Lsi02G014060 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G014060
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMitogen-activated protein kinase
Locationchr02: 19325394 .. 19329203 (-)
RNA-Seq ExpressionLsi02G014060
SyntenyLsi02G014060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGATGACGAAGGAAGAAGCCGTTGAAAAAACGAAAAACGAACACGAACCGTCGATCAAAGGCGGCGGAAATCTGAAACGTCCGATCTTCCGTCAGAAGCGCGTGAGTCATTTTTCTCCCGCGCACGAGCCTTCGTTTCCCTTTCCTCGCATTTACTCTTCACACTCTCTCCTTTTTCCCTTTGACAAATTCTTCTCTGCATAAATATTCATCCAAATTCTTCACTTCTTCTTCAAACTCTTGGTCTTGGTCTTCCTTCTTCATCTGGGGTTTCAATTTCTTGACTGAGAATCGCAGATTTTGATACAGAAGGTGTTTCAGGCAAAGGGTTTCGAAGATTATGGCTGATGTTGGTCAGAACAACCCCGCTGATTTTCCTGCTCTTTCAAGCCATGGTGGTCAATACGTTCAGTACAATATCTTTGGGAATCCTTTCGAAATCACCTCCAAATATCGTCCCCCTATTATGCCTATAGGTCGCGGCGCATACGGAATCGTTTGGTATGTTCATTTCTCTGTTCTGTTTATTTATTTATTTTTTAATTTGTTGCTGTTTTTGTATGAAGCCCCCTTTTGGATTGAGTTTCTTTTCCCTTTTTTTTGGATTTTGTTTGTTTGTAGTTCTGTTTTGAATTCGGAGACCAATGAGATGGTTGCGGTTAAGAAGATTGCTAACGCGTTTGATAACCATATGGATGCGAAGAGGACGCTACGTGAGATTAAGCTTCTTCGCCATTTGGATCATGAAAATGTGAGAGACTTCATCCTTGATGATTTACTAAAACTGTTGTTTCTGTTAATCATTGCGAATTTTGGTTTCTTCCGTTTGTGTTTGTGATTTCTATTTTGATGTGTAAATTGGTGGTCTGGGCTAATGATGATAGAATCCTATGTTATGAAATCAACAAATAAAGAAAGCGAGAAAACGGCGACAAAAAGATTCCCGTGGTTCACTTTTTGTAACACAGATTGTAAGGGAGATAGAGAGTTTATATATAGCACACCCCTCTACACTAAGGCCAAAATCGAAAATAACATAAATACGAGTATAAATTAGTTTTGTTCATTGGTCATTTAGAGATTCAAGGCATTCCAACTTCTTAAAAGGTTGATTTCGATTGAATTCAATTTTGATGTCTACTTATCATTTGGAATACATTGTTTTCGGAGATTTGGTTCATTAATAAGTGGGTTGGACTTTCTCTTTAACTTATAAATGATGACAGTACTTAGCCTCAGCAATGGTTGGGTGATTGGCCATATAATAGTTGAATTACAGCTGTAGTAGCATGTGGTTCTTCATTATTTTGTTGCTGATGGAAAGTTTCTGGGATTTTGACTTTGTTGTTACATTATAATAACTGAATGATGAATTGGAATTGCTTCGTACAACGGTTATCTTGCTTTCTCTGTACTTTCTGATTGTATTTTTATAATATTGGAGGAAGTTTTTTTTAATAGTGTTGTTCTTTAGGGTGACAAGAACAATCTTATATAGATTGTGATTCATTGGATGTAGGTAATAGGCATAAGAGATGTGATTCCTCCACCCTTACGGAGAGAATTCAATGATGTTTACATTTCAACTGAACTAATGGATACCGATCTTCACCAAATAATCCGTTCCAACCAAGGTTTATCGGAAGAGCATTGTCAGGTACGAGGGGGTCTTCACTTTGCCATCTGAACTTGTTCATCATTCATTATGTCAATTGCATCTTTGGTTATTACTTCAATTGTTCCACTTGTAATATGTGGGTTTGGCATGCTTTTTGCAGTATTTCCTTTATCAGATTCTCCGAGGACTGAAATACATACATTCTGCCAATGTCATTCATCGAGACTTGAAACCGAGCAATCTATTGCTTAATGCAAACTGTGATCTTAAAATATGTGATTTTGGTCTTGCTCGACCAACTTCGGAAAATGAAAGTATGACAGAATATGTTGTGACAAGATGGTATAGAGCACCTGAGCTTCTATTGAACTCTGATTATACAGCTGCTATAGATATATGGTCTGTTGGTTGTATCTTTCTGGAGCTTATGAATAGAAGGCCTTTATTTCCAGGCAGGGATCATGTGCATCAGATGCGATTATTGACTGAGGTAAAACAATCTACAGAGAGCTTACGCAGTTTATCAAATTTAGATTATCTAATTTCGACTATTAAAGATCTAACCGATGTGAAAGTATACTCGATGATTTATTGATTCCTGGATAATTTCTACTATCAGTTTTGTTGAGGATGCATCAGTTCAGTACATGGAATGTCTGATGAAAAACCATACGAAGTATATAATCTTAATTTTGTTATGTATGACACTCATTCAAAAGGAATTCCTTTGTTCTGTTTTGATAGTTCTTATCAGTTGCCATTTGAAATATCGATTGATTCCGCCAATGATTTTCAAGTTCTATTTTTCTACTTAAAAATGGTTCATCTTCTTTCCTTTTTCCTGAAATTGATTATCGTTACTTAATTAATAGACATGGAAAGCAACTTTGACTGAAAGTCGTACAGCTTCTCATGTTTTATCACTGTAATTGTCAGCTTCTCGGCACACCATCCGAGTCGGATCTTGGTTTTATTCGAAATGAAGACTCGAAAAGATATCTTCGGCAGCTACCTCCACATCCTCGTCAGCCATTAGCAACGGTTTTCCCACATGTTCATCCGTTGGCAATTGATCTTGTGGATAAAATGTTGACGTTTGACCCAACAAAGAGAATTACTGGTAAACATTTATCGAATATCCATTATTTTTCTTTTGATGGTCTGGATGAATTAATTGCTTGTTAAGTACAATGTTAATGATAGTTTAGTGATTGGAATGTGTGGAGTACAATACTTTAAATTATGACCTCTTCATTTTGTTACAAAATTATTAGATTCGACGTGCAAACTATCCTTTTTGTTGGCCATGCTCATATAGATTGATTTCGATCAGATTATAATTCAACAATTGATGTTGAAATACTCTTTCTGTTTCATATCATTCCTTGATTTAACTTTATGATCAATTTGTCTTTGGAACAGTTGAAGAAGCGTTGGCACATCCATACCTAGAAAGATTGCATGACATAGCGGATGAGCCGGTTTGCCCAGAGCCATTCTCGTTCGAGTTTGAGCAACAATTCTTAGATGAAGAACAGATGAAGGAAATGATTTACAGAGAGGCATTGGCACTCAATCCAGAATTCGCATGACCATGAAAATGTACATGCTCTAAGGGTCGTCTCCTCGAGTAACGACGACTTTGCAAATACAGGATAACATACGACGAAAACAGCTCAGGTTGGTGTACAAAACGAAATCACATCAAATAAGCTCAGTATGTTGAATTGGCTAACTTATCTGTTTCATTCATCTATCAGCATCAAACCCCTGAGCAAGCTCTTATCAAGAATACCCCTCTTTTTTCTTTTTTTTTTTCCCACGGAAATTGTTAGTTTATTTTTTTGCCCCACGTTTGATCCTCTTTAAGTTTGTGTTTTGTTGTTTTGTTTGTTTTGAAAATGTTGAATCTATATTTTGTACAGGAAACCAAATTGAATGAAAGTGTTCTATTTATGTTGGTTGTATGTCTCCATCTTTTGAATTAGAGCTGTTGTTCTGTAATCTTTTTGTCAGCATTTTATGAACATGATGTGTTTGATTTTAGTACATATATTTTCTTGTTAAAGTATTGATTTGTAATAAAAAGTTAGGTGTGGCATCACATAAAGCTGATGTAGATTGTTAAAGGATATATCATATATGATATTTCCAAATAAATATAATGCTAAAGTGGGTCTATTTATACCTTA

mRNA sequence

AAGATGACGAAGGAAGAAGCCGTTGAAAAAACGAAAAACGAACACGAACCGTCGATCAAAGGCGGCGGAAATCTGAAACGTCCGATCTTCCGTCAGAAGCGCGTGAGTCATTTTTCTCCCGCGCACGAGCCTTCGTTTCCCTTTCCTCGCATTTACTCTTCACACTCTCTCCTTTTTCCCTTTGACAAATTCTTCTCTGCATAAATATTCATCCAAATTCTTCACTTCTTCTTCAAACTCTTGGTCTTGGTCTTCCTTCTTCATCTGGGGTTTCAATTTCTTGACTGAGAATCGCAGATTTTGATACAGAAGGTGTTTCAGGCAAAGGGTTTCGAAGATTATGGCTGATGTTGGTCAGAACAACCCCGCTGATTTTCCTGCTCTTTCAAGCCATGGTGGTCAATACGTTCAGTACAATATCTTTGGGAATCCTTTCGAAATCACCTCCAAATATCGTCCCCCTATTATGCCTATAGGTCGCGGCGCATACGGAATCGTTTGTTCTGTTTTGAATTCGGAGACCAATGAGATGGTTGCGGTTAAGAAGATTGCTAACGCGTTTGATAACCATATGGATGCGAAGAGGACGCTACGTGAGATTAAGCTTCTTCGCCATTTGGATCATGAAAATGTAATAGGCATAAGAGATGTGATTCCTCCACCCTTACGGAGAGAATTCAATGATGTTTACATTTCAACTGAACTAATGGATACCGATCTTCACCAAATAATCCGTTCCAACCAAGGTTTATCGGAAGAGCATTGTCAGTATTTCCTTTATCAGATTCTCCGAGGACTGAAATACATACATTCTGCCAATGTCATTCATCGAGACTTGAAACCGAGCAATCTATTGCTTAATGCAAACTGTGATCTTAAAATATGTGATTTTGGTCTTGCTCGACCAACTTCGGAAAATGAAAGTATGACAGAATATGTTGTGACAAGATGGTATAGAGCACCTGAGCTTCTATTGAACTCTGATTATACAGCTGCTATAGATATATGGTCTGTTGGTTGTATCTTTCTGGAGCTTATGAATAGAAGGCCTTTATTTCCAGGCAGGGATCATGTGCATCAGATGCGATTATTGACTGAGCTTCTCGGCACACCATCCGAGTCGGATCTTGGTTTTATTCGAAATGAAGACTCGAAAAGATATCTTCGGCAGCTACCTCCACATCCTCGTCAGCCATTAGCAACGGTTTTCCCACATGTTCATCCGTTGGCAATTGATCTTGTGGATAAAATGTTGACGTTTGACCCAACAAAGAGAATTACTGTTGAAGAAGCGTTGGCACATCCATACCTAGAAAGATTGCATGACATAGCGGATGAGCCGGTTTGCCCAGAGCCATTCTCGTTCGAGTTTGAGCAACAATTCTTAGATGAAGAACAGATGAAGGAAATGATTTACAGAGAGGCATTGGCACTCAATCCAGAATTCGCATGACCATGAAAATGTACATGCTCTAAGGGTCGTCTCCTCGAGTAACGACGACTTTGCAAATACAGGATAACATACGACGAAAACAGCTCAGGTTGGTGTACAAAACGAAATCACATCAAATAAGCTCAGTATGTTGAATTGGCTAACTTATCTGTTTCATTCATCTATCAGCATCAAACCCCTGAGCAAGCTCTTATCAAGAATACCCCTCTTTTTTCTTTTTTTTTTTCCCACGGAAATTGTTAGTTTATTTTTTTGCCCCACGTTTGATCCTCTTTAAGTTTGTGTTTTGTTGTTTTGTTTGTTTTGAAAATGTTGAATCTATATTTTGTACAGGAAACCAAATTGAATGAAAGTGTTCTATTTATGTTGGTTGTATGTCTCCATCTTTTGAATTAGAGCTGTTGTTCTGTAATCTTTTTGTCAGCATTTTATGAACATGATGTGTTTGATTTTAGTACATATATTTTCTTGTTAAAGTATTGATTTGTAATAAAAAGTTAGGTGTGGCATCACATAAAGCTGATGTAGATTGTTAAAGGATATATCATATATGATATTTCCAAATAAATATAATGCTAAAGTGGGTCTATTTATACCTTA

Coding sequence (CDS)

ATGGCTGATGTTGGTCAGAACAACCCCGCTGATTTTCCTGCTCTTTCAAGCCATGGTGGTCAATACGTTCAGTACAATATCTTTGGGAATCCTTTCGAAATCACCTCCAAATATCGTCCCCCTATTATGCCTATAGGTCGCGGCGCATACGGAATCGTTTGTTCTGTTTTGAATTCGGAGACCAATGAGATGGTTGCGGTTAAGAAGATTGCTAACGCGTTTGATAACCATATGGATGCGAAGAGGACGCTACGTGAGATTAAGCTTCTTCGCCATTTGGATCATGAAAATGTAATAGGCATAAGAGATGTGATTCCTCCACCCTTACGGAGAGAATTCAATGATGTTTACATTTCAACTGAACTAATGGATACCGATCTTCACCAAATAATCCGTTCCAACCAAGGTTTATCGGAAGAGCATTGTCAGTATTTCCTTTATCAGATTCTCCGAGGACTGAAATACATACATTCTGCCAATGTCATTCATCGAGACTTGAAACCGAGCAATCTATTGCTTAATGCAAACTGTGATCTTAAAATATGTGATTTTGGTCTTGCTCGACCAACTTCGGAAAATGAAAGTATGACAGAATATGTTGTGACAAGATGGTATAGAGCACCTGAGCTTCTATTGAACTCTGATTATACAGCTGCTATAGATATATGGTCTGTTGGTTGTATCTTTCTGGAGCTTATGAATAGAAGGCCTTTATTTCCAGGCAGGGATCATGTGCATCAGATGCGATTATTGACTGAGCTTCTCGGCACACCATCCGAGTCGGATCTTGGTTTTATTCGAAATGAAGACTCGAAAAGATATCTTCGGCAGCTACCTCCACATCCTCGTCAGCCATTAGCAACGGTTTTCCCACATGTTCATCCGTTGGCAATTGATCTTGTGGATAAAATGTTGACGTTTGACCCAACAAAGAGAATTACTGTTGAAGAAGCGTTGGCACATCCATACCTAGAAAGATTGCATGACATAGCGGATGAGCCGGTTTGCCCAGAGCCATTCTCGTTCGAGTTTGAGCAACAATTCTTAGATGAAGAACAGATGAAGGAAATGATTTACAGAGAGGCATTGGCACTCAATCCAGAATTCGCATGA

Protein sequence

MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFPGRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYREALALNPEFA
Homology
BLAST of Lsi02G014060 vs. ExPASy Swiss-Prot
Match: Q39023 (Mitogen-activated protein kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MPK3 PE=1 SV=2)

HSP 1 Score: 640.2 bits (1650), Expect = 1.5e-182
Identity = 301/368 (81.79%), Postives = 340/368 (92.39%), Query Frame = 0

Query: 3   DVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETN 62
           + G     DFPA+ +HGGQ++ Y+IFG+ FEITSKYRPPI+PIGRGAYGIVCSVL++ETN
Sbjct: 2   NTGGGQYTDFPAVETHGGQFISYDIFGSLFEITSKYRPPIIPIGRGAYGIVCSVLDTETN 61

Query: 63  EMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTEL 122
           E+VA+KKIANAFDNHMDAKRTLREIKLLRHLDHEN+I IRDV+PPPLRR+F+DVYISTEL
Sbjct: 62  ELVAMKKIANAFDNHMDAKRTLREIKLLRHLDHENIIAIRDVVPPPLRRQFSDVYISTEL 121

Query: 123 MDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKIC 182
           MDTDLHQIIRSNQ LSEEHCQYFLYQ+LRGLKYIHSAN+IHRDLKPSNLLLNANCDLKIC
Sbjct: 122 MDTDLHQIIRSNQSLSEEHCQYFLYQLLRGLKYIHSANIIHRDLKPSNLLLNANCDLKIC 181

Query: 183 DFGLARPTSENESMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPG 242
           DFGLARPTSEN+ MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELMNR+PLFPG
Sbjct: 182 DFGLARPTSENDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMNRKPLFPG 241

Query: 243 RDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLV 302
           +DHVHQMRLLTELLGTP+ESDLGF  NED+KRY+RQLP  PRQPLA +F HV+P+AIDLV
Sbjct: 242 KDHVHQMRLLTELLGTPTESDLGFTHNEDAKRYIRQLPNFPRQPLAKLFSHVNPMAIDLV 301

Query: 303 DKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYRE 362
           D+MLTFDP +RITVE+AL H YL +LHD  DEP+C +PFSFEFEQQ LDEEQ+KEMIY+E
Sbjct: 302 DRMLTFDPNRRITVEQALNHQYLAKLHDPNDEPICQKPFSFEFEQQPLDEEQIKEMIYQE 361

Query: 363 ALALNPEF 370
           A+ALNP +
Sbjct: 362 AIALNPTY 369

BLAST of Lsi02G014060 vs. ExPASy Swiss-Prot
Match: Q07176 (Mitogen-activated protein kinase homolog MMK1 OS=Medicago sativa OX=3879 GN=MMK1 PE=1 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 2.7e-173
Identity = 290/360 (80.56%), Postives = 324/360 (90.00%), Query Frame = 0

Query: 11  DFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETNEMVAVKKI 70
           + PA+ SHGG+++QYNIFGN FE+T+KY+PPIMPIG+GAYGIVCS  NSETNE VAVKKI
Sbjct: 27  NIPAVLSHGGRFIQYNIFGNIFEVTAKYKPPIMPIGKGAYGIVCSAHNSETNEHVAVKKI 86

Query: 71  ANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMDTDLHQI 130
           ANAFDN +DAKRTLREIKLLRH+DHENV+ IRD++PPP R  FNDVYI+ ELMDTDLHQI
Sbjct: 87  ANAFDNKIDAKRTLREIKLLRHMDHENVVAIRDIVPPPQREVFNDVYIAYELMDTDLHQI 146

Query: 131 IRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARPT 190
           IRSNQ LSEEHCQYFLYQILRGLKYIHSANV+HRDLKPSNLLLNANCDLKICDFGLAR T
Sbjct: 147 IRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDFGLARVT 206

Query: 191 SENESMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRDHVHQMR 250
           SE + MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELM+R+PLFPGRDHVHQ+R
Sbjct: 207 SETDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQLR 266

Query: 251 LLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDKMLTFDP 310
           LL EL+GTPSE DLGF+ NE++KRY+RQLPP+ RQ     FPHVHP AIDLV+KMLTFDP
Sbjct: 267 LLMELIGTPSEDDLGFL-NENAKRYIRQLPPYRRQSFQEKFPHVHPEAIDLVEKMLTFDP 326

Query: 311 TKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYREALALNPEF 370
            KRITVE+ALAHPYL  LHDI+DEPVC  PFSF+FEQ  L EEQMKE+IYREALA NPE+
Sbjct: 327 RKRITVEDALAHPYLTSLHDISDEPVCMTPFSFDFEQHALTEEQMKELIYREALAFNPEY 385

BLAST of Lsi02G014060 vs. ExPASy Swiss-Prot
Match: Q06060 (Mitogen-activated protein kinase homolog D5 OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 2.0e-171
Identity = 288/360 (80.00%), Postives = 322/360 (89.44%), Query Frame = 0

Query: 11  DFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETNEMVAVKKI 70
           + PA  SHGG+++QYNIFGN FE+T+KYRPPIMPIG+GAYGIVCS  NSETNE VAVKKI
Sbjct: 34  NIPATLSHGGRFIQYNIFGNIFEVTAKYRPPIMPIGKGAYGIVCSAHNSETNEHVAVKKI 93

Query: 71  ANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMDTDLHQI 130
           ANAFDN +DAKRTLREIKL+RH+DHENV+ IRD++PPP R  FNDVYI+ ELMDTDLHQI
Sbjct: 94  ANAFDNKIDAKRTLREIKLVRHMDHENVVAIRDIVPPPQREVFNDVYIAYELMDTDLHQI 153

Query: 131 IRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARPT 190
           IRSNQ LSEEHCQYFLYQILRGLKYIHSANV+HRDLKPSNLLLNANCDLKICDFGLAR T
Sbjct: 154 IRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDFGLARVT 213

Query: 191 SENESMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRDHVHQMR 250
           SE + MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELM+R+PLFPGRDHVHQ+R
Sbjct: 214 SETDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQLR 273

Query: 251 LLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDKMLTFDP 310
           LL EL+GTPSE+DLGF+ NE++KRY+RQLP + RQ     FPHVHP AIDLV+KMLTFDP
Sbjct: 274 LLMELIGTPSEADLGFL-NENAKRYIRQLPLYRRQSFQEKFPHVHPEAIDLVEKMLTFDP 333

Query: 311 TKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYREALALNPEF 370
            +RITVE ALAHPYL  LHDI+DEPVC  PFSF+FEQ  L EEQMKE+IYREALA NPE+
Sbjct: 334 RQRITVENALAHPYLTSLHDISDEPVCTTPFSFDFEQHALTEEQMKELIYREALAFNPEY 392

BLAST of Lsi02G014060 vs. ExPASy Swiss-Prot
Match: Q39026 (Mitogen-activated protein kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MPK6 PE=1 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 2.6e-171
Identity = 283/360 (78.61%), Postives = 324/360 (90.00%), Query Frame = 0

Query: 11  DFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETNEMVAVKKI 70
           + PA  SHGG+++QYNIFGN FE+T+KY+PPIMPIG+GAYGIVCS +NSETNE VA+KKI
Sbjct: 35  NIPATLSHGGRFIQYNIFGNIFEVTAKYKPPIMPIGKGAYGIVCSAMNSETNESVAIKKI 94

Query: 71  ANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMDTDLHQI 130
           ANAFDN +DAKRTLREIKLLRH+DHEN++ IRD+IPPPLR  FNDVYI+ ELMDTDLHQI
Sbjct: 95  ANAFDNKIDAKRTLREIKLLRHMDHENIVAIRDIIPPPLRNAFNDVYIAYELMDTDLHQI 154

Query: 131 IRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARPT 190
           IRSNQ LSEEHCQYFLYQILRGLKYIHSANV+HRDLKPSNLLLNANCDLKICDFGLAR T
Sbjct: 155 IRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDFGLARVT 214

Query: 191 SENESMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRDHVHQMR 250
           SE++ MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELM+R+PLFPGRDHVHQ+R
Sbjct: 215 SESDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQLR 274

Query: 251 LLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDKMLTFDP 310
           LL EL+GTPSE +L F+ NE++KRY+RQLPP+PRQ +   FP VHPLAIDL++KMLTFDP
Sbjct: 275 LLMELIGTPSEEELEFL-NENAKRYIRQLPPYPRQSITDKFPTVHPLAIDLIEKMLTFDP 334

Query: 311 TKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYREALALNPEF 370
            +RITV +ALAHPYL  LHDI+DEP C  PF+F+FE   L EEQMKE+IYREALA NPE+
Sbjct: 335 RRRITVLDALAHPYLNSLHDISDEPECTIPFNFDFENHALSEEQMKELIYREALAFNPEY 393

BLAST of Lsi02G014060 vs. ExPASy Swiss-Prot
Match: A2XFC8 (Mitogen-activated protein kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=MPK5 PE=1 SV=2)

HSP 1 Score: 600.5 bits (1547), Expect = 1.3e-170
Identity = 276/359 (76.88%), Postives = 330/359 (91.92%), Query Frame = 0

Query: 10  ADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETNEMVAVKK 69
           A+F    +HGG+Y+ Y+IFGN FE+T+KY+PPIMPIGRGAYGIVCSV+N ET EMVA+KK
Sbjct: 7   AEFRPTMTHGGRYLLYDIFGNKFEVTNKYQPPIMPIGRGAYGIVCSVMNFETREMVAIKK 66

Query: 70  IANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMDTDLHQ 129
           IANAF+N MDAKRTLREIKLLRHLDHEN+IGIRDVIPPP+ + FNDVYI+TELMDTDLH 
Sbjct: 67  IANAFNNDMDAKRTLREIKLLRHLDHENIIGIRDVIPPPIPQAFNDVYIATELMDTDLHH 126

Query: 130 IIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARP 189
           IIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARP
Sbjct: 127 IIRSNQELSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARP 186

Query: 190 TSENESMTEYVVTRWYRAPELLLNS-DYTAAIDIWSVGCIFLELMNRRPLFPGRDHVHQM 249
           +SE++ MTEYVVTRWYRAPELLLNS DY+AAID+WSVGCIF+EL+NR+PLFPGRDH+HQM
Sbjct: 187 SSESDMMTEYVVTRWYRAPELLLNSTDYSAAIDVWSVGCIFMELINRQPLFPGRDHMHQM 246

Query: 250 RLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDKMLTFD 309
           RL+TE++GTP++ +LGFIRNED+++Y+R LP +PR+  A++FP V P A+DL+++MLTF+
Sbjct: 247 RLITEVIGTPTDDELGFIRNEDARKYMRHLPQYPRRTFASMFPRVQPAALDLIERMLTFN 306

Query: 310 PTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYREALALNP 368
           P +RITVEEAL HPYLERLHDIADEP+C EPFSF+FEQ+ L+E+QMK++I+ EA+ +NP
Sbjct: 307 PLQRITVEEALDHPYLERLHDIADEPICLEPFSFDFEQKALNEDQMKQLIFNEAIEMNP 365

BLAST of Lsi02G014060 vs. ExPASy TrEMBL
Match: Q0R4I2 (Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=TIPK PE=2 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 5.7e-214
Identity = 364/370 (98.38%), Postives = 366/370 (98.92%), Query Frame = 0

Query: 1   MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60
           MADVGQNNPADFPAL +HGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE
Sbjct: 1   MADVGQNNPADFPALPTHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60

Query: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120
           TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST
Sbjct: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120

Query: 121 ELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240
           ICDFGLARPTSENE MTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP
Sbjct: 181 ICDFGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240

Query: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300
           GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL
Sbjct: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300

Query: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYR 360
           VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVC EPFSFEFEQQ+LDEEQMKEMIYR
Sbjct: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYR 360

Query: 361 EALALNPEFA 371
           EALALNPEFA
Sbjct: 361 EALALNPEFA 370

BLAST of Lsi02G014060 vs. ExPASy TrEMBL
Match: F8ULD9 (Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103497219 PE=2 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 2.4e-212
Identity = 361/370 (97.57%), Postives = 364/370 (98.38%), Query Frame = 0

Query: 1   MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60
           MADVGQNNPADFPAL +HGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE
Sbjct: 1   MADVGQNNPADFPALPTHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60

Query: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120
           TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYI T
Sbjct: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYILT 120

Query: 121 ELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240
           ICDFGLARPTSENE MTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP
Sbjct: 181 ICDFGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240

Query: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300
           GRDHVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL
Sbjct: 241 GRDHVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300

Query: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYR 360
           VDKMLTFDPTKRITVEEALAHPYLERLHD ADEPVC EPFSFEFEQQ+LDEEQMKEMIYR
Sbjct: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDTADEPVCSEPFSFEFEQQYLDEEQMKEMIYR 360

Query: 361 EALALNPEFA 371
           EALALNPEFA
Sbjct: 361 EALALNPEFA 370

BLAST of Lsi02G014060 vs. ExPASy TrEMBL
Match: A0A6J1KFE1 (Mitogen-activated protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111492741 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 7.7e-211
Identity = 357/370 (96.49%), Postives = 365/370 (98.65%), Query Frame = 0

Query: 1   MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60
           MADVGQNN  DFPA+ +HGGQYVQYNIF NPFEITSKYRPPIMPIGRGAYGIVCSVLNSE
Sbjct: 1   MADVGQNNTGDFPAVPTHGGQYVQYNIFRNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60

Query: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120
           TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREF+DVYIST
Sbjct: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFSDVYIST 120

Query: 121 ELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSNQTLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240
           ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCI+LELMNRRPLFP
Sbjct: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIYLELMNRRPLFP 240

Query: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300
           GRDHVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL
Sbjct: 241 GRDHVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300

Query: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYR 360
           VDKMLTFDPT+RITVEEALAHPYLERLHD+ADEPVCPEPFSFEFEQQ+LDEEQMKEMIYR
Sbjct: 301 VDKMLTFDPTRRITVEEALAHPYLERLHDVADEPVCPEPFSFEFEQQYLDEEQMKEMIYR 360

Query: 361 EALALNPEFA 371
           EALALNPEFA
Sbjct: 361 EALALNPEFA 370

BLAST of Lsi02G014060 vs. ExPASy TrEMBL
Match: A0A6J1GAP2 (Mitogen-activated protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111452292 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 5.0e-210
Identity = 353/370 (95.41%), Postives = 365/370 (98.65%), Query Frame = 0

Query: 1   MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60
           MADVGQNN  DFPA+ +HGGQYVQYNIFGNPFEITSKYRPPIMP+GRGAYGIVCS+LNSE
Sbjct: 1   MADVGQNNTGDFPAVPTHGGQYVQYNIFGNPFEITSKYRPPIMPVGRGAYGIVCSILNSE 60

Query: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120
           TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPP+RREF+DVYIST
Sbjct: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPVRREFSDVYIST 120

Query: 121 ELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSNQTLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240
           ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCI+LELMNRRPLFP
Sbjct: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIYLELMNRRPLFP 240

Query: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300
           GRDHVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL
Sbjct: 241 GRDHVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300

Query: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYR 360
           VD+MLTFDPT+RITVEEALAHPYLERLHD+ADEPVCP PFSFEFEQQ+LDEEQMKEMIYR
Sbjct: 301 VDRMLTFDPTRRITVEEALAHPYLERLHDVADEPVCPVPFSFEFEQQYLDEEQMKEMIYR 360

Query: 361 EALALNPEFA 371
           EALALNPEFA
Sbjct: 361 EALALNPEFA 370

BLAST of Lsi02G014060 vs. ExPASy TrEMBL
Match: A0A6J1DYR6 (Mitogen-activated protein kinase OS=Momordica charantia OX=3673 GN=LOC111025488 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 7.2e-209
Identity = 355/371 (95.69%), Postives = 364/371 (98.11%), Query Frame = 0

Query: 1   MADVGQNN-PADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNS 60
           MADVG NN  ADFPA+ +HGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNS
Sbjct: 1   MADVGHNNAAADFPAVPTHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNS 60

Query: 61  ETNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIS 120
           ETNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYI+
Sbjct: 61  ETNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIA 120

Query: 121 TELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDL 180
           TELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDL
Sbjct: 121 TELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDL 180

Query: 181 KICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLF 240
           KICDFGLARPT+ENE MTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLF
Sbjct: 181 KICDFGLARPTAENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLF 240

Query: 241 PGRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAID 300
           PGRDHVHQMRLLTELLGTPSESDLGFIRNEDS+RYLRQLPPHPRQPLATVFPHVHPLAID
Sbjct: 241 PGRDHVHQMRLLTELLGTPSESDLGFIRNEDSRRYLRQLPPHPRQPLATVFPHVHPLAID 300

Query: 301 LVDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIY 360
           LVDKMLTF+PT RITVE ALAHPYLERLHDIADEPVCPEPFSFEFEQQF+DEEQMKEMIY
Sbjct: 301 LVDKMLTFNPTNRITVEAALAHPYLERLHDIADEPVCPEPFSFEFEQQFIDEEQMKEMIY 360

Query: 361 REALALNPEFA 371
           REA+ALNPE+A
Sbjct: 361 REAVALNPEYA 371

BLAST of Lsi02G014060 vs. NCBI nr
Match: XP_038895412.1 (mitogen-activated protein kinase 3 [Benincasa hispida])

HSP 1 Score: 755.7 bits (1950), Expect = 1.8e-214
Identity = 364/370 (98.38%), Postives = 368/370 (99.46%), Query Frame = 0

Query: 1   MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60
           MADVGQNNPADFPA+S+HGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE
Sbjct: 1   MADVGQNNPADFPAVSTHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60

Query: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120
           TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST
Sbjct: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120

Query: 121 ELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240
           ICDFGLARPTSENE MTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP
Sbjct: 181 ICDFGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240

Query: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300
           GRDHVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL
Sbjct: 241 GRDHVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300

Query: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYR 360
           VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQ+LDEEQMKEMIYR
Sbjct: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQYLDEEQMKEMIYR 360

Query: 361 EALALNPEFA 371
           EALALNPEFA
Sbjct: 361 EALALNPEFA 370

BLAST of Lsi02G014060 vs. NCBI nr
Match: NP_001267653.1 (mitogen-activated protein kinase 3-like [Cucumis sativus] >AAZ57337.1 Trichoderma-induced protein kinase [Cucumis sativus] >ABG89857.1 NO3-stress-induced mitogen activated protein kinase [Cucumis sativus] >KGN65657.1 hypothetical protein Csa_020125 [Cucumis sativus])

HSP 1 Score: 753.1 bits (1943), Expect = 1.2e-213
Identity = 364/370 (98.38%), Postives = 366/370 (98.92%), Query Frame = 0

Query: 1   MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60
           MADVGQNNPADFPAL +HGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE
Sbjct: 1   MADVGQNNPADFPALPTHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60

Query: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120
           TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST
Sbjct: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120

Query: 121 ELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240
           ICDFGLARPTSENE MTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP
Sbjct: 181 ICDFGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240

Query: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300
           GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL
Sbjct: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300

Query: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYR 360
           VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVC EPFSFEFEQQ+LDEEQMKEMIYR
Sbjct: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCSEPFSFEFEQQYLDEEQMKEMIYR 360

Query: 361 EALALNPEFA 371
           EALALNPEFA
Sbjct: 361 EALALNPEFA 370

BLAST of Lsi02G014060 vs. NCBI nr
Match: NP_001284376.1 (mitogen-activated protein kinase 3 [Cucumis melo] >AEG89601.1 mitogen-activated protein [Cucumis melo])

HSP 1 Score: 747.7 bits (1929), Expect = 4.9e-212
Identity = 361/370 (97.57%), Postives = 364/370 (98.38%), Query Frame = 0

Query: 1   MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60
           MADVGQNNPADFPAL +HGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE
Sbjct: 1   MADVGQNNPADFPALPTHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60

Query: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120
           TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYI T
Sbjct: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYILT 120

Query: 121 ELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSNQSLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240
           ICDFGLARPTSENE MTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP
Sbjct: 181 ICDFGLARPTSENECMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240

Query: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300
           GRDHVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL
Sbjct: 241 GRDHVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300

Query: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYR 360
           VDKMLTFDPTKRITVEEALAHPYLERLHD ADEPVC EPFSFEFEQQ+LDEEQMKEMIYR
Sbjct: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDTADEPVCSEPFSFEFEQQYLDEEQMKEMIYR 360

Query: 361 EALALNPEFA 371
           EALALNPEFA
Sbjct: 361 EALALNPEFA 370

BLAST of Lsi02G014060 vs. NCBI nr
Match: KAG6607201.1 (Mitogen-activated protein kinase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 742.7 bits (1916), Expect = 1.6e-210
Identity = 354/370 (95.68%), Postives = 366/370 (98.92%), Query Frame = 0

Query: 1   MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60
           MADVGQNN  DFPA+ +HGGQYVQYNIFGNPFEITSKYRPPIMP+GRGAYGIVCS+LNSE
Sbjct: 120 MADVGQNNTGDFPAVPTHGGQYVQYNIFGNPFEITSKYRPPIMPVGRGAYGIVCSILNSE 179

Query: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120
           TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPP+RREF+DVYIST
Sbjct: 180 TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPVRREFSDVYIST 239

Query: 121 ELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK
Sbjct: 240 ELMDTDLHQIIRSNQTLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 299

Query: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240
           ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCI+LELMNRRPLFP
Sbjct: 300 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIYLELMNRRPLFP 359

Query: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300
           GRDHVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL
Sbjct: 360 GRDHVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 419

Query: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYR 360
           VD+MLTFDPT+RITVEEALAHPYLERLHD+ADEPVCPEPFSFEFEQQ+LDEEQMKEMIYR
Sbjct: 420 VDRMLTFDPTRRITVEEALAHPYLERLHDVADEPVCPEPFSFEFEQQYLDEEQMKEMIYR 479

Query: 361 EALALNPEFA 371
           EALALNPEFA
Sbjct: 480 EALALNPEFA 489

BLAST of Lsi02G014060 vs. NCBI nr
Match: XP_022997943.1 (mitogen-activated protein kinase 3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 742.7 bits (1916), Expect = 1.6e-210
Identity = 357/370 (96.49%), Postives = 365/370 (98.65%), Query Frame = 0

Query: 1   MADVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60
           MADVGQNN  DFPA+ +HGGQYVQYNIF NPFEITSKYRPPIMPIGRGAYGIVCSVLNSE
Sbjct: 1   MADVGQNNTGDFPAVPTHGGQYVQYNIFRNPFEITSKYRPPIMPIGRGAYGIVCSVLNSE 60

Query: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYIST 120
           TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREF+DVYIST
Sbjct: 61  TNEMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFSDVYIST 120

Query: 121 ELMDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSNQ LSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSNQTLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIFLELMNRRPLFP 240
           ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCI+LELMNRRPLFP
Sbjct: 181 ICDFGLARPTSENESMTEYVVTRWYRAPELLLNSDYTAAIDIWSVGCIYLELMNRRPLFP 240

Query: 241 GRDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300
           GRDHVHQMRLLTELLGTP+ESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL
Sbjct: 241 GRDHVHQMRLLTELLGTPTESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDL 300

Query: 301 VDKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYR 360
           VDKMLTFDPT+RITVEEALAHPYLERLHD+ADEPVCPEPFSFEFEQQ+LDEEQMKEMIYR
Sbjct: 301 VDKMLTFDPTRRITVEEALAHPYLERLHDVADEPVCPEPFSFEFEQQYLDEEQMKEMIYR 360

Query: 361 EALALNPEFA 371
           EALALNPEFA
Sbjct: 361 EALALNPEFA 370

BLAST of Lsi02G014060 vs. TAIR 10
Match: AT3G45640.1 (mitogen-activated protein kinase 3 )

HSP 1 Score: 640.2 bits (1650), Expect = 1.0e-183
Identity = 301/368 (81.79%), Postives = 340/368 (92.39%), Query Frame = 0

Query: 3   DVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETN 62
           + G     DFPA+ +HGGQ++ Y+IFG+ FEITSKYRPPI+PIGRGAYGIVCSVL++ETN
Sbjct: 2   NTGGGQYTDFPAVETHGGQFISYDIFGSLFEITSKYRPPIIPIGRGAYGIVCSVLDTETN 61

Query: 63  EMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTEL 122
           E+VA+KKIANAFDNHMDAKRTLREIKLLRHLDHEN+I IRDV+PPPLRR+F+DVYISTEL
Sbjct: 62  ELVAMKKIANAFDNHMDAKRTLREIKLLRHLDHENIIAIRDVVPPPLRRQFSDVYISTEL 121

Query: 123 MDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKIC 182
           MDTDLHQIIRSNQ LSEEHCQYFLYQ+LRGLKYIHSAN+IHRDLKPSNLLLNANCDLKIC
Sbjct: 122 MDTDLHQIIRSNQSLSEEHCQYFLYQLLRGLKYIHSANIIHRDLKPSNLLLNANCDLKIC 181

Query: 183 DFGLARPTSENESMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPG 242
           DFGLARPTSEN+ MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELMNR+PLFPG
Sbjct: 182 DFGLARPTSENDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMNRKPLFPG 241

Query: 243 RDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLV 302
           +DHVHQMRLLTELLGTP+ESDLGF  NED+KRY+RQLP  PRQPLA +F HV+P+AIDLV
Sbjct: 242 KDHVHQMRLLTELLGTPTESDLGFTHNEDAKRYIRQLPNFPRQPLAKLFSHVNPMAIDLV 301

Query: 303 DKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYRE 362
           D+MLTFDP +RITVE+AL H YL +LHD  DEP+C +PFSFEFEQQ LDEEQ+KEMIY+E
Sbjct: 302 DRMLTFDPNRRITVEQALNHQYLAKLHDPNDEPICQKPFSFEFEQQPLDEEQIKEMIYQE 361

Query: 363 ALALNPEF 370
           A+ALNP +
Sbjct: 362 AIALNPTY 369

BLAST of Lsi02G014060 vs. TAIR 10
Match: AT2G43790.1 (MAP kinase 6 )

HSP 1 Score: 602.8 bits (1553), Expect = 1.8e-172
Identity = 283/360 (78.61%), Postives = 324/360 (90.00%), Query Frame = 0

Query: 11  DFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETNEMVAVKKI 70
           + PA  SHGG+++QYNIFGN FE+T+KY+PPIMPIG+GAYGIVCS +NSETNE VA+KKI
Sbjct: 35  NIPATLSHGGRFIQYNIFGNIFEVTAKYKPPIMPIGKGAYGIVCSAMNSETNESVAIKKI 94

Query: 71  ANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMDTDLHQI 130
           ANAFDN +DAKRTLREIKLLRH+DHEN++ IRD+IPPPLR  FNDVYI+ ELMDTDLHQI
Sbjct: 95  ANAFDNKIDAKRTLREIKLLRHMDHENIVAIRDIIPPPLRNAFNDVYIAYELMDTDLHQI 154

Query: 131 IRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARPT 190
           IRSNQ LSEEHCQYFLYQILRGLKYIHSANV+HRDLKPSNLLLNANCDLKICDFGLAR T
Sbjct: 155 IRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDFGLARVT 214

Query: 191 SENESMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRDHVHQMR 250
           SE++ MTEYVVTRWYRAPELLLN SDYTAAID+WSVGCIF+ELM+R+PLFPGRDHVHQ+R
Sbjct: 215 SESDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQLR 274

Query: 251 LLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDKMLTFDP 310
           LL EL+GTPSE +L F+ NE++KRY+RQLPP+PRQ +   FP VHPLAIDL++KMLTFDP
Sbjct: 275 LLMELIGTPSEEELEFL-NENAKRYIRQLPPYPRQSITDKFPTVHPLAIDLIEKMLTFDP 334

Query: 311 TKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYREALALNPEF 370
            +RITV +ALAHPYL  LHDI+DEP C  PF+F+FE   L EEQMKE+IYREALA NPE+
Sbjct: 335 RRRITVLDALAHPYLNSLHDISDEPECTIPFNFDFENHALSEEQMKELIYREALAFNPEY 393

BLAST of Lsi02G014060 vs. TAIR 10
Match: AT4G01370.1 (MAP kinase 4 )

HSP 1 Score: 529.3 bits (1362), Expect = 2.6e-150
Identity = 244/355 (68.73%), Postives = 301/355 (84.79%), Query Frame = 0

Query: 15  LSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETNEMVAVKKIANAF 74
           +++HGG YVQYN++GN FE++ KY PP+ PIGRGAYGIVC+  NSET E VA+KKI NAF
Sbjct: 19  VATHGGSYVQYNVYGNLFEVSRKYVPPLRPIGRGAYGIVCAATNSETGEEVAIKKIGNAF 78

Query: 75  DNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMDTDLHQIIRSN 134
           DN +DAKRTLREIKLL+H+DHENVI ++D+I PP R  FNDVYI  ELMDTDLHQIIRSN
Sbjct: 79  DNIIDAKRTLREIKLLKHMDHENVIAVKDIIKPPQRENFNDVYIVYELMDTDLHQIIRSN 138

Query: 135 QGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARPTSENE 194
           Q L+++HC++FLYQ+LRGLKY+HSANV+HRDLKPSNLLLNANCDLK+ DFGLAR  SE +
Sbjct: 139 QPLTDDHCRFFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKLGDFGLARTKSETD 198

Query: 195 SMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPGRDHVHQMRLLTE 254
            MTEYVVTRWYRAPELLLN S+YTAAIDIWSVGCI  E M R PLFPG+D+VHQ+RL+TE
Sbjct: 199 FMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGETMTREPLFPGKDYVHQLRLITE 258

Query: 255 LLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDKMLTFDPTKRI 314
           L+G+P +S LGF+R+++++RY+RQLP +PRQ  A  FP++   A+DL++KML FDP++RI
Sbjct: 259 LIGSPDDSSLGFLRSDNARRYVRQLPQYPRQNFAARFPNMSAGAVDLLEKMLVFDPSRRI 318

Query: 315 TVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYREALALNPE 369
           TV+EAL HPYL  LHDI +EPVC  PF+F+FEQ  L EE +KE+IYRE +  NP+
Sbjct: 319 TVDEALCHPYLAPLHDINEEPVCVRPFNFDFEQPTLTEENIKELIYRETVKFNPQ 373

BLAST of Lsi02G014060 vs. TAIR 10
Match: AT3G59790.1 (MAP kinase 10 )

HSP 1 Score: 508.4 bits (1308), Expect = 4.7e-144
Identity = 242/359 (67.41%), Postives = 293/359 (81.62%), Query Frame = 0

Query: 11  DFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETNEMVAVKKI 70
           D P   SH G+Y+QYN+FG+ FE+ +KY+PPI PIGRGA GIVCS ++SETNE VA+KKI
Sbjct: 32  DIPGTLSHDGRYIQYNLFGHIFELPAKYKPPIRPIGRGACGIVCSAVDSETNEKVAIKKI 91

Query: 71  ANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTELMDTDLHQI 130
              FDN ++AKRTLREIKLLRH DHEN++ IRDVI PP R  F DVYI  ELM+ DL++ 
Sbjct: 92  TQVFDNTIEAKRTLREIKLLRHFDHENIVAIRDVILPPQRDSFEDVYIVNELMEFDLYRT 151

Query: 131 IRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKICDFGLARPT 190
           ++S+Q L+++H  YF+YQILRGLKYIHSANV+HRDLKPSNLLL+  CDLKICDFGLAR T
Sbjct: 152 LKSDQELTKDHGMYFMYQILRGLKYIHSANVLHRDLKPSNLLLSTQCDLKICDFGLARAT 211

Query: 191 SENESMTEYVVTRWYRAPELLL-NSDYTAAIDIWSVGCIFLELMNRRPLFPGRDHVHQMR 250
            E+  MTEYVVTRWYRAPELLL +SDYTAAID+WSVGCIF+E+MNR PLFPG+D V+Q+R
Sbjct: 212 PESNLMTEYVVTRWYRAPELLLGSSDYTAAIDVWSVGCIFMEIMNREPLFPGKDQVNQLR 271

Query: 251 LLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLVDKMLTFDP 310
           LL EL+GTPSE +LG + +E +KRY+RQLP  PRQ     FP+V PLAIDLV+KMLTFDP
Sbjct: 272 LLLELIGTPSEEELGSL-SEYAKRYIRQLPTLPRQSFTEKFPNVPPLAIDLVEKMLTFDP 331

Query: 311 TKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYREALALNPE 369
            +RI+V+EALAHPYL   HDI DEP C EPF+F+ ++    EEQ +E+IY EALA NPE
Sbjct: 332 KQRISVKEALAHPYLSSFHDITDEPECSEPFNFDLDEHPFSEEQFRELIYCEALAFNPE 389

BLAST of Lsi02G014060 vs. TAIR 10
Match: AT4G11330.1 (MAP kinase 5 )

HSP 1 Score: 508.4 bits (1308), Expect = 4.7e-144
Identity = 237/366 (64.75%), Postives = 298/366 (81.42%), Query Frame = 0

Query: 3   DVGQNNPADFPALSSHGGQYVQYNIFGNPFEITSKYRPPIMPIGRGAYGIVCSVLNSETN 62
           D+G  N      +  HGG+Y QYN++GN FE+++KY PPI PIGRGAYG VC+ ++SET+
Sbjct: 10  DLGDTN---IKGVLVHGGRYFQYNVYGNLFEVSNKYVPPIRPIGRGAYGFVCAAVDSETH 69

Query: 63  EMVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGIRDVIPPPLRREFNDVYISTEL 122
           E +A+KKI  AFDN +DAKRTLREIKLLRHL+HENV+ I+D+I PP + +F DVYI  EL
Sbjct: 70  EEIAIKKIGKAFDNKVDAKRTLREIKLLRHLEHENVVVIKDIIRPPKKEDFVDVYIVFEL 129

Query: 123 MDTDLHQIIRSNQGLSEEHCQYFLYQILRGLKYIHSANVIHRDLKPSNLLLNANCDLKIC 182
           MDTDLHQIIRSNQ L+++HCQYFLYQILRGLKYIHSANV+HRDLKPSNLLLN+NCDLKI 
Sbjct: 130 MDTDLHQIIRSNQSLNDDHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNSNCDLKIT 189

Query: 183 DFGLARPTSENESMTEYVVTRWYRAPELLLN-SDYTAAIDIWSVGCIFLELMNRRPLFPG 242
           DFGLAR TSE E MTEYVVTRWYRAPELLLN S+YT+AID+WSVGCIF E+M R PLFPG
Sbjct: 190 DFGLARTTSETEYMTEYVVTRWYRAPELLLNSSEYTSAIDVWSVGCIFAEIMTREPLFPG 249

Query: 243 RDHVHQMRLLTELLGTPSESDLGFIRNEDSKRYLRQLPPHPRQPLATVFPHVHPLAIDLV 302
           +D+VHQ++L+TEL+G+P  + L F+R+ ++++Y+++LP  PRQ  +  FP ++  AIDL+
Sbjct: 250 KDYVHQLKLITELIGSPDGASLEFLRSANARKYVKELPKFPRQNFSARFPSMNSTAIDLL 309

Query: 303 DKMLTFDPTKRITVEEALAHPYLERLHDIADEPVCPEPFSFEFEQQFLDEEQMKEMIYRE 362
           +KML FDP KRITVEEAL +PYL  LHD+ DEPVC   FSF FE     EE++KE+++ E
Sbjct: 310 EKMLVFDPVKRITVEEALCYPYLSALHDLNDEPVCSNHFSFHFEDPSSTEEEIKELVWLE 369

Query: 363 ALALNP 368
           ++  NP
Sbjct: 370 SVKFNP 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q390231.5e-18281.79Mitogen-activated protein kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MPK3 PE=1 ... [more]
Q071762.7e-17380.56Mitogen-activated protein kinase homolog MMK1 OS=Medicago sativa OX=3879 GN=MMK1... [more]
Q060602.0e-17180.00Mitogen-activated protein kinase homolog D5 OS=Pisum sativum OX=3888 PE=2 SV=1[more]
Q390262.6e-17178.61Mitogen-activated protein kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MPK6 PE=1 ... [more]
A2XFC81.3e-17076.88Mitogen-activated protein kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=MPK... [more]
Match NameE-valueIdentityDescription
Q0R4I25.7e-21498.38Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=TIPK PE=2 SV=1[more]
F8ULD92.4e-21297.57Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103497219 PE=2 SV... [more]
A0A6J1KFE17.7e-21196.49Mitogen-activated protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111492741 PE=... [more]
A0A6J1GAP25.0e-21095.41Mitogen-activated protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111452292 P... [more]
A0A6J1DYR67.2e-20995.69Mitogen-activated protein kinase OS=Momordica charantia OX=3673 GN=LOC111025488 ... [more]
Match NameE-valueIdentityDescription
XP_038895412.11.8e-21498.38mitogen-activated protein kinase 3 [Benincasa hispida][more]
NP_001267653.11.2e-21398.38mitogen-activated protein kinase 3-like [Cucumis sativus] >AAZ57337.1 Trichoderm... [more]
NP_001284376.14.9e-21297.57mitogen-activated protein kinase 3 [Cucumis melo] >AEG89601.1 mitogen-activated ... [more]
KAG6607201.11.6e-21095.68Mitogen-activated protein kinase 3, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022997943.11.6e-21096.49mitogen-activated protein kinase 3-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G45640.11.0e-18381.79mitogen-activated protein kinase 3 [more]
AT2G43790.11.8e-17278.61MAP kinase 6 [more]
AT4G01370.12.6e-15068.73MAP kinase 4 [more]
AT3G59790.14.7e-14467.41MAP kinase 10 [more]
AT4G11330.14.7e-14464.75MAP kinase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 39..324
e-value: 3.9E-95
score: 332.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 44..324
e-value: 4.0E-74
score: 249.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 39..324
score: 45.474037
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 123..351
e-value: 9.7E-161
score: 536.0
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 22..361
e-value: 9.7E-161
score: 536.0
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 44..238
e-value: 2.0E-10
score: 38.3
NoneNo IPR availablePANTHERPTHR24055:SF502MITOGEN-ACTIVATED PROTEIN KINASEcoord: 15..369
NoneNo IPR availablePANTHERPTHR24055MITOGEN-ACTIVATED PROTEIN KINASEcoord: 15..369
NoneNo IPR availableCDDcd07858STKc_TEY_MAPKcoord: 32..367
e-value: 0.0
score: 669.848
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 45..69
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 161..173
IPR003527Mitogen-activated protein (MAP) kinase, conserved sitePROSITEPS01351MAPKcoord: 74..177
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 44..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G014060.1Lsi02G014060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010120 camalexin biosynthetic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0009611 response to wounding
biological_process GO:0009414 response to water deprivation
biological_process GO:0010224 response to UV-B
biological_process GO:0006979 response to oxidative stress
biological_process GO:1902065 response to L-glutamate
biological_process GO:0050826 response to freezing
biological_process GO:0042742 defense response to bacterium
biological_process GO:0010200 response to chitin
biological_process GO:0080136 priming of cellular response to stress
biological_process GO:1901002 positive regulation of response to salt stress
biological_process GO:0010183 pollen tube guidance
biological_process GO:0009875 pollen-pistil interaction
biological_process GO:0009555 pollen development
biological_process GO:0048481 plant ovule development
biological_process GO:0000165 MAPK cascade
biological_process GO:0010229 inflorescence development
biological_process GO:0050832 defense response to fungus
cellular_component GO:0005938 cell cortex
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0004707 MAP kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity