Lsi02G012250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G012250
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPseudoU_synth_1 domain-containing protein
Locationchr02: 16045340 .. 16052043 (-)
RNA-Seq ExpressionLsi02G012250
SyntenyLsi02G012250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAGATGTTGTTCACAGTTCAAGATTGGATGCCATGATAGTCTCTTTTCTGCGACTCCCGCTTTCTCGATGGACAACCAAAACTTCATCTTCATGCTTTCGTTTCTCCTACCCTGCTTTCATTTCGCGGACCCATCGTCTTAGGCCTACACTTCCGTGCTATTCCACCGATGCCTTGTCCACTCACTGCGCTCCGGACCAGCTCCGGAAATGGGAAGCTTTTCGTAAGAAGAAGGTCGTTTTGCGGATCGGCTATGTTGGGACTGATTATAGAGGTCTCATTTCATATTACTTTCTGTTTATCTTGACGACTCTGTTGTGAATCTTTCACTAGTTCATTAACTCTTGTGTTTTAAAAATTCAGGGCTGCAAATGCAACGCGAGCAACAGTCACATCCAAGTAAGTTTTTCGCGTGCATTTCTTCTTACATCATTAACATTATGTCATGAAAATGCCTAACGGTGGATTGGCAATGCCATCTTCTGAATATTCTGTTGCTGTGTTTGATTTGTATACTTTCACAGTGAATGTGGTGGTTGTTTTCTCAATTACTTACTTAGCCAAATGTAGGGTACAACAACTAATTTAGTTCACGGTGACTAACTGAAACAGTTGAGATTCACTAAACAATTACAAACTTTATGGCTGATTAATTACATCTTAACTAATTAACCACGTAATTACTTAACATTCCCCTAGCCAGCTCAGTGTTGGGGGGAGTGAACTGTGGATCCCAAACAGTAGTTGACTACTGAAGAGAGGGATCTCAACTGATTTGTCCACGTGAACAGTGGACAAATGAATCGGAGGGAGAAAAGATTGGAAATTTACGGTTGCACAACAAAGCTTTGTTGGCTAAATGGCTTTGGCAGTTTGCCCTTGATCTTGAATCCTTGTGGCAGAAGATTATTGAGAGCAAGTACGGCCCCCGTCCCTATGAGTTGGTGGCACAAGGGGGTTGAAGGCACACACCGAAACCTGTGGAGAGATATTTCTTCTCATATCCCTTCCTTTTCCCATCTTGTTTTTTGTGTTATGAGAGATGCTAAGGAAACGTGTTTTTGGGAAGATCATTGGATGGAGGATAGACCCCCCTCTTCTGGTTTCCTCGGTGATATAATTTGTTCTCCTTAAAAAATTGTCGGGTGTCGGATTTTCTGGTTTGGTTTGGTTGGGGAACTCAGTTTCTTTCTCCTTTGGTTTTCGTGGTCTTTGTCCAATAGAGAAATGACGGAGGTAGCCTCTCTTCTTTCCTTAATTGAGGGCTTCGACTTTAAGCTTGGGAGAAAGCACGTTGGTGTGTGGAGTCCTTCCCCCACGGAGGGCTTCTCTTGTAAGTCTTTTTTGTAGGTTTTTACTAGATCCCTCTCCTGTTGTTGAGTCGGTCTTTGATGCCTTATGGAGGATTAAGGTTCCAAAGAAAGTCGAGTTCTTTTCTTGGCAAGTTTTGTGCGGGCGTGTGAACACGATGGATAAACTTTTGAGAAAGATGCCTTCGTTAATGGGCCCTTGGTGTTGCCGGAAGACAGAGGAAAACCTAGATCACCTTCTTTGGGAGTGTCATTATGCTAGATCTGTGTGGAATGACTTCTTTCAGGAGTTTGATTTTGCAATTGCTTGTCAGAGGGATATTCAGTTGATGATCGGGGAGTTCTTCCTACTTTTGCTTTTCAAAGAGAAAGATCGCATTCTTTGGCTTGCGGGGGTGTGTGCTTTATTGTGGGATCTTTGGGGGGAGAGAAACAACAGAGTGTTTCGTGGTGTAGAGAGAGACCCTAGTGAGGTTTGGTCTTTGGTGAGGTTTCATGTTTTCCTTTGGGCTTCAGTTTCAAAGACTTTTTGTAATTACTCCCTATGTAATATTTTACTTAGTTGGAAACCCTTTCTCTAGGTATTTTTTGGGCTTGGTTTTTTTTGTATGCCCCTTGTTCTTTCATTTTTTCTTAATGAAAGTAGTTGCTTCTAAAAAAAAAAATTAATTTTTCTAAACTAGTTTTACAATGATAAACTGCCTTCATTTTTGTTCTTGAAGTTGATCTTTTTACTACTTGACCTTCACTTTGCACCTCATAATGAAAACCTTTTCCAAAGTACTACACAAGATGCGGAAATATTCACTACTTGAGTCCTAATGCTTTCAATCGTTGAGGCGAATAAGCATGAACATCATTTTTTGTTCTTAGCTCCAAAGCAGGAATCTGGATTTTGACTTGACTTGTCAGCATTTTTAACTCCTTGAAGGTGGGTATTGTATCCTCAGTGGTGTGTCCTTCGTAGGAGGAAGGAAGAGGATCTTGACCATTTAGTACTGACAGATTGGATTCCATGTATTGAAGACAATGCAATCTAATATTAATAGGCAACTTTCCAAGGGGAAGTGTTAAAGAAGTTTGCTGTTACATGCTTTGGGAGAACTAAAGAGTGTGGCAGAATAGTTACTGCTAAAGAAGTACATAAAAAGGACATGTATAAGCAACACCAATGCCATTTAAACTTTGTCATCGATCATGAAATTATGCTCTCACCATGTGTCAGTTCCTAACTACAACTATTTCATTACAATCTAATCGCATGATGATCAATAGAAATAATTGAACTTAGTAACCATTTAACTAACCTTGACATCGATATATTATTTTACGTACATTAATCACAAAGGATTTGGCGAGACTTCCATTTATTTTATTTAGTCATTTATTGATTTTTCTCTTATTTAAAATCTCAGTTCTCTCACCTGAGACAATATAACCAACTGAAGAAGGCTATAGATACATTTATCCTGTTTACTTTTCCCGTATAGCGACAAATATGTTATGGACATTGCCCCTTCTTTACACCCCAGAATAGAAAAGCTGACTGCACTGGATAACCACTGCTTATTTTTTCACACTTCTCTAAGTATCCTAGATAAGCATTTCTTTTCCTTTCAATATTTCTTTTACCATTAAACAATATTAAGTGCTTCCGTTGGCTATGATAATAAAGTACTAATAAGCATGGAATATGAATGATACTGTTTCTCTCTCTCTCTTCTTTCTTTTTTCTTCCTTCTGCTGGGAATTACAGCACAGAAATTAATCTCATACAGTTTTGATGTATAGCAATCGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACTGATAAAGGAGTAAATATCTTGAATGTCTTCGTTGTACCTCATTGTATGACTTTTAAAATCTTATTGGCTGTTTTGATTTTTTTTCTCATCTCTTTCATTTTCAAGGTTCATTCCTTGGCTACAACGATAACCTTAAAAATGGAAATCCCTGAAAGTGCTTGGATGGGCGACCCTTGTGGTATTTCGCTTGCTAATTACATCAACTTTTATCTTCCTGATAATATCAAGGTTTTTGGCATTGTACCTTCACAGAGGTGAGTGTTCGAGTTTTTTTCATTGGTGCTTTTAAGTGAGTAAATGAAGTTTTAGCATTAGCAGCATGATACACTTTGACGTATTTACTACAAAGTGGTAACATTACATTAATTTTGTCACTTGTCAAGGAGCTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCTTATCTCCTCCCCGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACTGAAGTTGATTGCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTCAGGTAGGAAATTATGTCCTTCATTTAAATACATGTAGATCAGGTTTCCCCTCCCTTCGCTGCAAGAAACATGATTTGGTTAGTTTTGATATAGTATAGAACTGTTATTTGGGAAGAGAATTGTATGGCGTTGAAGAACAAAGGTGGATTCTTTTTGGATACAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAAAGTTCGAGCCAAGCAATCTCCAGGATATGACCATAAGTCAGGCAATGAGGTATCACCCAGTGATGTTTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAGATGATACTTTTGTTGAAGGAGTCAAGGAACCAGATCAACGTTCTCCAAGCTTAAGTGTTTGTTATGAAAATCCAACTGAAGTATGCAGTAAACTCGGAGATGGATCAGAACTTCCAAAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAAGATTCTTTGCTGTTCTTGTGGGAAGCTGGAAACATCTTTTGGATATGATTTCATTGAGATCTCCATCTGGGGAGAATCATTCATGTTGCATCAAGTTAGTCTTTCTCTAACTTTGTTTTTATGGTACATAATTTGTTCTCATCCATTCTCGTTCCAATTTGTAAGTTTATCTGTATTTACTCCATTTATTCTTAATTTCAGTTGGCAGCTCTATTACTTAGTTTTGAGTTTTCTGTTCTAACGCCTCTGGTAGCTTAGATCTTGCATCTGGCCATGTGGGAGCCAAAACTTGGTTTTATAACTGTTCTCTTGTGTCCACTCGACAATTTTTTTGCAGCATTATTTCACTTTTTAAACTCTTTCCCCTCTTCAGAAATCAATGCTTAGTGACCTAGTGGGAGTTTCCTGTGCTAGTAGTCCTTCAAAAAATTCAGAAATAGCTATGGTAATATGAAAATAAATTAAATTCTAAAATATTACGTACGATTAAATAATTTGATGGCCCCTATGCTTTGGTGGACGGAAGATATGAGTTTATATGGGTTCAAGCTTTTTGAGCTTGACTTGTTCAATATCTACCATCATAACCTTCATTTACTTCCAGAGGTTCACAATTTGTAGGATCAGCATGCGCGGTTAGACATATCTACTTTAATTTTTCAGCAGTTACCTATTGCTATTTGAAATCAATTCTTATGAAATTAAATTCCAAGAGACTGAGAACCATTAAATAATTTGATGGTCCCTGTGCATTGGTGGACAAAAAATACGAGTTGATGGGTTCAAGCTTTATGAGCTTGACTTGTTAAATATCTACCACCATAACCCTTCATTTACTCCCTGAGGTTCATAAATTCATGGGATCAGCTTACACCATTAGACATGTCTAGTTTAATTTTTCTGCACTTAACTATTGCTATTTGAAATCATTTCTTATTATCTGGTTTAGAAGGTGGAAATAGAAACTCTATTGAGACAATTTGATTTGTTTGAAAACCTTTCTCATCTTTGTTAGAGACTAAACTATTCCACTGAGGTCAAAGCCTCAGAAAGAAACCCAAAATCTCATGGACAAACGGTACAAAAAGTGAAAATCTTGATTGGATAGATACTAGAAGTTTAAAATTTGTTTGAAAAGCTTTTGCTTCTCTGTTAGATATTAAACCATTCCATTGAGGTCTAAGCCTCAGGAAGAAACCCAGAATCTTATGGACAAATGGTACAAAGAGGCACTAACTGGTGCAGTATGATTATATCTAACCCTCTTGAGGTAGAGGTTCAAATCTACACGAACGAAAAGGCATTATTGCAGCAGATTTGGACAGAGAGAAACTAGAATCTATTAGTAAGTTTAGGAATTGGAAGGAGATATGGGAGGCCCAAGGTGGAAAGGTAGGGGGCTTCCTTTAGGCAGATTTCTCATTTCCATTCTTTTTGTACCTTTTGAGCTCGTAATATTATTTTTTTCCTATTAAAAAATAACATCAATAACATATCCTGTGAACAATGTAGTTGGCTTCACTAGTAACTAATATTTGGTAGTTAATGTTCTAATTTCAAATCGAAAGGGCCATTCTGATGAGACTTTTATTACATCCTGGGCAGATACGGAAAATGATAGGAACAGCTGTTGCAGTGAAGCGGAAACTACTCCCAAGAGATATTATAGCATTGTCATTGACCAAGTTCTCTAGAATAGTTCTACCCATTGCTCCAGCTGAGGTTTTAGTCCTAAGAGGGAACAGTTTTTCGAACAGAAAACGACCAGGAGTTTCAAAGAGGCCAGACTTGTTGACACTGAACGAATCAGACGACATTGTGAAGTCGGTGAACGAATTCTACAAATCAATGATATTACCCGAGGTAACTAACTTCCTGGACGCAGAAAGACCTCCTTGGAAAGAATGGGTGGAGAAATTGGACGAGTACACAGGCATTCCCAATGAACAATTGGATGAAGTTAGAAATGCTTGGAAAGCATGGAACTACAGGGTACATTGATCATGAAGAATTAAGGCAGCCTTTGAATTGTCCTGATGTGTCTTTTTGTTGGAAGTAGTAAAAAACTTGAGTAATGAAGAGGATGGGAGGCAGTTGAGTTGATGTGGAGGAGTTATTAATGGTACAAAATTATGGGAATTGGGCATTGAATGGATGGTGGTTTGAGCCACCCACCAGCCGCGGGGGGGCGTTTAATAGCGCAGCATCAACTGCAGACAACCGACTCGGCACTCGGCACTCGGCACAACCATAATGGAGTGAACTCGCATTCAATGTAAACGGCTTAAAAGGCGAAATATGTAGGTGAGAGCCTTTGACAGGGATGTACTGATGTTGATTTAACTTGATTTGTGAAGACAATTTGGATCTAAACTTGACTAATTGCCATTCTGAAGCCTTCATGTTGCTTTCTCCTCTTTTAATTTACAATGAAATTCTGTTTATTGTATATTGTTTTCTTTGAGATGCCCA

mRNA sequence

TAAGATGTTGTTCACAGTTCAAGATTGGATGCCATGATAGTCTCTTTTCTGCGACTCCCGCTTTCTCGATGGACAACCAAAACTTCATCTTCATGCTTTCGTTTCTCCTACCCTGCTTTCATTTCGCGGACCCATCGTCTTAGGCCTACACTTCCGTGCTATTCCACCGATGCCTTGTCCACTCACTGCGCTCCGGACCAGCTCCGGAAATGGGAAGCTTTTCGTAAGAAGAAGGTCGTTTTGCGGATCGGCTATGTTGGGACTGATTATAGAGGGCTGCAAATGCAACGCGAGCAACAGTCACATCCAACAATCGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACTGATAAAGGAGTTCATTCCTTGGCTACAACGATAACCTTAAAAATGGAAATCCCTGAAAGTGCTTGGATGGGCGACCCTTGTGGTATTTCGCTTGCTAATTACATCAACTTTTATCTTCCTGATAATATCAAGGTTTTTGGCATTGTACCTTCACAGAGGAGCTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCTTATCTCCTCCCCGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACTGAAGTTGATTGCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTCAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAAAGTTCGAGCCAAGCAATCTCCAGGATATGACCATAAGTCAGGCAATGAGGTATCACCCAGTGATGTTTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAGATGATACTTTTGTTGAAGGAGTCAAGGAACCAGATCAACGTTCTCCAAGCTTAAGTGTTTGTTATGAAAATCCAACTGAAGTATGCAGTAAACTCGGAGATGGATCAGAACTTCCAAAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAAGATTCTTTGCTGTTCTTGTGGGAAGCTGGAAACATCTTTTGGATATGATTTCATTGAGATCTCCATCTGGGGAGAATCATTCATGTTGCATCAAATACGGAAAATGATAGGAACAGCTGTTGCAGTGAAGCGGAAACTACTCCCAAGAGATATTATAGCATTGTCATTGACCAAGTTCTCTAGAATAGTTCTACCCATTGCTCCAGCTGAGGTTTTAGTCCTAAGAGGGAACAGTTTTTCGAACAGAAAACGACCAGGAGTTTCAAAGAGGCCAGACTTGTTGACACTGAACGAATCAGACGACATTGTGAAGTCGGTGAACGAATTCTACAAATCAATGATATTACCCGAGGTAACTAACTTCCTGGACGCAGAAAGACCTCCTTGGAAAGAATGGGTGGAGAAATTGGACGAGTACACAGGCATTCCCAATGAACAATTGGATGAAGTTAGAAATGCTTGGAAAGCATGGAACTACAGGGTACATTGATCATGAAGAATTAAGGCAGCCTTTGAATTGTCCTGATGTGTCTTTTTGTTGGAAGTAGTAAAAAACTTGAGTAATGAAGAGGATGGGAGGCAGTTGAGTTGATGTGGAGGAGTTATTAATGGTACAAAATTATGGGAATTGGGCATTGAATGGATGGTGGTTTGAGCCACCCACCAGCCGCGGGGGGGCGTTTAATAGCGCAGCATCAACTGCAGACAACCGACTCGGCACTCGGCACTCGGCACAACCATAATGGAGTGAACTCGCATTCAATGTAAACGGCTTAAAAGGCGAAATATGTAGGTGAGAGCCTTTGACAGGGATGTACTGATGTTGATTTAACTTGATTTGTGAAGACAATTTGGATCTAAACTTGACTAATTGCCATTCTGAAGCCTTCATGTTGCTTTCTCCTCTTTTAATTTACAATGAAATTCTGTTTATTGTATATTGTTTTCTTTGAGATGCCCA

Coding sequence (CDS)

ATGATAGTCTCTTTTCTGCGACTCCCGCTTTCTCGATGGACAACCAAAACTTCATCTTCATGCTTTCGTTTCTCCTACCCTGCTTTCATTTCGCGGACCCATCGTCTTAGGCCTACACTTCCGTGCTATTCCACCGATGCCTTGTCCACTCACTGCGCTCCGGACCAGCTCCGGAAATGGGAAGCTTTTCGTAAGAAGAAGGTCGTTTTGCGGATCGGCTATGTTGGGACTGATTATAGAGGGCTGCAAATGCAACGCGAGCAACAGTCACATCCAACAATCGAACAAGAATTGGAAGCTGCAATATATAAAGCTGGTGGAATTCGTGAAAGTAACTATGGAAATCTGAACAAAATCTCATGGGCAAGGAGTAGTCGAACTGATAAAGGAGTTCATTCCTTGGCTACAACGATAACCTTAAAAATGGAAATCCCTGAAAGTGCTTGGATGGGCGACCCTTGTGGTATTTCGCTTGCTAATTACATCAACTTTTATCTTCCTGATAATATCAAGGTTTTTGGCATTGTACCTTCACAGAGGAGCTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCTTATCTCCTCCCCGCTAAGCTTATTGGAATTGAAAGTAAACTTAAAACTACTGAAGTTGATTGCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTCAGGGGGAGCACCCTTTTCATAACTATACAATACGTTCCAAGTATAGAACAAAAGTTCGAGCCAAGCAATCTCCAGGATATGACCATAAGTCAGGCAATGAGGTATCACCCAGTGATGTTTCGAACTCTGAGTTTGAGGAAAGTGATGGAGAAGATGATACTTTTGTTGAAGGAGTCAAGGAACCAGATCAACGTTCTCCAAGCTTAAGTGTTTGTTATGAAAATCCAACTGAAGTATGCAGTAAACTCGGAGATGGATCAGAACTTCCAAAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAAGATTCTTTGCTGTTCTTGTGGGAAGCTGGAAACATCTTTTGGATATGATTTCATTGAGATCTCCATCTGGGGAGAATCATTCATGTTGCATCAAATACGGAAAATGATAGGAACAGCTGTTGCAGTGAAGCGGAAACTACTCCCAAGAGATATTATAGCATTGTCATTGACCAAGTTCTCTAGAATAGTTCTACCCATTGCTCCAGCTGAGGTTTTAGTCCTAAGAGGGAACAGTTTTTCGAACAGAAAACGACCAGGAGTTTCAAAGAGGCCAGACTTGTTGACACTGAACGAATCAGACGACATTGTGAAGTCGGTGAACGAATTCTACAAATCAATGATATTACCCGAGGTAACTAACTTCCTGGACGCAGAAAGACCTCCTTGGAAAGAATGGGTGGAGAAATTGGACGAGTACACAGGCATTCCCAATGAACAATTGGATGAAGTTAGAAATGCTTGGAAAGCATGGAACTACAGGGTACATTGA

Protein sequence

MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTLPCYSTDALSTHCAPDQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSFGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEWVEKLDEYTGIPNEQLDEVRNAWKAWNYRVH
Homology
BLAST of Lsi02G012250 vs. ExPASy Swiss-Prot
Match: O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 4.4e-142
Identity = 265/507 (52.27%), Postives = 352/507 (69.43%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTLPCYSTDAL----STHCAPDQ 60
           M VSFLR+P+             F YP   +R  R+ P L  +S+ A     ST  +P  
Sbjct: 1   MAVSFLRIPIPSRV---------FWYP---TRLIRVTPALLRFSSSAAFQPSSTSLSPPS 60

Query: 61  -----LRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRES 120
                  KWE++RKKKVV+RIGYVGTDYRGLQ+QR+  S  TIE ELE AIYKAGGIR+S
Sbjct: 61  SDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDS 120

Query: 121 NYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIK 180
           NYG+L+KI WARSSRTDKGVHSLAT+I+LKMEIPE+AW  DP G  LA  I+ +LP+NI+
Sbjct: 121 NYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIR 180

Query: 181 VFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEH 240
           VF ++PS R FDPR+EC +RKYSYLLP  ++GI++   + E+D HI+DF +IL  F+GE+
Sbjct: 181 VFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGEY 240

Query: 241 PFHNYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQ 300
           PFHNYT RS+YR K   K       K  N   P +  + +  ES+  ++  +E  +E ++
Sbjct: 241 PFHNYTQRSRYRRKSEQK------IKQRNGRPPREPKSIKASESEFREENHIEIGEEEEE 300

Query: 301 RSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLE 360
           +     V  E+   V +   D S++    RA+WL+EPDE D+I  +HFRK+  C  GKLE
Sbjct: 301 K----EVDGESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRSGKLE 360

Query: 361 TSFGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAE 420
            S G+ F+EISIWGESFMLHQIRKMIGTAVAVKR+LLPRDII LSL KF+RIVLP+AP+E
Sbjct: 361 NSLGFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSE 420

Query: 421 VLVLRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWK 480
           VL+LRGNSF  R+ P   +RP +    ES+++ K + EFY+++++P+V+ FLD+E+ PWK
Sbjct: 421 VLILRGNSFEVRRLP---ERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEKSPWK 480

Query: 481 EWVEKLDEYTGIPNEQLDEVRNAWKAW 499
           EWV+ LD   G+ +E+L++VR  W+ W
Sbjct: 481 EWVDHLDRNDGLIDEELEDVRKGWEEW 480

BLAST of Lsi02G012250 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.9e-23
Identity = 122/448 (27.23%), Postives = 196/448 (43.75%), Query Frame = 0

Query: 65  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 124
           K+KV + +GY GT Y G+Q+Q +  +  TIE+++  A+  AG I   N  +L K  + R+
Sbjct: 148 KRKVAVMLGYCGTGYNGMQVQND-PNVKTIEKDIYDAMATAGAISAENAVDLKKSGFQRA 207

Query: 125 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 184
           +RTDKGVH+    I+LKM I       DP    + N IN  LP  I+++GI  + + FD 
Sbjct: 208 ARTDKGVHAAGNVISLKMIIE------DP---EIINKINDLLPKQIRIWGIQRTTKGFDC 267

Query: 185 RKECDVRKYSYLLPA------KLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTI 244
           RK C  R Y YLLP       K   + S+L   +   +   F+D            +  +
Sbjct: 268 RKCCSSRIYEYLLPTFSLLPPKPKSVLSELVKEKKLENPDLFEDDQEGIDWWENVKSKIL 327

Query: 245 RSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKE------PDQR 304
            S    +     +  YD +   +         E EE D  + +F + +K+        +R
Sbjct: 328 ASGITQEQIDSITSSYDEQQQQQQQQQQQQADE-EERDISELSFTKLIKQIKTIENQSRR 387

Query: 305 SPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLET 364
           S  +S           K  +G+          + +P  KD        K +      +E 
Sbjct: 388 SYRISSSRLQHFREVMKQYEGTH---NFHNFTVGKP-FKDTSANRFMIKTIVSDPFVIE- 447

Query: 365 SFGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEV 424
             G ++I I I G+SFMLHQIRKMI  A  V R  LP  II  +  + ++I +P APA  
Sbjct: 448 --GTEWISIKIHGQSFMLHQIRKMIAMAALVVRLSLPCGIIN-NFFQSTKINIPKAPALG 507

Query: 425 LVLRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKE 484
           L+L    F            + +   + D   K +NEF    I  ++    +++   +  
Sbjct: 508 LLLENPVFDGYNIKLTKSDYEPIDFTKFD---KEMNEFKMKYIYDKI-YAEESKENIFYG 567

Query: 485 WVEKLDEYTGIPNEQLDEVRNAWKAWNY 501
           +   +D Y G  NE  + ++N    +++
Sbjct: 568 FFGYIDAYRGNKNEDGEPIQNGASIFDF 572

BLAST of Lsi02G012250 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 3.2e-23
Identity = 115/409 (28.12%), Postives = 185/409 (45.23%), Query Frame = 0

Query: 65  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 124
           K+KV   IGY GT Y G+Q+   Q+   TIE ++  A  KAG I ++N  +  K ++ R+
Sbjct: 84  KRKVACMIGYCGTGYHGMQLNPPQK---TIEGDIFQAFVKAGAISQNNADDPKKSAFMRA 143

Query: 125 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 184
           +RTDKGVH+    I+LKM I +          ++   IN +LP+ ++V+G+  + ++F+ 
Sbjct: 144 ARTDKGVHAAGNVISLKMIIEDE---------NIVEKINSHLPEQLRVWGVSRTNKAFEC 203

Query: 185 RKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSKYRT 244
           RK C  R Y YL+P     +  +  T   +  + D         G  P        KY  
Sbjct: 204 RKLCSSRVYEYLMPTYSF-LNPRPGTVMSEKLLKD---------GTSPDEE---GKKYWE 263

Query: 245 KVRA---KQSPGY---------DHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQR 304
            V A    Q   Y         D   G E    +V+ SE +     D   +E +K  ++R
Sbjct: 264 SVAADLESQGVSYDEWMKRACIDEIKGEET--KEVAESEVKTDSKTDAATLEKIKAVERR 323

Query: 305 SPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLET 364
                  +    E  +K+ +  ++ +G    + +    K     S  R +   +C     
Sbjct: 324 HREE---FRISGERLAKIREILKIYEGTH-NFHNFTLGKAFKDPSAMRTMKSLTCSDPFL 383

Query: 365 SFGYDFIEISIWGESFMLHQIRKMIG-TAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAE 424
             G +++ I I G+SFMLHQIRKMI   A++V+    P+ +I  +  K +RI +P APA 
Sbjct: 384 IDGTEWVSIKIHGQSFMLHQIRKMISMVALSVRCNADPQKLIPQTFEK-ARINIPKAPAL 443

Query: 425 VLVLRGNSFS--NRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPE 459
            L+L    +   N+K  G   R  +   N +D I    ++F    I  E
Sbjct: 444 GLLLERPVYDSYNKKLQGEFGREGVHFDNWNDQIEAFKHKFIYDKIYAE 460

BLAST of Lsi02G012250 vs. ExPASy Swiss-Prot
Match: Q755C8 (tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 4.6e-22
Identity = 109/356 (30.62%), Postives = 161/356 (45.22%), Query Frame = 0

Query: 65  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 124
           K+KV + +GY GT Y G+Q     +   TIE EL  A  KAG I  +N  +L K  + R+
Sbjct: 66  KRKVAVMVGYCGTGYHGMQYNPPNR---TIEAELFEAFVKAGAISRANSTDLKKNGFMRA 125

Query: 125 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 184
           +RTDKGVH+    I+LK+ I + A         + + IN +LP  I+V+GI    ++FD 
Sbjct: 126 ARTDKGVHAGGNVISLKLIIEDPA---------IKDKINEHLPPGIRVWGISRVNKAFDC 185

Query: 185 RKECDVRKYSYLLPA-KLIGIE--SKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSK 244
           RK C  R Y YLLP    IG +  + L  T   C  +       S + +    +      
Sbjct: 186 RKLCGSRWYEYLLPTYSFIGPKPNTYLARTIEQCGEA------ASEKPDRDQESLDFWEA 245

Query: 245 YRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYE 304
           +R  V  K +        N V+P   S  +F+E+ G      + VK+  Q   +    Y 
Sbjct: 246 FRKAVDEKFTQEEQDAIVNYVAP---SKEDFDENSG----LYQKVKQYKQMENAHRRSYR 305

Query: 305 NPTEVCSKLGDGSELPKGVR--ARWLHEPDEKDRIGASHFRKILCCSCGKLETSFGYDFI 364
             +   ++  +  +   G      +    D KD      F K +  S   +      +++
Sbjct: 306 VSSAKLARFREAMKQYLGPHNFHNYTLGKDFKDP-STVRFMKDITVSDPFVIGEMKTEWV 365

Query: 365 EISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 416
            I I G+SFMLHQIRKMI  A  V R     + IA S     +I +P APA  L+L
Sbjct: 366 SIKIHGQSFMLHQIRKMISMATLVARCNCSPERIAQSYGP-QKINIPKAPALGLLL 394

BLAST of Lsi02G012250 vs. ExPASy Swiss-Prot
Match: Q6FV05 (tRNA pseudouridine synthase 1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 5.1e-21
Identity = 119/437 (27.23%), Postives = 195/437 (44.62%), Query Frame = 0

Query: 65  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 124
           K+KV + IGY GT Y G+Q       +PTIE  L  A   AG I ++N  +L K ++ R+
Sbjct: 86  KRKVAVMIGYCGTGYHGMQY---NPPNPTIEAALFKAFVDAGAISKANSNDLKKNNFMRA 145

Query: 125 SRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDP 184
           +RTDKGVH+    I+LKM I       DP    + + IN  LP+ I+++ I    ++FD 
Sbjct: 146 ARTDKGVHAGGNLISLKMIIE------DP---EIMDKINANLPEGIRIWDIERVNKAFDC 205

Query: 185 RKECDVRKYSYLLPA-KLIGIE-SKLKTTEVDCHISDFKDILN-SFQGEHPFHNYTIRSK 244
           RK C  R Y YLLP   LIG +   +   +++   ++   +L+   + +  + N+   +K
Sbjct: 206 RKMCSSRWYEYLLPTYSLIGPKPGTILNNDIESSKTELPGVLDEDLESKEFWENFEAAAK 265

Query: 245 YRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYE 304
                        +       +  D S    EE D +++ + + VK+      +    Y 
Sbjct: 266 ------------KEFTPEEIAAIKDYSPPPREEFDDQEELY-QKVKQWKLLENTHRRQYR 325

Query: 305 NPTEVCSKLGDGSELPKGVRARWLHE----PDEKDRIGASHFRKILCCSCGKLETSFGYD 364
                 +K    + + + + A   H      + KD   A  F K +  S   +      +
Sbjct: 326 ISEMKLNKF--RAAMNQYLGAHNFHNFTLGKEFKDP-SAIRFMKEIKVSDPFVIGDAKAE 385

Query: 365 FIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRG 424
           ++ I I G+SFMLHQIRKMI  A  + R   P + I+ +     +I +P APA  L+L  
Sbjct: 386 WVSIKIHGQSFMLHQIRKMISMATLIARCGTPVERISQAFGP-QKINIPKAPALGLLLEA 445

Query: 425 ---NSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVT-NFLDAERPPWKEW 484
               S++ R      K  D     E  D  K +   Y  +   EV+ N  +A    +  +
Sbjct: 446 PVFESYNKRLEKFGYKPIDFSKYQEKVDAFK-MKHIYDKIYAEEVSDNVFNA----FFSY 488

Query: 485 VEKLDEYTGIPNEQLDE 491
           ++  ++ TG   E+  E
Sbjct: 506 IDSFNKVTGAQTEESAE 488

BLAST of Lsi02G012250 vs. ExPASy TrEMBL
Match: A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 7.1e-260
Identity = 449/509 (88.21%), Postives = 469/509 (92.14%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPT-LPCY-----STDALSTHCAP 60
           M VS LRLPLS W TK+SSSCFRF YP F+ RT RLRPT L C+     STDA ST CAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKW+AFRKKKVVLRIGYVGTDYRGLQMQR Q SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGLQMQRNQLSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLA+YIN YLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLADYINSYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTE+DCHISDFKDILNSF+GEHPFH
Sbjct: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDCHISDFKDILNSFEGEHPFH 240

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRS YRT++RAKQSPGY  KSGN+VSP ++SN EFEESD EDDTFVEGVKEPDQ SP
Sbjct: 241 NYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360
           SLSVC EN TEVCSKL DGSEL KGVRARWLHEPDEKDRIGASHFR+ILCCSCGKLETSF
Sbjct: 301 SLSVC-ENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILCCSCGKLETSF 360

Query: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRD+IALSLTKFSRIVLPIAPAEVLV
Sbjct: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDVIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEWV 480
           LRGNSFS RKRPGV  RPDL+TLNESDDI+KSVNEFY+S ILPEVT FLD E PPWKEWV
Sbjct: 421 LRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSAILPEVTEFLDTESPPWKEWV 480

Query: 481 EKLDEYTGIPNEQLDEVRNAWKAWNYRVH 504
           EKLD+YT IPNE+LDEVR+AWKAW YRVH
Sbjct: 481 EKLDKYTSIPNEELDEVRSAWKAWKYRVH 508

BLAST of Lsi02G012250 vs. ExPASy TrEMBL
Match: A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 7.6e-254
Identity = 435/505 (86.14%), Postives = 461/505 (91.29%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTLPC------YSTDALSTHCAP 60
           M VS LR PLS W TKTS  CFRF YP F+S T RLRP L C       STDALST CAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFV-EGVKEPDQRS 300
           NYTIRSKYRT++RA QSPGY HKSG+ VS SD+SNSEFEESDGE+D FV  G+KEP+Q S
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETS 360
           PSLS C EN  EVCSKLG+GSELP+GVRARWLHEPDE+DRIGASHFRKILCCSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 FGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVL 420
            GYDF+EISIWGESFMLHQIRKM+GTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVL
Sbjct: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420

Query: 421 VLRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEW 480
           VLRGN FSNRKRPG  KRPDLLTLN+SDDIVKSVNEFY+S ILPEVTNFLD ERPPWKEW
Sbjct: 421 VLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480

Query: 481 VEKLDEYTGIPNEQLDEVRNAWKAW 499
           VEKLD+YT IPNEQL+EVRNAWKAW
Sbjct: 481 VEKLDKYTSIPNEQLEEVRNAWKAW 505

BLAST of Lsi02G012250 vs. ExPASy TrEMBL
Match: A0A6J1HM83 (putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111464888 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.5e-251
Identity = 430/504 (85.32%), Postives = 456/504 (90.48%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTLPC------YSTDALSTHCAP 60
           M VS LR PLS W  KTS  CFRF YP F+S T RL P L C       STDALST CAP
Sbjct: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NI+VFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYRT++R  QSPGY HKSG+ VS SD+SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360
           SLS  YEN  EVCSKLG+GSELP+GVRARWLHEPDEKDRIGASHFRKIL CSCGKLETS 
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDF+EISIWGESFMLHQIRKM+GTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEWV 480
           LRGN FSNRKRPG  KRPDLLTLNES DIVKSVNEFY+S ILPEVTNFLD ERPPWKEWV
Sbjct: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480

Query: 481 EKLDEYTGIPNEQLDEVRNAWKAW 499
           EKLD+YT IPNEQL+EVRNAWKAW
Sbjct: 481 EKLDKYTSIPNEQLEEVRNAWKAW 504

BLAST of Lsi02G012250 vs. ExPASy TrEMBL
Match: A0A6J1DMV6 (putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 9.0e-247
Identity = 424/504 (84.13%), Postives = 452/504 (89.68%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTL------PCYSTDALSTHCAP 60
           M VS LR PLS W TKTSS+CFR  Y AF+SR+  LRP L      P  STDA ST CAP
Sbjct: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLR WEAFRKKKVV+RIGY GTDYRGLQMQREQQSHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIPE+AWMGDPCGI LANYINFYLPDNIKVFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKYSYLLPAKLIGIESK  TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYR  +RA+QSPGY H SG EVSPS +SNS FEESD ED+ FVEGVK P+Q +P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360
            LSVC E+P EVCSKLG+GSEL KGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETS 
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360

Query: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDF+EISIWGESFMLHQIRKM+GTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEWV 480
           LRGNSFSN KRPG  KRPDLLTLN+SDDI+KSV+EFY+S ILPE+TNFLD E+ PW EWV
Sbjct: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480

Query: 481 EKLDEYTGIPNEQLDEVRNAWKAW 499
           EKLD+YT IPNEQLDEVRNAWKAW
Sbjct: 481 EKLDKYTSIPNEQLDEVRNAWKAW 503

BLAST of Lsi02G012250 vs. ExPASy TrEMBL
Match: A0A6J1DQ07 (putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 9.0e-247
Identity = 424/504 (84.13%), Postives = 452/504 (89.68%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTL------PCYSTDALSTHCAP 60
           M VS LR PLS W TKTSS+CFR  Y AF+SR+  LRP L      P  STDA ST CAP
Sbjct: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLR WEAFRKKKVV+RIGY GTDYRGLQMQREQQSHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIPE+AWMGDPCGI LANYINFYLPDNIKVFG
Sbjct: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKYSYLLPAKLIGIESK  TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYR  +RA+QSPGY H SG EVSPS +SNS FEESD ED+ FVEGVK P+Q +P
Sbjct: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360
            LSVC E+P EVCSKLG+GSEL KGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETS 
Sbjct: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360

Query: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDF+EISIWGESFMLHQIRKM+GTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEWV 480
           LRGNSFSN KRPG  KRPDLLTLN+SDDI+KSV+EFY+S ILPE+TNFLD E+ PW EWV
Sbjct: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480

Query: 481 EKLDEYTGIPNEQLDEVRNAWKAW 499
           EKLD+YT IPNEQLDEVRNAWKAW
Sbjct: 481 EKLDKYTSIPNEQLDEVRNAWKAW 503

BLAST of Lsi02G012250 vs. NCBI nr
Match: XP_038894774.1 (putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 937.6 bits (2422), Expect = 4.6e-269
Identity = 461/509 (90.57%), Postives = 477/509 (93.71%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTL------PCYSTDALSTHCAP 60
           M VS LRLPLS W TKTSSSCF F YP F+SRTHRL PTL         STDA ST CAP
Sbjct: 1   MAVSSLRLPLSPWITKTSSSCFLFPYPTFVSRTHRLGPTLSGCSASSTASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           +QLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  NQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTI LKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTIALKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQ+SFDPRKECDVRKYSY+LPAKLIGI +KLKTTEVD HISDFKDILNSFQGEHPFH
Sbjct: 181 IVPSQKSFDPRKECDVRKYSYVLPAKLIGIGNKLKTTEVDYHISDFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYRT++RAK+SPGY    G+EVSPSDVSNSEFEESDGEDDTFVEGVKEPDQ SP
Sbjct: 241 NYTIRSKYRTRIRAKRSPGY----GHEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQGSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360
           SLSVCYENPTEVCSKLGDGSELPK VRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF
Sbjct: 301 SLSVCYENPTEVCSKLGDGSELPKDVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360

Query: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDF+EISIWGESFMLHQIRKMIGTA+AVKRKLLPRD+IALSL+KFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMIGTAIAVKRKLLPRDVIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEWV 480
           LRGNSFSNRKRPGV KRPDLLTLNESDDIVKSVNEFY+S ILPEVTNFLD ERPPWKEWV
Sbjct: 421 LRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSAILPEVTNFLDTERPPWKEWV 480

Query: 481 EKLDEYTGIPNEQLDEVRNAWKAWNYRVH 504
           EKLD+YT IP+EQLDEVRNAWKAWNYRVH
Sbjct: 481 EKLDKYTSIPSEQLDEVRNAWKAWNYRVH 505

BLAST of Lsi02G012250 vs. NCBI nr
Match: XP_008457308.1 (PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo])

HSP 1 Score: 906.0 bits (2340), Expect = 1.5e-259
Identity = 449/509 (88.21%), Postives = 469/509 (92.14%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPT-LPCY-----STDALSTHCAP 60
           M VS LRLPLS W TK+SSSCFRF YP F+ RT RLRPT L C+     STDA ST CAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKW+AFRKKKVVLRIGYVGTDYRGLQMQR Q SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGLQMQRNQLSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLA+YIN YLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLADYINSYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTE+DCHISDFKDILNSF+GEHPFH
Sbjct: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDCHISDFKDILNSFEGEHPFH 240

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRS YRT++RAKQSPGY  KSGN+VSP ++SN EFEESD EDDTFVEGVKEPDQ SP
Sbjct: 241 NYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360
           SLSVC EN TEVCSKL DGSEL KGVRARWLHEPDEKDRIGASHFR+ILCCSCGKLETSF
Sbjct: 301 SLSVC-ENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILCCSCGKLETSF 360

Query: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRD+IALSLTKFSRIVLPIAPAEVLV
Sbjct: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDVIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEWV 480
           LRGNSFS RKRPGV  RPDL+TLNESDDI+KSVNEFY+S ILPEVT FLD E PPWKEWV
Sbjct: 421 LRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSAILPEVTEFLDTESPPWKEWV 480

Query: 481 EKLDEYTGIPNEQLDEVRNAWKAWNYRVH 504
           EKLD+YT IPNE+LDEVR+AWKAW YRVH
Sbjct: 481 EKLDKYTSIPNEELDEVRSAWKAWKYRVH 508

BLAST of Lsi02G012250 vs. NCBI nr
Match: XP_022970646.1 (putative tRNA pseudouridine synthase [Cucurbita maxima])

HSP 1 Score: 885.9 bits (2288), Expect = 1.6e-253
Identity = 435/505 (86.14%), Postives = 461/505 (91.29%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTLPC------YSTDALSTHCAP 60
           M VS LR PLS W TKTS  CFRF YP F+S T RLRP L C       STDALST CAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFV-EGVKEPDQRS 300
           NYTIRSKYRT++RA QSPGY HKSG+ VS SD+SNSEFEESDGE+D FV  G+KEP+Q S
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETS 360
           PSLS C EN  EVCSKLG+GSELP+GVRARWLHEPDE+DRIGASHFRKILCCSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 FGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVL 420
            GYDF+EISIWGESFMLHQIRKM+GTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVL
Sbjct: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420

Query: 421 VLRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEW 480
           VLRGN FSNRKRPG  KRPDLLTLN+SDDIVKSVNEFY+S ILPEVTNFLD ERPPWKEW
Sbjct: 421 VLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480

Query: 481 VEKLDEYTGIPNEQLDEVRNAWKAW 499
           VEKLD+YT IPNEQL+EVRNAWKAW
Sbjct: 481 VEKLDKYTSIPNEQLEEVRNAWKAW 505

BLAST of Lsi02G012250 vs. NCBI nr
Match: XP_023520301.1 (putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 880.6 bits (2274), Expect = 6.6e-252
Identity = 433/504 (85.91%), Postives = 456/504 (90.48%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTLPC------YSTDALSTHCAP 60
           M VS LR PLS W TKTS  CFRF YP F+  T RLRP L C       STDALST CAP
Sbjct: 18  MAVSSLRFPLSPWATKTSFPCFRFPYPNFVLWTSRLRPRLLCCSSSSTASTDALSTQCAP 77

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 78  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 137

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           NLNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NIKVFG
Sbjct: 138 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 197

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 198 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 257

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYRT++RA QSPGY HKSG+ VS SD SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 258 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDFSNSEFEESDGENDIFVGGIKEPNQHSP 317

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360
           SLS  YEN  EVCSKLG+GSELP+GVRARWLHEPDEKDRIGA HFRKIL CSCGKLETS 
Sbjct: 318 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGAPHFRKILYCSCGKLETSR 377

Query: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDF+EISIWGESFMLHQIRKM+GTA+AVKRKLLPRDIIALSLTKFSRIV+PIAPAEVLV
Sbjct: 378 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLTKFSRIVMPIAPAEVLV 437

Query: 421 LRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEWV 480
           LRGN FSNRKRPG  KRPDLLTLNES DIVKSVNEFY+S ILPEVTNFLD ERPPWKEWV
Sbjct: 438 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 497

Query: 481 EKLDEYTGIPNEQLDEVRNAWKAW 499
           EKLD+YT IPNEQL+EVRNAWKAW
Sbjct: 498 EKLDKYTSIPNEQLEEVRNAWKAW 521

BLAST of Lsi02G012250 vs. NCBI nr
Match: XP_022964935.1 (putative tRNA pseudouridine synthase [Cucurbita moschata])

HSP 1 Score: 877.1 bits (2265), Expect = 7.3e-251
Identity = 430/504 (85.32%), Postives = 456/504 (90.48%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTLPC------YSTDALSTHCAP 60
           M VS LR PLS W  KTS  CFRF YP F+S T RL P L C       STDALST CAP
Sbjct: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120
           DQLRKWEAFRKKKVV+RIGYVGTDYRGLQMQREQ SHPTIEQELEAAIYKAGGIRESNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180
           +LNKISWARSSRTDKGVHSLATTITLKMEIP+SAWMGDPCGI LANYINFYLP+NI+VFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKY+YLLPAKLIGIESKL TTE+D HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSP 300
           NYTIRSKYRT++R  QSPGY HKSG+ VS SD+SNSEFEESDGE+D FV G+KEP+Q SP
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300

Query: 301 SLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360
           SLS  YEN  EVCSKLG+GSELP+GVRARWLHEPDEKDRIGASHFRKIL CSCGKLETS 
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDF+EISIWGESFMLHQIRKM+GTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWKEWV 480
           LRGN FSNRKRPG  KRPDLLTLNES DIVKSVNEFY+S ILPEVTNFLD ERPPWKEWV
Sbjct: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480

Query: 481 EKLDEYTGIPNEQLDEVRNAWKAW 499
           EKLD+YT IPNEQL+EVRNAWKAW
Sbjct: 481 EKLDKYTSIPNEQLEEVRNAWKAW 504

BLAST of Lsi02G012250 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 506.1 bits (1302), Expect = 3.2e-143
Identity = 265/507 (52.27%), Postives = 352/507 (69.43%), Query Frame = 0

Query: 1   MIVSFLRLPLSRWTTKTSSSCFRFSYPAFISRTHRLRPTLPCYSTDAL----STHCAPDQ 60
           M VSFLR+P+             F YP   +R  R+ P L  +S+ A     ST  +P  
Sbjct: 1   MAVSFLRIPIPSRV---------FWYP---TRLIRVTPALLRFSSSAAFQPSSTSLSPPS 60

Query: 61  -----LRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRES 120
                  KWE++RKKKVV+RIGYVGTDYRGLQ+QR+  S  TIE ELE AIYKAGGIR+S
Sbjct: 61  SDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRDS 120

Query: 121 NYGNLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIK 180
           NYG+L+KI WARSSRTDKGVHSLAT+I+LKMEIPE+AW  DP G  LA  I+ +LP+NI+
Sbjct: 121 NYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENIR 180

Query: 181 VFGIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEH 240
           VF ++PS R FDPR+EC +RKYSYLLP  ++GI++   + E+D HI+DF +IL  F+GE+
Sbjct: 181 VFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGEY 240

Query: 241 PFHNYTIRSKYRTKVRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQ 300
           PFHNYT RS+YR K   K       K  N   P +  + +  ES+  ++  +E  +E ++
Sbjct: 241 PFHNYTQRSRYRRKSEQK------IKQRNGRPPREPKSIKASESEFREENHIEIGEEEEE 300

Query: 301 RSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLE 360
           +     V  E+   V +   D S++    RA+WL+EPDE D+I  +HFRK+  C  GKLE
Sbjct: 301 K----EVDGESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRSGKLE 360

Query: 361 TSFGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAE 420
            S G+ F+EISIWGESFMLHQIRKMIGTAVAVKR+LLPRDII LSL KF+RIVLP+AP+E
Sbjct: 361 NSLGFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSE 420

Query: 421 VLVLRGNSFSNRKRPGVSKRPDLLTLNESDDIVKSVNEFYKSMILPEVTNFLDAERPPWK 480
           VL+LRGNSF  R+ P   +RP +    ES+++ K + EFY+++++P+V+ FLD+E+ PWK
Sbjct: 421 VLILRGNSFEVRRLP---ERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEKSPWK 480

Query: 481 EWVEKLDEYTGIPNEQLDEVRNAWKAW 499
           EWV+ LD   G+ +E+L++VR  W+ W
Sbjct: 481 EWVDHLDRNDGLIDEELEDVRKGWEEW 480

BLAST of Lsi02G012250 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 124.0 bits (310), Expect = 3.4e-28
Identity = 113/397 (28.46%), Postives = 182/397 (45.84%), Query Frame = 0

Query: 58  RKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLN 117
           R+   +R++KV +   + G  Y+G+Q   +     TIE ELE A++ AG + +++     
Sbjct: 50  RRAPKYRRRKVAIVFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPDADRNKPR 109

Query: 118 KISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVP 177
              WARS+RTDKGV ++   ++ +  +       DP G      +N  LPD I+VFG   
Sbjct: 110 NYEWARSARTDKGVSAVGQVVSGRFYV-------DPPG--FVERLNSKLPDQIRVFGYKR 169

Query: 178 SQRSFDPRKECDVRKYSYLLPA----KLIGIESKLKTTE--------VDCHISDFKDILN 237
              SF  +K CD R+Y YL+P       +  E+++  T+        V+C    +K I  
Sbjct: 170 VAPSFSSKKFCDRRRYVYLIPVFALDPCVHGEAEMVRTDLGYEYVKCVECSEKGYK-IPL 229

Query: 238 SFQGEHPFHNYTIRSKYRTKVRAK--QSPGYDHKSGNEVS--PSDVSNSEFEESDGEDDT 297
              G+      T   + ++ + +    + G D K     S  PS  +N   E  DG D +
Sbjct: 230 GVMGKDTDCCDTKSLEIQSDISSSTCDAIGADVKCETLSSSVPSAENNLNSEVLDGADVS 289

Query: 298 FVEGVKEPDQRSPSLSVCYENPTEVCSKLGDGSELPKGVRARWLHEPDEKDR-------- 357
            +    E  + S +L+       +V    G+G ++ K   +++ +   EK+R        
Sbjct: 290 ALSSKAEGMEESNTLA-------KVEMNNGEGEDMTKS-ESKFCYGEKEKERFNRILSYY 349

Query: 358 IGASHF---------------RKILCCSCGKLETSFGYDFIEISIWGESFMLHQIRKMIG 416
           +G+ +F               R IL  +   +    G DF++  + G+SFMLHQIRKMIG
Sbjct: 350 VGSHNFHNFTTRTKAADPAANRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQIRKMIG 409

BLAST of Lsi02G012250 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-24
Identity = 104/383 (27.15%), Postives = 169/383 (44.13%), Query Frame = 0

Query: 66  KKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSS 125
           +KV +   + G  Y+G+Q   +     TIE ELE A++ AG + ES  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 126 RTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDPR 185
           RTDKGV ++        ++    ++ DP G    N +N  LP+ I++FG      SF  +
Sbjct: 82  RTDKGVSAVG-------QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 186 KECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSKYRTK 245
           K CD R+Y YLLP   +   S      V   +   ++ +  F+          R +    
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSE-------RGRKIPP 201

Query: 246 VRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYENPTE 305
               +  G +  + +    SD+S++    S    D  +E +        S+ V      E
Sbjct: 202 GLVGKWKGTNFGTKSLDFQSDISSN--NSSALRSDIKIEALSSNLAGLCSVDVEVGRIQE 261

Query: 306 VCSKLGDGSELPKGVRARWLHEPDEKDR--------IGASHF---------------RKI 365
              KL   S   K V++++ +   EK+R        +G+ +F               R+I
Sbjct: 262 DSCKLNTNSSETK-VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQI 321

Query: 366 LCCSCGKLETSFGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTKFSR 425
           +  +   +    G DFI+  + G+SFMLHQIRKM+G AVA+ R      +I  + +K   
Sbjct: 322 ISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFSKDVN 381

BLAST of Lsi02G012250 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 104.0 bits (258), Expect = 3.6e-22
Identity = 96/357 (26.89%), Postives = 157/357 (43.98%), Query Frame = 0

Query: 66  KKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARSS 125
           +KV +   + G  Y+G+Q   +     TIE ELE A++ AG + ES  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 126 RTDKGVHSLATTITLKMEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQRSFDPR 185
           RTDKGV ++        ++    ++ DP G    N +N  LP+ I++FG      SF  +
Sbjct: 82  RTDKGVSAVG-------QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 186 KECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPFHNYTIRSKYRTK 245
           K CD R+Y YLLP   +   S      V   +   ++ +  F+          R +    
Sbjct: 142 KFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSE-------RGRKIPP 201

Query: 246 VRAKQSPGYDHKSGNEVSPSDVSNSEFEESDGEDDTFVEGVKEPDQRSPSLSVCYENPTE 305
               +  G +  + +    SD+S++    S    D  +E +        S+ V      E
Sbjct: 202 GLVGKWKGTNFGTKSLDFQSDISSN--NSSALRSDIKIEALSSNLAGLCSVDVEVGRIQE 261

Query: 306 VCSKLGDGSELPKGVRARWLHEPDEKDR--------IGASHF---------------RKI 365
              KL   S   K V++++ +   EK+R        +G+ +F               R+I
Sbjct: 262 DSCKLNTNSSETK-VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQI 321

Query: 366 LCCSCGKLETSFGYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDIIALSLTK 400
           +  +   +    G DFI+  + G+SFMLHQIRKM+G AVA+ R      +I  + +K
Sbjct: 322 ISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFSK 356

BLAST of Lsi02G012250 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 56.6 bits (135), Expect = 6.6e-08
Identity = 50/183 (27.32%), Postives = 74/183 (40.44%), Query Frame = 0

Query: 65  KKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYGNLNKISWARS 124
           K+ V L+I Y G  + G     E Q  P+IE E    ++KA        G+     ++R 
Sbjct: 89  KRYVALKIMYFGKRFYG--FSAEAQMEPSIESE----VFKALERTRLLVGDKKDSCYSRC 148

Query: 125 SRTDKGVHSLATTITL----KMEIPE-------SAWMGDPCGISLANYINFYLPDNIKVF 184
            RTDKGV S    I L    +++ P        +   G+         +N  LPD+I+V 
Sbjct: 149 GRTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVI 208

Query: 185 GIVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEVDCHISDFKDILNSFQGEHPF 237
           G  P+   F  R  C  R+Y Y    + + + +            DF      F GEH F
Sbjct: 209 GWSPAPIDFHARFSCYAREYKYFFWRQNLNLSA-----------MDFAG--KKFIGEHDF 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O229284.4e-14252.27Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032... [more]
Q59S631.9e-2327.23tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876... [more]
Q6CC393.2e-2328.12tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O... [more]
Q755C84.6e-2230.62tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51... [more]
Q6FV055.1e-2127.23tRNA pseudouridine synthase 1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / ... [more]
Match NameE-valueIdentityDescription
A0A1S3C5W17.1e-26088.21putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=... [more]
A0A6J1I1567.6e-25486.14putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569... [more]
A0A6J1HM833.5e-25185.32putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114648... [more]
A0A6J1DMV69.0e-24784.13putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DQ079.0e-24784.13putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_038894774.14.6e-26990.57putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida][more]
XP_008457308.11.5e-25988.21PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo][more]
XP_022970646.11.6e-25386.14putative tRNA pseudouridine synthase [Cucurbita maxima][more]
XP_023520301.16.6e-25285.91putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo][more]
XP_022964935.17.3e-25185.32putative tRNA pseudouridine synthase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G30320.13.2e-14352.27Pseudouridine synthase family protein [more]
AT1G20370.13.4e-2828.46Pseudouridine synthase family protein [more]
AT1G76120.11.0e-2427.15Pseudouridine synthase family protein [more]
AT1G76120.23.6e-2226.89Pseudouridine synthase family protein [more]
AT1G34150.16.6e-0827.32Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 300..415
e-value: 2.1E-6
score: 28.2
IPR020094Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminalGENE3D3.30.70.580coord: 57..188
e-value: 1.3E-31
score: 111.2
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3D3.30.70.660coord: 189..486
e-value: 2.9E-34
score: 120.3
NoneNo IPR availablePIRSRPIRSR001430-2PIRSR001430-2coord: 118..247
e-value: 1.6E-15
score: 55.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 249..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..270
NoneNo IPR availablePANTHERPTHR11142:SF21TRNA PSEUDOURIDINE SYNTHASEcoord: 40..500
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 40..500
IPR041708Pseudouridine synthase PUS1/ PUS2-likeCDDcd02568PseudoU_synth_PUS1_PUS2coord: 70..420
e-value: 2.60458E-79
score: 245.986
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 336..422
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 65..240

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G012250.1Lsi02G012250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031119 tRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding