Lsi02G012050 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G012050
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein NETWORKED 4A
Locationchr02: 15475141 .. 15480599 (+)
RNA-Seq ExpressionLsi02G012050
SyntenyLsi02G012050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGGAAGCATGGTTGGGAAAATGGGGGAAAAGAAGTGCAGCAGTGTCTGTGTGTTGATTGTTGTCAATCAGAGCTCCACCTCTTCCACTCAGTTTTGTTCCTCTTCGATCTCTCCAATAACAGAATAAGAATATGCGATACAGCCATTGAATTACAGTAAGCTTTGTTCACTCCTTATCTCTCCTTTCTTATTTGTATTGCCATTTCACTTCACTCCTCTCCTTTTTATTTCCACCCACAAAGCTTTGCCGATCAGATTCCCACCCTCTTCTACTTCTACTTTCCTAGATCTTTCCCCAATTTTCCTGTTATGTCAGTGCACTACCACATTTTATAATATCTTCTTATGGATGGCTATATATATCGCGATCTTTTTTCTTTTTTTTTACAGTTTGTTGTATTTTTTACCGTAAATGTTGTGTAATCATCGTCATCACGTTAGAGTTACGATAGACATGACTATCCTATATGTTATAAAATACGTACACAACGTTATAAAATACGATAGTATACTTTGACGTAAGTAATAGCTTAGATTTCATACCTCTCTCATTTTCCTTGCATTTAGAATTACTAGTGCTTGAGTTCTTTGGCATCGGGGTAAATGGAAATGACCACATTAGGTTAATTTTTGGGAAGTAACTTAATTTGTGTTTGGGAATGGGATGAGATTAAATTAGGATGTAAAGGAGTGTATATATACATGTTTGAACGGCGGGTAAACTATTAGCGTGTGATGGTGTAAATGTATTGTATATTTGTATTAAAGAGGGAGGTGAGGTGGGAGTGTATTAAATTAAAAGCGAACGCGTGGACTACATAGTCTGAACTGGAGGTGTGTTGCGGACAGTTGTAATAATAGTCAAATCCTCCGCCTGCATATAGTTCTGCTAAATTTGCTAATGAAATTAATTCATTTTAAACCTAAACTCTATTCTATTCCTTCACCTTCACTCACACAAGAAACCATATTTACTCTTTAATGGGCCTTAAAAAGCCCACTTCCCATTTCTATTGTATTCATCCTTCTGCTTCTACTTCTACCTACCTACCCACCTCCAGTTCAGCCATAGCTGATTTTATTTGAATCATTTCATTTGAATTTGTTCAGAAAGTAAAGTGGGTTTCTGTTTTCTTTCTCTTTGGATCGTATGTTTTCACTTTGCTGAAGCTGCCGAAGGGATTACGGACGACGGAGGAAGGCTTCGTTGGCTTCCCCTCTCTAGCCTGAGTCAACCTCCAACTTCAGGCTTTCTTCGGCTCAATGGCCAACTCATTGGTATGCTTTATACTTTATAATCATCACTTCAAAATCCATTCTCTTCTTTTTTTTCGNTTTCTTGATCTCAGGCTCGGAGCGATAGGAAATTCAAGAGGTTGGAATCTCGGAAGTCTCATTCTTGGTGGTGGGACAGTCACGTCAGCCCCAAAAACTCTAGATGGCTCATGGAGAATCTCGAGGGTTAGTTACTGCTTTTCTTCTATTACTTAATCCTTTTGGGTTTGTGATCTTAGGAGTTGAGTTATTGCTGGGGACATTTATATTTGTTTTTCCATGTATTATCTTAGTAATATTGTTTAGTTTGTTTAAATAAATCTGTGAATTGTATTTTTATAGGATAAAACATTAGAGAACTAGATTTTTAAATAACTTTTTAGTTTTGTGATTGAAGAACTTATTTAGAGAATATGTAAATTCATAGTCGATAAACAAGTGCTTCTGCATTTCAACCAATACTGAACACTTTTGAACTCATTTCAGCTATAACTTTTATTGTAAATTTGCAGTCATTTCTATTATAGCTTTTAGTTTACTTTAACTATGCATTATTATTTTAATGAAATTAGACTGTTTTTGGTTTTTGGTTTTCAGCTTTTAAGAAATGATCAAAACAATACGTATTTTTTTTATTTTTATTTTAGTACAACGGCAGGGATGAGTGGGGTATCAAACCTTCGGCTTCTAGGGAATAAGATCATGCAGATTACTATGGAGATAAGCTCACTCTGGCAAAGAGGGCTTGTTTTAATAAACTAATTAACAAGTTTTTTATTCATTCTAGTAAAGTAATAAAGAATAATAATAAGGAATGAAGCCTTGATTATGTATATTTTTTTTCTTTATCATAGGAACCAAAATAATAAATCTTCTTGTGATTCTTGTTCTTTCGATTTTTTTTTTTTTTGTTTAAAAAATATTTTGTGTTTTCAAATAAAATAAATAATTTTATCAATGATTCATTGGAACAAAAAAAAAAAAAAGCTAACGACCAGACCTTGGAACTAAAATAAAAGGACTTTTCGAATGAAATAAAATAAGATAGATATTATTTTGTTTTATTTATCAATATTTTTAGTAATATTTAATACATTACTAAAAATTATGGAAAAAAAAAAAACCTTTGAGCAACATATAACTTCTCTTACATGATCATCATGATAGATGCTAGATAATAGCTTCGAGTTCCCAAATTGCTTTGGGCTGGTATTGCACAGATAGCAACTCCCTCTTTGTTTTAGGATATCACTTTCCTCCTTTGTGTTCGTTTTCGCTGGGAGTGCTAAAAAAATCAAGGTATTATTAGGTATATTGGGAGTGCTGCAATATATAGAGAATCTTGTTATGGCTTTCCTTTCTTTTGCTATATTTGTCTTTATGCTGTTTCTTTGTAACCTTTTCCCACTCAAATATCCCGGCCGCGTCTTGCCAAATAACATCTAAGGAAATTGTTCTCCTCTAATTCTCTGTTGACATGACATCTGTTTGCATTATGCTAGAAAATTTCCTTTTATTTAACAGTTCTCCCCATTTTTCAATTCATATTCTAAAAGGGGTGGGGTTAAATTTGGGAATGTGGGTAGCAGTTGCAAAATTTTAGCTTCTTCCTCCTTTCTGCATGAATCTTATGGTGCATGTGTTTCAACTACCCAAACATTGGCATGGAATTAGTTTTGTAAGCTCCATGTTTTATTTCTGCAATTCAACAATAGGTGAAAGTCAATGTTAATTCTTCATCAAAATGCTAAACAAAAAATTCACAAAACGATCCTTGATGTCAGAGATGGACCGCAGCATCAAGCGTATGTTGAAGCTAATAGAAGAGGATGCAGATTCATTTGCTAAGAAGGCTGAAATGTATTATCAGAAGAGACCAGTGTTGATCTCTCATGTTGAGGAATTCTACCGCATGTATCGTTCCCTTGCTGAGCGTTATGATCATGTGACAGGAGAGCTTAGGAAAAATATTCCTTCAGATCTACAATCTCAGGGCTCTGGTATTTCTGACCTGGGCTCTGAGCCGTCCACTACATGGCCCTCTCCTGATCAAAGGCTTGGACGTCGGAAATCTGGACCTCGAGCTGCTGGCTTTGAATTCTTCCTTGGATCTGGTGGAAGCAACTCTGATACTTGCCAAAAGGAAGGAGATGAGTCATCTTCCTTGACAGAATCTGAACCTGAATCTGACGATTCCTCGGTAAACAATTATTCAGGTGGTGATCAAGGTCTGAACAGGAAGATGATTGAATTGGAGATTGAGCTCCGTGAGGCTAAAGAAAAGCTCCGCATGAAAGAGGATAATGCAGAAGGTTCATTTCCATTCAAGGGGCCAACAGATGAAAATTCTGATTATGCTTTTGCTAGAATTGCTGGGTACGAGGAAGAATTAAGGAATGCAAATGAGAAGCTGCGAATTTCAGATGCACAGATCACGAGGTTGAAAAGTGAGCTCCAAAAGTACCGGCAATCAGAATTGACCAAAGGTTTGCAGGCTGAGTCTGAGTCTGTGTCTGCTACAATGGAAGATAACCAGAGGCATGAGGATGGGGTTCCTTTGGTCATTAACCAGGAATCAGAGGTTGACGAACATCATAGAGGTTCGGGAGGTGACCAAGTCATTACCGTCGAGGGATTGGTGGAAGAGCAAAAAATTACAAAGGAAAGACTCGAGAACTCGCAGAAAGAAGTTTCTAAATTAAAGCTTGAACTTGAGAACAATAGATCACCTGAGAAAATCACCCATTTGCAGAATGAGCTTGAAGCTGCCCTAAAAGATACCACTACATGGAAGGCCAAGCTGAGTGCAGAGAGAAGAGAGGTGTCCAAGCTCCAAGAAAGAATTTCAAGGTTGAAAGGTAGTTTATCGGATCGAGATCATGAGATCAGGGATTTAAAACTATCAGTATCTGATGCCGAGCAGAAAATCTTTCCCGAGAAAGCACAGGTTAAGGCTGAAATGTCTAAGCTACTTGAGGAACGAGCTCTGTTGATGGAACAAGTCAGAGAATGGGAACACGGAGCTCGTCTTTTGGAGGACGAAATCAGAAAAGTCAAGGGGGAAAAGGTGGTTTTGGAGGAGAGACTTAACGGTGAGATCGAGCGGTTGGAAACGACAATTGTTGAGAAAGTAGAATACATTGAAAATCTCAAAAATGGGCTTAATGATTTGCAATCAGAGAGAGATGAGCTCCATTCAGAAGTTGTTACTTTAAAAGAGAATTTGAGCTCTAAAGAGAAGCAGGTAGATGAAATAAGGAAGCATGTGCAAAAATTGGAAAGGGAACGTGTGGAGCTAGTATCAAATATTGACAAAGCAGACAAGATGGCAGAGGAATTAAGATTAAGAGAGAAGGAGCTAGAAGGGGAGGTGGAAAAGCAAAGAATACTGATAATGGAAGGAGCAGAGGAAAAGAGAGAGGCTATAAGGCAGCTATGTTTATCGATTGATCACTACAGGAGTGGATATCATATGCTTAGAGAAGTTTTTATTGGTCAAAAGCGGGTTCCAGTGTTGGCTTCTTAACGTAAGTACATGTTTAGAATCTCAAGGAGTTGTTGCCTTTGTTTCTGGGATGAAGAAACAAACAGTTAAAATCCAGAGTTTGCTACAAATTGGATCATCATACGGTTTTTCTGTTTGTTAAATTAAATGATGTAAATGTAGTTGTTTAGAGTAACAATGAGCGGGGAGCTGCCGATGGTTACGCCCCCCTGGGTACTCTGCCGTGATTCGTGAATGGTCCCGGAGGGTTGAGAGACGGAACTCTCTACTTTGGCGGCCTCTCGCCGAACTTTGCTCTTGTAATCGTCCATCGAACTATCAACACTTTTAGAAGACGACTGTGAACCAAGCAAATGAGCCATATTCTACGCCCTTGTATTTTGTTGTGTATGAGACGCCAGGATTGAATCATTTTAACAAACATCGGCTCGTTGGTTATTAACCTTCCTTCAATGTCTACCTGTTGCTCTTTAATGTTTATTTAAGAAGAGAAACTGTATATGAGGAACTATTTAGGACAAGCATGGCTCTTTAATTAGGACAATATTACAGTTTTTAATTAGGAAAAGGATGGCTTTTCTTGTTTTTTTTGGAAAAAATTTGATGGAATAGGGCCACGTTTACTTAAAATTGGTGTAATCGTTTAGGACAAGCACGAACCAAAACTATGGAGTAAG

mRNA sequence

ATTGGAAGCATGGTTGGGAAAATGGGGGAAAAGAAGTGCAGCAGTGTCTGTGTGTTGATTGTTGTCAATCAGAGCTCCACCTCTTCCACTCAGTTTTGTTCCTCTTCGATCTCTCCAATAACAGAATAAGAATATGCGATACAGCCATTGAATTACACTGCCGAAGGGATTACGGACGACGGAGGAAGGCTTCGTTGGCTTCCCCTCTCTAGCCTGAGTCAACCTCCAACTTCAGGCTTTCTTCGGCTCAATGGCCAACTCATTGGCTCGGAGCGATAGGAAATTCAAGAGGTTGGAATCTCGGAAGTCTCATTCTTGGTGGTGGGACAGTCACGTCAGCCCCAAAAACTCTAGATGGCTCATGGAGAATCTCGAGGATGGACCGCAGCATCAAGCGTATGTTGAAGCTAATAGAAGAGGATGCAGATTCATTTGCTAAGAAGGCTGAAATGTATTATCAGAAGAGACCAGTGTTGATCTCTCATGTTGAGGAATTCTACCGCATGTATCGTTCCCTTGCTGAGCGTTATGATCATGTGACAGGAGAGCTTAGGAAAAATATTCCTTCAGATCTACAATCTCAGGGCTCTGGTATTTCTGACCTGGGCTCTGAGCCGTCCACTACATGGCCCTCTCCTGATCAAAGGCTTGGACGTCGGAAATCTGGACCTCGAGCTGCTGGCTTTGAATTCTTCCTTGGATCTGGTGGAAGCAACTCTGATACTTGCCAAAAGGAAGGAGATGAGTCATCTTCCTTGACAGAATCTGAACCTGAATCTGACGATTCCTCGGTAAACAATTATTCAGGTGGTGATCAAGGTCTGAACAGGAAGATGATTGAATTGGAGATTGAGCTCCGTGAGGCTAAAGAAAAGCTCCGCATGAAAGAGGATAATGCAGAAGGTTCATTTCCATTCAAGGGGCCAACAGATGAAAATTCTGATTATGCTTTTGCTAGAATTGCTGGGTACGAGGAAGAATTAAGGAATGCAAATGAGAAGCTGCGAATTTCAGATGCACAGATCACGAGGTTGAAAAGTGAGCTCCAAAAGTACCGGCAATCAGAATTGACCAAAGGTTTGCAGGCTGAGTCTGAGTCTGTGTCTGCTACAATGGAAGATAACCAGAGGCATGAGGATGGGGTTCCTTTGGTCATTAACCAGGAATCAGAGGTTGACGAACATCATAGAGGTTCGGGAGGTGACCAAGTCATTACCGTCGAGGGATTGGTGGAAGAGCAAAAAATTACAAAGGAAAGACTCGAGAACTCGCAGAAAGAAGTTTCTAAATTAAAGCTTGAACTTGAGAACAATAGATCACCTGAGAAAATCACCCATTTGCAGAATGAGCTTGAAGCTGCCCTAAAAGATACCACTACATGGAAGGCCAAGCTGAGTGCAGAGAGAAGAGAGGTGTCCAAGCTCCAAGAAAGAATTTCAAGGTTGAAAGGTAGTTTATCGGATCGAGATCATGAGATCAGGGATTTAAAACTATCAGTATCTGATGCCGAGCAGAAAATCTTTCCCGAGAAAGCACAGGTTAAGGCTGAAATGTCTAAGCTACTTGAGGAACGAGCTCTGTTGATGGAACAAGTCAGAGAATGGGAACACGGAGCTCGTCTTTTGGAGGACGAAATCAGAAAAGTCAAGGGGGAAAAGGTGGTTTTGGAGGAGAGACTTAACGGTGAGATCGAGCGGTTGGAAACGACAATTGTTGAGAAAGTAGAATACATTGAAAATCTCAAAAATGGGCTTAATGATTTGCAATCAGAGAGAGATGAGCTCCATTCAGAAGTTGTTACTTTAAAAGAGAATTTGAGCTCTAAAGAGAAGCAGGTAGATGAAATAAGGAAGCATGTGCAAAAATTGGAAAGGGAACGTGTGGAGCTAGTATCAAATATTGACAAAGCAGACAAGATGGCAGAGGAATTAAGATTAAGAGAGAAGGAGCTAGAAGGGGAGGTGGAAAAGCAAAGAATACTGATAATGGAAGGAGCAGAGGAAAAGAGAGAGGCTATAAGGCAGCTATGTTTATCGATTGATCACTACAGGAGTGGATATCATATGCTTAGAGAAGTTTTTATTGGTCAAAAGCGGGTTCCAGTGTTGGCTTCTTAACGTAAGTACATGTTTAGAATCTCAAGGAGTTGTTGCCTTTGTTTCTGGGATGAAGAAACAAACAGTTAAAATCCAGAGTTTGCTACAAATTGGATCATCATACGGTTTTTCTGTTTGTTAAATTAAATGATGTAAATGTAGTTGTTTAGAGTAACAATGAGCGGGGAGCTGCCGATGGTTACGCCCCCCTGGGTACTCTGCCGTGATTCGTGAATGGTCCCGGAGGGTTGAGAGACGGAACTCTCTACTTTGGCGGCCTCTCGCCGAACTTTGCTCTTGTAATCGTCCATCGAACTATCAACACTTTTAGAAGACGACTGTGAACCAAGCAAATGAGCCATATTCTACGCCCTTGTATTTTGTTGTGTATGAGACGCCAGGATTGAATCATTTTAACAAACATCGGCTCGTTGGTTATTAACCTTCCTTCAATGTCTACCTGTTGCTCTTTAATGTTTATTTAAGAAGAGAAACTGTATATGAGGAACTATTTAGGACAAGCATGGCTCTTTAATTAGGACAATATTACAGTTTTTAATTAGGAAAAGGATGGCTTTTCTTGTTTTTTTTGGAAAAAATTTGATGGAATAGGGCCACGTTTACTTAAAATTGGTGTAATCGTTTAGGACAAGCACGAACCAAAACTATGGAGTAAG

Coding sequence (CDS)

ATGGACCGCAGCATCAAGCGTATGTTGAAGCTAATAGAAGAGGATGCAGATTCATTTGCTAAGAAGGCTGAAATGTATTATCAGAAGAGACCAGTGTTGATCTCTCATGTTGAGGAATTCTACCGCATGTATCGTTCCCTTGCTGAGCGTTATGATCATGTGACAGGAGAGCTTAGGAAAAATATTCCTTCAGATCTACAATCTCAGGGCTCTGGTATTTCTGACCTGGGCTCTGAGCCGTCCACTACATGGCCCTCTCCTGATCAAAGGCTTGGACGTCGGAAATCTGGACCTCGAGCTGCTGGCTTTGAATTCTTCCTTGGATCTGGTGGAAGCAACTCTGATACTTGCCAAAAGGAAGGAGATGAGTCATCTTCCTTGACAGAATCTGAACCTGAATCTGACGATTCCTCGGTAAACAATTATTCAGGTGGTGATCAAGGTCTGAACAGGAAGATGATTGAATTGGAGATTGAGCTCCGTGAGGCTAAAGAAAAGCTCCGCATGAAAGAGGATAATGCAGAAGGTTCATTTCCATTCAAGGGGCCAACAGATGAAAATTCTGATTATGCTTTTGCTAGAATTGCTGGGTACGAGGAAGAATTAAGGAATGCAAATGAGAAGCTGCGAATTTCAGATGCACAGATCACGAGGTTGAAAAGTGAGCTCCAAAAGTACCGGCAATCAGAATTGACCAAAGGTTTGCAGGCTGAGTCTGAGTCTGTGTCTGCTACAATGGAAGATAACCAGAGGCATGAGGATGGGGTTCCTTTGGTCATTAACCAGGAATCAGAGGTTGACGAACATCATAGAGGTTCGGGAGGTGACCAAGTCATTACCGTCGAGGGATTGGTGGAAGAGCAAAAAATTACAAAGGAAAGACTCGAGAACTCGCAGAAAGAAGTTTCTAAATTAAAGCTTGAACTTGAGAACAATAGATCACCTGAGAAAATCACCCATTTGCAGAATGAGCTTGAAGCTGCCCTAAAAGATACCACTACATGGAAGGCCAAGCTGAGTGCAGAGAGAAGAGAGGTGTCCAAGCTCCAAGAAAGAATTTCAAGGTTGAAAGGTAGTTTATCGGATCGAGATCATGAGATCAGGGATTTAAAACTATCAGTATCTGATGCCGAGCAGAAAATCTTTCCCGAGAAAGCACAGGTTAAGGCTGAAATGTCTAAGCTACTTGAGGAACGAGCTCTGTTGATGGAACAAGTCAGAGAATGGGAACACGGAGCTCGTCTTTTGGAGGACGAAATCAGAAAAGTCAAGGGGGAAAAGGTGGTTTTGGAGGAGAGACTTAACGGTGAGATCGAGCGGTTGGAAACGACAATTGTTGAGAAAGTAGAATACATTGAAAATCTCAAAAATGGGCTTAATGATTTGCAATCAGAGAGAGATGAGCTCCATTCAGAAGTTGTTACTTTAAAAGAGAATTTGAGCTCTAAAGAGAAGCAGGTAGATGAAATAAGGAAGCATGTGCAAAAATTGGAAAGGGAACGTGTGGAGCTAGTATCAAATATTGACAAAGCAGACAAGATGGCAGAGGAATTAAGATTAAGAGAGAAGGAGCTAGAAGGGGAGGTGGAAAAGCAAAGAATACTGATAATGGAAGGAGCAGAGGAAAAGAGAGAGGCTATAAGGCAGCTATGTTTATCGATTGATCACTACAGGAGTGGATATCATATGCTTAGAGAAGTTTTTATTGGTCAAAAGCGGGTTCCAGTGTTGGCTTCTTAA

Protein sequence

MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRKNIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKEGDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPFKGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESESVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQKEVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSLSDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEIRKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENLSSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGAEEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS
Homology
BLAST of Lsi02G012050 vs. ExPASy Swiss-Prot
Match: F4KEW8 (Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 7.0e-107
Identity = 270/580 (46.55%), Postives = 353/580 (60.86%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRS+KRM+KLIEEDADSFAKKAEMYYQ RP LI+ V+EF+RMYR+LAERY+++TGELRK
Sbjct: 44  MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 103

Query: 61  NIPSDLQSQGSGISDL-GSEPSTTWPSPD-QRLGRRKSGPRAAGFEFFLGSGGSNSDTCQ 120
             P +LQSQGSG+SD+  S+ S  W S +  RLGR  SG RA GFE+FLG+GG  SD   
Sbjct: 104 GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 163

Query: 121 KEGDESSSLTESEPESDDSSVNNYSG------GDQGLNRKMIELEIELREAKEKLRMK-E 180
           K+GD+S+S+T+SE ESDDSSV NY G        Q L++++++LEIELREAKE+LRM+ E
Sbjct: 164 KDGDDSASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRMQLE 223

Query: 181 DNAEGSFPFKGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSEL 240
            N E   P      +  D+  A++A  E+EL++ NEKL+ S+ QI  LKS+L +Y    L
Sbjct: 224 GNTESLLPRVKSETKFVDFP-AKLAACEQELKDVNEKLQNSEDQIYILKSQLARY----L 283

Query: 241 TKGLQAESESVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKIT 300
             GL                               DE   G+   Q + +E L EE +IT
Sbjct: 284 PSGLD------------------------------DEQSEGAASTQELDIETLSEELRIT 343

Query: 301 KERLENSQKEVSKLKLELENNRSPE-KITHLQNELEAALKDTTTWKAKLSAERREVSKLQ 360
             RL  ++K+   ++ E+E ++S + K+  LQ+ LE+A K+   WK+K SA++REV KL 
Sbjct: 344 SLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQDMLESAQKEAAAWKSKASADKREVVKLL 403

Query: 361 ERISRLKGSLSDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWE 420
           +RIS LK SL+ RDHEIRDLK ++SDAE+KIFPEKAQVKA+++KLLEE+    +Q +E E
Sbjct: 404 DRISMLKSSLAGRDHEIRDLKTALSDAEEKIFPEKAQVKADIAKLLEEKIHRDDQFKELE 463

Query: 421 HGARLLEDEIRKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELH 480
              R LEDE RKV  EK+  EE+L  EIE L    VEK   IE L   +++L+SE   L 
Sbjct: 464 ANVRYLEDERRKVNNEKIEEEEKLKSEIEVLTLEKVEKGRCIETLSRKVSELESEISRLG 523

Query: 481 SEVVTLKENLSSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVE 540
           SE+           K  D+                               R  E+E EVE
Sbjct: 524 SEI-----------KARDD-------------------------------RTMEMEKEVE 546

Query: 541 KQRILIMEGAEEKREAIRQLCLSIDHYRSGYHMLREVFIG 571
           KQR  + E AEEKRE IRQLC S+D+ R  Y  LR  F G
Sbjct: 584 KQRRELEEVAEEKREVIRQLCFSLDYSRDEYKRLRIAFSG 546

BLAST of Lsi02G012050 vs. ExPASy Swiss-Prot
Match: Q84VY2 (Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.1e-88
Identity = 247/582 (42.44%), Postives = 332/582 (57.04%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MD  +  MLKLIEEDADSFAKKA+MY+QKRP LI  VEEFYRMYR+LAERYD  +GEL+K
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           N  S++QSQ S   ++ S          ++L RR+S                      KE
Sbjct: 104 NHTSEIQSQSS--LEISSPTK-------EKLSRRQSS--------------------HKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
            ++SSSLT+S  +SD SS N+   GD+ L R+M ELE+EL+E K+KL +++++ +G    
Sbjct: 164 EEDSSSLTDSGSDSDHSSAND-EDGDEALIRRMAELELELQETKQKLLLQQESVDG---- 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
               D N D    +I  YE EL+ ANEK+R+ + +I  LK++LQ +   +    L AE +
Sbjct: 224 ----DNNVD-LLHKITTYEGELKEANEKMRMHEDEIANLKNQLQSFMSFDTEDHLGAEQK 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           SV    ED +  ED V                        V  L EE  I KE+L++ +K
Sbjct: 284 SVDLDKEDTK--EDAV---------------------ATKVLALEEELSIAKEKLQHFEK 343

Query: 301 EVSKLKLELE-NNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGS 360
           E   LK ELE    + EK+  LQ+ELE A +D  T+  KL+AE++EV KLQER++ +K S
Sbjct: 344 ETYSLKNELEIGKAAEEKLKSLQHELELAQRDADTYINKLNAEKKEVLKLQERLAMVKTS 403

Query: 361 LSDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDE 420
           L DRD+EIR LK +VSDAEQKIFPEKAQ+K EMSK+LEER+ L EQ+RE       LE  
Sbjct: 404 LQDRDNEIRALKTAVSDAEQKIFPEKAQIKGEMSKMLEERSQLGEQLRE-------LESH 463

Query: 421 IRKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKEN 480
           IR +K EK   EE+L G  E++               +G+ D          E   L+E 
Sbjct: 464 IRLIKEEKAETEEKLRGGTEKI---------------SGMRD----------ESNVLREE 517

Query: 481 LSSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEG 540
           +  +E+++ E  KH+++L  E+V               LR R  EL  EVE+ R+   E 
Sbjct: 524 IGKREEKIKETEKHMEELHMEQV--------------RLRRRSSELTEEVERTRVSASEM 517

Query: 541 AEEKREAIRQLCLSIDHYRSGYHMLREVFIG--QKRVPVLAS 580
           AE+KREAIRQLC+S+DHYR GY  L  V  G   KRV VL++
Sbjct: 584 AEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVVVLST 517

BLAST of Lsi02G012050 vs. ExPASy Swiss-Prot
Match: F4HZB5 (Protein NETWORKED 1D OS=Arabidopsis thaliana OX=3702 GN=NET1D PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.8e-15
Identity = 138/569 (24.25%), Postives = 247/569 (43.41%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELR- 60
           MD  +K+M+K+IEEDADSFA++AEMYY+KRP L+  VEEFYR YR+LAERYDH TG +R 
Sbjct: 35  MDSKVKQMIKVIEEDADSFARRAEMYYKKRPELMKLVEEFYRAYRALAERYDHATGVIRH 94

Query: 61  ------KNIPSDLQSQGSGISDLGS-----EPSTTWPSPDQRLGRRKSGPRAAGFEFFLG 120
                 +  P+         S LGS     +P T    P  R        R   F    G
Sbjct: 95  AQQTMAEAFPNQDPMMFGEESPLGSSTDGFDPQTPDSYPPIRAPVYPDDLRKGAF----G 154

Query: 121 SGGSNSDTCQK-----EGDESSSLTESEPESDDSSVNNYSGGD-QGLNRKMIELEIELRE 180
              S+  T ++     E  +S S  +    +      N++  D + +N K++       +
Sbjct: 155 ISSSHLSTVKRNIAFMEDPQSVSSGKGFKTAKARKGLNFNNVDGKEINAKVLSESERASK 214

Query: 181 AKEKLRMKEDNAEGSFPFKGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSE 240
           A+ ++   +D        K  +    D    +++  E E+  A E  R+   + TR ++E
Sbjct: 215 AEAEIVALKDALSKVQAEKEASLAQFDQNLEKLSNLESEVSRAQEDSRVLIERATRAEAE 274

Query: 241 LQKYRQSELTKGLQAESE--SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVIT 300
           ++  R+S     ++ ES        +++    ED + L   +  EVDE    +  + +  
Sbjct: 275 VETLRESLSKVEVEKESSLLQYQQCLQNIADLEDRISLAQKEAGEVDERANRAEAETLAL 334

Query: 301 VEGLVEEQKITKERLENSQ---KEVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKA 360
            + LV  +   +  L   Q   K +S L+  L       ++T+ + E           + 
Sbjct: 335 KQSLVSSETDKEAALVQYQQCLKTISNLEERLHKAEEDSRLTNQRAE---------NAEG 394

Query: 361 KLSAERREVSKLQERISRLKGSLSDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKL-- 420
           ++ + +++VSKL E     +         I DLKL +  A+++      +++  ++KL  
Sbjct: 395 EVESLKQKVSKLIEENEAYELQYQQCLDTIADLKLKLFHAQEETQRLSREIEDGVAKLKF 454

Query: 421 LEERALLMEQVREWEHGARLLEDEIRKVKGEKVVLEERLNGEIERLETTIVEKVEYIENL 480
            EE+ +++E+  +  H    L+  + K+  +   L E+   E+ RL T + E+       
Sbjct: 455 AEEKCVVLERSNQNLHSE--LDGLLEKLGNQSHELTEK-QKELGRLWTCVQEENLRFMEA 514

Query: 481 KNGLNDLQSERDELHSEVVTLKENLSSK--------------EKQVDEIRKHVQKLERER 531
           +     LQ    +   E+ TL   L ++              +++V E +   + L    
Sbjct: 515 ETAFQTLQQLHSQSQEELSTLALELQNRSQILKDMEARNNGLQEEVQEAKDQSKSLNELN 574

BLAST of Lsi02G012050 vs. ExPASy Swiss-Prot
Match: Q9LUI2 (Protein NETWORKED 1A OS=Arabidopsis thaliana OX=3702 GN=NET1A PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.0e-13
Identity = 148/621 (23.83%), Postives = 263/621 (42.35%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGEL-- 60
           MD  +K M+KLIEEDADSFA++AEMYY+KRP L+  VEEFYR YR+LAERYDH T EL  
Sbjct: 35  MDSKVKAMIKLIEEDADSFARRAEMYYKKRPELMKLVEEFYRAYRALAERYDHATVELCH 94

Query: 61  ---------RKNIPSDLQSQGSGISDLGSEPSTTWPSP-------DQRLGRRKSGPRAAG 120
                       +P D+    +  S   SEP T    P       D      K G   + 
Sbjct: 95  AHKTMAEAFPNQVPFDMIEDSA--SSSCSEPRTPEKMPPGIQPFYDSDSATSKRG--LSQ 154

Query: 121 FEFFLGSGGSNSDTCQKEGDESSSLTESEPESDDSSVNNYSGGDQ----------GLNRK 180
              +LG+  +  ++ ++   E  +  E+       S+N +S  ++          GL+ +
Sbjct: 155 LTEYLGNSETEVESLKRTLVELGAEKEALNLQYQLSLNKFSRLEKDLEVAQKDVSGLDER 214

Query: 181 MIELEIE---LREAKEKLRMKEDNA----EGSFPFKGPTDENSDYAFARIAGYEEELRNA 240
             + EIE   L EA  KL  + D A      S       +E+  +A   + G       A
Sbjct: 215 ASKAEIETKILAEALAKLEAERDAALLRYNESMQKITELEESFSHAQEDVKGLTNRATKA 274

Query: 241 NEKLRISDAQITRLKSE----LQKYRQ-----SELTKGLQAESESVSATMEDNQRHEDGV 300
             ++       +RL SE    L +Y +     S L K ++   E+       + + ED +
Sbjct: 275 ETEVENLKQAHSRLHSEKEAGLAEYNRCLEMISNLEKKVRDAEENAQNFSNQSAKAEDEI 334

Query: 301 PLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQKEVSKLKLELENNRSPE 360
             + ++  +V+E   G        +E + + ++      +N+++  S++       ++ E
Sbjct: 335 KALRHELVKVNEVKDGLRLRYQQCLETISKLEREVSHAQDNAKRLSSEVLAGAAKLKTVE 394

Query: 361 -KITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSLSDRDHEIRDLKLSV- 420
            + T L++  E    +      KL+A+ +E+ + Q  + + +  + D      ++++S+ 
Sbjct: 395 DQCTLLESSNETLKLEADGLTHKLAAKDQEIFQKQNELEKFQSLIEDEHSRYLEIEVSLK 454

Query: 421 --------SDAEQKIFPEKAQVKAEMSKLLEERALLME----QVREWEHGARLLEDEIRK 480
                   S  EQK+   + Q +  M + LE R L +E     V+E       L D    
Sbjct: 455 TLQSLYSQSQEEQKVITSELQSRIGMLRDLETRNLKLEGDISSVKEENQNLSELNDSSMI 514

Query: 481 VKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENLSS 540
               +      L    E+LE  +   +      +  +  L+ E D L+     + E ++ 
Sbjct: 515 FLETQKCEISSLKEIKEKLEEEVARHINQSSAFQEEIRRLKDEIDSLNKRYQAIMEQVNL 574

Query: 541 KEKQVDEIRKHVQKLERERVELVS----NIDKADKMAEELRLREKELEGEVEKQRILI-- 555
                  +   V+KL+ E  +L        D  D + E+LR  +  L   V  +++L+  
Sbjct: 575 AGLDPKSLACSVRKLQDENSKLTELCNHQSDDKDALTEKLRELDNILRKNVCLEKLLLES 634

BLAST of Lsi02G012050 vs. ExPASy Swiss-Prot
Match: F4JIF4 (Protein NETWORKED 1B OS=Arabidopsis thaliana OX=3702 GN=NET1B PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.7e-12
Identity = 138/591 (23.35%), Postives = 259/591 (43.82%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MD  +K M+KLIE DADSFA++A+MY++KRP L+  VEE YR YR+LAERYDH T ELR+
Sbjct: 35  MDSKVKTMIKLIEADADSFARRADMYFKKRPELMKLVEELYRAYRALAERYDHTTVELRR 94

Query: 61  -------NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSN 120
                    P+  Q     I D  S  S      D     +K G ++      +      
Sbjct: 95  AHKVMVEAFPN--QMSFDMIEDSASSSSEPRTEADTE-ALQKDGTKSKRSFSQMNKLDGT 154

Query: 121 SDTCQKEGDESS---SLTESEPESD----------------DSSVNNYSGGDQGLNRKMI 180
           SD+ + + +  +   +L E + E +                +  +N+     +G + +  
Sbjct: 155 SDSHEADSEVETLKRTLLELQTEKEALNLQYQLILSKVSRFEKELNDAQKDVKGFDERAC 214

Query: 181 ELEIELREAKEKL-RMKEDNAEGSFPFKGPTDENSDYAFARIAGYEEELRNANEKLRISD 240
           + +IE++  KE L +++ +   G   +    +  +D   A I+  +E  +    ++  ++
Sbjct: 215 KADIEIKILKESLAKLEVERDTGLLQYSQAIERIADLE-ASISHGQEYAKGLTNRVSEAE 274

Query: 241 AQITRLKSELQKYRQSELTKGLQAESESVS--ATMEDNQRHEDGVPLVINQESEVDEHHR 300
            +   LK EL +  QSE   GL   ++S+   +++E   R  +    V   +SE  E   
Sbjct: 275 REAMSLKKELSRL-QSEKEAGLLRYNKSLELISSLEKTIRDAEESVRVFRDQSEQAETEI 334

Query: 301 GSGGDQVITVEGLVEEQKITKERLENSQKEVSKLKLELENNRSPEKITHLQNELEAALKD 360
            +   +++ +  + E+  +   R +   + +SKL+ E+ + +   K   L +E+ A    
Sbjct: 335 KALKQELLKLNEVNEDLNV---RYQQCLETISKLEREVSHAQDNAK--RLSSEVLAGAAK 394

Query: 361 TTTWKAKLS-------AERREVSKLQERISRLKGSLSDRDHEIRDLKLSVSDAEQKIFPE 420
             T + + +         + E   L  ++S     LS + +EI  L+ +V   EQ  F E
Sbjct: 395 IKTVEEQCALLESFNQTMKVEAENLAHKMSAKDQELSQKQNEIEKLQ-AVMQEEQLRFSE 454

Query: 421 KA----QVKAEMSKLLEERALLMEQVREWEHGARLLEDEIRKVKGEKVVLEERLNGEIER 480
                  +++  S+  EE+ +L  ++       R LE    K++G+    EE  N  +  
Sbjct: 455 LGASLRNLESLHSQSQEEQKVLTSELHSRIQMLRELEMRNSKLEGDISSKEE--NRNLSE 514

Query: 481 LETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENLSSKEKQVDEIRKHVQKLERE 540
           +  T +     +E  KN ++ L+  +++L  EV       S+ + ++  ++ ++  + R 
Sbjct: 515 INDTSIS----LEIQKNEISCLKKMKEKLEEEVAKQMNQSSALQVEIHCVKGNIDSMNRR 574

Query: 541 RVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGAEEKREAIRQLC 552
             +L+  +       E L    K+L+ E  K   L     +E      +LC
Sbjct: 575 YQKLIDQVSLTGFDPESLSYSVKKLQDENSKLVELCTNQRDENNAVTGKLC 608

BLAST of Lsi02G012050 vs. ExPASy TrEMBL
Match: A0A5A7UVQ9 (Protein NETWORKED 4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00160 PE=4 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.3e-278
Identity = 525/579 (90.67%), Postives = 548/579 (94.65%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK
Sbjct: 4   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 63

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSE  +TWPSPDQRLGRRKSGPRAAGF+FFLGSGGSNSDTCQKE
Sbjct: 64  NIPSDLQSQGSGISDLGSELPSTWPSPDQRLGRRKSGPRAAGFDFFLGSGGSNSDTCQKE 123

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGS PF
Sbjct: 124 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSLPF 183

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG  DENSDY FARIAGYEEELRNANEKLRISD QI RLKSELQKYR+SE+TKGLQA  E
Sbjct: 184 KGAADENSDYVFARIAGYEEELRNANEKLRISDVQIMRLKSELQKYRESEMTKGLQA--E 243

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           S+SATMED  RHEDGVPLVIN ESEVDEHHRGSG D VITV+ LVEEQKITKERLE SQK
Sbjct: 244 SLSATMEDTLRHEDGVPLVINHESEVDEHHRGSGADHVITVKELVEEQKITKERLETSQK 303

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E+SKLKLELENNRSPEKI HLQNELEAA KDTTTWKAKLSAERREVSKLQERISRLK SL
Sbjct: 304 ELSKLKLELENNRSPEKICHLQNELEAARKDTTTWKAKLSAERREVSKLQERISRLKASL 363

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDRDHEIRDLKL+VSDAEQKIFPEKAQVKA+MSKLLEE+A+LMEQVREWEH ARLLEDEI
Sbjct: 364 SDRDHEIRDLKLAVSDAEQKIFPEKAQVKADMSKLLEEQAVLMEQVREWEHRARLLEDEI 423

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RK+KGEKV LEERLNGEI+RLETTIVEKVE +E  KNGLNDL+SERD+L +EVV LKE L
Sbjct: 424 RKIKGEKVDLEERLNGEIKRLETTIVEKVECMEYFKNGLNDLESERDQLQAEVVALKEKL 483

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
            SK+KQVD+IRKHV KLERERVELVS+IDKADK+AE+LRLREKELEGEVEKQRILIMEGA
Sbjct: 484 RSKDKQVDDIRKHVGKLERERVELVSSIDKADKVAEKLRLREKELEGEVEKQRILIMEGA 543

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIGQKRVPVLAS
Sbjct: 544 EEKREAIRQLCFSIEHYRSGYHMLREVFIGQKRVPVLAS 580

BLAST of Lsi02G012050 vs. ExPASy TrEMBL
Match: A0A1S3C549 (protein NETWORKED 4A OS=Cucumis melo OX=3656 GN=LOC103497012 PE=4 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.3e-278
Identity = 525/579 (90.67%), Postives = 548/579 (94.65%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK
Sbjct: 44  MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSE  +TWPSPDQRLGRRKSGPRAAGF+FFLGSGGSNSDTCQKE
Sbjct: 104 NIPSDLQSQGSGISDLGSELPSTWPSPDQRLGRRKSGPRAAGFDFFLGSGGSNSDTCQKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGS PF
Sbjct: 164 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSLPF 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG  DENSDY FARIAGYEEELRNANEKLRISD QI RLKSELQKYR+SE+TKGLQA  E
Sbjct: 224 KGAADENSDYVFARIAGYEEELRNANEKLRISDVQIMRLKSELQKYRESEMTKGLQA--E 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           S+SATMED  RHEDGVPLVIN ESEVDEHHRGSG D VITV+ LVEEQKITKERLE SQK
Sbjct: 284 SLSATMEDTLRHEDGVPLVINHESEVDEHHRGSGADHVITVKELVEEQKITKERLETSQK 343

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E+SKLKLELENNRSPEKI HLQNELEAA KDTTTWKAKLSAERREVSKLQERISRLK SL
Sbjct: 344 ELSKLKLELENNRSPEKICHLQNELEAARKDTTTWKAKLSAERREVSKLQERISRLKASL 403

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDRDHEIRDLKL+VSDAEQKIFPEKAQVKA+MSKLLEE+A+LMEQVREWEH ARLLEDEI
Sbjct: 404 SDRDHEIRDLKLAVSDAEQKIFPEKAQVKADMSKLLEEQAVLMEQVREWEHRARLLEDEI 463

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RK+KGEKV LEERLNGEI+RLETTIVEKVE +E  KNGLNDL+SERD+L +EVV LKE L
Sbjct: 464 RKIKGEKVDLEERLNGEIKRLETTIVEKVECMEYFKNGLNDLESERDQLQAEVVALKEKL 523

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
            SK+KQVD+IRKHV KLERERVELVS+IDKADK+AE+LRLREKELEGEVEKQRILIMEGA
Sbjct: 524 RSKDKQVDDIRKHVGKLERERVELVSSIDKADKVAEKLRLREKELEGEVEKQRILIMEGA 583

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIGQKRVPVLAS
Sbjct: 584 EEKREAIRQLCFSIEHYRSGYHMLREVFIGQKRVPVLAS 620

BLAST of Lsi02G012050 vs. ExPASy TrEMBL
Match: A0A0A0LSG1 (NAB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G028070 PE=4 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 6.7e-278
Identity = 524/579 (90.50%), Postives = 545/579 (94.13%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK
Sbjct: 44  MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSEP +TWPSPDQRLGRRKSGPRAAGF+FFLGSGGSNSDTCQKE
Sbjct: 104 NIPSDLQSQGSGISDLGSEPPSTWPSPDQRLGRRKSGPRAAGFDFFLGSGGSNSDTCQKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMK DNAEGSFPF
Sbjct: 164 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKADNAEGSFPF 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG  DENSDY FARIAGYEEELRNANEKLRISD QI RLKSELQKYR+S +TKGLQ   E
Sbjct: 224 KGAADENSDYVFARIAGYEEELRNANEKLRISDVQIMRLKSELQKYRESVMTKGLQV--E 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           S+S TME+ QRHEDGVPLVINQESEVDEHHRGSG D  ITVEGLVEEQKITKERLE SQK
Sbjct: 284 SLSDTMEETQRHEDGVPLVINQESEVDEHHRGSGADHAITVEGLVEEQKITKERLEISQK 343

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E+SKLKLELENNRSPEKI HLQNELEAA KDTTTWKAKLSAERREVSKLQERISRLK SL
Sbjct: 344 ELSKLKLELENNRSPEKICHLQNELEAARKDTTTWKAKLSAERREVSKLQERISRLKASL 403

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDRDHEIRDLKL+VSDAEQKIFPEKAQVKAEMSKLLEE+ +LMEQVRE EH ARLLEDEI
Sbjct: 404 SDRDHEIRDLKLAVSDAEQKIFPEKAQVKAEMSKLLEEQTVLMEQVRESEHRARLLEDEI 463

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RK+KGEKV LEERLNGEIERLETTIVEKVE +E  KNGLNDL+SERD+L  E+V LKE L
Sbjct: 464 RKIKGEKVDLEERLNGEIERLETTIVEKVECMEYFKNGLNDLESERDQLQDEIVALKEKL 523

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
            SK+KQVD+IRKHV+KLERERVELVS IDKADK+AE+LRLREKELEGEVEKQRILIMEGA
Sbjct: 524 RSKDKQVDDIRKHVEKLERERVELVSGIDKADKVAEKLRLREKELEGEVEKQRILIMEGA 583

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIGQKRVPVLAS
Sbjct: 584 EEKREAIRQLCFSIEHYRSGYHMLREVFIGQKRVPVLAS 620

BLAST of Lsi02G012050 vs. ExPASy TrEMBL
Match: A0A6J1HMB2 (protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111464876 PE=4 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.5e-253
Identity = 488/579 (84.28%), Postives = 529/579 (91.36%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVE+FYRMYRSLAERYDHVTGELRK
Sbjct: 44  MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEDFYRMYRSLAERYDHVTGELRK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSEPS+TWPSPDQRLGRRKSGPRAAGF+FFLGSGGSNSDTCQKE
Sbjct: 104 NIPSDLQSQGSGISDLGSEPSSTWPSPDQRLGRRKSGPRAAGFDFFLGSGGSNSDTCQKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLT+SEPESDDSSVNNYSGGDQ +NRKMIELEIELR+AKEKLR+KEDNAEGSFPF
Sbjct: 164 GDESSSLTDSEPESDDSSVNNYSGGDQVVNRKMIELEIELRQAKEKLRLKEDNAEGSFPF 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG TDENSD  FAR++ YEEE+RNANEKLRISD QI +LKSELQKYRQ+EL+K LQA  E
Sbjct: 224 KGATDENSDDVFARMSVYEEEVRNANEKLRISDVQIMKLKSELQKYRQAELSKDLQA--E 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           SV AT+ED QRHE+G    INQ+ EV+EH +G G DQVI VEG++EE KITKE+L+NSQK
Sbjct: 284 SVCATVEDIQRHENG---AINQKPEVEEHDKGLGVDQVINVEGVLEELKITKEKLDNSQK 343

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E+SKLKLELENNRSPEKI HLQNELEAA KD TTWKAKLSAERREVSKLQER+SRLK SL
Sbjct: 344 ELSKLKLELENNRSPEKILHLQNELEAARKDATTWKAKLSAERREVSKLQERVSRLKASL 403

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDR+HEIRDLKL+VSDAE KIFPEKAQVKAEMSKL EERA+LMEQVRE EH AR+LEDEI
Sbjct: 404 SDREHEIRDLKLAVSDAELKIFPEKAQVKAEMSKLFEERAVLMEQVREGEHQARVLEDEI 463

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RKVKGEK  LEERL+GEI RLETTIV KVE IEN       L+ ERDELH+EVV+LK+NL
Sbjct: 464 RKVKGEKGDLEERLSGEIGRLETTIVGKVECIEN-------LERERDELHAEVVSLKDNL 523

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
             KEKQVDE+R+HVQKLERERVELVS ++KADK+AEELRLREKELE EVE+QR L+MEGA
Sbjct: 524 RCKEKQVDEVREHVQKLERERVELVSGVEKADKVAEELRLREKELEREVERQRELVMEGA 583

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIG KRVPVLAS
Sbjct: 584 EEKREAIRQLCFSIEHYRSGYHMLREVFIGHKRVPVLAS 610

BLAST of Lsi02G012050 vs. ExPASy TrEMBL
Match: A0A6J1I148 (protein NETWORKED 4A OS=Cucurbita maxima OX=3661 GN=LOC111469564 PE=4 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.8e-252
Identity = 487/579 (84.11%), Postives = 527/579 (91.02%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVE+FYRMYRSLAERYDHVTGELRK
Sbjct: 44  MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEDFYRMYRSLAERYDHVTGELRK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSEPS+TWPSPDQRLGRRKSGPRAAGF+FFLGSGGSNSDTCQKE
Sbjct: 104 NIPSDLQSQGSGISDLGSEPSSTWPSPDQRLGRRKSGPRAAGFDFFLGSGGSNSDTCQKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLT+SEPESDDSSVNNYSGGDQG+NRKMIELEIELR+AKEKLRMKEDNAE SFPF
Sbjct: 164 GDESSSLTDSEPESDDSSVNNYSGGDQGVNRKMIELEIELRQAKEKLRMKEDNAESSFPF 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG TDENSD  FAR++ YEEE+RNANEKLRISD QI +L+SELQKYRQ+EL+K LQA  E
Sbjct: 224 KGATDENSDDVFARMSVYEEEVRNANEKLRISDVQIMKLESELQKYRQAELSKDLQA--E 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           SV ATM D QRHE+G   VINQE E++EH +G G DQVI VEG++EE KI KE+L+NSQK
Sbjct: 284 SVCATMVDVQRHENG---VINQEPEIEEHDKGLGVDQVINVEGVLEELKIMKEKLDNSQK 343

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E+SKLKLELENNRSPEKI HLQNELEAA KDTTTWKAKLSAERREVSKLQER+ RLK SL
Sbjct: 344 ELSKLKLELENNRSPEKILHLQNELEAARKDTTTWKAKLSAERREVSKLQERVLRLKASL 403

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDR+HEIRDLKL+VSDAE KIFPEKAQVKAEMSKL EERA+LMEQVRE EH AR+LEDEI
Sbjct: 404 SDREHEIRDLKLAVSDAELKIFPEKAQVKAEMSKLFEERAVLMEQVREGEHQARVLEDEI 463

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RKVKGEK  LEERL+GEI RLETTIV KVE IEN       L+ ERD+LH+EVV+LK+NL
Sbjct: 464 RKVKGEKGDLEERLSGEIGRLETTIVGKVECIEN-------LERERDKLHAEVVSLKDNL 523

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
             KEKQV EIR+HVQKLERERVELVS ++KADK+AEELRLREKELE EVE+QR L+MEGA
Sbjct: 524 RCKEKQVGEIREHVQKLERERVELVSGVEKADKVAEELRLREKELEREVERQRELVMEGA 583

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIG KRVPVLAS
Sbjct: 584 EEKREAIRQLCFSIEHYRSGYHMLREVFIGHKRVPVLAS 610

BLAST of Lsi02G012050 vs. NCBI nr
Match: XP_038894245.1 (protein NETWORKED 4A isoform X2 [Benincasa hispida])

HSP 1 Score: 969.1 bits (2504), Expect = 1.6e-278
Identity = 528/579 (91.19%), Postives = 552/579 (95.34%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK
Sbjct: 44  MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSEPS+TWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE
Sbjct: 104 NIPSDLQSQGSGISDLGSEPSSTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLTESEPESDDSSVNNYS GDQGLNRKMIELEIELREAKEKLR+KED+AEGSFPF
Sbjct: 164 GDESSSLTESEPESDDSSVNNYSVGDQGLNRKMIELEIELREAKEKLRLKEDSAEGSFPF 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG TDENSDYAFARIAGYEEELRNANEKLRISD QI RLKSELQKYRQSELTKGLQA  E
Sbjct: 224 KGTTDENSDYAFARIAGYEEELRNANEKLRISDKQIMRLKSELQKYRQSELTKGLQA--E 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           S SAT ED QRH D VPLVI+QESEVDEHHR SG DQVIT+E LVEEQKITKERLENSQK
Sbjct: 284 SASATTEDTQRHGDEVPLVIHQESEVDEHHRCSGADQVITIERLVEEQKITKERLENSQK 343

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E++KLKLELEN+RSPEKI HLQNELEAA KDTTTWKAKLSAERREVSKLQERISRLK SL
Sbjct: 344 ELTKLKLELENDRSPEKICHLQNELEAARKDTTTWKAKLSAERREVSKLQERISRLKASL 403

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDRDHEIRDLKL+VSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEH +RLLEDEI
Sbjct: 404 SDRDHEIRDLKLAVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHRSRLLEDEI 463

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RK KGEKV LE+RL+GEIERL T IVEKVE IE LK+GLNDLQSERD+L +EVV+LKENL
Sbjct: 464 RKEKGEKVDLEDRLSGEIERLGTKIVEKVECIEYLKSGLNDLQSERDQLQAEVVSLKENL 523

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
           SSK+KQVDEIRKHVQKLE+ERVELVS IDKA+K+A+ELRLREKELEGEVEKQR++IMEGA
Sbjct: 524 SSKDKQVDEIRKHVQKLEKERVELVSGIDKAEKLAKELRLREKELEGEVEKQRVMIMEGA 583

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIGQKRVPVLAS
Sbjct: 584 EEKREAIRQLCFSIEHYRSGYHMLREVFIGQKRVPVLAS 620

BLAST of Lsi02G012050 vs. NCBI nr
Match: XP_038894244.1 (protein NETWORKED 4A isoform X1 [Benincasa hispida])

HSP 1 Score: 969.1 bits (2504), Expect = 1.6e-278
Identity = 528/579 (91.19%), Postives = 552/579 (95.34%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK
Sbjct: 49  MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 108

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSEPS+TWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE
Sbjct: 109 NIPSDLQSQGSGISDLGSEPSSTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 168

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLTESEPESDDSSVNNYS GDQGLNRKMIELEIELREAKEKLR+KED+AEGSFPF
Sbjct: 169 GDESSSLTESEPESDDSSVNNYSVGDQGLNRKMIELEIELREAKEKLRLKEDSAEGSFPF 228

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG TDENSDYAFARIAGYEEELRNANEKLRISD QI RLKSELQKYRQSELTKGLQA  E
Sbjct: 229 KGTTDENSDYAFARIAGYEEELRNANEKLRISDKQIMRLKSELQKYRQSELTKGLQA--E 288

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           S SAT ED QRH D VPLVI+QESEVDEHHR SG DQVIT+E LVEEQKITKERLENSQK
Sbjct: 289 SASATTEDTQRHGDEVPLVIHQESEVDEHHRCSGADQVITIERLVEEQKITKERLENSQK 348

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E++KLKLELEN+RSPEKI HLQNELEAA KDTTTWKAKLSAERREVSKLQERISRLK SL
Sbjct: 349 ELTKLKLELENDRSPEKICHLQNELEAARKDTTTWKAKLSAERREVSKLQERISRLKASL 408

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDRDHEIRDLKL+VSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEH +RLLEDEI
Sbjct: 409 SDRDHEIRDLKLAVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHRSRLLEDEI 468

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RK KGEKV LE+RL+GEIERL T IVEKVE IE LK+GLNDLQSERD+L +EVV+LKENL
Sbjct: 469 RKEKGEKVDLEDRLSGEIERLGTKIVEKVECIEYLKSGLNDLQSERDQLQAEVVSLKENL 528

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
           SSK+KQVDEIRKHVQKLE+ERVELVS IDKA+K+A+ELRLREKELEGEVEKQR++IMEGA
Sbjct: 529 SSKDKQVDEIRKHVQKLEKERVELVSGIDKAEKLAKELRLREKELEGEVEKQRVMIMEGA 588

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIGQKRVPVLAS
Sbjct: 589 EEKREAIRQLCFSIEHYRSGYHMLREVFIGQKRVPVLAS 625

BLAST of Lsi02G012050 vs. NCBI nr
Match: KAA0059972.1 (protein NETWORKED 4A [Cucumis melo var. makuwa] >TYJ97228.1 protein NETWORKED 4A [Cucumis melo var. makuwa])

HSP 1 Score: 967.6 bits (2500), Expect = 4.7e-278
Identity = 525/579 (90.67%), Postives = 548/579 (94.65%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK
Sbjct: 4   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 63

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSE  +TWPSPDQRLGRRKSGPRAAGF+FFLGSGGSNSDTCQKE
Sbjct: 64  NIPSDLQSQGSGISDLGSELPSTWPSPDQRLGRRKSGPRAAGFDFFLGSGGSNSDTCQKE 123

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGS PF
Sbjct: 124 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSLPF 183

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG  DENSDY FARIAGYEEELRNANEKLRISD QI RLKSELQKYR+SE+TKGLQA  E
Sbjct: 184 KGAADENSDYVFARIAGYEEELRNANEKLRISDVQIMRLKSELQKYRESEMTKGLQA--E 243

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           S+SATMED  RHEDGVPLVIN ESEVDEHHRGSG D VITV+ LVEEQKITKERLE SQK
Sbjct: 244 SLSATMEDTLRHEDGVPLVINHESEVDEHHRGSGADHVITVKELVEEQKITKERLETSQK 303

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E+SKLKLELENNRSPEKI HLQNELEAA KDTTTWKAKLSAERREVSKLQERISRLK SL
Sbjct: 304 ELSKLKLELENNRSPEKICHLQNELEAARKDTTTWKAKLSAERREVSKLQERISRLKASL 363

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDRDHEIRDLKL+VSDAEQKIFPEKAQVKA+MSKLLEE+A+LMEQVREWEH ARLLEDEI
Sbjct: 364 SDRDHEIRDLKLAVSDAEQKIFPEKAQVKADMSKLLEEQAVLMEQVREWEHRARLLEDEI 423

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RK+KGEKV LEERLNGEI+RLETTIVEKVE +E  KNGLNDL+SERD+L +EVV LKE L
Sbjct: 424 RKIKGEKVDLEERLNGEIKRLETTIVEKVECMEYFKNGLNDLESERDQLQAEVVALKEKL 483

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
            SK+KQVD+IRKHV KLERERVELVS+IDKADK+AE+LRLREKELEGEVEKQRILIMEGA
Sbjct: 484 RSKDKQVDDIRKHVGKLERERVELVSSIDKADKVAEKLRLREKELEGEVEKQRILIMEGA 543

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIGQKRVPVLAS
Sbjct: 544 EEKREAIRQLCFSIEHYRSGYHMLREVFIGQKRVPVLAS 580

BLAST of Lsi02G012050 vs. NCBI nr
Match: XP_008457286.1 (PREDICTED: protein NETWORKED 4A [Cucumis melo])

HSP 1 Score: 967.6 bits (2500), Expect = 4.7e-278
Identity = 525/579 (90.67%), Postives = 548/579 (94.65%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK
Sbjct: 44  MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSE  +TWPSPDQRLGRRKSGPRAAGF+FFLGSGGSNSDTCQKE
Sbjct: 104 NIPSDLQSQGSGISDLGSELPSTWPSPDQRLGRRKSGPRAAGFDFFLGSGGSNSDTCQKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGS PF
Sbjct: 164 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSLPF 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG  DENSDY FARIAGYEEELRNANEKLRISD QI RLKSELQKYR+SE+TKGLQA  E
Sbjct: 224 KGAADENSDYVFARIAGYEEELRNANEKLRISDVQIMRLKSELQKYRESEMTKGLQA--E 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           S+SATMED  RHEDGVPLVIN ESEVDEHHRGSG D VITV+ LVEEQKITKERLE SQK
Sbjct: 284 SLSATMEDTLRHEDGVPLVINHESEVDEHHRGSGADHVITVKELVEEQKITKERLETSQK 343

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E+SKLKLELENNRSPEKI HLQNELEAA KDTTTWKAKLSAERREVSKLQERISRLK SL
Sbjct: 344 ELSKLKLELENNRSPEKICHLQNELEAARKDTTTWKAKLSAERREVSKLQERISRLKASL 403

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDRDHEIRDLKL+VSDAEQKIFPEKAQVKA+MSKLLEE+A+LMEQVREWEH ARLLEDEI
Sbjct: 404 SDRDHEIRDLKLAVSDAEQKIFPEKAQVKADMSKLLEEQAVLMEQVREWEHRARLLEDEI 463

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RK+KGEKV LEERLNGEI+RLETTIVEKVE +E  KNGLNDL+SERD+L +EVV LKE L
Sbjct: 464 RKIKGEKVDLEERLNGEIKRLETTIVEKVECMEYFKNGLNDLESERDQLQAEVVALKEKL 523

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
            SK+KQVD+IRKHV KLERERVELVS+IDKADK+AE+LRLREKELEGEVEKQRILIMEGA
Sbjct: 524 RSKDKQVDDIRKHVGKLERERVELVSSIDKADKVAEKLRLREKELEGEVEKQRILIMEGA 583

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIGQKRVPVLAS
Sbjct: 584 EEKREAIRQLCFSIEHYRSGYHMLREVFIGQKRVPVLAS 620

BLAST of Lsi02G012050 vs. NCBI nr
Match: KGN63929.1 (hypothetical protein Csa_014034 [Cucumis sativus])

HSP 1 Score: 966.1 bits (2496), Expect = 1.4e-277
Identity = 524/579 (90.50%), Postives = 545/579 (94.13%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK
Sbjct: 44  MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           NIPSDLQSQGSGISDLGSEP +TWPSPDQRLGRRKSGPRAAGF+FFLGSGGSNSDTCQKE
Sbjct: 104 NIPSDLQSQGSGISDLGSEPPSTWPSPDQRLGRRKSGPRAAGFDFFLGSGGSNSDTCQKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
           GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMK DNAEGSFPF
Sbjct: 164 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKADNAEGSFPF 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
           KG  DENSDY FARIAGYEEELRNANEKLRISD QI RLKSELQKYR+S +TKGLQ   E
Sbjct: 224 KGAADENSDYVFARIAGYEEELRNANEKLRISDVQIMRLKSELQKYRESVMTKGLQV--E 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           S+S TME+ QRHEDGVPLVINQESEVDEHHRGSG D  ITVEGLVEEQKITKERLE SQK
Sbjct: 284 SLSDTMEETQRHEDGVPLVINQESEVDEHHRGSGADHAITVEGLVEEQKITKERLEISQK 343

Query: 301 EVSKLKLELENNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGSL 360
           E+SKLKLELENNRSPEKI HLQNELEAA KDTTTWKAKLSAERREVSKLQERISRLK SL
Sbjct: 344 ELSKLKLELENNRSPEKICHLQNELEAARKDTTTWKAKLSAERREVSKLQERISRLKASL 403

Query: 361 SDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDEI 420
           SDRDHEIRDLKL+VSDAEQKIFPEKAQVKAEMSKLLEE+ +LMEQVRE EH ARLLEDEI
Sbjct: 404 SDRDHEIRDLKLAVSDAEQKIFPEKAQVKAEMSKLLEEQTVLMEQVRESEHRARLLEDEI 463

Query: 421 RKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKENL 480
           RK+KGEKV LEERLNGEIERLETTIVEKVE +E  KNGLNDL+SERD+L  E+V LKE L
Sbjct: 464 RKIKGEKVDLEERLNGEIERLETTIVEKVECMEYFKNGLNDLESERDQLQDEIVALKEKL 523

Query: 481 SSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEGA 540
            SK+KQVD+IRKHV+KLERERVELVS IDKADK+AE+LRLREKELEGEVEKQRILIMEGA
Sbjct: 524 RSKDKQVDDIRKHVEKLERERVELVSGIDKADKVAEKLRLREKELEGEVEKQRILIMEGA 583

Query: 541 EEKREAIRQLCLSIDHYRSGYHMLREVFIGQKRVPVLAS 580
           EEKREAIRQLC SI+HYRSGYHMLREVFIGQKRVPVLAS
Sbjct: 584 EEKREAIRQLCFSIEHYRSGYHMLREVFIGQKRVPVLAS 620

BLAST of Lsi02G012050 vs. TAIR 10
Match: AT5G58320.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 389.4 bits (999), Expect = 5.0e-108
Identity = 270/580 (46.55%), Postives = 353/580 (60.86%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRS+KRM+KLIEEDADSFAKKAEMYYQ RP LI+ V+EF+RMYR+LAERY+++TGELRK
Sbjct: 44  MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 103

Query: 61  NIPSDLQSQGSGISDL-GSEPSTTWPSPD-QRLGRRKSGPRAAGFEFFLGSGGSNSDTCQ 120
             P +LQSQGSG+SD+  S+ S  W S +  RLGR  SG RA GFE+FLG+GG  SD   
Sbjct: 104 GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 163

Query: 121 KEGDESSSLTESEPESDDSSVNNYSG------GDQGLNRKMIELEIELREAKEKLRMK-E 180
           K+GD+S+S+T+SE ESDDSSV NY G        Q L++++++LEIELREAKE+LRM+ E
Sbjct: 164 KDGDDSASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRMQLE 223

Query: 181 DNAEGSFPFKGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSEL 240
            N E   P      +  D+  A++A  E+EL++ NEKL+ S+ QI  LKS+L +Y    L
Sbjct: 224 GNTESLLPRVKSETKFVDFP-AKLAACEQELKDVNEKLQNSEDQIYILKSQLARY----L 283

Query: 241 TKGLQAESESVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKIT 300
             GL                               DE   G+   Q + +E L EE +IT
Sbjct: 284 PSGLD------------------------------DEQSEGAASTQELDIETLSEELRIT 343

Query: 301 KERLENSQKEVSKLKLELENNRSPE-KITHLQNELEAALKDTTTWKAKLSAERREVSKLQ 360
             RL  ++K+   ++ E+E ++S + K+  LQ+ LE+A K+   WK+K SA++REV KL 
Sbjct: 344 SLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQDMLESAQKEAAAWKSKASADKREVVKLL 403

Query: 361 ERISRLKGSLSDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWE 420
           +RIS LK SL+ RDHEIRDLK ++SDAE+KIFPEKAQVKA+++KLLEE+    +Q +E E
Sbjct: 404 DRISMLKSSLAGRDHEIRDLKTALSDAEEKIFPEKAQVKADIAKLLEEKIHRDDQFKELE 463

Query: 421 HGARLLEDEIRKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELH 480
              R LEDE RKV  EK+  EE+L  EIE L    VEK   IE L   +++L+SE   L 
Sbjct: 464 ANVRYLEDERRKVNNEKIEEEEKLKSEIEVLTLEKVEKGRCIETLSRKVSELESEISRLG 523

Query: 481 SEVVTLKENLSSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVE 540
           SE+           K  D+                               R  E+E EVE
Sbjct: 524 SEI-----------KARDD-------------------------------RTMEMEKEVE 546

Query: 541 KQRILIMEGAEEKREAIRQLCLSIDHYRSGYHMLREVFIG 571
           KQR  + E AEEKRE IRQLC S+D+ R  Y  LR  F G
Sbjct: 584 KQRRELEEVAEEKREVIRQLCFSLDYSRDEYKRLRIAFSG 546

BLAST of Lsi02G012050 vs. TAIR 10
Match: AT5G58320.1 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 361.7 bits (927), Expect = 1.1e-99
Identity = 237/479 (49.48%), Postives = 314/479 (65.55%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRS+KRM+KLIEEDADSFAKKAEMYYQ RP LI+ V+EF+RMYR+LAERY+++TGELRK
Sbjct: 44  MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 103

Query: 61  NIPSDLQSQGSGISDL-GSEPSTTWPSPD-QRLGRRKSGPRAAGFEFFLGSGGSNSDTCQ 120
             P +LQSQGSG+SD+  S+ S  W S +  RLGR  SG RA GFE+FLG+GG  SD   
Sbjct: 104 GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 163

Query: 121 KEGDESSSLTESEPESDDSSVNNYSG------GDQGLNRKMIELEIELREAKEKLRMK-E 180
           K+GD+S+S+T+SE ESDDSSV NY G        Q L++++++LEIELREAKE+LRM+ E
Sbjct: 164 KDGDDSASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRMQLE 223

Query: 181 DNAEGSFPFKGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSEL 240
            N E   P      +  D+  A++A  E+EL++ NEKL+ S+ QI  LKS+L +Y    L
Sbjct: 224 GNTESLLPRVKSETKFVDFP-AKLAACEQELKDVNEKLQNSEDQIYILKSQLARY----L 283

Query: 241 TKGLQAESESVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKIT 300
             GL                               DE   G+   Q + +E L EE +IT
Sbjct: 284 PSGLD------------------------------DEQSEGAASTQELDIETLSEELRIT 343

Query: 301 KERLENSQKEVSKLKLELENNRSPE-KITHLQNELEAALKDTTTWKAKLSAERREVSKLQ 360
             RL  ++K+   ++ E+E ++S + K+  LQ+ LE+A K+   WK+K SA++REV KL 
Sbjct: 344 SLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQDMLESAQKEAAAWKSKASADKREVVKLL 403

Query: 361 ERISRLKGSLSDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWE 420
           +RIS LK SL+ RDHEIRDLK ++SDAE+KIFPEKAQVKA+++KLLEE+    +Q +E E
Sbjct: 404 DRISMLKSSLAGRDHEIRDLKTALSDAEEKIFPEKAQVKADIAKLLEEKIHRDDQFKELE 463

Query: 421 HGARLLEDEIRKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDEL 470
              R LEDE RKV  EK+  EE+L  EIE L    VEK   IE L   +++L+SE   L
Sbjct: 464 ANVRYLEDERRKVNNEKIEEEEKLKSEIEVLTLEKVEKGRCIETLSRKVSELESEISRL 487

BLAST of Lsi02G012050 vs. TAIR 10
Match: AT5G58320.3 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 361.7 bits (927), Expect = 1.1e-99
Identity = 237/479 (49.48%), Postives = 314/479 (65.55%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MDRS+KRM+KLIEEDADSFAKKAEMYYQ RP LI+ V+EF+RMYR+LAERY+++TGELRK
Sbjct: 1   MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELIALVDEFHRMYRALAERYENITGELRK 60

Query: 61  NIPSDLQSQGSGISDL-GSEPSTTWPSPD-QRLGRRKSGPRAAGFEFFLGSGGSNSDTCQ 120
             P +LQSQGSG+SD+  S+ S  W S +  RLGR  SG RA GFE+FLG+GG  SD   
Sbjct: 61  GSPLELQSQGSGLSDISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYH 120

Query: 121 KEGDESSSLTESEPESDDSSVNNYSG------GDQGLNRKMIELEIELREAKEKLRMK-E 180
           K+GD+S+S+T+SE ESDDSSV NY G        Q L++++++LEIELREAKE+LRM+ E
Sbjct: 121 KDGDDSASITDSELESDDSSVTNYPGYVSIGSDFQSLSKRIMDLEIELREAKERLRMQLE 180

Query: 181 DNAEGSFPFKGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSEL 240
            N E   P      +  D+  A++A  E+EL++ NEKL+ S+ QI  LKS+L +Y    L
Sbjct: 181 GNTESLLPRVKSETKFVDFP-AKLAACEQELKDVNEKLQNSEDQIYILKSQLARY----L 240

Query: 241 TKGLQAESESVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKIT 300
             GL                               DE   G+   Q + +E L EE +IT
Sbjct: 241 PSGLD------------------------------DEQSEGAASTQELDIETLSEELRIT 300

Query: 301 KERLENSQKEVSKLKLELENNRSPE-KITHLQNELEAALKDTTTWKAKLSAERREVSKLQ 360
             RL  ++K+   ++ E+E ++S + K+  LQ+ LE+A K+   WK+K SA++REV KL 
Sbjct: 301 SLRLREAEKQNGIMRKEVEKSKSDDAKLKSLQDMLESAQKEAAAWKSKASADKREVVKLL 360

Query: 361 ERISRLKGSLSDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWE 420
           +RIS LK SL+ RDHEIRDLK ++SDAE+KIFPEKAQVKA+++KLLEE+    +Q +E E
Sbjct: 361 DRISMLKSSLAGRDHEIRDLKTALSDAEEKIFPEKAQVKADIAKLLEEKIHRDDQFKELE 420

Query: 421 HGARLLEDEIRKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDEL 470
              R LEDE RKV  EK+  EE+L  EIE L    VEK   IE L   +++L+SE   L
Sbjct: 421 ANVRYLEDERRKVNNEKIEEEEKLKSEIEVLTLEKVEKGRCIETLSRKVSELESEISRL 444

BLAST of Lsi02G012050 vs. TAIR 10
Match: AT2G30500.1 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 328.9 bits (842), Expect = 8.0e-90
Identity = 247/582 (42.44%), Postives = 332/582 (57.04%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MD  +  MLKLIEEDADSFAKKA+MY+QKRP LI  VEEFYRMYR+LAERYD  +GEL+K
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           N  S++QSQ S   ++ S          ++L RR+S                      KE
Sbjct: 104 NHTSEIQSQSS--LEISSPTK-------EKLSRRQSS--------------------HKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
            ++SSSLT+S  +SD SS N+   GD+ L R+M ELE+EL+E K+KL +++++ +G    
Sbjct: 164 EEDSSSLTDSGSDSDHSSAND-EDGDEALIRRMAELELELQETKQKLLLQQESVDG---- 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
               D N D    +I  YE EL+ ANEK+R+ + +I  LK++LQ +   +    L AE +
Sbjct: 224 ----DNNVD-LLHKITTYEGELKEANEKMRMHEDEIANLKNQLQSFMSFDTEDHLGAEQK 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           SV    ED +  ED V                        V  L EE  I KE+L++ +K
Sbjct: 284 SVDLDKEDTK--EDAV---------------------ATKVLALEEELSIAKEKLQHFEK 343

Query: 301 EVSKLKLELE-NNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGS 360
           E   LK ELE    + EK+  LQ+ELE A +D  T+  KL+AE++EV KLQER++ +K S
Sbjct: 344 ETYSLKNELEIGKAAEEKLKSLQHELELAQRDADTYINKLNAEKKEVLKLQERLAMVKTS 403

Query: 361 LSDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDE 420
           L DRD+EIR LK +VSDAEQKIFPEKAQ+K EMSK+LEER+ L EQ+RE       LE  
Sbjct: 404 LQDRDNEIRALKTAVSDAEQKIFPEKAQIKGEMSKMLEERSQLGEQLRE-------LESH 463

Query: 421 IRKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKEN 480
           IR +K EK   EE+L G  E++               +G+ D          E   L+E 
Sbjct: 464 IRLIKEEKAETEEKLRGGTEKI---------------SGMRD----------ESNVLREE 517

Query: 481 LSSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEG 540
           +  +E+++ E  KH+++L  E+V               LR R  EL  EVE+ R+   E 
Sbjct: 524 IGKREEKIKETEKHMEELHMEQV--------------RLRRRSSELTEEVERTRVSASEM 517

Query: 541 AEEKREAIRQLCLSIDHYRSGYHMLREVFIG--QKRVPVLAS 580
           AE+KREAIRQLC+S+DHYR GY  L  V  G   KRV VL++
Sbjct: 584 AEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVVVLST 517

BLAST of Lsi02G012050 vs. TAIR 10
Match: AT2G30500.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 328.9 bits (842), Expect = 8.0e-90
Identity = 247/582 (42.44%), Postives = 332/582 (57.04%), Query Frame = 0

Query: 1   MDRSIKRMLKLIEEDADSFAKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRK 60
           MD  +  MLKLIEEDADSFAKKA+MY+QKRP LI  VEEFYRMYR+LAERYD  +GEL+K
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEFYRMYRALAERYDQASGELQK 103

Query: 61  NIPSDLQSQGSGISDLGSEPSTTWPSPDQRLGRRKSGPRAAGFEFFLGSGGSNSDTCQKE 120
           N  S++QSQ S   ++ S          ++L RR+S                      KE
Sbjct: 104 NHTSEIQSQSS--LEISSPTK-------EKLSRRQSS--------------------HKE 163

Query: 121 GDESSSLTESEPESDDSSVNNYSGGDQGLNRKMIELEIELREAKEKLRMKEDNAEGSFPF 180
            ++SSSLT+S  +SD SS N+   GD+ L R+M ELE+EL+E K+KL +++++ +G    
Sbjct: 164 EEDSSSLTDSGSDSDHSSAND-EDGDEALIRRMAELELELQETKQKLLLQQESVDG---- 223

Query: 181 KGPTDENSDYAFARIAGYEEELRNANEKLRISDAQITRLKSELQKYRQSELTKGLQAESE 240
               D N D    +I  YE EL+ ANEK+R+ + +I  LK++LQ +   +    L AE +
Sbjct: 224 ----DNNVD-LLHKITTYEGELKEANEKMRMHEDEIANLKNQLQSFMSFDTEDHLGAEQK 283

Query: 241 SVSATMEDNQRHEDGVPLVINQESEVDEHHRGSGGDQVITVEGLVEEQKITKERLENSQK 300
           SV    ED +  ED V                        V  L EE  I KE+L++ +K
Sbjct: 284 SVDLDKEDTK--EDAV---------------------ATKVLALEEELSIAKEKLQHFEK 343

Query: 301 EVSKLKLELE-NNRSPEKITHLQNELEAALKDTTTWKAKLSAERREVSKLQERISRLKGS 360
           E   LK ELE    + EK+  LQ+ELE A +D  T+  KL+AE++EV KLQER++ +K S
Sbjct: 344 ETYSLKNELEIGKAAEEKLKSLQHELELAQRDADTYINKLNAEKKEVLKLQERLAMVKTS 403

Query: 361 LSDRDHEIRDLKLSVSDAEQKIFPEKAQVKAEMSKLLEERALLMEQVREWEHGARLLEDE 420
           L DRD+EIR LK +VSDAEQKIFPEKAQ+K EMSK+LEER+ L EQ+RE       LE  
Sbjct: 404 LQDRDNEIRALKTAVSDAEQKIFPEKAQIKGEMSKMLEERSQLGEQLRE-------LESH 463

Query: 421 IRKVKGEKVVLEERLNGEIERLETTIVEKVEYIENLKNGLNDLQSERDELHSEVVTLKEN 480
           IR +K EK   EE+L G  E++               +G+ D          E   L+E 
Sbjct: 464 IRLIKEEKAETEEKLRGGTEKI---------------SGMRD----------ESNVLREE 517

Query: 481 LSSKEKQVDEIRKHVQKLERERVELVSNIDKADKMAEELRLREKELEGEVEKQRILIMEG 540
           +  +E+++ E  KH+++L  E+V               LR R  EL  EVE+ R+   E 
Sbjct: 524 IGKREEKIKETEKHMEELHMEQV--------------RLRRRSSELTEEVERTRVSASEM 517

Query: 541 AEEKREAIRQLCLSIDHYRSGYHMLREVFIG--QKRVPVLAS 580
           AE+KREAIRQLC+S+DHYR GY  L  V  G   KRV VL++
Sbjct: 584 AEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVVVLST 517

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4KEW87.0e-10746.55Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1[more]
Q84VY21.1e-8842.44Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1[more]
F4HZB54.8e-1524.25Protein NETWORKED 1D OS=Arabidopsis thaliana OX=3702 GN=NET1D PE=3 SV=1[more]
Q9LUI22.0e-1323.83Protein NETWORKED 1A OS=Arabidopsis thaliana OX=3702 GN=NET1A PE=1 SV=1[more]
F4JIF41.7e-1223.35Protein NETWORKED 1B OS=Arabidopsis thaliana OX=3702 GN=NET1B PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UVQ92.3e-27890.67Protein NETWORKED 4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194... [more]
A0A1S3C5492.3e-27890.67protein NETWORKED 4A OS=Cucumis melo OX=3656 GN=LOC103497012 PE=4 SV=1[more]
A0A0A0LSG16.7e-27890.50NAB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G028070 PE=4 SV... [more]
A0A6J1HMB21.5e-25384.28protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111464876 PE=4 SV=... [more]
A0A6J1I1482.8e-25284.11protein NETWORKED 4A OS=Cucurbita maxima OX=3661 GN=LOC111469564 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038894245.11.6e-27891.19protein NETWORKED 4A isoform X2 [Benincasa hispida][more]
XP_038894244.11.6e-27891.19protein NETWORKED 4A isoform X1 [Benincasa hispida][more]
KAA0059972.14.7e-27890.67protein NETWORKED 4A [Cucumis melo var. makuwa] >TYJ97228.1 protein NETWORKED 4A... [more]
XP_008457286.14.7e-27890.67PREDICTED: protein NETWORKED 4A [Cucumis melo][more]
KGN63929.11.4e-27790.50hypothetical protein Csa_014034 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G58320.25.0e-10846.55Kinase interacting (KIP1-like) family protein [more]
AT5G58320.11.1e-9949.48Kinase interacting (KIP1-like) family protein [more]
AT5G58320.31.1e-9949.48Kinase interacting (KIP1-like) family protein [more]
AT2G30500.18.0e-9042.44Kinase interacting (KIP1-like) family protein [more]
AT2G30500.28.0e-9042.44Kinase interacting (KIP1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 195..229
NoneNo IPR availableCOILSCoilCoilcoord: 385..405
NoneNo IPR availableCOILSCoilCoilcoord: 413..433
NoneNo IPR availableCOILSCoilCoilcoord: 146..173
NoneNo IPR availableCOILSCoilCoilcoord: 339..359
NoneNo IPR availableCOILSCoilCoilcoord: 452..535
NoneNo IPR availableCOILSCoilCoilcoord: 281..312
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..148
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..147
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..251
NoneNo IPR availablePANTHERPTHR32258:SF21KINASE INTERACTING (KIP1-LIKE) FAMILY PROTEINcoord: 507..579
NoneNo IPR availablePANTHERPTHR32258PROTEIN NETWORKED 4Acoord: 1..509
coord: 507..579
NoneNo IPR availablePANTHERPTHR32258:SF21KINASE INTERACTING (KIP1-LIKE) FAMILY PROTEINcoord: 1..509
IPR011684Protein Networked (NET), actin-binding (NAB) domainPFAMPF07765KIP1coord: 1..52
e-value: 9.0E-23
score: 80.2
IPR011684Protein Networked (NET), actin-binding (NAB) domainPROSITEPS51774NABcoord: 1..58
score: 38.012196

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G012050.1Lsi02G012050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0003779 actin binding