Lsi02G014760 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G014760
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Calcium-binding EF-hand family protein
Location	chr02: 20502961 .. 20503503 (-)
RNA-Seq Expression	Lsi02G014760
Synteny	Lsi02G014760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTTGGTCTCTACAACGTTTCAATCGACGACAAGACAACCACATCAAATCCCTCTTTCCGATTCCTCATTATGATCGACTTCCTCCGTTTTCAGAAACTTGCCTCTTGGCTCTACGCTCTCGCCTTCACAAAACGAAAACAGAGTAGTCTTCCGGCCACTTCAAAATCGGAGGAAGACGGCAGCGGGGAGACGGAGAGGCTGCTGAGTACAGAGGAGATGAAATGGGTTATGGGAAATTTAGGGATTGATTGCAGCGAAGAGTCGGTTCCTGTGTCTGGGTCAACGGAAGAAGTATCGAGGATGTTCGATGAAAGGGCGGCGTGTGCGGCGGAGATAAAAGAAGCTTTCGATGTGTTTGATGTGAATAGCGATGGGTTCATCGACGTTAAAGAGTTGCAGAGAGTGATGTGCGTTTTGGGGTTCAAAGAAGGAGAAGGGATTCAGAATTGCGAGAGGATGATTCAAAAATTTGATCAAAACAAAGATGGAAGAATTGATTTTCAAGAATTTCTTAAATTGATGGAGTCTGTGGTTTGA

mRNA sequence

ATGGATTTTGGTCTCTACAACGTTTCAATCGACGACAAGACAACCACATCAAATCCCTCTTTCCGATTCCTCATTATGATCGACTTCCTCCGTTTTCAGAAACTTGCCTCTTGGCTCTACGCTCTCGCCTTCACAAAACGAAAACAGAGTAGTCTTCCGGCCACTTCAAAATCGGAGGAAGACGGCAGCGGGGAGACGGAGAGGCTGCTGAGTACAGAGGAGATGAAATGGGTTATGGGAAATTTAGGGATTGATTGCAGCGAAGAGTCGGTTCCTGTGTCTGGGTCAACGGAAGAAGTATCGAGGATGTTCGATGAAAGGGCGGCGTGTGCGGCGGAGATAAAAGAAGCTTTCGATGTGTTTGATGTGAATAGCGATGGGTTCATCGACGTTAAAGAGTTGCAGAGAGTGATGTGCGTTTTGGGGTTCAAAGAAGGAGAAGGGATTCAGAATTGCGAGAGGATGATTCAAAAATTTGATCAAAACAAAGATGGAAGAATTGATTTTCAAGAATTTCTTAAATTGATGGAGTCTGTGGTTTGA

Coding sequence (CDS)

ATGGATTTTGGTCTCTACAACGTTTCAATCGACGACAAGACAACCACATCAAATCCCTCTTTCCGATTCCTCATTATGATCGACTTCCTCCGTTTTCAGAAACTTGCCTCTTGGCTCTACGCTCTCGCCTTCACAAAACGAAAACAGAGTAGTCTTCCGGCCACTTCAAAATCGGAGGAAGACGGCAGCGGGGAGACGGAGAGGCTGCTGAGTACAGAGGAGATGAAATGGGTTATGGGAAATTTAGGGATTGATTGCAGCGAAGAGTCGGTTCCTGTGTCTGGGTCAACGGAAGAAGTATCGAGGATGTTCGATGAAAGGGCGGCGTGTGCGGCGGAGATAAAAGAAGCTTTCGATGTGTTTGATGTGAATAGCGATGGGTTCATCGACGTTAAAGAGTTGCAGAGAGTGATGTGCGTTTTGGGGTTCAAAGAAGGAGAAGGGATTCAGAATTGCGAGAGGATGATTCAAAAATTTGATCAAAACAAAGATGGAAGAATTGATTTTCAAGAATTTCTTAAATTGATGGAGTCTGTGGTTTGA

Protein sequence

MDFGLYNVSIDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEEDGSGETERLLSTEEMKWVMGNLGIDCSEESVPVSGSTEEVSRMFDERAACAAEIKEAFDVFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESVV

Homology

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BLAST of Lsi02G014760 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.8e-24
Identity = 56/120 (46.67%), Postives = 82/120 (68.33%), Query Frame = 0


Query: 59  EEDGSGETERLLSTEEMKWVMGNLGIDCSEESVPVSGSTEEVSRMFDERAACAAEIKEAF 118
           ++D   + +  +S EE + VM +LG+  +++ +    S +EVS +F+E+ A   E+K+AF
Sbjct: 72  DDDDDDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAF 131

Query: 119 DVFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMES 178
           DVFD N DGFID  ELQRV+ +LGFK+G  + NC  MI+  D NKDG+IDF EF+K ME+
Sbjct: 132 DVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMET 191

[Close]

BLAST of Lsi02G014760 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.4e-23
Identity = 63/152 (41.45%), Postives = 90/152 (59.21%), Query Frame = 0


Query: 29  FLRFQKLASWLYALAFTKRKQSSLPATSKSEEDGSGETERLLSTEEMKWVMGNLGI--DC 88
           F RF  L      ++  K K      + K EE    + +  L  E++  VM +LG+  D 
Sbjct: 51  FSRFWPLVQHQQCVSEKKSKDLEFQTSIKHEEYRDDDDDG-LCREDVGMVMKSLGLSTDQ 110

Query: 89  SEESVPVSGSTEEVSRMFDERAACAAEIKEAFDVFDVNSDGFIDVKELQRVMCVLGFKEG 148
             E +    S++EVS +F+E+     E+K+AFDVFD N DGFID  +LQRV+ +LG K+G
Sbjct: 111 ENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQG 170

Query: 149 EGIQNCERMIQKFDQNKDGRIDFQEFLKLMES 179
             ++NC RMI+ FD +KDGRIDF  F+K ME+
Sbjct: 171 SNLENCRRMIRSFDGSKDGRIDFYGFVKFMEN 201

[Close]

BLAST of Lsi02G014760 vs. ExPASy Swiss-Prot
Match: Q09011 (Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-12
Identity = 36/69 (52.17%), Postives = 46/69 (66.67%), Query Frame = 0


Query: 112 AEIKEAFDVFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQE 171
           +++KEAFDVFD N DGFI  KELQ V+  LG  EG  I   E MI   +Q+ DGR+DF E
Sbjct: 128 SDLKEAFDVFDENGDGFISAKELQVVLEKLGLPEGSEIDRVEMMISSVEQDHDGRVDFFE 187

Query: 172 FLKLMESVV 181
           F  +M +V+
Sbjct: 188 FKDMMRTVI 196

[Close]

BLAST of Lsi02G014760 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-12
Identity = 36/87 (41.38%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 90  SVPVSGSTEEVSRMFDERAACAAEIKEAFDVFDVNSDGFIDVKELQRVMCVLGFKEGEGI 149
           S+   G   +  R  +E      +++EAF VFD N DG+I V EL  V+  LG K+G   
Sbjct: 155 SIMAGGDDSKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQGRTA 214

Query: 150 QNCERMIQKFDQNKDGRIDFQEFLKLM 177
           + C RMI + D++ DGR+DF EFL++M
Sbjct: 215 EECRRMIGQVDRDGDGRVDFHEFLQMM 241

[Close]

BLAST of Lsi02G014760 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-12
Identity = 46/136 (33.82%), Postives = 76/136 (55.88%), Query Frame = 0


Query: 59  EEDGSGETERLLSTEEMKWVMGNLGIDCSE-------ESVPVSG----STEEVSRMF--- 118
           ++DG G+    ++T+E+     NLGI   E       + + V+G      EE   ++   
Sbjct: 14  DKDGDGK----ITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTI 73

Query: 119 ---DERAACAAEIKEAFDVFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQ 178
              DE      ++KEAF+VFD N DGFI V EL+ V+  LG K+G+ ++ C +MI + D 
Sbjct: 74  MVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDV 133

[Close]

BLAST of Lsi02G014760 vs. ExPASy TrEMBL
Match: A0A1S3CAB3 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.2e-71
Identity = 139/181 (76.80%), Postives = 161/181 (88.95%), Query Frame = 0


Query: 1   MDFGLYNVSIDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEE 60
           M+FGLYNVS+DDK+T  NPSFRFLI+IDFLRFQ LASWL + A TK KQS+LPA+SKS E
Sbjct: 1   MNFGLYNVSMDDKSTPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASSKSVE 60

Query: 61  DGSGETERLLSTEEMKWVMGNLGIDCSE-ESVPVSGSTEEVSRMFDERAACAAEIKEAFD 120
             +GE ERL+STEEMKWVMGNLG+DCSE  SVP+ GS EEV+ MFD+ AA AAE+K+AFD
Sbjct: 61  SSAGERERLVSTEEMKWVMGNLGMDCSEGSSVPLFGSAEEVAEMFDQTAASAAEVKQAFD 120

Query: 121 VFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESV 180
           VFDVN+DGFIDV+ELQRVMCVLGFKEGEGI+NCE+MI KFD NKDGRIDF+EF+KLME+V
Sbjct: 121 VFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVKLMEAV 180

[Close]

BLAST of Lsi02G014760 vs. ExPASy TrEMBL
Match: A0A5A7T9C9 (Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002800 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.5e-66
Identity = 131/172 (76.16%), Postives = 151/172 (87.79%), Query Frame = 0


Query: 10  IDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEEDGSGETERL 69
           +DDK+T  NPSFRFLI+IDFLRFQ LASWL + A TK KQS+LPA+SKS E  +GE ERL
Sbjct: 1   MDDKSTPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASSKSVESSAGERERL 60

Query: 70  LSTEEMKWVMGNLGIDCSE-ESVPVSGSTEEVSRMFDERAACAAEIKEAFDVFDVNSDGF 129
           +STEEMKWVMGNLG+DCSE  SVP+ GS EEV+ MFD+ AA AAE+K+AFDVFDVN DGF
Sbjct: 61  VSTEEMKWVMGNLGMDCSEGSSVPLFGSAEEVAEMFDQTAASAAEVKQAFDVFDVNGDGF 120

Query: 130 IDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESVV 181
           IDV+ELQRVMCVLGFKEGEGI+NCE+MI KFD NKDGRIDF+EF+KLME+VV
Sbjct: 121 IDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVKLMEAVV 172

[Close]

BLAST of Lsi02G014760 vs. ExPASy TrEMBL
Match: A0A0A0KS94 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.3e-65
Identity = 133/181 (73.48%), Postives = 154/181 (85.08%), Query Frame = 0


Query: 1   MDFGLYNVSIDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEE 60
           M+ GLYNVS+DDK+T  NPSFRFL++IDFLRFQ LASWL +   TK KQS+ PA+SKS E
Sbjct: 2   MNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVE 61

Query: 61  DGSGETERLLSTEEMKWVMGNLGIDCSE-ESVPVSGSTEEVSRMFDERAACAAEIKEAFD 120
             S   ERL+STEEMKWVMGNLGIDCSE  SVP+ GS EEV+ MFD+ AA  AE+K+AF 
Sbjct: 62  --SRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFG 121

Query: 121 VFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESV 180
           VFDVN DGFIDV+ELQRVMCVLGFKEGEGI+NCE+MI+KFD NKDGRIDF+EF+KLME+V
Sbjct: 122 VFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAV 180

[Close]

BLAST of Lsi02G014760 vs. ExPASy TrEMBL
Match: A0A6J1JZ70 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111489598 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.4e-53
Identity = 114/174 (65.52%), Postives = 134/174 (77.01%), Query Frame = 0


Query: 10  IDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEEDGSGETERL 69
           +D  T  +NPSFRFLIMID  RFQKL SWLYA A  KRKQ SLPA+  ++ +   E    
Sbjct: 1   MDGNTIPANPSFRFLIMIDCFRFQKLGSWLYAFASGKRKQWSLPASETAKSEQRKEGGER 60

Query: 70  LSTEEMKWVMGNLGI---DCSEESVPVSGSTEEVSRMFDERAACAAEIKEAFDVFDVNSD 129
           +S EEMKWVM  LG+   + +EES+P   S EE+ ++FD+ AA AAE+KEAFDVFDVNSD
Sbjct: 61  VSREEMKWVMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSD 120

Query: 130 GFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESVV 181
           GFIDV+ELQRV+CVLGFKEGE IQNC RMI KFD+N DGRIDFQEF+KLMES V
Sbjct: 121 GFIDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Lsi02G014760 vs. ExPASy TrEMBL
Match: A0A6J1FKT4 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111445127 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 9.3e-53
Identity = 114/174 (65.52%), Postives = 131/174 (75.29%), Query Frame = 0


Query: 10  IDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEEDGSGETERL 69
           +D  T  + PSFRFLIMID  RFQKL SWLYA A  KRKQ SLPA+  ++ +   E    
Sbjct: 1   MDGNTIPAIPSFRFLIMIDCFRFQKLGSWLYAFATGKRKQWSLPASETAKSEQRDEGGER 60

Query: 70  LSTEEMKWVMGNLGIDC---SEESVPVSGSTEEVSRMFDERAACAAEIKEAFDVFDVNSD 129
           +S EEMKWVMG LG+     +EES+P   S EE+ R+FD+ AA AAE+KEAFDVFDVNSD
Sbjct: 61  VSREEMKWVMGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSD 120

Query: 130 GFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESVV 181
           GFID  ELQRV+CVLGFKEGE IQNC RMI KFD+N DGRIDFQEF+KLMES V
Sbjct: 121 GFIDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Lsi02G014760 vs. NCBI nr
Match: XP_038889669.1 (probable calcium-binding protein CML46 [Benincasa hispida])

HSP 1 Score: 289.3 bits (739), Expect = 2.3e-74
Identity = 147/180 (81.67%), Postives = 161/180 (89.44%), Query Frame = 0


Query: 1   MDFGLYNVSIDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEE 60
           MDFG+YNVS+DDKTTT NPSFRFLI+IDFLRFQKLASWLYA A TK+K SS PATSK+EE
Sbjct: 1   MDFGVYNVSMDDKTTTQNPSFRFLIVIDFLRFQKLASWLYAFATTKQKPSSFPATSKTEE 60

Query: 61  DGSGETERLLSTEEMKWVMGNLGIDCSEESVPVSGSTEEVSRMFDERAACAAEIKEAFDV 120
            G GE ERL++TEEMKWVMGNLGIDC EESVP+ GSTEEV+ MFDERAA   E+K+AFDV
Sbjct: 61  HG-GE-ERLMTTEEMKWVMGNLGIDCGEESVPLFGSTEEVAEMFDERAASTVEVKKAFDV 120

Query: 121 FDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESVV 180
           FDVN DGFID +ELQRVMCVLGFKEGE IQNCERMI+KFD N DGRIDF+EFLKLMESVV
Sbjct: 121 FDVNGDGFIDSEELQRVMCVLGFKEGERIQNCERMIRKFDHNGDGRIDFEEFLKLMESVV 178

[Close]

BLAST of Lsi02G014760 vs. NCBI nr
Match: XP_008459700.2 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 279.3 bits (713), Expect = 2.4e-71
Identity = 139/181 (76.80%), Postives = 161/181 (88.95%), Query Frame = 0


Query: 1   MDFGLYNVSIDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEE 60
           M+FGLYNVS+DDK+T  NPSFRFLI+IDFLRFQ LASWL + A TK KQS+LPA+SKS E
Sbjct: 1   MNFGLYNVSMDDKSTPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASSKSVE 60

Query: 61  DGSGETERLLSTEEMKWVMGNLGIDCSE-ESVPVSGSTEEVSRMFDERAACAAEIKEAFD 120
             +GE ERL+STEEMKWVMGNLG+DCSE  SVP+ GS EEV+ MFD+ AA AAE+K+AFD
Sbjct: 61  SSAGERERLVSTEEMKWVMGNLGMDCSEGSSVPLFGSAEEVAEMFDQTAASAAEVKQAFD 120

Query: 121 VFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESV 180
           VFDVN+DGFIDV+ELQRVMCVLGFKEGEGI+NCE+MI KFD NKDGRIDF+EF+KLME+V
Sbjct: 121 VFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVKLMEAV 180

[Close]

BLAST of Lsi02G014760 vs. NCBI nr
Match: KAA0039468.1 (putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYK00658.1 putative calcium-binding protein CML30 [Cucumis melo var. makuwa])

HSP 1 Score: 262.3 bits (669), Expect = 3.1e-66
Identity = 131/172 (76.16%), Postives = 151/172 (87.79%), Query Frame = 0


Query: 10  IDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEEDGSGETERL 69
           +DDK+T  NPSFRFLI+IDFLRFQ LASWL + A TK KQS+LPA+SKS E  +GE ERL
Sbjct: 1   MDDKSTPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASSKSVESSAGERERL 60

Query: 70  LSTEEMKWVMGNLGIDCSE-ESVPVSGSTEEVSRMFDERAACAAEIKEAFDVFDVNSDGF 129
           +STEEMKWVMGNLG+DCSE  SVP+ GS EEV+ MFD+ AA AAE+K+AFDVFDVN DGF
Sbjct: 61  VSTEEMKWVMGNLGMDCSEGSSVPLFGSAEEVAEMFDQTAASAAEVKQAFDVFDVNGDGF 120

Query: 130 IDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESVV 181
           IDV+ELQRVMCVLGFKEGEGI+NCE+MI KFD NKDGRIDF+EF+KLME+VV
Sbjct: 121 IDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVKLMEAVV 172

[Close]

BLAST of Lsi02G014760 vs. NCBI nr
Match: XP_031741770.1 (probable calcium-binding protein CML47 [Cucumis sativus])

HSP 1 Score: 259.2 bits (661), Expect = 2.6e-65
Identity = 133/181 (73.48%), Postives = 154/181 (85.08%), Query Frame = 0


Query: 1   MDFGLYNVSIDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEE 60
           M+ GLYNVS+DDK+T  NPSFRFL++IDFLRFQ LASWL +   TK KQS+ PA+SKS E
Sbjct: 132 MNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVE 191

Query: 61  DGSGETERLLSTEEMKWVMGNLGIDCSE-ESVPVSGSTEEVSRMFDERAACAAEIKEAFD 120
             S   ERL+STEEMKWVMGNLGIDCSE  SVP+ GS EEV+ MFD+ AA  AE+K+AF 
Sbjct: 192 --SRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFG 251

Query: 121 VFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESV 180
           VFDVN DGFIDV+ELQRVMCVLGFKEGEGI+NCE+MI+KFD NKDGRIDF+EF+KLME+V
Sbjct: 252 VFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAV 310

[Close]

BLAST of Lsi02G014760 vs. NCBI nr
Match: KAG6578574.1 (putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 219.5 bits (558), Expect = 2.3e-53
Identity = 115/174 (66.09%), Postives = 131/174 (75.29%), Query Frame = 0


Query: 10  IDDKTTTSNPSFRFLIMIDFLRFQKLASWLYALAFTKRKQSSLPATSKSEEDGSGETERL 69
           +D  T  +NPSFRFLIMID  RFQKL SWLYA A  KRKQ SLPA+   + +   E    
Sbjct: 1   MDGNTIPANPSFRFLIMIDCFRFQKLGSWLYAFATGKRKQWSLPASETGKSEQRDEGGER 60

Query: 70  LSTEEMKWVMGNLGIDC---SEESVPVSGSTEEVSRMFDERAACAAEIKEAFDVFDVNSD 129
           +S EEMKWVMG LG+     +EES+P   S EE+ R+FD+ AA AAE+KEAFDVFDVNSD
Sbjct: 61  VSREEMKWVMGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSD 120

Query: 130 GFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMESVV 181
           GFID  ELQRV+CVLGFKEGE IQNC RMI KFD+N DGRIDFQEF+KLMES V
Sbjct: 121 GFIDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Lsi02G014760 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 114.0 bits (284), Expect = 1.3e-25
Identity = 56/120 (46.67%), Postives = 82/120 (68.33%), Query Frame = 0


Query: 59  EEDGSGETERLLSTEEMKWVMGNLGIDCSEESVPVSGSTEEVSRMFDERAACAAEIKEAF 118
           ++D   + +  +S EE + VM +LG+  +++ +    S +EVS +F+E+ A   E+K+AF
Sbjct: 72  DDDDDDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAF 131

Query: 119 DVFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEFLKLMES 178
           DVFD N DGFID  ELQRV+ +LGFK+G  + NC  MI+  D NKDG+IDF EF+K ME+
Sbjct: 132 DVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMET 191

[Close]

BLAST of Lsi02G014760 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 108.6 bits (270), Expect = 5.3e-24
Identity = 63/152 (41.45%), Postives = 90/152 (59.21%), Query Frame = 0


Query: 29  FLRFQKLASWLYALAFTKRKQSSLPATSKSEEDGSGETERLLSTEEMKWVMGNLGI--DC 88
           F RF  L      ++  K K      + K EE    + +  L  E++  VM +LG+  D 
Sbjct: 51  FSRFWPLVQHQQCVSEKKSKDLEFQTSIKHEEYRDDDDDG-LCREDVGMVMKSLGLSTDQ 110

Query: 89  SEESVPVSGSTEEVSRMFDERAACAAEIKEAFDVFDVNSDGFIDVKELQRVMCVLGFKEG 148
             E +    S++EVS +F+E+     E+K+AFDVFD N DGFID  +LQRV+ +LG K+G
Sbjct: 111 ENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQG 170

Query: 149 EGIQNCERMIQKFDQNKDGRIDFQEFLKLMES 179
             ++NC RMI+ FD +KDGRIDF  F+K ME+
Sbjct: 171 SNLENCRRMIRSFDGSKDGRIDFYGFVKFMEN 201

[Close]

BLAST of Lsi02G014760 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 73.9 bits (180), Expect = 1.4e-13
Identity = 46/136 (33.82%), Postives = 76/136 (55.88%), Query Frame = 0


Query: 59  EEDGSGETERLLSTEEMKWVMGNLGIDCSE-------ESVPVSG----STEEVSRMF--- 118
           ++DG G+    ++T+E+     NLGI   E       + + V+G      EE   ++   
Sbjct: 14  DKDGDGK----ITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTI 73

Query: 119 ---DERAACAAEIKEAFDVFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQ 178
              DE      ++KEAF+VFD N DGFI V EL+ V+  LG K+G+ ++ C +MI + D 
Sbjct: 74  MVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDV 133

[Close]

BLAST of Lsi02G014760 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 72.8 bits (177), Expect = 3.2e-13
Identity = 33/75 (44.00%), Postives = 54/75 (72.00%), Query Frame = 0


Query: 103 MFDERAACAAEIKEAFDVFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQN 162
           + DE      ++KEAF+VFD N DGFI V EL+ V+  LG K+G+ + +C++MI+K D +
Sbjct: 69  IMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVD 128

Query: 163 KDGRIDFQEFLKLME 178
            DGR++++EF ++M+
Sbjct: 129 GDGRVNYKEFRQMMK 143

[Close]

BLAST of Lsi02G014760 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 71.6 bits (174), Expect = 7.1e-13
Identity = 30/65 (46.15%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 113 EIKEAFDVFDVNSDGFIDVKELQRVMCVLGFKEGEGIQNCERMIQKFDQNKDGRIDFQEF 172
           ++K+AF+VFD + DGFI V+EL+ VM  LG K+G+ +  C++MI + D + DGR++++EF
Sbjct: 143 DMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEF 202

Query: 173 LKLME 178
           L++M+
Sbjct: 203 LQMMK 207

The following BLAST results are available for this feature:

BLAST of Lsi02G014760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	1.8e-24	46.67	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	7.4e-23	41.45	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q09011	2.0e-12	52.17	Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q0JC44	2.0e-12	41.38	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9ZR02	2.0e-12	33.82	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]

BLAST of Lsi02G014760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CAB3	1.2e-71	76.80	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 P...	[more]
A0A5A7T9C9	1.5e-66	76.16	Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0KS94	1.3e-65	73.48	Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1	[more]
A0A6J1JZ70	2.4e-53	65.52	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114895...	[more]
A0A6J1FKT4	9.3e-53	65.52	probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of Lsi02G014760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889669.1	2.3e-74	81.67	probable calcium-binding protein CML46 [Benincasa hispida]	[more]
XP_008459700.2	2.4e-71	76.80	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
KAA0039468.1	3.1e-66	76.16	putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYK00658.1 pu...	[more]
XP_031741770.1	2.6e-65	73.48	probable calcium-binding protein CML47 [Cucumis sativus]	[more]
KAG6578574.1	2.3e-53	66.09	putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Lsi02G014760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	1.3e-25	46.67	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	5.3e-24	41.45	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	1.4e-13	33.82	EF hand calcium-binding protein family	[more]
AT1G05990.1	3.2e-13	44.00	EF hand calcium-binding protein family	[more]
AT2G43290.1	7.1e-13	46.15	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 151..179 e-value: 3.9E-6 score: 36.4 coord: 113..141 e-value: 9.8E-5 score: 31.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 113..177 e-value: 6.5E-14 score: 52.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 109..144 score: 14.597092
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 147..180 score: 15.071314
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 113..176 e-value: 1.15998E-17 score: 71.0397
None	No IPR available	GENE3D	1.10.238.10		coord: 110..178 e-value: 3.4E-21 score: 77.2
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 13..178
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 13..178
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 122..134
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 160..172
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 69..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G014760.1	Lsi02G014760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding