Lsi02G012450 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G012450
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description[Acyl-carrier-protein] S-malonyltransferase
Locationchr02: 16333751 .. 16337524 (-)
RNA-Seq ExpressionLsi02G012450
SyntenyLsi02G012450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGGATATAGGCAAAAGTAGAAAGAAGGTTTTTATACTAACAAGGCAGTTGAACGGTTTTTATTGGTTTTTGCTGCCATCTTCCCAACGTCTCTACCCTTTGGGAACTCCGCAAAAACCTTGGCATTTCCAGTGACCTATAAAACCTCCATTTCTTCTTACATGAAATCTGCTTCTCTTTATTATCCTCCCAAACCTTTCCTCTTCACATTCCCAATTTGATTTCAATCTCCCTCCTCTTCTGCAATCTCCACCGCCGTCATGTCCTCTTCTCTCGCTCTTCCTTCCACAATTTCCCTCAAGAAGTTGCCTCTCAATGCATCCACCTCTTTCAAGAGCTCCACCTCTTTCCTTCCCTTAAGAAATGGATTCCGAAGCTCTACTTCTGCAACCGTCTCTCGATCTAGGGTTTTCATGAGCGCTTCTGTTGGATCGCAGACTTTGGTCGATGATTCCTTGTTCTTGGATTACAAACCCACTTCTGCCTTTCTCTTTCCTGGTCAGGTGAGTTCGTACTGGATTTGGGTTCTTCTTTTTTTGTGATTTGGGTTTGCTTACACTATGCACTTGAATTAGTTTCTGAGGAGAAATTAATTGAAGCGAACGTTTTCAGTAGTGAAAAGTGGTTGAGGATTTCGTAATTTCTGATACTCACATTGACATTGAGGAGGCTAAATAAGGATTTGCCTTAACAGTTTCTTGCTTTTTGATGGGAAAAGTAGATTCGTGTTTGTCTGTTTGTTTTCTTCTTTTTGTGGGTCGGCGTGTGCAAAACTTGTTATTCATCTTTCATAAAAGAAACACGTCATGAGCGATGATAGAAACAGAAGACAGTCACTATTCTGCATCGATGAGAATTCTAAAAAAATGGCATTGCCTATCTTTTTCTTTTACTTTAACTAGTCATCTTTAATTTTCATGAGAGTTGAAGGCTATGGAAGACGAATGTGCAAAAAGCTAGTTCTCCTTTCCGTATCTCAGGATATGGAGTTCAGTTCTTTGAAAGCAATATCCAAGGAGTTCGTCAGTGTCCTGCATTGTACAATCATAGGCCCATAGCAAGAGAAACAAGTATTCTATCTTCTATGGTGACTCTGCTGAGTCATGTTTTATAAAGGTTTCTTTTGCTGAATGTTGTTCAGGTGCCATGAATGCAATGGCTTCGATCTCGATCGAGGAGTAGAAGAAGCAGTTTATTGTCTTAGATTTACCTCATATTTCCCATATTTTATAAAAACCTTGCATATATCATGAAGCTGGGTATAGGACAGTAGTTATGATTTTCTTTTTCTTTTTTTCACTCTGAGTCAGGGTGCACAAGCTGTGGGAATGGGCAAAGAATCTCAAAGTGTTCCTGCCGCAGCTGACTTGTTCAAGAGAGCAAATGATATTTTAGGGTACGTCCCCTATTGATATTCCTCCATCTTGCATTAGTCCTTGCATTGGCTTGTTCAAGGTCAACCATACATAGAGAGTCGACACTCCTCTCAGATGACCAATTAATGGAATGATTGCCCTTGTGATGTAATGTTATTTAGTCTTGGTTTGCAGGTTTGATCTTCTCGATGTCTGCACTAATGGGCCAAAGGAGAAACTAGATTCAACTGTTATAAGTCAGGTGCTTTATCTTGAACTATTAAGTCTTCTCTGAGATGGTTTTTTATTTACTTATAGTCAGAGGATGGCAAGGCAAGCCACTATTCAATTCAATGTGTCTATGCTTACGCCTGTATATAAATGTCGCATTTTGCATATCTATTATGTGTGAATGTTTAGTTGACATTTTATCACTGTTCAAGGTTTTGAACCTGCTGAATTGATTGTGCAATTGCAGCCAGCTATCTATGTCACTAGTCTAGCTGCAGTTGAGCTACTTCGTGCACGTGATGGAGGCCAACAAATAATTGATTCTGTTGATGTCACTTGTGGTCTAAGCTTGGGAGAGTATACTGCATTAGCATTTGCAGGGGCTTTCAGGTGTCTAATCCGACTGACTGTAATCCCAATGTGTACCCGCTTGCATACATAGATTATTAGTTCAGTAAATAGCATCGAATAAGTTTAATGTTCTAATCTAATGCCATCTATCTTAAATCTTTATATGCACGTGATTATGTTTTATGTTTTCAAATTGACATGGTTGCTAATGGTTTTGGTTACTCTTTTAGCTTTGAGGATGGACTCAGGCTGGTCAAACTGAGGGGGGAGGCAATGCAGGTAAACATAAATAGTTGGGTAAAGGATGTTTCGATTTCATCTTGCAGCCTGCATGTTGTTGTAGTCACTGAAACCAACTTCTCCATGTAACTTCCTTCAGGCAGCTGCTGATGGTGCTAAAAGTGCCATGGTCAGTGTCATAGGGCTAGACTCAGAGAAGGTTCAACAGCTGTGTGATGCAGCTAATCAAGAAGTTGATGGAGCCGATAAAGTTCAGATAGCAAATTTCCTATGTCCTGTAAGGTTCTTTTAAATACCCTTCAGCTCAATAAGCTCTACGTTTTAAATTTCATTAGCATAGTTAGATTATCATTCTCATGCAATGCATTGCTACCGTCACAGGGAAATTATGCTGTATCAGGAGGTCTTAAAGGAGTAGAAGTAGTAGAAGCCAAGGCAAAGTCGTTCAAAGCACGAATGACGGTATTTATCCTATGCATACTATCTGTAGTGGAACTTGAATTTTGAAGTTAAATCTTTCAGTTTCGTTAGAACTCACATGGCAAGGCTGCACCATGGCACTGCAGGTACGTCTAGCTGTGGCAGGTGCATTCCACACTAGTTTCATGGAACCTGCTGTTTCAAGATTAGAAGCTGCACTTGCAGCAACAGAGATCAGAACTCCCAGAATAGCAGTCATCTCCAATGTCGATGCACAGCCACATGCAGATCCTAACACAATCAAGAAAATTTTGGCACGCCAGGTAAAATTAACTCATAACATTCAGAATGAAGACTACATATAGCATCTTGCATAAAACGGACCGGCCTATTAGTTTTCACCATTAAGTAGTCGGACGATAAACACATGAATCACTTTGTTTAGGGGTCAGGGTCAGCGGAGTGTTTCTTCTGTATGGAAATGAATGCTGACATTGTTCATTATTCCCATTTCAAAACGACAGGTGACTTCTCCTGTTCAGTGGGAAACAACAGTAAAAACTCTACTCACCAAGGGGCTGAAGATGAGTTATGAATTGGGCCCTGGAAAGGTATGAAACTCTACATCAATTTTAAGTCACCTTTTTTTTTTTTTTTTTCTCCATATTATGTTCAATGTATTTAGGGTTATTCTCACAAATGTTTCCTATTTTGGGTCAACAGGTTATAGCTGGGATTGTCAAGAGAGTGGACAAAAGTGCAGAAATTGAGAATATTGCAGCTTGATGAAGTCTAGATTGAAGCAAATTCACTCATGGCTGCAGAGTTCTTTCTCTATCTGCTCTCTCTCCCCATCATTGCCAACAGCATCAAACATAAATTAGTACAATGCTCCTCATTGTTCAGCTAAATATTCGGCCAGATGCTACGATTAGTTCTAGAGCTGCAAGTTTCTAAACAAATGCACGAAAATCATGTATAAGTTACTAGGACAATTGATTATAGTTGGAAGCTTTCCATGAGAAATAATATGCAAGATGAGTTAAAAATTTTAATTGAACCAGTATTTGTTTTAGTTTTCAATCTTGATTTGTTTGAAATCGTTTTGCTTAGTTTGTATTCTCATCTGACTTGTTTTTCGAAGGAAATTAATCCTCTTAAACATGCCTTGAACGA

mRNA sequence

GAAGGATATAGGCAAAAGTAGAAAGAAGGTTTTTATACTAACAAGGCAGTTGAACGGTTTTTATTGGTTTTTGCTGCCATCTTCCCAACGTCTCTACCCTTTGGGAACTCCGCAAAAACCTTGGCATTTCCAGTGACCTATAAAACCTCCATTTCTTCTTACATGAAATCTGCTTCTCTTTATTATCCTCCCAAACCTTTCCTCTTCACATTCCCAATTTGATTTCAATCTCCCTCCTCTTCTGCAATCTCCACCGCCGTCATGTCCTCTTCTCTCGCTCTTCCTTCCACAATTTCCCTCAAGAAGTTGCCTCTCAATGCATCCACCTCTTTCAAGAGCTCCACCTCTTTCCTTCCCTTAAGAAATGGATTCCGAAGCTCTACTTCTGCAACCGTCTCTCGATCTAGGGTTTTCATGAGCGCTTCTGTTGGATCGCAGACTTTGGTCGATGATTCCTTGTTCTTGGATTACAAACCCACTTCTGCCTTTCTCTTTCCTGGTCAGGGTGCACAAGCTGTGGGAATGGGCAAAGAATCTCAAAGTGTTCCTGCCGCAGCTGACTTGTTCAAGAGAGCAAATGATATTTTAGGGTTTGATCTTCTCGATGTCTGCACTAATGGGCCAAAGGAGAAACTAGATTCAACTGTTATAAGTCAGCCAGCTATCTATGTCACTAGTCTAGCTGCAGTTGAGCTACTTCGTGCACGTGATGGAGGCCAACAAATAATTGATTCTGTTGATGTCACTTGTGGTCTAAGCTTGGGAGAGTATACTGCATTAGCATTTGCAGGGGCTTTCAGCTTTGAGGATGGACTCAGGCTGGTCAAACTGAGGGGGGAGGCAATGCAGGCAGCTGCTGATGGTGCTAAAAGTGCCATGGTCAGTGTCATAGGGCTAGACTCAGAGAAGGTTCAACAGCTGTGTGATGCAGCTAATCAAGAAGTTGATGGAGCCGATAAAGTTCAGATAGCAAATTTCCTATGTCCTGGAAATTATGCTGTATCAGGAGGTCTTAAAGGAGTAGAAGTAGTAGAAGCCAAGGCAAAGTCGTTCAAAGCACGAATGACGGTACGTCTAGCTGTGGCAGGTGCATTCCACACTAGTTTCATGGAACCTGCTGTTTCAAGATTAGAAGCTGCACTTGCAGCAACAGAGATCAGAACTCCCAGAATAGCAGTCATCTCCAATGTCGATGCACAGCCACATGCAGATCCTAACACAATCAAGAAAATTTTGGCACGCCAGGTGACTTCTCCTGTTCAGTGGGAAACAACAGTAAAAACTCTACTCACCAAGGGGCTGAAGATGAGTTATGAATTGGGCCCTGGAAAGGTTATAGCTGGGATTGTCAAGAGAGTGGACAAAAGTGCAGAAATTGAGAATATTGCAGCTTGATGAAGTCTAGATTGAAGCAAATTCACTCATGGCTGCAGAGTTCTTTCTCTATCTGCTCTCTCTCCCCATCATTGCCAACAGCATCAAACATAAATTAGTACAATGCTCCTCATTGTTCAGCTAAATATTCGGCCAGATGCTACGATTAGTTCTAGAGCTGCAAGTTTCTAAACAAATGCACGAAAATCATGTATAAGTTACTAGGACAATTGATTATAGTTGGAAGCTTTCCATGAGAAATAATATGCAAGATGAGTTAAAAATTTTAATTGAACCAGTATTTGTTTTAGTTTTCAATCTTGATTTGTTTGAAATCGTTTTGCTTAGTTTGTATTCTCATCTGACTTGTTTTTCGAAGGAAATTAATCCTCTTAAACATGCCTTGAACGA

Coding sequence (CDS)

ATGTCCTCTTCTCTCGCTCTTCCTTCCACAATTTCCCTCAAGAAGTTGCCTCTCAATGCATCCACCTCTTTCAAGAGCTCCACCTCTTTCCTTCCCTTAAGAAATGGATTCCGAAGCTCTACTTCTGCAACCGTCTCTCGATCTAGGGTTTTCATGAGCGCTTCTGTTGGATCGCAGACTTTGGTCGATGATTCCTTGTTCTTGGATTACAAACCCACTTCTGCCTTTCTCTTTCCTGGTCAGGGTGCACAAGCTGTGGGAATGGGCAAAGAATCTCAAAGTGTTCCTGCCGCAGCTGACTTGTTCAAGAGAGCAAATGATATTTTAGGGTTTGATCTTCTCGATGTCTGCACTAATGGGCCAAAGGAGAAACTAGATTCAACTGTTATAAGTCAGCCAGCTATCTATGTCACTAGTCTAGCTGCAGTTGAGCTACTTCGTGCACGTGATGGAGGCCAACAAATAATTGATTCTGTTGATGTCACTTGTGGTCTAAGCTTGGGAGAGTATACTGCATTAGCATTTGCAGGGGCTTTCAGCTTTGAGGATGGACTCAGGCTGGTCAAACTGAGGGGGGAGGCAATGCAGGCAGCTGCTGATGGTGCTAAAAGTGCCATGGTCAGTGTCATAGGGCTAGACTCAGAGAAGGTTCAACAGCTGTGTGATGCAGCTAATCAAGAAGTTGATGGAGCCGATAAAGTTCAGATAGCAAATTTCCTATGTCCTGGAAATTATGCTGTATCAGGAGGTCTTAAAGGAGTAGAAGTAGTAGAAGCCAAGGCAAAGTCGTTCAAAGCACGAATGACGGTACGTCTAGCTGTGGCAGGTGCATTCCACACTAGTTTCATGGAACCTGCTGTTTCAAGATTAGAAGCTGCACTTGCAGCAACAGAGATCAGAACTCCCAGAATAGCAGTCATCTCCAATGTCGATGCACAGCCACATGCAGATCCTAACACAATCAAGAAAATTTTGGCACGCCAGGTGACTTCTCCTGTTCAGTGGGAAACAACAGTAAAAACTCTACTCACCAAGGGGCTGAAGATGAGTTATGAATTGGGCCCTGGAAAGGTTATAGCTGGGATTGTCAAGAGAGTGGACAAAAGTGCAGAAATTGAGAATATTGCAGCTTGA

Protein sequence

MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQTLVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNGPKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFSFEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFLCPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIRTPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIAGIVKRVDKSAEIENIAA
Homology
BLAST of Lsi02G012450 vs. ExPASy Swiss-Prot
Match: Q8R3F5 (Malonyl-CoA-acyl carrier protein transacylase, mitochondrial OS=Mus musculus OX=10090 GN=Mcat PE=1 SV=3)

HSP 1 Score: 209.5 bits (532), Expect = 6.4e-53
Identity = 122/305 (40.00%), Postives = 174/305 (57.05%), Query Frame = 0

Query: 74  SAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNGPKEKLDSTVISQP 133
           S  LFPGQG QAVGMG     +P    L++ A+ +LG+DLL++C  GP+E LD TV  QP
Sbjct: 61  SVLLFPGQGCQAVGMGSGLLHLPRVRQLYEAAHRVLGYDLLELCLRGPQEDLDRTVHCQP 120

Query: 134 AIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFSFEDGLRLVKLRGE 193
           A++V SLAAVE L        +ID+     G S+GE+ AL FAGA  F +GL  VK R E
Sbjct: 121 AVFVASLAAVEKLHHLQ--PAVIDNCVAAAGFSVGEFAALVFAGAMDFSEGLYAVKARAE 180

Query: 194 AMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGA----DKVQIANFLCPGNYAVSG 253
           AMQ A++   S M+SV+G         C  A +            Q++N+L P    +SG
Sbjct: 181 AMQEASEAVPSGMLSVLGQRQSNFSFACLEAQEHCKSLGIENPVCQVSNYLFPDCRVISG 240

Query: 254 GLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIRTPRIAVISN 313
            L+ ++ +   +  +  R T  L V+G FHT  MEPAV  L   L +  I+ P +AV SN
Sbjct: 241 HLEALQFLRRNSAKYHFRRTKMLPVSGGFHTCLMEPAVDPLMKVLGSINIKKPLVAVHSN 300

Query: 314 VDAQPHADPNTIKKILARQVTSPVQWETTVKTLL--TKGLKM--SYELGPGKVIAGIVKR 371
           V  Q +  P  I+K+L +QV SPV+WE T+ ++    KG++   +YE+GPG+ +  I+K 
Sbjct: 301 VSGQKYTHPQHIRKLLGQQVVSPVKWEQTMHSIYERKKGMEFPSTYEVGPGQQLGSILKC 360

BLAST of Lsi02G012450 vs. ExPASy Swiss-Prot
Match: Q8IVS2 (Malonyl-CoA-acyl carrier protein transacylase, mitochondrial OS=Homo sapiens OX=9606 GN=MCAT PE=1 SV=2)

HSP 1 Score: 207.6 bits (527), Expect = 2.5e-52
Identity = 120/305 (39.34%), Postives = 174/305 (57.05%), Query Frame = 0

Query: 74  SAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNGPKEKLDSTVISQP 133
           S  LFPGQG+Q VGMG+   + P   +L+  A  +LG+DLL++  +GP+E LD TV  QP
Sbjct: 63  SVLLFPGQGSQVVGMGRGLLNYPRVRELYAAARRVLGYDLLELSLHGPQETLDRTVHCQP 122

Query: 134 AIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFSFEDGLRLVKLRGE 193
           AI+V SLAAVE L        +I++     G S+GE+ AL FAGA  F +GL  VK+R E
Sbjct: 123 AIFVASLAAVEKLHHLQ--PSVIENCVAAAGFSVGEFAALVFAGAMEFAEGLYAVKIRAE 182

Query: 194 AMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGA----DKVQIANFLCPGNYAVSG 253
           AMQ A++   S M+SV+G    K    C  A +            +++N+L P    +SG
Sbjct: 183 AMQEASEAVPSGMLSVLGQPQSKFNFACLEAREHCKSLGIENPVCEVSNYLFPDCRVISG 242

Query: 254 GLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIRTPRIAVISN 313
             + +  ++  +  F  R T  L V+GAFHT  MEPAV  L  AL A +I+ P ++V SN
Sbjct: 243 HQEALRFLQKNSSKFHFRRTRMLPVSGAFHTRLMEPAVEPLTQALKAVDIKKPLVSVYSN 302

Query: 314 VDAQPHADPNTIKKILARQVTSPVQWETTVKTLLT----KGLKMSYELGPGKVIAGIVKR 371
           V A  +  P  I K+LA+Q+ SPV+WE T+  +      +G   ++E+GPG+ +  I+K 
Sbjct: 303 VHAHRYRHPGHIHKLLAQQLVSPVKWEQTMHAIYERKKGRGFPQTFEVGPGRQLGAILKS 362

BLAST of Lsi02G012450 vs. ExPASy Swiss-Prot
Match: P71019 (Malonyl CoA-acyl carrier protein transacylase OS=Bacillus subtilis (strain 168) OX=224308 GN=fabD PE=3 SV=2)

HSP 1 Score: 204.1 bits (518), Expect = 2.7e-51
Identity = 119/295 (40.34%), Postives = 179/295 (60.68%), Query Frame = 0

Query: 75  AFLFPGQGAQAVGMGKE-SQSVPAAADLFKRANDILGFDLLDVCTNGPKEKLDSTVISQP 134
           AFLFPGQG+Q +GMGKE  + VPAA  LF  A++ L   L  +   G  E+L  T  +QP
Sbjct: 5   AFLFPGQGSQFIGMGKELYEQVPAAKRLFDEADETLETKLSSLIFEGDAEELTLTYNAQP 64

Query: 135 AIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFSFEDGLRLVKLRGE 194
           A+  TS+A +E  + ++ G     + D T G SLGEY+AL  AGA SF+D +  V+ RGE
Sbjct: 65  ALLTTSIAVLE--KFKESG----ITPDFTAGHSLGEYSALVAAGALSFKDAVYTVRKRGE 124

Query: 195 AMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFLCPGNYAVSGGLKG 254
            M  A    + AM +++G+D+E ++Q+ D   +E    + VQ+AN  CPG   +SG  KG
Sbjct: 125 FMNEAVPAGEGAMAAILGMDAEALKQVTDKVTEE---GNLVQLANLNCPGQIVISGTAKG 184

Query: 255 VEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIRTPRIAVISNVDAQ 314
           VE+    AK   A+  + L V+G FH+  M+PA  +L+  L A +I+   + VISNV A 
Sbjct: 185 VELASELAKENGAKRAIPLEVSGPFHSELMKPAAEKLKEVLDACDIKDADVPVISNVSAD 244

Query: 315 PHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIAGIVKRVDK 369
              +   IK+ L  Q+ SPV++E ++  L+ +G+    E+GPGKV++G+VK+V++
Sbjct: 245 VMTEKADIKEKLIEQLYSPVRFEESINKLIAEGVTTFIEIGPGKVLSGLVKKVNR 290

BLAST of Lsi02G012450 vs. ExPASy Swiss-Prot
Match: Q8T3L6 (Probable malonyl-CoA-acyl carrier protein transacylase, mitochondrial OS=Drosophila melanogaster OX=7227 GN=beg PE=2 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 3.3e-49
Identity = 122/311 (39.23%), Postives = 175/311 (56.27%), Query Frame = 0

Query: 68  LDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNGPKEKLDS 127
           +D K TS  LFPGQG Q VGM K+    P A  +F+ AN++L +DLL +C  GP+EKL+ 
Sbjct: 62  IDPKETSVMLFPGQGTQYVGMAKDLLRFPGARRIFELANEVLKYDLLKICLEGPREKLNR 121

Query: 128 TVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFSFEDGLRL 187
           T  +Q A+ V+SLAA+E L  R+   + I++     G SLGE TAL +A A  F+  LRL
Sbjct: 122 TEHAQLAVMVSSLAALEQL--REERPKAIETCVAAAGFSLGEITALVYADALPFDKALRL 181

Query: 188 VKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEV--DGADK--VQIANFLCPG 247
           V++R  AMQAA D A  AM   +      + + C  A Q     G +     IAN++ P 
Sbjct: 182 VQVRATAMQAACDQAAGAMAMTLYGPDTNLGEACARAQQWCLDKGVESPYCGIANYMYPH 241

Query: 248 NYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIRTPR 307
              V+G ++ +E +E  AKSFK R   RLAV+GAFHT  M+ AV     AL    ++ P 
Sbjct: 242 CKVVAGNVEALEFLEQNAKSFKIRRMKRLAVSGAFHTPLMQSAVEPFTKALKTVRLQDPV 301

Query: 308 IAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTK----GLKMSYELGPGKVI 367
           I V SNVD +P+     I   L +Q+  PV+WE T+  +  +        ++E GPGK +
Sbjct: 302 IRVYSNVDGKPYRHAKHILTQLPKQIVRPVKWEQTLHEMYERKQGVDFPRTFECGPGKGL 361

Query: 368 AGIVKRVDKSA 371
             ++++V+  A
Sbjct: 362 VQVLEKVNAKA 370

BLAST of Lsi02G012450 vs. ExPASy Swiss-Prot
Match: P43712 (Malonyl CoA-acyl carrier protein transacylase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=fabD PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.2e-44
Identity = 108/293 (36.86%), Postives = 164/293 (55.97%), Query Frame = 0

Query: 75  AFLFPGQGAQAVGMGKE-SQSVPAAADLFKRANDILGFDLLDVCTNGPKEKLDSTVISQP 134
           A +FPGQG+Q VGM  + +   P   + FK+A+D LG+DL  +   GP E+L+ T  +QP
Sbjct: 5   AMVFPGQGSQTVGMLADLATEYPIVIETFKQASDALGYDLWYLVQQGPAEELNKTWQTQP 64

Query: 135 AIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFSFEDGLRLVKLRGE 194
           A+   S+A   + + +    Q+    +V  G SLGEY+AL  AG   F+D ++LV+LRG+
Sbjct: 65  ALLAASVAIYRVWKEK--FPQL--KPEVMAGHSLGEYSALVCAGVLDFQDAIKLVELRGK 124

Query: 195 AMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFLCPGNYAVSGGLKG 254
            MQ A      AM ++IGLD+E +   C  A +     + V   NF  PG   ++G    
Sbjct: 125 LMQQAVPEGTGAMYAIIGLDNEAIINACKQAEE----GEVVSAVNFNSPGQVVIAGAKAA 184

Query: 255 VEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIRTPRIAVISNVDAQ 314
           VE   A  K   A+  + LAV+   H + M+PA  +L   L   +I TP I+V++NVD +
Sbjct: 185 VERAAALCKEAGAKRALPLAVSVPSHCALMKPAAEQLAVTLENIQINTPTISVLNNVDVK 244

Query: 315 PHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIAGIVKRV 367
              +   I+  L RQ+ SPV+W  TV+ +   G+ +  E+GPGKV+ G+ KR+
Sbjct: 245 AETEGTEIRTALVRQLYSPVRWTETVEKMAQDGVLVLAEVGPGKVLNGLTKRI 289

BLAST of Lsi02G012450 vs. ExPASy TrEMBL
Match: A0A6J1I2Y5 ([Acyl-carrier-protein] S-malonyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111469440 PE=4 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 7.9e-187
Identity = 354/377 (93.90%), Postives = 363/377 (96.29%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQT 60
           M+SSLALPSTISLKKLPLNAST F+SS+SFL  RNG RSST  T+SRSRVFMSASVGSQT
Sbjct: 1   MASSLALPSTISLKKLPLNASTPFRSSSSFLGFRNGSRSSTGVTISRSRVFMSASVGSQT 60

Query: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120
           LVDDSLFLDYKPT AFLFPGQGAQAVGMGKESQSVP AADLFKRANDILGFDLLDVCTNG
Sbjct: 61  LVDDSLFLDYKPTCAFLFPGQGAQAVGMGKESQSVPVAADLFKRANDILGFDLLDVCTNG 120

Query: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180
           PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS
Sbjct: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180

Query: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 240
           FEDGLRLVKLRGEAMQAAADGAKSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIANFL
Sbjct: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANFL 240

Query: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300
           CPGNYAVSGGLKGVEVVE+KAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR
Sbjct: 241 CPGNYAVSGGLKGVEVVESKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300

Query: 301 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 360
           TPRI VISNVDAQPHADP+TIKKILARQVTSPV WETTVKTLL KGLK SYELGPGKVIA
Sbjct: 301 TPRIPVISNVDAQPHADPDTIKKILARQVTSPVLWETTVKTLLNKGLKKSYELGPGKVIA 360

Query: 361 GIVKRVDKSAEIENIAA 378
           GI KRVDKSA+IEN+AA
Sbjct: 361 GIFKRVDKSADIENVAA 377

BLAST of Lsi02G012450 vs. ExPASy TrEMBL
Match: A0A6J1HM70 ([Acyl-carrier-protein] S-malonyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111464852 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.3e-186
Identity = 352/377 (93.37%), Postives = 363/377 (96.29%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQT 60
           M+SSLALPSTISLKKLPLNAST F+SS+SFL  RNG RSST  T+SRSRVFMSASVGSQT
Sbjct: 1   MASSLALPSTISLKKLPLNASTPFRSSSSFLGFRNGSRSSTGVTISRSRVFMSASVGSQT 60

Query: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120
           L+DDSLFLDYKPT AFLFPGQGAQAVGMGKESQSVP AADLFKRANDILGFDLLDVCTNG
Sbjct: 61  LIDDSLFLDYKPTCAFLFPGQGAQAVGMGKESQSVPVAADLFKRANDILGFDLLDVCTNG 120

Query: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180
           PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAF+GAFS
Sbjct: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFSGAFS 180

Query: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 240
           FEDGLRLVKLRGEAMQAAADGAKSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIANFL
Sbjct: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANFL 240

Query: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300
           CPGNYAVSGGLKGVEVVE+KAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR
Sbjct: 241 CPGNYAVSGGLKGVEVVESKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300

Query: 301 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 360
           TPRI VISNVDAQPHADP+TIKKILARQVTSPV WETTVKTLL KGLK SYELGPGKVIA
Sbjct: 301 TPRIPVISNVDAQPHADPDTIKKILARQVTSPVLWETTVKTLLNKGLKKSYELGPGKVIA 360

Query: 361 GIVKRVDKSAEIENIAA 378
           GI KRVDKSA+IEN+AA
Sbjct: 361 GIFKRVDKSADIENVAA 377

BLAST of Lsi02G012450 vs. ExPASy TrEMBL
Match: A0A6J1DMV1 ([Acyl-carrier-protein] S-malonyltransferase OS=Momordica charantia OX=3673 GN=LOC111022022 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.3e-184
Identity = 349/378 (92.33%), Postives = 364/378 (96.30%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATV-SRSRVFMSASVGSQ 60
           M+SSLALPSTISLKKLPLN+S+SF+SS SF+  RNGFRSST+AT+ +RSRVFMSASVGSQ
Sbjct: 1   MASSLALPSTISLKKLPLNSSSSFRSSASFIAFRNGFRSSTAATLAARSRVFMSASVGSQ 60

Query: 61  TLVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTN 120
           TL DDSLFLDYKP  AFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTN
Sbjct: 61  TLADDSLFLDYKPNCAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTN 120

Query: 121 GPKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAF 180
           GPKEKLDST++SQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAF
Sbjct: 121 GPKEKLDSTILSQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAF 180

Query: 181 SFEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANF 240
           SFEDGL+LVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVD ADKVQIANF
Sbjct: 181 SFEDGLKLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDEADKVQIANF 240

Query: 241 LCPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEI 300
           LCPGNYAVSGG+KGVE VEAKAKSFKARMTVRLAV GAFHTSFMEPAVSRLEAALAATEI
Sbjct: 241 LCPGNYAVSGGIKGVEAVEAKAKSFKARMTVRLAVTGAFHTSFMEPAVSRLEAALAATEI 300

Query: 301 RTPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVI 360
           +TPRI VISNVDAQPHADP+ IKKILARQVTSPVQWETTVKTLLTKGLK SYELGPGKVI
Sbjct: 301 KTPRIPVISNVDAQPHADPDAIKKILARQVTSPVQWETTVKTLLTKGLKKSYELGPGKVI 360

Query: 361 AGIVKRVDKSAEIENIAA 378
           AGIVKRVDKSA+IENI A
Sbjct: 361 AGIVKRVDKSADIENIGA 378

BLAST of Lsi02G012450 vs. ExPASy TrEMBL
Match: A0A5D3BD87 ([Acyl-carrier-protein] S-malonyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00670 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.6e-182
Identity = 350/377 (92.84%), Postives = 358/377 (94.96%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQT 60
           M+SS ALPSTI LKK PLNASTSF         RNGFR   +A+VSRSRVFMSASVGSQT
Sbjct: 1   MASSFALPSTIPLKKFPLNASTSF---------RNGFR---TASVSRSRVFMSASVGSQT 60

Query: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120
           LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG
Sbjct: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120

Query: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180
           PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS
Sbjct: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180

Query: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 240
           FEDGLRLVKLRGEAMQAAADG KSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIANFL
Sbjct: 181 FEDGLRLVKLRGEAMQAAADGVKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANFL 240

Query: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300
           CPGNYAVSGGLKG+EVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLE+ALAATEIR
Sbjct: 241 CPGNYAVSGGLKGIEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLESALAATEIR 300

Query: 301 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 360
           TP+I VISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLL+KGLK SYELGPGKVIA
Sbjct: 301 TPKIPVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLSKGLKKSYELGPGKVIA 360

Query: 361 GIVKRVDKSAEIENIAA 378
           GIVKRVDKSAEIENIAA
Sbjct: 361 GIVKRVDKSAEIENIAA 365

BLAST of Lsi02G012450 vs. ExPASy TrEMBL
Match: A0A1S3C584 ([Acyl-carrier-protein] S-malonyltransferase OS=Cucumis melo OX=3656 GN=LOC103497045 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.6e-182
Identity = 350/377 (92.84%), Postives = 358/377 (94.96%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQT 60
           M+SS ALPSTI LKK PLNASTSF         RNGFR   +A+VSRSRVFMSASVGSQT
Sbjct: 1   MASSFALPSTIPLKKFPLNASTSF---------RNGFR---TASVSRSRVFMSASVGSQT 60

Query: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120
           LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG
Sbjct: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120

Query: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180
           PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS
Sbjct: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180

Query: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 240
           FEDGLRLVKLRGEAMQAAADG KSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIANFL
Sbjct: 181 FEDGLRLVKLRGEAMQAAADGVKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANFL 240

Query: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300
           CPGNYAVSGGLKG+EVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLE+ALAATEIR
Sbjct: 241 CPGNYAVSGGLKGIEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLESALAATEIR 300

Query: 301 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 360
           TP+I VISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLL+KGLK SYELGPGKVIA
Sbjct: 301 TPKIPVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLSKGLKKSYELGPGKVIA 360

Query: 361 GIVKRVDKSAEIENIAA 378
           GIVKRVDKSAEIENIAA
Sbjct: 361 GIVKRVDKSAEIENIAA 365

BLAST of Lsi02G012450 vs. NCBI nr
Match: XP_022970470.1 (uncharacterized protein LOC111469440 [Cucurbita maxima])

HSP 1 Score: 662.9 bits (1709), Expect = 1.6e-186
Identity = 354/377 (93.90%), Postives = 363/377 (96.29%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQT 60
           M+SSLALPSTISLKKLPLNAST F+SS+SFL  RNG RSST  T+SRSRVFMSASVGSQT
Sbjct: 1   MASSLALPSTISLKKLPLNASTPFRSSSSFLGFRNGSRSSTGVTISRSRVFMSASVGSQT 60

Query: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120
           LVDDSLFLDYKPT AFLFPGQGAQAVGMGKESQSVP AADLFKRANDILGFDLLDVCTNG
Sbjct: 61  LVDDSLFLDYKPTCAFLFPGQGAQAVGMGKESQSVPVAADLFKRANDILGFDLLDVCTNG 120

Query: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180
           PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS
Sbjct: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180

Query: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 240
           FEDGLRLVKLRGEAMQAAADGAKSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIANFL
Sbjct: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANFL 240

Query: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300
           CPGNYAVSGGLKGVEVVE+KAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR
Sbjct: 241 CPGNYAVSGGLKGVEVVESKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300

Query: 301 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 360
           TPRI VISNVDAQPHADP+TIKKILARQVTSPV WETTVKTLL KGLK SYELGPGKVIA
Sbjct: 301 TPRIPVISNVDAQPHADPDTIKKILARQVTSPVLWETTVKTLLNKGLKKSYELGPGKVIA 360

Query: 361 GIVKRVDKSAEIENIAA 378
           GI KRVDKSA+IEN+AA
Sbjct: 361 GIFKRVDKSADIENVAA 377

BLAST of Lsi02G012450 vs. NCBI nr
Match: XP_038893532.1 (malonyl CoA-acyl carrier protein transacylase [Benincasa hispida])

HSP 1 Score: 661.8 bits (1706), Expect = 3.6e-186
Identity = 357/377 (94.69%), Postives = 364/377 (96.55%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQT 60
           M+SSLALPSTISLKKLPL+ASTSF         RNGFRSST+AT+SRSRVFMSASVGSQT
Sbjct: 1   MASSLALPSTISLKKLPLSASTSF---------RNGFRSSTAATISRSRVFMSASVGSQT 60

Query: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120
           LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAA+LFKRANDILGFDLLDVCTNG
Sbjct: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAAELFKRANDILGFDLLDVCTNG 120

Query: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180
           PKEKLDSTVISQPAIYV SLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS
Sbjct: 121 PKEKLDSTVISQPAIYVNSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180

Query: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 240
           FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVD ADKVQIANFL
Sbjct: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDEADKVQIANFL 240

Query: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300
           CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEI+
Sbjct: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIK 300

Query: 301 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 360
           TPRI VISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLK SYELGPGKVIA
Sbjct: 301 TPRIPVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKKSYELGPGKVIA 360

Query: 361 GIVKRVDKSAEIENIAA 378
           GIVKRVDKSA+IENIAA
Sbjct: 361 GIVKRVDKSADIENIAA 368

BLAST of Lsi02G012450 vs. NCBI nr
Match: XP_022964873.1 (uncharacterized protein LOC111464852 isoform X1 [Cucurbita moschata])

HSP 1 Score: 661.4 bits (1705), Expect = 4.7e-186
Identity = 352/377 (93.37%), Postives = 363/377 (96.29%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQT 60
           M+SSLALPSTISLKKLPLNAST F+SS+SFL  RNG RSST  T+SRSRVFMSASVGSQT
Sbjct: 1   MASSLALPSTISLKKLPLNASTPFRSSSSFLGFRNGSRSSTGVTISRSRVFMSASVGSQT 60

Query: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120
           L+DDSLFLDYKPT AFLFPGQGAQAVGMGKESQSVP AADLFKRANDILGFDLLDVCTNG
Sbjct: 61  LIDDSLFLDYKPTCAFLFPGQGAQAVGMGKESQSVPVAADLFKRANDILGFDLLDVCTNG 120

Query: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180
           PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAF+GAFS
Sbjct: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFSGAFS 180

Query: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 240
           FEDGLRLVKLRGEAMQAAADGAKSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIANFL
Sbjct: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANFL 240

Query: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300
           CPGNYAVSGGLKGVEVVE+KAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR
Sbjct: 241 CPGNYAVSGGLKGVEVVESKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300

Query: 301 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 360
           TPRI VISNVDAQPHADP+TIKKILARQVTSPV WETTVKTLL KGLK SYELGPGKVIA
Sbjct: 301 TPRIPVISNVDAQPHADPDTIKKILARQVTSPVLWETTVKTLLNKGLKKSYELGPGKVIA 360

Query: 361 GIVKRVDKSAEIENIAA 378
           GI KRVDKSA+IEN+AA
Sbjct: 361 GIFKRVDKSADIENVAA 377

BLAST of Lsi02G012450 vs. NCBI nr
Match: KAG6583337.1 (Malonyl-CoA-acyl carrier protein transacylase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 661.4 bits (1705), Expect = 4.7e-186
Identity = 352/377 (93.37%), Postives = 363/377 (96.29%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQT 60
           M+SSLALPSTISLKKLPLNAST F+SS+SFL  RNG RSST  T+SRSRVFMSASVGSQT
Sbjct: 1   MASSLALPSTISLKKLPLNASTPFRSSSSFLGFRNGSRSSTGVTISRSRVFMSASVGSQT 60

Query: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120
           L+DDSLFLDYKPT AFLFPGQGAQAVGMGKESQSVP AADLFKRANDILGFDLLDVCTNG
Sbjct: 61  LIDDSLFLDYKPTCAFLFPGQGAQAVGMGKESQSVPVAADLFKRANDILGFDLLDVCTNG 120

Query: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180
           PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS
Sbjct: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180

Query: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 240
           FEDGLRLVKLRGEAMQAAADGAKSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIANFL
Sbjct: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANFL 240

Query: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300
           CPGNYAVSGGLKGVEVVE+KAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR
Sbjct: 241 CPGNYAVSGGLKGVEVVESKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300

Query: 301 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 360
           TPRI VISNVDAQPHADP+TIKKILARQVTSPV WETTVKTLL KGLK SYELGPGKVIA
Sbjct: 301 TPRIPVISNVDAQPHADPDTIKKILARQVTSPVLWETTVKTLLNKGLKKSYELGPGKVIA 360

Query: 361 GIVKRVDKSAEIENIAA 378
           GI KRVDKSA+IEN++A
Sbjct: 361 GIFKRVDKSADIENVSA 377

BLAST of Lsi02G012450 vs. NCBI nr
Match: XP_023520160.1 (uncharacterized protein LOC111783463 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.4 bits (1700), Expect = 1.8e-185
Identity = 352/377 (93.37%), Postives = 363/377 (96.29%), Query Frame = 0

Query: 1   MSSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQT 60
           M+SSLALPSTISLKKLP NASTSF+SS+SFL  RNG RSST  T+SRSRVFMSASVGSQT
Sbjct: 1   MASSLALPSTISLKKLPRNASTSFRSSSSFLGFRNGSRSSTGVTISRSRVFMSASVGSQT 60

Query: 61  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 120
           LVDDSLFLDYKPT AFLFPGQGAQAVGMGKESQSVP AADLFKRANDILGFDLLDVCTNG
Sbjct: 61  LVDDSLFLDYKPTCAFLFPGQGAQAVGMGKESQSVPVAADLFKRANDILGFDLLDVCTNG 120

Query: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180
           PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS
Sbjct: 121 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 180

Query: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 240
           FEDGLRLVKLRGEAMQAAADGAKSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIANFL
Sbjct: 181 FEDGLRLVKLRGEAMQAAADGAKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANFL 240

Query: 241 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300
           CPGNYAVSGGLKGVEVVE+KAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR
Sbjct: 241 CPGNYAVSGGLKGVEVVESKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 300

Query: 301 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 360
           TPRI VISNV+AQPHADP+TI+KILARQVTSPV WETTVKTLL KGLK SYELGPGKVIA
Sbjct: 301 TPRIPVISNVNAQPHADPDTIRKILARQVTSPVLWETTVKTLLNKGLKKSYELGPGKVIA 360

Query: 361 GIVKRVDKSAEIENIAA 378
           GI KRVDKSA+IEN+AA
Sbjct: 361 GIFKRVDKSADIENVAA 377

BLAST of Lsi02G012450 vs. TAIR 10
Match: AT2G30200.1 (catalytics;transferases;[acyl-carrier-protein] S-malonyltransferases;binding )

HSP 1 Score: 570.5 bits (1469), Expect = 1.0e-162
Identity = 313/377 (83.02%), Postives = 334/377 (88.59%), Query Frame = 0

Query: 2   SSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQ-T 61
           SSSL LPS ISL  L  + + SF           GF    +  +SRSR+ MS S GSQ T
Sbjct: 32  SSSLLLPS-ISLNNLSSSKNASF-----------GF---AAKNLSRSRISMSVSAGSQST 91

Query: 62  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 121
            V DSLF DYKPTSAFLFPGQGAQAVGMGKESQSV AA +L+K+ANDILG+DLLD+C NG
Sbjct: 92  TVHDSLFADYKPTSAFLFPGQGAQAVGMGKESQSVGAAGELYKKANDILGYDLLDICVNG 151

Query: 122 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 181
           PKEKLDSTVISQPAIYVTSLAAVELLR R+GG+QII+SVDVTCGLSLGEYTALAFAGAFS
Sbjct: 152 PKEKLDSTVISQPAIYVTSLAAVELLRVREGGEQIINSVDVTCGLSLGEYTALAFAGAFS 211

Query: 182 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 241
           FEDGL+LVKLRGEAMQAAAD AKSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIAN+L
Sbjct: 212 FEDGLKLVKLRGEAMQAAADAAKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANYL 271

Query: 242 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 301
           CPGNYAVSGGLKG+EVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR
Sbjct: 272 CPGNYAVSGGLKGIEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 331

Query: 302 TPRIAVISNVDAQPHADPNTIKKILARQVTSPVQWETTVKTLLTKGLKMSYELGPGKVIA 361
           +PRI VISNVDAQPHADP+TIKKILARQVTSPVQWETTVKTLL+KGLK SYELGPGKVIA
Sbjct: 332 SPRIPVISNVDAQPHADPDTIKKILARQVTSPVQWETTVKTLLSKGLKSSYELGPGKVIA 391

Query: 362 GIVKRVDKSAEIENIAA 378
           GI KRVDKSA  ENI+A
Sbjct: 392 GIFKRVDKSASFENISA 393

BLAST of Lsi02G012450 vs. TAIR 10
Match: AT2G30200.2 (catalytics;transferases;[acyl-carrier-protein] S-malonyltransferases;binding )

HSP 1 Score: 489.6 bits (1259), Expect = 2.3e-138
Identity = 270/329 (82.07%), Postives = 290/329 (88.15%), Query Frame = 0

Query: 2   SSSLALPSTISLKKLPLNASTSFKSSTSFLPLRNGFRSSTSATVSRSRVFMSASVGSQ-T 61
           SSSL LPS ISL  L  + + SF           GF    +  +SRSR+ MS S GSQ T
Sbjct: 32  SSSLLLPS-ISLNNLSSSKNASF-----------GF---AAKNLSRSRISMSVSAGSQST 91

Query: 62  LVDDSLFLDYKPTSAFLFPGQGAQAVGMGKESQSVPAAADLFKRANDILGFDLLDVCTNG 121
            V DSLF DYKPTSAFLFPGQGAQAVGMGKESQSV AA +L+K+ANDILG+DLLD+C NG
Sbjct: 92  TVHDSLFADYKPTSAFLFPGQGAQAVGMGKESQSVGAAGELYKKANDILGYDLLDICVNG 151

Query: 122 PKEKLDSTVISQPAIYVTSLAAVELLRARDGGQQIIDSVDVTCGLSLGEYTALAFAGAFS 181
           PKEKLDSTVISQPAIYVTSLAAVELLR R+GG+QII+SVDVTCGLSLGEYTALAFAGAFS
Sbjct: 152 PKEKLDSTVISQPAIYVTSLAAVELLRVREGGEQIINSVDVTCGLSLGEYTALAFAGAFS 211

Query: 182 FEDGLRLVKLRGEAMQAAADGAKSAMVSVIGLDSEKVQQLCDAANQEVDGADKVQIANFL 241
           FEDGL+LVKLRGEAMQAAAD AKSAMVS+IGLDSEKVQQLCDAANQEVD ADKVQIAN+L
Sbjct: 212 FEDGLKLVKLRGEAMQAAADAAKSAMVSIIGLDSEKVQQLCDAANQEVDEADKVQIANYL 271

Query: 242 CPGNYAVSGGLKGVEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 301
           CPGNYAVSGGLKG+EVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR
Sbjct: 272 CPGNYAVSGGLKGIEVVEAKAKSFKARMTVRLAVAGAFHTSFMEPAVSRLEAALAATEIR 331

Query: 302 TPRIAVISNVDAQPHADPNTIKKILARQV 330
           +PRI VISNVDAQPHADP+TIKKILARQ+
Sbjct: 332 SPRIPVISNVDAQPHADPDTIKKILARQM 345

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8R3F56.4e-5340.00Malonyl-CoA-acyl carrier protein transacylase, mitochondrial OS=Mus musculus OX=... [more]
Q8IVS22.5e-5239.34Malonyl-CoA-acyl carrier protein transacylase, mitochondrial OS=Homo sapiens OX=... [more]
P710192.7e-5140.34Malonyl CoA-acyl carrier protein transacylase OS=Bacillus subtilis (strain 168) ... [more]
Q8T3L63.3e-4939.23Probable malonyl-CoA-acyl carrier protein transacylase, mitochondrial OS=Drosoph... [more]
P437123.2e-4436.86Malonyl CoA-acyl carrier protein transacylase OS=Haemophilus influenzae (strain ... [more]
Match NameE-valueIdentityDescription
A0A6J1I2Y57.9e-18793.90[Acyl-carrier-protein] S-malonyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HM702.3e-18693.37[Acyl-carrier-protein] S-malonyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1DMV11.3e-18492.33[Acyl-carrier-protein] S-malonyltransferase OS=Momordica charantia OX=3673 GN=LO... [more]
A0A5D3BD872.6e-18292.84[Acyl-carrier-protein] S-malonyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C5842.6e-18292.84[Acyl-carrier-protein] S-malonyltransferase OS=Cucumis melo OX=3656 GN=LOC103497... [more]
Match NameE-valueIdentityDescription
XP_022970470.11.6e-18693.90uncharacterized protein LOC111469440 [Cucurbita maxima][more]
XP_038893532.13.6e-18694.69malonyl CoA-acyl carrier protein transacylase [Benincasa hispida][more]
XP_022964873.14.7e-18693.37uncharacterized protein LOC111464852 isoform X1 [Cucurbita moschata][more]
KAG6583337.14.7e-18693.37Malonyl-CoA-acyl carrier protein transacylase, mitochondrial, partial [Cucurbita... [more]
XP_023520160.11.8e-18593.37uncharacterized protein LOC111783463 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G30200.11.0e-16283.02catalytics;transferases;[acyl-carrier-protein] S-malonyltransferases;binding [more]
AT2G30200.22.3e-13882.07catalytics;transferases;[acyl-carrier-protein] S-malonyltransferases;binding [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014043Acyl transferaseSMARTSM00827PKS_ATcoord: 77..376
e-value: 8.0E-18
score: 61.5
IPR014043Acyl transferasePFAMPF00698Acyl_transf_1coord: 76..359
e-value: 6.9E-28
score: 98.1
IPR001227Acyl transferase domain superfamilyGENE3D3.40.366.10coord: 76..360
e-value: 3.4E-94
score: 317.6
IPR004410Malonyl CoA-acyl carrier protein transacylase, FabD-typeTIGRFAMTIGR00128TIGR00128coord: 75..368
e-value: 3.4E-84
score: 280.7
NoneNo IPR availableGENE3D3.30.70.250coord: 202..274
e-value: 3.4E-94
score: 317.6
NoneNo IPR availablePIRSRPIRSR000446-1PIRSR000446-1coord: 74..375
e-value: 1.9E-90
score: 301.3
NoneNo IPR availablePANTHERPTHR47170:SF4BNAA04G17370D PROTEINcoord: 3..377
NoneNo IPR availablePANTHERPTHR47170MALONYL-COA ACP TRANSACYLASE, ACP-BINDINGcoord: 3..377
IPR016036Malonyl-CoA ACP transacylase, ACP-bindingSUPERFAMILY55048Probable ACP-binding domain of malonyl-CoA ACP transacylasecoord: 204..274
IPR016035Acyl transferase/acyl hydrolase/lysophospholipaseSUPERFAMILY52151FabD/lysophospholipase-likecoord: 75..374

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G012450.1Lsi02G012450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004314 [acyl-carrier-protein] S-malonyltransferase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0016740 transferase activity