSLSDPRTGSMELS 257
           +ES+S ++++           +GL+  PGLKRK     ++ + D    + + +  S  L+
Sbjct: 199 TESESCLYQEDEHHKDYRRPHTGLRNIPGLKRKTSKKNDEEEEDEDEEVLETQNSSSLLN 258

Query: 258 PVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVRAVGSKSPCAVEGCLSKGFAGLFST 291
               IK S    C                         SKSPC++E  LS+GFA L ST
Sbjct: 259 -FKRIKTSNSPRC------------------------SSKSPCSIERSLSQGFASLLST 291

The following BLAST results are available for this feature:

BLAST of Lsi02G014800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG8	5.4e-34	35.45	Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Lsi02G014800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CB35	5.0e-115	74.75	Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 ...	[more]
A0A0A0KU82	3.9e-112	74.43	Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G6365...	[more]
A0A6J1FFS5	1.6e-105	69.41	Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1JZZ8	6.1e-105	68.85	Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]
A0A6J1BYJ7	1.1e-101	69.61	Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Lsi02G014800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890437.1	4.0e-119	76.39	uncharacterized protein LOC120080000 isoform X1 [Benincasa hispida]	[more]
XP_038890438.1	3.4e-118	76.57	uncharacterized protein LOC120080000 isoform X2 [Benincasa hispida]	[more]
XP_008459316.1	1.0e-114	74.75	PREDICTED: LOW QUALITY PROTEIN: defective in cullin neddylation protein 1 [Cucum...	[more]
XP_004148700.1	8.1e-112	74.43	uncharacterized protein LOC101214663 [Cucumis sativus] >KGN52464.1 hypothetical ...	[more]
XP_023550121.1	3.9e-106	69.51	DCN1-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023550122.1 DCN1-like prote...	[more]

BLAST of Lsi02G014800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28970.1	3.9e-35	35.45	Domain of unknown function (DUF298)	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014764	Defective-in-cullin neddylation protein	PANTHER	PTHR12281	RP42 RELATED	coord: 16..107 coord: 151..196
None	No IPR available	PANTHER	PTHR12281:SF31	DEFECTIVE IN CULLIN NEDDYLATION PROTEIN	coord: 16..107 coord: 151..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G014800.1	Lsi02G014800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051443	positive regulation of ubiquitin-protein transferase activity
biological_process	GO:0045116	protein neddylation
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0097602	cullin family protein binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0032182	ubiquitin-like protein binding