Lsi02G010830 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G010830
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionUbiquinol oxidase
Locationchr02: 12159738 .. 12163547 (-)
RNA-Seq ExpressionLsi02G010830
SyntenyLsi02G010830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGAAATGGCCTCGGGAAAGAGCCAGCTGCCGATTGACAATTCCTCCGCGGTTCATCCTTCTCATACTTCCCCCCACATTCTCTTCTTCTTCCTCCATTCTAACCTTCATTTCCCAAGCTATGGCTACTGCTTCCTCTTCTTCCTCTTCCGCAGTTTTCGCTATCTCTTCCCTCCAATTGAAGCCAAACAACGTAGCCTCTTACAGAATCCCCAATTCCTTCTCTCGCCCTTCCTTCTTTGCACGCCCTTTAATTTCCAGGTAACACTTCCTCTAAATGCTTCTTTAATACCTACACCTTTTGGATTTTATCTGCTTCCTTCATTGCCATTTCTTGAATGGTTTTTGATTTCATATTATTCGCAATTTCATTTTCTTGATGTAGAAAATCATGTCGGGTGCTGGCAACAGTTGTTAAGGAAAATGAGGAAAAAGTGGTTGTGGAGGATTCATTCCCTCCTAAGGCTTCTCCTGTCCACGACGGGGAAGGAGGCAGTAACCAAGGACCAGAAAATTCATCCTCCAATGGCTTGGATAAATGGATCATTAAGCTTGAACAATCTGTTAATGTTCTTCTCACGGTAATCTCAGTTCAAACTGATCATTGCTCTTCATTTGTGCAAAATTGGTTCTCTAATACTTGATATGTTGAATCGTGAACTCTGCTTTACTAATCTTTTCAGGATTCAGTAATAAGGATTCTTGATGCATTTTACCACGATAGACACTACGCAAGGTTCTTTGTCTTGGAAACTATTGCAAGGGTTCCCTATTTTGGTGAGATTTTTTTTTTTCTTCCTTGAAACAGAGCTAACCTGTAATAATCTTAATATGAGTGGGACTTTTATTTTGGTGAAGTTTGGAAGGCTGTAGAAACGATTTTATGACGCCTAATGCTTATGAAAAGCATTTTCATTCAGTGAAATTGAGTTATCTAATAAATATGCAGATAGTTGTTTATTTCAAAGAATTGATTCTGCAACTAACTAATTCTGGAGCCAGATTGTACATCTTCTGTGGATGTTATTGAGGATTAGAACAGTAGTATCTAGTTTGAAATTGTTTCTAATTTCTCCCTTTTAAATTGCAGCGTTTCTGTCAGTTCTGCATTTGTATGAAAGTTTTGGCTGGTGGAGAAGAGCTGATTATCTTAAAGTTCACTTTGCTGAGAGCTGGAATGAGATGCATCACTTGCTGATCATGGAGGTACTTATTATCTTGGGCTTCCTTTAGATGGCACATAATATCCTTTCCTTAAGTTGCCCCATTGGAAACTCCATGATTTCATCCTGGTTTTAGTTGGAAGTATTTCCTTTTTTCATTTGTCCTAGTATGTTGTAGGACTATGATAGGAATATTATTAGGAAATAATTAGAGTATTAAGAGCATATTAGCAATTAGTTAAGGAGTATCAAGTATGTTAGGGAAGTTTGGTTATAAATAGAGTGAGTAGGGAAGGAGATCTTGAGATTGAGAGGGTCCAGGTACTTCGAATTACTTGGTATATCTTGTAGTTTAGTTACCATTTATATTCCAATATATTTGAGTTCTACTAGACTATATTGATCATAATGGTGGCTGCATAAGACTTTCCTATTTCCTACAATATATTTTCCTTTTGAATCATCCATGTTTAACCCTGTGCACCCATCTAGACATTGAATTACTGAATAAATTATCTCTGTTTCTGACTTGAGTGCAGGAATTGGGGGGTAATGATGGGTGGTTTGATCGATTTCTTGCTCAACATATCGCTGTTGCTTATTATTTTATGACAGTACTGATGTACATGATTAGCCCAAGAATGGCATGTAAGTATTGTTGGAGCTGATAAATTCAACCTGCTTATACTCTCTTTCTTGCATCCTTTTATGGGCTTGTTCAGTTTTTACAACTGTACTAAGTTACCAAGAAAACAGAAAATTAGCATGACTTTCCTTCTCAATAAAACTTACAAAGGAACTAAAATGTAAATTGCAGTTTGGTGTAAAACAATAAGAAGTGGCGAGTTGCTTTGGGACACCTTTCTACTTTGCTGCGCTTTAACATCTTAAGTTCTTTGCATTCAACTTTTAGTGACATTGTTCTCTATTGATATTTGATTAGTGCAGATCATCTTTCTGAATGTGTTGAAAGCCATGCATTTTCAACTTATGATAAGTTTATAAAGGCCCAAGGAGGTACGTTGGTTGCATTATTTGATGATGATCTTAAATTTCATCTAGTTAACATTAATTCTCGAAGGGGAATATCGTTCTGACGTGTTCCATTCCTTGTGCTATAGAGGAGTTGAAAAAATTGCCTGCTCCCGAGGTTGCTGTGAAGTATTACACTGAGGGAGACTTGTACTTATTTGGTAAGTTCTAGCTTTCTTACCACGTGCACAGTTATTTCGTTCAAACTGACACTAACAGATTGATTTCATTCAGATGAATTTCAAACTTCCAGAGCTCCAAAATCACGAAGACCAAAAATAGGTAAATTTTATAATATGGAATAGTTACCGTACTTCATCGCCCAATATATAATACTACCATCATGAAGGCATGTCTTAAAAATAGAAGGATAAGAGTAGAAGCCCCAAGCACGCGTATGTGACTCTTTTTCTCTTCTTTTTCTTTGAAGTTTTAAGATCCCTCAAACTAGATAGACCTTTAAACATTACTGGCTGATAAATCATAATATTATACGTAATAGTTGATATTTGTGGTAATTGGTTCGTTGTATCTGCGTTCTTTGTGTTGGAAAGTTCATTTTACTAGATATAAATTTATATGCACCTGAGAAATTGATTAATGAAGGAAATAGTTGGAGTATTAAAAGTTTGATACCTTCATGGCTTCATTTCCAGAATTGACCTTAAAATTAGACTTGATTGTGAAACATCAAAAGTTTTACAACTCTTCAATTGTAAAAAAAAGGTTTTGATTCCCTTTTTTCTGCACCAACTTGATCTCCCACTTCTTCCTTTGCTTTTCTTCAATATACACAGTTTTCGTTAGGCAAAGTTGCAAGATTGCTAGGAGTGCAAACAAAATCCCACATTGGTTAAGGAATGGAATGATTATGGGTATATAAGTGAGGTCAATTATCTTTATTGGTATGAGACTTTTTGGGTGGTACCAAAAGCAAAGTCATGAAGCTTAAAAAGAGGGTGTATTGGCTTTGTCTAGCAAGGCAACACATGACCTTACTTGAACAGAATCTGTTGAAATATGCTATAATTGTATCTCAGATCAGTTGCTTGATTGACTTCAAGCATTCAGAAATTTTCTCAAAAGAAACTGTATAATACATGACAGCACTTAAGAACTGTTATATATTTGTTTTCAATTGTCGCCATATTTCTAATTCTTGCAAGTTTGAATATGATACTTTTTTTTCGTAGAGAGCTTGTACGATGTGTTTGTGAACATCAGAGACGACGAAGCCGAGCATTGCAAGACCATGAGAGCTTGTCAATCGCATGGGAACCTCCGGTCACCACATTCTTATTCAGAGAGCTATCCTGAAGATGATGAGCCACTCTGCAACCTTGCTGAAGCAGATTGTGAAGGCATTGTAGACTGTATAAAGAAATCCGTTACTTCTTCCCCTCCGGTAAAGCAAAAACTATGATATGCGTATATACATATAAAGAAAATGTAAGTTCACTAAATTCACACGTAGTAAATATCTGTCACATACATACAGGAGAAAAAATGTAAGGTCCATATATAGGTTATTTGATGATCTTACAAAAGCTGACATTGCACCTATCTTTGTAATGGACTGCTTATACCAATAATATAGAGTTCAAAATTCTGATGTC

mRNA sequence

GGGAAATGGCCTCGGGAAAGAGCCAGCTGCCGATTGACAATTCCTCCGCGGTTCATCCTTCTCATACTTCCCCCCACATTCTCTTCTTCTTCCTCCATTCTAACCTTCATTTCCCAAGCTATGGCTACTGCTTCCTCTTCTTCCTCTTCCGCAGTTTTCGCTATCTCTTCCCTCCAATTGAAGCCAAACAACGTAGCCTCTTACAGAATCCCCAATTCCTTCTCTCGCCCTTCCTTCTTTGCACGCCCTTTAATTTCCAGAAAATCATGTCGGGTGCTGGCAACAGTTGTTAAGGAAAATGAGGAAAAAGTGGTTGTGGAGGATTCATTCCCTCCTAAGGCTTCTCCTGTCCACGACGGGGAAGGAGGCAGTAACCAAGGACCAGAAAATTCATCCTCCAATGGCTTGGATAAATGGATCATTAAGCTTGAACAATCTGTTAATGTTCTTCTCACGGATTCAGTAATAAGGATTCTTGATGCATTTTACCACGATAGACACTACGCAAGGTTCTTTGTCTTGGAAACTATTGCAAGGGTTCCCTATTTTGCGTTTCTGTCAGTTCTGCATTTGTATGAAAGTTTTGGCTGGTGGAGAAGAGCTGATTATCTTAAAGTTCACTTTGCTGAGAGCTGGAATGAGATGCATCACTTGCTGATCATGGAGGAATTGGGGGGTAATGATGGGTGGTTTGATCGATTTCTTGCTCAACATATCGCTGTTGCTTATTATTTTATGACAGTACTGATGTACATGATTAGCCCAAGAATGGCATATCATCTTTCTGAATGTGTTGAAAGCCATGCATTTTCAACTTATGATAAGTTTATAAAGGCCCAAGGAGAGGAGTTGAAAAAATTGCCTGCTCCCGAGGTTGCTGTGAAGTATTACACTGAGGGAGACTTGTACTTATTTGAGAGCTTGTACGATGTGTTTGTGAACATCAGAGACGACGAAGCCGAGCATTGCAAGACCATGAGAGCTTGTCAATCGCATGGGAACCTCCGGTCACCACATTCTTATTCAGAGAGCTATCCTGAAGATGATGAGCCACTCTGCAACCTTGCTGAAGCAGATTGTGAAGGCATTGTAGACTGTATAAAGAAATCCGTTACTTCTTCCCCTCCGGTAAAGCAAAAACTATGATATGCGTATATACATATAAAGAAAATGTAAGTTCACTAAATTCACACGTAGTAAATATCTGTCACATACATACAGGAGAAAAAATGTAAGGTCCATATATAGGTTATTTGATGATCTTACAAAAGCTGACATTGCACCTATCTTTGTAATGGACTGCTTATACCAATAATATAGAGTTCAAAATTCTGATGTC

Coding sequence (CDS)

ATGGCTACTGCTTCCTCTTCTTCCTCTTCCGCAGTTTTCGCTATCTCTTCCCTCCAATTGAAGCCAAACAACGTAGCCTCTTACAGAATCCCCAATTCCTTCTCTCGCCCTTCCTTCTTTGCACGCCCTTTAATTTCCAGAAAATCATGTCGGGTGCTGGCAACAGTTGTTAAGGAAAATGAGGAAAAAGTGGTTGTGGAGGATTCATTCCCTCCTAAGGCTTCTCCTGTCCACGACGGGGAAGGAGGCAGTAACCAAGGACCAGAAAATTCATCCTCCAATGGCTTGGATAAATGGATCATTAAGCTTGAACAATCTGTTAATGTTCTTCTCACGGATTCAGTAATAAGGATTCTTGATGCATTTTACCACGATAGACACTACGCAAGGTTCTTTGTCTTGGAAACTATTGCAAGGGTTCCCTATTTTGCGTTTCTGTCAGTTCTGCATTTGTATGAAAGTTTTGGCTGGTGGAGAAGAGCTGATTATCTTAAAGTTCACTTTGCTGAGAGCTGGAATGAGATGCATCACTTGCTGATCATGGAGGAATTGGGGGGTAATGATGGGTGGTTTGATCGATTTCTTGCTCAACATATCGCTGTTGCTTATTATTTTATGACAGTACTGATGTACATGATTAGCCCAAGAATGGCATATCATCTTTCTGAATGTGTTGAAAGCCATGCATTTTCAACTTATGATAAGTTTATAAAGGCCCAAGGAGAGGAGTTGAAAAAATTGCCTGCTCCCGAGGTTGCTGTGAAGTATTACACTGAGGGAGACTTGTACTTATTTGAGAGCTTGTACGATGTGTTTGTGAACATCAGAGACGACGAAGCCGAGCATTGCAAGACCATGAGAGCTTGTCAATCGCATGGGAACCTCCGGTCACCACATTCTTATTCAGAGAGCTATCCTGAAGATGATGAGCCACTCTGCAACCTTGCTGAAGCAGATTGTGAAGGCATTGTAGACTGTATAAAGAAATCCGTTACTTCTTCCCCTCCGGTAAAGCAAAAACTATGA

Protein sequence

MATASSSSSSAVFAISSLQLKPNNVASYRIPNSFSRPSFFARPLISRKSCRVLATVVKENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIRILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHHLLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFIKAQGEELKKLPAPEVAVKYYTEGDLYLFESLYDVFVNIRDDEAEHCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAEADCEGIVDCIKKSVTSSPPVKQKL
Homology
BLAST of Lsi02G010830 vs. ExPASy Swiss-Prot
Match: Q56X52 (Ubiquinol oxidase 4, chloroplastic/chromoplastic OS=Arabidopsis thaliana OX=3702 GN=AOX4 PE=1 SV=2)

HSP 1 Score: 409.1 bits (1050), Expect = 5.0e-113
Identity = 208/310 (67.10%), Postives = 241/310 (77.74%), Query Frame = 0

Query: 41  ARPLISRKSCRVLATVVKENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWI 100
           +R L+ R + RV AT+++++EEKVVVE+SF  KA      E        +SS++  + WI
Sbjct: 44  SRRLLLRNNHRVQATILQDDEEKVVVEESF--KAETSTGTEPLEEPNMSSSSTSAFETWI 103

Query: 101 IKLEQSVNVLLTDSVIRILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRR 160
           IKLEQ VNV LTDSVI+ILD  Y DR YARFFVLETIARVPYFAF+SVLH+YE+FGWWRR
Sbjct: 104 IKLEQGVNVFLTDSVIKILDTLYRDRTYARFFVLETIARVPYFAFMSVLHMYETFGWWRR 163

Query: 161 ADYLKVHFAESWNEMHHLLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYH 220
           ADYLKVHFAESWNEMHHLLIMEELGGN  WFDRFLAQHIA  YYFMTV +Y++SPRMAYH
Sbjct: 164 ADYLKVHFAESWNEMHHLLIMEELGGNSWWFDRFLAQHIATFYYFMTVFLYILSPRMAYH 223

Query: 221 LSECVESHAFSTYDKFIKAQGEELKKLPAPEVAVKYYTEGDLYLF--------------- 280
            SECVESHA+ TYDKF+KA GEELK +PAP++AVKYYT GDLYLF               
Sbjct: 224 FSECVESHAYETYDKFLKASGEELKNMPAPDIAVKYYTGGDLYLFDEFQTSRTPNTRRPV 283

Query: 281 -ESLYDVFVNIRDDEAEHCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNL-AEADCEGI 334
            E+LYDVFVNIRDDEAEHCKTMRACQ+ G+LRSPHS  E    ++E  C +  EA CEGI
Sbjct: 284 IENLYDVFVNIRDDEAEHCKTMRACQTLGSLRSPHSILEDDDTEEESGCVVPEEAHCEGI 343

BLAST of Lsi02G010830 vs. ExPASy Swiss-Prot
Match: O22049 (Ubiquinol oxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX2 PE=1 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 1.9e-11
Identity = 50/164 (30.49%), Postives = 82/164 (50.00%), Query Frame = 0

Query: 120 DAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHHLL 179
           D F+  R+  R  +LET+A VP      +LHL     +     ++K    E+ NE  HL+
Sbjct: 167 DIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLKSIRKFEHSGGWIKALLEEAENERMHLM 226

Query: 180 IMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFIK- 239
            M EL     W++R L   +   ++    + Y+ISPR+A+ +   +E  A  +Y +F+K 
Sbjct: 227 TMMEL-VKPKWYERLLVMLVQGIFFNSFFVCYVISPRLAHRVVGYLEEEAIHSYTEFLKD 286

Query: 240 AQGEELKKLPAPEVAVKYYTEGDLYLFESLYDVFVNIRDDEAEH 283
               +++ + AP +A+ Y+    L    +L DV   IR DEA H
Sbjct: 287 IDNGKIENVAAPAIAIDYWR---LPKDATLKDVVTVIRADEAHH 326

BLAST of Lsi02G010830 vs. ExPASy Swiss-Prot
Match: Q40578 (Ubiquinol oxidase 2, mitochondrial OS=Nicotiana tabacum OX=4097 GN=AOX2 PE=1 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 1.9e-11
Identity = 49/167 (29.34%), Postives = 86/167 (51.50%), Query Frame = 0

Query: 120 DAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHHLL 179
           D F+  R+  R  +LET+A VP      +LH      + +   ++K    E+ NE  HL+
Sbjct: 111 DIFFQRRYGCRAMMLETVAAVPGMVGGMLLHCKSLRRFEQSGGWIKALLEEAENERMHLM 170

Query: 180 IMEELGGNDGWFDR---FLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKF 239
              E+     W++R   F  Q + +  YF+T   Y++SP++A+ +   +E  A  +Y +F
Sbjct: 171 TFMEV-AKPNWYERALVFAVQGVFINAYFVT---YLLSPKLAHRIVGYLEEEAIHSYTEF 230

Query: 240 IKAQGE-ELKKLPAPEVAVKYYTEGDLYLFESLYDVFVNIRDDEAEH 283
           +K   +  ++ +PAP +A+ Y+    L    +L DV + +R DEA H
Sbjct: 231 LKELDKGNIENVPAPAIAIDYWR---LPKDSTLRDVVLVVRADEAHH 270

BLAST of Lsi02G010830 vs. ExPASy Swiss-Prot
Match: Q8W855 (Ubiquinol oxidase 1c, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=AOX1C PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-11
Identity = 51/169 (30.18%), Postives = 86/169 (50.89%), Query Frame = 0

Query: 120 DAFYHDRHYARFFVLETIARVPYFAFLSVLHL-----YESFGWWRRADYLKVHFAESWNE 179
           D F+  R+  R  +LET+A VP      +LHL     +E  G W RA        E+ NE
Sbjct: 159 DIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLRSLRRFEHSGGWIRA-----LLEEAENE 218

Query: 180 MHHLLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYD 239
             HL+   E+     W++R L   +   ++    L Y++SP++A+ +   +E  A  +Y 
Sbjct: 219 RMHLMTFMEV-AKPRWYERALVLAVQGVFFNAYFLGYLLSPKLAHRVVGYLEEEAIHSYT 278

Query: 240 KFIK-AQGEELKKLPAPEVAVKYYTEGDLYLFESLYDVFVNIRDDEAEH 283
           +++K  +  +++ +PAP +A+ Y+    L    +L DV V +R DEA H
Sbjct: 279 EYLKDIEAGKIENVPAPPIAIDYWR---LPAGATLKDVVVVVRADEAHH 318

BLAST of Lsi02G010830 vs. ExPASy Swiss-Prot
Match: O22048 (Ubiquinol oxidase 1c, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX1C PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.2e-11
Identity = 46/164 (28.05%), Postives = 82/164 (50.00%), Query Frame = 0

Query: 120 DAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHHLL 179
           D F+  R+  R  +LET+A VP      ++H      + +   ++K    E+ NE  HL+
Sbjct: 143 DLFFQRRYGCRAIMLETVAAVPGMVGGMLMHFKSLRRFEQSGGWIKALLEEAENERMHLM 202

Query: 180 IMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFIK- 239
              E+     W++R L   +   ++   ++ Y+ISP+ A+ +   +E  A  +Y +F+K 
Sbjct: 203 TFMEV-AKPKWYERALVISVQGVFFNAYLIGYIISPKFAHRMVGYLEEEAIHSYTEFLKE 262

Query: 240 AQGEELKKLPAPEVAVKYYTEGDLYLFESLYDVFVNIRDDEAEH 283
                ++ +PAP +AV Y+    L    +L DV + +R DEA H
Sbjct: 263 LDNGNIENVPAPAIAVDYWR---LEADATLRDVVMVVRADEAHH 302

BLAST of Lsi02G010830 vs. ExPASy TrEMBL
Match: A0A5A7UI87 (Ubiquinol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00950 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.1e-169
Identity = 306/360 (85.00%), Postives = 325/360 (90.28%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKPN---NVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+ A SSSSSAVFAISS++LKP+   NV S+RIP SFS     ARPLISRKSCRV+ATV+
Sbjct: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EEKVV+E+SFPPKASPV DGEGGS QGP++SSSNGLDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLF                ESLYDVFVNIRDDEAE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAEADCEGIVDCIKKSVTSSPPVKQKL 342
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNLAEADCEGIVDCIKKS+TSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Lsi02G010830 vs. ExPASy TrEMBL
Match: A0A1S3C992 (Ubiquinol oxidase OS=Cucumis melo OX=3656 GN=LOC103498237 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.1e-169
Identity = 306/360 (85.00%), Postives = 325/360 (90.28%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKPN---NVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+ A SSSSSAVFAISS++LKP+   NV S+RIP SFS     ARPLISRKSCRV+ATV+
Sbjct: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EEKVV+E+SFPPKASPV DGEGGS QGP++SSSNGLDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLF                ESLYDVFVNIRDDEAE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAEADCEGIVDCIKKSVTSSPPVKQKL 342
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNLAEADCEGIVDCIKKS+TSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Lsi02G010830 vs. ExPASy TrEMBL
Match: A0A0A0M130 (Ubiquinol oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G699610 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.4e-166
Identity = 300/360 (83.33%), Postives = 322/360 (89.44%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKP---NNVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+ A SSSSS +FA SS++LKP    N+AS+RIP SFS     ARPLISRKSCRV+ATV+
Sbjct: 1   MSIALSSSSSPLFAFSSVELKPINTKNLASFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EEKVV+E+SFPPKASP+ DGEGGS QGPE+SSSNG DKWI+KLEQSVNVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPIQDGEGGSGQGPESSSSNGFDKWIVKLEQSVNVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLF                ESLYDVF+NIRDDEAE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFMNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAEADCEGIVDCIKKSVTSSPPVKQKL 342
           HCKTMRACQSHGNLRSPHSYSES PE+DEPLCNLAEADCEGIVDCIKKS+TSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEEDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Lsi02G010830 vs. ExPASy TrEMBL
Match: A0A6J1GSZ7 (Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457254 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.3e-164
Identity = 301/361 (83.38%), Postives = 320/361 (88.64%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKPN---NVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+ A  SSSSA+F  SS+QLKP    N+ S+RI  +FSR SF +RPL+SRKSCRVLATV+
Sbjct: 1   MSIAWCSSSSALFGTSSVQLKPYNTINIDSFRIGTAFSRRSFSSRPLVSRKSCRVLATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EE+VV+E+SFPPKASPV  GEGGS  G ENSSSNGLDKWIIKLEQSVNVLLTDSVIR
Sbjct: 61  KEDEEEVVIEESFPPKASPVEKGEGGSEGGAENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAFLSVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV+MYMISPRMAYHLSECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLF                E+LYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIENLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAE-ADCEGIVDCIKKSVTSSPPVKQK 342
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNL E ADCEGIVDC+KK+VTSSPPVK K
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESRPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKPK 360

BLAST of Lsi02G010830 vs. ExPASy TrEMBL
Match: A0A6J1K2B2 (Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111490026 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 6.5e-164
Identity = 299/361 (82.83%), Postives = 320/361 (88.64%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKP---NNVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+ A +SSSSA+F  SS+QL P    N+AS+RI  +FSR SF +RPL+SRK CRVLATV+
Sbjct: 1   MSIAWTSSSSALFGTSSVQLNPYNTKNIASFRIGTAFSRRSFSSRPLVSRKLCRVLATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EE+VV+E+SFPPKASPV  GEGGS  G ENSSSN LDKWIIKLEQSVNVLLTDSVIR
Sbjct: 61  KEDEEEVVIEESFPPKASPVDRGEGGSEGGAENSSSNALDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAFLSVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV+MYMISPRMAYHLSECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLF                ++LYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIDNLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAE-ADCEGIVDCIKKSVTSSPPVKQK 342
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNL E ADCEGIVDC+KK+VTSSPPVKQK
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKQK 360

BLAST of Lsi02G010830 vs. NCBI nr
Match: XP_038893742.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Benincasa hispida])

HSP 1 Score: 613.2 bits (1580), Expect = 1.3e-171
Identity = 313/360 (86.94%), Postives = 323/360 (89.72%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKPN---NVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+TA SS SSAVFAISS+QLKPN   NVAS+RIP SF R SF A PLISRKSCRVLATV+
Sbjct: 1   MSTAWSSLSSAVFAISSVQLKPNNTKNVASFRIPTSFPRRSFVAHPLISRKSCRVLATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EEKVVVEDSFPPKASPV DGEGGSN+GPE S SNG DKWIIKLEQSVNV LTDSVIR
Sbjct: 61  KEDEEKVVVEDSFPPKASPVQDGEGGSNEGPEISPSNGFDKWIIKLEQSVNVFLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAFLSVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLF                ESLYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAEADCEGIVDCIKKSVTSSPPVKQKL 342
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNL+EADCEGIVDCIKKS+T S PVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLSEADCEGIVDCIKKSITFSHPVKQKL 360

BLAST of Lsi02G010830 vs. NCBI nr
Match: XP_008458986.1 (PREDICTED: ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Cucumis melo] >KAA0054287.1 ubiquinol oxidase 4 [Cucumis melo var. makuwa] >TYK12325.1 ubiquinol oxidase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 603.6 bits (1555), Expect = 1.1e-168
Identity = 306/360 (85.00%), Postives = 325/360 (90.28%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKPN---NVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+ A SSSSSAVFAISS++LKP+   NV S+RIP SFS     ARPLISRKSCRV+ATV+
Sbjct: 1   MSIALSSSSSAVFAISSVELKPSNTKNVTSFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EEKVV+E+SFPPKASPV DGEGGS QGP++SSSNGLDKWIIKLEQS+NVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPVQDGEGGSGQGPQSSSSNGLDKWIIKLEQSINVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLF                ESLYDVFVNIRDDEAE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAEADCEGIVDCIKKSVTSSPPVKQKL 342
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNLAEADCEGIVDCIKKS+TSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Lsi02G010830 vs. NCBI nr
Match: XP_004145426.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucumis sativus])

HSP 1 Score: 595.5 bits (1534), Expect = 2.9e-166
Identity = 300/360 (83.33%), Postives = 322/360 (89.44%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKP---NNVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+ A SSSSS +FA SS++LKP    N+AS+RIP SFS     ARPLISRKSCRV+ATV+
Sbjct: 1   MSIALSSSSSPLFAFSSVELKPINTKNLASFRIPTSFS-----ARPLISRKSCRVMATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EEKVV+E+SFPPKASP+ DGEGGS QGPE+SSSNG DKWI+KLEQSVNVLLTDSVI+
Sbjct: 61  KEDEEKVVLEESFPPKASPIQDGEGGSGQGPESSSSNGFDKWIVKLEQSVNVLLTDSVIK 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHYARFFVLETIARVPYFAF+SVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYARFFVLETIARVPYFAFVSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV MYMISPRMAYH SECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVFMYMISPRMAYHFSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KA+GEELKK PAPEVAVKYYTEGDLYLF                ESLYDVF+NIRDDEAE
Sbjct: 241 KAEGEELKKQPAPEVAVKYYTEGDLYLFDEFQTSRAPKSRRPKIESLYDVFMNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAEADCEGIVDCIKKSVTSSPPVKQKL 342
           HCKTMRACQSHGNLRSPHSYSES PE+DEPLCNLAEADCEGIVDCIKKS+TSSPPVKQKL
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEEDEPLCNLAEADCEGIVDCIKKSITSSPPVKQKL 355

BLAST of Lsi02G010830 vs. NCBI nr
Match: XP_022955227.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita moschata])

HSP 1 Score: 589.0 bits (1517), Expect = 2.7e-164
Identity = 301/361 (83.38%), Postives = 320/361 (88.64%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKPN---NVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+ A  SSSSA+F  SS+QLKP    N+ S+RI  +FSR SF +RPL+SRKSCRVLATV+
Sbjct: 1   MSIAWCSSSSALFGTSSVQLKPYNTINIDSFRIGTAFSRRSFSSRPLVSRKSCRVLATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EE+VV+E+SFPPKASPV  GEGGS  G ENSSSNGLDKWIIKLEQSVNVLLTDSVIR
Sbjct: 61  KEDEEEVVIEESFPPKASPVEKGEGGSEGGAENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAFLSVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV+MYMISPRMAYHLSECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLF                E+LYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIENLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAE-ADCEGIVDCIKKSVTSSPPVKQK 342
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNL E ADCEGIVDC+KK+VTSSPPVK K
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESRPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKPK 360

BLAST of Lsi02G010830 vs. NCBI nr
Match: XP_022994224.1 (ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita maxima])

HSP 1 Score: 586.6 bits (1511), Expect = 1.3e-163
Identity = 299/361 (82.83%), Postives = 320/361 (88.64%), Query Frame = 0

Query: 1   MATASSSSSSAVFAISSLQLKP---NNVASYRIPNSFSRPSFFARPLISRKSCRVLATVV 60
           M+ A +SSSSA+F  SS+QL P    N+AS+RI  +FSR SF +RPL+SRK CRVLATV+
Sbjct: 1   MSIAWTSSSSALFGTSSVQLNPYNTKNIASFRIGTAFSRRSFSSRPLVSRKLCRVLATVL 60

Query: 61  KENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWIIKLEQSVNVLLTDSVIR 120
           KE+EE+VV+E+SFPPKASPV  GEGGS  G ENSSSN LDKWIIKLEQSVNVLLTDSVIR
Sbjct: 61  KEDEEEVVIEESFPPKASPVDRGEGGSEGGAENSSSNALDKWIIKLEQSVNVLLTDSVIR 120

Query: 121 ILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHH 180
           ILDAFYHDRHY RFFVLETIARVPYFAFLSVLH+YESFGWWRRADYLKVHFAESWNEMHH
Sbjct: 121 ILDAFYHDRHYPRFFVLETIARVPYFAFLSVLHMYESFGWWRRADYLKVHFAESWNEMHH 180

Query: 181 LLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFI 240
           LLIMEELGGND WFDRFLAQHIAVAYYFMTV+MYMISPRMAYHLSECVESHAFSTYDKF+
Sbjct: 181 LLIMEELGGNDWWFDRFLAQHIAVAYYFMTVVMYMISPRMAYHLSECVESHAFSTYDKFL 240

Query: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLF----------------ESLYDVFVNIRDDEAE 300
           KAQGEELKKLPAPEVAVKYYTEGDLYLF                ++LYDVFVNIRDDEAE
Sbjct: 241 KAQGEELKKLPAPEVAVKYYTEGDLYLFDEFQTSRAPNSRRPKIDNLYDVFVNIRDDEAE 300

Query: 301 HCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNLAE-ADCEGIVDCIKKSVTSSPPVKQK 342
           HCKTMRACQSHGNLRSPHSYSES PEDDEPLCNL E ADCEGIVDC+KK+VTSSPPVKQK
Sbjct: 301 HCKTMRACQSHGNLRSPHSYSESCPEDDEPLCNLPEAADCEGIVDCLKKAVTSSPPVKQK 360

BLAST of Lsi02G010830 vs. TAIR 10
Match: AT4G22260.1 (Alternative oxidase family protein )

HSP 1 Score: 409.1 bits (1050), Expect = 3.6e-114
Identity = 208/310 (67.10%), Postives = 241/310 (77.74%), Query Frame = 0

Query: 41  ARPLISRKSCRVLATVVKENEEKVVVEDSFPPKASPVHDGEGGSNQGPENSSSNGLDKWI 100
           +R L+ R + RV AT+++++EEKVVVE+SF  KA      E        +SS++  + WI
Sbjct: 44  SRRLLLRNNHRVQATILQDDEEKVVVEESF--KAETSTGTEPLEEPNMSSSSTSAFETWI 103

Query: 101 IKLEQSVNVLLTDSVIRILDAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRR 160
           IKLEQ VNV LTDSVI+ILD  Y DR YARFFVLETIARVPYFAF+SVLH+YE+FGWWRR
Sbjct: 104 IKLEQGVNVFLTDSVIKILDTLYRDRTYARFFVLETIARVPYFAFMSVLHMYETFGWWRR 163

Query: 161 ADYLKVHFAESWNEMHHLLIMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYH 220
           ADYLKVHFAESWNEMHHLLIMEELGGN  WFDRFLAQHIA  YYFMTV +Y++SPRMAYH
Sbjct: 164 ADYLKVHFAESWNEMHHLLIMEELGGNSWWFDRFLAQHIATFYYFMTVFLYILSPRMAYH 223

Query: 221 LSECVESHAFSTYDKFIKAQGEELKKLPAPEVAVKYYTEGDLYLF--------------- 280
            SECVESHA+ TYDKF+KA GEELK +PAP++AVKYYT GDLYLF               
Sbjct: 224 FSECVESHAYETYDKFLKASGEELKNMPAPDIAVKYYTGGDLYLFDEFQTSRTPNTRRPV 283

Query: 281 -ESLYDVFVNIRDDEAEHCKTMRACQSHGNLRSPHSYSESYPEDDEPLCNL-AEADCEGI 334
            E+LYDVFVNIRDDEAEHCKTMRACQ+ G+LRSPHS  E    ++E  C +  EA CEGI
Sbjct: 284 IENLYDVFVNIRDDEAEHCKTMRACQTLGSLRSPHSILEDDDTEEESGCVVPEEAHCEGI 343

BLAST of Lsi02G010830 vs. TAIR 10
Match: AT5G64210.1 (alternative oxidase 2 )

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-12
Identity = 50/164 (30.49%), Postives = 82/164 (50.00%), Query Frame = 0

Query: 120 DAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHHLL 179
           D F+  R+  R  +LET+A VP      +LHL     +     ++K    E+ NE  HL+
Sbjct: 167 DIFFQRRYGCRAMMLETVAAVPGMVGGMLLHLKSIRKFEHSGGWIKALLEEAENERMHLM 226

Query: 180 IMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFIK- 239
            M EL     W++R L   +   ++    + Y+ISPR+A+ +   +E  A  +Y +F+K 
Sbjct: 227 TMMEL-VKPKWYERLLVMLVQGIFFNSFFVCYVISPRLAHRVVGYLEEEAIHSYTEFLKD 286

Query: 240 AQGEELKKLPAPEVAVKYYTEGDLYLFESLYDVFVNIRDDEAEH 283
               +++ + AP +A+ Y+    L    +L DV   IR DEA H
Sbjct: 287 IDNGKIENVAAPAIAIDYWR---LPKDATLKDVVTVIRADEAHH 326

BLAST of Lsi02G010830 vs. TAIR 10
Match: AT3G27620.1 (alternative oxidase 1C )

HSP 1 Score: 69.7 bits (169), Expect = 5.1e-12
Identity = 46/164 (28.05%), Postives = 82/164 (50.00%), Query Frame = 0

Query: 120 DAFYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHHLL 179
           D F+  R+  R  +LET+A VP      ++H      + +   ++K    E+ NE  HL+
Sbjct: 143 DLFFQRRYGCRAIMLETVAAVPGMVGGMLMHFKSLRRFEQSGGWIKALLEEAENERMHLM 202

Query: 180 IMEELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFIK- 239
              E+     W++R L   +   ++   ++ Y+ISP+ A+ +   +E  A  +Y +F+K 
Sbjct: 203 TFMEV-AKPKWYERALVISVQGVFFNAYLIGYIISPKFAHRMVGYLEEEAIHSYTEFLKE 262

Query: 240 AQGEELKKLPAPEVAVKYYTEGDLYLFESLYDVFVNIRDDEAEH 283
                ++ +PAP +AV Y+    L    +L DV + +R DEA H
Sbjct: 263 LDNGNIENVPAPAIAVDYWR---LEADATLRDVVMVVRADEAHH 302

BLAST of Lsi02G010830 vs. TAIR 10
Match: AT1G32350.1 (alternative oxidase 1D )

HSP 1 Score: 68.9 bits (167), Expect = 8.7e-12
Identity = 47/162 (29.01%), Postives = 80/162 (49.38%), Query Frame = 0

Query: 122 FYHDRHYARFFVLETIARVPYFAFLSVLHLYESFGWWRRADYLKVHFAESWNEMHHLLIM 181
           F+  +H     +LET+A VP      +LHL     +     ++K    E+ NE  HL+  
Sbjct: 134 FFQRKHMCHAMLLETVAAVPGMVGGMLLHLKSLRRFEHSGGWIKALLEEAENERMHLMTF 193

Query: 182 EELGGNDGWFDRFLAQHIAVAYYFMTVLMYMISPRMAYHLSECVESHAFSTYDKFIK-AQ 241
            EL     W++R +   +   ++    L Y+ISP++A+ ++  +E  A ++Y +F+K   
Sbjct: 194 IEL-SQPKWYERAIVFTVQGVFFNAYFLAYVISPKLAHRITGYLEEEAVNSYTEFLKDID 253

Query: 242 GEELKKLPAPEVAVKYYTEGDLYLFESLYDVFVNIRDDEAEH 283
             + +  PAP +A+ Y+    L    +L DV   IR DEA H
Sbjct: 254 AGKFENSPAPAIAIDYWR---LPKDATLRDVVYVIRADEAHH 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q56X525.0e-11367.10Ubiquinol oxidase 4, chloroplastic/chromoplastic OS=Arabidopsis thaliana OX=3702... [more]
O220491.9e-1130.49Ubiquinol oxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX2 PE=1 ... [more]
Q405781.9e-1129.34Ubiquinol oxidase 2, mitochondrial OS=Nicotiana tabacum OX=4097 GN=AOX2 PE=1 SV=... [more]
Q8W8552.5e-1130.18Ubiquinol oxidase 1c, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
O220487.2e-1128.05Ubiquinol oxidase 1c, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX1C PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7UI875.1e-16985.00Ubiquinol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00... [more]
A0A1S3C9925.1e-16985.00Ubiquinol oxidase OS=Cucumis melo OX=3656 GN=LOC103498237 PE=3 SV=1[more]
A0A0A0M1301.4e-16683.33Ubiquinol oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G699610 PE=3 SV=1[more]
A0A6J1GSZ71.3e-16483.38Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457254 PE=3 SV=1[more]
A0A6J1K2B26.5e-16482.83Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111490026 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038893742.11.3e-17186.94ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Benincasa hispida][more]
XP_008458986.11.1e-16885.00PREDICTED: ubiquinol oxidase 4, chloroplastic/chromoplastic-like [Cucumis melo] ... [more]
XP_004145426.12.9e-16683.33ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucumis sativus][more]
XP_022955227.12.7e-16483.38ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita moschata][more]
XP_022994224.11.3e-16382.83ubiquinol oxidase 4, chloroplastic/chromoplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G22260.13.6e-11467.10Alternative oxidase family protein [more]
AT5G64210.11.3e-1230.49alternative oxidase 2 [more]
AT3G27620.15.1e-1228.05alternative oxidase 1C [more]
AT1G32350.18.7e-1229.01alternative oxidase 1D [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002680Alternative oxidasePFAMPF01786AOXcoord: 111..289
e-value: 4.7E-38
score: 131.0
IPR002680Alternative oxidasePANTHERPTHR31803ALTERNATIVE OXIDASEcoord: 34..327
IPR002680Alternative oxidaseCDDcd01053AOXcoord: 123..293
e-value: 3.61076E-97
score: 283.329
IPR038659Alternative oxidase superfamilyGENE3D1.20.1260.140Alternative oxidasecoord: 54..299
e-value: 2.5E-62
score: 212.3
NoneNo IPR availablePIRSRPIRSR005229-1PIRSR005229-1coord: 125..285
e-value: 2.2E-19
score: 66.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..92
NoneNo IPR availablePANTHERPTHR31803:SF10UBIQUINOL OXIDASE 4, CHLOROPLASTIC/CHROMOPLASTICcoord: 34..327

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G010830.1Lsi02G010830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010230 alternative respiration
biological_process GO:0016117 carotenoid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0070469 respirasome
cellular_component GO:0009579 thylakoid
molecular_function GO:0009916 alternative oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0102721 ubiquinol:oxygen oxidoreductase activity