Lsi02G014560 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G014560 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.6e-131
Identity = 245/531 (46.14%), Postives = 338/531 (63.65%), Query Frame = 0

Query: 13  SLLLLSFLIW-----DCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSS--SSYSYVLNFT 72
           S+L+L F ++       L    D  ++CV  +   Y       + P+   S +S VL  T
Sbjct: 11  SILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLEST 70

Query: 73  IRNLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANI-- 132
            +NLRF   ++PKP  I  P   S VQ ++IC KK  +H+R+RSGGHD+EGLSYV+ I  
Sbjct: 71  AQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDK 130

Query: 133 PFLLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFL 192
           PF+L+DL+ +  V +   DN+AWVQSGA++GELYY++ +++   GFP G+  ++G+GG +
Sbjct: 131 PFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHI 190

Query: 193 SGGGYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWK 252
           +GG YG M+RKYGL  DNV+DA++VDANG+LLDR  MGED FWAIRGG GGSFGI+LAWK
Sbjct: 191 TGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWK 250

Query: 253 LKLVPVPPIVTSFTTFKSWDQN-STKLVHRWQYVAPEIDERLFIGIMVT----------- 312
           +KLVPVP  VT FT  K+  Q+   K++ +WQ VA ++ E LFI ++             
Sbjct: 251 IKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVT 310

Query: 313 --------GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLID- 372
                   G    L+++M KSFPELGL  +DC+E +W+ES+AY  SGF T    N L+  
Sbjct: 311 TSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAY-ISGFPTHTPTNVLLQG 370

Query: 373 RTPLSNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNT 432
           ++P     +K KSD+   PI E+  QGI+++       +   +I  PYGG M +  E+  
Sbjct: 371 KSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKE---DIPLMIWNPYGGMMAKIPESQI 430

Query: 433 PSPHRAGYPIKIAYYVTW-NSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIG 492
           P PHR G   K+ Y  +W +S +  SRH+ W R+LY YMTP+VS +PR AYVNYRDLD+G
Sbjct: 431 PFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLG 490

Query: 493 RNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPV 512
           RN +D + T  ++A VWG  YF +NF RL+ +K KVDP N FRHEQSIPP+
Sbjct: 491 RNTKD-VKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Lsi02G014560 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 2.0e-129
Identity = 233/530 (43.96%), Postives = 326/530 (61.51%), Query Frame = 0

Query: 12  VSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRNLRF 71
           +    LSF I   + +  +N L C S H     +N  L+Y      Y  +LN TI+NLRF
Sbjct: 14  IIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRF 73

Query: 72  SGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPFLLIDLA 131
                PKP+ I+ PS  S +Q T++C KK  + IR RSGGHD EG+SY++ +PF+++DL 
Sbjct: 74  ISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLR 133

Query: 132 NLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGYGLM 191
           N++++ ++    TAWV++GA++GE+YY + ++  +  FPGG  P VGVGG  SGGGYG +
Sbjct: 134 NMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGAL 193

Query: 192 VRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPP 251
           +R YGLA DN++DA LV+ +G++LDRK MGEDLFWAIRGGGG +FGI+ AWK+KLV VP 
Sbjct: 194 MRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPS 253

Query: 252 IVTSFTTFKSWD-QNSTKLVHRWQYVAPEIDERL-----FIGIMVT-------------- 311
             T F+  K+ +     KL ++WQ +A + D+ L     FI   +T              
Sbjct: 254 KSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYF 313

Query: 312 -----GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNF----LID 371
                G  D L+ +MNKSFPELG+KK DC E +W+++  +  SG V     NF    L+D
Sbjct: 314 SSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIF-YSGVVNFNTANFKKEILLD 373

Query: 372 RTPLSNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNT 431
           R+      +  K DY  +PI ETA   I E+    + +     +L PYGG M E SE+  
Sbjct: 374 RSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYE-EDVGAGMYVLYPYGGIMEEISESAI 433

Query: 432 PSPHRAGYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGR 491
           P PHRAG   ++ Y  +W   E + +H+ W R +Y + TP+VS++PR AY+NYRDLD+G+
Sbjct: 434 PFPHRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGK 493

Query: 492 NNEDGIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPV 512
            N    P +Y +A +WG KYFG NF RLV+VK KVDP N FR+EQSIPP+
Sbjct: 494 TNH-ASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Lsi02G014560 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 2.6e-129
Identity = 231/500 (46.20%), Postives = 329/500 (65.80%), Query Frame = 0

Query: 28  NLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRNLRFSGPNIPKPIAIIKPSQ 87
           N  + L C+S   +  +  S +I+ P+ +S+S VL  +I+N RFS P++PKP+ I+ P Q
Sbjct: 33  NQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQ 92

Query: 88  PSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPFLLIDLANLNAVTVEDDN-TAWV 147
           PS VQ+ V C ++  IHIR RSGGHD+EGLSYV + PF+++DL NL ++TV+ DN + WV
Sbjct: 93  PSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWV 152

Query: 148 QSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNVVDAQL 207
           Q+GA++GELYY++G++  +  FP GV P VGVGG  SGGGYG ++RK+GLA D+V+DA++
Sbjct: 153 QTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARV 212

Query: 208 VDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFTTFKSWDQNST 267
           VDA G +L+R+ MGED FWAIRGGGG SF +VL+WK+ L+ VP  VT F   K  +Q++ 
Sbjct: 213 VDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSAL 272

Query: 268 KLVHRWQYVAPEIDERLFIGIMV---------------TGRADELISIMNKSFPELGLKK 327
           K++HRWQ+VA ++ + LFI +M+                G    L+ ++NK FPELGL++
Sbjct: 273 KIIHRWQFVADKVSDDLFIRVMLQRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEE 332

Query: 328 EDCVESNWVESMAYPASGFVTTKDMNFLIDRTPLSNGRYKTKSDYATEPISETAFQGIWE 387
           +DC E +W+ES+ + A   +  + +N L  RT  S   +K KSD+  EP+ +TA   +W 
Sbjct: 333 DDCTEMSWIESVIWFAE--LGEEPINVLTKRTRASLA-FKAKSDFVQEPMPKTAISKLWR 392

Query: 388 RFNNAKGIETVQLILIPYGGKMNEFSETNTPSPHRAGYPIKIAYYVTWNSPEGDSRHLEW 447
           R    +  E  QLI  P+GGKM+E ++  TP PHR G   +I Y   W   +   +++ W
Sbjct: 393 RLQEPEA-EHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG-DVKEKYMRW 452

Query: 448 TRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNEDGIPTS-YEEASVWGLKYFGDNFERLV 507
              +Y+ M+ FV+KSPR AY+N RDLD+G     G+  S YEE   WG+KYF +NFERLV
Sbjct: 453 VERVYDDMSEFVAKSPRGAYINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKNNFERLV 512

Query: 508 EVKRKVDPFNVFRHEQSIPP 511
            VK  VDP + F  EQSIPP
Sbjct: 513 RVKTSVDPSDFFCDEQSIPP 525

BLAST of Lsi02G014560 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 4.5e-129
Identity = 235/523 (44.93%), Postives = 330/523 (63.10%), Query Frame = 0

Query: 8   YFSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIR 67
           YF  ++L L  F        N +N L C+S   ++ DS   +I+     SY  +LN +I+
Sbjct: 13  YFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDSR--IIHTSKDPSYFSILNSSIQ 72

Query: 68  NLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVA-NIPFL 127
           N RF     PKP++II P Q + VQ+T+ C + H IHIR RSGGHD+EGLSY+A + PF+
Sbjct: 73  NPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFV 132

Query: 128 LIDLANLNAVTVEDDN-TAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGG 187
           +IDL NL ++T++ DN T WVQSGA++GELYY++G+ + S  FP G+ P VG+GG   GG
Sbjct: 133 VIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGG 192

Query: 188 GYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKL 247
           GYG ++RKYGL+ DNV+DA +VDANG  LDR+ MGED FWAIRGGGG SF +VL+WK++L
Sbjct: 193 GYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRL 252

Query: 248 VPVPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMV--------------TG 307
           + VP +VT F   K+ ++ +  ++++WQY+A ++   LFI  M+               G
Sbjct: 253 LDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETEVYASFPGLYLG 312

Query: 308 RADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDRTPLSNGRYK 367
              +L+++M   FPELGL+  +C E +W+ES+ +    F+  + M  L  R   S   +K
Sbjct: 313 PVSDLLALMKDKFPELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKRTSRS-FK 372

Query: 368 TKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPSPHRAGYPI 427
            K D+  EPI +TA Q +W RF  A      ++IL P+GGKM+E ++   P PHR G   
Sbjct: 373 GKDDFIEEPIPKTAIQYLWRRF-EAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLY 432

Query: 428 KIAYYVTWNSPE-----GDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNEDG 487
           +I Y   W+  E        ++L W   +YE+MTP+VSKSPR AYVN+RD+D+G      
Sbjct: 433 EIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLN 492

Query: 488 IPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIP 510
           + T YEEA VWG+KYF +NF+RLV VK  VDP + F  EQSIP
Sbjct: 493 MKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of Lsi02G014560 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 9.9e-129
Identity = 232/530 (43.77%), Postives = 322/530 (60.75%), Query Frame = 0

Query: 12  VSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRNLRF 71
           +    LSF I   + +  +N L C S +     +N   IY      Y  VLN TI+NLRF
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRF 73

Query: 72  SGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPFLLIDLA 131
           +    PKP+ I+ PS  S +Q +++C KK  + IR RSGGHD EGLSY++ +PF ++DL 
Sbjct: 74  TSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLR 133

Query: 132 NLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGYGLM 191
           N++ V V+    TAWV++GA++GE+YY + +   +F FPGG  P VGVGG  SGGGYG +
Sbjct: 134 NMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGAL 193

Query: 192 VRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPP 251
           +R YGLA DN++DA LV+ +G++LDRK MGEDLFWAIRGGGG +FGI+ AWK+KLV VP 
Sbjct: 194 MRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPS 253

Query: 252 IVTSFTTFKSWD-QNSTKLVHRWQYVAPEIDERL------------------------FI 311
             T F+  K+ +     KL ++WQ +A + D+ L                        + 
Sbjct: 254 KATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYF 313

Query: 312 GIMVTGRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNF----LID 371
             +  G  D L+ +MNKSFPELG+KK DC E +W+++  +  SG V     NF    L+D
Sbjct: 314 SSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIF-YSGVVNYNTANFKKEILLD 373

Query: 372 RTPLSNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNT 431
           R+      +  K DY  + I ETA   I E+    + +     +L PYGG M+E SE+  
Sbjct: 374 RSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEE-VGVGMYVLYPYGGIMDEISESAI 433

Query: 432 PSPHRAGYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGR 491
           P PHRAG   ++ Y  TW   E + +H+ W R +Y + TP+VS++PR AY+NYRDLD+G+
Sbjct: 434 PFPHRAGIMYELWYTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGK 493

Query: 492 NNEDGIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPV 512
            N +  P +Y +A +WG KYFG NF RLV+VK K DP N FR+EQSIPP+
Sbjct: 494 TNPES-PNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of Lsi02G014560 vs. ExPASy TrEMBL
Match: A0A6J1HLR7 (berberine bridge enzyme-like 26 OS=Cucurbita moschata OX=3662 GN=LOC111464766 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.5e-236
Identity = 410/529 (77.50%), Postives = 451/529 (85.26%), Query Frame = 0

Query: 7   NYFSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTI 66
           NY + V+LLL  F I D  G NLD+ L CV    S  DSN  LI  PSSS+YS VLNFT+
Sbjct: 6   NYLNLVTLLLF-FAISDSFGGNLDDFLRCV----SNDDSNPILINTPSSSTYSSVLNFTV 65

Query: 67  RNLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVAN-IPF 126
           RNLRFSGP+ PKP AII PSQ S VQ  VIC K H +HIR+RSGGHDFEGLSYVA+ +PF
Sbjct: 66  RNLRFSGPDTPKPFAIINPSQASHVQGGVICAKNHGVHIRVRSGGHDFEGLSYVAHGVPF 125

Query: 127 LLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSG 186
           +LID+A LN+V+V+ DDNTAWV+SGASMGELYYKLG +TS FGFPGGVQPNVGVGGFLSG
Sbjct: 126 VLIDMAKLNSVSVDTDDNTAWVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFLSG 185

Query: 187 GGYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLK 246
           GGYGLMVRKYGLAVDNVVDAQ+VDANG L+DR  MGEDLFWAIRGGGGGSFGIV+AWKLK
Sbjct: 186 GGYGLMVRKYGLAVDNVVDAQVVDANGRLVDRDSMGEDLFWAIRGGGGGSFGIVVAWKLK 245

Query: 247 LVPVPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT-------------- 306
           LVPVP IVTSFT F+ WDQNST LVH+WQ VAPEIDERLFIG MVT              
Sbjct: 246 LVPVPSIVTSFTAFRIWDQNSTNLVHKWQLVAPEIDERLFIGAMVTAANSGEGGGRIMGV 305

Query: 307 -------GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDRT 366
                  GRA+E+ISIM  +FPE+GL+ EDCVESNWVESMAYPASGFVT KDMNFL+DRT
Sbjct: 306 FLYSLFLGRAEEVISIMENNFPEVGLRMEDCVESNWVESMAYPASGFVTAKDMNFLLDRT 365

Query: 367 PLSNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPS 426
           PL+NGRYKTKSDYATEPIS+TA  GIWERF NAKGI+TVQL+LIPYGGKMN+FSET+TPS
Sbjct: 366 PLTNGRYKTKSDYATEPISQTALHGIWERF-NAKGIQTVQLVLIPYGGKMNKFSETDTPS 425

Query: 427 PHRAGYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNN 486
           PHRA YPIKIAYYVTW++ EGDSRHLEWTRELY+YMTPFVSKSP+AAYVNYRDLDIGRNN
Sbjct: 426 PHRARYPIKIAYYVTWDTLEGDSRHLEWTRELYDYMTPFVSKSPKAAYVNYRDLDIGRNN 485

Query: 487 EDGIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPVA 513
           E+GIPTSYEEA VWGLKYFGDNFERLVEVK KVDP+N+FRHEQSIPPVA
Sbjct: 486 EEGIPTSYEEAGVWGLKYFGDNFERLVEVKTKVDPYNLFRHEQSIPPVA 528

BLAST of Lsi02G014560 vs. ExPASy TrEMBL
Match: A0A6J1HP64 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.3e-211
Identity = 360/538 (66.91%), Postives = 428/538 (79.55%), Query Frame = 0

Query: 7   NYFSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTI 66
           N   F+SLLLLSF  W C  DNL + LHC+S H S + SNSTLI+ PSSSSY +VLNF+I
Sbjct: 27  NIEDFISLLLLSFASWVCGSDNLQDFLHCISLHSSNHHSNSTLIHTPSSSSYPHVLNFSI 86

Query: 67  RNLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPFL 126
           RNLRF+  + PKP+AI+ P QPS+VQ TVICCK H + IR RSGGHDFEG SYV+N+PF+
Sbjct: 87  RNLRFAESHTPKPVAIVTPWQPSEVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNVPFV 146

Query: 127 LIDLANLNAVTVEDDN-TAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGG 186
           LIDL NLN++T++ DN TAW QSGA++GELYYK+G+++ +  FP G   ++G+GGF SGG
Sbjct: 147 LIDLINLNSITIDVDNETAWAQSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSGG 206

Query: 187 GYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKL 246
           G G++VRKYGLAVDNVVDA LVDANG+LLDR  MGEDLFWAIRGGGGGSFGIVLAWKLKL
Sbjct: 207 GLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKL 266

Query: 247 VPVPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT--------------- 306
           V VPP VTSF   KSWD N+ KL+HRWQYVAP++DE LFI   VT               
Sbjct: 267 VRVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPKVDEDLFITAWVTASNSSEGEGGGRVMK 326

Query: 307 --------GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDR 366
                   G+A EL+S+M K+FPEL LKKEDC+E +WVE+MA+ AS +V+ +++  L+DR
Sbjct: 327 ASFFSLFLGKAAELVSLMKKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDR 386

Query: 367 TPLSNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTP 426
           TPL  GRYK KSDYAT+PISETA  GIWERF   + +ETVQLILIP+GGKMNE SET TP
Sbjct: 387 TPLITGRYKMKSDYATKPISETALNGIWERF-KIEELETVQLILIPFGGKMNEISETATP 446

Query: 427 SPHRAGYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRN 486
           +PHRA +PI I YYVTW+ P+ DSRHLEWTREL++YMTPFVS+SPRAAY+NY+DLDIGRN
Sbjct: 447 NPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGRN 506

Query: 487 NEDGIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPVA-RGGIQAK 520
           NEDGIPTSY+EASVWG +YFG NFERLVEVK KVDPFN F+HE+SIPP A  G IQAK
Sbjct: 507 NEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFQHEESIPPAAPMGVIQAK 563

BLAST of Lsi02G014560 vs. ExPASy TrEMBL
Match: A0A6J1HYZ7 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.4e-210
Identity = 360/533 (67.54%), Postives = 426/533 (79.92%), Query Frame = 0

Query: 10  SFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRNL 69
           +F+SLLLLSF  W    DNL++ LHC+S H S   SNSTLI+ PSSSSY YVLNF+IRNL
Sbjct: 25  NFISLLLLSFASWGFGSDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRNL 84

Query: 70  RFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPFLLID 129
           RF+  + PKP+AI+ P Q SQVQ TVICCK H + IR RSGGHDFEG SYV+NIPF+LID
Sbjct: 85  RFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLID 144

Query: 130 LANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGYG 189
           L NLN++T++ DD TAW QSGA++GELYYK+G+++ +  FP G+  ++G+GGF SGGG G
Sbjct: 145 LINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLG 204

Query: 190 LMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVPV 249
           ++VRKYGLAVDNVVDA LVDANG+LLDR  MGEDLFWAIRGGGGGSFGIVLAWKLKLV V
Sbjct: 205 MLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRV 264

Query: 250 PPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT------------------ 309
           PP VTSF   KSWD N+ KL+H WQYVA E+DE LFI   VT                  
Sbjct: 265 PPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFFS 324

Query: 310 ---GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDRTPLSN 369
              G+A EL+S+M K+FPEL LKKEDC+E +WVE+MA+ AS +V+ +++  L+DRT L  
Sbjct: 325 LFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLIT 384

Query: 370 GRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPSPHRA 429
           GRYK KSDYAT+PISETA  GIWERF   + +ETVQLILIP+GGKMNE SET+TP+PHRA
Sbjct: 385 GRYKMKSDYATKPISETALNGIWERF-KIEELETVQLILIPFGGKMNEISETDTPNPHRA 444

Query: 430 GYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNEDGI 489
            +PI I YYVTW+ P+ DSRHLEWTREL++YMTPFVS+SPRAAY+NY+DLDIG+NNEDGI
Sbjct: 445 SHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGI 504

Query: 490 PTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPVA-RGGIQAK 520
           PTSY+EASVWG +YFG NFERLVEVK KVDPFN FRHEQSIPP A  GGIQAK
Sbjct: 505 PTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPAAPMGGIQAK 556

BLAST of Lsi02G014560 vs. ExPASy TrEMBL
Match: A0A6J1EVN7 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111438506 PE=3 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.8e-210
Identity = 360/535 (67.29%), Postives = 425/535 (79.44%), Query Frame = 0

Query: 9   FSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRN 68
           FSF+SL+ +SF  W C  DNL + LHC+S H S ++SNSTLI+ PSSSSY YVLNF+IRN
Sbjct: 15  FSFISLVFVSFASWGCGSDNLQDFLHCISLHSSNHNSNSTLIHTPSSSSYLYVLNFSIRN 74

Query: 69  LRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPFLLI 128
           LRF+  + PKP+AI+ P Q S+VQTTVICCK H + IR RSGG DFEG SYV+N+PF+LI
Sbjct: 75  LRFAESDTPKPVAIVTPWQASEVQTTVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFVLI 134

Query: 129 DLANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGY 188
           DL NLN++T++ +D TAW QSGA++GELYYK+G+++ +  FP G+  N+G+GGF SGGG+
Sbjct: 135 DLTNLNSITIDTEDETAWAQSGATVGELYYKIGEKSRTLAFPAGLVANLGLGGFFSGGGF 194

Query: 189 GLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 248
           G++VRKYGLAVDNVVDAQLVDANG+LLDR  MGEDLFWAIRGGGGGSFGIVL WKLKLV 
Sbjct: 195 GMLVRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKLVR 254

Query: 249 VPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMV------------------ 308
           VPP VTSF   KSWD N+ KL+HRWQYVAPE DE LFI   V                  
Sbjct: 255 VPPTVTSFAVHKSWDLNAAKLIHRWQYVAPEADEDLFITAWVIASNSSDGGGRIMKASFF 314

Query: 309 ---TGRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDR-TPL 368
               G A EL+S+M K+FPEL L KEDC+E +WVESMA+ AS FV+ + +  L+DR T L
Sbjct: 315 SLFLGEAAELVSLMAKAFPELELTKEDCLEMSWVESMAFSASVFVSAESLELLLDRTTQL 374

Query: 369 SNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPSPH 428
           SNGRYK KSDYATEPISETA  GIWERF   + +ET+QL+LIP+GGKMNE SET+TP+PH
Sbjct: 375 SNGRYKIKSDYATEPISETALNGIWERF-KIEELETLQLVLIPFGGKMNEISETDTPNPH 434

Query: 429 RAGYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNED 488
           RA +PI I YY TW+ P+ DSRHLEWTREL++YMTPFVSKSPRAAY+N +DLDIGRNNED
Sbjct: 435 RARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNNED 494

Query: 489 GIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPVAR-GGIQAK 520
           GIPTSYEEAS+WG +YFG NFERLV VK K DPFN FRHEQSIPPVA  GGIQAK
Sbjct: 495 GIPTSYEEASIWGHQYFGINFERLVAVKTKADPFNFFRHEQSIPPVAPIGGIQAK 548

BLAST of Lsi02G014560 vs. ExPASy TrEMBL
Match: A0A6J1I038 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 4.1e-210
Identity = 360/535 (67.29%), Postives = 426/535 (79.63%), Query Frame = 0

Query: 9   FSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRN 68
           F FVSL+ +SF  W C  DNL + LHC+S H S ++SNSTLI+ PSSSSY YVLNF+IRN
Sbjct: 15  FGFVSLVFVSFASWGCGSDNLQDFLHCISLHSSNHNSNSTLIHTPSSSSYLYVLNFSIRN 74

Query: 69  LRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPFLLI 128
           LRF+  + PKP+AI+ P Q SQVQ TVICCK H + IR RSGG DFEG SYV+N+PF+LI
Sbjct: 75  LRFAESDTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFVLI 134

Query: 129 DLANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGY 188
           DL NLN++T++ +D TAW QSGA++GELYYK+GQ++ +  FP G+  N+G+GGF SGGG+
Sbjct: 135 DLINLNSITIDTEDETAWAQSGATVGELYYKIGQKSRTLAFPAGLVANLGLGGFFSGGGF 194

Query: 189 GLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 248
           G+++RKYGLAVDNVVDAQLVDANG+LLDR  MGEDLFWAIRGGGGGSFGIVL WKLKLV 
Sbjct: 195 GMLLRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKLVR 254

Query: 249 VPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT----------------- 308
           VPP VTSF   KSWD N+ KL+H+WQYVAPE+DE LFI   VT                 
Sbjct: 255 VPPTVTSFAVHKSWDLNAAKLIHQWQYVAPEVDEDLFITAWVTASNSSDGGGRIMKASFF 314

Query: 309 ----GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDR-TPL 368
               G A EL+S+M K+FPEL L +EDC+E +WVESMA+ AS FV+ +++  L+DR T L
Sbjct: 315 ALFLGEAAELVSLMAKAFPELELTREDCLEMSWVESMAFSASVFVSAENLELLLDRTTQL 374

Query: 369 SNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPSPH 428
           SNGRYK KSDYATEPISETA  GIWERF   + +ET+QLILIP+GGKMNE SET+TP+PH
Sbjct: 375 SNGRYKIKSDYATEPISETALNGIWERF-KIEELETLQLILIPFGGKMNEISETDTPNPH 434

Query: 429 RAGYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNED 488
           RA +PI I YY TW+ P+ DSRHLEWTREL++YMTPFVSKSPRAAY+N +DLDIGRNNED
Sbjct: 435 RARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNNED 494

Query: 489 GIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPVAR-GGIQAK 520
           GIPTSYEEAS+WG +YFG NFERLVEVK K DPFN FRHEQSIPPVA   GIQAK
Sbjct: 495 GIPTSYEEASIWGHQYFGINFERLVEVKTKADPFNFFRHEQSIPPVAPIRGIQAK 548

BLAST of Lsi02G014560 vs. NCBI nr
Match: XP_022964765.1 (berberine bridge enzyme-like 26 [Cucurbita moschata])

HSP 1 Score: 828.6 bits (2139), Expect = 3.1e-236
Identity = 410/529 (77.50%), Postives = 451/529 (85.26%), Query Frame = 0

Query: 7   NYFSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTI 66
           NY + V+LLL  F I D  G NLD+ L CV    S  DSN  LI  PSSS+YS VLNFT+
Sbjct: 6   NYLNLVTLLLF-FAISDSFGGNLDDFLRCV----SNDDSNPILINTPSSSTYSSVLNFTV 65

Query: 67  RNLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVAN-IPF 126
           RNLRFSGP+ PKP AII PSQ S VQ  VIC K H +HIR+RSGGHDFEGLSYVA+ +PF
Sbjct: 66  RNLRFSGPDTPKPFAIINPSQASHVQGGVICAKNHGVHIRVRSGGHDFEGLSYVAHGVPF 125

Query: 127 LLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSG 186
           +LID+A LN+V+V+ DDNTAWV+SGASMGELYYKLG +TS FGFPGGVQPNVGVGGFLSG
Sbjct: 126 VLIDMAKLNSVSVDTDDNTAWVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFLSG 185

Query: 187 GGYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLK 246
           GGYGLMVRKYGLAVDNVVDAQ+VDANG L+DR  MGEDLFWAIRGGGGGSFGIV+AWKLK
Sbjct: 186 GGYGLMVRKYGLAVDNVVDAQVVDANGRLVDRDSMGEDLFWAIRGGGGGSFGIVVAWKLK 245

Query: 247 LVPVPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT-------------- 306
           LVPVP IVTSFT F+ WDQNST LVH+WQ VAPEIDERLFIG MVT              
Sbjct: 246 LVPVPSIVTSFTAFRIWDQNSTNLVHKWQLVAPEIDERLFIGAMVTAANSGEGGGRIMGV 305

Query: 307 -------GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDRT 366
                  GRA+E+ISIM  +FPE+GL+ EDCVESNWVESMAYPASGFVT KDMNFL+DRT
Sbjct: 306 FLYSLFLGRAEEVISIMENNFPEVGLRMEDCVESNWVESMAYPASGFVTAKDMNFLLDRT 365

Query: 367 PLSNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPS 426
           PL+NGRYKTKSDYATEPIS+TA  GIWERF NAKGI+TVQL+LIPYGGKMN+FSET+TPS
Sbjct: 366 PLTNGRYKTKSDYATEPISQTALHGIWERF-NAKGIQTVQLVLIPYGGKMNKFSETDTPS 425

Query: 427 PHRAGYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNN 486
           PHRA YPIKIAYYVTW++ EGDSRHLEWTRELY+YMTPFVSKSP+AAYVNYRDLDIGRNN
Sbjct: 426 PHRARYPIKIAYYVTWDTLEGDSRHLEWTRELYDYMTPFVSKSPKAAYVNYRDLDIGRNN 485

Query: 487 EDGIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPVA 513
           E+GIPTSYEEA VWGLKYFGDNFERLVEVK KVDP+N+FRHEQSIPPVA
Sbjct: 486 EEGIPTSYEEAGVWGLKYFGDNFERLVEVKTKVDPYNLFRHEQSIPPVA 528

BLAST of Lsi02G014560 vs. NCBI nr
Match: XP_023521064.1 (berberine bridge enzyme-like 26 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 820.8 bits (2119), Expect = 6.4e-234
Identity = 402/509 (78.98%), Postives = 440/509 (86.44%), Query Frame = 0

Query: 28  NLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRNLRFSGPNIPKPIAIIKPSQ 87
           NLD+ L CV    S  DSN  LI  PSSS+YS VLNFT+RNLRFSGPN PKP AII PSQ
Sbjct: 3   NLDDFLRCV----SNDDSNPILINTPSSSTYSSVLNFTVRNLRFSGPNTPKPCAIINPSQ 62

Query: 88  PSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVAN-IPFLLIDLANLNAVTVE-DDNTAW 147
            S VQ  VIC K H +HIR+RSGGHDFEGLSYVA+ + F+LID+A LN+V+V+ DDNTAW
Sbjct: 63  ASHVQAGVICAKNHGVHIRVRSGGHDFEGLSYVAHGVAFVLIDMAKLNSVSVDTDDNTAW 122

Query: 148 VQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNVVDAQ 207
           V+SGASMGELYYKLG +TS FGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNVVDAQ
Sbjct: 123 VESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNVVDAQ 182

Query: 208 LVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFTTFKSWDQNS 267
           +VDANG ++DR  MGEDLFWAIRGGGGGSFGIV+AWKLKLVPVP IVTSFT F+ WDQNS
Sbjct: 183 VVDANGRVVDRDSMGEDLFWAIRGGGGGSFGIVVAWKLKLVPVPSIVTSFTAFRIWDQNS 242

Query: 268 TKLVHRWQYVAPEIDERLFIGIMVT----------------------GRADELISIMNKS 327
           T LVH+WQ VAPEIDERLFIG MVT                      GRA+E+ISIM  +
Sbjct: 243 TNLVHKWQLVAPEIDERLFIGAMVTAANSGEGGGGRIMGVFLYSLFLGRAEEVISIMENN 302

Query: 328 FPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDRTPLSNGRYKTKSDYATEPISE 387
           FPE+GL+ EDCVESNWVESMAYPASGFVT KDMNFL+DRTPL+NGRYKTKSDYATEPIS+
Sbjct: 303 FPEVGLRMEDCVESNWVESMAYPASGFVTAKDMNFLLDRTPLTNGRYKTKSDYATEPISQ 362

Query: 388 TAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPSPHRAGYPIKIAYYVTWNSPE 447
           TA  GIWERF NAKGI+TVQL+LIPYGGKMN+FSET+TPSPHRA YPIKIAYYVTW++PE
Sbjct: 363 TALHGIWERF-NAKGIQTVQLVLIPYGGKMNKFSETDTPSPHRARYPIKIAYYVTWDTPE 422

Query: 448 GDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKYFG 507
           GDSRHLEWTRELY+YMTPFVSKSPRAAYVNYRDLDIGRNNE+GIPTSYEEASVWGLKYFG
Sbjct: 423 GDSRHLEWTRELYDYMTPFVSKSPRAAYVNYRDLDIGRNNEEGIPTSYEEASVWGLKYFG 482

Query: 508 DNFERLVEVKRKVDPFNVFRHEQSIPPVA 513
           DNFERLVEVK KVDP+N+FRHEQSIPPVA
Sbjct: 483 DNFERLVEVKTKVDPYNLFRHEQSIPPVA 506

BLAST of Lsi02G014560 vs. NCBI nr
Match: XP_023525672.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 798.5 bits (2061), Expect = 3.4e-227
Identity = 387/535 (72.34%), Postives = 439/535 (82.06%), Query Frame = 0

Query: 6   RNYFSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFT 65
           RNY +F+S +L+S L+W C G NL++ LHC+S +     SNSTL+Y  SSSSYS VLNFT
Sbjct: 5   RNYVNFIS-VLVSLLLWGCWGSNLEDFLHCISSN----SSNSTLLYTQSSSSYSSVLNFT 64

Query: 66  IRNLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPF 125
           IRNLRFSGPN+PKPIAIIKPSQPSQVQ  VICC  HD+ IR+RSGGHDFEGLSYVAN+ F
Sbjct: 65  IRNLRFSGPNVPKPIAIIKPSQPSQVQAAVICCNNHDLRIRVRSGGHDFEGLSYVANVTF 124

Query: 126 LLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSG 185
           ++IDL NLN+VTV+ ++NTAWVQSGA+ GE+YYKLG ++S+FGFPGGVQP VG+GGFLSG
Sbjct: 125 VVIDLVNLNSVTVDIENNTAWVQSGATQGEVYYKLGLKSSAFGFPGGVQPTVGIGGFLSG 184

Query: 186 GGYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLK 245
           GGYG+MVRKYGL  DNVVDA LVDANG LLDR  MGEDLFWAIRGGGGGSFGIVLAWKLK
Sbjct: 185 GGYGMMVRKYGLGADNVVDALLVDANGRLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLK 244

Query: 246 LVPVPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT-------------- 305
           LVPVPP VTSF  ++S DQN+T LVHRWQ+VAP IDERLFIG++V+              
Sbjct: 245 LVPVPPTVTSFAAYRSLDQNATDLVHRWQFVAPVIDERLFIGVIVSGLILILFFFRGGGR 304

Query: 306 ------------GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNF 365
                       G+A+ELI IM KSFPELGLKK DC+E+NWVES AYPASGFVT  D+N 
Sbjct: 305 RTKGAFFYSLFLGKAEELIPIMEKSFPELGLKKADCLETNWVESTAYPASGFVTANDLNV 364

Query: 366 LIDRTPLSNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSE 425
           L+DR PLS+GR+KTKSDY TEPISE A  GIWER  +A GIETVQL+LIPYGGKMNE +E
Sbjct: 365 LLDRDPLSSGRFKTKSDYITEPISEAALDGIWERL-DADGIETVQLVLIPYGGKMNEIAE 424

Query: 426 TNTPSPHRAGYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLD 485
           T  PSPHR GYPIKI YYVTW SPE D RHLEW+RE+YEYM PFVSKSPRAAYVNYRDLD
Sbjct: 425 TELPSPHRDGYPIKIGYYVTWESPEADMRHLEWSREVYEYMAPFVSKSPRAAYVNYRDLD 484

Query: 486 IGRNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPVAR 514
           IG NNEDG PTS+EEASVWGLKYFG NFERLV VK  VDP N+FRH+QSIPP+A+
Sbjct: 485 IGTNNEDGTPTSFEEASVWGLKYFGVNFERLVRVKTMVDPCNLFRHQQSIPPMAQ 533

BLAST of Lsi02G014560 vs. NCBI nr
Match: KAG6583521.1 (Berberine bridge enzyme-like 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 798.5 bits (2061), Expect = 3.4e-227
Identity = 380/451 (84.26%), Postives = 414/451 (91.80%), Query Frame = 0

Query: 64  FTIRNLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVAN- 123
           F I   RFSGPN PKP AII PSQ S VQ  VIC K H +HIR+RSGGHDFEGLSYVA+ 
Sbjct: 504 FGINFQRFSGPNTPKPFAIINPSQASHVQGGVICAKNHGVHIRVRSGGHDFEGLSYVAHG 563

Query: 124 IPFLLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGF 183
           +PF+LID+A LN+V+V+ DDNTAWV+SGASMGELYYKLG +TS FGFPGGVQPNVGVGGF
Sbjct: 564 VPFVLIDMAKLNSVSVDTDDNTAWVESGASMGELYYKLGLETSGFGFPGGVQPNVGVGGF 623

Query: 184 LSGGGYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAW 243
           LSGGGYGLMVRKYGLAVDNVVDAQ+VDANG L+DR  MGEDLFWAIRGGGGGSFGIV+AW
Sbjct: 624 LSGGGYGLMVRKYGLAVDNVVDAQVVDANGRLVDRDSMGEDLFWAIRGGGGGSFGIVVAW 683

Query: 244 KLKLVPVPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMVTGRADELISIMN 303
           KLKLVPVP IVTSFT F+ WDQNST LVH+WQ VAPEIDERLFIG MVTGRA+E+ISIM 
Sbjct: 684 KLKLVPVPSIVTSFTAFRIWDQNSTNLVHKWQLVAPEIDERLFIGAMVTGRAEEVISIME 743

Query: 304 KSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDRTPLSNGRYKTKSDYATEPI 363
            +FPE+GL+ EDCVESNWVESMAYPASGFVT KDMNFL+DRTPL+NGRYKTKSDYATEPI
Sbjct: 744 NNFPEVGLRMEDCVESNWVESMAYPASGFVTAKDMNFLLDRTPLTNGRYKTKSDYATEPI 803

Query: 364 SETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPSPHRAGYPIKIAYYVTWNS 423
           S+TA  GIWERF NAKGI+TVQL+LIPYGGKMN+FSET+TPSPHRA YPIKIAYYVTW++
Sbjct: 804 SQTALHGIWERF-NAKGIQTVQLVLIPYGGKMNKFSETDTPSPHRARYPIKIAYYVTWDT 863

Query: 424 PEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVWGLKY 483
           PEGDSRHLEWTRELY+YMTPFVSKSPRAAYVNYRDLDIGRNNE+GIPTSYEEASVWGLKY
Sbjct: 864 PEGDSRHLEWTRELYDYMTPFVSKSPRAAYVNYRDLDIGRNNEEGIPTSYEEASVWGLKY 923

Query: 484 FGDNFERLVEVKRKVDPFNVFRHEQSIPPVA 513
           FGDNFERLVEVK KVDP+N+FRHEQSIPPVA
Sbjct: 924 FGDNFERLVEVKTKVDPYNLFRHEQSIPPVA 953

BLAST of Lsi02G014560 vs. NCBI nr
Match: KAG6583521.1 (Berberine bridge enzyme-like 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 402.5 bits (1033), Expect = 5.4e-108
Identity = 192/283 (67.84%), Postives = 229/283 (80.92%), Query Frame = 0

Query: 9   FSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRN 68
           FSF+SLLLLSF  W C  DNL + LHC+S H S + SNSTLI+ PSSSSY +VLNF+IRN
Sbjct: 8   FSFISLLLLSFASWGCGSDNLQDFLHCISLHSSNHHSNSTLIHTPSSSSYPHVLNFSIRN 67

Query: 69  LRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPFLLI 128
           LRF+  + PKP+AI+ P QPS+VQ  VICCK H + IR RSGGHDFEG SYV+N+PF+LI
Sbjct: 68  LRFAESHTPKPVAIVTPWQPSEVQAIVICCKTHGLQIRTRSGGHDFEGRSYVSNVPFVLI 127

Query: 129 DLANLNAVTVEDDN-TAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGY 188
           DL NLN++ ++ DN TAW QSGA++GELYYK+G+++ +  FP G   ++G+GGF SGGG 
Sbjct: 128 DLINLNSIIIDVDNETAWAQSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSGGGL 187

Query: 189 GLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 248
           G++VRKYGLAVDNVVDA LVDANG+LLDR  MGEDLFWAIRGGGGGSFGIVLAWKLKLV 
Sbjct: 188 GMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVR 247

Query: 249 VPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT 291
           VPP VTSF   KSWD  + KL+HRWQYVAP++DE LFI   VT
Sbjct: 248 VPPTVTSFAVHKSWDLKAAKLIHRWQYVAPKVDEDLFITAWVT 290

HSP 2 Score: 768.8 bits (1984), Expect = 2.9e-218
Identity = 372/506 (73.52%), Postives = 419/506 (82.81%), Query Frame = 0

Query: 6   RNYFSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFT 65
           RNY +F+S +L+S L+W C G NL++ LHC+S +     SNSTL+Y  SSSSYS VLNFT
Sbjct: 5   RNYVNFIS-VLVSLLLWGCWGSNLEDFLHCISSN----SSNSTLLYTQSSSSYSSVLNFT 64

Query: 66  IRNLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPF 125
           IRNLRFSGPN+PKPIAIIKPSQPSQVQ  VICC  HD+ IR+RSGGHDFEGLSYVAN+ F
Sbjct: 65  IRNLRFSGPNVPKPIAIIKPSQPSQVQAAVICCNNHDLRIRVRSGGHDFEGLSYVANVTF 124

Query: 126 LLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSG 185
           ++IDL NLN+VTV+ ++NTAWVQSGA+ GE+YYKLGQ+TS+FGFPGGVQP VG+GGFLSG
Sbjct: 125 VVIDLVNLNSVTVDTENNTAWVQSGATQGEVYYKLGQKTSAFGFPGGVQPTVGIGGFLSG 184

Query: 186 GGYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLK 245
           GGYG+MVR+YGL  DNVVDA LVDANG LLDR  MGEDLFWAIRGGGGGSFGIVLAWKLK
Sbjct: 185 GGYGMMVRRYGLGADNVVDALLVDANGRLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLK 244

Query: 246 LVPVPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMVT-------------- 305
           LVPVPP VTSF  ++S DQN+T LVHRWQ+VAP IDERLFIG++V+              
Sbjct: 245 LVPVPPTVTSFAAYRSLDQNATDLVHRWQFVAPVIDERLFIGVIVSAANSMEGGGGRTKG 304

Query: 306 --------GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDR 365
                   G+A+ELI IM KSFPELGLKKEDC+E+NWVES AYPASGFVT  D+N L+DR
Sbjct: 305 AFFYSLFLGKAEELIPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDR 364

Query: 366 TPLSNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTP 425
            PLS+GRYKTKSDY TEPISE A  GIWER  +  GIETVQL+LIPYGGKMNE +ET  P
Sbjct: 365 APLSSGRYKTKSDYITEPISEAALDGIWERL-DGDGIETVQLVLIPYGGKMNEIAETELP 424

Query: 426 SPHRAGYPIKIAYYVTWNSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRN 485
           SPHRAGYPIKI YYVTW S E D RHLEW+RE+YEYM PFVSKSPRAAYVNYRDLDIG N
Sbjct: 425 SPHRAGYPIKIGYYVTWESGEADMRHLEWSREVYEYMAPFVSKSPRAAYVNYRDLDIGTN 484

Query: 486 NEDGIPTSYEEASVWGLKYFGDNFER 489
           NEDG PTS+EEASVWGLKYFG NFER
Sbjct: 485 NEDGTPTSFEEASVWGLKYFGVNFER 504

BLAST of Lsi02G014560 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 471.1 bits (1211), Expect = 1.2e-132
Identity = 245/531 (46.14%), Postives = 338/531 (63.65%), Query Frame = 0

Query: 13  SLLLLSFLIW-----DCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSS--SSYSYVLNFT 72
           S+L+L F ++       L    D  ++CV  +   Y       + P+   S +S VL  T
Sbjct: 11  SILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLEST 70

Query: 73  IRNLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANI-- 132
            +NLRF   ++PKP  I  P   S VQ ++IC KK  +H+R+RSGGHD+EGLSYV+ I  
Sbjct: 71  AQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDK 130

Query: 133 PFLLIDLANLNAVTVE-DDNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFL 192
           PF+L+DL+ +  V +   DN+AWVQSGA++GELYY++ +++   GFP G+  ++G+GG +
Sbjct: 131 PFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHI 190

Query: 193 SGGGYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWK 252
           +GG YG M+RKYGL  DNV+DA++VDANG+LLDR  MGED FWAIRGG GGSFGI+LAWK
Sbjct: 191 TGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWK 250

Query: 253 LKLVPVPPIVTSFTTFKSWDQN-STKLVHRWQYVAPEIDERLFIGIMVT----------- 312
           +KLVPVP  VT FT  K+  Q+   K++ +WQ VA ++ E LFI ++             
Sbjct: 251 IKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVT 310

Query: 313 --------GRADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLID- 372
                   G    L+++M KSFPELGL  +DC+E +W+ES+AY  SGF T    N L+  
Sbjct: 311 TSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAY-ISGFPTHTPTNVLLQG 370

Query: 373 RTPLSNGRYKTKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNT 432
           ++P     +K KSD+   PI E+  QGI+++       +   +I  PYGG M +  E+  
Sbjct: 371 KSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKE---DIPLMIWNPYGGMMAKIPESQI 430

Query: 433 PSPHRAGYPIKIAYYVTW-NSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIG 492
           P PHR G   K+ Y  +W +S +  SRH+ W R+LY YMTP+VS +PR AYVNYRDLD+G
Sbjct: 431 PFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLG 490

Query: 493 RNNEDGIPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIPPV 512
           RN +D + T  ++A VWG  YF +NF RL+ +K KVDP N FRHEQSIPP+
Sbjct: 491 RNTKD-VKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Lsi02G014560 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 463.8 bits (1192), Expect = 1.9e-130
Identity = 231/500 (46.20%), Postives = 329/500 (65.80%), Query Frame = 0

Query: 28  NLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRNLRFSGPNIPKPIAIIKPSQ 87
           N  + L C+S   +  +  S +I+ P+ +S+S VL  +I+N RFS P++PKP+ I+ P Q
Sbjct: 33  NQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQ 92

Query: 88  PSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANIPFLLIDLANLNAVTVEDDN-TAWV 147
           PS VQ+ V C ++  IHIR RSGGHD+EGLSYV + PF+++DL NL ++TV+ DN + WV
Sbjct: 93  PSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWV 152

Query: 148 QSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDNVVDAQL 207
           Q+GA++GELYY++G++  +  FP GV P VGVGG  SGGGYG ++RK+GLA D+V+DA++
Sbjct: 153 QTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARV 212

Query: 208 VDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFTTFKSWDQNST 267
           VDA G +L+R+ MGED FWAIRGGGG SF +VL+WK+ L+ VP  VT F   K  +Q++ 
Sbjct: 213 VDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSAL 272

Query: 268 KLVHRWQYVAPEIDERLFIGIMV---------------TGRADELISIMNKSFPELGLKK 327
           K++HRWQ+VA ++ + LFI +M+                G    L+ ++NK FPELGL++
Sbjct: 273 KIIHRWQFVADKVSDDLFIRVMLQRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEE 332

Query: 328 EDCVESNWVESMAYPASGFVTTKDMNFLIDRTPLSNGRYKTKSDYATEPISETAFQGIWE 387
           +DC E +W+ES+ + A   +  + +N L  RT  S   +K KSD+  EP+ +TA   +W 
Sbjct: 333 DDCTEMSWIESVIWFAE--LGEEPINVLTKRTRASLA-FKAKSDFVQEPMPKTAISKLWR 392

Query: 388 RFNNAKGIETVQLILIPYGGKMNEFSETNTPSPHRAGYPIKIAYYVTWNSPEGDSRHLEW 447
           R    +  E  QLI  P+GGKM+E ++  TP PHR G   +I Y   W   +   +++ W
Sbjct: 393 RLQEPEA-EHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG-DVKEKYMRW 452

Query: 448 TRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNEDGIPTS-YEEASVWGLKYFGDNFERLV 507
              +Y+ M+ FV+KSPR AY+N RDLD+G     G+  S YEE   WG+KYF +NFERLV
Sbjct: 453 VERVYDDMSEFVAKSPRGAYINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKNNFERLV 512

Query: 508 EVKRKVDPFNVFRHEQSIPP 511
            VK  VDP + F  EQSIPP
Sbjct: 513 RVKTSVDPSDFFCDEQSIPP 525

BLAST of Lsi02G014560 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 463.0 bits (1190), Expect = 3.2e-130
Identity = 235/523 (44.93%), Postives = 330/523 (63.10%), Query Frame = 0

Query: 8   YFSFVSLLLLSFLIWDCLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIR 67
           YF  ++L L  F        N +N L C+S   ++ DS   +I+     SY  +LN +I+
Sbjct: 13  YFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDSR--IIHTSKDPSYFSILNSSIQ 72

Query: 68  NLRFSGPNIPKPIAIIKPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVA-NIPFL 127
           N RF     PKP++II P Q + VQ+T+ C + H IHIR RSGGHD+EGLSY+A + PF+
Sbjct: 73  NPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFV 132

Query: 128 LIDLANLNAVTVEDDN-TAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGG 187
           +IDL NL ++T++ DN T WVQSGA++GELYY++G+ + S  FP G+ P VG+GG   GG
Sbjct: 133 VIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGG 192

Query: 188 GYGLMVRKYGLAVDNVVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKL 247
           GYG ++RKYGL+ DNV+DA +VDANG  LDR+ MGED FWAIRGGGG SF +VL+WK++L
Sbjct: 193 GYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRL 252

Query: 248 VPVPPIVTSFTTFKSWDQNSTKLVHRWQYVAPEIDERLFIGIMV--------------TG 307
           + VP +VT F   K+ ++ +  ++++WQY+A ++   LFI  M+               G
Sbjct: 253 LDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETEVYASFPGLYLG 312

Query: 308 RADELISIMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDRTPLSNGRYK 367
              +L+++M   FPELGL+  +C E +W+ES+ +    F+  + M  L  R   S   +K
Sbjct: 313 PVSDLLALMKDKFPELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKRTSRS-FK 372

Query: 368 TKSDYATEPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPSPHRAGYPI 427
            K D+  EPI +TA Q +W RF  A      ++IL P+GGKM+E ++   P PHR G   
Sbjct: 373 GKDDFIEEPIPKTAIQYLWRRF-EAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLY 432

Query: 428 KIAYYVTWNSPE-----GDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNEDG 487
           +I Y   W+  E        ++L W   +YE+MTP+VSKSPR AYVN+RD+D+G      
Sbjct: 433 EIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLN 492

Query: 488 IPTSYEEASVWGLKYFGDNFERLVEVKRKVDPFNVFRHEQSIP 510
           + T YEEA VWG+KYF +NF+RLV VK  VDP + F  EQSIP
Sbjct: 493 MKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of Lsi02G014560 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 456.1 bits (1172), Expect = 3.9e-128
Identity = 227/512 (44.34%), Postives = 326/512 (63.67%), Query Frame = 0

Query: 24  CLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRNLRFSGPNIPKPIAII 83
           CL DN D V   ++  +   D N+TL        +   L  T +NLR+  P+ PKP+ I 
Sbjct: 36  CLVDNSD-VSFPITASFFSPDQNATL--------FKEELESTAQNLRYLTPSNPKPVFIF 95

Query: 84  KPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVA--NIPFLLIDLANLNAVTVE-D 143
           +P   + VQ  V+C KK  +H+R+RSGGHD+EGLS+VA    PF+++DL+ L  V V+ D
Sbjct: 96  EPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVDLD 155

Query: 144 DNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDN 203
            N+AW  +GA++GE+YY++ +++ + GFP G+  ++G+GG L GG YG M+RK+GL  DN
Sbjct: 156 SNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGADN 215

Query: 204 VVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFTTFKS 263
           V+DA++VDANG++LDR  MGED+FWAIRGGGGGSFG++LAWK+KLVPVP  VT FT  K+
Sbjct: 216 VLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVTKT 275

Query: 264 WDQNSTKLVHRWQYVAPEIDERLFIGIMVT----------------------GRADELIS 323
            +Q+ TK++++W+ +A ++D+ LFI ++++                      G ++ L+ 
Sbjct: 276 LEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMSYQAQFLGDSNRLLQ 335

Query: 324 IMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDRTPLSNGRYKTKSDYAT 383
           +M KSFPELGL K+DC E +W++S+ Y A GF  +     L+    L    +K KSD+  
Sbjct: 336 VMQKSFPELGLTKKDCTEMSWIKSVMYIA-GFPNSAAPEALLAGKSLFKNHFKAKSDFVK 395

Query: 384 EPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPSPHRAGYPIKIAYYVT 443
           EPI     +G+WERF      ++   I  PYGG M+  SE+  P PHR G   KI +  T
Sbjct: 396 EPIPVEGLEGLWERFLEE---DSPLTIWNPYGGMMSRISESEIPFPHRNGTLFKIQWLST 455

Query: 444 WNSPE-GDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVW 503
           W   +  + RH++W RE+Y YM  +VSK+PR AYVNYRDLD+G N  +       +A  W
Sbjct: 456 WQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGE------TDAREW 515

Query: 504 GLKYFGDNFERLVEVKRKVDPFNVFRHEQSIP 510
           G KY+  NFERLV++K + DP N FRHEQS+P
Sbjct: 516 GAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Lsi02G014560 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 455.3 bits (1170), Expect = 6.6e-128
Identity = 229/512 (44.73%), Postives = 330/512 (64.45%), Query Frame = 0

Query: 24  CLGDNLDNVLHCVSPHYSKYDSNSTLIYIPSSSSYSYVLNFTIRNLRFSGPNIPKPIAII 83
           CL DN D      S  ++ +D N        +SS+   L  + +NLR+  P+ PKP  I 
Sbjct: 38  CLVDNSDASFPMDSSFFT-HDLN--------ASSFKLALETSAQNLRYLMPSNPKPEFIF 97

Query: 84  KPSQPSQVQTTVICCKKHDIHIRIRSGGHDFEGLSYVANI--PFLLIDLANLNAVTVE-D 143
           +P   + VQ  V+C KK  +H+R+RSGGHD+EGLSYV+ +   F+++DL+ L  ++V+ +
Sbjct: 98  EPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLRQISVDIE 157

Query: 144 DNTAWVQSGASMGELYYKLGQQTSSFGFPGGVQPNVGVGGFLSGGGYGLMVRKYGLAVDN 203
            N+AWV +GAS+GE+YY++ +++   GFP G+  ++G+GG + GG YG M+RK+GL  DN
Sbjct: 158 SNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRKFGLGADN 217

Query: 204 VVDAQLVDANGELLDRKLMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFTTFKS 263
           V+DA++VDA+G++L+R  MGED+FWAIRGGGGGSFG++LAWK+KLVPVP IVT FT  ++
Sbjct: 218 VLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVTVFTVTRT 277

Query: 264 WDQNSTKLVHRWQYVAPEIDERLFIGIMV----------------------TGRADELIS 323
            +Q+ TKL+++WQ VA ++DE LFI +++                       G A+ L+ 
Sbjct: 278 LEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSYQGQFLGDANRLLQ 337

Query: 324 IMNKSFPELGLKKEDCVESNWVESMAYPASGFVTTKDMNFLIDRTPLSNGRYKTKSDYAT 383
           +M +SFP+LGL K+DC+E++W++S+ Y A GF +T     L+D   L    +K KSDY  
Sbjct: 338 VMQRSFPQLGLTKKDCLETSWIKSVMYIA-GFPSTAPSEALLDGKSLFKNYFKAKSDYVE 397

Query: 384 EPISETAFQGIWERFNNAKGIETVQLILIPYGGKMNEFSETNTPSPHRAGYPIKIAYYVT 443
           EPI     +G+WE+       ++   I  PYGG M +  ET TP PHR+G   KI +   
Sbjct: 398 EPIPVEGLEGLWEKLLEE---DSPLTIWNPYGGMMAKIPETETPFPHRSGTLFKIQWLTL 457

Query: 444 W-NSPEGDSRHLEWTRELYEYMTPFVSKSPRAAYVNYRDLDIGRNNEDGIPTSYEEASVW 503
           W +    +++H+ W RE+Y YM  +VSKSPR+AYVNYRDLD+G N +        +A  W
Sbjct: 458 WQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGKG------SDAREW 517

Query: 504 GLKYFGDNFERLVEVKRKVDPFNVFRHEQSIP 510
           G +YF  NFERLVE+K K DP N FRHEQSIP
Sbjct: 518 GNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530