Lsi02G013430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G013430
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	abscisic acid 8'-hydroxylase 2
Location	chr02: 17923191 .. 17930996 (-)
RNA-Seq Expression	Lsi02G013430
Synteny	Lsi02G013430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTTGCATACAAATTTTGCTCTCCATAAATTAAATCTTTCTTTCTTTCTCCATTTCCACACAAAATAATAATAATAATGCTTCATCTTCTCTGTTTATTTGGTCTCTTTGTCTTTCTCTTATGTCTTCAATGGCGCCAACCCAAACACAAACTTCTTCCTCCTGGCTCTATGGGTTGGCCTTACATCGGCGAAACTTTTAAACTCTACACCCAAAATCCAAACTCGTTCTTCTCCATTAGGCAAAAAAGGTAACCCTAATTTCGAACAAAATTTTACCTTCTAATATTTTATATATAATTCTTCGATCTTAAAACTACGAGATTATATACATATATACTTTTTGAATTACGCCATACTACGAGGAAGACACAATTGTGTCTTCGCTCTACTCCTGGTTTTGATTGTTGTTGTTGTTATTGTTTTGTTGTATAGATATGGAGATGTTTTTAAGACACATATATTGGGTTGTCCTTGTGTAATGATTTCAAGTCCAAAGGCTGCAAGAGTTGTGCTTGTGAGTAAAGCTCATCTATTCAAGCCAACTTATCCACCAAGCAAAGAGAAAATGATAGGTCCTCAAGCTTTGTTTTTCCATCAAGGTCCTTACCATTCTCACCTTAAGAAGCTCATTCAAGCTTCCTTTTTGCCATCTGCTATAAAAGCCTCTATCTCTCAAATTGAGAACATTGTTCTCCACCTTCTCCCCTCTTGGAATAACTCCCAAATCAACACCTTGCAACAAATGAAAAGGGTAATTATTTCCTTCTTCTTTTCTTTTCTAATGGTTTTTTCTTCTGCTGTCCACCGCTGTTGGACTATGTTAATTGAACTATGTTCGTGTTAGAAACGGATAAATATCCATTCGGGAGAAGAAGGTTTGCAATGATATGACAGATTTTTGCCACATGTTAAGATGATTGAACTAAATTAGCATAGGGGGAAATTAAAATTGAATAATAATGAAGTGAAGTGTGTTGTTTTAATTACCATATTTGTTTGTTGCAGTTTGCTTTTGATGTGGCAATGATATCAGCTTTTGGAGACCAACAAGATTTGGAAATTGAAAGAATCAAACATTTGTACCAATGCCTTGAAAAAGGCTACAATTCTATGCCTCTTGATCTTCCTGGAACTCCTTTCCGCAAAGCAATGAAGGTATACCAAACAAACCTCCAATCAAAATATGTCCTGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATGTTATAAAAATACATGCGTTATTTTTCTAATATAGTATGAGAGCCATTAAAATCTAAATAGATAGTTAGTTAGAAAAAAAATTTAAAAAAATTAAAAAATATATAAATTACTCCTCTTATTTTATTCAAAACAAATTGAGATGGCCCATAAAAAAGGGAGATTAATGTTGAAATGGGTATTATTTCATTAGGCGAGGAAGGTGCTAAGTGAGACATTGGGGAAATTGATAGAGAAGAGACGGCGGAGCAAAGAGCATGGAGGTGGGTTGCTGGCGGTGCTACTGAGCGGTGGAGGTGGCGACGGTGGAGAGGATGAGAAAAAGAAGCTGAGTGATTCACAAATAGCAGATAATATAATAGGAGTAATATTTGCAGCACAAGACACGACAGCCAGTGTTTTAACATGGATTCTCAAGTATTTACACGACAATCACCATCTTCTTCAAGCTGTTCAGGTAAAATTAAATTTTACCTTTTTATTATTATTATATTTAATATTGGGTTAATAAAATTAAAAATTTGAAAAAAAAAATTATTCAGAAGGAGCAAGAGGCAATTTTTGAAAGGAAAGTGTGCGAAGGGAACCGTGGTCTTTCGTGGGATGATACAAGACGCATGCCCTTCACTAGTCGGGTATATTATATTATATTGTAGAAAAAATTTTATATATAAAAAAAATGTCAAACTATTTAAAGAAAATAGCAAAAAAAAAAAATACTGATAGAAATTGATAGACTTCTATCAATATATATCAGTGATAGACTTCTATTATTTTTATCACTAATAGATCCTGATAGACTTCTATCATTTTTATTACTGATAGATCCTGATAGATTTCTATTAGCGTTTATCACAACTATCTTGTGTAAATAATTTCTCCTATTTTTCTATTTTTAAAAATTTTTATATATTATATATAGATTTTTTTTAAATTTAATTTGAGATATGAAATTTTAAGGAATGGATTAAATAGTATTTAAGGTTTGGTATTAATTAATTAATTATGAAATTGATAGGTGATCCTAGAAACCCTAAGAAGAGCAAGTGTTGTGTCATTCACATTCAGGGAAGCAGTAGAAGAGGTTGAATTTGAAGGTTATTTAATCCCAAAAGGATGGAAAGTTCTTCCACTCTTCAGAACCATTCATCACTCCCCTGATTTCTTCCCACATCCCCACAACTTTGATCCTTCAAGATTCGAGGTGCCTATAATTTTATTATTATTATTTTTTTAAGACGGTTTTTAAATATAGAAAAATGAGTCAAATTATTTATAAATATAGAAAAAATTTACTGTCTATCGGCGATAGATTGCGATAGACAATGATAGAAGTCTATTGTTTAAAAGATAGAATTATATCGCAATCTAGCTGATAAACGACAGTGAAATTTTTCTATATTTATAAATAGTTTGATATTTTTTTTATAGTAATTTTTTTTCTTTTTAAATTTACTTTATATATATATAAGAAAATTTGTTTTTGTTTTTTTGTATTCAAATTTTGTTGAAGAGATGTATTTGGTCTTTGTGTTTAAAATTTTGTCTTTTTAAGTCTTTGAATTTTTAAGAATAGAGATGTGTTTTGTTCCTAAATAGATGTTTATTTATTTCATTTTTGTCCCTATATTTTTAATTTTGGTTCATTTTGATTCTTATACTTTTAAAATGTTCATTTTAATAGTAACTATTTTGGTCATTTAGAAATGAAAATAAAGATATCAAAATGGTTACTTTTATAAAATATAATGACAAAAATGAACATTTTAAAAATACATGGATTAAAGTTAAAAATATAAAAATAAAAATAAAAATATCAAAAATATAGGAACTAAAATTAACAAGTCAAATTTTTAGTGAACTTTATATATATATATATATATATATATATATAGTAATAGATTATAATAAGTAAAGAAATTGTAAAATGTGTAAAGTGGGTAATCCAAATATAAGGTCATCACTGCCTGTATTATCTAAACACATCCCCATGTCGTTTTCGATGTGTGTAAGCCAAATATATGTTTATATCATTCACTCTTATTTTTCAATATGTCTCTCTTCAAAATCTTTCTTAGTGTCAGATCACGTGAGGTACTTACCTTTGTAGGGTATATCTGAAAATTATTCGAGGAGGCTGTCAGTATTTTTGTATCGTTTGATGTAACAATACGAAGAAAAAGAAAATTGTAAAAGTATCTCGAATTTATATATTTAAATATATATATATATATAGATTTTAGAATTAATCTATGATCCATGATTATTATAAAGTTAGCTAGCAAATAAGGTTGGTTAATCATCTCGATTTTTATTTTTTATTTTTAGAATGTGTTTACAAATATAAACTAATAGTAAGAAAATTGAGAAGAAACAAACATAAATTTCTTTTGAAAGATAATTAAAAATAAAATTGTTATCAAATGAGACTTAAATATTAGTTTATTACTCGGAATCGGAATCTATTTAAAATCGTAATCTACTAATTCAAACCTTCTAATATAGAAGACGGTCTTTTGAATCCAAACGTCTTAAACCTGCATTTTAAATTCGATGTTTTTAGATAAAGTTGATTTAATATATAATAATTATTATTAATTTTTTTTTGTTTTTTTGGGGTTAAAGGTGCCACCTAGACCAAACACGTACATGCCATTTGGAAATGGAGTGCACTCGTGTCCAGGCAGTGAGATGGCTAAGCTTGAAATGCTTGTTCTTCTCCACCACCTCACCACCACTTACAGGTTTTTTTTTTCTCTTTTTCCCACTACTTTTTCTATAGATTTGAAAATATTTTTATATCTAATAGAGCACGACTATCAATTTCTTGTTTAAAATGCAAATAAGATTTCATATTAATTAGAAAAGAAAGTAATTATAAATATATAAATAAGGACAAATAATTTTAGAAATAAAATCATCAAAATTTATGTCCAAAAGTGAATAGTATCATACTATGGTGGAGATAAGGTGAAAGGTTAGTTGTTATTATTATCACTTCTTTCAATAAATTTTGATTTAATTGATATCTCTTTCTTTCTAAGAAAATCCTATTTTTATCTATACGAATATTATTCTCTCTTTTAAGTAATTTGATTTTTAGTGTTGATCTACACTAAAAATCAAATGAATGAAGTTCGTATATCCACTACTATTACAAAGGTGGTTCATGAGCTATACTTAAATTGGTCTCTTTTCGAGGTCTAAAACCCTAATTTTGGATTATTTTATTAGTCTTTTTTCTTCAAGTTTAATTTATACTTTAGACTGGAGGGAGAAATGGTTTTAATTATTAGTATGATGTATCTATCAACCTCTTGAAACTTGATCTTACTTTTTGCTTGTTAACTAGTATTTTGTCTTTCTATCTTCGATAAAAGAAAAAAATTAATTTTATTTTTAAAATTTGGTTAAGAATCCAATTATTTAAGATATATAAAAATTATGAGAAAACAAACATAATTTTCAAGAACTAAACCGTTATTAAACATGATTAATTTTTTTTTTTTGTTGACTAGAAGGTCAAATCTTTTCATTGGTTGTGGTCCTCTCTCCTTGAATGTTCCTTAAGCTGTGGAACTGTGGTCCTTTTTTTCCCTCTATATCTCTCTTATCTAAGGGCAGCTTACCCTTAAAATATAAGGCTCCCCTTTCCTTCCTTTCTCTTATCTGAATCTATATAAAGGGCAGTACCTCTTACCCTTGGAATAAAAGGCTTCTTTGTCCCTCCCTCCCACACACACCCCCCACCATACCCTCAAAATAAAATAGAAAAATATTCAGATGTTTTCTAGGCTGCATCTATTTCTGTGCCATCTAAAAAATTATTCCGTAAAAATTATCACATGACAATTTTTATTTTTAGAGCAGTCTAGGTTTCACCTAATCATTTGTCCCTTAAAATATTATCAAAAGGGTCTTTTCTTTTTGCAAAGGTCTTTAATTATCCTTAATATTAAAGGACCCCTTTACCATCTCATGTGGGTTTACTATGTTTTATTTTTCTTCCTTGAACACAAATTATGGAAAAAAAATGTCTTGGATATAGGACATGTTTTGAATATTTTTTTTAAACAAATCAATAATTCTTTATATAGAAAGATTTTTTTTTTATTGAGTCTAGTAAAAAAAAATGAAGTTATTGATTATAAATTTGATAGTTTGAAAAAGAAAGTTAGAATTTAGTTTTATGGTTTGATAAAATCTCATAAATAGTTTATATAGTTTGATATAATACCCTAAAGACTATTTATGAAGATTATATCAAATTATAGGGACTAAATTTTAATCTTCTTTCTGACTAAATTTATATTTTAACCAAAATTATTTATTTTCTTAAATTTTAAATTTCTTGGAGGAAACAAGGTAGGATTGTTTTTTTGTTGCATGCATTAGTACACGTGGCATACATGCATCAAATCTTAGTATAGGGGGGAAAATACTACACATAATATTACATTCCCAATAACCTCTTCGTTGCTTGTCACCTCCTACTCATATTGCTTTTCATGAGTAATAATGAATTATAGTTTTTTATATATCTTTTAAAATTTTTGTGGTTAAACATGACTCAATTAGAGAGAATTATAAGATAATTAATCTATTCTTCATCATATATATCTTTCTAGTATCTTTTTTAATTTTCAATTAGGATTAAAATATTTTTTGATATATTTCCTAATATGATTAAAAAAAAAAAAGTCACTTTATACTTTTTTGAAAAATAGTTTTATTTTGTTGATGGGTTATATTTTAAGCTTATCTAGAAATAATTTGTTCAATTGAAGTGACATGACTCATATATGCCTGACTAGCCATTATGATCAGCTTTTCAGTCTTGTTATTTGTATTTTTTAAGGTCATATCATTTAATTTAATAAATATATGTCTTTTTATGTGTGACTGAAAATCATTCCATATGTGTAAGTCTCACATCACTTCTTCAATTTATTAAGTCATATTTCTTTTAGGAAAAAAAAAACTTATAAATAAGCAATGTTTTTTTTATTTAAATTATTACTTGTTTGAGATATTTTTTAGAATGTAATAATTTTAGGAGTGTGTTGGATTGAAAAAATAATATGATAAGACTTGAATTCTAACCCCCAAGAAAACAAGAATAAATCTTTTTTCTTGAGAAATTTTTAAAAATAGAAAAATAAGAGAAATTATTTACACAAAATAGCACAAGTTTTAGATAGTTATAATAGACTCTAATAGAAGTCTATCAAGGTCTATCAGTTATAGAAATAATAGAAGTTTATCAGGATAGATGTTGATAGAAGTCTATCAATGTCTATCAGTATCTTTTTTGCTATTTTCTGTAAATAGTTTGACATTTTTTCTATCTATGAAAATTTCTCTTTTTTTTTTTACTATGTAATATAGATAAATTGTGAGATTACTTAATAAAACCCAAAATTAAAGAAAAATAAAATCCGATAATTTAAGATAATTAACTTAAATTTAGAAAAATTGTTATAAACAGAAAAAATATCAAATTATTTACAAATATAGAAAATTTTTACTATTTATCCGTGATAGATCACGATGGAATTTTATCGCTGGAACGATAGACATAGTAGACAGAAAAAAATTTCTATATTTATAAATAGTTTGACTCATTTTTTTATATTTGAAAGCATCCCTTAAATTTACTTAAAACTCGAGATATTACACTAGTTAAGACATCCAACTAAAAAATAGTGAAACTCAAGAGAACAAATTTAAATTTCAAGATTCACTCAAAATTGTGGATTCTAATTGTCTACATGTAAGCTGACTCGAATAGTGAGTTACATCCTTGAGATTCCATTTCCCAAAATCCCAATCATTTGGTCTCAAAATATCCTAACCCTAATCCATTTCTTCATCTCTCTCTCTCATACTTTTCTCTGTCAAATAACATACAAATGGCGGAGAATTGGCCTGTCAAGGCTTCGGCCCATGGCGACAAAACAAAGCCGCCATAGCCAGCTGTAAGGAACAATCATAATTCTCTCTATCACCACTCACCAACCCATTTCTTCCATCTCCACGTTTGAACGTTTAACACCTCATGTTTTTCTTTTGCTGATCCAGCCGTCAAGAGTGGTAGGAATATTGATTCTTTCATCTGGGTTTTACGTTAAATGTTGATTATGATGTTTCTTTATACTCATGACTGTTTGTGGAAATGTCGCAATTGAATTATATGGGGGGTTTTTAGGTAATGCCTGTAAAATGTTTGTGAAAATGTCACTGTTGAATTTATAATGTAGGCTTTGAAATGTCTGACTGAATTATAATGGTGGATTAATTAGTTGTTTGCGTAATCTGCATACGAAGTGTTTGTGAAAATGTCTCAGTTGAATTGTGATGTTGCTTGCCAGGTGTTTGTGAAAATGTCTCAATGAGTTTATAGAGAGATAATTAGTTATGTTGGCTAATCTTGGCTTTATTTTTGTTTGGGGAATCAGATGGAAAGTAGTGGGAGAGGAATCTGGAATACAATACGGTCCATTTCCTGTACCTAAAGGTGGTTTACCCATAAAAGTATTTTCAAGGAAGAGGAAGATAATTACATCTTAG

mRNA sequence

ATTTTTGCATACAAATTTTGCTCTCCATAAATTAAATCTTTCTTTCTTTCTCCATTTCCACACAAAATAATAATAATAATGCTTCATCTTCTCTGTTTATTTGGTCTCTTTGTCTTTCTCTTATGTCTTCAATGGCGCCAACCCAAACACAAACTTCTTCCTCCTGGCTCTATGGGTTGGCCTTACATCGGCGAAACTTTTAAACTCTACACCCAAAATCCAAACTCGTTCTTCTCCATTAGGCAAAAAAGATATGGAGATGTTTTTAAGACACATATATTGGGTTGTCCTTGTGTAATGATTTCAAGTCCAAAGGCTGCAAGAGTTGTGCTTGTGAGTAAAGCTCATCTATTCAAGCCAACTTATCCACCAAGCAAAGAGAAAATGATAGGTCCTCAAGCTTTGTTTTTCCATCAAGGTCCTTACCATTCTCACCTTAAGAAGCTCATTCAAGCTTCCTTTTTGCCATCTGCTATAAAAGCCTCTATCTCTCAAATTGAGAACATTGTTCTCCACCTTCTCCCCTCTTGGAATAACTCCCAAATCAACACCTTGCAACAAATGAAAAGGTTTGCTTTTGATGTGGCAATGATATCAGCTTTTGGAGACCAACAAGATTTGGAAATTGAAAGAATCAAACATTTGTACCAATGCCTTGAAAAAGGCTACAATTCTATGCCTCTTGATCTTCCTGGAACTCCTTTCCGCAAAGCAATGAAGGCGAGGAAGGTGCTAAGTGAGACATTGGGGAAATTGATAGAGAAGAGACGGCGGAGCAAAGAGCATGGAGGTGGGTTGCTGGCGGTGCTACTGAGCGGTGGAGGTGGCGACGGTGGAGAGGATGAGAAAAAGAAGCTGAGTGATTCACAAATAGCAGATAATATAATAGGAGTAATATTTGCAGCACAAGACACGACAGCCAGTGTTTTAACATGGATTCTCAAGTATTTACACGACAATCACCATCTTCTTCAAGCTGTTCAGGTGCCACCTAGACCAAACACGTACATGCCATTTGGAAATGGAGTGCACTCGTGTCCAGGCAGTGAGATGGCTAAGCTTGAAATGCTTGTTCTTCTCCACCACCTCACCACCACTTACAGATGGAAAGTAGTGGGAGAGGAATCTGGAATACAATACGGTCCATTTCCTGTACCTAAAGGTGGTTTACCCATAAAAGTATTTTCAAGGAAGAGGAAGATAATTACATCTTAG

Coding sequence (CDS)

ATGCTTCATCTTCTCTGTTTATTTGGTCTCTTTGTCTTTCTCTTATGTCTTCAATGGCGCCAACCCAAACACAAACTTCTTCCTCCTGGCTCTATGGGTTGGCCTTACATCGGCGAAACTTTTAAACTCTACACCCAAAATCCAAACTCGTTCTTCTCCATTAGGCAAAAAAGATATGGAGATGTTTTTAAGACACATATATTGGGTTGTCCTTGTGTAATGATTTCAAGTCCAAAGGCTGCAAGAGTTGTGCTTGTGAGTAAAGCTCATCTATTCAAGCCAACTTATCCACCAAGCAAAGAGAAAATGATAGGTCCTCAAGCTTTGTTTTTCCATCAAGGTCCTTACCATTCTCACCTTAAGAAGCTCATTCAAGCTTCCTTTTTGCCATCTGCTATAAAAGCCTCTATCTCTCAAATTGAGAACATTGTTCTCCACCTTCTCCCCTCTTGGAATAACTCCCAAATCAACACCTTGCAACAAATGAAAAGGTTTGCTTTTGATGTGGCAATGATATCAGCTTTTGGAGACCAACAAGATTTGGAAATTGAAAGAATCAAACATTTGTACCAATGCCTTGAAAAAGGCTACAATTCTATGCCTCTTGATCTTCCTGGAACTCCTTTCCGCAAAGCAATGAAGGCGAGGAAGGTGCTAAGTGAGACATTGGGGAAATTGATAGAGAAGAGACGGCGGAGCAAAGAGCATGGAGGTGGGTTGCTGGCGGTGCTACTGAGCGGTGGAGGTGGCGACGGTGGAGAGGATGAGAAAAAGAAGCTGAGTGATTCACAAATAGCAGATAATATAATAGGAGTAATATTTGCAGCACAAGACACGACAGCCAGTGTTTTAACATGGATTCTCAAGTATTTACACGACAATCACCATCTTCTTCAAGCTGTTCAGGTGCCACCTAGACCAAACACGTACATGCCATTTGGAAATGGAGTGCACTCGTGTCCAGGCAGTGAGATGGCTAAGCTTGAAATGCTTGTTCTTCTCCACCACCTCACCACCACTTACAGATGGAAAGTAGTGGGAGAGGAATCTGGAATACAATACGGTCCATTTCCTGTACCTAAAGGTGGTTTACCCATAAAAGTATTTTCAAGGAAGAGGAAGATAATTACATCTTAG

Protein sequence

MLHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLKKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGLLAVLLSGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQAVQVPPRPNTYMPFGNGVHSCPGSEMAKLEMLVLLHHLTTTYRWKVVGEESGIQYGPFPVPKGGLPIKVFSRKRKIITS

Homology

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BLAST of Lsi02G013430 vs. ExPASy Swiss-Prot
Match: O81077 (Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 3.5e-131
Identity = 252/462 (54.55%), Postives = 309/462 (66.88%), Query Frame = 0


Query: 2   LHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGD 61
           L ++ +  + +F   L W++ + + LPPGSMG PYIGET +LYT+NPNSFF+ RQ +YGD
Sbjct: 25  LTIVVVVVVLLFKWWLHWKEQRLR-LPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGD 84

Query: 62  VFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLK 121
           +FKTHILGCPCVMISSP+AAR+VLVSKAHLFKPTYPPSKE+MIGP+ALFFHQGPYHS LK
Sbjct: 85  IFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLK 144

Query: 122 KLIQASFLPSAIKASISQIENIVLHLLPSWNNSQ-INTLQQMKRFAFDVAMISAFGDQQD 181
           +L+Q+SF+PSA++ ++S IE +VL  L SW + + INTL+ MKR+AFDVA++SAFGD+++
Sbjct: 145 RLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEE 204

Query: 182 -LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGG 241
              I+ IK LYQ LE+GYNSMPLDLPGT F K+MKAR  LSE L K+IEKRR +    GG
Sbjct: 205 PTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGG 264

Query: 242 LLAVLLSGGGGDGGEDEKKK-LSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLL 301
           LL VLL      G +D+K+  LSDSQIADNIIGVIFAA DTTASVLTW+LKYLHD+ +LL
Sbjct: 265 LLGVLL------GAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLL 324

Query: 302 QAV--------------------------------------------------------- 361
           Q V                                                         
Sbjct: 325 QEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYD 384

Query: 362 -----------------------------------QVPPRPNTYMPFGNGVHSCPGSEMA 369
                                              +V P+P TYMPFGNGVHSCPGSE+A
Sbjct: 385 GYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELA 444

[Close]

BLAST of Lsi02G013430 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 4.4e-110
Identity = 210/461 (45.55%), Postives = 278/461 (60.30%), Query Frame = 0


Query: 7   LFGLFVFLLCLQWRQPKHKL-LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKT 66
           LF  F+  L  Q R    KL LPPG+MGWPY+GETF+LY+Q+PN FF  +QKRYG VFKT
Sbjct: 15  LFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKT 74

Query: 67  HILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLKKLIQ 126
           H+LGCPCVMISSP+AA+ VLV+K+HLFKPT+P SKE+M+G QA+FFHQG YH+ L+KL+ 
Sbjct: 75  HVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVL 134

Query: 127 ASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQDLEIER 186
            +F+P +I+  +  IE+I    L SW  + INT Q+MK + F+VA++S FG  + L  E 
Sbjct: 135 RAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKDEVLYRED 194

Query: 187 IKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGLLAVLL 246
           +K  Y  LEKGYNSMP++LPGT F K+MKARK LS+ L +++ +RR++      LL   +
Sbjct: 195 LKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM 254

Query: 247 SGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQAV---- 306
                     +K++L+D QIADNIIGVIFAA+DTTASV++WILKYL +N ++L+AV    
Sbjct: 255 G---------DKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQ 314

Query: 307 ------------------------------------------------------------ 366
                                                                       
Sbjct: 315 MAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGW 374

Query: 367 ---------------------------QVPPRPNTYMPFGNGVHSCPGSEMAKLEMLVLL 376
                                      +V P+PNT+MPFGNG HSCPG+E+AKLEM +++
Sbjct: 375 KVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMI 434

[Close]

BLAST of Lsi02G013430 vs. ExPASy Swiss-Prot
Match: Q9FH76 (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 6.4e-109
Identity = 202/431 (46.87%), Postives = 263/431 (61.02%), Query Frame = 0


Query: 27  LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKTHILGCPCVMISSPKAARVVLV 86
           LPPG+MG+PY+GETF+LY+Q+PN FF+ +Q+RYG VFKTH+LGCPCVMISSP+AA+ VLV
Sbjct: 36  LPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLV 95

Query: 87  SKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLKKLIQASFLPSAIKASISQIENIVLH 146
           +K+HLFKPT+P SKE+M+G QA+FFHQG YHS L+KL+  +F+P AI+  +  IE+I   
Sbjct: 96  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQE 155

Query: 147 LLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQDLEIERIKHLYQCLEKGYNSMPLDLPG 206
            L SW+ +Q+NT Q+MK + F+VA+IS  G  +    E +K  Y  LEKGYNSMP++LPG
Sbjct: 156 SLNSWDGTQLNTYQEMKTYTFNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINLPG 215

Query: 207 TPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGLLAVLLSGGGGDGGEDEKKKLSDSQIA 266
           T F KAMKARK L++ L  ++ KRR++      LL   +         ++K  L+D QIA
Sbjct: 216 TLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGSFM---------EDKAGLTDEQIA 275

Query: 267 DNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQAV------------------------- 326
           DNIIGVIFAA+DTTASVLTWILKYL DN  +L+AV                         
Sbjct: 276 DNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKM 335

Query: 327 ------------------------------------------------------------ 367
                                                                       
Sbjct: 336 PLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGK 395

[Close]

BLAST of Lsi02G013430 vs. ExPASy Swiss-Prot
Match: K4CI52 (Abscisic acid 8'-hydroxylase CYP707A2 OS=Solanum lycopersicum OX=4081 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.5e-107
Identity = 211/461 (45.77%), Postives = 275/461 (59.65%), Query Frame = 0


Query: 2   LHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGD 61
           L LL +  +F FL     + P    LPPG++G PYIGETF+LY+QNPN FF+ + K+YG 
Sbjct: 15  LTLLLIHSIFKFLAFASKKLP----LPPGTLGLPYIGETFQLYSQNPNVFFASKVKKYGS 74

Query: 62  VFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLK 121
           +FKT+ILGCPCVMISSP+AA+ VLV+KA+LFKPT+P SKE+M+G QA+FFHQG YH+ L+
Sbjct: 75  IFKTYILGCPCVMISSPEAAKQVLVTKANLFKPTFPASKERMLGKQAIFFHQGDYHAKLR 134

Query: 122 KLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQDL 181
           KL+  +F P +I+  I  IE+I +  L S+    INT Q+MK + F+VA+IS FG  + L
Sbjct: 135 KLVLQAFKPDSIRNIIPDIESIAITSLESFQGRLINTYQEMKTYTFNVALISIFGKDEFL 194

Query: 182 EIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGLL 241
             E +K  Y  LEKGYNSMP++LPGT F KAMKARK L++ + K+I  RR  K   G LL
Sbjct: 195 YREELKKCYYILEKGYNSMPINLPGTLFNKAMKARKELAKIVAKIISTRREMKIDHGDLL 254

Query: 242 AVLLSGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQAV 301
              +          +K+ L+D QIADN+IGVIFAA+DTTASVLTWILKYL +N  +LQAV
Sbjct: 255 GSFMG---------DKEGLTDEQIADNVIGVIFAARDTTASVLTWILKYLGENPSVLQAV 314

Query: 302 ------------------------------------------------------------ 361
                                                                       
Sbjct: 315 TEEQENIMRKKEVNGEEKVLNWQDTRQMPMTTRVIQETLRVASILSFTFREAVEDVEFEG 374

Query: 362 ----------------------------------QVPPRPNTYMPFGNGVHSCPGSEMAK 369
                                             +V P+PNT+MPFGNGVHSCPG+++AK
Sbjct: 375 YLIPKGWKVLPLFRNIHHSPDNFPEPEKFDPSRFEVSPKPNTFMPFGNGVHSCPGNDLAK 434

[Close]

BLAST of Lsi02G013430 vs. ExPASy Swiss-Prot
Match: Q09J79 (Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A5 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.5e-105
Identity = 199/439 (45.33%), Postives = 265/439 (60.36%), Query Frame = 0


Query: 27  LPPGSMGWPYIGETFKLY-TQNPNSFFSIRQKRYGDVFKTHILGCPCVMISSPKAARVVL 86
           LPPGSMGWPY+GETF+LY ++NPN FF+ ++ +YG +FKTHILGCPCVM+SSP+AAR VL
Sbjct: 39  LPPGSMGWPYVGETFQLYSSKNPNVFFNKKRNKYGPIFKTHILGCPCVMVSSPEAARFVL 98

Query: 87  VSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLKKLIQASFLPSAIKASISQIENIVL 146
           V++AHLFKPT+P SKE+M+GPQA+FF QG YH+HL++++  +F P +I+AS+  IE I L
Sbjct: 99  VTQAHLFKPTFPASKERMLGPQAIFFQQGDYHAHLRRIVSRAFSPESIRASVPAIEAIAL 158

Query: 147 HLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQDLEIERIKHLYQCLEKGYNSMPLDLP 206
             L SW+   +NT Q+MK +A +VA++S FG+++   IE +K  Y  LEKGYNSMP++LP
Sbjct: 159 RSLHSWDGQFVNTFQEMKTYALNVALLSIFGEEEMRYIEELKQCYLTLEKGYNSMPVNLP 218

Query: 207 GTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGLLAVLLSGGGGDGGEDEKKKLSDSQI 266
           GT F KAMKARK L   +  +I   RR ++ G  LL   + G         ++ L+D+QI
Sbjct: 219 GTLFHKAMKARKRLGAIVAHII-SARRERQRGNDLLGSFVDG---------REALTDAQI 278

Query: 267 ADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQAV------------------------ 326
           ADN+IGVIFAA+DTTASVLTW++K+L D+  +L+AV                        
Sbjct: 279 ADNVIGVIFAARDTTASVLTWMVKFLGDHPAVLKAVTEEQLQIAKEKEASGEPLSWADTR 338

Query: 327 ------------------------------------------------------------ 373
                                                                       
Sbjct: 339 RMKMTSRVIQETMRVASILSFTFREAVEDVEYQGYLIPKGWKVLPLFRNIHHNPDHFPCP 398

[Close]

BLAST of Lsi02G013430 vs. ExPASy TrEMBL
Match: A0A1S3C5P5 (abscisic acid 8'-hydroxylase 2 OS=Cucumis melo OX=3656 GN=LOC103497144 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 6.9e-191
Identity = 350/467 (74.95%), Postives = 361/467 (77.30%), Query Frame = 0


Query: 1   MLHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60
           ML LL LF LF+ LLCLQW  PKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG
Sbjct: 1   MLLLLGLFSLFILLLCLQWSHPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60

Query: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHL 120
           DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHS+L
Sbjct: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSYL 120

Query: 121 KKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180
           KKLIQ+SFLPSAIK SISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD
Sbjct: 121 KKLIQSSFLPSAIKHSISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180

Query: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGL 240
           LEIERIKHLYQCLEKGYNSMPLDLPGTPF KAMKARKVLSETLGK+IEKRRR+KEHGGGL
Sbjct: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFHKAMKARKVLSETLGKMIEKRRRNKEHGGGL 240

Query: 241 LAVLLSGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQA 300
           LAVLLSGGGG+  E+EKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLL+A
Sbjct: 241 LAVLLSGGGGE--EEEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLEA 300

Query: 301 V----------------------------------------------------------- 360
           V                                                           
Sbjct: 301 VKKEQDAIYERKLCEGKRGLSWDDTRRMPFTSRVILETLRRASVVSFTFREAVEEVEFEG 360

Query: 361 ----------------------------------QVPPRPNTYMPFGNGVHSCPGSEMAK 375
                                             + PPRPNTYMPFGNGVHSCPGSEMAK
Sbjct: 361 YLIPKGWKVLPLFRTIHHSPDFFPHPHNFDPSRFEEPPRPNTYMPFGNGVHSCPGSEMAK 420

[Close]

BLAST of Lsi02G013430 vs. ExPASy TrEMBL
Match: A0A6J1G973 (abscisic acid 8'-hydroxylase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111452117 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 5.3e-183
Identity = 340/469 (72.49%), Postives = 355/469 (75.69%), Query Frame = 0


Query: 1   MLHLLCLFG-LFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY 60
           ML LL LFG L + LLCLQWR P H LLPPGSMGWPY GETFKLYTQNPNSFFSIRQKRY
Sbjct: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYTGETFKLYTQNPNSFFSIRQKRY 60

Query: 61  GDVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSH 120
           GDVFKTHILGCPCVMISSP+AARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHS 
Sbjct: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120

Query: 121 LKKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQ 180
           LKKLIQASFLPSAIK S+SQIENIVL LLPSW+NSQINTLQQ+KRFAFDVAMISAFGDQQ
Sbjct: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQLKRFAFDVAMISAFGDQQ 180

Query: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGG 240
           DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARK+LSETLGKLIEKR+RS E+GGG
Sbjct: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240

Query: 241 LLAVLLSGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQ 300
           LLAVLL GG GDG + EK KLS+SQIADNIIGVIFAAQDTTASVLTWILKYLHD+HHLL+
Sbjct: 241 LLAVLL-GGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLE 300

Query: 301 AV---------------------------------------------------------- 360
           AV                                                          
Sbjct: 301 AVKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEEVEFE 360

Query: 361 -----------------------------------QVPPRPNTYMPFGNGVHSCPGSEMA 376
                                              +VPPRPNTYMPFGNGVHSCPGSEMA
Sbjct: 361 GYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMA 420

[Close]

BLAST of Lsi02G013430 vs. ExPASy TrEMBL
Match: A0A6J1KC93 (abscisic acid 8'-hydroxylase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492574 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.0e-182
Identity = 341/473 (72.09%), Postives = 354/473 (74.84%), Query Frame = 0


Query: 1   MLHLLCLFG----LFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60
           ML LL LFG    L + LLC QWR P H LLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ
Sbjct: 1   MLPLLLLFGLLLLLLLLLLCHQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60

Query: 61  KRYGDVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120
           KRYGDVFKTHILGCPCVMISSP+AARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY
Sbjct: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120

Query: 121 HSHLKKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFG 180
           HS LKKLIQASFLPSAIK S+SQIENIVL LLPSW+NSQINTLQQMKRFAFDVAMISAFG
Sbjct: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180

Query: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEH 240
           DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARK+LSETLGKLIEKR+RS E+
Sbjct: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240

Query: 241 GGGLLAVLLSG-GGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNH 300
           GGGLLAVLL G G GDG + EK KL+DSQIADNIIGVIFAAQDTTASVLTWILKYLHD+H
Sbjct: 241 GGGLLAVLLGGDGDGDGEKVEKNKLTDSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300

Query: 301 HLLQAV------------------------------------------------------ 360
           HLL+AV                                                      
Sbjct: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEE 360

Query: 361 ---------------------------------------QVPPRPNTYMPFGNGVHSCPG 376
                                                  +VPPRPNTYMPFGNGVHSCPG
Sbjct: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFTHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPG 420

[Close]

BLAST of Lsi02G013430 vs. ExPASy TrEMBL
Match: A0A6J1K5S5 (abscisic acid 8'-hydroxylase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492574 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.9e-181
Identity = 340/473 (71.88%), Postives = 353/473 (74.63%), Query Frame = 0


Query: 1   MLHLLCLFG----LFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60
           ML LL LFG    L + LLC QWR P H LLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ
Sbjct: 1   MLPLLLLFGLLLLLLLLLLCHQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQ 60

Query: 61  KRYGDVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120
           KRYGDVFKTHILGCPCVMISSP+AARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY
Sbjct: 61  KRYGDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPY 120

Query: 121 HSHLKKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFG 180
           HS LKKLIQASFLPSAIK S+SQIENIVL LLPSW+NSQINTLQQMKRFAFDVAMISAFG
Sbjct: 121 HSRLKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFG 180

Query: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEH 240
           DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARK+LSETLGKLIEKR+RS E+
Sbjct: 181 DQQDLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEY 240

Query: 241 GGGLLAVLLSG-GGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNH 300
           GGGLLAVLL G G GDG + EK KL+DSQIADNIIGVIFAAQDTTASVLTWILKYLHD+H
Sbjct: 241 GGGLLAVLLGGDGDGDGEKVEKNKLTDSQIADNIIGVIFAAQDTTASVLTWILKYLHDDH 300

Query: 301 HLLQAV------------------------------------------------------ 360
           HLL+AV                                                      
Sbjct: 301 HLLEAVKKEQDAIFEERVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEE 360

Query: 361 ---------------------------------------QVPPRPNTYMPFGNGVHSCPG 376
                                                  +V PRPNTYMPFGNGVHSCPG
Sbjct: 361 VEFEGYLIPRGWKVLPLFRTIHHSAHFFTHPEKFDPSRFEVLPRPNTYMPFGNGVHSCPG 420

[Close]

BLAST of Lsi02G013430 vs. ExPASy TrEMBL
Match: A0A6J1DLH4 (abscisic acid 8'-hydroxylase 2 OS=Momordica charantia OX=3673 GN=LOC111022241 PE=3 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 6.7e-178
Identity = 327/465 (70.32%), Postives = 345/465 (74.19%), Query Frame = 0


Query: 5   LCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFK 64
           L LF  F  LL LQWR P+HKLLPPGS+GWPYIGETFKLYTQNPNSFFS+RQKRYGDVFK
Sbjct: 4   LLLFSSFFLLLLLQWRHPRHKLLPPGSVGWPYIGETFKLYTQNPNSFFSVRQKRYGDVFK 63

Query: 65  THILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLKKLI 124
           THILGCPCVMISSP+AARVVLVSKAHLFKPTYPPSKEKMIGP+ALFFHQG YHS LKKL+
Sbjct: 64  THILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPEALFFHQGSYHSCLKKLV 123

Query: 125 QASFLPSAIKASISQIENIVLHLLPSWNNS-QINTLQQMKRFAFDVAMISAFGDQQDLEI 184
           QASFLPSAI+ S+SQIE IVL LLPSWNNS QINTL QMKRFAFDVAMISAFG+QQ+LEI
Sbjct: 124 QASFLPSAIRPSVSQIEAIVLRLLPSWNNSQQINTLHQMKRFAFDVAMISAFGEQQELEI 183

Query: 185 ERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGLLAV 244
           +RIKHLYQCLEKGYNSMP+DLPGTPFRKAMKARK+LSETLG+LIEKRRR KEHGGGLL V
Sbjct: 184 DRIKHLYQCLEKGYNSMPIDLPGTPFRKAMKARKLLSETLGRLIEKRRRRKEHGGGLLGV 243

Query: 245 LLSGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQAV-- 304
           LL GG   G E EK KLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLL AV  
Sbjct: 244 LLGGGSDGGDEAEKNKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLDAVKK 303

Query: 305 ------------------------------------------------------------ 364
                                                                       
Sbjct: 304 EQEAIFQRKLCQGNRNLTWDDTRRMPFTTRVIQETLRTASVVSFTFREAVEDVEFEGYLI 363

Query: 365 -------------------------------QVPPRPNTYMPFGNGVHSCPGSEMAKLEM 376
                                          +VPPRPNTY+PFGNGVHSCPGSE+AKLEM
Sbjct: 364 PRGWKVLPLFRTIHHSADFFPHPEKFDPSRFEVPPRPNTYLPFGNGVHSCPGSELAKLEM 423

[Close]

BLAST of Lsi02G013430 vs. NCBI nr
Match: XP_038894709.1 (abscisic acid 8'-hydroxylase 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 691.0 bits (1782), Expect = 5.6e-195
Identity = 359/470 (76.38%), Postives = 364/470 (77.45%), Query Frame = 0


Query: 1   MLHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60
           ML LLC FGL VFLLCLQWR PKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG
Sbjct: 1   MLLLLCFFGLCVFLLCLQWRHPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60

Query: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHL 120
           DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKE+MIGPQALFFHQGPYHSHL
Sbjct: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKERMIGPQALFFHQGPYHSHL 120

Query: 121 KKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180
           KKLIQASFLPSAIK SISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD
Sbjct: 121 KKLIQASFLPSAIKGSISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180

Query: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGL 240
           LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETL KLIEKRRR KE+GGGL
Sbjct: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLVKLIEKRRRRKEYGGGL 240

Query: 241 LAVLLSGGGGDGG---EDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHL 300
           LAVLL GGGG GG   E+EKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHL
Sbjct: 241 LAVLLGGGGGGGGGVEEEEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHL 300

Query: 301 LQAV-------------------------------------------------------- 360
           L+AV                                                        
Sbjct: 301 LEAVNEQEAILQSKVCEGNRGLSWDDTRCMPFTSRVILETLRRASVVSFTFREAVEEVEF 360

Query: 361 ------------------------------------QVPPRPNTYMPFGNGVHSCPGSEM 376
                                               +VPPRPNTYMPFGNGVHSCPGSEM
Sbjct: 361 EGYLIPRGWKVLPLFRTIHHSPDFFPHPDNFDPSRFEVPPRPNTYMPFGNGVHSCPGSEM 420

[Close]

BLAST of Lsi02G013430 vs. NCBI nr
Match: XP_038894708.1 (abscisic acid 8'-hydroxylase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 690.6 bits (1781), Expect = 7.3e-195
Identity = 359/471 (76.22%), Postives = 364/471 (77.28%), Query Frame = 0


Query: 1   MLHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60
           ML LLC FGL VFLLCLQWR PKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG
Sbjct: 1   MLLLLCFFGLCVFLLCLQWRHPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60

Query: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHL 120
           DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKE+MIGPQALFFHQGPYHSHL
Sbjct: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKERMIGPQALFFHQGPYHSHL 120

Query: 121 KKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180
           KKLIQASFLPSAIK SISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD
Sbjct: 121 KKLIQASFLPSAIKGSISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180

Query: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGL 240
           LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETL KLIEKRRR KE+GGGL
Sbjct: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLVKLIEKRRRRKEYGGGL 240

Query: 241 LAVLLSGGGGDGG---EDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHL 300
           LAVLL GGGG GG   E+EKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHL
Sbjct: 241 LAVLLGGGGGGGGGVEEEEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHL 300

Query: 301 LQAV-------------------------------------------------------- 360
           L+AV                                                        
Sbjct: 301 LEAVNKEQEAILQSKVCEGNRGLSWDDTRCMPFTSRVILETLRRASVVSFTFREAVEEVE 360

Query: 361 -------------------------------------QVPPRPNTYMPFGNGVHSCPGSE 376
                                                +VPPRPNTYMPFGNGVHSCPGSE
Sbjct: 361 FEGYLIPRGWKVLPLFRTIHHSPDFFPHPDNFDPSRFEVPPRPNTYMPFGNGVHSCPGSE 420

[Close]

BLAST of Lsi02G013430 vs. NCBI nr
Match: XP_008457459.1 (PREDICTED: abscisic acid 8'-hydroxylase 2 [Cucumis melo])

HSP 1 Score: 676.4 bits (1744), Expect = 1.4e-190
Identity = 350/467 (74.95%), Postives = 361/467 (77.30%), Query Frame = 0


Query: 1   MLHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60
           ML LL LF LF+ LLCLQW  PKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG
Sbjct: 1   MLLLLGLFSLFILLLCLQWSHPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60

Query: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHL 120
           DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHS+L
Sbjct: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSYL 120

Query: 121 KKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180
           KKLIQ+SFLPSAIK SISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD
Sbjct: 121 KKLIQSSFLPSAIKHSISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180

Query: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGL 240
           LEIERIKHLYQCLEKGYNSMPLDLPGTPF KAMKARKVLSETLGK+IEKRRR+KEHGGGL
Sbjct: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFHKAMKARKVLSETLGKMIEKRRRNKEHGGGL 240

Query: 241 LAVLLSGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQA 300
           LAVLLSGGGG+  E+EKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLL+A
Sbjct: 241 LAVLLSGGGGE--EEEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLEA 300

Query: 301 V----------------------------------------------------------- 360
           V                                                           
Sbjct: 301 VKKEQDAIYERKLCEGKRGLSWDDTRRMPFTSRVILETLRRASVVSFTFREAVEEVEFEG 360

Query: 361 ----------------------------------QVPPRPNTYMPFGNGVHSCPGSEMAK 375
                                             + PPRPNTYMPFGNGVHSCPGSEMAK
Sbjct: 361 YLIPKGWKVLPLFRTIHHSPDFFPHPHNFDPSRFEEPPRPNTYMPFGNGVHSCPGSEMAK 420

[Close]

BLAST of Lsi02G013430 vs. NCBI nr
Match: XP_011658248.1 (abscisic acid 8'-hydroxylase 2 isoform X1 [Cucumis sativus] >KAE8653258.1 hypothetical protein Csa_019706 [Cucumis sativus])

HSP 1 Score: 674.9 bits (1740), Expect = 4.1e-190
Identity = 349/468 (74.57%), Postives = 363/468 (77.56%), Query Frame = 0


Query: 1   MLHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60
           ML LL LF LF+ LLCLQW  PKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG
Sbjct: 1   MLLLLGLFSLFILLLCLQWSHPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYG 60

Query: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHL 120
           DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKE+MIGPQALFFHQGPYHS+L
Sbjct: 61  DVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKERMIGPQALFFHQGPYHSYL 120

Query: 121 KKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQD 180
           KKLIQ+SFLPSAIK SISQIENIVL+LLPSWNNSQINTLQQMK+FAFDVAMISAFGDQQD
Sbjct: 121 KKLIQSSFLPSAIKHSISQIENIVLNLLPSWNNSQINTLQQMKKFAFDVAMISAFGDQQD 180

Query: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGL 240
           LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGK+IEKRRR+KEHGGGL
Sbjct: 181 LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKMIEKRRRNKEHGGGL 240

Query: 241 LAVLLSGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQA 300
           LAVLLSGGGG+  E+EKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLL+A
Sbjct: 241 LAVLLSGGGGE--EEEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLEA 300

Query: 301 V----------------------------------------------------------- 360
           V                                                           
Sbjct: 301 VKKEQDAIYERKLCEGKRGLSWDDTRRMPFTSRVILETLRRASVVSFTFREAVEEVEFEG 360

Query: 361 ----------------------------------QVPPRPNTYMPFGNGVHSCPGSEMAK 376
                                             + PPRPNTYMPFGNGVHSCPGSEMAK
Sbjct: 361 YLIPKGWKVLPLFRTIHHSPDFFPHPHNFDPSRFEEPPRPNTYMPFGNGVHSCPGSEMAK 420

[Close]

BLAST of Lsi02G013430 vs. NCBI nr
Match: XP_023523016.1 (abscisic acid 8'-hydroxylase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650.6 bits (1677), Expect = 8.4e-183
Identity = 342/469 (72.92%), Postives = 354/469 (75.48%), Query Frame = 0


Query: 1   MLHLLCLFG-LFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY 60
           ML LL LFG L + LLCLQWR P H LLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY
Sbjct: 1   MLPLLLLFGLLLLLLLCLQWRHPNHHLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRY 60

Query: 61  GDVFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSH 120
           GDVFKTHILGCPCVMISSP+AARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHS 
Sbjct: 61  GDVFKTHILGCPCVMISSPEAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSR 120

Query: 121 LKKLIQASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQ 180
           LKKLIQASFLPSAIK S+SQIENIVL LLPSW+NSQINTLQQMKRFAFDVAMISAFGDQQ
Sbjct: 121 LKKLIQASFLPSAIKPSVSQIENIVLRLLPSWDNSQINTLQQMKRFAFDVAMISAFGDQQ 180

Query: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGG 240
           DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARK+LSETLGKLIEKR+RS E+GGG
Sbjct: 181 DLEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKLLSETLGKLIEKRQRSNEYGGG 240

Query: 241 LLAVLLSGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQ 300
           LLAVLL GG GDG + EK KLS+SQIADNIIGVIFAAQDTTASVLTWILKYLHD+HHLL 
Sbjct: 241 LLAVLL-GGDGDGDKVEKNKLSNSQIADNIIGVIFAAQDTTASVLTWILKYLHDDHHLLG 300

Query: 301 AV---------------------------------------------------------- 360
           AV                                                          
Sbjct: 301 AVKKEQDAIFEEKVREGGRGLTWDDTRRMALTSRVIQETLRTASVLSFTFREAVEEVEFE 360

Query: 361 -----------------------------------QVPPRPNTYMPFGNGVHSCPGSEMA 376
                                              +VPPRPNTYMPFGNGVHSCPGSEMA
Sbjct: 361 GYLIPRGWKVLPLFRTIHHSAHFFPHPEKFDPSRFEVPPRPNTYMPFGNGVHSCPGSEMA 420

[Close]

BLAST of Lsi02G013430 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 469.5 bits (1207), Expect = 2.5e-132
Identity = 252/462 (54.55%), Postives = 309/462 (66.88%), Query Frame = 0


Query: 2   LHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGD 61
           L ++ +  + +F   L W++ + + LPPGSMG PYIGET +LYT+NPNSFF+ RQ +YGD
Sbjct: 25  LTIVVVVVVLLFKWWLHWKEQRLR-LPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGD 84

Query: 62  VFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLK 121
           +FKTHILGCPCVMISSP+AAR+VLVSKAHLFKPTYPPSKE+MIGP+ALFFHQGPYHS LK
Sbjct: 85  IFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLK 144

Query: 122 KLIQASFLPSAIKASISQIENIVLHLLPSWNNSQ-INTLQQMKRFAFDVAMISAFGDQQD 181
           +L+Q+SF+PSA++ ++S IE +VL  L SW + + INTL+ MKR+AFDVA++SAFGD+++
Sbjct: 145 RLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEE 204

Query: 182 -LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGG 241
              I+ IK LYQ LE+GYNSMPLDLPGT F K+MKAR  LSE L K+IEKRR +    GG
Sbjct: 205 PTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGG 264

Query: 242 LLAVLLSGGGGDGGEDEKKK-LSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLL 301
           LL VLL      G +D+K+  LSDSQIADNIIGVIFAA DTTASVLTW+LKYLHD+ +LL
Sbjct: 265 LLGVLL------GAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLL 324

Query: 302 QAV--------------------------------------------------------- 361
           Q V                                                         
Sbjct: 325 QEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYD 384

Query: 362 -----------------------------------QVPPRPNTYMPFGNGVHSCPGSEMA 369
                                              +V P+P TYMPFGNGVHSCPGSE+A
Sbjct: 385 GYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELA 444

[Close]

BLAST of Lsi02G013430 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 469.5 bits (1207), Expect = 2.5e-132
Identity = 252/462 (54.55%), Postives = 309/462 (66.88%), Query Frame = 0


Query: 2   LHLLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGD 61
           L ++ +  + +F   L W++ + + LPPGSMG PYIGET +LYT+NPNSFF+ RQ +YGD
Sbjct: 25  LTIVVVVVVLLFKWWLHWKEQRLR-LPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGD 84

Query: 62  VFKTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLK 121
           +FKTHILGCPCVMISSP+AAR+VLVSKAHLFKPTYPPSKE+MIGP+ALFFHQGPYHS LK
Sbjct: 85  IFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLK 144

Query: 122 KLIQASFLPSAIKASISQIENIVLHLLPSWNNSQ-INTLQQMKRFAFDVAMISAFGDQQD 181
           +L+Q+SF+PSA++ ++S IE +VL  L SW + + INTL+ MKR+AFDVA++SAFGD+++
Sbjct: 145 RLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEE 204

Query: 182 -LEIERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGG 241
              I+ IK LYQ LE+GYNSMPLDLPGT F K+MKAR  LSE L K+IEKRR +    GG
Sbjct: 205 PTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGG 264

Query: 242 LLAVLLSGGGGDGGEDEKKK-LSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLL 301
           LL VLL      G +D+K+  LSDSQIADNIIGVIFAA DTTASVLTW+LKYLHD+ +LL
Sbjct: 265 LLGVLL------GAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLL 324

Query: 302 QAV--------------------------------------------------------- 361
           Q V                                                         
Sbjct: 325 QEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYD 384

Query: 362 -----------------------------------QVPPRPNTYMPFGNGVHSCPGSEMA 369
                                              +V P+P TYMPFGNGVHSCPGSE+A
Sbjct: 385 GYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELA 444

[Close]

BLAST of Lsi02G013430 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 399.4 bits (1025), Expect = 3.1e-111
Identity = 210/461 (45.55%), Postives = 278/461 (60.30%), Query Frame = 0


Query: 7   LFGLFVFLLCLQWRQPKHKL-LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKT 66
           LF  F+  L  Q R    KL LPPG+MGWPY+GETF+LY+Q+PN FF  +QKRYG VFKT
Sbjct: 15  LFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKT 74

Query: 67  HILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLKKLIQ 126
           H+LGCPCVMISSP+AA+ VLV+K+HLFKPT+P SKE+M+G QA+FFHQG YH+ L+KL+ 
Sbjct: 75  HVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVL 134

Query: 127 ASFLPSAIKASISQIENIVLHLLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQDLEIER 186
            +F+P +I+  +  IE+I    L SW  + INT Q+MK + F+VA++S FG  + L  E 
Sbjct: 135 RAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYTFNVALLSIFGKDEVLYRED 194

Query: 187 IKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGLLAVLL 246
           +K  Y  LEKGYNSMP++LPGT F K+MKARK LS+ L +++ +RR++      LL   +
Sbjct: 195 LKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM 254

Query: 247 SGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQAV---- 306
                     +K++L+D QIADNIIGVIFAA+DTTASV++WILKYL +N ++L+AV    
Sbjct: 255 G---------DKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQ 314

Query: 307 ------------------------------------------------------------ 366
                                                                       
Sbjct: 315 MAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGW 374

Query: 367 ---------------------------QVPPRPNTYMPFGNGVHSCPGSEMAKLEMLVLL 376
                                      +V P+PNT+MPFGNG HSCPG+E+AKLEM +++
Sbjct: 375 KVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMI 434

[Close]

BLAST of Lsi02G013430 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 395.6 bits (1015), Expect = 4.5e-110
Identity = 202/431 (46.87%), Postives = 263/431 (61.02%), Query Frame = 0


Query: 27  LPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVFKTHILGCPCVMISSPKAARVVLV 86
           LPPG+MG+PY+GETF+LY+Q+PN FF+ +Q+RYG VFKTH+LGCPCVMISSP+AA+ VLV
Sbjct: 36  LPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLV 95

Query: 87  SKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLKKLIQASFLPSAIKASISQIENIVLH 146
           +K+HLFKPT+P SKE+M+G QA+FFHQG YHS L+KL+  +F+P AI+  +  IE+I   
Sbjct: 96  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQE 155

Query: 147 LLPSWNNSQINTLQQMKRFAFDVAMISAFGDQQDLEIERIKHLYQCLEKGYNSMPLDLPG 206
            L SW+ +Q+NT Q+MK + F+VA+IS  G  +    E +K  Y  LEKGYNSMP++LPG
Sbjct: 156 SLNSWDGTQLNTYQEMKTYTFNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINLPG 215

Query: 207 TPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGLLAVLLSGGGGDGGEDEKKKLSDSQIA 266
           T F KAMKARK L++ L  ++ KRR++      LL   +         ++K  L+D QIA
Sbjct: 216 TLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGSFM---------EDKAGLTDEQIA 275

Query: 267 DNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQAV------------------------- 326
           DNIIGVIFAA+DTTASVLTWILKYL DN  +L+AV                         
Sbjct: 276 DNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKM 335

Query: 327 ------------------------------------------------------------ 367
                                                                       
Sbjct: 336 PLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGK 395

[Close]

BLAST of Lsi02G013430 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 378.3 bits (970), Expect = 7.5e-105
Identity = 203/455 (44.62%), Postives = 267/455 (58.68%), Query Frame = 0


Query: 4   LLCLFGLFVFLLCLQWRQPKHKLLPPGSMGWPYIGETFKLYTQNPNSFFSIRQKRYGDVF 63
           +LCL  L V ++  + ++     LPPGSMGWPY+GET +LY+QNPN FF+ +QKRYG++F
Sbjct: 13  ILCL--LLVRVIVSKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSKQKRYGEIF 72

Query: 64  KTHILGCPCVMISSPKAARVVLVSKAHLFKPTYPPSKEKMIGPQALFFHQGPYHSHLKKL 123
           KT ILG PCVM++SP+AAR VLV+ AH+FKPTYP SKEK+IGP ALFFHQG YHSH++KL
Sbjct: 73  KTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKL 132

Query: 124 IQASFLPSAIKASISQIENIVLHLLPSWNNSQI-NTLQQMKRFAFDVAMISAFGDQQDLE 183
           +Q+SF P  I+  I  IE+I L  L SW N  I +T Q+MK+FAFDV +++ FG  +   
Sbjct: 133 VQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHLESSY 192

Query: 184 IERIKHLYQCLEKGYNSMPLDLPGTPFRKAMKARKVLSETLGKLIEKRRRSKEHGGGLLA 243
            E +KH Y  ++KGYNS P+ LPGT + KA+ ARK L   + ++I +RR  +      L 
Sbjct: 193 KEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQTDFLG 252

Query: 244 VLLSGGGGDGGEDEKKKLSDSQIADNIIGVIFAAQDTTASVLTWILKYLHDNHHLLQAV- 303
            LL+     G     + L+  QIADNIIGV+FAAQDTTAS LTWILKYLHD+  LL+AV 
Sbjct: 253 HLLNFKNEKG-----RVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVK 312

Query: 304 ------------------------------------------------------------ 363
                                                                       
Sbjct: 313 AEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLI 372

Query: 364 -------------------------------QVPPRPNTYMPFGNGVHSCPGSEMAKLEM 366
                                          +V P+PNT+MPFG+GVH+CPG+E+AKL++
Sbjct: 373 PKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAKLQI 432

The following BLAST results are available for this feature:

BLAST of Lsi02G013430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81077	3.5e-131	54.55	Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 ...	[more]
Q949P1	4.4e-110	45.55	Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 ...	[more]
Q9FH76	6.4e-109	46.87	Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 ...	[more]
K4CI52	3.5e-107	45.77	Abscisic acid 8'-hydroxylase CYP707A2 OS=Solanum lycopersicum OX=4081 GN=CYP707A...	[more]
Q09J79	2.5e-105	45.33	Abscisic acid 8'-hydroxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]

BLAST of Lsi02G013430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C5P5	6.9e-191	74.95	abscisic acid 8'-hydroxylase 2 OS=Cucumis melo OX=3656 GN=LOC103497144 PE=3 SV=1	[more]
A0A6J1G973	5.3e-183	72.49	abscisic acid 8'-hydroxylase 2-like OS=Cucurbita moschata OX=3662 GN=LOC11145211...	[more]
A0A6J1KC93	2.0e-182	72.09	abscisic acid 8'-hydroxylase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1K5S5	2.9e-181	71.88	abscisic acid 8'-hydroxylase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1DLH4	6.7e-178	70.32	abscisic acid 8'-hydroxylase 2 OS=Momordica charantia OX=3673 GN=LOC111022241 PE...	[more]

BLAST of Lsi02G013430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038894709.1	5.6e-195	76.38	abscisic acid 8'-hydroxylase 2 isoform X2 [Benincasa hispida]	[more]
XP_038894708.1	7.3e-195	76.22	abscisic acid 8'-hydroxylase 2 isoform X1 [Benincasa hispida]	[more]
XP_008457459.1	1.4e-190	74.95	PREDICTED: abscisic acid 8'-hydroxylase 2 [Cucumis melo]	[more]
XP_011658248.1	4.1e-190	74.57	abscisic acid 8'-hydroxylase 2 isoform X1 [Cucumis sativus] >KAE8653258.1 hypoth...	[more]
XP_023523016.1	8.4e-183	72.92	abscisic acid 8'-hydroxylase 2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi02G013430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29090.1	2.5e-132	54.55	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	2.5e-132	54.55	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT4G19230.1	3.1e-111	45.55	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT5G45340.1	4.5e-110	46.87	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT3G19270.1	7.5e-105	44.62	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 320..343 score: 23.66 coord: 56..75 score: 31.22 coord: 310..320 score: 44.1
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 308..348 e-value: 4.9E-9 score: 35.4 coord: 28..302 e-value: 5.6E-30 score: 104.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 19..301 e-value: 1.1E-64 score: 220.9 coord: 302..371 e-value: 3.0E-12 score: 48.0
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 22..371
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 307..369
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 6..302
None	No IPR available	PANTHER	PTHR24286:SF220	ABSCISIC ACID 8'-HYDROXYLASE 2	coord: 307..369
None	No IPR available	PANTHER	PTHR24286:SF220	ABSCISIC ACID 8'-HYDROXYLASE 2	coord: 6..302
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 313..322

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G013430.1	Lsi02G013430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen