Lsi02G013300 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G013300 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.9e-101
Identity = 202/388 (52.06%), Postives = 266/388 (68.56%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           ME E++ IL  +   E++ P          P+   + +      CAF+R EH   E+ +R
Sbjct: 1   MELEQICILKPDDEEEMESP---------SPSKTEENLGVVPLSCAFTRQEHCVSETKER 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
            +S  +L  LIF Y I + V+I+GG +ANSLAVMTDAAHLLSDVAG  VSL A+ VS WE
Sbjct: 61  EESTRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDVAGLCVSLLAIKVSSWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
           A P+ SFG+ RLEVL A +SVQLIWL+SGV+I+E I R+L +  +V+G +MF I+AFGF 
Sbjct: 121 ANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSREVNGEIMFGISAFGFF 180

Query: 181 LNLFMVMWLGHSHHSHSSHCCHHHHDNHSHSHQ-NHLDHESDDEEEIYTTTKQEDASLGS 240
           +NL MV+WLGH +HSH  H  HHHH NH H HQ +H +  +++EEE     K E +S   
Sbjct: 181 MNLVMVLWLGH-NHSHHHHDHHHHHHNHKHQHQHHHKEVVAEEEEEEMNPLKGEKSS--- 240

Query: 241 KDNSSMNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALATTF 300
             +  MNIN+QGAYLH + DMIQS+GVMI G I+W KP W++VDLICTLVFS  ALA T 
Sbjct: 241 --SKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATL 300

Query: 301 SMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAEPG 360
            +L++I GILME  PR+++IE LE  LK I GV  ++DLH+W ITVG++VLSCH++ EPG
Sbjct: 301 PILKNIFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPG 360

Query: 361 VCSRELILKIRSLCEKRYNIVHTTIQVE 388
              +E+I  +R+ C K Y I H T+QVE
Sbjct: 361 ASPKEIITGVRNFCRKSYGIYHATVQVE 373

BLAST of Lsi02G013300 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 315.8 bits (808), Expect = 6.6e-85
Identity = 172/367 (46.87%), Postives = 249/367 (67.85%), Query Frame = 0

Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAH 99
           C  + C FS  ++++ ++ +RS S  KL   +    + + VE++GG++ANSLA++TDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRI 159
           LLSDVA F++SLF++W +GWEATP+ ++G+ R+E+LGALVS+QLIWL++G+L+YE I RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 160 LGQKTKVDGFLMFSIAAFGFVLNLFMVMWLGHSH-HSHS-SHCCHHHHDNHSH-----SH 219
           + + ++V+GFLMF +AAFG V+N+ M + LGH H HSH   H   H H NHSH     +H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 220 QNHLDHE-------SDDEEEIYTTTKQE-----DASLGSKDNSSMNINLQGAYLHVITDM 279
            +H DHE        +D+   +    ++        + +K+    NINLQGAYLHV+ D 
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDS 270

Query: 280 IQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALATTFSMLRHIAGILMEETPREVNIE 339
           IQS+GVMI G I+W+ P+W IVDLICTL FSV+ L TT +M+R+I  +LME TPRE++  
Sbjct: 271 IQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDAT 330

Query: 340 NLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAEPGVCSRELILKIRSLCEKRYNIV 388
            LE  L  ++ V  +H+LHIW+ITVGKV+L+CHV   P   +  ++ K+     + YNI 
Sbjct: 331 KLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNIS 390

BLAST of Lsi02G013300 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.8e-83
Identity = 180/413 (43.58%), Postives = 260/413 (62.95%), Query Frame = 0

Query: 15  HEVKMPIVAKKR-NVSIPNSPGQEVKCC-SSGCAFSRLEHSNLESLKRSKSAMKLGGLIF 74
           H    P +A+ R ++S   S     K C  + C FS   +S+ ++ +R  S  KL   + 
Sbjct: 4   HNSAPPQIAEVRMDISSSTSVAAGNKVCRGAACDFSDSSNSSKDARERMASMRKLIIAVI 63

Query: 75  FYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQYSFGYNRL 134
              I + VE++GG++ANSLA++TDAAHLLSDVA F++SLF++W +GWEATPQ S+G+ R+
Sbjct: 64  LCIIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPQQSYGFFRI 123

Query: 135 EVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFVLNLFMVMWLGHS 194
           E+LGALVS+QLIWL++G+L+YE I R++ +  +V G LMF+++AFG  +N+ M + LGH 
Sbjct: 124 EILGALVSIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFVNIIMAVLLGHD 183

Query: 195 H-----------HSH-----SSHCCHHHHDNHSHSHQNHLD------------------- 254
           H           HSH      S   HHHH++  H H +H +                   
Sbjct: 184 HGHGHGHGHGHGHSHDHDHGGSDHDHHHHEDQEHGHVHHHEDGHGNSITVNLHHHPGTGH 243

Query: 255 HESDDEEEIYTTTKQEDAS-LGSKD--NSSMNINLQGAYLHVITDMIQSIGVMIAGVILW 314
           H  D EE +  +    D++  G+KD   +  NIN+  AYLHV+ D IQSIGVMI G I+W
Sbjct: 244 HHHDAEEPLLKSDAGCDSTQSGAKDAKKARRNINVHSAYLHVLGDSIQSIGVMIGGAIIW 303

Query: 315 FKPDWIIVDLICTLVFSVLALATTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHY 374
           +KP+W I+DLICTL+FSV+ L TT  MLR+I  +LME TPRE++  +LEN L+++ GV  
Sbjct: 304 YKPEWKIIDLICTLIFSVIVLFTTIKMLRNILEVLMESTPREIDATSLENGLRDMDGVVA 363

Query: 375 LHDLHIWSITVGKVVLSCHVIAEPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           +H+LHIW+ITVGKV+L+CHV       + +++ K+    +  YNI H TIQ+E
Sbjct: 364 VHELHIWAITVGKVLLACHVTITQDADADQMLDKVIGYIKSEYNISHVTIQIE 416

BLAST of Lsi02G013300 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 303.5 bits (776), Expect = 3.4e-81
Identity = 172/382 (45.03%), Postives = 250/382 (65.45%), Query Frame = 0

Query: 10  ITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKRSKSAMKLGG 69
           I EH H V++         S+    G +  C  + C FS  + S++E+ +R+ S  KL  
Sbjct: 20  IHEHDHMVQICGEVSSGETSL---VGIKKTCGEAPCGFSDAKTSSIEAQERAASMRKLLI 79

Query: 70  LIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQYSFGY 129
            +    I IVVE++GG++ANSLA++TDAAHLLSDVA F++SLF++W SGW+A PQ S+G+
Sbjct: 80  AVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGF 139

Query: 130 NRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFVLNLFMVMWL 189
            R+E+LGALVS+Q+IWL++G+L+YE I R+     +V+G LMF+++A G ++N+ M + L
Sbjct: 140 FRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILL 199

Query: 190 GHSH-HSHSSHCCHHHHDN---HSHSHQNHLDHESDDEEEIYTTTKQEDASLGSKDNSSM 249
           GH H H H      H HDN   HSH H + +       +  +  ++  D  +  K     
Sbjct: 200 GHDHGHGHG-----HSHDNGHGHSHDHGHGIAATEHHHDSGHDESQLSDVLIEQK--KQR 259

Query: 250 NINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALATTFSMLRHI 309
           N+N+QGAYLHV+ D IQS+GVMI G I+W+KP+W I+DLICTLVFSV+ L TT  MLR+I
Sbjct: 260 NVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNI 319

Query: 310 AGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAEPGVCSREL 369
             +LME TPRE++   LE  +  I+ V  +H+LHIW+ITVGK++L+CHV   P   +  +
Sbjct: 320 LEVLMESTPREIDPTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMV 379

Query: 370 ILKIRSLCEKRYNIVHTTIQVE 388
           + KI    ++ +NI H TIQ+E
Sbjct: 380 LDKIIDYIKREHNISHVTIQIE 391

BLAST of Lsi02G013300 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 6.4e-72
Identity = 151/350 (43.14%), Postives = 219/350 (62.57%), Query Frame = 0

Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAH 99
           C  + C FS    S+ ++ KR+ S  KL  ++    + + +E++ G++ANSLA++ DAAH
Sbjct: 9   CGETACGFST---SSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILADAAH 68

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRI 159
           LL+DV  F++S+ ++W S WEA P+ S+G+ R+E+LG LVS+QLIWL++G+L+YE + R+
Sbjct: 69  LLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEAVTRL 128

Query: 160 LGQ-KTKVDGFLMFSIAAFGFVLNLFMVMWLGHSH-HSHSSHCCHHHHDNHSHSHQNHLD 219
           + +    VDGF M  +AAFG V+N+ M++ LGH H H H     H H  +H HS+    +
Sbjct: 129 VQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHD----HGHSHDHGHSYGERAE 188

Query: 220 HESDDEEEIYTTTKQEDASLGSKDNSSMNINLQGAYLHVITDMIQSIGVMIAGVILWFKP 279
              +  +EI                   NIN+QGAYLHV+ D+IQSIGVMI G ++W+ P
Sbjct: 189 QLLEKSKEI------------------RNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNP 248

Query: 280 DWIIVDLICTLVFSVLALATTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHD 339
            W ++DLICTL FSV+ L TT  MLR I  +LME TPRE++   LE  L  I+ V  +H+
Sbjct: 249 KWKVIDLICTLFFSVIVLGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHE 308

Query: 340 LHIWSITVGKVVLSCHVIAEPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           LHIW+ITVGK + SCHV   P      ++ K+     + Y I H TIQ+E
Sbjct: 309 LHIWAITVGKALFSCHVKVRPEAGDEMVLNKVIDYIWREYRISHVTIQIE 333

BLAST of Lsi02G013300 vs. ExPASy TrEMBL
Match: A0A1S3C537 (metal tolerance protein B OS=Cucumis melo OX=3656 GN=LOC103497126 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 4.3e-180
Identity = 342/392 (87.24%), Postives = 361/392 (92.09%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           M EE+V IL TEHW EVKMPIVAKKRNV IP S   EVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIPTS--SEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+I FYTIAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
           ATPQ+SFGYNRLEVLGALVSVQLIWLISGVLIYE IDRIL QKTKVDGFLMF++AAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVMWLGHS---HHSHSSHCCHHHHDNHSHSHQNHLDHESDDE-EEIYTTTKQEDAS 240
           LNLFMV+WLGHS   HHSHSSHCCHH H +HSHS QNHL HESD E EEIYT TK E AS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLAL 300
           LGSKDNSS +NINLQGAYLHVITDMIQSIGVMIAG +LWFKP+WI+VDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVI 360
           ATTFSMLRHIA ILME TPREV+IE+LEND+KNIKGVH LHDLHIWSITVGKVVLSCHV+
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           AE GVCSRELILKI+S CEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

BLAST of Lsi02G013300 vs. ExPASy TrEMBL
Match: A0A5D3BGQ9 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001790 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 4.3e-180
Identity = 342/392 (87.24%), Postives = 361/392 (92.09%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           M EE+V IL TEHW EVKMPIVAKKRNV IP S   EVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIPTS--SEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+I FYTIAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
           ATPQ+SFGYNRLEVLGALVSVQLIWLISGVLIYE IDRIL QKTKVDGFLMF++AAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVMWLGHS---HHSHSSHCCHHHHDNHSHSHQNHLDHESDDE-EEIYTTTKQEDAS 240
           LNLFMV+WLGHS   HHSHSSHCCHH H +HSHS QNHL HESD E EEIYT TK E AS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLAL 300
           LGSKDNSS +NINLQGAYLHVITDMIQSIGVMIAG +LWFKP+WI+VDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVI 360
           ATTFSMLRHIA ILME TPREV+IE+LEND+KNIKGVH LHDLHIWSITVGKVVLSCHV+
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           AE GVCSRELILKI+S CEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

BLAST of Lsi02G013300 vs. ExPASy TrEMBL
Match: A0A5A7SNE8 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00010 PE=4 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 8.1e-179
Identity = 339/392 (86.48%), Postives = 359/392 (91.58%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           M EE+V IL TEHW EVKMPIVAKKRNV IP S   EVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIPTS--SEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+I FYTIAIVVEIIGG RANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
            TPQ+SFGYNRLEVLGALVSVQLIWLISGVLIYE IDRIL QKTKVDGFLMF++AAFGF+
Sbjct: 121 VTPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVMWLGHS---HHSHSSHCCHHHHDNHSHSHQNHLDHESDDE-EEIYTTTKQEDAS 240
           LNLFMV+WLGHS   HHSHSSHCCHH H +HSHS QNHL HESD E EEIYT TK E AS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLAL 300
           LGSKDNSS +NINLQGAYLHVITD+IQSIGVMIAG +LWFKP+WI+VDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDLIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVI 360
           ATTFSMLRHIA ILME TPREV+IE+LEND+KNIKGVH LHDLHIWSITVGKVVLSCHV+
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           AE GVCSRELILKI+S CEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

BLAST of Lsi02G013300 vs. ExPASy TrEMBL
Match: I1ZI47 (Metal transport protein 4 OS=Cucumis sativus OX=3659 GN=MTP4 PE=2 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.1e-175
Identity = 332/391 (84.91%), Postives = 357/391 (91.30%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           M EE+V IL TEH  E+ +PIVAKK N  IP S   EVKCCSSGCAFSRLEHSNLESLKR
Sbjct: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGGLI FYTIAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
           ATPQ+SFGYNRLEVLGALVSVQLIWLISG+LIYE IDRIL  KTKVDGFLMF++AAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180

Query: 181 LNLFMVMWLGHS---HHSHSSHCCHHHHDNHSHSHQNHLDHESDDEEEIYTTTKQEDASL 240
           LNLFMV+WLGHS   HHSHSSHCC  HHD+HSHSHQNHL+HE   EEE+YT TKQE ASL
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCC--HHDHHSHSHQNHLEHE---EEEVYTLTKQEGASL 240

Query: 241 GSKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALA 300
           GSKDNSS +NINLQGAYLHVITDMIQSIGVMIAG++LWFKP+WI+VDLICTLVFSVLALA
Sbjct: 241 GSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALA 300

Query: 301 TTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIA 360
           TTFSMLRH A ILME TPREV+IE+LEND+KN+KGV+ LHDLHIWSITVGKVVLSCHV+A
Sbjct: 301 TTFSMLRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVA 360

Query: 361 EPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           E GVCSRELILKI+S CEKRYNIVHTTIQVE
Sbjct: 361 EAGVCSRELILKIKSHCEKRYNIVHTTIQVE 386

BLAST of Lsi02G013300 vs. ExPASy TrEMBL
Match: A0A0A0M0L3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525270 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.2e-175
Identity = 331/391 (84.65%), Postives = 356/391 (91.05%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           M EE+V IL TEH  E+ +PIVAKK N  IP S   EVKCCSSGCAFSRLEHSNLESLKR
Sbjct: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGGLI FYTIAIVVEIIGG RANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGLILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
           ATPQ+SFGYNRLEVLGALVSVQLIWLISG+LIYE IDRIL  KTKVDGFLMF++AAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180

Query: 181 LNLFMVMWLGHS---HHSHSSHCCHHHHDNHSHSHQNHLDHESDDEEEIYTTTKQEDASL 240
           LNLFMV+WLGHS   HHSHSSHCC  HHD+HSHSHQNHL+HE   EEE+YT TKQE ASL
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCC--HHDHHSHSHQNHLEHE---EEEVYTLTKQEGASL 240

Query: 241 GSKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALA 300
           GSKDNSS +NINLQGAYLHVITDMIQSIGVMIAG++LWFKP+WI+VDLICTLVFSVLALA
Sbjct: 241 GSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALA 300

Query: 301 TTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIA 360
           TTFSMLRH A ILME TPREV+IE+LEND+KN+KGV+ LHDLHIWSITVGKVVLSCHV+A
Sbjct: 301 TTFSMLRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVA 360

Query: 361 EPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           E GVCSRELILKI+S CEKRYNIVHTTIQVE
Sbjct: 361 EAGVCSRELILKIKSHCEKRYNIVHTTIQVE 386

BLAST of Lsi02G013300 vs. NCBI nr
Match: TYJ97368.1 (metal tolerance protein B [Cucumis melo var. makuwa])

HSP 1 Score: 640.6 bits (1651), Expect = 8.9e-180
Identity = 342/392 (87.24%), Postives = 361/392 (92.09%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           M EE+V IL TEHW EVKMPIVAKKRNV IP S   EVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIPTS--SEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+I FYTIAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
           ATPQ+SFGYNRLEVLGALVSVQLIWLISGVLIYE IDRIL QKTKVDGFLMF++AAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVMWLGHS---HHSHSSHCCHHHHDNHSHSHQNHLDHESDDE-EEIYTTTKQEDAS 240
           LNLFMV+WLGHS   HHSHSSHCCHH H +HSHS QNHL HESD E EEIYT TK E AS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLAL 300
           LGSKDNSS +NINLQGAYLHVITDMIQSIGVMIAG +LWFKP+WI+VDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVI 360
           ATTFSMLRHIA ILME TPREV+IE+LEND+KNIKGVH LHDLHIWSITVGKVVLSCHV+
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           AE GVCSRELILKI+S CEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

BLAST of Lsi02G013300 vs. NCBI nr
Match: XP_008457438.1 (PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457439.1 PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457440.1 PREDICTED: metal tolerance protein B [Cucumis melo])

HSP 1 Score: 640.6 bits (1651), Expect = 8.9e-180
Identity = 342/392 (87.24%), Postives = 361/392 (92.09%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           M EE+V IL TEHW EVKMPIVAKKRNV IP S   EVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIPTS--SEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+I FYTIAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
           ATPQ+SFGYNRLEVLGALVSVQLIWLISGVLIYE IDRIL QKTKVDGFLMF++AAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVMWLGHS---HHSHSSHCCHHHHDNHSHSHQNHLDHESDDE-EEIYTTTKQEDAS 240
           LNLFMV+WLGHS   HHSHSSHCCHH H +HSHS QNHL HESD E EEIYT TK E AS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLAL 300
           LGSKDNSS +NINLQGAYLHVITDMIQSIGVMIAG +LWFKP+WI+VDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVI 360
           ATTFSMLRHIA ILME TPREV+IE+LEND+KNIKGVH LHDLHIWSITVGKVVLSCHV+
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           AE GVCSRELILKI+S CEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

BLAST of Lsi02G013300 vs. NCBI nr
Match: KAA0031763.1 (metal tolerance protein B [Cucumis melo var. makuwa])

HSP 1 Score: 636.3 bits (1640), Expect = 1.7e-178
Identity = 339/392 (86.48%), Postives = 359/392 (91.58%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           M EE+V IL TEHW EVKMPIVAKKRNV IP S   EVKCC+SGCAFSRLEHSNLESLKR
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIPTS--SEVKCCNSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGG+I FYTIAIVVEIIGG RANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGMILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
            TPQ+SFGYNRLEVLGALVSVQLIWLISGVLIYE IDRIL QKTKVDGFLMF++AAFGF+
Sbjct: 121 VTPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFI 180

Query: 181 LNLFMVMWLGHS---HHSHSSHCCHHHHDNHSHSHQNHLDHESDDE-EEIYTTTKQEDAS 240
           LNLFMV+WLGHS   HHSHSSHCCHH H +HSHS QNHL HESD E EEIYT TK E AS
Sbjct: 181 LNLFMVIWLGHSHHHHHSHSSHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGAS 240

Query: 241 LGSKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLAL 300
           LGSKDNSS +NINLQGAYLHVITD+IQSIGVMIAG +LWFKP+WI+VDLICTLVFSVLAL
Sbjct: 241 LGSKDNSSTLNINLQGAYLHVITDLIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLAL 300

Query: 301 ATTFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVI 360
           ATTFSMLRHIA ILME TPREV+IE+LEND+KNIKGVH LHDLHIWSITVGKVVLSCHV+
Sbjct: 301 ATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVV 360

Query: 361 AEPGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           AE GVCSRELILKI+S CEKRYNIVHTTIQVE
Sbjct: 361 AEAGVCSRELILKIKSHCEKRYNIVHTTIQVE 390

BLAST of Lsi02G013300 vs. NCBI nr
Match: XP_038893887.1 (metal tolerance protein B [Benincasa hispida] >XP_038893888.1 metal tolerance protein B [Benincasa hispida])

HSP 1 Score: 636.0 bits (1639), Expect = 2.2e-178
Identity = 338/390 (86.67%), Postives = 359/390 (92.05%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQ-EVKCCSSGCAFSRLEHSNLESLK 60
           M EE+V IL TE+W EV MPIV KKRNVSIP  P   EVKCCSSGCAFSRLEH NLESLK
Sbjct: 1   MGEEEVLILKTENWDEVMMPIVPKKRNVSIPIIPNSPEVKCCSSGCAFSRLEHCNLESLK 60

Query: 61  RSKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGW 120
           RSKSAMKLGGLI FYTIAIVVEIIGGLRANSLAVMTDA HLLSDVAGF+ SLFAVWVSGW
Sbjct: 61  RSKSAMKLGGLILFYTIAIVVEIIGGLRANSLAVMTDAVHLLSDVAGFAASLFAVWVSGW 120

Query: 121 EATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGF 180
           EATPQ+SFGYNRLEVLGALVSVQLIWLISGVLIY+ I+RILGQKTKVDGFLMF+IAAFGF
Sbjct: 121 EATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYQAINRILGQKTKVDGFLMFAIAAFGF 180

Query: 181 VLNLFMVMWLGHSHHSHSSHCCHHHHDNHSHSHQNHLDHESDDE-EEIYTTTKQEDASLG 240
           +LNLFMV+WLGHSH SHSSHCC  HHD+HSHSHQ+HLDHESD E EEIYT TKQE  SL 
Sbjct: 181 ILNLFMVIWLGHSHDSHSSHCC--HHDHHSHSHQHHLDHESDHENEEIYTLTKQEGGSLA 240

Query: 241 SKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALAT 300
           S+ NSS ++IN+QGAYLHVITDMIQSIGVMIAGVILWFKPDWI+VDLICTLVFSVL+LAT
Sbjct: 241 SQGNSSTLDINIQGAYLHVITDMIQSIGVMIAGVILWFKPDWIVVDLICTLVFSVLSLAT 300

Query: 301 TFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAE 360
           TFSMLRH AGILME TP+EVNIE LENDLKNIKGVH LHDLHIWSITVGKVVLSCHV+AE
Sbjct: 301 TFSMLRHTAGILMEGTPKEVNIEILENDLKNIKGVHDLHDLHIWSITVGKVVLSCHVVAE 360

Query: 361 PGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
           PGVC+RELILKIR+LCEKRYNIVHTTIQVE
Sbjct: 361 PGVCTRELILKIRNLCEKRYNIVHTTIQVE 388

BLAST of Lsi02G013300 vs. NCBI nr
Match: XP_031741134.1 (metal tolerance protein B-like [Cucumis sativus] >XP_031741135.1 metal tolerance protein B-like [Cucumis sativus] >KAE8653268.1 hypothetical protein Csa_023325 [Cucumis sativus])

HSP 1 Score: 626.3 bits (1614), Expect = 1.7e-175
Identity = 332/390 (85.13%), Postives = 357/390 (91.54%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           M EE+V IL TEH  E+ +PIVAKK N  IP S   EVKCCSSGCAFSRLEHSNLESLKR
Sbjct: 1   MGEEEVLILKTEHSDEINIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKR 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
           SKSAMKLGGLI FYTIAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGWE
Sbjct: 61  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
           ATPQ+SFGYNRLEVLGALVSVQLIWLISG+LIYE IDRIL  KTKVDGFLMF++AAFGF+
Sbjct: 121 ATPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFL 180

Query: 181 LNLFMVMWLGHS--HHSHSSHCCHHHHDNHSHSHQNHLDHESDDEEEIYTTTKQEDASLG 240
           LNLFMV+WLGHS  HHSHSSHCC  HHD+HSHSHQNHL+HE   EEE+YT TKQE ASLG
Sbjct: 181 LNLFMVIWLGHSHHHHSHSSHCC--HHDHHSHSHQNHLEHE---EEEVYTLTKQEGASLG 240

Query: 241 SKDNSS-MNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALAT 300
           SKDNSS +NINLQGAYLHVITDMIQSIGVMIAG++LWFKP+WI+VDLICTLVFSVLALAT
Sbjct: 241 SKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALAT 300

Query: 301 TFSMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAE 360
           TFSMLRH A ILME TPREV+IE+LEND+KN+KGV+ LHDLHIWSITVGKVVLSCHV+AE
Sbjct: 301 TFSMLRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAE 360

Query: 361 PGVCSRELILKIRSLCEKRYNIVHTTIQVE 388
            GVCSRELILKI+S CEKRYNIVHTTIQVE
Sbjct: 361 AGVCSRELILKIKSHCEKRYNIVHTTIQVE 385

BLAST of Lsi02G013300 vs. TAIR 10
Match: AT2G29410.1 (metal tolerance protein B1 )

HSP 1 Score: 370.2 bits (949), Expect = 2.1e-102
Identity = 202/388 (52.06%), Postives = 266/388 (68.56%), Query Frame = 0

Query: 1   MEEEKVPILITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKR 60
           ME E++ IL  +   E++ P          P+   + +      CAF+R EH   E+ +R
Sbjct: 1   MELEQICILKPDDEEEMESP---------SPSKTEENLGVVPLSCAFTRQEHCVSETKER 60

Query: 61  SKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWE 120
            +S  +L  LIF Y I + V+I+GG +ANSLAVMTDAAHLLSDVAG  VSL A+ VS WE
Sbjct: 61  EESTRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDVAGLCVSLLAIKVSSWE 120

Query: 121 ATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFV 180
           A P+ SFG+ RLEVL A +SVQLIWL+SGV+I+E I R+L +  +V+G +MF I+AFGF 
Sbjct: 121 ANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSREVNGEIMFGISAFGFF 180

Query: 181 LNLFMVMWLGHSHHSHSSHCCHHHHDNHSHSHQ-NHLDHESDDEEEIYTTTKQEDASLGS 240
           +NL MV+WLGH +HSH  H  HHHH NH H HQ +H +  +++EEE     K E +S   
Sbjct: 181 MNLVMVLWLGH-NHSHHHHDHHHHHHNHKHQHQHHHKEVVAEEEEEEMNPLKGEKSS--- 240

Query: 241 KDNSSMNINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALATTF 300
             +  MNIN+QGAYLH + DMIQS+GVMI G I+W KP W++VDLICTLVFS  ALA T 
Sbjct: 241 --SKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATL 300

Query: 301 SMLRHIAGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAEPG 360
            +L++I GILME  PR+++IE LE  LK I GV  ++DLH+W ITVG++VLSCH++ EPG
Sbjct: 301 PILKNIFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPG 360

Query: 361 VCSRELILKIRSLCEKRYNIVHTTIQVE 388
              +E+I  +R+ C K Y I H T+QVE
Sbjct: 361 ASPKEIITGVRNFCRKSYGIYHATVQVE 373

BLAST of Lsi02G013300 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 315.8 bits (808), Expect = 4.7e-86
Identity = 172/367 (46.87%), Postives = 249/367 (67.85%), Query Frame = 0

Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAH 99
           C  + C FS  ++++ ++ +RS S  KL   +    + + VE++GG++ANSLA++TDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRI 159
           LLSDVA F++SLF++W +GWEATP+ ++G+ R+E+LGALVS+QLIWL++G+L+YE I RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 160 LGQKTKVDGFLMFSIAAFGFVLNLFMVMWLGHSH-HSHS-SHCCHHHHDNHSH-----SH 219
           + + ++V+GFLMF +AAFG V+N+ M + LGH H HSH   H   H H NHSH     +H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 220 QNHLDHE-------SDDEEEIYTTTKQE-----DASLGSKDNSSMNINLQGAYLHVITDM 279
            +H DHE        +D+   +    ++        + +K+    NINLQGAYLHV+ D 
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDS 270

Query: 280 IQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALATTFSMLRHIAGILMEETPREVNIE 339
           IQS+GVMI G I+W+ P+W IVDLICTL FSV+ L TT +M+R+I  +LME TPRE++  
Sbjct: 271 IQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDAT 330

Query: 340 NLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAEPGVCSRELILKIRSLCEKRYNIV 388
            LE  L  ++ V  +H+LHIW+ITVGKV+L+CHV   P   +  ++ K+     + YNI 
Sbjct: 331 KLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNIS 390

BLAST of Lsi02G013300 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 315.8 bits (808), Expect = 4.7e-86
Identity = 172/367 (46.87%), Postives = 249/367 (67.85%), Query Frame = 0

Query: 40  CCSSGCAFSRLEHSNLESLKRSKSAMKLGGLIFFYTIAIVVEIIGGLRANSLAVMTDAAH 99
           C  + C FS  ++++ ++ +RS S  KL   +    + + VE++GG++ANSLA++TDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 100 LLSDVAGFSVSLFAVWVSGWEATPQYSFGYNRLEVLGALVSVQLIWLISGVLIYEVIDRI 159
           LLSDVA F++SLF++W +GWEATP+ ++G+ R+E+LGALVS+QLIWL++G+L+YE I RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 160 LGQKTKVDGFLMFSIAAFGFVLNLFMVMWLGHSH-HSHS-SHCCHHHHDNHSH-----SH 219
           + + ++V+GFLMF +AAFG V+N+ M + LGH H HSH   H   H H NHSH     +H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 220 QNHLDHE-------SDDEEEIYTTTKQE-----DASLGSKDNSSMNINLQGAYLHVITDM 279
            +H DHE        +D+   +    ++        + +K+    NINLQGAYLHV+ D 
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDS 270

Query: 280 IQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALATTFSMLRHIAGILMEETPREVNIE 339
           IQS+GVMI G I+W+ P+W IVDLICTL FSV+ L TT +M+R+I  +LME TPRE++  
Sbjct: 271 IQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDAT 330

Query: 340 NLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAEPGVCSRELILKIRSLCEKRYNIV 388
            LE  L  ++ V  +H+LHIW+ITVGKV+L+CHV   P   +  ++ K+     + YNI 
Sbjct: 331 KLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKVIDYIRREYNIS 390

BLAST of Lsi02G013300 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 303.5 bits (776), Expect = 2.4e-82
Identity = 172/382 (45.03%), Postives = 250/382 (65.45%), Query Frame = 0

Query: 10  ITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKRSKSAMKLGG 69
           I EH H V++         S+    G +  C  + C FS  + S++E+ +R+ S  KL  
Sbjct: 59  IHEHDHMVQICGEVSSGETSL---VGIKKTCGEAPCGFSDAKTSSIEAQERAASMRKLLI 118

Query: 70  LIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQYSFGY 129
            +    I IVVE++GG++ANSLA++TDAAHLLSDVA F++SLF++W SGW+A PQ S+G+
Sbjct: 119 AVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGF 178

Query: 130 NRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFVLNLFMVMWL 189
            R+E+LGALVS+Q+IWL++G+L+YE I R+     +V+G LMF+++A G ++N+ M + L
Sbjct: 179 FRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILL 238

Query: 190 GHSH-HSHSSHCCHHHHDN---HSHSHQNHLDHESDDEEEIYTTTKQEDASLGSKDNSSM 249
           GH H H H      H HDN   HSH H + +       +  +  ++  D  +  K     
Sbjct: 239 GHDHGHGHG-----HSHDNGHGHSHDHGHGIAATEHHHDSGHDESQLSDVLIEQK--KQR 298

Query: 250 NINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALATTFSMLRHI 309
           N+N+QGAYLHV+ D IQS+GVMI G I+W+KP+W I+DLICTLVFSV+ L TT  MLR+I
Sbjct: 299 NVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNI 358

Query: 310 AGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAEPGVCSREL 369
             +LME TPRE++   LE  +  I+ V  +H+LHIW+ITVGK++L+CHV   P   +  +
Sbjct: 359 LEVLMESTPREIDPTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMV 418

Query: 370 ILKIRSLCEKRYNIVHTTIQVE 388
           + KI    ++ +NI H TIQ+E
Sbjct: 419 LDKIIDYIKREHNISHVTIQIE 430

BLAST of Lsi02G013300 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 303.5 bits (776), Expect = 2.4e-82
Identity = 172/382 (45.03%), Postives = 250/382 (65.45%), Query Frame = 0

Query: 10  ITEHWHEVKMPIVAKKRNVSIPNSPGQEVKCCSSGCAFSRLEHSNLESLKRSKSAMKLGG 69
           I EH H V++         S+    G +  C  + C FS  + S++E+ +R+ S  KL  
Sbjct: 20  IHEHDHMVQICGEVSSGETSL---VGIKKTCGEAPCGFSDAKTSSIEAQERAASMRKLLI 79

Query: 70  LIFFYTIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQYSFGY 129
            +    I IVVE++GG++ANSLA++TDAAHLLSDVA F++SLF++W SGW+A PQ S+G+
Sbjct: 80  AVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGF 139

Query: 130 NRLEVLGALVSVQLIWLISGVLIYEVIDRILGQKTKVDGFLMFSIAAFGFVLNLFMVMWL 189
            R+E+LGALVS+Q+IWL++G+L+YE I R+     +V+G LMF+++A G ++N+ M + L
Sbjct: 140 FRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILL 199

Query: 190 GHSH-HSHSSHCCHHHHDN---HSHSHQNHLDHESDDEEEIYTTTKQEDASLGSKDNSSM 249
           GH H H H      H HDN   HSH H + +       +  +  ++  D  +  K     
Sbjct: 200 GHDHGHGHG-----HSHDNGHGHSHDHGHGIAATEHHHDSGHDESQLSDVLIEQK--KQR 259

Query: 250 NINLQGAYLHVITDMIQSIGVMIAGVILWFKPDWIIVDLICTLVFSVLALATTFSMLRHI 309
           N+N+QGAYLHV+ D IQS+GVMI G I+W+KP+W I+DLICTLVFSV+ L TT  MLR+I
Sbjct: 260 NVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNI 319

Query: 310 AGILMEETPREVNIENLENDLKNIKGVHYLHDLHIWSITVGKVVLSCHVIAEPGVCSREL 369
             +LME TPRE++   LE  +  I+ V  +H+LHIW+ITVGK++L+CHV   P   +  +
Sbjct: 320 LEVLMESTPREIDPTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMV 379

Query: 370 ILKIRSLCEKRYNIVHTTIQVE 388
           + KI    ++ +NI H TIQ+E
Sbjct: 380 LDKIIDYIKREHNISHVTIQIE 391