Lsi02G012500 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G012500
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionExocyst subunit Exo70 family protein
Locationchr02: 16449094 .. 16451004 (+)
RNA-Seq ExpressionLsi02G012500
SyntenyLsi02G012500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGACAACGGCGAGGAGAAGTTGCTGGCTATGGCTCGCCACATAGCCAAGACTATGGGTCGCAACGAGTCCATGGCCGATGATATTCTTCAGATTTTTTCCAATTTTGATGCTAGGTTTTCCCTTGATAAGTTGTCTGATAAAGCTGATGAACTTGATCCCAGAGCCCCCGCTGCTTTGCAACGCTCTCTCAAGTCTTTGGACCGCCGGATCTCCCAATACTTGGCTGCCGACCACCCCATTTGGGCTGACTCCGCCGATTCCTCTGCTTTTCTTGATGCTATCGATGAGTTGATGGCTATCATCAGGGATTGGGCGCCTATGGCTAGGGATAAGTCTGTTGCCGCCTACCTTGCACGCGCCGACGATCTGATGCAGCAGGCTATGTTCCGTGTCGACCAAGAGTTCCGGTCGTTGATGGACCGTGGAGGTGAGTCTTTTGAATTGACTCGCCATTTCAGGAACGGTGAGTCAACGGGGGATTTCTGCTTCGATTCTGAGGAAGATGAGGACGGTGCGGAAGGAATTCTTGGCGATGGCAATGCTCTCCAGATCCCTGTGGCGCAGCCGGTGACGGACTACAACATCGTTATTGATGCGCTCCCGTCGGGGACTATCAGCGATCTCCATGAAATAGCGAAGCGTATGGTGGCGGCTGGGTTCGAGAAAGAGTGTTCACACGCTTACAGTAGCTGCAGGAGGGAGTTCTTGGAGGAGAGTCTTTCTAGGTTGGGGTTACAGAAGCTGAGCATCGAGGAAGTTCAAAAGATGCAATGGCATGACCTTGAAGAGGAGATTGAACGCTGGATGAAAGCGATTACCGTCTCCCTTCGAATTCTCTTCCCAAGTGAACGACGCCTCTGCGAACGCGTCTTCGTCGGCCTTTCCTCCACCGCCGATCTTTCTTTCATGGAGGTCTGCCGTGGATCGACTATTCAGCTATTGAATTTTGCAGATGCGGTAGCAATTGGCTCCCGCGCTCCGGAACGACTTTTCAAAATCCTCGATATGTTCGAGACCTTGAGAGACTTGATGCCAGAGTTCGATTCAATATTTTCAGACCAGTACTGCTTGTTGCTTAGAAATGAAGCAATTACAATCTGGAAACGGTTAGGCGGAACAATCAAAGGGATCTTCATGGAGCTTGAGAATTTGATCCGCCGTGATCCGGCTAAAACTCCGGTCCCAGGGGGCGGCCTTCATCCAATCACTCGATATGTGATGAATTACCTTAGAGCAGCCTGTAAGTCGCGGCAGACTTTGGAGCAAGTTTTCGAAGAAGCTGCTCTTCCCTCTAAGGATTACACCAAGCTCGAGGACAGAGCAGCAGCATCGTCTTCCTTATCAGTTCAAATGGACTGGATTATGGAGCTTTTAGAGAGCAATTTAGAGGCGAAATCGAAGATATACAAGGATCTTTCCTTAAGCTCTGTGTTTCTGATGAACAATGGGCGATACATTGTTCAGAAAGTGAAAGACAGTGAATTAGGATCAGTTTTAGGCGACGATTGGATTCGAAAACACTCGGTTAAAAACCGGCAATACCTTGGAAACTACCTGAAAAGCTCATGGAGCAAGGTGGTCGGAGCATTGAAAATGGACAGTGGCACTTTAGCTCCGGCTGTGATGAAGGAGAAGCTTCAATCATTCAATATGCAGTTTGAAGAAATCTGCCAAACCCAGTCCACATGGGTGATATTTGAACATCAGCTTAAAGAAGAAGCAAGAATCTCAGTTGCCAAAATCCTGTTGCCAGCATATCAAAAGTTCATAGGAAGATACCAGAGTTTGCCGGAGTTGGCAAAGCGCACAGACCGGTATTTGAAATACACGGCGGAGGATATGGAGGCTCGAATCACCGAGCTGTTCGAAGGTGGCGGCGGCGGCTCGGGCAGTGGAAGGAGGTAA

mRNA sequence

ATGGTTGACAACGGCGAGGAGAAGTTGCTGGCTATGGCTCGCCACATAGCCAAGACTATGGGTCGCAACGAGTCCATGGCCGATGATATTCTTCAGATTTTTTCCAATTTTGATGCTAGGTTTTCCCTTGATAAGTTGTCTGATAAAGCTGATGAACTTGATCCCAGAGCCCCCGCTGCTTTGCAACGCTCTCTCAAGTCTTTGGACCGCCGGATCTCCCAATACTTGGCTGCCGACCACCCCATTTGGGCTGACTCCGCCGATTCCTCTGCTTTTCTTGATGCTATCGATGAGTTGATGGCTATCATCAGGGATTGGGCGCCTATGGCTAGGGATAAGTCTGTTGCCGCCTACCTTGCACGCGCCGACGATCTGATGCAGCAGGCTATGTTCCGTGTCGACCAAGAGTTCCGGTCGTTGATGGACCGTGGAGGTGAGTCTTTTGAATTGACTCGCCATTTCAGGAACGGTGAGTCAACGGGGGATTTCTGCTTCGATTCTGAGGAAGATGAGGACGGTGCGGAAGGAATTCTTGGCGATGGCAATGCTCTCCAGATCCCTGTGGCGCAGCCGGTGACGGACTACAACATCGTTATTGATGCGCTCCCGTCGGGGACTATCAGCGATCTCCATGAAATAGCGAAGCGTATGGTGGCGGCTGGGTTCGAGAAAGAGTGTTCACACGCTTACAGTAGCTGCAGGAGGGAGTTCTTGGAGGAGAGTCTTTCTAGGTTGGGGTTACAGAAGCTGAGCATCGAGGAAGTTCAAAAGATGCAATGGCATGACCTTGAAGAGGAGATTGAACGCTGGATGAAAGCGATTACCGTCTCCCTTCGAATTCTCTTCCCAAGTGAACGACGCCTCTGCGAACGCGTCTTCGTCGGCCTTTCCTCCACCGCCGATCTTTCTTTCATGGAGGTCTGCCGTGGATCGACTATTCAGCTATTGAATTTTGCAGATGCGGTAGCAATTGGCTCCCGCGCTCCGGAACGACTTTTCAAAATCCTCGATATGTTCGAGACCTTGAGAGACTTGATGCCAGAGTTCGATTCAATATTTTCAGACCAGTACTGCTTGTTGCTTAGAAATGAAGCAATTACAATCTGGAAACGGTTAGGCGGAACAATCAAAGGGATCTTCATGGAGCTTGAGAATTTGATCCGCCGTGATCCGGCTAAAACTCCGGTCCCAGGGGGCGGCCTTCATCCAATCACTCGATATGTGATGAATTACCTTAGAGCAGCCTGTAAGTCGCGGCAGACTTTGGAGCAAGTTTTCGAAGAAGCTGCTCTTCCCTCTAAGGATTACACCAAGCTCGAGGACAGAGCAGCAGCATCGTCTTCCTTATCAGTTCAAATGGACTGGATTATGGAGCTTTTAGAGAGCAATTTAGAGGCGAAATCGAAGATATACAAGGATCTTTCCTTAAGCTCTGTGTTTCTGATGAACAATGGGCGATACATTGTTCAGAAAGTGAAAGACAGTGAATTAGGATCAGTTTTAGGCGACGATTGGATTCGAAAACACTCGGTTAAAAACCGGCAATACCTTGGAAACTACCTGAAAAGCTCATGGAGCAAGGTGGTCGGAGCATTGAAAATGGACAGTGGCACTTTAGCTCCGGCTGTGATGAAGGAGAAGCTTCAATCATTCAATATGCAGTTTGAAGAAATCTGCCAAACCCAGTCCACATGGGTGATATTTGAACATCAGCTTAAAGAAGAAGCAAGAATCTCAGTTGCCAAAATCCTGTTGCCAGCATATCAAAAGTTCATAGGAAGATACCAGAGTTTGCCGGAGTTGGCAAAGCGCACAGACCGGTATTTGAAATACACGGCGGAGGATATGGAGGCTCGAATCACCGAGCTGTTCGAAGGTGGCGGCGGCGGCTCGGGCAGTGGAAGGAGGTAA

Coding sequence (CDS)

ATGGTTGACAACGGCGAGGAGAAGTTGCTGGCTATGGCTCGCCACATAGCCAAGACTATGGGTCGCAACGAGTCCATGGCCGATGATATTCTTCAGATTTTTTCCAATTTTGATGCTAGGTTTTCCCTTGATAAGTTGTCTGATAAAGCTGATGAACTTGATCCCAGAGCCCCCGCTGCTTTGCAACGCTCTCTCAAGTCTTTGGACCGCCGGATCTCCCAATACTTGGCTGCCGACCACCCCATTTGGGCTGACTCCGCCGATTCCTCTGCTTTTCTTGATGCTATCGATGAGTTGATGGCTATCATCAGGGATTGGGCGCCTATGGCTAGGGATAAGTCTGTTGCCGCCTACCTTGCACGCGCCGACGATCTGATGCAGCAGGCTATGTTCCGTGTCGACCAAGAGTTCCGGTCGTTGATGGACCGTGGAGGTGAGTCTTTTGAATTGACTCGCCATTTCAGGAACGGTGAGTCAACGGGGGATTTCTGCTTCGATTCTGAGGAAGATGAGGACGGTGCGGAAGGAATTCTTGGCGATGGCAATGCTCTCCAGATCCCTGTGGCGCAGCCGGTGACGGACTACAACATCGTTATTGATGCGCTCCCGTCGGGGACTATCAGCGATCTCCATGAAATAGCGAAGCGTATGGTGGCGGCTGGGTTCGAGAAAGAGTGTTCACACGCTTACAGTAGCTGCAGGAGGGAGTTCTTGGAGGAGAGTCTTTCTAGGTTGGGGTTACAGAAGCTGAGCATCGAGGAAGTTCAAAAGATGCAATGGCATGACCTTGAAGAGGAGATTGAACGCTGGATGAAAGCGATTACCGTCTCCCTTCGAATTCTCTTCCCAAGTGAACGACGCCTCTGCGAACGCGTCTTCGTCGGCCTTTCCTCCACCGCCGATCTTTCTTTCATGGAGGTCTGCCGTGGATCGACTATTCAGCTATTGAATTTTGCAGATGCGGTAGCAATTGGCTCCCGCGCTCCGGAACGACTTTTCAAAATCCTCGATATGTTCGAGACCTTGAGAGACTTGATGCCAGAGTTCGATTCAATATTTTCAGACCAGTACTGCTTGTTGCTTAGAAATGAAGCAATTACAATCTGGAAACGGTTAGGCGGAACAATCAAAGGGATCTTCATGGAGCTTGAGAATTTGATCCGCCGTGATCCGGCTAAAACTCCGGTCCCAGGGGGCGGCCTTCATCCAATCACTCGATATGTGATGAATTACCTTAGAGCAGCCTGTAAGTCGCGGCAGACTTTGGAGCAAGTTTTCGAAGAAGCTGCTCTTCCCTCTAAGGATTACACCAAGCTCGAGGACAGAGCAGCAGCATCGTCTTCCTTATCAGTTCAAATGGACTGGATTATGGAGCTTTTAGAGAGCAATTTAGAGGCGAAATCGAAGATATACAAGGATCTTTCCTTAAGCTCTGTGTTTCTGATGAACAATGGGCGATACATTGTTCAGAAAGTGAAAGACAGTGAATTAGGATCAGTTTTAGGCGACGATTGGATTCGAAAACACTCGGTTAAAAACCGGCAATACCTTGGAAACTACCTGAAAAGCTCATGGAGCAAGGTGGTCGGAGCATTGAAAATGGACAGTGGCACTTTAGCTCCGGCTGTGATGAAGGAGAAGCTTCAATCATTCAATATGCAGTTTGAAGAAATCTGCCAAACCCAGTCCACATGGGTGATATTTGAACATCAGCTTAAAGAAGAAGCAAGAATCTCAGTTGCCAAAATCCTGTTGCCAGCATATCAAAAGTTCATAGGAAGATACCAGAGTTTGCCGGAGTTGGCAAAGCGCACAGACCGGTATTTGAAATACACGGCGGAGGATATGGAGGCTCGAATCACCGAGCTGTTCGAAGGTGGCGGCGGCGGCTCGGGCAGTGGAAGGAGGTAA

Protein sequence

MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAALQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLARADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGDGNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEESLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTADLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLLLRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQTLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVFLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLAPAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLPELAKRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR
Homology
BLAST of Lsi02G012500 vs. ExPASy Swiss-Prot
Match: Q9FGH9 (Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=1 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 1.9e-243
Identity = 426/631 (67.51%), Postives = 517/631 (81.93%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           M +NGEEKLLA+ARHIAKT+G NESMADDILQIFSNFD RFS +KL++     D    A 
Sbjct: 1   MAENGEEKLLAVARHIAKTLGHNESMADDILQIFSNFDGRFSREKLAEGQAGEDGSGVAT 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           L+R+L S+D +IS+++AAD PIWAD ADS+AFLD IDEL+AIIR+W+PMA +K +   L 
Sbjct: 61  LERALNSIDGQISRFVAADQPIWADPADSAAFLDTIDELVAIIREWSPMASEKPIGICLT 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADD+MQQAMFR+++EFRSLM+RG ESF L      G    +  FDSEE+ED  +    +
Sbjct: 121 RADDMMQQAMFRIEEEFRSLMERGAESFGLNPQGDAGAM--NHRFDSEEEEDD-DRDFNN 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G+ +QIPVAQP+TDY+++IDALPS TI+DLHE+AKRM+ AGF K CSH YSSCRREFLEE
Sbjct: 181 GDDIQIPVAQPLTDYDLIIDALPSATINDLHEMAKRMLGAGFGKACSHVYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           S+SRLGLQKLSIEEV KM W +LE+EI+RW+KA  V+LRILFPSERRLC+RVF G SS A
Sbjct: 241 SMSRLGLQKLSIEEVHKMPWQELEDEIDRWIKAANVALRILFPSERRLCDRVFFGFSSAA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           DLSFMEVCRGSTIQLLNFADA+AIGSR+PERLFK+LD+FET+RDLMPEF+S+FSDQ+C +
Sbjct: 301 DLSFMEVCRGSTIQLLNFADAIAIGSRSPERLFKVLDVFETMRDLMPEFESVFSDQFCSV 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEA+TIWKRLG  I+GIFMELENLIRRDPAK  VPGGGLHPITRYVMNYLRAAC+SRQ
Sbjct: 361 LRNEAVTIWKRLGEAIRGIFMELENLIRRDPAKAAVPGGGLHPITRYVMNYLRAACRSRQ 420

Query: 421 TLEQVFEEA-ALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSV 480
           TLEQVFEE+  +PSKD          S+ L+VQM WIMELLESNLE KSK+YKD +L  V
Sbjct: 421 TLEQVFEESNGVPSKD----------STLLTVQMSWIMELLESNLEVKSKVYKDPALCYV 480

Query: 481 FLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTL 540
           FLMNNGRYIVQKVKD +LG +LGDDWIRKH+VK +QY  NY +SSW+K++G LK+D+   
Sbjct: 481 FLMNNGRYIVQKVKDGDLGLLLGDDWIRKHNVKVKQYHMNYQRSSWNKMLGLLKVDNTAA 540

Query: 541 A----PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRY 600
                   MKEKL+ FN+QF+EIC+  STWV+F+ QLKEE +IS+A++L+PAY  FIGR+
Sbjct: 541 GMNGLGKTMKEKLKQFNIQFDEICKVHSTWVVFDEQLKEELKISLARLLVPAYGSFIGRF 600

Query: 601 QSLPELAKRTDRYLKYTAEDMEARITELFEG 627
           Q+L ++ K  D+Y+KY  ED+EARI ELF+G
Sbjct: 601 QNLGDIGKNADKYIKYGVEDIEARINELFKG 618

BLAST of Lsi02G012500 vs. ExPASy Swiss-Prot
Match: Q9LMJ4 (Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=1 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 4.7e-173
Identity = 331/635 (52.13%), Postives = 432/635 (68.03%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           M + G+E L A AR IA+ +G++ S A DILQI S + A  +           DPR   +
Sbjct: 1   MAEAGDENLYAAARDIARALGKDPSAAGDILQILSGYGASGNRGG--------DPRPTPS 60

Query: 61  -------LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDK 120
                    R+L SL+R+IS Y+  D PIW+D  DS  FLD++DEL+AI  D   MA DK
Sbjct: 61  RGGSNVNFDRALTSLERQISSYIVEDRPIWSDPVDSRTFLDSVDELLAIAGDLRSMAGDK 120

Query: 121 SVAAYLARADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDG 180
           SVA   +RAD+L+QQ MFR+ +EF  +MDR  +SF+            D  F  EED D 
Sbjct: 121 SVAVCQSRADELIQQVMFRLQEEFGFVMDRAPDSFD-----------SDDEFPGEEDNDT 180

Query: 181 AEGILGDGNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSC 240
           ++G++         VA+P+TDY IVI+AL S  I DL+ IA RMVA GF KECS  YSS 
Sbjct: 181 SDGVI---------VARPITDYKIVIEALQSSVIGDLNAIAVRMVAGGFAKECSRVYSSR 240

Query: 241 RREFLEESLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVF 300
           RREFLEESLSRL L+ LS+EEVQ+  W DLE+EI+RW+KA+T+   + FPSER LC+RVF
Sbjct: 241 RREFLEESLSRLHLRGLSMEEVQESPWQDLEDEIDRWIKAVTLIFHVFFPSERLLCDRVF 300

Query: 301 VGL--SSTADLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDS 360
             L  SS  DLSFMEVCRG+T QLLNFADA+A+GSR PERLFK++D++E ++DL+P+ ++
Sbjct: 301 SDLPVSSVTDLSFMEVCRGTTTQLLNFADAIALGSRLPERLFKVVDLYEAMQDLIPKMET 360

Query: 361 IFSDQYCLLLRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNY 420
           +FSD+YC  LR+EA+ I KRLG  I+GIFMELENLIRRDP KT  PGGG+HPITRYVMNY
Sbjct: 361 LFSDRYCSPLRHEALAIHKRLGEAIRGIFMELENLIRRDPPKTAFPGGGIHPITRYVMNY 420

Query: 421 LRAACKSRQTLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIY 480
           LRAACKSRQ+LEQ+ ++            +  + +  LSVQ+ W++ELLESNLE K + Y
Sbjct: 421 LRAACKSRQSLEQILDQTG---------NETGSDTRPLSVQIIWVLELLESNLEGKKRTY 480

Query: 481 KDLSLSSVFLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGA 540
           +D SL  +F+MNN +YI+ K KD+ELG VLG+DWI KH+ K RQY  NY +SSW++VVG 
Sbjct: 481 RDPSLCFLFMMNNDKYILDKAKDNELGLVLGEDWIVKHAAKLRQYHSNYRRSSWNQVVGL 540

Query: 541 LKMDSGTLAPAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKF 600
           L+ D G     V  E L+ F  QF+E+C+ QS WV+ + QL+EE R SVA I+ PAY  F
Sbjct: 541 LRTD-GPYPKLV--ENLRLFKSQFDEVCKVQSQWVVSDGQLREELRSSVAGIVSPAYSNF 595

Query: 601 IGRYQSLPEL-AKRTDRYLKYTAEDMEARITELFE 626
           I R +  PE+  +R + ++ YT ED+E  I  LF+
Sbjct: 601 IRRLKESPEINGRRGEPFIPYTVEDVEFIIKRLFK 595

BLAST of Lsi02G012500 vs. ExPASy Swiss-Prot
Match: Q9LZD3 (Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=1 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 9.7e-94
Identity = 213/647 (32.92%), Postives = 371/647 (57.34%), Query Frame = 0

Query: 2   VDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDP----RA 61
           VD+  + L   A  +  ++ +++++ D+++ I  +FD+R S  + + +  ++      +A
Sbjct: 3   VDSRMDLLSERAVLMRASLQKSQTITDNVVSILGSFDSRLSALETAMRPTQIRTHAIRKA 62

Query: 62  PAALQRSLKSLDRRISQY----LAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDK 121
              + R+LK+ +  +SQ+     A    +     D  ++LDAI +L  IIR +      K
Sbjct: 63  HENIDRTLKAAEVILSQFDLLRQAETKVLKGPHEDLESYLDAIAQLRKIIRYFMSNKSFK 122

Query: 122 SVAAYLARADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDG 181
           S    L  A+ L+ +A  ++++EF+ L+    ++ E  R F   +   +    S + + G
Sbjct: 123 SSDGVLNHANSLLAKAQSKLEEEFKQLLASYSKAVEPDRLF---DGLPNSLRPSSDGDGG 182

Query: 182 AEGILGDGNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSC 241
            +   G  N      A     Y + I  +PS  +  LH++A++MV AG +++    Y   
Sbjct: 183 GKPHGGHHNDDAETAA-----YTLPI-LIPSRVLPLLHDLAQQMVQAGHQQQLLQIYRDT 242

Query: 242 RREFLEESLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVF 301
           R   LEESL +LG++KLS E+VQ+MQW  LE +I  W+  + +++++LF  ER++C+++F
Sbjct: 243 RSFVLEESLKKLGVEKLSKEDVQRMQWEVLEAKIGNWIHFMRIAVKLLFAGERQVCDQIF 302

Query: 302 VGLSSTADLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIF 361
            G  S +D  F EV   S   LL+F DA+A   R+PE+LF +LDM+E +R+L  E ++IF
Sbjct: 303 RGFDSLSDQCFAEVTVSSVSMLLSFGDAIARSKRSPEKLFVLLDMYEIMRELHTEIETIF 362

Query: 362 SDQYCLLLRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLR 421
             + CL +R+ A  + KRL  T +  F + E  + +D  KT V  G +HP+T YV+NY++
Sbjct: 363 KGKACLEIRDSATGLTKRLAQTAQETFGDFEEAVEKDATKTAVLDGTVHPLTSYVINYVK 422

Query: 422 AACKSRQTLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKD 481
                + TL+Q+F E       +   +D  +  +S++++   IM+ L++NL+ KSK YKD
Sbjct: 423 FLFDYQTTLKQLFLE-------FGNGDDSNSQLASVTMR---IMQALQNNLDGKSKQYKD 482

Query: 482 LSLSSVFLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVV---- 541
            +L+ +FLMNN  Y+V+ V+ SE   +LGDDW+++H    +Q+   Y + +W+K++    
Sbjct: 483 PALTHLFLMNNIHYMVRSVRRSEAKDLLGDDWVQRHRRIVQQHANQYKRVAWTKILQSSS 542

Query: 542 ---------GALK-MDSGTLAPAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARIS 601
                    G+L+  +S  ++  ++KE+ + FNMQF+E+ Q QS W + + +L+E  R++
Sbjct: 543 AQGLTSSGGGSLEGGNSSGVSRGLLKERFKMFNMQFDELHQRQSQWTVPDTELRESLRLA 602

Query: 602 VAKILLPAYQKFIGRYQSLPELAKRTDRYLKYTAEDMEARITELFEG 627
           VA++LLPAY+ F+ R+  L E  K   +Y+KYTAED+E  + ELFEG
Sbjct: 603 VAEVLLPAYRSFLKRFGPLVESGKNPQKYIKYTAEDLERLLGELFEG 630

BLAST of Lsi02G012500 vs. ExPASy Swiss-Prot
Match: F4KG58 (Exocyst complex component EXO70A3 OS=Arabidopsis thaliana OX=3702 GN=EXO70A3 PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.0e-70
Identity = 152/465 (32.69%), Postives = 260/465 (55.91%), Query Frame = 0

Query: 170 DEDGAEGILGDGNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHA 229
           D++G++G   D  +  +      TDY  V   +P   +  LH++A++MV AG ++E    
Sbjct: 129 DDEGSDGKSHDPQSNGL----GKTDYT-VPTIIPPTVLPVLHDLAQQMVKAGHQQELFKT 188

Query: 230 YSSCRREFLEESLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLC 289
           Y   RR  L +SL +LG+++ S  +V++M     E +I  W+  I +S+++LF +E+ +C
Sbjct: 189 YRDIRRAVLAQSLEKLGVERHSKYDVERMNQDVFEAKIMNWIHYIRISVKLLFAAEKEIC 248

Query: 290 ERVFVGLSSTADLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEF 349
            ++  G+    D SF E+   S   LL+F  A+AI  R+PE++F ILDM+E + +L PEF
Sbjct: 249 HQILDGVEPFRDQSFAEITTISFGMLLSFGYAIAISRRSPEKVFVILDMYEIMIELQPEF 308

Query: 350 DSIFSDQYCLLLRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVM 409
           + IF  + C  ++ +A+ + K L  T+K    + E  I  D  +T V  G +H +T YV 
Sbjct: 309 ELIFGSKPCTEMKEDALNLTKLLAQTVKETIADFEVAIEMDATETVVMDGSVHALTSYVA 368

Query: 410 NYLRAACKSRQTLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSK 469
            Y++       TL Q+F+E      D TKL+            M  IM  L +NL+ KS+
Sbjct: 369 RYVKFLFDYEPTLRQLFQEFNSNDPD-TKLKS----------VMTGIMRALRNNLDGKSR 428

Query: 470 IYKDLSLSSVFLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVV 529
            ++D +L+ +FLMNN  YIV+  +  E  + LGDD ++ H    +Q+   Y   SW+K++
Sbjct: 429 QFEDAALTQLFLMNNVYYIVRNFRREEAKNFLGDDLVQTHRRIVQQHAKQYQTISWNKIL 488

Query: 530 GALKMDSG--------TLAPAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVA 589
             + + S         ++   ++KEK ++FN QFEE+ Q Q  W + + +L+E  R+++A
Sbjct: 489 QCITVQSSKSGLIKNESIKKTLVKEKFKTFNSQFEELHQRQCQWSVSDVELRESLRLAIA 548

Query: 590 KILLPAYQKFIGRYQSLPELAKRTDRYLKYTAEDMEARITELFEG 627
           ++LLPAY  F+ R+  + E  K + +Y+++T ED+E  + + F+G
Sbjct: 549 EVLLPAYGSFLKRFGPMIESGKNSQKYIRFTPEDLERMLNDFFQG 577

BLAST of Lsi02G012500 vs. ExPASy Swiss-Prot
Match: Q8VY27 (Exocyst complex component EXO70H1 OS=Arabidopsis thaliana OX=3702 GN=EXO70H1 PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 5.2e-63
Identity = 151/516 (29.26%), Postives = 274/516 (53.10%), Query Frame = 0

Query: 119 LARADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSE-EDEDGAEGI 178
           LA A  LMQ AM R+++EF  ++    +  +L     +G+S+     +SE ED  G++  
Sbjct: 104 LALAQTLMQIAMARLEKEFFQILSSNRD--KLDPESVSGQSS--ISSNSEFEDVMGSDDD 163

Query: 179 LGDGNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREF 238
               N ++    + +T     ++   +  +SDL  IA+ M++ G+ KEC   Y   R+  
Sbjct: 164 DESDNEMR-KAGESITQ----VEKASAMVMSDLKAIAESMISCGYGKECVKIYKRVRKSI 223

Query: 239 LEESLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLS 298
           ++E LS LG++        +  W  LE  I+ W+KA  + +  LF  E+ LC+ VF   +
Sbjct: 224 VDEGLSLLGIEIYKGSRFHRTDWVTLEHMIKNWIKAAKIGIATLFRGEKLLCDHVFSASN 283

Query: 299 STADLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQY 358
           ST +  F E+   +   L  F + VA   ++ ER+F ++D+   + DL  + + IF    
Sbjct: 284 STRESCFYEIANEAATNLFKFPEFVAKEKKSHERIFPLMDLQAAISDLWQDIEMIFHFDA 343

Query: 359 CLLLRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACK 418
              ++++A+T  ++L  +I     + E++I++D  K   PGGG+H +TR  MN++ +  K
Sbjct: 344 VAGVKSQALTSLQKLKVSIHSALTDFESIIQKDTTKALTPGGGIHKLTRSTMNFISSLSK 403

Query: 419 SRQTLEQVFEEAALPS-----KDYTK--LEDRAAASSSLSVQMDWIMELLESNLEAKSKI 478
               L ++  +  LP      + Y +  + +    + +LSV   W++ +L   L+ K++ 
Sbjct: 404 YSGVLSEILADHPLPRNTRLLESYVRAPISEDEQHNHALSVHFAWLILVLLCKLDTKAEH 463

Query: 479 YKDLSLSSVFLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVG 538
           YKD+SLS +FL NN + I++ V  + L ++LGDDW+ KH  K   Y GNY  ++WS V  
Sbjct: 464 YKDVSLSYLFLANNLQIIIETVGSTPLRNLLGDDWLNKHEDKLCAYAGNYEIAAWSNVFM 523

Query: 539 ALKMDSGTLAPAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQK 598
           +L  +   L+P   K   + F+  FEE    QS+ V+   +L++E ++S+AK L+P Y++
Sbjct: 524 SLPEEPTDLSPEEAKIYFRRFHTAFEEAYMKQSSRVVPNAKLRDELKVSIAKKLVPEYRE 583

Query: 599 FIGRYQSLPELAKRTDRYLKYTAEDMEARITELFEG 627
           F  +Y  +    +  +  +++  +++E  I++LF G
Sbjct: 584 FYRKYLPMLGQERNIEILVRFKPDNLENYISDLFHG 610

BLAST of Lsi02G012500 vs. ExPASy TrEMBL
Match: A0A0A0LVL7 (Exocyst subunit Exo70 family protein OS=Cucumis sativus OX=3659 GN=Csa_1G533450 PE=3 SV=1)

HSP 1 Score: 1186.0 bits (3067), Expect = 0.0e+00
Identity = 603/636 (94.81%), Postives = 623/636 (97.96%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSL+KLSDK DELDPRAP A
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLEKLSDKPDELDPRAPTA 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           LQRSLKSLDRRISQYLAADHPIWADSADSSAFLD+IDELM IIRDWAPMARDKSVA+YLA
Sbjct: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDSIDELMGIIRDWAPMARDKSVASYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDE+ AEGILGD
Sbjct: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEEVAEGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G+ +QIPVAQPVTDYNI+IDALPSGTI+DLHEIAKRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 GDGIQIPVAQPVTDYNILIDALPSGTINDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQW DLEEEIERWMKAIT+SLRILFPSERRLCERVFVGLS+TA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWQDLEEEIERWMKAITLSLRILFPSERRLCERVFVGLSTTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDS+FSDQYCLL
Sbjct: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSVFSDQYCLL 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYL+AACKSRQ
Sbjct: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLKAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF 480
           TLEQVF+E ALPSKDYTK +DRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF
Sbjct: 421 TLEQVFDEPALPSKDYTKFDDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF 480

Query: 481 LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540
           LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA
Sbjct: 481 LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540

Query: 541 PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLPE 600
           P+ MKEKLQSFNMQFEEICQTQSTWVIFE+QL+EE RISVAKILLPAYQKFIGRYQSLPE
Sbjct: 541 PSAMKEKLQSFNMQFEEICQTQSTWVIFENQLREETRISVAKILLPAYQKFIGRYQSLPE 600

Query: 601 LAKRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR 637
           LAKRTDRYLKYTAE+ME+RITELFE  GG SGSGRR
Sbjct: 601 LAKRTDRYLKYTAEEMESRITELFE--GGSSGSGRR 634

BLAST of Lsi02G012500 vs. ExPASy TrEMBL
Match: A0A5A7V2N9 (Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00730 PE=3 SV=1)

HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 598/636 (94.03%), Postives = 617/636 (97.01%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDK DELDPRAP A
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKPDELDPRAPTA 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           LQRSLKSLDRRISQYLAADHPIWADSADSSAFLD+IDEL+ IIRDWAPMARDKSVA+YLA
Sbjct: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDSIDELLGIIRDWAPMARDKSVASYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDE+GAEGILGD
Sbjct: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEEGAEGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G+ +QIPVAQPVTDYNI+IDALPSGTI+DLHEIAKRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 GDGIQIPVAQPVTDYNILIDALPSGTINDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQW DLEEEIERWMKAIT+SLRILFPSERRLCERVFVGLS+TA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWQDLEEEIERWMKAITLSLRILFPSERRLCERVFVGLSTTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDS+FSDQYCLL
Sbjct: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSVFSDQYCLL 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ
Sbjct: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF 480
           TLEQVF+E ALPS       +RAAASSSLSVQM+WIMELLESNLEAKSKIYKDLSLSSVF
Sbjct: 421 TLEQVFDEPALPS-------ERAAASSSLSVQMEWIMELLESNLEAKSKIYKDLSLSSVF 480

Query: 481 LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540
           LMNNGRYIVQKVKDS+LGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA
Sbjct: 481 LMNNGRYIVQKVKDSDLGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540

Query: 541 PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLPE 600
           P VMKEKLQSFNMQFEEICQTQSTWVIFE+QL+EE RISVAKILLPAYQKFIGRYQSLPE
Sbjct: 541 PTVMKEKLQSFNMQFEEICQTQSTWVIFENQLREETRISVAKILLPAYQKFIGRYQSLPE 600

Query: 601 LAKRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR 637
           LAKRTDRYLKYTAE+MEARI ELFE  GG SGSGRR
Sbjct: 601 LAKRTDRYLKYTAEEMEARINELFE--GGNSGSGRR 627

BLAST of Lsi02G012500 vs. ExPASy TrEMBL
Match: A0A1S3C4U4 (Exocyst subunit Exo70 family protein OS=Cucumis melo OX=3656 GN=LOC103497050 PE=3 SV=1)

HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 598/636 (94.03%), Postives = 617/636 (97.01%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDK DELDPRAP A
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKPDELDPRAPTA 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           LQRSLKSLDRRISQYLAADHPIWADSADSSAFLD+IDEL+ IIRDWAPMARDKSVA+YLA
Sbjct: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDSIDELLGIIRDWAPMARDKSVASYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDE+GAEGILGD
Sbjct: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEEGAEGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G+ +QIPVAQPVTDYNI+IDALPSGTI+DLHEIAKRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 GDGIQIPVAQPVTDYNILIDALPSGTINDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQW DLEEEIERWMKAIT+SLRILFPSERRLCERVFVGLS+TA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWQDLEEEIERWMKAITLSLRILFPSERRLCERVFVGLSTTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDS+FSDQYCLL
Sbjct: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSVFSDQYCLL 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ
Sbjct: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF 480
           TLEQVF+E ALPS       +RAAASSSLSVQM+WIMELLESNLEAKSKIYKDLSLSSVF
Sbjct: 421 TLEQVFDEPALPS-------ERAAASSSLSVQMEWIMELLESNLEAKSKIYKDLSLSSVF 480

Query: 481 LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540
           LMNNGRYIVQKVKDS+LGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA
Sbjct: 481 LMNNGRYIVQKVKDSDLGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540

Query: 541 PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLPE 600
           P VMKEKLQSFNMQFEEICQTQSTWVIFE+QL+EE RISVAKILLPAYQKFIGRYQSLPE
Sbjct: 541 PTVMKEKLQSFNMQFEEICQTQSTWVIFENQLREETRISVAKILLPAYQKFIGRYQSLPE 600

Query: 601 LAKRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR 637
           LAKRTDRYLKYTAE+MEARI ELFE  GG SGSGRR
Sbjct: 601 LAKRTDRYLKYTAEEMEARINELFE--GGNSGSGRR 627

BLAST of Lsi02G012500 vs. ExPASy TrEMBL
Match: A0A6J1HK70 (Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC111464860 PE=3 SV=1)

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 584/638 (91.54%), Postives = 606/638 (94.98%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDIL IFSNFDARFSLDKLSDK DELDPR  A 
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILHIFSNFDARFSLDKLSDKPDELDPRGSAD 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           L RSLKSLDRRISQYL ADHPIWADSADSSAFLDAIDELMAIIRDW PMARDKSV  YLA
Sbjct: 61  LHRSLKSLDRRISQYLVADHPIWADSADSSAFLDAIDELMAIIRDWTPMARDKSVGNYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDED AEGILGD
Sbjct: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDVAEGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G  LQIPVAQPVTDYNIVIDALPSGTI DLHEI+KRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 G--LQIPVAQPVTDYNIVIDALPSGTIGDLHEISKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQWHDLEEEIERWMKAI+VSLR+LFPSER LCERVFVGLSSTA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWHDLEEEIERWMKAISVSLRVLFPSERHLCERVFVGLSSTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           +LSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKI+DMFETLRDLMPEFDS+F D YC  
Sbjct: 301 NLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKIIDMFETLRDLMPEFDSVFPDLYCSF 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEA+TIWK  GGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ
Sbjct: 361 LRNEALTIWKSFGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAA--SSSLSVQMDWIMELLESNLEAKSKIYKDLSLSS 480
           TLEQVFEE AL SKDY+KLEDRAAA  SSSLSVQM+WIME+LESNLEAKSKIYKDLSLSS
Sbjct: 421 TLEQVFEEVALTSKDYSKLEDRAAASSSSSLSVQMEWIMEVLESNLEAKSKIYKDLSLSS 480

Query: 481 VFLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGT 540
           VFLMNNGRYIVQKVKDSELGSVLG+DWIRKHSVKNRQYLGNYL+SSWSKV+G LK+DSG+
Sbjct: 481 VFLMNNGRYIVQKVKDSELGSVLGEDWIRKHSVKNRQYLGNYLRSSWSKVIGVLKVDSGS 540

Query: 541 LAPAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSL 600
           LAPA MKEKLQSFN+QFE++CQTQSTWVIFEHQL+EEARISVAK LLPAYQKF+GRYQSL
Sbjct: 541 LAPAAMKEKLQSFNVQFEQVCQTQSTWVIFEHQLREEARISVAKTLLPAYQKFVGRYQSL 600

Query: 601 PELAKRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR 637
           PELAKRTDRYLKYT ED+E RITELFEGGGGG G GRR
Sbjct: 601 PELAKRTDRYLKYTVEDVEGRITELFEGGGGGGGGGRR 636

BLAST of Lsi02G012500 vs. ExPASy TrEMBL
Match: A0A6J1I2X0 (Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111469428 PE=3 SV=1)

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 582/636 (91.51%), Postives = 605/636 (95.13%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDIL IFSNFDARFSLDKLSDK DELDPR  A 
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILHIFSNFDARFSLDKLSDKPDELDPRGSAD 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           L RSLKSLDRRISQYL ADHPIWADSADSSAFLDAIDELMAIIRDW PMARDKSV  YLA
Sbjct: 61  LHRSLKSLDRRISQYLVADHPIWADSADSSAFLDAIDELMAIIRDWTPMARDKSVGNYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAM RVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDED AEGILGD
Sbjct: 121 RADDLMQQAMLRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDVAEGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G  LQIPVAQPVTDYNIVIDALPSGTI DLHEIAKRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 G--LQIPVAQPVTDYNIVIDALPSGTIGDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQWHDLEEEIERWMKAI+VSLR+LFPSER LCERVFVGLSSTA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWHDLEEEIERWMKAISVSLRVLFPSERHLCERVFVGLSSTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           +LSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKI+DMFETLRDLMPEFDS+F DQYC  
Sbjct: 301 NLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKIIDMFETLRDLMPEFDSVFPDQYCSF 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEA+TIWK  GGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ
Sbjct: 361 LRNEALTIWKSFGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAA-SSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSV 480
           TLEQ+FEE AL SKDY+KLEDRAAA SSSLSVQM+WIME+LESNLEAKSKIYKDLSLSSV
Sbjct: 421 TLEQIFEEVALTSKDYSKLEDRAAASSSSLSVQMEWIMEVLESNLEAKSKIYKDLSLSSV 480

Query: 481 FLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTL 540
           FLMNNGRYIVQKVKDSELGSVLG+DWIRKHSVKNRQYLGNYL+SSWSKV+G LKM+SG+L
Sbjct: 481 FLMNNGRYIVQKVKDSELGSVLGEDWIRKHSVKNRQYLGNYLRSSWSKVIGVLKMESGSL 540

Query: 541 APAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLP 600
           APA MK+KLQSFN+QFE++CQTQSTWVIFEHQL+EEARISVAK LLPAYQKF+GRYQSLP
Sbjct: 541 APAAMKDKLQSFNVQFEQVCQTQSTWVIFEHQLREEARISVAKTLLPAYQKFVGRYQSLP 600

Query: 601 ELAKRTDRYLKYTAEDMEARITELFEGGGGGSGSGR 636
           ELAKRTDRYLKYT ED+E RITELFEGGGGG GS R
Sbjct: 601 ELAKRTDRYLKYTVEDVEGRITELFEGGGGG-GSRR 633

BLAST of Lsi02G012500 vs. NCBI nr
Match: XP_038894987.1 (exocyst complex component EXO70B1-like [Benincasa hispida])

HSP 1 Score: 1201.8 bits (3108), Expect = 0.0e+00
Identity = 613/636 (96.38%), Postives = 626/636 (98.43%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDK DELDPRAPAA
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKPDELDPRAPAA 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPM++D SVA YLA
Sbjct: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMSKDNSVATYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEED+   EGILGD
Sbjct: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDD---EGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G+ALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 GDALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQW DLEEEIERWMKAITVSLRILFPSERRLCERVFVGLS+TA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWQDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSTTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDS+FSDQYCLL
Sbjct: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSVFSDQYCLL 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ
Sbjct: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF 480
           TLEQVFEEA+LPSKDYTKL+DRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF
Sbjct: 421 TLEQVFEEASLPSKDYTKLDDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF 480

Query: 481 LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540
           LMNNGRYIVQKVKDSELG VLGDDWIRKHSVKNRQYLGNYLK SWSKV+GALK+DSGTLA
Sbjct: 481 LMNNGRYIVQKVKDSELGLVLGDDWIRKHSVKNRQYLGNYLKGSWSKVIGALKVDSGTLA 540

Query: 541 PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLPE 600
           PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQL+EEARISVAKILLPAYQKFI RYQSLPE
Sbjct: 541 PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLREEARISVAKILLPAYQKFIARYQSLPE 600

Query: 601 LAKRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR 637
           L+KRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR
Sbjct: 601 LSKRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR 633

BLAST of Lsi02G012500 vs. NCBI nr
Match: XP_004154337.1 (exocyst complex component EXO70B1 [Cucumis sativus] >KGN65838.1 hypothetical protein Csa_023294 [Cucumis sativus])

HSP 1 Score: 1186.0 bits (3067), Expect = 0.0e+00
Identity = 603/636 (94.81%), Postives = 623/636 (97.96%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSL+KLSDK DELDPRAP A
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLEKLSDKPDELDPRAPTA 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           LQRSLKSLDRRISQYLAADHPIWADSADSSAFLD+IDELM IIRDWAPMARDKSVA+YLA
Sbjct: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDSIDELMGIIRDWAPMARDKSVASYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDE+ AEGILGD
Sbjct: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEEVAEGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G+ +QIPVAQPVTDYNI+IDALPSGTI+DLHEIAKRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 GDGIQIPVAQPVTDYNILIDALPSGTINDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQW DLEEEIERWMKAIT+SLRILFPSERRLCERVFVGLS+TA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWQDLEEEIERWMKAITLSLRILFPSERRLCERVFVGLSTTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDS+FSDQYCLL
Sbjct: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSVFSDQYCLL 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYL+AACKSRQ
Sbjct: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLKAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF 480
           TLEQVF+E ALPSKDYTK +DRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF
Sbjct: 421 TLEQVFDEPALPSKDYTKFDDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF 480

Query: 481 LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540
           LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA
Sbjct: 481 LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540

Query: 541 PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLPE 600
           P+ MKEKLQSFNMQFEEICQTQSTWVIFE+QL+EE RISVAKILLPAYQKFIGRYQSLPE
Sbjct: 541 PSAMKEKLQSFNMQFEEICQTQSTWVIFENQLREETRISVAKILLPAYQKFIGRYQSLPE 600

Query: 601 LAKRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR 637
           LAKRTDRYLKYTAE+ME+RITELFE  GG SGSGRR
Sbjct: 601 LAKRTDRYLKYTAEEMESRITELFE--GGSSGSGRR 634

BLAST of Lsi02G012500 vs. NCBI nr
Match: XP_008457332.1 (PREDICTED: exocyst complex component EXO70B1-like [Cucumis melo] >KAA0060021.1 exocyst complex component EXO70B1-like [Cucumis melo var. makuwa] >TYJ97279.1 exocyst complex component EXO70B1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 598/636 (94.03%), Postives = 617/636 (97.01%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDK DELDPRAP A
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKPDELDPRAPTA 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           LQRSLKSLDRRISQYLAADHPIWADSADSSAFLD+IDEL+ IIRDWAPMARDKSVA+YLA
Sbjct: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDSIDELLGIIRDWAPMARDKSVASYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDE+GAEGILGD
Sbjct: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEEGAEGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G+ +QIPVAQPVTDYNI+IDALPSGTI+DLHEIAKRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 GDGIQIPVAQPVTDYNILIDALPSGTINDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQW DLEEEIERWMKAIT+SLRILFPSERRLCERVFVGLS+TA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWQDLEEEIERWMKAITLSLRILFPSERRLCERVFVGLSTTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDS+FSDQYCLL
Sbjct: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSVFSDQYCLL 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ
Sbjct: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSVF 480
           TLEQVF+E ALPS       +RAAASSSLSVQM+WIMELLESNLEAKSKIYKDLSLSSVF
Sbjct: 421 TLEQVFDEPALPS-------ERAAASSSLSVQMEWIMELLESNLEAKSKIYKDLSLSSVF 480

Query: 481 LMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540
           LMNNGRYIVQKVKDS+LGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA
Sbjct: 481 LMNNGRYIVQKVKDSDLGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTLA 540

Query: 541 PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLPE 600
           P VMKEKLQSFNMQFEEICQTQSTWVIFE+QL+EE RISVAKILLPAYQKFIGRYQSLPE
Sbjct: 541 PTVMKEKLQSFNMQFEEICQTQSTWVIFENQLREETRISVAKILLPAYQKFIGRYQSLPE 600

Query: 601 LAKRTDRYLKYTAEDMEARITELFEGGGGGSGSGRR 637
           LAKRTDRYLKYTAE+MEARI ELFE  GG SGSGRR
Sbjct: 601 LAKRTDRYLKYTAEEMEARINELFE--GGNSGSGRR 627

BLAST of Lsi02G012500 vs. NCBI nr
Match: XP_023519988.1 (exocyst complex component EXO70B1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 584/635 (91.97%), Postives = 606/635 (95.43%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDIL IFSNFDARFSLDKLSDK DELDPR  A 
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILHIFSNFDARFSLDKLSDKPDELDPRGSAD 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           L RSLKSLDRRISQYL ADHPIWADSADSSAFLDAIDELMAIIRDW PMARDKSV  YLA
Sbjct: 61  LHRSLKSLDRRISQYLVADHPIWADSADSSAFLDAIDELMAIIRDWTPMARDKSVGNYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDED AEGILGD
Sbjct: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDVAEGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G  LQIPVAQPVTDYNIVIDALPSGTI DLHEIAKRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 G--LQIPVAQPVTDYNIVIDALPSGTIGDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQWHDLEEEIERWMKAI+VSLR+LFPSER LCERVFVGLSSTA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWHDLEEEIERWMKAISVSLRVLFPSERHLCERVFVGLSSTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           +LSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKI+DMFETLRDLMPEFDS+F+DQYC  
Sbjct: 301 NLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKIIDMFETLRDLMPEFDSVFADQYCSF 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEA+TIWK  GGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ
Sbjct: 361 LRNEALTIWKSFGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAA-SSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSV 480
           TLEQVFEE AL SKDY+KLEDRAAA SSSLSVQM+WIME+LESNLEAKSKIYKDLSLSSV
Sbjct: 421 TLEQVFEEVALTSKDYSKLEDRAAASSSSLSVQMEWIMEVLESNLEAKSKIYKDLSLSSV 480

Query: 481 FLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTL 540
           FLMNNGRYIVQKVKDSELGSVLG+DWIRKHSVKNRQYLGNYL+SSWSKV+G LK+DSG+L
Sbjct: 481 FLMNNGRYIVQKVKDSELGSVLGEDWIRKHSVKNRQYLGNYLRSSWSKVIGVLKVDSGSL 540

Query: 541 APAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLP 600
           APA MKEKLQSFN+QFE++CQTQSTWVIFEHQL+EEARISVAK LLPAYQKF+GRYQSLP
Sbjct: 541 APAAMKEKLQSFNVQFEQVCQTQSTWVIFEHQLREEARISVAKTLLPAYQKFVGRYQSLP 600

Query: 601 ELAKRTDRYLKYTAEDMEARITELFEGGGGGSGSG 635
           ELAKRTDRYLKYT ED+E RITELFEGGGGG G G
Sbjct: 601 ELAKRTDRYLKYTVEDVEGRITELFEGGGGGGGGG 633

BLAST of Lsi02G012500 vs. NCBI nr
Match: XP_023519989.1 (exocyst complex component EXO70B1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 584/635 (91.97%), Postives = 606/635 (95.43%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           MVDNGEEKLLAMARHIAKTMGRNESMADDIL IFSNFDARFSLDKLSDK DELDPR  A 
Sbjct: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILHIFSNFDARFSLDKLSDKPDELDPRGSAD 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           L RSLKSLDRRISQYL ADHPIWADSADSSAFLDAIDELMAIIRDW PMARDKSV  YLA
Sbjct: 61  LHRSLKSLDRRISQYLVADHPIWADSADSSAFLDAIDELMAIIRDWTPMARDKSVGNYLA 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDED AEGILGD
Sbjct: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDVAEGILGD 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G  LQIPVAQPVTDYNIVIDALPSGTI DLHEIAKRMVAAGFEKECSHAYSSCRREFLEE
Sbjct: 181 G--LQIPVAQPVTDYNIVIDALPSGTIGDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           SLSRLGLQKLSI+EVQKMQWHDLEEEIERWMKAI+VSLR+LFPSER LCERVFVGLSSTA
Sbjct: 241 SLSRLGLQKLSIDEVQKMQWHDLEEEIERWMKAISVSLRVLFPSERHLCERVFVGLSSTA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           +LSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKI+DMFETLRDLMPEFDS+F+DQYC  
Sbjct: 301 NLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKIIDMFETLRDLMPEFDSVFADQYCSF 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEA+TIWK  GGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ
Sbjct: 361 LRNEALTIWKSFGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420

Query: 421 TLEQVFEEAALPSKDYTKLEDRAAA-SSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSV 480
           TLEQVFEE AL SKDY+KLEDRAAA SSSLSVQM+WIME+LESNLEAKSKIYKDLSLSSV
Sbjct: 421 TLEQVFEEVALTSKDYSKLEDRAAASSSSLSVQMEWIMEVLESNLEAKSKIYKDLSLSSV 480

Query: 481 FLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTL 540
           FLMNNGRYIVQKVKDSELGSVLG+DWIRKHSVKNRQYLGNYL+SSWSKV+G LK+DSG+L
Sbjct: 481 FLMNNGRYIVQKVKDSELGSVLGEDWIRKHSVKNRQYLGNYLRSSWSKVIGVLKVDSGSL 540

Query: 541 APAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLP 600
           APA MKEKLQSFN+QFE++CQTQSTWVIFEHQL+EEARISVAK LLPAYQKF+GRYQSLP
Sbjct: 541 APAAMKEKLQSFNVQFEQVCQTQSTWVIFEHQLREEARISVAKTLLPAYQKFVGRYQSLP 600

Query: 601 ELAKRTDRYLKYTAEDMEARITELFEGGGGGSGSG 635
           ELAKRTDRYLKYT ED+E RITELFEGGGGG G G
Sbjct: 601 ELAKRTDRYLKYTVEDVEGRITELFEGGGGGGGGG 633

BLAST of Lsi02G012500 vs. TAIR 10
Match: AT5G58430.1 (exocyst subunit exo70 family protein B1 )

HSP 1 Score: 843.2 bits (2177), Expect = 1.4e-244
Identity = 426/631 (67.51%), Postives = 517/631 (81.93%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           M +NGEEKLLA+ARHIAKT+G NESMADDILQIFSNFD RFS +KL++     D    A 
Sbjct: 1   MAENGEEKLLAVARHIAKTLGHNESMADDILQIFSNFDGRFSREKLAEGQAGEDGSGVAT 60

Query: 61  LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDKSVAAYLA 120
           L+R+L S+D +IS+++AAD PIWAD ADS+AFLD IDEL+AIIR+W+PMA +K +   L 
Sbjct: 61  LERALNSIDGQISRFVAADQPIWADPADSAAFLDTIDELVAIIREWSPMASEKPIGICLT 120

Query: 121 RADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDGAEGILGD 180
           RADD+MQQAMFR+++EFRSLM+RG ESF L      G    +  FDSEE+ED  +    +
Sbjct: 121 RADDMMQQAMFRIEEEFRSLMERGAESFGLNPQGDAGAM--NHRFDSEEEEDD-DRDFNN 180

Query: 181 GNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSCRREFLEE 240
           G+ +QIPVAQP+TDY+++IDALPS TI+DLHE+AKRM+ AGF K CSH YSSCRREFLEE
Sbjct: 181 GDDIQIPVAQPLTDYDLIIDALPSATINDLHEMAKRMLGAGFGKACSHVYSSCRREFLEE 240

Query: 241 SLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVFVGLSSTA 300
           S+SRLGLQKLSIEEV KM W +LE+EI+RW+KA  V+LRILFPSERRLC+RVF G SS A
Sbjct: 241 SMSRLGLQKLSIEEVHKMPWQELEDEIDRWIKAANVALRILFPSERRLCDRVFFGFSSAA 300

Query: 301 DLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDSIFSDQYCLL 360
           DLSFMEVCRGSTIQLLNFADA+AIGSR+PERLFK+LD+FET+RDLMPEF+S+FSDQ+C +
Sbjct: 301 DLSFMEVCRGSTIQLLNFADAIAIGSRSPERLFKVLDVFETMRDLMPEFESVFSDQFCSV 360

Query: 361 LRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNYLRAACKSRQ 420
           LRNEA+TIWKRLG  I+GIFMELENLIRRDPAK  VPGGGLHPITRYVMNYLRAAC+SRQ
Sbjct: 361 LRNEAVTIWKRLGEAIRGIFMELENLIRRDPAKAAVPGGGLHPITRYVMNYLRAACRSRQ 420

Query: 421 TLEQVFEEA-ALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIYKDLSLSSV 480
           TLEQVFEE+  +PSKD          S+ L+VQM WIMELLESNLE KSK+YKD +L  V
Sbjct: 421 TLEQVFEESNGVPSKD----------STLLTVQMSWIMELLESNLEVKSKVYKDPALCYV 480

Query: 481 FLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGALKMDSGTL 540
           FLMNNGRYIVQKVKD +LG +LGDDWIRKH+VK +QY  NY +SSW+K++G LK+D+   
Sbjct: 481 FLMNNGRYIVQKVKDGDLGLLLGDDWIRKHNVKVKQYHMNYQRSSWNKMLGLLKVDNTAA 540

Query: 541 A----PAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRY 600
                   MKEKL+ FN+QF+EIC+  STWV+F+ QLKEE +IS+A++L+PAY  FIGR+
Sbjct: 541 GMNGLGKTMKEKLKQFNIQFDEICKVHSTWVVFDEQLKEELKISLARLLVPAYGSFIGRF 600

Query: 601 QSLPELAKRTDRYLKYTAEDMEARITELFEG 627
           Q+L ++ K  D+Y+KY  ED+EARI ELF+G
Sbjct: 601 QNLGDIGKNADKYIKYGVEDIEARINELFKG 618

BLAST of Lsi02G012500 vs. TAIR 10
Match: AT1G07000.1 (exocyst subunit exo70 family protein B2 )

HSP 1 Score: 609.4 bits (1570), Expect = 3.4e-174
Identity = 331/635 (52.13%), Postives = 432/635 (68.03%), Query Frame = 0

Query: 1   MVDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLSDKADELDPRAPAA 60
           M + G+E L A AR IA+ +G++ S A DILQI S + A  +           DPR   +
Sbjct: 1   MAEAGDENLYAAARDIARALGKDPSAAGDILQILSGYGASGNRGG--------DPRPTPS 60

Query: 61  -------LQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAIIRDWAPMARDK 120
                    R+L SL+R+IS Y+  D PIW+D  DS  FLD++DEL+AI  D   MA DK
Sbjct: 61  RGGSNVNFDRALTSLERQISSYIVEDRPIWSDPVDSRTFLDSVDELLAIAGDLRSMAGDK 120

Query: 121 SVAAYLARADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTGDFCFDSEEDEDG 180
           SVA   +RAD+L+QQ MFR+ +EF  +MDR  +SF+            D  F  EED D 
Sbjct: 121 SVAVCQSRADELIQQVMFRLQEEFGFVMDRAPDSFD-----------SDDEFPGEEDNDT 180

Query: 181 AEGILGDGNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAYSSC 240
           ++G++         VA+P+TDY IVI+AL S  I DL+ IA RMVA GF KECS  YSS 
Sbjct: 181 SDGVI---------VARPITDYKIVIEALQSSVIGDLNAIAVRMVAGGFAKECSRVYSSR 240

Query: 241 RREFLEESLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLCERVF 300
           RREFLEESLSRL L+ LS+EEVQ+  W DLE+EI+RW+KA+T+   + FPSER LC+RVF
Sbjct: 241 RREFLEESLSRLHLRGLSMEEVQESPWQDLEDEIDRWIKAVTLIFHVFFPSERLLCDRVF 300

Query: 301 VGL--SSTADLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEFDS 360
             L  SS  DLSFMEVCRG+T QLLNFADA+A+GSR PERLFK++D++E ++DL+P+ ++
Sbjct: 301 SDLPVSSVTDLSFMEVCRGTTTQLLNFADAIALGSRLPERLFKVVDLYEAMQDLIPKMET 360

Query: 361 IFSDQYCLLLRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVMNY 420
           +FSD+YC  LR+EA+ I KRLG  I+GIFMELENLIRRDP KT  PGGG+HPITRYVMNY
Sbjct: 361 LFSDRYCSPLRHEALAIHKRLGEAIRGIFMELENLIRRDPPKTAFPGGGIHPITRYVMNY 420

Query: 421 LRAACKSRQTLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSKIY 480
           LRAACKSRQ+LEQ+ ++            +  + +  LSVQ+ W++ELLESNLE K + Y
Sbjct: 421 LRAACKSRQSLEQILDQTG---------NETGSDTRPLSVQIIWVLELLESNLEGKKRTY 480

Query: 481 KDLSLSSVFLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKVVGA 540
           +D SL  +F+MNN +YI+ K KD+ELG VLG+DWI KH+ K RQY  NY +SSW++VVG 
Sbjct: 481 RDPSLCFLFMMNNDKYILDKAKDNELGLVLGEDWIVKHAAKLRQYHSNYRRSSWNQVVGL 540

Query: 541 LKMDSGTLAPAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKILLPAYQKF 600
           L+ D G     V  E L+ F  QF+E+C+ QS WV+ + QL+EE R SVA I+ PAY  F
Sbjct: 541 LRTD-GPYPKLV--ENLRLFKSQFDEVCKVQSQWVVSDGQLREELRSSVAGIVSPAYSNF 595

Query: 601 IGRYQSLPEL-AKRTDRYLKYTAEDMEARITELFE 626
           I R +  PE+  +R + ++ YT ED+E  I  LF+
Sbjct: 601 IRRLKESPEINGRRGEPFIPYTVEDVEFIIKRLFK 595

BLAST of Lsi02G012500 vs. TAIR 10
Match: AT5G50380.1 (exocyst subunit exo70 family protein F1 )

HSP 1 Score: 370.5 bits (950), Expect = 2.6e-102
Identity = 229/666 (34.38%), Postives = 379/666 (56.91%), Query Frame = 0

Query: 6   EEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFSLDKLS------DKADELDPRAPA 65
           E+++LA A+ I K++   + + +D+L IFS+FD R S  K +      ++ D L  R  A
Sbjct: 23  EDRVLATAQQIMKSLNTPKEVREDMLLIFSSFDNRLSNIKTAMTNQEENQNDALVARLEA 82

Query: 66  A---LQR------SLKSLDRRISQYLAADHPIWADSA--DSSAFLDAIDELMAIIRDWAP 125
           A   + R      S +        Y ++   +  D +  +++ FL A+DE+++++ D + 
Sbjct: 83  AESVIHRWDGGNDSSRHSSSSSGNYRSSSFSLSFDESPEEATEFLSAVDEIISLLEDLSS 142

Query: 126 MARDKSVAAYLARADDLMQQAMFRVDQEFRSLMDRG----------GESFELTRHFRNGE 185
             +   V     RAD  +Q AM +++ EFR ++ R           G    ++  F +G+
Sbjct: 143 ENKPDMV----DRADSALQMAMSQLEDEFRRILIRNTVPLDAERLYGSMRRVSLSFADGD 202

Query: 186 STGDF-CFDSEEDEDGAEGILGDGNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRM 245
              DF  F    D DG+    G    L       +   ++ +D +    + DL EIA+RM
Sbjct: 203 VVEDFENFGLVADGDGS----GSRRRLFHERGGSI-GCDLWVDLINPTAVEDLKEIAERM 262

Query: 246 VAAGFEKECSHAYSSCRREFLEESLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVS 305
           + AG+EKEC   YSS RR+ L++ L  LG++KLSIEEVQK+ W  ++E++++W++A+ ++
Sbjct: 263 IRAGYEKECVQVYSSVRRDALDDCLMILGVEKLSIEEVQKIDWKSMDEKMKKWIQAVKIT 322

Query: 306 LRILFPSERRLCERVFVGLSSTADLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILD 365
           +R+L   E+++C+ +F    S+ ++ F E  +   +QLLNF +AVAIG R+ E+LF+ILD
Sbjct: 323 VRVLLVGEKKICDEIFSSSESSKEVCFNETTKSCVMQLLNFGEAVAIGRRSSEKLFRILD 382

Query: 366 MFETLRDLMPEFDSIFSDQYCLLLRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVP 425
           M++ L +++   + + +D +   + NE   + + LG   +G F+E EN +R + +K P  
Sbjct: 383 MYDALANVLQTLEVMVTDCF---VCNETKGVLEALGDAARGTFVEFENNVRNETSKRPTT 442

Query: 426 GGGLHPITRYVMNYLRAACKSRQTLEQVFE--EAALPSKDYTKLEDRAAASSSLSVQMDW 485
            G +HP+ RYVMNY++       TL  + E  E++  S D     D     S L+ ++  
Sbjct: 443 NGEVHPMIRYVMNYMKLIVDYAVTLNSLLESNESSGVSGD-----DSTEEMSPLAKRILG 502

Query: 486 IMELLESNLEAKSKIYKDLSLSSVFLMNNGRYIVQKVKDSELGSVLGDDWIRKHSVKNRQ 545
           ++  LESNLE KSK+Y+D  L  VF+MNN  YIVQKVKDSELG +LGDDW+RK   + RQ
Sbjct: 503 LITSLESNLEDKSKLYEDGGLQHVFMMNNIYYIVQKVKDSELGKLLGDDWVRKRRGQIRQ 562

Query: 546 YLGNYLKSSWSKVVGALKMD---------------SGTLAPAVMKEKLQSFNMQFEEICQ 605
           Y   YL++SWS+V+ AL+ +               S   +   +KE+ + FN  FEE+ +
Sbjct: 563 YATGYLRASWSRVLSALRDESMGGSSSGSPSYGQRSNNSSKMALKERFRGFNASFEELYR 622

Query: 606 TQSTWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLPELAKRTDRYLKYTAEDMEARI 627
            Q+ W + + QL+EE RIS+++ ++PAY+ F GR +S  E  +   +Y+KYT +D+E+ +
Sbjct: 623 LQTAWKVPDPQLREELRISISEKVIPAYRAFFGRNRSQLEGGRHAGKYIKYTPDDLESYL 671

BLAST of Lsi02G012500 vs. TAIR 10
Match: AT5G13150.1 (exocyst subunit exo70 family protein C1 )

HSP 1 Score: 349.7 bits (896), Expect = 4.8e-96
Identity = 207/603 (34.33%), Postives = 339/603 (56.22%), Query Frame = 0

Query: 43  LDKLSDKADELDPRAPAALQRSLKSLDRRISQYLAADHPIWADSADSSAFLDAIDELMAI 102
           +  LS + D L   +P A++   +++D  +S+  ++   +  D  + S F+DA++ +   
Sbjct: 60  IQTLSSEKDPLGEISP-AVESFPEAVDSLVSKMESSG--LGRDETEDSVFIDAVNRISKS 119

Query: 103 IRDWAPMARDKS-VAAYLARADDLMQQAMFRVDQEFRSLMDRGGESFELTRHFRNGESTG 162
           +     +  D + V+++L RA  +  +A+  +D+EFR L+DR  E  +   +  N     
Sbjct: 120 VMRLRELKLDSTPVSSWLNRASSVQHRAVSLLDEEFRHLLDRSREEEKKNNNNNNHHDGS 179

Query: 163 DFCFDSEEDEDGAEGILGDGNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAG 222
           +   ++    D    +L D    +    +   D++         +IS L +IA  M++AG
Sbjct: 180 NSDHNNSSTNDSDRCVLQDHEEAE---EESFHDFS-------PESISTLKKIAGAMISAG 239

Query: 223 FEKECSHAYSSCRREFLEESLSRLGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRIL 282
           +E EC  +Y   RR   +E L+ +G + +++E+VQ++ W  LE EI  W+  +     +L
Sbjct: 240 YEAECCMSYEMSRRHAFKEELTEVGFEGINVEDVQRIGWESLEGEIASWISIVRRCSTVL 299

Query: 283 FPSERRLCERVFVGL--SSTADLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMF 342
           FP E  LC  VF     SS     F  +    TI+ L+F+ AV +  R+ E+LFK LDM+
Sbjct: 300 FPGELSLCNAVFPDQDHSSVRKRLFTGLVSAVTIRFLDFSGAVVLTKRSSEKLFKFLDMY 359

Query: 343 ETLRDLMPEFDSIFSDQYCLLLRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGG 402
           ETLRDL+P  +   SD     L  E      RLG     IF ELE  I+ D  +TPVP G
Sbjct: 360 ETLRDLIPAVEQSDSD-----LIQEIKLAQTRLGEAAVTIFGELEKSIKSDNGRTPVPSG 419

Query: 403 GLHPITRYVMNYLRAACKSRQTLEQVFE-----------EAALPSKDYTKLEDRAAASSS 462
            +HP+TRY MNYL+ AC+ ++TL+QVF+           E     +   + +D     S+
Sbjct: 420 AVHPLTRYTMNYLKYACEYKETLDQVFQHYEANQTDNKPEPETKPRQQQREDDEEYKVSA 479

Query: 463 LSVQMDWIMELLESNLEAKSKIYKDLSLSSVFLMNNGRYIVQKVKDS-ELGSVLGDDWIR 522
            + QM  +MELL++NLE KS++Y+D SL  +FLMNNGRYI+QK+K S E+  ++G  W R
Sbjct: 480 FARQMIRVMELLDANLEIKSRLYRDPSLRFIFLMNNGRYILQKIKGSIEIRDLMGQSWTR 539

Query: 523 KHSVKNRQYLGNYLKSSWSKVVGALKMD----SGTLAPAVMKEKLQSFNMQFEEICQTQS 582
           K S + RQY  +Y + +W KV+  +  +    +G ++  V+KE+ + FN  F+EI +TQS
Sbjct: 540 KRSTELRQYHKSYQRETWGKVLQCMNQEGLQVNGKVSKPVLKERFKIFNAMFDEIHKTQS 599

Query: 583 TWVIFEHQLKEEARISVAKILLPAYQKFIGRYQSLPELAKRTDRYLKYTAEDMEARITEL 627
           TW++ + Q++ E R+S++ +++PAY+ F GRY+   +  K+TD+Y+KY  ED+E+ I +L
Sbjct: 600 TWIVSDEQMQSELRVSISSLVIPAYRSFFGRYKQHLDSGKQTDKYVKYQPEDIESFIDDL 644

BLAST of Lsi02G012500 vs. TAIR 10
Match: AT5G13990.1 (exocyst subunit exo70 family protein C2 )

HSP 1 Score: 349.0 bits (894), Expect = 8.2e-96
Identity = 227/649 (34.98%), Postives = 357/649 (55.01%), Query Frame = 0

Query: 2   VDNGEEKLLAMARHIAKTMGRNESMADDILQIFSNFDARFS--LDKLSDKADELDPRAPA 61
           V  GEE     A  I +T+   ES+++++ Q             D   +K ++   + P 
Sbjct: 64  VSQGEE-----APEIRQTL---ESLSEELDQFLPTLSLHMEEHKDSTEEKGEDGYFQIPQ 123

Query: 62  ALQRSLKSLDRRISQYLAAD-HPIW-ADSADSSAFLDAIDELMAIIRDWAPMARDKSV-- 121
            + + L   + ++S+Y + +   +W  D  + S+ L+A+D +  ++     +   KS   
Sbjct: 124 FVGKFLDLFEEKLSKYDSGEPKTVWYQDPEEVSSLLEAVDRVSKLM---GLLLNTKSCLD 183

Query: 122 --AAYLARADDLMQQAMFRVDQEFRSLMDRG--GESFELTRHFRN-GESTGDFCFDSEED 181
              + +  A  + Q+AM  ++ EFR +++     ES  +T    +   ST D     ++D
Sbjct: 184 HHESLINHAGSIQQRAMAFLEDEFRIILEESVTKESVVVTDDSNSQRRSTAD-----QQD 243

Query: 182 EDGAEGILGDGNALQIPVAQPVTDYNIVIDALPSGTISDLHEIAKRMVAAGFEKECSHAY 241
                 +  D + + +P      D  I     P   +  L +IA++M A G+  EC   Y
Sbjct: 244 HQNDVVVSQDHDQMLVP---ECGDQEIEYPGYPEDVVVVLRKIAEKMKAGGYGWECREVY 303

Query: 242 SSCRREFLEESLSR-LGLQKLSIEEVQKMQWHDLEEEIERWMKAITVSLRILFPSERRLC 301
              RR  L  +L +    +K+SI+EVQKM W  LE EI  W K       + FP E +L 
Sbjct: 304 LVGRRNILMRTLKQDCEFEKVSIDEVQKMSWDTLEREIPIWNKTFKDCSSLFFPGELKLA 363

Query: 302 ERVFVGLSSTADLSFMEVCRGSTIQLLNFADAVAIGSRAPERLFKILDMFETLRDLMPEF 361
           ER+F G        F  V  G  IQ L FA+AVA+  R+ E+LFKILD++ETLRD  P  
Sbjct: 364 ERIFPGDEGNL---FCIVTHGLAIQFLGFAEAVAMTRRSTEKLFKILDIYETLRDSFPAM 423

Query: 362 DSIFSDQYCLLLRNEAITIWKRLGGTIKGIFMELENLIRRDPAKTPVPGGGLHPITRYVM 421
           + +F ++    LRNE  +   RLG T   IF +LE+ I+ D +KTPVPGG +HP+TRY M
Sbjct: 424 EELFPEELRSELRNEVTSARSRLGETAIHIFCDLEHSIKSDSSKTPVPGGAVHPLTRYTM 483

Query: 422 NYLRAACKSRQTLEQVFEEAALPSKDYTKLEDRAAASSSLSVQMDWIMELLESNLEAKSK 481
           NYL+ +C+ + TLEQVF+  +   ++  + E   + +S+ + Q+  IMELL+ NLE KSK
Sbjct: 484 NYLKYSCEYKDTLEQVFKSHSKMERE--EEEPVESGNSAFASQLMRIMELLDGNLETKSK 543

Query: 482 IYKDLSLSSVFLMNNGRYIVQKVKDS-ELGSVLGDDWIRKHSVKNRQYLGNYLKSSWSKV 541
            YKD+ LS +F+MNNGRYIVQK+K S E+  V+GD W R+ S + R Y  NY + +W K+
Sbjct: 544 QYKDIPLSCIFMMNNGRYIVQKIKGSAEIHEVMGDTWCRRRSSELRNYHKNYQRETWGKL 603

Query: 542 VGAL----KMDSGTLAPAVMKEKLQSFNMQFEEICQTQSTWVIFEHQLKEEARISVAKIL 601
           +G L     M +G +    +KE+ +SFN  F+EI +TQ+TWV+ + QL+ E R+S+  ++
Sbjct: 604 LGFLGHEGLMHNGKIVKPNLKERFKSFNATFDEIHKTQTTWVVNDEQLQSELRVSITAVM 663

Query: 602 LPAYQKFIGRYQSLPELAKRTDRYLKYTAEDMEARITELFEGGGGGSGS 634
           +PAY+ F+ R+    +  ++T++Y+KY  ED+E  I +LFEG    S +
Sbjct: 664 IPAYRAFMARFGQYLDPGRQTEKYVKYQPEDIEDLIDQLFEGNTSSSST 688

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FGH91.9e-24367.51Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=... [more]
Q9LMJ44.7e-17352.13Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=... [more]
Q9LZD39.7e-9432.92Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=... [more]
F4KG582.0e-7032.69Exocyst complex component EXO70A3 OS=Arabidopsis thaliana OX=3702 GN=EXO70A3 PE=... [more]
Q8VY275.2e-6329.26Exocyst complex component EXO70H1 OS=Arabidopsis thaliana OX=3702 GN=EXO70H1 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0LVL70.0e+0094.81Exocyst subunit Exo70 family protein OS=Cucumis sativus OX=3659 GN=Csa_1G533450 ... [more]
A0A5A7V2N90.0e+0094.03Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3C4U40.0e+0094.03Exocyst subunit Exo70 family protein OS=Cucumis melo OX=3656 GN=LOC103497050 PE=... [more]
A0A6J1HK700.0e+0091.54Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC1114648... [more]
A0A6J1I2X00.0e+0091.51Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111469428... [more]
Match NameE-valueIdentityDescription
XP_038894987.10.0e+0096.38exocyst complex component EXO70B1-like [Benincasa hispida][more]
XP_004154337.10.0e+0094.81exocyst complex component EXO70B1 [Cucumis sativus] >KGN65838.1 hypothetical pro... [more]
XP_008457332.10.0e+0094.03PREDICTED: exocyst complex component EXO70B1-like [Cucumis melo] >KAA0060021.1 e... [more]
XP_023519988.10.0e+0091.97exocyst complex component EXO70B1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023519989.10.0e+0091.97exocyst complex component EXO70B1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G58430.11.4e-24467.51exocyst subunit exo70 family protein B1 [more]
AT1G07000.13.4e-17452.13exocyst subunit exo70 family protein B2 [more]
AT5G50380.12.6e-10234.38exocyst subunit exo70 family protein F1 [more]
AT5G13150.14.8e-9634.33exocyst subunit exo70 family protein C1 [more]
AT5G13990.18.2e-9634.98exocyst subunit exo70 family protein C2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.1280.170Exocyst complex component Exo70coord: 82..629
e-value: 4.7E-179
score: 598.5
NoneNo IPR availablePANTHERPTHR12542:SF96EXOCYST COMPLEX COMPONENT EXO70B1coord: 29..625
IPR004140Exocyst complex component Exo70PFAMPF03081Exo70coord: 268..621
e-value: 4.2E-113
score: 378.2
IPR004140Exocyst complex component Exo70PANTHERPTHR12542EXOCYST COMPLEX PROTEIN EXO70coord: 29..625
IPR016159Cullin repeat-like-containing domain superfamilySUPERFAMILY74788Cullin repeat-likecoord: 83..625

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G012500.1Lsi02G012500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052542 defense response by callose deposition
biological_process GO:0042742 defense response to bacterium
biological_process GO:0050832 defense response to fungus
biological_process GO:0006887 exocytosis
biological_process GO:0006955 immune response
biological_process GO:0009789 positive regulation of abscisic acid-activated signaling pathway
biological_process GO:0015031 protein transport
biological_process GO:0090333 regulation of stomatal closure
biological_process GO:0009414 response to water deprivation
cellular_component GO:0005829 cytosol
cellular_component GO:0000145 exocyst
cellular_component GO:0070062 extracellular exosome