Lsi02G013160 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G013160
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA-directed RNA polymerases I and III subunit RPAC2
Location	chr02: 17606864 .. 17610490 (-)
RNA-Seq Expression	Lsi02G013160
Synteny	Lsi02G013160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTCAGCTCCCTTTTAGTTTTTGTGATCTCTCCCGCCCTCTGTGCTCTCTCCATCCTCCTACAACTCCCCGGCCGGCCGGCCACCGACGGTCGCAACTCGATCTCTTCTCGTCCTCCTTCTCTCTCTCGGTAATCACTTCTCACGCTTGCGCTCTCTCTCTCTCGCTATATATATATATGTATGTATGTATATGGTCAGTTCGCCCATCAACAAGTGAGCGATGCGAATGTTTGTTGCTTATTCCCCGTGGGTGTTTAAGCATTATCTGGGTTCGTCCTTGTTGAATTGACTGATTTTTGTTGTTTTAGTTATGAAGAATCTATAATTGAATTTGTGTCAAAAGATGGGTATTTCAAACCATCTAATTCTAGGTTATTTTGTGGACGTAATTTTCTTTATGTTTATGGGTACGGTGTAGTTATTTTGGTTAGTTGATTTAATTTTTGGTGGTTTACTGGAAAACTTGTTTAGTCATGTCGCTTGGAAACTAAAGAATACTGAATTGATCTGAAATTTTAGTTACACATCAGTACTGTCCTATAAGGGAGAATTAGAATATATGAAGGAGGCCCAATTCTGCATGCCCAAAACCTAAAAACTGAACTGAATGCACTCCACTAATGCGTGAGCTCATGTATTAGTTTCGATTTGCAATATCTAAATGTTAGCGTGAAAGAGGTGATGATACTATAGGAGTTAGCTTAAGATCTCTTAAATTGTAAGATAAGAGCAAATCATGTCAATTTGTTCCTTAGAACTTTTTAGATTAAAAACCTAATAGGTATCCTTCATGTAAAAGTGATGCCTTGCATCATGGTCTTCTTCTCTTCTTACTAAAATCATTTCTCGAGGTGCCCCGTGGAACTAGACGTGGGACTCTAGCTCTAAATTCAAATACTTACTGTGTAAGAGTAAGTAATGCAAGGAGTAATTGCAATTGATAGGAAAGAATGCAATGCAAGGAGTAATTGTACGTTTATTGCTCAATAGCACACTCTAAACATCTTCATACTCAGGGCCTAATACTTCAATGGCTTCTCCTACTACTAGCCCATGCCTCTTTTAGGAGTAACATCTGGAGAAGTAAAGCTGGTGAGGATAATAGTAATATAGTCTCGAAGAAATAACTTATCAAAAGTTTCCTCTTCAAGCTACTTATATGTATGTATTATAGATTAGAGGTTTCTGGGGATAATTGAATAATCATGAAATTAGTCTAGCTAATTTTATGTGCCTGCACTTCTGTTAACTCCCGAAGATAGATATTTCAATTCTCAAGAGTTGGTGGAAAAGAGATTTCAACTTCTTGGCTCCGTTGGTTTTGTAGAATTTTGGCTGATTTTTTTTCGGTTTATTTTCCTTGATCTTCTCAATTTATTTGCCTTCTGAATGCTGATTCTGTTTGGTTTTTCATTTTTTTTATAGCAATGGAACATGGATCCTTCACAGATGAGACCAAATCAACATTTTCAATGGCTGATGAAGATCATACACTTGCAAATGCTCTCAGATACACTTTGAATCAAGAGTAGGAGCTCTTATTCAGTTATTCTTGATTGAATATTTTGAAATGTTAACAACGCATTCACATGCCAAAGAATTTGCAGTTCTTGGACTACTTATCTTATCTAACCATTTCATCATTGACTTCTATTAATTCATGACATAAAGAATTAAATAGATTATGTTCTTGTGAACATATCATTAATAAGGTCCTTGAAGAACTAATTAGGTCCTTGGAGAACCCATGATGCAGAAATTGATAAATAATGCAAAACCACTGCATGTGTTAATAATAATATGGGTTCTTTCATTGTCCATTCAGAAGAGTTATAAACAGTGTTGTCATCTTTTTTCACCTATTCAAGCAAGAGGAATTGATAATTTGGCTGTTTTCTTTTTCCTTCTTAAAAAAGCTGGTTTAGATTGTTGTTGTTCTTTAGTAAATTATATCTGACATACTTTTAAATGACATAACATCATATTGCAGTCCAAGAGTGACATTCTGTGGGTACAGCATCCCTCATCCTTCAGATAATCGAGTTAATATTAGAGTTCAGACAACAGGTAATATTTTGAGGTAGTTTGATTGTCTGAAGTGTAACTGATTGGTTCCTATTTAGGACATTGTTTTTAATGATTAGATTTGTTCTTTTCTGGTTTTTCTTCTTTACACACATCCAATTCTTGGATTCATGAGATATATAGCTTATGAATTTATGCGAAGTGTCATCCTTCAAAAAGAAAAATCTTTTGATAAAATTGTTTTTTTTTTTTTAAGAACATAGGTTGGATCCTTGTTGCTTTGCTAGATGTAGTTCCCCATTATTTTTATAAATATAAAAATATATATATATTAGTACTTCAACAAAGGGTCGTGAAGTTTCATAACTTTAACAAGATTCAATCATGTGCCTAAAGTTTGAAAAGCTATTGTACGTAATTTTTACCATCAACAATTATAACTCAACACTGATGTGATAAATATGTTAGAGAAACATTAGTTGATTTGACATAACGTGACAGGAAAATAATAGGAAATGGATAACTATAGAAAATAAAAGGTCGTAGCACGTAAAAGAGATTTGATATGTTGTTTTGAAAAGATCATATGTTGCATATGCCAATTTATTATATTTTGTTATCCACTTTTTTCTGCAATGATATATGTGAATCAATCAATATTTGTTCGATAGGACATGTCATCACACTATTGTCTAGTAAATAATGTTAATAACTATGACTATACCTGATTAATTATACTTTAAAGTTTGAAGGCCATATTTGAAACTTTTTAAAATGAAAAATACCTATTAGGCACAACTATCAAGTTACTTTAAAACTCAAAGACATAGTTATACAACCTATAGAATAGATCCTATTTAAGTAGGGTCATAAATTTATAGTTTAAACTTGAACATATGTACATACCCTTTTGCAATTGTGAAGTAAAACCCAACCAACCAAGATGTTTGAGGTTGAGGCCTAGACAAATAGTGTCCCAAATGAATGGTCTTTACTCTTTCCCCATAGTTGGAGTAGGGGAACCCAATGATCTGCAGTTGAAAGTAGGCAAAGCTATTATTGATTACTAGGGAAAAGCAAAGCACATGGAAGTGGTAGGGGCTATTTGTTGAATAAAGCACATGCAAAGCAAAGCACAGCAGGCTATTTGTTGAAGTGACCATGCATTAGTAGCATTAGAATTAGCATTAGAGTCATACTGATGAAAGAGTTTTTTGATTGAACATGGCAGGTGATCCTGCAAGAGAGGTTTTGAAAGATGCTTGTCAGAACTTGATAGTGGTGTGCCAGCATGTGAGGAACACATTTGACAAGGCTGTGGATATCAAACTCAACAACACTGTGGGAGACATTAATATCAAATAGAATGGGACCTTTCTTTTTCTTTGGCTTGTAATTTACTTTTTTTTTTCCTTTTGTTCAATACTTATTATAGTTTACAGTTTATTGAGATGGGTTAGTTACTGGCTTTTGGAACAGATTCATTCCATTGAATTTTGCTTACTTTTACTGTTATTATCTCAACTGATGGCTGATGCCCCTCTCAGGGATTTTACGTTAAATATTAGATATTGTTGATTTTACATCTTATTAGAAGAAT

mRNA sequence

CATTCAGCTCCCTTTTAGTTTTTGTGATCTCTCCCGCCCTCTGTGCTCTCTCCATCCTCCTACAACTCCCCGGCCGGCCGGCCACCGACGGTCGCAACTCGATCTCTTCTCGTCCTCCTTCTCTCTCTCGCAATGGAACATGGATCCTTCACAGATGAGACCAAATCAACATTTTCAATGGCTGATGAAGATCATACACTTGCAAATGCTCTCAGATACACTTTGAATCAAGATCCAAGAGTGACATTCTGTGGGTACAGCATCCCTCATCCTTCAGATAATCGAGTTAATATTAGAGTTCAGACAACAGGTGATCCTGCAAGAGAGGTTTTGAAAGATGCTTGTCAGAACTTGATAGTGGTGTGCCAGCATGTGAGGAACACATTTGACAAGGCTGTGGATATCAAACTCAACAACACTGTGGGAGACATTAATATCAAATAGAATGGGACCTTTCTTTTTCTTTGGCTTGTAATTTACTTTTTTTTTTCCTTTTGTTCAATACTTATTATAGTTTACAGTTTATTGAGATGGGTTAGTTACTGGCTTTTGGAACAGATTCATTCCATTGAATTTTGCTTACTTTTACTGTTATTATCTCAACTGATGGCTGATGCCCCTCTCAGGGATTTTACGTTAAATATTAGATATTGTTGATTTTACATCTTATTAGAAGAAT

Coding sequence (CDS)

ATGGAACATGGATCCTTCACAGATGAGACCAAATCAACATTTTCAATGGCTGATGAAGATCATACACTTGCAAATGCTCTCAGATACACTTTGAATCAAGATCCAAGAGTGACATTCTGTGGGTACAGCATCCCTCATCCTTCAGATAATCGAGTTAATATTAGAGTTCAGACAACAGGTGATCCTGCAAGAGAGGTTTTGAAAGATGCTTGTCAGAACTTGATAGTGGTGTGCCAGCATGTGAGGAACACATTTGACAAGGCTGTGGATATCAAACTCAACAACACTGTGGGAGACATTAATATCAAATAG

Protein sequence

MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLKDACQNLIVVCQHVRNTFDKAVDIKLNNTVGDINIK

Homology

[Close]

BLAST of Lsi02G013160 vs. ExPASy Swiss-Prot
Match: Q5XK67 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Xenopus laevis OX=8355 GN=polr1d PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.9e-17
Identity = 40/83 (48.19%), Postives = 57/83 (68.67%), Query Frame = 0


Query: 8   DETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQT-TGDPAREV 67
           +E+  TF + +EDHTL N+LRY + ++P V FCGYSI HPS+ ++N R+QT  G PA E 
Sbjct: 20  EESCVTFVLHEEDHTLGNSLRYMVMKNPEVEFCGYSITHPSETKINFRIQTRNGIPAVEP 79

Query: 68  LKDACQNLIVVCQHVRNTFDKAV 90
            +     L+ VCQHV NTF+ ++
Sbjct: 80  FRRGLNELVDVCQHVLNTFETSI 102

[Close]

BLAST of Lsi02G013160 vs. ExPASy Swiss-Prot
Match: Q6DRI4 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Danio rerio OX=7955 GN=polr1d PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.1e-15
Identity = 40/80 (50.00%), Postives = 54/80 (67.50%), Query Frame = 0


Query: 8  DETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQT-TGDPAREV 67
          DE   TF + +EDHTL N+LRY + +   V FCGYSI HPS++++N R+QT  G PA E 
Sbjct: 19 DEGCVTFVLNEEDHTLGNSLRYMIMKSQDVEFCGYSITHPSESKINFRIQTRDGVPASEP 78

Query: 68 LKDACQNLIVVCQHVRNTFD 87
          L++   NL  VC+HV  TF+
Sbjct: 79 LRNGLNNLTDVCKHVLQTFE 98

[Close]

BLAST of Lsi02G013160 vs. ExPASy Swiss-Prot
Match: Q54ES6 (DNA-directed RNA polymerases I and III subunit rpc19 OS=Dictyostelium discoideum OX=44689 GN=rpc19 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.1e-15
Identity = 39/80 (48.75%), Postives = 55/80 (68.75%), Query Frame = 0


Query: 12  STFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGD-PAREVLKDA 71
           +TF   DEDHTL NAL Y L ++P+V F GYSIPHPSDNR+N+R+QT  +  A+E L   
Sbjct: 36  ATFIFTDEDHTLGNALHYVLMKNPKVDFSGYSIPHPSDNRMNLRIQTKSNITAQESLLQG 95

Query: 72  CQNLIVVCQHVRNTFDKAVD 91
             ++  + +H+  TF+ A+D
Sbjct: 96  LTDIKDISRHIFETFNNALD 115

[Close]

BLAST of Lsi02G013160 vs. ExPASy Swiss-Prot
Match: P28000 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPC19 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.5e-15
Identity = 38/83 (45.78%), Postives = 58/83 (69.88%), Query Frame = 0


Query: 8   DETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGD-PAREV 67
           D T ++F + +EDHTL NALRY + ++P V FCGYSIPHPS+N +NIR+QT G+  A + 
Sbjct: 57  DGTSASFQIVEEDHTLGNALRYVIMKNPDVEFCGYSIPHPSENLLNIRIQTYGETTAVDA 116

Query: 68  LKDACQNLIVVCQHVRNTFDKAV 90
           L+   ++L+ +C  V + F + +
Sbjct: 117 LQKGLKDLMDLCDVVESKFTEKI 139

[Close]

BLAST of Lsi02G013160 vs. ExPASy Swiss-Prot
Match: P0DPB6 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Homo sapiens OX=9606 GN=POLR1D PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 4.2e-15
Identity = 38/90 (42.22%), Postives = 58/90 (64.44%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           M   + TD    TF + +EDHTL N+LRY + ++P V FCGY+  HPS++++N+R+QT G
Sbjct: 29  MVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRG 88

Query: 61  D-PAREVLKDACQNLIVVCQHVRNTFDKAV 90
             PA E  +     L+ VCQHV + F+ ++
Sbjct: 89  TLPAVEPFQRGLNELMNVCQHVLDKFEASI 118

[Close]

BLAST of Lsi02G013160 vs. ExPASy TrEMBL
Match: A0A0A0LUT5 (RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G095010 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.0e-47
Identity = 96/104 (92.31%), Postives = 99/104 (95.19%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFS+ADEDHTLAN LRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNTVGDINIK 104
           DPAREVLKDACQNL+VVCQHVRNTFDKAV D+KL N V DINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDVKLTNPVRDINIK 104

[Close]

BLAST of Lsi02G013160 vs. ExPASy TrEMBL
Match: A0A6J1KDK3 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita maxima OX=3661 GN=LOC111493371 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.1e-45
Identity = 93/104 (89.42%), Postives = 97/104 (93.27%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDE+KSTFSMADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNTVGDINIK 104
           DPAREVLKDACQNL+ VCQHVRNT DKAV D KL   VGDINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

[Close]

BLAST of Lsi02G013160 vs. ExPASy TrEMBL
Match: A0A6J1D9Q8 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Momordica charantia OX=3673 GN=LOC111018936 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.1e-45
Identity = 93/104 (89.42%), Postives = 98/104 (94.23%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSF+DE+KSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFSDESKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNTVGDINIK 104
           D AREVLKD+CQNL+VVCQHVRNTFDK+V D KL   VGDINIK
Sbjct: 61  DSAREVLKDSCQNLMVVCQHVRNTFDKSVLDFKLTKPVGDINIK 104

[Close]

BLAST of Lsi02G013160 vs. ExPASy TrEMBL
Match: A0A6J1G901 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita moschata OX=3662 GN=LOC111452057 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.1e-45
Identity = 93/104 (89.42%), Postives = 97/104 (93.27%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDE+KSTFSMADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNTVGDINIK 104
           DPAREVLKDACQNL+ VCQHVRNT DKAV D KL   VGDINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

[Close]

BLAST of Lsi02G013160 vs. ExPASy TrEMBL
Match: A0A1S3C5G1 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo OX=3656 GN=LOC103497108 PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 7.0e-45
Identity = 95/108 (87.96%), Postives = 97/108 (89.81%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNT----VGDINIK 104
           DPAREVLKDACQNL+VVCQHVRNTFDKAV D  L        GDINI+
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKAKEEPEGDINIE 108

[Close]

BLAST of Lsi02G013160 vs. NCBI nr
Match: XP_038893936.1 (DNA-directed RNA polymerases I and III subunit RPAC2 [Benincasa hispida])

HSP 1 Score: 202.2 bits (513), Expect = 2.1e-48
Identity = 99/104 (95.19%), Postives = 100/104 (96.15%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNTVGDINIK 104
           DPAREVLKDACQNL+VVCQHVRNTFDKAV DIKL   VGDINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDIKLTKPVGDINIK 104

[Close]

BLAST of Lsi02G013160 vs. NCBI nr
Match: XP_004147276.2 (DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis sativus] >XP_011653666.1 DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis sativus])

HSP 1 Score: 198.0 bits (502), Expect = 4.0e-47
Identity = 96/104 (92.31%), Postives = 99/104 (95.19%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFS+ADEDHTLAN LRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNTVGDINIK 104
           DPAREVLKDACQNL+VVCQHVRNTFDKAV D+KL N V DINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDVKLTNPVRDINIK 104

[Close]

BLAST of Lsi02G013160 vs. NCBI nr
Match: KAE8652742.1 (hypothetical protein Csa_014245 [Cucumis sativus])

HSP 1 Score: 198.0 bits (502), Expect = 4.0e-47
Identity = 96/104 (92.31%), Postives = 99/104 (95.19%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFS+ADEDHTLAN LRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 97  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 156

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNTVGDINIK 104
           DPAREVLKDACQNL+VVCQHVRNTFDKAV D+KL N V DINIK
Sbjct: 157 DPAREVLKDACQNLMVVCQHVRNTFDKAVLDVKLTNPVRDINIK 200

[Close]

BLAST of Lsi02G013160 vs. NCBI nr
Match: XP_023522054.1 (DNA-directed RNA polymerases I and III subunit RPAC2-like [Cucurbita pepo subsp. pepo] >XP_023524948.1 DNA-directed RNA polymerases I and III subunit RPAC2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 193.7 bits (491), Expect = 7.6e-46
Identity = 94/104 (90.38%), Postives = 97/104 (93.27%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNTVGDINIK 104
           DPAREVLKDACQNL+ VCQHVRNT DKAV D KL   VGDINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

[Close]

BLAST of Lsi02G013160 vs. NCBI nr
Match: KAG7036964.1 (DNA-directed RNA polymerases I and III subunit RPAC2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 192.2 bits (487), Expect = 2.2e-45
Identity = 93/104 (89.42%), Postives = 97/104 (93.27%), Query Frame = 0


Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDE+KSTFSMADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 54  MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 113

Query: 61  DPAREVLKDACQNLIVVCQHVRNTFDKAV-DIKLNNTVGDINIK 104
           DPAREVLKDACQNL+ VCQHVRNT DKAV D KL   VGDINIK
Sbjct: 114 DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 157

[Close]

BLAST of Lsi02G013160 vs. TAIR 10
Match: AT2G29540.1 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 131.3 bits (329), Expect = 4.3e-31
Identity = 60/89 (67.42%), Postives = 76/89 (85.39%), Query Frame = 0


Query: 1  MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
          MEHGSFT+ + ++F++++EDHTLANA+R+ LNQDPRVT   Y+IPHPS  +VNIRVQTTG
Sbjct: 1  MEHGSFTNVSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTG 60

Query: 61 DPAREVLKDACQNLIVVCQHVRNTFDKAV 90
          DPAREV KDACQ L+ + +HVR+ FDKAV
Sbjct: 61 DPAREVFKDACQELMQMNRHVRSVFDKAV 89

[Close]

BLAST of Lsi02G013160 vs. TAIR 10
Match: AT2G29540.2 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 131.3 bits (329), Expect = 4.3e-31
Identity = 60/89 (67.42%), Postives = 76/89 (85.39%), Query Frame = 0


Query: 1  MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
          MEHGSFT+ + ++F++++EDHTLANA+R+ LNQDPRVT   Y+IPHPS  +VNIRVQTTG
Sbjct: 1  MEHGSFTNVSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTG 60

Query: 61 DPAREVLKDACQNLIVVCQHVRNTFDKAV 90
          DPAREV KDACQ L+ + +HVR+ FDKAV
Sbjct: 61 DPAREVFKDACQELMQMNRHVRSVFDKAV 89

[Close]

BLAST of Lsi02G013160 vs. TAIR 10
Match: AT2G29540.3 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 84.7 bits (208), Expect = 4.7e-17
Identity = 39/55 (70.91%), Postives = 45/55 (81.82%), Query Frame = 0


Query: 35  PRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLKDACQNLIVVCQHVRNTFDKAV 90
           PRVT   Y+IPHPS  +VNIRVQTTGDPAREV KDACQ L+ + +HVR+ FDKAV
Sbjct: 52  PRVTVAAYTIPHPSLEQVNIRVQTTGDPAREVFKDACQELMQMNRHVRSVFDKAV 106

[Close]

BLAST of Lsi02G013160 vs. TAIR 10
Match: AT3G52090.1 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 53.9 bits (128), Expect = 8.8e-08
Identity = 27/90 (30.00%), Postives = 49/90 (54.44%), Query Frame = 0


Query: 5   SFTDETK----STFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 64
           S+  +TK    ++F++  EDHT+ N +R  L++D  V F GY +PHP   ++ +R+ TT 
Sbjct: 20  SYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVRIHTTS 79

Query: 65  DPA-REVLKDACQNLIVVCQHVRNTFDKAV 90
             +  +    A  +L     +++N F+  V
Sbjct: 80  QSSPMQAYNQAINDLDKELDYLKNQFEAEV 109

[Close]

BLAST of Lsi02G013160 vs. TAIR 10
Match: AT3G52090.2 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 46.2 bits (108), Expect = 1.8e-05
Identity = 28/77 (36.36%), Postives = 43/77 (55.84%), Query Frame = 0


Query: 5  SFTDETK----STFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 64
          S+  +TK    ++F++  EDHT+ N +R  L++D  V F GY +PHP   ++ +R     
Sbjct: 20 SYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVR----- 79

Query: 65 DPAREVLKDACQNLIVV 78
          DP     KD  +NL VV
Sbjct: 80 DP-----KDWFRNLKVV 86

The following BLAST results are available for this feature:

BLAST of Lsi02G013160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XK67	5.9e-17	48.19	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Xenopus laevis OX=8355 G...	[more]
Q6DRI4	1.1e-15	50.00	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Danio rerio OX=7955 GN=p...	[more]
Q54ES6	1.1e-15	48.75	DNA-directed RNA polymerases I and III subunit rpc19 OS=Dictyostelium discoideum...	[more]
P28000	1.5e-15	45.78	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Saccharomyces cerevisiae...	[more]
P0DPB6	4.2e-15	42.22	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Homo sapiens OX=9606 GN=...	[more]

BLAST of Lsi02G013160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUT5	2.0e-47	92.31	RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G095010...	[more]
A0A6J1KDK3	1.1e-45	89.42	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita maxima OX=3661...	[more]
A0A6J1D9Q8	1.1e-45	89.42	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Momordica charantia OX=3...	[more]
A0A6J1G901	1.1e-45	89.42	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita moschata OX=36...	[more]
A0A1S3C5G1	7.0e-45	87.96	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo OX=3656 GN=...	[more]

BLAST of Lsi02G013160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893936.1	2.1e-48	95.19	DNA-directed RNA polymerases I and III subunit RPAC2 [Benincasa hispida]	[more]
XP_004147276.2	4.0e-47	92.31	DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis sativus] >XP_01165...	[more]
KAE8652742.1	4.0e-47	92.31	hypothetical protein Csa_014245 [Cucumis sativus]	[more]
XP_023522054.1	7.6e-46	90.38	DNA-directed RNA polymerases I and III subunit RPAC2-like [Cucurbita pepo subsp....	[more]
KAG7036964.1	2.2e-45	89.42	DNA-directed RNA polymerases I and III subunit RPAC2, partial [Cucurbita argyros...	[more]

BLAST of Lsi02G013160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29540.1	4.3e-31	67.42	RNApolymerase 14 kDa subunit	[more]
AT2G29540.2	4.3e-31	67.42	RNApolymerase 14 kDa subunit	[more]
AT2G29540.3	4.7e-17	70.91	RNApolymerase 14 kDa subunit	[more]
AT3G52090.1	8.8e-08	30.00	DNA-directed RNA polymerase, RBP11-like	[more]
AT3G52090.2	1.8e-05	36.36	DNA-directed RNA polymerase, RBP11-like	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036603	RNA polymerase, RBP11-like subunit	GENE3D	3.30.1360.10		coord: 2..95 e-value: 8.0E-35 score: 120.9
IPR036603	RNA polymerase, RBP11-like subunit	SUPERFAMILY	55257	RBP11-like subunits of RNA polymerase	coord: 8..90
IPR009025	DNA-directed RNA polymerase, RBP11-like dimerisation domain	PFAM	PF13656	RNA_pol_L_2	coord: 12..83 e-value: 1.3E-29 score: 101.7
None	No IPR available	PANTHER	PTHR13946	DNA-DIRECTED RNA POLYMERASE I,II,III	coord: 1..89
None	No IPR available	PANTHER	PTHR13946:SF37	SUBFAMILY NOT NAMED	coord: 1..89
IPR008193	DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site	PROSITE	PS01154	RNA_POL_L_13KD	coord: 16..47
IPR033898	DNA-directed RNA polymerases I and III subunit AC19	CDD	cd07029	RNAP_I_III_AC19	coord: 2..85 e-value: 9.23934E-43 score: 132.741

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G013160.1	Lsi02G013160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0046983	protein dimerization activity