Lsi02G010530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G010530
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProteasome subunit alpha type
Locationchr02: 11395511 .. 11400844 (-)
RNA-Seq ExpressionLsi02G010530
SyntenyLsi02G010530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACCGGGCGAAAATGGCTAAACCAAAAACCCGATGGACTCCAGAAATCAACCCGAAGAAAACCCCCGCATCATCATCTTGCTTCGCAAGAAAGGGTCTGCTTAAAGCTTACTGAACAAAGCTGTTTTGCTGCTTCTTCTTCTTCTTCTTCCTTCAGTGTTTCAGGCAAGTAGATCTCTGTCTTCTCCACACACTCTCTTGGTTTTCTGTATTCGAATTTTCATCCTATTGGAATTTTGGCGCTCTTGATATGGTTCTGATTTCTCAGTGTTTGTGCAGAATCTAGCCGGTTTCTTCAATTGAATCGCAAAATGTTTCTCACCAGGTGATCCAGTGCATTCTCTTCTCCTCTACAGCATTTTCGTTCTCTCTTTGTTTTTGTGTTGTTTCCATTGTTTAATTTTGCGGTCTCTCTTTTTGTGCTTCTGGTTCTGGGTTTTTTCAGGACCGAGTATGATAGGGGTGTGAATACCTTCTCTCCGGAAGGTCGATTGTTCCAGGTTGAGTACGCGATTGAGGCTATTAAGGTAGTTTATTGTTTTTCTTCCCTTTCGCACTCTCATTTCTGGGTTATTTTCATTAAAAATCGTGGAAAAGGTTGTGAGATTCGGAGAAGGGTAGGGAAGATGAGTGACAGAATCTTACATGCCTCTTTTTTTGTCCATTTTATTGAATCTGCTGATTTCCCTCAATTTTTTGCAGCTTGGTTCGACGGCAATTGGTTTGAAAACAAAGGAAGGCGTTGTGCTTGCGGTCGAGAAACGTATTACATCTCCACTACTGGTCTGCCCCTTTTCCTTAGAATTTTCTAAATTTCTTTGTTATTTGTTGTCAACTGCATATATTATCATATTCAGCTTAAATTAGATGCCTAGCAGTCTACGTAGACGGTAGACATTATAATAGATATGGGACATATAAGGTTATATGCAAGCTGAACTTAAATATCTTGCCGTCAATGTTGAAACTGAAAGTTGAACTTTGGTTTAACTGAAATTTAGTTATTGACCCATCTTCTTATCTGCTTGTATTTCAAAGCTCCATTTCTTATGACGAAAAGGCTCAAACATTGCTTAGAGAGGGGTCTTTATCAGTGTGACACCAGATTGTTTTGGGAGTTCATTTCTGTTTTTTAATGGAACTATTTCCTCTCATGTGGGCTTCATGAAGTTTTGGTTATATTCGTTCTGATACACATCTTCCTCTAGTCCTTTGTGCTACTTTTGTCTTTGGTCCAATCTGCAGCTTATTGTATCACATCCTTTATAGATTGGCTTGTATCTTTATCTATTCCTACTTTGTACATCCATATTTGTATCTTTCGGATTGGGTTCGGCCTTTGTATTGTTTATGGGTGATATCTCTATTGGCTAATCCCTTTTGTACTCTTTGGATGTTTCATTCATCAATGAAACTGTTTCTTATCCAACAAAAAAAAAATTGTGATATTTTAGCTCAGACACTCTTTTATATTTTATTTATACATTTTTGGAACTTTTGTATAGGAACCAAGTAGCGTGGAAAAAATCATGGAAATTGATGAGCATATAGGATGTGCAATGAGTGGACTAATTGCTGATGCTCACACACTTGTTGAACATGCTCGAGTTGAGACCCAGGTGCTGTTGATGCTCTTGCCTTGCATCTATAGTTGATATCTTTTAAAGACAAACTATTGAATTTTGAACTTCATGTTCTGCAGAACCATCGGTTCTCATATGGCGAACCAATGACTGTAGAGTCTACAACACAAGCTCTTTGTGATCTAGCCCTTAGATTTGGCGAAGGCGATGAGGAGTCCATGGTTATTACCACAACCTTTTTTCTATTCATTTTACTCTCTCTTCTCACTTCCTGTTAAATTAATCTAAATTGGCTTTAAAATGGCAATGACTTAACACCATTTATTTCGTTACTTAGCTTCCATTCAAAGTATAACCTTCACATGTATTATTTGGTTAGGGAATTATTTCATGCTTAATATTTACAGGTGTAAAAAATGAGTTTTTGGATAAGTGACTTTCTGATGCTATTGACATGAATGCATGCAGAAATTAGTATACTTTTCACATGATTTTGTTTGAATTGAGAATGGTATCCTGTACTAGTAAAAAGTAGGAGGGCTGTATTCCCCATTTCCATCTTTTGTATTTGAAAGAATAAGATCTCTTGTATTCTTCTTATGATGAGAATGGATACTTGGTGTTATTATTTCACTAATACAATACATTTTTTTTTCTCTGTACAGTCTCGACCATTTGGTGTAGCTCTTCTCATTGCCGGCCATGATGAGAAGGGGCCCAGCTTGTAAGTTTTACACTGTTAAAGAATAAACTGATTATGTGATTAGCTGCTATTTCACTGCATTATGTATTTTTCACCCCCACCTCCACAGCCCAAGTGTTGGCTGTATGGATGTGTGCACGCTTTTTTTGCATCCTTCTTTTTGCATGGCATGGAAAAAGAGGTTGCATCATTTTATCTAAGAATAAAAACATCCCTTGTTGGGTACTTTTCTCGTTGAGGTACTTCATACTTCTACTAATCACACGCTCAGGGAAAAAGTTCAAATTGCAACCGTTGAAATTTATATGCGATATTGTGTGATGAGCTGCATTCAAATACCTTTTTGATGTGAGACATGTAGAGTGTAGACAAACAATATAAATTGGAGAATTAATGGTCATCCCCAACCCTAATGACAGAGGAGATTACAATTTGAAAAGGTTCCAATCAGCCAAAAGTCAAGCAAATGATAACATCAGACTGCTATTCTTTGATAATTTAATGTACCCTCAAAGAGATCGTAAATTCACATGGGTCCTCAAATAATACGCACTTTATCATTTTTATCTTAGATCCTTATATTTTTCCTTCAGTAGTTCAATCACCCTCAAATAACCAAGATAGATACAAAGCTAGTGTTTCTAGGTTTTCCTTTGAGATAAGAACCAAATGACAGTTAGAGGAGTAACCCAAGCATCTTATAAAGATTGTGACGTCTCTCTATCTTTCCAATGTGGGATCTTCAACACGCCTTCTCAAGATGGTGCCTCTTTGGGTTCATCATTCTTGGGTTGGTTTTATTTTTATTTTTTTTTTGGAATCGAATACTTATTTGGGCTCCATGAGTTCTTATGCCATATTAGATTAACGTGAAATTTCATAGTTTTGACAACGAGAGGAGTAACTCATGTATCTCTTTGGGATAAATAATTTAAGGCAGAGTGATGATAAGGCTGTAAAATTAGGGATGTGTCAGTTTATAGTAGATTTAGGTGAATTTGTTAGGGATGGTTACAACTAGAATACAAAGACATCCCGGTATTTCTTGTATCAACGTAGAAATATAATTAAACATTTTCTGTGATAATATGCACGTCAGTGATTCTCCTAGGGTGTTGCTTCTGAATTTACCTTTTTCATGATTTTTCTCTTTGCTTGATTATTGTTGTTTTACTGATGTTTATGCTTTCTATTCTACTTTTTACTGGGTTCTGTCTCATGCATGTGCCTTTGAGCCTTATGGAACGAGTTTTTCGTTTGGCACTCATATTTTTAATTTTAAGTACTCTCAACAATTAGAACATTAAGTCTTCTGCGGAGGCCACTACATTGTTTTGCTTTTTAATGTCAAATAATCTTTATATTTATTATTGATTATTACTTGTTTGCATCACCTATGCAATTTTTGATGAAGTGTTCATTTTTACTTGTGTAGATTACTGCTAGCAGTGTATACTGTTTTAATATCAAATTTTTTGATGAAGTGTTCATATCAGAATTTTGTGCATGATTAGGAATCTGCATTATGGATTAGCTAAAATTTCCTCTGCTCATATGCTGGCAGAAATGACCCCTCCCCACCGCCCAGTTTTTAACCTGATAATCTGTGGTATGCAGATTCTACACTGATCCATCTGGCACTTTCTGGCAATGCAATGCTAAAGCTATCGGTTCAGGTTCTGAAGGTGCAGATAGCTCTCTGCAAGAGCAATACAACAAGGTATGCTTTGTACTTTATTGGTGTCAGATATAACTGAATGTTATTGTCGCGTATCCATTTCCTCTTCCATTTTCAGAAAAAAAACTAGACAGATAAAGCACATCCTGACCTCATAGTTGTCTAATCATAGGTCTTTGGTAGCTAAACTTGTTGAAGGACAGAAATTTATTAAGATATGTGGTCACTAAGAGAACATTTTATTTACAAGCAATATGAAGTCTGGTGCTTTCGAAGTTGAAATGTGAAAAGTCGATATATGTATATATGAGATATGTCTGATCAAGAGGTTAAGTGTAGAACAGCTATTTCTCTATATTCTTTATGATTATAATTATTTGAGATAAATCTTACCATTTTCCTCTGACTATATCGTTTCAGACAGTCCTACAAGCATTGTAGTAAACGTCTGCGATTGTTACACCCATGCATGCTTAAGCAACTACATATCTCAAAGTTAACACCATATGTTTTTGCCACATATATTAAAAGGAGTTCTTGCTTTTTGTTCAATTTCCACATACACCAATATTATCTTCAATTGATTGCAGGACCTAACTCTTCAAGAAGCTGAAACCATAGCACTTTCCATTCTGAAGCAAGTTATGGAAGAGAAGGTAAATAAATAGGGCTTTCGATTCTAAACTGCTTGCGTTAAACATTGGAATGAAATATCAAGAAGCATTGCACTTCTAGCATTAACTTTTACATCTAATAACATGTCCATTAAACCGCTCCCCTCCTATTTCAGGTAACTCCCAACAATGTCGACATTGCGAAGGTGTCTCCAACATACCATCTGTACACCCCTGCGGAGGTGGAGGCGGTCATTAGCCGGCTATGAGAGGTTCTTTCGAAAATCTTCTGGACTGCTGTTATGTTTCTTGTCACTGGCATGTATTTGTACTCTTCTTTGTCTGGTGAGGGAGAATCTCAGGTTTTCGGCTACTCTCCAAGCTATTCGTGATGTGTTTATCGATATCTTGCAGATTAATTATGGGACGTTCTTGATGGGTTATTTCGAACTTAGATGAACAATTTGATTAGTCCTTTTACGAAAGCTCTGAACATTATTCTGATGCGCCATTTTGTTTCAAAACGTGAATATCTTGTGACAAACAGGAAAAATTTGAGTTAGCAGCAAGCAGATTCATAGTCTATAATCTTTTTCGTCTTCTTCCCTTTTTGTTTTTGTTTTTGTTTCTGGAATGTGACCCATAGATGCATTTATTTGGTTATCATATTTGATATCTTCTTAGATGTAGGAATGATGAGAACATTATGATAGGGAAAAGAAAGGAATATAGGGGCTTCATCCTTTTAAATGTC

mRNA sequence

ATGAACCGGGCGAAAATGGCTAAACCAAAAACCCGATGGACTCCAGAAATCAACCCGAAGAAAACCCCCGCATCATCATCTTGCTTCGCAAGAAAGGAATCTAGCCGGTTTCTTCAATTGAATCGCAAAATGTTTCTCACCAGGACCGAGTATGATAGGGGTGTGAATACCTTCTCTCCGGAAGGTCGATTGTTCCAGGTTGAGTACGCGATTGAGGCTATTAAGCTTGGTTCGACGGCAATTGGTTTGAAAACAAAGGAAGGCGTTGTGCTTGCGGTCGAGAAACGTATTACATCTCCACTACTGGAACCAAGTAGCGTGGAAAAAATCATGGAAATTGATGAGCATATAGGATGTGCAATGAGTGGACTAATTGCTGATGCTCACACACTTGTTGAACATGCTCGAGTTGAGACCCAGAACCATCGGTTCTCATATGGCGAACCAATGACTGTAGAGTCTACAACACAAGCTCTTTGTGATCTAGCCCTTAGATTTGGCGAAGGCGATGAGGAGTCCATGTCTCGACCATTTGGTGTAGCTCTTCTCATTGCCGGCCATGATGAGAAGGGGCCCAGCTTATTCTACACTGATCCATCTGGCACTTTCTGGCAATGCAATGCTAAAGCTATCGGTTCAGGTTCTGAAGGTGCAGATAGCTCTCTGCAAGAGCAATACAACAAGGACCTAACTCTTCAAGAAGCTGAAACCATAGCACTTTCCATTCTGAAGCAAGTTATGGAAGAGAAGGTAACTCCCAACAATGTCGACATTGCGAAGGTGTCTCCAACATACCATCTGTACACCCCTGCGGAGGTGGAGGCGGTCATTAGCCGGCTATGAGAGGTTCTTTCGAAAATCTTCTGGACTGCTGTTATGTTTCTTGTCACTGGCATGTATTTGTACTCTTCTTTGTCTGGTGAGGGAGAATCTCAGGTTTTCGGCTACTCTCCAAGCTATTCGTGATGTGTTTATCGATATCTTGCAGATTAATTATGGGACGTTCTTGATGGGTTATTTCGAACTTAGATGAACAATTTGATTAGTCCTTTTACGAAAGCTCTGAACATTATTCTGATGCGCCATTTTGTTTCAAAACGTGAATATCTTGTGACAAACAGGAAAAATTTGAGTTAGCAGCAAGCAGATTCATAGTCTATAATCTTTTTCGTCTTCTTCCCTTTTTGTTTTTGTTTTTGTTTCTGGAATGTGACCCATAGATGCATTTATTTGGTTATCATATTTGATATCTTCTTAGATGTAGGAATGATGAGAACATTATGATAGGGAAAAGAAAGGAATATAGGGGCTTCATCCTTTTAAATGTC

Coding sequence (CDS)

ATGAACCGGGCGAAAATGGCTAAACCAAAAACCCGATGGACTCCAGAAATCAACCCGAAGAAAACCCCCGCATCATCATCTTGCTTCGCAAGAAAGGAATCTAGCCGGTTTCTTCAATTGAATCGCAAAATGTTTCTCACCAGGACCGAGTATGATAGGGGTGTGAATACCTTCTCTCCGGAAGGTCGATTGTTCCAGGTTGAGTACGCGATTGAGGCTATTAAGCTTGGTTCGACGGCAATTGGTTTGAAAACAAAGGAAGGCGTTGTGCTTGCGGTCGAGAAACGTATTACATCTCCACTACTGGAACCAAGTAGCGTGGAAAAAATCATGGAAATTGATGAGCATATAGGATGTGCAATGAGTGGACTAATTGCTGATGCTCACACACTTGTTGAACATGCTCGAGTTGAGACCCAGAACCATCGGTTCTCATATGGCGAACCAATGACTGTAGAGTCTACAACACAAGCTCTTTGTGATCTAGCCCTTAGATTTGGCGAAGGCGATGAGGAGTCCATGTCTCGACCATTTGGTGTAGCTCTTCTCATTGCCGGCCATGATGAGAAGGGGCCCAGCTTATTCTACACTGATCCATCTGGCACTTTCTGGCAATGCAATGCTAAAGCTATCGGTTCAGGTTCTGAAGGTGCAGATAGCTCTCTGCAAGAGCAATACAACAAGGACCTAACTCTTCAAGAAGCTGAAACCATAGCACTTTCCATTCTGAAGCAAGTTATGGAAGAGAAGGTAACTCCCAACAATGTCGACATTGCGAAGGTGTCTCCAACATACCATCTGTACACCCCTGCGGAGGTGGAGGCGGTCATTAGCCGGCTATGA

Protein sequence

MNRAKMAKPKTRWTPEINPKKTPASSSCFARKESSRFLQLNRKMFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLEPSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL
Homology
BLAST of Lsi02G010530 vs. ExPASy Swiss-Prot
Match: Q9M4T8 (Proteasome subunit alpha type-5 OS=Glycine max OX=3847 GN=PAE1 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 8.5e-127
Identity = 229/237 (96.62%), Postives = 234/237 (98.73%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADA TLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGV+LLIAGHDE GPSL+YTDPSGTFWQCN KAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNGKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQ+NKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKV+PTYHLYTP+EVEAVISRL
Sbjct: 181 EQFNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVAPTYHLYTPSEVEAVISRL 237

BLAST of Lsi02G010530 vs. ExPASy Swiss-Prot
Match: Q9LSU1 (Proteasome subunit alpha type-5 OS=Oryza sativa subsp. japonica OX=39947 GN=PAE1 PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 2.5e-126
Identity = 227/237 (95.78%), Postives = 234/237 (98.73%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTK+GVVLAVEKR+TSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKDGVVLAVEKRVTSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADA TLVEHARVETQNHRFSYGEPMTVESTTQA+CDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQAICDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGV+LLIAGHDE GPSL+YTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQYNK+LTLQEAETIALSILKQVMEEKVTPNNVDIAKVSP YHLYTPAEVEAVI+RL
Sbjct: 181 EQYNKELTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPNYHLYTPAEVEAVIARL 237

BLAST of Lsi02G010530 vs. ExPASy Swiss-Prot
Match: Q42134 (Proteasome subunit alpha type-5-B OS=Arabidopsis thaliana OX=3702 GN=PAE2 PE=1 SV=2)

HSP 1 Score: 447.6 bits (1150), Expect = 1.0e-124
Identity = 224/237 (94.51%), Postives = 233/237 (98.31%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIG+KTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEID+HIGCAMSGLIADA TLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEG+EESMSRPFGV+LLIAGHDE GPSL+YTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQ+NKD+TLQEAETIA+SILKQVMEEKVTPNNVDIAKV+P YHLYTP EVEAVISRL
Sbjct: 181 EQFNKDITLQEAETIAVSILKQVMEEKVTPNNVDIAKVAPAYHLYTPQEVEAVISRL 237

BLAST of Lsi02G010530 vs. ExPASy Swiss-Prot
Match: O81149 (Proteasome subunit alpha type-5-A OS=Arabidopsis thaliana OX=3702 GN=PAE1 PE=1 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 4.0e-124
Identity = 223/237 (94.09%), Postives = 233/237 (98.31%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIG+KTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEID+HIGCAMSGLIADA TLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEG+EESMSRPFGV+LLIAGHDE GPSL+YTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQ+NKDL+LQEAETIA+SILKQVMEEKVTPNNVDIAKV+P YHLYTP EVEAVI+RL
Sbjct: 181 EQFNKDLSLQEAETIAVSILKQVMEEKVTPNNVDIAKVAPAYHLYTPQEVEAVIARL 237

BLAST of Lsi02G010530 vs. ExPASy Swiss-Prot
Match: Q5E987 (Proteasome subunit alpha type-5 OS=Bos taurus OX=9913 GN=PSMA5 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.6e-88
Identity = 170/238 (71.43%), Postives = 199/238 (83.61%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTR+EYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIG++T EGV LAVEKRITSPL+E
Sbjct: 1   MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLME 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSS+EKI+EID HIGCAMSGLIADA TL++ ARVETQNH F+Y E MTVES TQA+ +LA
Sbjct: 61  PSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLA 120

Query: 164 LRFGEGDEE--SMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSS 223
           L+FGE D +  +MSRPFGVALL  G DEKGP LF+ DPSGTF QC+A+AIGS SEGA SS
Sbjct: 121 LQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSS 180

Query: 224 LQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSP--TYHLYTPAEVEAVI 278
           LQE Y+K +TL+EA   +L ILKQVMEEK+   N+++A V P   +H++T  E+E VI
Sbjct: 181 LQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVI 238

BLAST of Lsi02G010530 vs. ExPASy TrEMBL
Match: A0A0A0M0T3 (Proteasome subunit alpha type OS=Cucumis sativus OX=3659 GN=Csa_1G701940 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.6e-128
Identity = 240/253 (94.86%), Postives = 244/253 (96.44%), Query Frame = 0

Query: 28  CFARKESSRFLQLNRKMFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKE 87
           CF +     F+ +  KMFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKE
Sbjct: 31  CFRQNLPGFFISI-AKMFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKE 90

Query: 88  GVVLAVEKRITSPLLEPSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYG 147
           GVVLAVEKRITSPLLEPSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYG
Sbjct: 91  GVVLAVEKRITSPLLEPSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYG 150

Query: 148 EPMTVESTTQALCDLALRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCN 207
           EPMTVESTTQALCDLALRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCN
Sbjct: 151 EPMTVESTTQALCDLALRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCN 210

Query: 208 AKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHL 267
           AKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTP+NVDIAKVSPTYHL
Sbjct: 211 AKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPSNVDIAKVSPTYHL 270

Query: 268 YTPAEVEAVISRL 281
           YTPAEVEAVISRL
Sbjct: 271 YTPAEVEAVISRL 282

BLAST of Lsi02G010530 vs. ExPASy TrEMBL
Match: A0A1S3C987 (Proteasome subunit alpha type OS=Cucumis melo OX=3656 GN=LOC103498261 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.8e-127
Identity = 236/237 (99.58%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQYNKDLTLQEAETIALSILKQVMEEKVTP+NVDIAKVSPTYHLYTPAEVEAVISRL
Sbjct: 181 EQYNKDLTLQEAETIALSILKQVMEEKVTPSNVDIAKVSPTYHLYTPAEVEAVISRL 237

BLAST of Lsi02G010530 vs. ExPASy TrEMBL
Match: A0A5A7TPN9 (Proteasome subunit alpha type OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00380 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.8e-127
Identity = 236/237 (99.58%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQYNKDLTLQEAETIALSILKQVMEEKVTP+NVDIAKVSPTYHLYTPAEVEAVISRL
Sbjct: 181 EQYNKDLTLQEAETIALSILKQVMEEKVTPSNVDIAKVSPTYHLYTPAEVEAVISRL 237

BLAST of Lsi02G010530 vs. ExPASy TrEMBL
Match: A0A6J1KLM8 (Proteasome subunit alpha type OS=Cucurbita maxima OX=3661 GN=LOC111495679 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.1e-127
Identity = 235/237 (99.16%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQYNKDLTL+EAETIALSILKQVMEEKVTPNNVDIAKV+PTYHLYTPAEVEAVISRL
Sbjct: 181 EQYNKDLTLKEAETIALSILKQVMEEKVTPNNVDIAKVAPTYHLYTPAEVEAVISRL 237

BLAST of Lsi02G010530 vs. ExPASy TrEMBL
Match: A0A6J1EGM0 (Proteasome subunit alpha type OS=Cucurbita moschata OX=3662 GN=LOC111433997 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.1e-127
Identity = 235/237 (99.16%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQYNKDLTL+EAETIALSILKQVMEEKVTPNNVDIAKV+PTYHLYTPAEVEAVISRL
Sbjct: 181 EQYNKDLTLKEAETIALSILKQVMEEKVTPNNVDIAKVAPTYHLYTPAEVEAVISRL 237

BLAST of Lsi02G010530 vs. NCBI nr
Match: KAG6583791.1 (Proteasome subunit alpha type-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 484.6 bits (1246), Expect = 5.9e-133
Identity = 249/256 (97.27%), Postives = 251/256 (98.05%), Query Frame = 0

Query: 25  SSSCFARKESSRFLQLNRKMFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLK 84
           SS C A  ESS FLQLNRKMFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLK
Sbjct: 13  SSLCVA--ESSEFLQLNRKMFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLK 72

Query: 85  TKEGVVLAVEKRITSPLLEPSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRF 144
           TKEGVVLAVEKRITSPLLEPSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRF
Sbjct: 73  TKEGVVLAVEKRITSPLLEPSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRF 132

Query: 145 SYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFW 204
           SYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFW
Sbjct: 133 SYGEPMTVESTTQALCDLALRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFW 192

Query: 205 QCNAKAIGSGSEGADSSLQEQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPT 264
           QCNAKAIGSGSEGADSSLQEQYNKDLTL+EAETIALSILKQVMEEKVTPNNVDIAKV+PT
Sbjct: 193 QCNAKAIGSGSEGADSSLQEQYNKDLTLKEAETIALSILKQVMEEKVTPNNVDIAKVAPT 252

Query: 265 YHLYTPAEVEAVISRL 281
           YHLYTPAEVEAVISRL
Sbjct: 253 YHLYTPAEVEAVISRL 266

BLAST of Lsi02G010530 vs. NCBI nr
Match: XP_038893568.1 (proteasome subunit alpha type-5 [Benincasa hispida])

HSP 1 Score: 467.2 bits (1201), Expect = 9.7e-128
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL
Sbjct: 181 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 237

BLAST of Lsi02G010530 vs. NCBI nr
Match: XP_008459031.1 (PREDICTED: proteasome subunit alpha type-5 [Cucumis melo] >XP_008459032.1 PREDICTED: proteasome subunit alpha type-5 [Cucumis melo] >XP_011660309.1 proteasome subunit alpha type-5 [Cucumis sativus] >KAA0043189.1 proteasome subunit alpha type-5 [Cucumis melo var. makuwa] >KAE8653661.1 hypothetical protein Csa_006843 [Cucumis sativus] >TYK12278.1 proteasome subunit alpha type-5 [Cucumis melo var. makuwa])

HSP 1 Score: 465.3 bits (1196), Expect = 3.7e-127
Identity = 236/237 (99.58%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQYNKDLTLQEAETIALSILKQVMEEKVTP+NVDIAKVSPTYHLYTPAEVEAVISRL
Sbjct: 181 EQYNKDLTLQEAETIALSILKQVMEEKVTPSNVDIAKVSPTYHLYTPAEVEAVISRL 237

BLAST of Lsi02G010530 vs. NCBI nr
Match: XP_022927055.1 (proteasome subunit alpha type-5 [Cucurbita moschata] >XP_022927056.1 proteasome subunit alpha type-5 [Cucurbita moschata] >XP_023001599.1 proteasome subunit alpha type-5 [Cucurbita maxima] >XP_023001600.1 proteasome subunit alpha type-5 [Cucurbita maxima] >XP_023520352.1 proteasome subunit alpha type-5 [Cucurbita pepo subsp. pepo] >XP_023520353.1 proteasome subunit alpha type-5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 464.5 bits (1194), Expect = 6.3e-127
Identity = 235/237 (99.16%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQYNKDLTL+EAETIALSILKQVMEEKVTPNNVDIAKV+PTYHLYTPAEVEAVISRL
Sbjct: 181 EQYNKDLTLKEAETIALSILKQVMEEKVTPNNVDIAKVAPTYHLYTPAEVEAVISRL 237

BLAST of Lsi02G010530 vs. NCBI nr
Match: KAG7019414.1 (Proteasome subunit alpha type-5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 463.4 bits (1191), Expect = 1.4e-126
Identity = 234/237 (98.73%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQYNKDLTL+EAETIALSILKQVMEEKVTPNNVDIAKV+PTYHLYTPAEVEAVI+RL
Sbjct: 181 EQYNKDLTLKEAETIALSILKQVMEEKVTPNNVDIAKVAPTYHLYTPAEVEAVITRL 237

BLAST of Lsi02G010530 vs. TAIR 10
Match: AT3G14290.1 (20S proteasome alpha subunit E2 )

HSP 1 Score: 447.6 bits (1150), Expect = 7.4e-126
Identity = 224/237 (94.51%), Postives = 233/237 (98.31%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIG+KTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEID+HIGCAMSGLIADA TLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEG+EESMSRPFGV+LLIAGHDE GPSL+YTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQ+NKD+TLQEAETIA+SILKQVMEEKVTPNNVDIAKV+P YHLYTP EVEAVISRL
Sbjct: 181 EQFNKDITLQEAETIAVSILKQVMEEKVTPNNVDIAKVAPAYHLYTPQEVEAVISRL 237

BLAST of Lsi02G010530 vs. TAIR 10
Match: AT1G53850.1 (20S proteasome alpha subunit E1 )

HSP 1 Score: 445.7 bits (1145), Expect = 2.8e-125
Identity = 223/237 (94.09%), Postives = 233/237 (98.31%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIG+KTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEID+HIGCAMSGLIADA TLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEG+EESMSRPFGV+LLIAGHDE GPSL+YTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQ+NKDL+LQEAETIA+SILKQVMEEKVTPNNVDIAKV+P YHLYTP EVEAVI+RL
Sbjct: 181 EQFNKDLSLQEAETIAVSILKQVMEEKVTPNNVDIAKVAPAYHLYTPQEVEAVIARL 237

BLAST of Lsi02G010530 vs. TAIR 10
Match: AT1G53850.2 (20S proteasome alpha subunit E1 )

HSP 1 Score: 445.7 bits (1145), Expect = 2.8e-125
Identity = 223/237 (94.09%), Postives = 233/237 (98.31%), Query Frame = 0

Query: 44  MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLE 103
           MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIG+KTKEGVVLAVEKRITSPLLE
Sbjct: 1   MFLTRTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLE 60

Query: 104 PSSVEKIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 163
           PSSVEKIMEID+HIGCAMSGLIADA TLVEHARVETQNHRFSYGEPMTVESTTQALCDLA
Sbjct: 61  PSSVEKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLA 120

Query: 164 LRFGEGDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQ 223
           LRFGEG+EESMSRPFGV+LLIAGHDE GPSL+YTDPSGTFWQCNAKAIGSGSEGADSSLQ
Sbjct: 121 LRFGEGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQ 180

Query: 224 EQYNKDLTLQEAETIALSILKQVMEEKVTPNNVDIAKVSPTYHLYTPAEVEAVISRL 281
           EQ+NKDL+LQEAETIA+SILKQVMEEKVTPNNVDIAKV+P YHLYTP EVEAVI+RL
Sbjct: 181 EQFNKDLSLQEAETIAVSILKQVMEEKVTPNNVDIAKVAPAYHLYTPQEVEAVIARL 237

BLAST of Lsi02G010530 vs. TAIR 10
Match: AT3G22110.1 (20S proteasome alpha subunit C1 )

HSP 1 Score: 171.8 bits (434), Expect = 7.9e-43
Identity = 96/236 (40.68%), Postives = 148/236 (62.71%), Query Frame = 0

Query: 51  YDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLEPS-SVEK 110
           YD     FSPEGRL+QVEYA+EAI    +AIG+ +K+GVVL  EK++TS LL+ S S EK
Sbjct: 5   YDSRTTIFSPEGRLYQVEYAMEAIGNAGSAIGILSKDGVVLIGEKKVTSKLLQTSTSAEK 64

Query: 111 IMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFGEG 170
           + +ID+H+ CA++G+++DA+ L+  ARV+ Q + F Y EPM VE   Q+LCD    +   
Sbjct: 65  MYKIDDHVACAVAGIMSDANILINTARVQAQRYTFMYQEPMPVEQLVQSLCDTKQGY--- 124

Query: 171 DEESMSRPFGVALLIAGHDE-KGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNK 230
            +    RPFGV+ L AG D+  G  L+ +DPSG +    A A+G+ ++ A S L++ Y  
Sbjct: 125 TQFGGLRPFGVSFLFAGWDKHHGFQLYMSDPSGNYGGWKAAAVGANNQAAQSILKQDYKD 184

Query: 231 DLTLQEAETIALSILKQVMEE-KVTPNNVDIAKVSPT------YHLYTPAEVEAVI 278
           D T +EA  +AL +L + M+   +T   +++A+V  T      YH+++P  +  ++
Sbjct: 185 DATREEAVELALKVLTKTMDSTSLTSEKLELAEVYLTPSKTVKYHVHSPESLTKLL 237

BLAST of Lsi02G010530 vs. TAIR 10
Match: AT1G79210.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.2e-38
Identity = 81/234 (34.62%), Postives = 141/234 (60.26%), Query Frame = 0

Query: 49  TEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGLKTKEGVVLAVEKRITSPLLEPSSVE 108
           ++Y   + TFSP G+L Q+E+A+ A+  G T++G+K   GVV+A EK++ S L++ +SV+
Sbjct: 4   SQYSFSLTTFSPSGKLVQIEHALTAVGSGQTSLGIKASNGVVIATEKKLPSILVDEASVQ 63

Query: 109 KIMEIDEHIGCAMSGLIADAHTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFGE 168
           KI  +  +IG   SG+  D   LV  +R + + +   Y EP+ V   TQ + + A    E
Sbjct: 64  KIQHLTPNIGTVYSGMGPDFRVLVRKSRKQAEQYLRLYKEPIPV---TQLVRETATVMQE 123

Query: 169 GDEESMSRPFGVALLIAGHDEKGPSLFYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNK 228
             +    RPFGV+LL+AG+D+KGP L+  DPSG+++   A A+G     A + L+++Y +
Sbjct: 124 FTQSGGVRPFGVSLLVAGYDDKGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTE 183

Query: 229 DLTLQEAETIALSILKQVMEEKVTPNNVDIAKV--SPTYHLYTPAEVEAVISRL 281
           D+ L +A   A+  LK+  E +++  N++I K+     + + TPAE++  ++ +
Sbjct: 184 DMELDDAIHTAILTLKEGFEGEISSKNIEIGKIGTDKVFRVLTPAEIDDYLAEV 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M4T88.5e-12796.62Proteasome subunit alpha type-5 OS=Glycine max OX=3847 GN=PAE1 PE=2 SV=1[more]
Q9LSU12.5e-12695.78Proteasome subunit alpha type-5 OS=Oryza sativa subsp. japonica OX=39947 GN=PAE1... [more]
Q421341.0e-12494.51Proteasome subunit alpha type-5-B OS=Arabidopsis thaliana OX=3702 GN=PAE2 PE=1 S... [more]
O811494.0e-12494.09Proteasome subunit alpha type-5-A OS=Arabidopsis thaliana OX=3702 GN=PAE1 PE=1 S... [more]
Q5E9871.6e-8871.43Proteasome subunit alpha type-5 OS=Bos taurus OX=9913 GN=PSMA5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M0T31.6e-12894.86Proteasome subunit alpha type OS=Cucumis sativus OX=3659 GN=Csa_1G701940 PE=3 SV... [more]
A0A1S3C9871.8e-12799.58Proteasome subunit alpha type OS=Cucumis melo OX=3656 GN=LOC103498261 PE=3 SV=1[more]
A0A5A7TPN91.8e-12799.58Proteasome subunit alpha type OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A6J1KLM83.1e-12799.16Proteasome subunit alpha type OS=Cucurbita maxima OX=3661 GN=LOC111495679 PE=3 S... [more]
A0A6J1EGM03.1e-12799.16Proteasome subunit alpha type OS=Cucurbita moschata OX=3662 GN=LOC111433997 PE=3... [more]
Match NameE-valueIdentityDescription
KAG6583791.15.9e-13397.27Proteasome subunit alpha type-5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038893568.19.7e-128100.00proteasome subunit alpha type-5 [Benincasa hispida][more]
XP_008459031.13.7e-12799.58PREDICTED: proteasome subunit alpha type-5 [Cucumis melo] >XP_008459032.1 PREDIC... [more]
XP_022927055.16.3e-12799.16proteasome subunit alpha type-5 [Cucurbita moschata] >XP_022927056.1 proteasome ... [more]
KAG7019414.11.4e-12698.73Proteasome subunit alpha type-5 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT3G14290.17.4e-12694.5120S proteasome alpha subunit E2 [more]
AT1G53850.12.8e-12594.0920S proteasome alpha subunit E1 [more]
AT1G53850.22.8e-12594.0920S proteasome alpha subunit E1 [more]
AT3G22110.17.9e-4340.6820S proteasome alpha subunit C1 [more]
AT1G79210.11.2e-3834.62N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000426Proteasome alpha-subunit, N-terminal domainSMARTSM00948Proteasome_A_N_2coord: 51..73
e-value: 7.7E-11
score: 52.0
IPR000426Proteasome alpha-subunit, N-terminal domainPFAMPF10584Proteasome_A_Ncoord: 51..73
e-value: 1.4E-13
score: 50.2
IPR000426Proteasome alpha-subunit, N-terminal domainPROSITEPS00388PROTEASOME_ALPHA_1coord: 51..73
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 44..280
e-value: 3.9E-94
score: 316.5
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 50..277
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 74..261
e-value: 7.5E-63
score: 211.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 29..280
NoneNo IPR availablePANTHERPTHR11599:SF131PROTEASOME SUBUNIT ALPHA TYPEcoord: 29..280
IPR023332Proteasome alpha-type subunitPROSITEPS51475PROTEASOME_ALPHA_2coord: 66..277
score: 83.246864
IPR033812Proteasome subunit alpha5CDDcd03753proteasome_alpha_type_5coord: 51..261
e-value: 2.27946E-155
score: 429.835

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G010530.1Lsi02G010530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019773 proteasome core complex, alpha-subunit complex
cellular_component GO:0005839 proteasome core complex