Lsi02G012840 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G012840
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptioninositol 3-kinase
Locationchr02: 17177288 .. 17179799 (-)
RNA-Seq ExpressionLsi02G012840
SyntenyLsi02G012840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAGCGATTCCAAAGACCCACCTCCCAATTTCCCTCCTCGTGCCCTGATTGTTGGCAATTTCTGCCACGATTTCCTTATTCGCGATGCCCATGTCGTCACCGAGTCCCTCGGCGGCGCCGCCTCGTTTATTTCCACGGTCTTTGATGGCTTATCGGTTCCTTATCTCACTGTTTCGAAGGTAGGGGAAGATTTTGCCTACTCGACGAACCAGAGCCCGATTGTTGTGCCTGATTCGAAAACGACTACGTTTCGGGCGTTTTTCGATTCTTCAATCGCTGGTGAGGGGCATGGAGATCGGATTCTGAAAAGGGTTGTGGCCTGTGCTCCGATTTTGCCGTCTGATCTTCCTGATTTTAGATTTGATTTTGGAATGGCGGTTGGGGTTGGTGGGGAGGTTCTTCCAGAGACGCTTGAGCGGATGATTGAGATTTGTGATGATGTGTTTGTTGATGTTCAATCTTTGATTCGAGTGTTTGATGAAATTGATGGGACTGTTGAGCATGTGAATTTGAAGGAGAGTGGGTTCTTCCATTTGTTGCCTCGAATTGGGTTTCTGAAGGCCTCTGCAGAAGAGGCTCCGTTTATGGATATTGAAGAGGTACGGAAACTTTGCCCTGTTGTTGTGACGAACGGGAAAGAAGGATGTACCTTGTACTCGAACGGCAGCCAGTTGCAGATTGCTCCGTTCACAGCGACTCAACTCGATCCCACCGGCGCCGGTGACAGTTTCTTGGGTGGTTTTGCGGCAGGGTTCACGGCTGGATTAGCTGTGCCGGATGCTGCATTGCTTGGGAATTTGTTTGGATCTCTTACAGTCTCCCAAATTGGCTTGCCCCATTTCGAGTCCAGAATCTTGCAGGTTTGAACTCTGGATGAACAATTGATTATTATAGAAGATTGAAATGTTGGGTTCCTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTGGTAGTTTATTTGTTGCCTTTCTTCCCTTTGATGCTATCTTGAAGTAGTTGGACTCTGTAATTTCTTATTTAGAATCAACTCTAGGACAAGATGATCATATTATCGATTTTCTTTGTATCTGGTTGAATAAGATGAGATATCTGGTGAATTTTTCTGTGCTGAAGCTCTATAACAAAGTTTCAGCCGAAACGTGTCTTAGCTTAGTTCTATTCTGGTCTGGTCCAATAGGGAATGAACTTACAATCATGGGTTGGCGGGTTAAAGATTATAAGTTCATAAGCGTAGCTCATTCCCTTTTTTTAGCGGAATATATCTTCTAATTCTTAGAACTCTATCTCTTTAGTTTGTTTTCTTTAGAGCAAGTGAAAGTTTTCTTCTGAATTTTTGAATAGCCATTTCAAGTGCAAATAACTTTGCTTTTCACCCGTTGCCCATATCATAATTTTTGTAAAGGATTTTGAAAACAAAAGGAGGAAGCTATGTTTCCGAGCAGACTTTCGAAAACAAAAACCATGTTTGTCTGTGATTGTGTTGCTATATTTGTTAGCCATGAGAGAAAGACATCATCTTGTTTTGTTTAAGGTCTAGCGATTAGTCTACCGTTAGCCAAGTTTCTCAATAACAGTCGAATGTAAACAATGGAAATGGTCTGGTCAAAATTCCAGGTTTGTGGGGGCACTCGTTTACGTCAGCCCTTAGAAAGTCACTCCAAACATGCTGTTCATCTGATGTTGCTTTAAACAAACGCCTATAGATTATAGATTATGTTGTGAGAGACGACAGCAGATAGAGTACTGATGTTCTGATGTGTCCATAGTGAAACTCATTACTTCCTTTTGTAAAATTATGTTACTTTTAGTCTTTCTGGATGCATTTTTGTTTGGGGTTAGCTCGTGTTGCGTTTCCATAAGTACCGTTGAATCAACACTCACAGGTCCCTTCAAAATTGTTGATTTGATGAGATCTATCCTGTTTCTATTTTTCTCCCTTGTGAATGCTTGTATTGCTTGCTGTTGCTGCTGTGTGCTTTTTGAAGGAGATTTGGAAGTCCTTTAATTCCTTTTCCAGAGAAAAAAAAGTGCTTCTTTTGGAAGTTGAAAGCAGGAACTGACATCTAATACTGAGTGTCGATTCTATTATAGTGAACTCATTACTTCCCTTTGTGCTGCAGACAATTAAGGACGAGGTAGAGAGAAGAAAGCTGCAGTGCTTGGACTCGTGCGACTCGGGAGAGAACAAGTTGATCCATCAGATGCCAGAAGGACACGAACAGTTCCAAAAATTGCTTGGGACGGTCAGATCGGAATGCCAATTGAGTCTGCCAGCTTCTCCCAGAGCAGTGGAGCCAGTGAATGGGCAACATAAGTTATTATGA

mRNA sequence

ATGGTGAGCGATTCCAAAGACCCACCTCCCAATTTCCCTCCTCGTGCCCTGATTGTTGGCAATTTCTGCCACGATTTCCTTATTCGCGATGCCCATGTCGTCACCGAGTCCCTCGGCGGCGCCGCCTCGTTTATTTCCACGGTCTTTGATGGCTTATCGGTTCCTTATCTCACTGTTTCGAAGGTAGGGGAAGATTTTGCCTACTCGACGAACCAGAGCCCGATTGTTGTGCCTGATTCGAAAACGACTACGTTTCGGGCGTTTTTCGATTCTTCAATCGCTGGTGAGGGGCATGGAGATCGGATTCTGAAAAGGGTTGTGGCCTGTGCTCCGATTTTGCCGTCTGATCTTCCTGATTTTAGATTTGATTTTGGAATGGCGGTTGGGGTTGGTGGGGAGGTTCTTCCAGAGACGCTTGAGCGGATGATTGAGATTTGTGATGATGTGTTTGTTGATGTTCAATCTTTGATTCGAGTGTTTGATGAAATTGATGGGACTGTTGAGCATGTGAATTTGAAGGAGAGTGGGTTCTTCCATTTGTTGCCTCGAATTGGGTTTCTGAAGGCCTCTGCAGAAGAGGCTCCGTTTATGGATATTGAAGAGGTACGGAAACTTTGCCCTGTTGTTGTGACGAACGGGAAAGAAGGATGTACCTTGTACTCGAACGGCAGCCAGTTGCAGATTGCTCCGTTCACAGCGACTCAACTCGATCCCACCGGCGCCGGTGACAGTTTCTTGGGTGGTTTTGCGGCAGGGTTCACGGCTGGATTAGCTGTGCCGGATGCTGCATTGCTTGGGAATTTGTTTGGATCTCTTACAGTCTCCCAAATTGGCTTGCCCCATTTCGAGTCCAGAATCTTGCAGACAATTAAGGACGAGGTAGAGAGAAGAAAGCTGCAGTGCTTGGACTCGTGCGACTCGGGAGAGAACAAGTTGATCCATCAGATGCCAGAAGGACACGAACAGTTCCAAAAATTGCTTGGGACGGTCAGATCGGAATGCCAATTGAGTCTGCCAGCTTCTCCCAGAGCAGTGGAGCCAGTGAATGGGCAACATAAGTTATTATGA

Coding sequence (CDS)

ATGGTGAGCGATTCCAAAGACCCACCTCCCAATTTCCCTCCTCGTGCCCTGATTGTTGGCAATTTCTGCCACGATTTCCTTATTCGCGATGCCCATGTCGTCACCGAGTCCCTCGGCGGCGCCGCCTCGTTTATTTCCACGGTCTTTGATGGCTTATCGGTTCCTTATCTCACTGTTTCGAAGGTAGGGGAAGATTTTGCCTACTCGACGAACCAGAGCCCGATTGTTGTGCCTGATTCGAAAACGACTACGTTTCGGGCGTTTTTCGATTCTTCAATCGCTGGTGAGGGGCATGGAGATCGGATTCTGAAAAGGGTTGTGGCCTGTGCTCCGATTTTGCCGTCTGATCTTCCTGATTTTAGATTTGATTTTGGAATGGCGGTTGGGGTTGGTGGGGAGGTTCTTCCAGAGACGCTTGAGCGGATGATTGAGATTTGTGATGATGTGTTTGTTGATGTTCAATCTTTGATTCGAGTGTTTGATGAAATTGATGGGACTGTTGAGCATGTGAATTTGAAGGAGAGTGGGTTCTTCCATTTGTTGCCTCGAATTGGGTTTCTGAAGGCCTCTGCAGAAGAGGCTCCGTTTATGGATATTGAAGAGGTACGGAAACTTTGCCCTGTTGTTGTGACGAACGGGAAAGAAGGATGTACCTTGTACTCGAACGGCAGCCAGTTGCAGATTGCTCCGTTCACAGCGACTCAACTCGATCCCACCGGCGCCGGTGACAGTTTCTTGGGTGGTTTTGCGGCAGGGTTCACGGCTGGATTAGCTGTGCCGGATGCTGCATTGCTTGGGAATTTGTTTGGATCTCTTACAGTCTCCCAAATTGGCTTGCCCCATTTCGAGTCCAGAATCTTGCAGACAATTAAGGACGAGGTAGAGAGAAGAAAGCTGCAGTGCTTGGACTCGTGCGACTCGGGAGAGAACAAGTTGATCCATCAGATGCCAGAAGGACACGAACAGTTCCAAAAATTGCTTGGGACGGTCAGATCGGAATGCCAATTGAGTCTGCCAGCTTCTCCCAGAGCAGTGGAGCCAGTGAATGGGCAACATAAGTTATTATGA

Protein sequence

MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVSKVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDFRFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHLLPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTGAGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQCLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNGQHKLL
Homology
BLAST of Lsi02G012840 vs. ExPASy Swiss-Prot
Match: Q93Z01 (Inositol 3-kinase OS=Arabidopsis thaliana OX=3702 GN=At5g58730 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 6.6e-108
Identity = 193/320 (60.31%), Postives = 233/320 (72.81%), Query Frame = 0

Query: 15  RALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVSKVGEDFAYSTNQSP 74
           R LIVGN+CHD LI++  VV E+LGGAASFIS V D  SV    VSKVG DF Y    SP
Sbjct: 22  RVLIVGNYCHDVLIQNGSVVAETLGGAASFISNVLDSSSVSCDLVSKVGHDFRYEVTHSP 81

Query: 75  IVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDFRFDFGMAVGVGGEV 134
           IV P+ +TT F A+FD  I G GH DR+LKRV AC PILPSD+PD RFDFGMAVGVGGE+
Sbjct: 82  IVAPEKETTVFEAYFDLGIDGIGHADRVLKRVSACDPILPSDIPDSRFDFGMAVGVGGEI 141

Query: 135 LPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHLLPRIGFLKASAEEA 194
           LPETLE+M+EICD V VD+Q+LIRVFD +DG V+ V+LKESGF+H+L RIGFLKAS++EA
Sbjct: 142 LPETLEKMVEICDVVAVDIQALIRVFDPVDGAVKLVDLKESGFYHILHRIGFLKASSDEA 201

Query: 195 PFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTGAGDSFLGGFAAGFT 254
            FMD+E+++ LC VVVTNG++GC +Y    ++ + PF A Q+DPTGAGDSFLGG   G  
Sbjct: 202 LFMDVEQMKHLCCVVVTNGEKGCRIYHKDDEMTVPPFLAKQVDPTGAGDSFLGGLIVGLV 261

Query: 255 AGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQCLDSCDSGENKLIH 314
            GL VPDAALLGNLFGS+TV  IG P F+  +LQ +KDEV+RRK QC  +  S  N    
Sbjct: 262 EGLTVPDAALLGNLFGSITVEHIGQPKFDLMMLQKVKDEVQRRKKQC--NISSSHN---- 321

Query: 315 QMPEGHEQFQKLLGTVRSEC 335
                H +F + L   R  C
Sbjct: 322 ---NDHNEFHERLSPARFSC 332

BLAST of Lsi02G012840 vs. ExPASy Swiss-Prot
Match: Q5GA22 (Inositol 3-kinase OS=Zea mays OX=4577 GN=MIK PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.6e-80
Identity = 159/340 (46.76%), Postives = 219/340 (64.41%), Query Frame = 0

Query: 17  LIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLS---------VPYLTVSKVGEDFA 76
           L+VG++CHD LIR   +V E+LGGAA+F+S V D  S          P++ V+KVG DF 
Sbjct: 32  LVVGSYCHDVLIRGGRIVGETLGGAAAFVSNVLDAASPQGAALNETSPFVVVAKVGHDFI 91

Query: 77  Y----STNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDFRFD 136
           Y    ++ + P ++  S TT+F A F  + A     DR L+RV AC PI P+DLPD RF 
Sbjct: 92  YARAPASARHPPLLCSSPTTSFHAQFSETAASAHAPDRELRRVRACDPIYPADLPDRRFA 151

Query: 137 FGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHLLPR 196
           +G+AVGV GEVLPETLE+MI +C  V VD Q+LIR FD  DG V HV L ++ +  LLPR
Sbjct: 152 YGLAVGVAGEVLPETLEQMIRLCRTVLVDAQALIRAFDG-DGAVGHVALGDTPYARLLPR 211

Query: 197 IGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTGAGD 256
           + F+KAS+EEAP++ +E  R+ C V+VT G++GC LY +G +  +APF A Q+DPTGAGD
Sbjct: 212 VAFVKASSEEAPYVGVETTRRQCCVIVTEGRDGCRLYWDGGEAHVAPFPAVQVDPTGAGD 271

Query: 257 SFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQCLD 316
           SFL GFAAG   GL+  DAALLGN FG+  VSQ+G+P F  ++LQ +K+ +E +  +   
Sbjct: 272 SFLAGFAAGLLWGLSATDAALLGNFFGAAAVSQVGVPTFHPKMLQAVKEILEEKTRKRSS 331

Query: 317 SCDSGENKLIHQMPEGHEQFQKL--LGTVRSECQLSLPAS 342
            C +G +  + +    +E    L     + SE Q + PA+
Sbjct: 332 PCMNGASFTLEKSNMHNELHAALQEAAVLMSEQQQADPAN 370

BLAST of Lsi02G012840 vs. ExPASy Swiss-Prot
Match: Q84R36 (Inositol 3-kinase OS=Oryza sativa subsp. japonica OX=39947 GN=MIK PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-79
Identity = 153/290 (52.76%), Postives = 199/290 (68.62%), Query Frame = 0

Query: 17  LIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLS---VPYLTVSKVGEDFAYSTNQS 76
           L+VG++CHD L+R   VV E+LGGAA+F+S V D  S        VSKVG DFAY+T  +
Sbjct: 45  LVVGSYCHDVLLRGGRVVGETLGGAAAFVSNVLDAASPAGASLAVVSKVGHDFAYATAAA 104

Query: 77  PI----VVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDFRFDFGMAVG 136
           P     V+  S TT+F A F    A     DR L+RV AC PI P+DLPD RF +G+AVG
Sbjct: 105 PARHPPVLCASPTTSFHARFSDDAASAHAPDRQLRRVHACDPIYPADLPDRRFAYGLAVG 164

Query: 137 VGGEVLPETLERMIEICDDVFVDVQSLIRVFD---EIDGTVEHVNLKESGFFHLLPRIGF 196
           V GEVLPETLERMI +C  V VD Q+LIR FD   +  G V HV L+ + +  LLPR+ F
Sbjct: 165 VAGEVLPETLERMIRLCRAVLVDAQALIRAFDGEAKGGGAVRHVALEATPYARLLPRVAF 224

Query: 197 LKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTGAGDSFL 256
           LKAS+EEAP++ +E  R+ C V+VT G++GC LY +G + ++APF A Q+DPTGAGDSFL
Sbjct: 225 LKASSEEAPYVGVETARRRCCVIVTEGRDGCRLYWDGGEARVAPFPAVQVDPTGAGDSFL 284

Query: 257 GGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVER 297
            GFA+G   GL+  DAALLGN FG+  VSQ+G+P F+ ++LQ +K  +E+
Sbjct: 285 AGFASGLLWGLSATDAALLGNFFGAAAVSQVGVPTFDPKMLQAVKQILEK 334

BLAST of Lsi02G012840 vs. ExPASy Swiss-Prot
Match: Q83JB1 (Fructoselysine 6-kinase OS=Shigella flexneri OX=623 GN=frlD PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 5.6e-06
Identity = 34/104 (32.69%), Postives = 53/104 (50.96%), Query Frame = 0

Query: 180 LLPRIGFLKASA---EEAPFMDIEEV--RKLCPVVVTNGKEGCTLYSNGSQLQIAPFTAT 239
           L+P + F  ASA   +EA  + ++ +  R    V+VT G+ G   +      + AP   T
Sbjct: 156 LVPHLDFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVT 215

Query: 240 QLDPTGAGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIG 279
            +D  GAGDSF+ GF  G++AG+ +P A   G    + T+   G
Sbjct: 216 VIDTMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHG 259

BLAST of Lsi02G012840 vs. ExPASy Swiss-Prot
Match: Q53W83 (2-dehydro-3-deoxygluconokinase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=kdgK PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 5.6e-06
Identity = 34/103 (33.01%), Postives = 51/103 (49.51%), Query Frame = 0

Query: 181 LPRIGFLKASAEEAP--FMDIEEVRKLC---PVVVTNGKEGCTLYSNGSQLQIAPFTATQ 240
           LP +  L  S EEA   F  +EE  +      VV+  G +G   + +G +++ + F    
Sbjct: 184 LPGVDLLFLSEEEAELLFGRVEEALRALSAPEVVLKRGAKGAWAFVDGRRVEGSAFAVEA 243

Query: 241 LDPTGAGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIG 279
           +DP GAGD+F  G+ AG   GL V +   L NL G+   +  G
Sbjct: 244 VDPVGAGDAFAAGYLAGAVWGLPVEERLRLANLLGASVAASRG 286

BLAST of Lsi02G012840 vs. ExPASy TrEMBL
Match: A0A5A7SRA5 (Inositol 3-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001210 PE=4 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 6.9e-185
Identity = 323/352 (91.76%), Postives = 336/352 (95.45%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MVSDSKD PPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS
Sbjct: 1   MVSDSKDLPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVGEDFAYSTNQSPIVV +SKTT FRAFFDSSI+G+G  DRILKRV AC+PILPSDLPDF
Sbjct: 61  KVGEDFAYSTNQSPIVVSNSKTTAFRAFFDSSISGDGRRDRILKRVAACSPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RFDFGMAVGVGGEV+PETLERMIEICD VFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL
Sbjct: 121 RFDFGMAVGVGGEVVPETLERMIEICDAVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           L RIGFLKASAEEAPFMD+EEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTA Q+DPTG
Sbjct: 181 LSRIGFLKASAEEAPFMDVEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTANQVDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQ+GLPHFESRILQ IKDEVERRK+Q
Sbjct: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQVGLPHFESRILQRIKDEVERRKVQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNGQH 353
           C+D CD GE+K IH+MPEGHEQFQKLLGTVRSECQLSLPASP AVE VNG +
Sbjct: 301 CMDLCDPGESKSIHRMPEGHEQFQKLLGTVRSECQLSLPASPIAVEQVNGHY 352

BLAST of Lsi02G012840 vs. ExPASy TrEMBL
Match: A0A1S3C5B8 (inositol 3-kinase OS=Cucumis melo OX=3656 GN=LOC103497081 PE=4 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 6.9e-185
Identity = 323/352 (91.76%), Postives = 336/352 (95.45%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MVSDSKD PPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS
Sbjct: 1   MVSDSKDLPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVGEDFAYSTNQSPIVV +SKTT FRAFFDSSI+G+G  DRILKRV AC+PILPSDLPDF
Sbjct: 61  KVGEDFAYSTNQSPIVVSNSKTTAFRAFFDSSISGDGRRDRILKRVAACSPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RFDFGMAVGVGGEV+PETLERMIEICD VFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL
Sbjct: 121 RFDFGMAVGVGGEVVPETLERMIEICDAVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           L RIGFLKASAEEAPFMD+EEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTA Q+DPTG
Sbjct: 181 LSRIGFLKASAEEAPFMDVEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTANQVDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQ+GLPHFESRILQ IKDEVERRK+Q
Sbjct: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQVGLPHFESRILQRIKDEVERRKVQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNGQH 353
           C+D CD GE+K IH+MPEGHEQFQKLLGTVRSECQLSLPASP AVE VNG +
Sbjct: 301 CMDLCDPGESKSIHRMPEGHEQFQKLLGTVRSECQLSLPASPIAVEQVNGHY 352

BLAST of Lsi02G012840 vs. ExPASy TrEMBL
Match: A0A0A0M0S7 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530670 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.0e-183
Identity = 320/352 (90.91%), Postives = 333/352 (94.60%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPY+TVS
Sbjct: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYVTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVGEDFAYSTNQSPIVV +SKTT FRAFFDSSI+G+G  DRILKRV AC+PILPSDLPDF
Sbjct: 61  KVGEDFAYSTNQSPIVVSNSKTTAFRAFFDSSISGDGRRDRILKRVAACSPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RFDFGMAVGVGGEV+PETLERMIEICD VFVDVQSLIRVFDEIDGTVEHV+LKESGFFHL
Sbjct: 121 RFDFGMAVGVGGEVVPETLERMIEICDAVFVDVQSLIRVFDEIDGTVEHVDLKESGFFHL 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           LPRIGFLKASAEEAPFMD+EE RKLCPVVVTNGKEGCTLYSNGSQLQIAPF  TQ+DPTG
Sbjct: 181 LPRIGFLKASAEEAPFMDVEEARKLCPVVVTNGKEGCTLYSNGSQLQIAPFPVTQVDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGDSFLGGFAAGF AGLAV DAALLGNLFGSLTVSQIGLPHFESRI+Q IKDEVERRK+Q
Sbjct: 241 AGDSFLGGFAAGFAAGLAVADAALLGNLFGSLTVSQIGLPHFESRIVQRIKDEVERRKVQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNGQH 353
           C+DSC  GENK IHQMPEGHEQFQKLLGTVRSECQLSLP SP AVE VNG +
Sbjct: 301 CMDSCHPGENKSIHQMPEGHEQFQKLLGTVRSECQLSLPTSPIAVEQVNGHY 352

BLAST of Lsi02G012840 vs. ExPASy TrEMBL
Match: A0A6J1I3U1 (inositol 3-kinase-like OS=Cucurbita maxima OX=3661 GN=LOC111469415 PE=4 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 4.7e-181
Identity = 315/355 (88.73%), Postives = 332/355 (93.52%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MV+DSKDP PNFPPRALIVGNFCHDFLIRDA VVTESLGGAASFISTVFDGLSVPYLTVS
Sbjct: 1   MVTDSKDPLPNFPPRALIVGNFCHDFLIRDARVVTESLGGAASFISTVFDGLSVPYLTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVG DFAYSTNQ+PIVV DSKTTTFRAFF SSIAG+GHGDRILKR+ ACAPILPSDLPDF
Sbjct: 61  KVGADFAYSTNQTPIVVADSKTTTFRAFFGSSIAGDGHGDRILKRIAACAPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RFDFGMAVGVGGE+LPETL RM+EICD VFVD+QSLIRVFDEIDGTVEHVNLKESGFFHL
Sbjct: 121 RFDFGMAVGVGGEILPETLTRMLEICDAVFVDIQSLIRVFDEIDGTVEHVNLKESGFFHL 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           LPRIGFLKASAEEAPFMD+EEVRK CPV+VTNGKEGCT+YSNG QLQIAPFTA +LDPTG
Sbjct: 181 LPRIGFLKASAEEAPFMDVEEVRKFCPVLVTNGKEGCTVYSNGGQLQIAPFTAAELDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGD FLGGFAAGFTAGLAV DAALLGNLFGSLTVSQ+GLP FESRILQ IKD+VE RKLQ
Sbjct: 241 AGDCFLGGFAAGFTAGLAVSDAALLGNLFGSLTVSQMGLPQFESRILQRIKDKVEMRKLQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNGQHKLL 356
            +DSCD GE K IHQMPEGHEQF+KLL TVRSECQL++PASPR VE VNGQHKLL
Sbjct: 301 HIDSCDPGERKSIHQMPEGHEQFKKLLVTVRSECQLNVPASPRTVEQVNGQHKLL 355

BLAST of Lsi02G012840 vs. ExPASy TrEMBL
Match: A0A6J1KD46 (inositol 3-kinase-like OS=Cucurbita maxima OX=3661 GN=LOC111492784 PE=4 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 1.5e-179
Identity = 317/350 (90.57%), Postives = 331/350 (94.57%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MVSD+KDPPPNFPPRALIVGNFCHD LIRDAHVVTESLGGAASFISTVF+GLSVPYLTVS
Sbjct: 1   MVSDTKDPPPNFPPRALIVGNFCHDILIRDAHVVTESLGGAASFISTVFEGLSVPYLTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVGEDFAYSTNQSPIVV +SKTTTFRAFFDSSIAG+GHGDRILKRV ACAPILPSDLPDF
Sbjct: 61  KVGEDFAYSTNQSPIVVGNSKTTTFRAFFDSSIAGDGHGDRILKRVSACAPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RF+FGMAVGVGGEVLPETLERMIEICD VFVD+QSLIRVFDEIDGTV+HVNLKESGFFHL
Sbjct: 121 RFEFGMAVGVGGEVLPETLERMIEICDVVFVDIQSLIRVFDEIDGTVQHVNLKESGFFHL 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           LPRIG LKASAEEAPFMD EEV+KLCPVVVTNGKEGC LYSNGS LQIAPFTATQLDPTG
Sbjct: 181 LPRIGLLKASAEEAPFMDAEEVKKLCPVVVTNGKEGCKLYSNGSVLQIAPFTATQLDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTV+QIGLPHFESRILQ IKDEV+RRKLQ
Sbjct: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVAQIGLPHFESRILQRIKDEVDRRKLQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNG 351
            +DS   GEN+LIH +PEGHEQF K LGTVRSE QL+LPAS RAVE VNG
Sbjct: 301 FIDSLSPGENELIHWIPEGHEQFHKSLGTVRSEFQLNLPASHRAVEQVNG 350

BLAST of Lsi02G012840 vs. NCBI nr
Match: XP_038894387.1 (inositol 3-kinase [Benincasa hispida])

HSP 1 Score: 672.5 bits (1734), Expect = 1.9e-189
Identity = 335/356 (94.10%), Postives = 344/356 (96.63%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MV+DSKDPPPNFP RALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS
Sbjct: 1   MVTDSKDPPPNFPHRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVGEDFAYST QSPIVV +SKTTTFRAFFDSSIAG+GH DRILKRVVACAPILPSDLPDF
Sbjct: 61  KVGEDFAYSTKQSPIVVANSKTTTFRAFFDSSIAGDGHRDRILKRVVACAPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RFDFGMAVGVGGEVLPETLERMIEICD VFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL
Sbjct: 121 RFDFGMAVGVGGEVLPETLERMIEICDAVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           LPRIGFLKASAEEAPFMD++EVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG
Sbjct: 181 LPRIGFLKASAEEAPFMDVDEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQ IKDEVERRKLQ
Sbjct: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQRIKDEVERRKLQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPA-SPRAVEPVNGQHKLL 356
            +DSCD GENK IH+MPEGHEQFQKLLGTVRSECQL+LPA SP A+E VNGQHKLL
Sbjct: 301 LMDSCDPGENKSIHRMPEGHEQFQKLLGTVRSECQLNLPASSPIAMEQVNGQHKLL 356

BLAST of Lsi02G012840 vs. NCBI nr
Match: XP_008457373.1 (PREDICTED: inositol 3-kinase [Cucumis melo] >KAA0031819.1 inositol 3-kinase [Cucumis melo var. makuwa] >TYJ97315.1 inositol 3-kinase [Cucumis melo var. makuwa])

HSP 1 Score: 656.4 bits (1692), Expect = 1.4e-184
Identity = 323/352 (91.76%), Postives = 336/352 (95.45%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MVSDSKD PPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS
Sbjct: 1   MVSDSKDLPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVGEDFAYSTNQSPIVV +SKTT FRAFFDSSI+G+G  DRILKRV AC+PILPSDLPDF
Sbjct: 61  KVGEDFAYSTNQSPIVVSNSKTTAFRAFFDSSISGDGRRDRILKRVAACSPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RFDFGMAVGVGGEV+PETLERMIEICD VFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL
Sbjct: 121 RFDFGMAVGVGGEVVPETLERMIEICDAVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           L RIGFLKASAEEAPFMD+EEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTA Q+DPTG
Sbjct: 181 LSRIGFLKASAEEAPFMDVEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTANQVDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQ+GLPHFESRILQ IKDEVERRK+Q
Sbjct: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQVGLPHFESRILQRIKDEVERRKVQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNGQH 353
           C+D CD GE+K IH+MPEGHEQFQKLLGTVRSECQLSLPASP AVE VNG +
Sbjct: 301 CMDLCDPGESKSIHRMPEGHEQFQKLLGTVRSECQLSLPASPIAVEQVNGHY 352

BLAST of Lsi02G012840 vs. NCBI nr
Match: XP_004145256.2 (inositol 3-kinase [Cucumis sativus])

HSP 1 Score: 652.5 bits (1682), Expect = 2.1e-183
Identity = 320/352 (90.91%), Postives = 332/352 (94.32%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPY+TVS
Sbjct: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYVTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVGEDFAYSTNQSPIVV DS TT FRAFFDSSI+G+G  DRILKRV AC+PILPSDLPDF
Sbjct: 61  KVGEDFAYSTNQSPIVVSDSNTTAFRAFFDSSISGDGRRDRILKRVAACSPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RFDFGMAVGVGGEV+PETLERMIEICD VFVDVQSLIRVFDEIDGTVEHV+LKESGFFHL
Sbjct: 121 RFDFGMAVGVGGEVVPETLERMIEICDAVFVDVQSLIRVFDEIDGTVEHVDLKESGFFHL 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           LPRIGFLKASAEEAPFMD+EE RKLCPVVVTNGKEGCTLYSNGSQLQIAPF  TQ+DPTG
Sbjct: 181 LPRIGFLKASAEEAPFMDVEEARKLCPVVVTNGKEGCTLYSNGSQLQIAPFPVTQVDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGDSFLGGFAAGF AGLAV DAALLGNLFGSLTVSQIGLPHFESRI+Q IKDEVERRK+Q
Sbjct: 241 AGDSFLGGFAAGFAAGLAVADAALLGNLFGSLTVSQIGLPHFESRIVQRIKDEVERRKVQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNGQH 353
           C+DSC  GENK IHQMPEGHEQFQKLLGTVRSECQLSLP SP AVE VNG +
Sbjct: 301 CMDSCHPGENKSIHQMPEGHEQFQKLLGTVRSECQLSLPTSPIAVEQVNGHY 352

BLAST of Lsi02G012840 vs. NCBI nr
Match: XP_022970433.1 (inositol 3-kinase-like [Cucurbita maxima])

HSP 1 Score: 643.7 bits (1659), Expect = 9.6e-181
Identity = 315/355 (88.73%), Postives = 332/355 (93.52%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MV+DSKDP PNFPPRALIVGNFCHDFLIRDA VVTESLGGAASFISTVFDGLSVPYLTVS
Sbjct: 1   MVTDSKDPLPNFPPRALIVGNFCHDFLIRDARVVTESLGGAASFISTVFDGLSVPYLTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVG DFAYSTNQ+PIVV DSKTTTFRAFF SSIAG+GHGDRILKR+ ACAPILPSDLPDF
Sbjct: 61  KVGADFAYSTNQTPIVVADSKTTTFRAFFGSSIAGDGHGDRILKRIAACAPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RFDFGMAVGVGGE+LPETL RM+EICD VFVD+QSLIRVFDEIDGTVEHVNLKESGFFHL
Sbjct: 121 RFDFGMAVGVGGEILPETLTRMLEICDAVFVDIQSLIRVFDEIDGTVEHVNLKESGFFHL 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           LPRIGFLKASAEEAPFMD+EEVRK CPV+VTNGKEGCT+YSNG QLQIAPFTA +LDPTG
Sbjct: 181 LPRIGFLKASAEEAPFMDVEEVRKFCPVLVTNGKEGCTVYSNGGQLQIAPFTAAELDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGD FLGGFAAGFTAGLAV DAALLGNLFGSLTVSQ+GLP FESRILQ IKD+VE RKLQ
Sbjct: 241 AGDCFLGGFAAGFTAGLAVSDAALLGNLFGSLTVSQMGLPQFESRILQRIKDKVEMRKLQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNGQHKLL 356
            +DSCD GE K IHQMPEGHEQF+KLL TVRSECQL++PASPR VE VNGQHKLL
Sbjct: 301 HIDSCDPGERKSIHQMPEGHEQFKKLLVTVRSECQLNVPASPRTVEQVNGQHKLL 355

BLAST of Lsi02G012840 vs. NCBI nr
Match: XP_023523268.1 (inositol 3-kinase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023523269.1 inositol 3-kinase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 640.2 bits (1650), Expect = 1.1e-179
Identity = 315/350 (90.00%), Postives = 331/350 (94.57%), Query Frame = 0

Query: 1   MVSDSKDPPPNFPPRALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVS 60
           MVSD+KDPPPNFPPRALIVGNFCHD LIRDAHVVTESLGGAASFISTVF+GLSV YLTVS
Sbjct: 1   MVSDTKDPPPNFPPRALIVGNFCHDILIRDAHVVTESLGGAASFISTVFEGLSVTYLTVS 60

Query: 61  KVGEDFAYSTNQSPIVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDF 120
           KVGEDFAYSTNQSPIVV +SKTTTFRAFFDSSIAG+GHGDRILKRV ACAPILPSDLPDF
Sbjct: 61  KVGEDFAYSTNQSPIVVGNSKTTTFRAFFDSSIAGDGHGDRILKRVSACAPILPSDLPDF 120

Query: 121 RFDFGMAVGVGGEVLPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHL 180
           RF+FGMAVGVGGEVLPETLERMIEICD VFVD+QSLIRVFDEIDGTV+HVNLKESGFFH+
Sbjct: 121 RFEFGMAVGVGGEVLPETLERMIEICDVVFVDIQSLIRVFDEIDGTVQHVNLKESGFFHM 180

Query: 181 LPRIGFLKASAEEAPFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTG 240
           LPRIG LKASAEEAPFMD+EEV+KLCPVVVTNGKEGC LYSNG  LQIAPFTATQLDPTG
Sbjct: 181 LPRIGLLKASAEEAPFMDVEEVKKLCPVVVTNGKEGCKLYSNGGVLQIAPFTATQLDPTG 240

Query: 241 AGDSFLGGFAAGFTAGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQ 300
           AGDSFLGGFAAGFTAGL VPDAALLGNLFGSLTV+QIGLPHFESRILQ IKDEV+RRKLQ
Sbjct: 241 AGDSFLGGFAAGFTAGLTVPDAALLGNLFGSLTVAQIGLPHFESRILQRIKDEVDRRKLQ 300

Query: 301 CLDSCDSGENKLIHQMPEGHEQFQKLLGTVRSECQLSLPASPRAVEPVNG 351
            +DS   GEN+LIH+MPEGHEQF K LGTVRSECQL+LPAS RAVE VNG
Sbjct: 301 FIDSLSPGENELIHRMPEGHEQFHKSLGTVRSECQLNLPASHRAVEQVNG 350

BLAST of Lsi02G012840 vs. TAIR 10
Match: AT5G58730.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 392.1 bits (1006), Expect = 4.7e-109
Identity = 193/320 (60.31%), Postives = 233/320 (72.81%), Query Frame = 0

Query: 15  RALIVGNFCHDFLIRDAHVVTESLGGAASFISTVFDGLSVPYLTVSKVGEDFAYSTNQSP 74
           R LIVGN+CHD LI++  VV E+LGGAASFIS V D  SV    VSKVG DF Y    SP
Sbjct: 22  RVLIVGNYCHDVLIQNGSVVAETLGGAASFISNVLDSSSVSCDLVSKVGHDFRYEVTHSP 81

Query: 75  IVVPDSKTTTFRAFFDSSIAGEGHGDRILKRVVACAPILPSDLPDFRFDFGMAVGVGGEV 134
           IV P+ +TT F A+FD  I G GH DR+LKRV AC PILPSD+PD RFDFGMAVGVGGE+
Sbjct: 82  IVAPEKETTVFEAYFDLGIDGIGHADRVLKRVSACDPILPSDIPDSRFDFGMAVGVGGEI 141

Query: 135 LPETLERMIEICDDVFVDVQSLIRVFDEIDGTVEHVNLKESGFFHLLPRIGFLKASAEEA 194
           LPETLE+M+EICD V VD+Q+LIRVFD +DG V+ V+LKESGF+H+L RIGFLKAS++EA
Sbjct: 142 LPETLEKMVEICDVVAVDIQALIRVFDPVDGAVKLVDLKESGFYHILHRIGFLKASSDEA 201

Query: 195 PFMDIEEVRKLCPVVVTNGKEGCTLYSNGSQLQIAPFTATQLDPTGAGDSFLGGFAAGFT 254
            FMD+E+++ LC VVVTNG++GC +Y    ++ + PF A Q+DPTGAGDSFLGG   G  
Sbjct: 202 LFMDVEQMKHLCCVVVTNGEKGCRIYHKDDEMTVPPFLAKQVDPTGAGDSFLGGLIVGLV 261

Query: 255 AGLAVPDAALLGNLFGSLTVSQIGLPHFESRILQTIKDEVERRKLQCLDSCDSGENKLIH 314
            GL VPDAALLGNLFGS+TV  IG P F+  +LQ +KDEV+RRK QC  +  S  N    
Sbjct: 262 EGLTVPDAALLGNLFGSITVEHIGQPKFDLMMLQKVKDEVQRRKKQC--NISSSHN---- 321

Query: 315 QMPEGHEQFQKLLGTVRSEC 335
                H +F + L   R  C
Sbjct: 322 ---NDHNEFHERLSPARFSC 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z016.6e-10860.31Inositol 3-kinase OS=Arabidopsis thaliana OX=3702 GN=At5g58730 PE=2 SV=1[more]
Q5GA222.6e-8046.76Inositol 3-kinase OS=Zea mays OX=4577 GN=MIK PE=1 SV=1[more]
Q84R361.3e-7952.76Inositol 3-kinase OS=Oryza sativa subsp. japonica OX=39947 GN=MIK PE=2 SV=1[more]
Q83JB15.6e-0632.69Fructoselysine 6-kinase OS=Shigella flexneri OX=623 GN=frlD PE=3 SV=1[more]
Q53W835.6e-0633.012-dehydro-3-deoxygluconokinase OS=Thermus thermophilus (strain ATCC 27634 / DSM ... [more]
Match NameE-valueIdentityDescription
A0A5A7SRA56.9e-18591.76Inositol 3-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00... [more]
A0A1S3C5B86.9e-18591.76inositol 3-kinase OS=Cucumis melo OX=3656 GN=LOC103497081 PE=4 SV=1[more]
A0A0A0M0S71.0e-18390.91PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530670 PE=4 S... [more]
A0A6J1I3U14.7e-18188.73inositol 3-kinase-like OS=Cucurbita maxima OX=3661 GN=LOC111469415 PE=4 SV=1[more]
A0A6J1KD461.5e-17990.57inositol 3-kinase-like OS=Cucurbita maxima OX=3661 GN=LOC111492784 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038894387.11.9e-18994.10inositol 3-kinase [Benincasa hispida][more]
XP_008457373.11.4e-18491.76PREDICTED: inositol 3-kinase [Cucumis melo] >KAA0031819.1 inositol 3-kinase [Cuc... [more]
XP_004145256.22.1e-18390.91inositol 3-kinase [Cucumis sativus][more]
XP_022970433.19.6e-18188.73inositol 3-kinase-like [Cucurbita maxima][more]
XP_023523268.11.1e-17990.00inositol 3-kinase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023523269.1 i... [more]
Match NameE-valueIdentityDescription
AT5G58730.14.7e-10960.31pfkB-like carbohydrate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011611Carbohydrate kinase PfkBPFAMPF00294PfkBcoord: 199..278
e-value: 7.4E-18
score: 64.9
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 15..299
e-value: 3.3E-40
score: 140.0
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 15..278
IPR031094Inositol 3-kinasePANTHERPTHR43085:SF13INOSITOL 3-KINASEcoord: 14..301
NoneNo IPR availablePANTHERPTHR43085HEXOKINASE FAMILY MEMBERcoord: 14..301
IPR002173Carbohydrate/purine kinase, PfkB, conserved sitePROSITEPS00584PFKB_KINASES_2coord: 237..250

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G012840.1Lsi02G012840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0010264 myo-inositol hexakisphosphate biosynthetic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0048046 apoplast
cellular_component GO:0005773 vacuole
molecular_function GO:0005524 ATP binding
molecular_function GO:0004565 beta-galactosidase activity
molecular_function GO:0019140 inositol 3-kinase activity
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor