Lsi02G009780 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G009780
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionberberine bridge enzyme-like 18
Locationchr02: 9841981 .. 9843747 (+)
RNA-Seq ExpressionLsi02G009780
SyntenyLsi02G009780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTACTTATGAATTAAAAAGGAAAAAAGAAAGTTACTTCATTGAATAAATTGAAAATTAGGTTATTCCATTTGTGTATCCCTACTAATATATACTTTCCTTTATTCAACAAAAGAGTTATTTTCCTCATCACCTACAAAATTACAAATAAAGGAAATGCAAGCACTCTAGCATGTGTTTTGTGCTGAGGAGCCCCCTATATAGCAACAAGAGAGAGTTGCAAAAAATCATTTCATTTCAATTAAGACATTGTGTAAAGTTGAAGTATATTCCAACTCCAAGCAACAACCAAACCTCTTATGTAATTTTCAATATCTCAATTAAGATAAAAAAAAAATTACAGGTAGAATCATGGTTACATCCATTTTTATACTCCCACCAAATTATTCTCTGATCATAATTTTGTGTGTGTGTGTGATGAGAGTGGAATTGCACTCAAGCATTACTCATTAACATTGCCAAAAAATCGCCCTCTTTCTATTACTACGAGACTACATTCTAATACAGTTCTTCGATACAGGTTCATAAATGGAATGGAATTTTCCATGTTATTGTTTCAATCTCTCACATAATCTTTCTCCATAAGTAATATTTGGATGCATTTTAGACTACACGTATCATTTGCAGTCCATTCCATCACACAACATAAAAAAAACTAATAGATATTAAAAAAAAAAGAAAAAAAGAAAGAAGAGTTTATCGCACGGCGAAATGTGATTGGACAAATGGGATGTGCTACAAAAATATGGGTGTTGCCAAAGATCTCAAGTACTTTCATTTTATTTTCCAACACAATAATAAAAAACCATATATAAAGAGTGAAGGATTCAAAGATAGATCACATCTCCATATATCCCAAAAACCATGAAGTACTCTTCTCCATTAATCCCTCTTGAAGTTGCCTTAGTTCTTCTATCTTCATATCCATTATGGGTTGTTGCTTCTGCTGAAGAACATCAAGCCTTTCTTCAATGTCTCTCCTCAATCTCCAAAGTCATTTACACTCCCATCAACTCCTCCTATTTTTCTGTTTTGGACTTCTCCATTCAAAACCTTAGAATCTCAAAACCTGAAACCCCAAAGCCAATAGCCATCATAACACCAACACATGTTTCACAAATTCAAGTAGCTATCCTTTGCTCTAGAACTCACGGCCTTCAAATCCGAACTCGAAGCGGCGGCCATGATTTCGAGGGTTTCGTATGTCGCCCACCATCCATTCATCATCCTTGACCTAATCAATCTTAGGTCTATATCCATTGATGTCAAAAACAACACTGCATGGGTTCAATCAGGTGCAACTGTGGGTGAACTTTACTACAAAATTGCTGAGAAAAGTCGAACCTTGGCGTTTCCGGCCGGGGTTTGTCCGTCGGTCGGCATTGGTGGGTTCATCAGTGGCGGTGGATATGGGTATTTGCTGAGGAAATATGGTCTGGCTGCTGATAATGTGATAGACGCTTACTTGGTTGACGCAAATGGGGAGGTTCATGATAGAGAGTCGATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGCGGAGGGAGCTTTGGAGTTGTGGTGGCGTGGAAGCTGAAGCTGGTTTCGATTCCGACGACCATGACGATTTGCTTTTCTAATAGAACTTGGGAGGAAGGTGCAATGAAGCTAATCTATGAATGGCAATATGTTGCTGACAAATTGGATGAAAATCTATACCTTGGCATACAATTCAATGGTATTCTTTCAAACCGCTTGCGTGAGTTTTATTATTATTATTAG

mRNA sequence

ATGGTTGCCTTAGTTCTTCTATCTTCATATCCATTATGGGTTGTTGCTTCTGCTGAAGAACATCAAGCCTTTCTTCAATGTCTCTCCTCAATCTCCAAAGTCATTTACACTCCCATCAACTCCTCCTATTTTTCTGTTTTGGACTTCTCCATTCAAAACCTTAGAATCTCAAAACCTGAAACCCCAAAGCCAATAGCCATCATAACACCAACACATGTTTCACAAATTCAACTATCCTTTGCTCTAGAACTCACGGCCTTCAAATCCGAACTCGAAGCGGCGGCCATGATTTCGAGGGTTTCGTATGTCGCCCACCATCCATTCATCATCCTTGACCTAATCAATCTTAGGTCTATATCCATTGATGTCAAAAACAACACTGCATGGGTTCAATCAGGTGCAACTGTGGGTGAACTTTACTACAAAATTGCTGAGAAAAGTCGAACCTTGGCGTTTCCGGCCGGGGTTTGTCCGTCGGTCGGCATTGGTGGGTTCATCAGTGGCGGTGGATATGGGTATTTGCTGAGGAAATATGGTCTGGCTGCTGATAATGTGATAGACGCTTACTTGGTTGACGCAAATGGGGAGGTTCATGATAGAGAGTCGATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGCGGAGGGAGCTTTGGAGTTGTGGTGGCGTGGAAGCTGAAGCTGGTTTCGATTCCGACGACCATGACGATTTGCTTTTCTAATAGAACTTGGGAGGAAGGTGCAATGAAGCTAATCTATGAATGGCAATATGTTGCTGACAAATTGGATGAAAATCTATACCTTGGCATACAATTCAATGGTATTCTTTCAAACCGCTTGCGTGAGTTTTATTATTATTATTAG

Coding sequence (CDS)

ATGGTTGCCTTAGTTCTTCTATCTTCATATCCATTATGGGTTGTTGCTTCTGCTGAAGAACATCAAGCCTTTCTTCAATGTCTCTCCTCAATCTCCAAAGTCATTTACACTCCCATCAACTCCTCCTATTTTTCTGTTTTGGACTTCTCCATTCAAAACCTTAGAATCTCAAAACCTGAAACCCCAAAGCCAATAGCCATCATAACACCAACACATGTTTCACAAATTCAACTATCCTTTGCTCTAGAACTCACGGCCTTCAAATCCGAACTCGAAGCGGCGGCCATGATTTCGAGGGTTTCGTATGTCGCCCACCATCCATTCATCATCCTTGACCTAATCAATCTTAGGTCTATATCCATTGATGTCAAAAACAACACTGCATGGGTTCAATCAGGTGCAACTGTGGGTGAACTTTACTACAAAATTGCTGAGAAAAGTCGAACCTTGGCGTTTCCGGCCGGGGTTTGTCCGTCGGTCGGCATTGGTGGGTTCATCAGTGGCGGTGGATATGGGTATTTGCTGAGGAAATATGGTCTGGCTGCTGATAATGTGATAGACGCTTACTTGGTTGACGCAAATGGGGAGGTTCATGATAGAGAGTCGATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGCGGAGGGAGCTTTGGAGTTGTGGTGGCGTGGAAGCTGAAGCTGGTTTCGATTCCGACGACCATGACGATTTGCTTTTCTAATAGAACTTGGGAGGAAGGTGCAATGAAGCTAATCTATGAATGGCAATATGTTGCTGACAAATTGGATGAAAATCTATACCTTGGCATACAATTCAATGGTATTCTTTCAAACCGCTTGCGTGAGTTTTATTATTATTATTAG

Protein sequence

MVALVLLSSYPLWVVASAEEHQAFLQCLSSISKVIYTPINSSYFSVLDFSIQNLRISKPETPKPIAIITPTHVSQIQLSFALELTAFKSELEAAAMISRVSYVAHHPFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNGILSNRLREFYYYY
Homology
BLAST of Lsi02G009780 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.1e-74
Identity = 142/275 (51.64%), Postives = 192/275 (69.82%), Query Frame = 0

Query: 6   LLSSYPLWVVASAEEHQAFLQCLS-------SISKVIYTPINSSYFSVLDFSIQNLRISK 65
           LL S P  V A+     +FLQCLS        +SKVI+TP ++S+ SVL  SIQN R S 
Sbjct: 19  LLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSA 78

Query: 66  PETPKPIAIITPTHVSQIQ--LSFALELTAFKSELEAAAMISRVSYVAHHPFIILDLINL 125
           P+ PKP+ I+TP   S +Q  +  A                  +SYV H PF+ILDL NL
Sbjct: 79  PDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNL 138

Query: 126 RSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLR 185
           RSI++DV N + WVQ+GAT+GELYY+I +K+RTLAFPAGVCP+VG+GG  SGGGYG LLR
Sbjct: 139 RSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLR 198

Query: 186 KYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTM 245
           K+GLAAD+VIDA +VDA G + +R  MGED FWAIRGGGG SF VV++WK+ L+++P+T+
Sbjct: 199 KHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTV 258

Query: 246 TICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGI 272
           T+    +  E+ A+K+I+ WQ+VADK+ ++L++ +
Sbjct: 259 TVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRV 293

BLAST of Lsi02G009780 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.1e-71
Identity = 142/278 (51.08%), Postives = 197/278 (70.86%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASAEEHQAFLQCLS-------SISKVIYTPINSSYFSVLDFSIQNL 61
           + L  L+  PL V A    H+ FL+CLS          KVI+T  +SS+FS+LD SIQN 
Sbjct: 10  ILLFSLNISPL-VSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNP 69

Query: 62  RISKPETPKPIAIITPTHVSQIQLSF-ALELTAFKSELEAAA-MISRVSYVAHH-PFIIL 121
           R S  ETPKP++IITP   S +Q      +L        +A      +SY+A++ PF ++
Sbjct: 70  RFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVI 129

Query: 122 DLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGY 181
           DL NLRSIS+DV N T WVQ+GAT GELYY+I + +++LAFPAG+ P+VG+GG  SGGGY
Sbjct: 130 DLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGY 189

Query: 182 GYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVS 241
           G LLRKYGLAADN+IDA +VDA+G + DR++MGED FWAIRGGGG SFGV+++WK+KLV 
Sbjct: 190 GTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVD 249

Query: 242 IPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYL 270
           +P+T+T+    +T ++ A+++I +WQY ADK+ ++L++
Sbjct: 250 VPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI 286

BLAST of Lsi02G009780 vs. ExPASy Swiss-Prot
Match: A0A2S1XB67 (O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 9.9e-70
Identity = 137/259 (52.90%), Postives = 186/259 (71.81%), Query Frame = 0

Query: 22  QAFLQCLS-------SISKVIYTPINSSYFSVLDFSIQNLRISKPETPKPIAIITPTHVS 81
           +AFL C+S       SI  +++ P NSSY SVL  +IQN R  K  +PKP+AIITP   S
Sbjct: 27  EAFLNCISNKFSLDVSILNILHVPSNSSYDSVLKSTIQNPRFLK--SPKPLAIITPVLHS 86

Query: 82  QIQLS-FALELTAFKSELEA-AAMISRVSYVAHHPFIILDLINLRSISIDVKNNTAWVQS 141
            +Q +    +    +  + +  A    +SY +  PFI+LDL NLRSIS+D+++N+AWV+S
Sbjct: 87  HVQSAVICTKQAGLQIRIRSGGADYEGLSYRSEVPFILLDLQNLRSISVDIEDNSAWVES 146

Query: 142 GATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLRKYGLAADNVIDAYLVD 201
           GAT+GE Y++IA+ S   AFPAGV  SVGIGG +S GG+G LLRKYGLAADN+IDA +VD
Sbjct: 147 GATIGEFYHEIAQNSPVHAFPAGVSSSVGIGGHLSSGGFGTLLRKYGLAADNIIDAKIVD 206

Query: 202 ANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTMTICFSNRTWEEGAMKL 261
           A G + DRESMGEDLFWAIRGGGG SFGV+V+WK+KLV +P  +T+   ++T+EEG + L
Sbjct: 207 ARGRILDRESMGEDLFWAIRGGGGASFGVIVSWKVKLVKVPPMVTVFILSKTYEEGGLDL 266

Query: 262 IYEWQYVADKLDENLYLGI 272
           +++WQY+  KL E+L+L +
Sbjct: 267 LHKWQYIEHKLPEDLFLAV 283

BLAST of Lsi02G009780 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 9.2e-68
Identity = 134/271 (49.45%), Postives = 188/271 (69.37%), Query Frame = 0

Query: 7   LSSYPLWVVASAEEHQAFLQCLS-----SISKVIYTPINSSYFSVLDFSIQNLRISKPET 66
           LS +P  + A    H+ FL+CLS       S++I+T  + SYFS+L+ SIQN R    ET
Sbjct: 21  LSHFPS-ISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLET 80

Query: 67  PKPIAIITPTHVSQIQLSF-ALELTAFKSELEAAAM-ISRVSYVA-HHPFIILDLINLRS 126
           PKP++IITP   + +Q +     L        +       +SY+A   PF+++DL NLRS
Sbjct: 81  PKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRS 140

Query: 127 ISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLRKY 186
           I++DV N T WVQSGAT+GELYY+I + S++LAFPAG+ P+VGIGG   GGGYG L+RKY
Sbjct: 141 ITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKY 200

Query: 187 GLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTMTI 246
           GL+ADNVIDA++VDANG   DR+ MGED FWAIRGGGG SF VV++WK++L+ +P+ +T+
Sbjct: 201 GLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTV 260

Query: 247 CFSNRTWEEGAMKLIYEWQYVADKLDENLYL 270
               +T E+ A+ +I +WQY+ADK+  +L++
Sbjct: 261 FKVVKTSEKEAVSIINKWQYIADKVPNDLFI 290

BLAST of Lsi02G009780 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 9.6e-65
Identity = 130/281 (46.26%), Postives = 187/281 (66.55%), Query Frame = 0

Query: 3   ALVLLSSYPLWV---VASAEEHQAFLQCLSS-------ISKVIYTPINSSYFSVLDFSIQ 62
           AL+L+  + +++    +S+   + F QCL+S       IS  I+   N SY SVL  +I+
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 63  NLRISKPETPKPIAIITPTHVSQIQLSFAL-ELTAFKSELEAAAM-ISRVSYVAH--HPF 122
           NLR +   TPKP  II  TH S +Q +    +    + ++ +       +SYV +   PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 123 IILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISG 182
            +LD+ NLRS+ +DV + TAWVQ+GA +GE+YY I EKS+TLA+PAG+CP+VG+GG ISG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 183 GGYGYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLK 242
           GGYG ++RKYGL  DN IDA +VD NG++ DR+ MGEDL+WAI GGGGGS+GVV+A+K+ 
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 243 LVSIPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYL 270
           LV +P  +T+   +RT E+ A  +I+ WQ VA KL + L++
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFI 284

BLAST of Lsi02G009780 vs. ExPASy TrEMBL
Match: A0A5A7TUF5 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00320 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 9.8e-121
Identity = 224/289 (77.51%), Postives = 248/289 (85.81%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASA-EEHQAFLQCLSSISKVIYTPINSSYFSVLDFSIQNLRISKPE 61
           ++ +LLS YPLWVVAS+  + QAFLQCLSSISKVIY+PINSSYFSVLDFSIQNLR SKPE
Sbjct: 12  LSFILLSPYPLWVVASSNNKPQAFLQCLSSISKVIYSPINSSYFSVLDFSIQNLRFSKPE 71

Query: 62  TPKPIAIITPTHVSQIQLSFALELTAFKSELEAAA---MISRVSYVAHHPFIILDLINLR 121
           TPKPIAIITP HVSQIQ++     T    ++   +       +SYVAHHPFIILDLINLR
Sbjct: 72  TPKPIAIITPIHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAHHPFIILDLINLR 131

Query: 122 SISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLRK 181
           SISIDVKNNTAWVQSGATVGELYYKIAEKSR+LAFPAGVCPSVGIGGF+SGGGYGYLLRK
Sbjct: 132 SISIDVKNNTAWVQSGATVGELYYKIAEKSRSLAFPAGVCPSVGIGGFVSGGGYGYLLRK 191

Query: 182 YGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTMT 241
           YGLA DNV+DAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFG+VVAWKL+LV +P T+T
Sbjct: 192 YGLAVDNVLDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAWKLRLVLVPETVT 251

Query: 242 ICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNGILSNRLREFYY 287
           IC SN+T ++GA+ LIYEWQYVADKLDENLYLGI  NGI   R    +Y
Sbjct: 252 ICISNKTVKDGAIDLIYEWQYVADKLDENLYLGILLNGISLKRFASVHY 300

BLAST of Lsi02G009780 vs. ExPASy TrEMBL
Match: A0A1S3BDG0 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488675 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.1e-119
Identity = 221/278 (79.50%), Postives = 244/278 (87.77%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASA-EEHQAFLQCLSSISKVIYTPINSSYFSVLDFSIQNLRISKPE 61
           ++ +LLS YPLWVVAS+  + QAFLQCLSSISKVIY+PINSSYFSVLDFSIQNLR SKPE
Sbjct: 12  LSFILLSPYPLWVVASSNNKPQAFLQCLSSISKVIYSPINSSYFSVLDFSIQNLRFSKPE 71

Query: 62  TPKPIAIITPTHVSQIQLSFALELTAFKSELEAAA---MISRVSYVAHHPFIILDLINLR 121
           TPKPIAIITP HVSQIQ++     T    ++   +       +SYVAHHPFIILDLINLR
Sbjct: 72  TPKPIAIITPIHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAHHPFIILDLINLR 131

Query: 122 SISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLRK 181
           SISIDVKNNTAWVQSGATVGELYYKIAEKSR+LAFPAGVCPSVGIGGF+SGGGYGYLLRK
Sbjct: 132 SISIDVKNNTAWVQSGATVGELYYKIAEKSRSLAFPAGVCPSVGIGGFVSGGGYGYLLRK 191

Query: 182 YGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTMT 241
           YGLA DNV+DAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFG+VVAWKL+LV +P T+T
Sbjct: 192 YGLAVDNVLDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAWKLRLVLVPETVT 251

Query: 242 ICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNG 276
           IC SN+T ++GA+ LIYEWQYVADKLDENLYLGI  NG
Sbjct: 252 ICISNKTVKDGAIDLIYEWQYVADKLDENLYLGILLNG 289

BLAST of Lsi02G009780 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.9e-100
Identity = 192/284 (67.61%), Postives = 226/284 (79.58%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASAEEHQAFLQCL--------SSISKVIYTPINSSYFSVLDFSIQN 61
           +A + LSS   W +A A++H++FLQCL         SISKVI+TPINSSY SVLDFSI+N
Sbjct: 14  LAFIFLSSSRFWTLAVADKHESFLQCLLNHSPDGNYSISKVIHTPINSSYSSVLDFSIRN 73

Query: 62  LRISKPETPKPIAIITPTHVSQIQLSFAL-ELTAFKSELEAAAM-ISRVSYVAHHPFIIL 121
           LR S  ETPKP+ IITP+HVS IQ +    +   F+    +      R+SYVAH  FII+
Sbjct: 74  LRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDFERLSYVAHLSFIIV 133

Query: 122 DLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGY 181
           DLINLRSIS+DVKNN AWV SGAT+GELYY IA+KSRT AFPAGVCP+VG+GG  SGGGY
Sbjct: 134 DLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVCPTVGVGGHFSGGGY 193

Query: 182 GYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVS 241
           G LLRKYGLAADNVIDAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFG+VVAWK+KLV 
Sbjct: 194 GLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVP 253

Query: 242 IPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNG 276
           +P T+T+C +NRT EEGA+KL + WQYVA+KLDENL+LGI + G
Sbjct: 254 VPATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLG 297

BLAST of Lsi02G009780 vs. ExPASy TrEMBL
Match: A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 4.3e-100
Identity = 189/285 (66.32%), Postives = 225/285 (78.95%), Query Frame = 0

Query: 4   LVLLSSYPLWVVASAEEHQAFLQCLSS-------ISKVIYTPINSSYFSVLDFSIQNLRI 63
           L+L+SS   WV AS  +HQ FLQCLS        ISKV++TPINSSY SVLDFSI+NL  
Sbjct: 14  LILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSSYSSVLDFSIRNLLF 73

Query: 64  SKPETPKPIAIITPTHVSQIQL------SFALELTAFKSELEAAAMISRVSYVAHHPFII 123
           SKPETPKP+ IITP+HVS IQ       S  L++       +       +SYVA+HPFII
Sbjct: 74  SKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD----FEGLSYVAYHPFII 133

Query: 124 LDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGG 183
           +DLINLRSI IDV+ NTAWV+SGAT+GELYY+I EKSRTLAFP GVCP+VG+GG  SGGG
Sbjct: 134 VDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVGVGGHFSGGG 193

Query: 184 YGYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLV 243
           YG +LRK+GLAADNVIDAYLVDANG+VHDR+SMGEDLFWAIRGGGGGSFG+VVAWK+KLV
Sbjct: 194 YGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLV 253

Query: 244 SIPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNG 276
           S+P T+TIC +NR  +   +KL+++WQYVA+KLDENL+LGI   G
Sbjct: 254 SVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTG 294

BLAST of Lsi02G009780 vs. ExPASy TrEMBL
Match: A0A1S3BDZ5 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 4.3e-100
Identity = 189/285 (66.32%), Postives = 225/285 (78.95%), Query Frame = 0

Query: 4   LVLLSSYPLWVVASAEEHQAFLQCLSS-------ISKVIYTPINSSYFSVLDFSIQNLRI 63
           L+L+SS   WV AS  +HQ FLQCLS        ISKV++TPINSSY SVLDFSI+NL  
Sbjct: 14  LILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSSYSSVLDFSIRNLLF 73

Query: 64  SKPETPKPIAIITPTHVSQIQL------SFALELTAFKSELEAAAMISRVSYVAHHPFII 123
           SKPETPKP+ IITP+HVS IQ       S  L++       +       +SYVA+HPFII
Sbjct: 74  SKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD----FEGLSYVAYHPFII 133

Query: 124 LDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGG 183
           +DLINLRSI IDV+ NTAWV+SGAT+GELYY+I EKSRTLAFP GVCP+VG+GG  SGGG
Sbjct: 134 VDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVGVGGHFSGGG 193

Query: 184 YGYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLV 243
           YG +LRK+GLAADNVIDAYLVDANG+VHDR+SMGEDLFWAIRGGGGGSFG+VVAWK+KLV
Sbjct: 194 YGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLV 253

Query: 244 SIPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNG 276
           S+P T+TIC +NR  +   +KL+++WQYVA+KLDENL+LGI   G
Sbjct: 254 SVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTG 294

BLAST of Lsi02G009780 vs. NCBI nr
Match: XP_004135740.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KGN66416.2 hypothetical protein Csa_023239 [Cucumis sativus])

HSP 1 Score: 451.4 bits (1160), Expect = 5.7e-123
Identity = 228/279 (81.72%), Postives = 247/279 (88.53%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASAE--EHQAFLQCLSSISKVIYTPINSSYFSVLDFSIQNLRISKP 61
           VA +LLS YPLWVVAS+   +HQAFLQCLSSI+KVIYTPIN SYFSVLDFSIQNLR SKP
Sbjct: 12  VAFILLSPYPLWVVASSNNYKHQAFLQCLSSITKVIYTPINFSYFSVLDFSIQNLRFSKP 71

Query: 62  ETPKPIAIITPTHVSQIQLSFALELTAFKSELEAAA---MISRVSYVAHHPFIILDLINL 121
           ETPKPIAIITPTHVSQIQ++     T    ++   +       +SYVAHHPFIILDLINL
Sbjct: 72  ETPKPIAIITPTHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAHHPFIILDLINL 131

Query: 122 RSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLR 181
           RSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLR
Sbjct: 132 RSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLR 191

Query: 182 KYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTM 241
           KYGLA DNVIDAYLVDANGEVHDR+SMGEDLFWAIRGGGGGSFG+VVAWKL+LVS+P T+
Sbjct: 192 KYGLAVDNVIDAYLVDANGEVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKLRLVSVPATV 251

Query: 242 TICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNG 276
           TIC SNRT ++GA+KLIYEWQYVADKLDENL+LGI  NG
Sbjct: 252 TICISNRTLKDGAIKLIYEWQYVADKLDENLHLGILLNG 290

BLAST of Lsi02G009780 vs. NCBI nr
Match: KAA0047092.1 (cannabidiolic acid synthase-like [Cucumis melo var. makuwa] >TYK05065.1 cannabidiolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 443.0 bits (1138), Expect = 2.0e-120
Identity = 224/289 (77.51%), Postives = 248/289 (85.81%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASA-EEHQAFLQCLSSISKVIYTPINSSYFSVLDFSIQNLRISKPE 61
           ++ +LLS YPLWVVAS+  + QAFLQCLSSISKVIY+PINSSYFSVLDFSIQNLR SKPE
Sbjct: 12  LSFILLSPYPLWVVASSNNKPQAFLQCLSSISKVIYSPINSSYFSVLDFSIQNLRFSKPE 71

Query: 62  TPKPIAIITPTHVSQIQLSFALELTAFKSELEAAA---MISRVSYVAHHPFIILDLINLR 121
           TPKPIAIITP HVSQIQ++     T    ++   +       +SYVAHHPFIILDLINLR
Sbjct: 72  TPKPIAIITPIHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAHHPFIILDLINLR 131

Query: 122 SISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLRK 181
           SISIDVKNNTAWVQSGATVGELYYKIAEKSR+LAFPAGVCPSVGIGGF+SGGGYGYLLRK
Sbjct: 132 SISIDVKNNTAWVQSGATVGELYYKIAEKSRSLAFPAGVCPSVGIGGFVSGGGYGYLLRK 191

Query: 182 YGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTMT 241
           YGLA DNV+DAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFG+VVAWKL+LV +P T+T
Sbjct: 192 YGLAVDNVLDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAWKLRLVLVPETVT 251

Query: 242 ICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNGILSNRLREFYY 287
           IC SN+T ++GA+ LIYEWQYVADKLDENLYLGI  NGI   R    +Y
Sbjct: 252 ICISNKTVKDGAIDLIYEWQYVADKLDENLYLGILLNGISLKRFASVHY 300

BLAST of Lsi02G009780 vs. NCBI nr
Match: XP_008445743.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo])

HSP 1 Score: 439.5 bits (1129), Expect = 2.2e-119
Identity = 221/278 (79.50%), Postives = 244/278 (87.77%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASA-EEHQAFLQCLSSISKVIYTPINSSYFSVLDFSIQNLRISKPE 61
           ++ +LLS YPLWVVAS+  + QAFLQCLSSISKVIY+PINSSYFSVLDFSIQNLR SKPE
Sbjct: 12  LSFILLSPYPLWVVASSNNKPQAFLQCLSSISKVIYSPINSSYFSVLDFSIQNLRFSKPE 71

Query: 62  TPKPIAIITPTHVSQIQLSFALELTAFKSELEAAA---MISRVSYVAHHPFIILDLINLR 121
           TPKPIAIITP HVSQIQ++     T    ++   +       +SYVAHHPFIILDLINLR
Sbjct: 72  TPKPIAIITPIHVSQIQVAIICSRTHGSLQIRTRSGGHDFEGLSYVAHHPFIILDLINLR 131

Query: 122 SISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLRK 181
           SISIDVKNNTAWVQSGATVGELYYKIAEKSR+LAFPAGVCPSVGIGGF+SGGGYGYLLRK
Sbjct: 132 SISIDVKNNTAWVQSGATVGELYYKIAEKSRSLAFPAGVCPSVGIGGFVSGGGYGYLLRK 191

Query: 182 YGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTMT 241
           YGLA DNV+DAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFG+VVAWKL+LV +P T+T
Sbjct: 192 YGLAVDNVLDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGIVVAWKLRLVLVPETVT 251

Query: 242 ICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNG 276
           IC SN+T ++GA+ LIYEWQYVADKLDENLYLGI  NG
Sbjct: 252 ICISNKTVKDGAIDLIYEWQYVADKLDENLYLGILLNG 289

BLAST of Lsi02G009780 vs. NCBI nr
Match: XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 377.1 bits (967), Expect = 1.4e-100
Identity = 190/287 (66.20%), Postives = 228/287 (79.44%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASAEEHQAFLQCLSS-------ISKVIYTPINSSYFSVLDFSIQNL 61
           + L++L S PLW  AS +++Q FLQCLS        ISKV+Y+PINSSY SVLDFSI+NL
Sbjct: 12  LVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSYSSVLDFSIKNL 71

Query: 62  RISKPETPKPIAIITPTHVSQIQL------SFALELTAFKSELEAAAMISRVSYVAHHPF 121
           R SKP+TPKP+ IITP+HVS IQ       S  L++       +       +SYVA+ PF
Sbjct: 72  RFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHD----FEGLSYVAYRPF 131

Query: 122 IILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISG 181
           I++DLINLRSIS+D +NNTAWV+SGAT+GELYY+I EKSRTLAFPAGVCP+VGIGG  SG
Sbjct: 132 IVVDLINLRSISVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSG 191

Query: 182 GGYGYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLK 241
           GGYG +LRK+GLAADNVIDA+LVDANG+VHDRESMGEDLFWAIRGGGGGSFG+VVAWK+K
Sbjct: 192 GGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIK 251

Query: 242 LVSIPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNG 276
           LV +P T+TIC + R  EE A+KL++ WQYVA+KLDENLYLGI   G
Sbjct: 252 LVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTG 294

BLAST of Lsi02G009780 vs. NCBI nr
Match: XP_022142425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 375.6 bits (963), Expect = 4.0e-100
Identity = 192/284 (67.61%), Postives = 226/284 (79.58%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASAEEHQAFLQCL--------SSISKVIYTPINSSYFSVLDFSIQN 61
           +A + LSS   W +A A++H++FLQCL         SISKVI+TPINSSY SVLDFSI+N
Sbjct: 14  LAFIFLSSSRFWTLAVADKHESFLQCLLNHSPDGNYSISKVIHTPINSSYSSVLDFSIRN 73

Query: 62  LRISKPETPKPIAIITPTHVSQIQLSFAL-ELTAFKSELEAAAM-ISRVSYVAHHPFIIL 121
           LR S  ETPKP+ IITP+HVS IQ +    +   F+    +      R+SYVAH  FII+
Sbjct: 74  LRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDFERLSYVAHLSFIIV 133

Query: 122 DLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGY 181
           DLINLRSIS+DVKNN AWV SGAT+GELYY IA+KSRT AFPAGVCP+VG+GG  SGGGY
Sbjct: 134 DLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVCPTVGVGGHFSGGGY 193

Query: 182 GYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVS 241
           G LLRKYGLAADNVIDAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFG+VVAWK+KLV 
Sbjct: 194 GLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVP 253

Query: 242 IPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGIQFNG 276
           +P T+T+C +NRT EEGA+KL + WQYVA+KLDENL+LGI + G
Sbjct: 254 VPATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLG 297

BLAST of Lsi02G009780 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 279.3 bits (713), Expect = 3.6e-75
Identity = 142/275 (51.64%), Postives = 192/275 (69.82%), Query Frame = 0

Query: 6   LLSSYPLWVVASAEEHQAFLQCLS-------SISKVIYTPINSSYFSVLDFSIQNLRISK 65
           LL S P  V A+     +FLQCLS        +SKVI+TP ++S+ SVL  SIQN R S 
Sbjct: 19  LLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSA 78

Query: 66  PETPKPIAIITPTHVSQIQ--LSFALELTAFKSELEAAAMISRVSYVAHHPFIILDLINL 125
           P+ PKP+ I+TP   S +Q  +  A                  +SYV H PF+ILDL NL
Sbjct: 79  PDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNL 138

Query: 126 RSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLR 185
           RSI++DV N + WVQ+GAT+GELYY+I +K+RTLAFPAGVCP+VG+GG  SGGGYG LLR
Sbjct: 139 RSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLR 198

Query: 186 KYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTM 245
           K+GLAAD+VIDA +VDA G + +R  MGED FWAIRGGGG SF VV++WK+ L+++P+T+
Sbjct: 199 KHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTV 258

Query: 246 TICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGI 272
           T+    +  E+ A+K+I+ WQ+VADK+ ++L++ +
Sbjct: 259 TVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRV 293

BLAST of Lsi02G009780 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.2e-72
Identity = 142/278 (51.08%), Postives = 197/278 (70.86%), Query Frame = 0

Query: 2   VALVLLSSYPLWVVASAEEHQAFLQCLS-------SISKVIYTPINSSYFSVLDFSIQNL 61
           + L  L+  PL V A    H+ FL+CLS          KVI+T  +SS+FS+LD SIQN 
Sbjct: 10  ILLFSLNISPL-VSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNP 69

Query: 62  RISKPETPKPIAIITPTHVSQIQLSF-ALELTAFKSELEAAA-MISRVSYVAHH-PFIIL 121
           R S  ETPKP++IITP   S +Q      +L        +A      +SY+A++ PF ++
Sbjct: 70  RFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVI 129

Query: 122 DLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGY 181
           DL NLRSIS+DV N T WVQ+GAT GELYY+I + +++LAFPAG+ P+VG+GG  SGGGY
Sbjct: 130 DLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGY 189

Query: 182 GYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVS 241
           G LLRKYGLAADN+IDA +VDA+G + DR++MGED FWAIRGGGG SFGV+++WK+KLV 
Sbjct: 190 GTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVD 249

Query: 242 IPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYL 270
           +P+T+T+    +T ++ A+++I +WQY ADK+ ++L++
Sbjct: 250 VPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI 286

BLAST of Lsi02G009780 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 258.5 bits (659), Expect = 6.6e-69
Identity = 134/271 (49.45%), Postives = 188/271 (69.37%), Query Frame = 0

Query: 7   LSSYPLWVVASAEEHQAFLQCLS-----SISKVIYTPINSSYFSVLDFSIQNLRISKPET 66
           LS +P  + A    H+ FL+CLS       S++I+T  + SYFS+L+ SIQN R    ET
Sbjct: 21  LSHFPS-ISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLET 80

Query: 67  PKPIAIITPTHVSQIQLSF-ALELTAFKSELEAAAM-ISRVSYVA-HHPFIILDLINLRS 126
           PKP++IITP   + +Q +     L        +       +SY+A   PF+++DL NLRS
Sbjct: 81  PKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRS 140

Query: 127 ISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISGGGYGYLLRKY 186
           I++DV N T WVQSGAT+GELYY+I + S++LAFPAG+ P+VGIGG   GGGYG L+RKY
Sbjct: 141 ITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKY 200

Query: 187 GLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLKLVSIPTTMTI 246
           GL+ADNVIDA++VDANG   DR+ MGED FWAIRGGGG SF VV++WK++L+ +P+ +T+
Sbjct: 201 GLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTV 260

Query: 247 CFSNRTWEEGAMKLIYEWQYVADKLDENLYL 270
               +T E+ A+ +I +WQY+ADK+  +L++
Sbjct: 261 FKVVKTSEKEAVSIINKWQYIADKVPNDLFI 290

BLAST of Lsi02G009780 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 248.4 bits (633), Expect = 6.8e-66
Identity = 130/281 (46.26%), Postives = 187/281 (66.55%), Query Frame = 0

Query: 3   ALVLLSSYPLWV---VASAEEHQAFLQCLSS-------ISKVIYTPINSSYFSVLDFSIQ 62
           AL+L+  + +++    +S+   + F QCL+S       IS  I+   N SY SVL  +I+
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 63  NLRISKPETPKPIAIITPTHVSQIQLSFAL-ELTAFKSELEAAAM-ISRVSYVAH--HPF 122
           NLR +   TPKP  II  TH S +Q +    +    + ++ +       +SYV +   PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 123 IILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFISG 182
            +LD+ NLRS+ +DV + TAWVQ+GA +GE+YY I EKS+TLA+PAG+CP+VG+GG ISG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 183 GGYGYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWKLK 242
           GGYG ++RKYGL  DN IDA +VD NG++ DR+ MGEDL+WAI GGGGGS+GVV+A+K+ 
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 243 LVSIPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYL 270
           LV +P  +T+   +RT E+ A  +I+ WQ VA KL + L++
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFI 284

BLAST of Lsi02G009780 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.3e-64
Identity = 125/285 (43.86%), Postives = 186/285 (65.26%), Query Frame = 0

Query: 5   VLLSSYPLWVVASAEEHQAFLQCLSSISKVIYTPINSSYFS----------VLDFSIQNL 64
           +LL S PL   +S+   Q F++CL   S V + PI +S+FS           L+ + QNL
Sbjct: 17  LLLISVPL---SSSTLQQDFVKCLVDNSDVSF-PITASFFSPDQNATLFKEELESTAQNL 76

Query: 65  RISKPETPKPIAIITPTHVSQIQ--------LSFALELTAFKSELEAAAMISRVSYVAHH 124
           R   P  PKP+ I  P + + +Q        L   L L +   + E  + ++        
Sbjct: 77  RYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAE----DET 136

Query: 125 PFIILDLINLRSISIDVKNNTAWVQSGATVGELYYKIAEKSRTLAFPAGVCPSVGIGGFI 184
           PF+I+DL  LR + +D+ +N+AW  +GAT+GE+YY+I EKS+T  FPAG+C S+GIGG +
Sbjct: 137 PFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHL 196

Query: 185 SGGGYGYLLRKYGLAADNVIDAYLVDANGEVHDRESMGEDLFWAIRGGGGGSFGVVVAWK 244
            GG YG ++RK+GL ADNV+DA +VDANG++ DR +MGED+FWAIRGGGGGSFGV++AWK
Sbjct: 197 VGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWK 256

Query: 245 LKLVSIPTTMTICFSNRTWEEGAMKLIYEWQYVADKLDENLYLGI 272
           +KLV +P T+T+    +T E+   K++Y+W+ +ADKLD++L++ +
Sbjct: 257 IKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRV 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SVG55.1e-7451.64Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... [more]
Q9FI213.1e-7151.08Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=... [more]
A0A2S1XB679.9e-7052.90O-acetylstemmadenine oxidase OS=Catharanthus roseus OX=4058 GN=ASO PE=1 SV=1[more]
Q9FI259.2e-6849.45Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=... [more]
Q9SA859.6e-6546.26Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7TUF59.8e-12177.51Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BDG01.1e-11979.50cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488675 PE=3 SV... [more]
A0A6J1CKW81.9e-10067.61berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 P... [more]
A0A5D3C1A14.3e-10066.32Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BDZ54.3e-10066.32tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10348863... [more]
Match NameE-valueIdentityDescription
XP_004135740.25.7e-12381.72berberine bridge enzyme-like 18 [Cucumis sativus] >KGN66416.2 hypothetical prote... [more]
KAA0047092.12.0e-12077.51cannabidiolic acid synthase-like [Cucumis melo var. makuwa] >TYK05065.1 cannabid... [more]
XP_008445743.12.2e-11979.50PREDICTED: cannabidiolic acid synthase-like [Cucumis melo][more]
XP_038895543.11.4e-10066.20berberine bridge enzyme-like 18 [Benincasa hispida][more]
XP_022142425.14.0e-10067.61berberine bridge enzyme-like 18 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G20820.13.6e-7551.64FAD-binding Berberine family protein [more]
AT5G44440.12.2e-7251.08FAD-binding Berberine family protein [more]
AT5G44410.16.6e-6949.45FAD-binding Berberine family protein [more]
AT1G30700.16.8e-6646.26FAD-binding Berberine family protein [more]
AT2G34790.11.3e-6443.86FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 18..116
e-value: 3.3E-17
score: 64.3
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 120..234
e-value: 6.4E-74
score: 251.5
NoneNo IPR availableGENE3D3.40.462.20coord: 235..274
e-value: 6.4E-74
score: 251.5
NoneNo IPR availablePANTHERPTHR32448:SF142CANNABIDIOLIC ACID SYNTHASE-LIKEcoord: 4..276
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 4..276
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 107..198
e-value: 3.4E-20
score: 72.2
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 60..232
score: 15.149248
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 13..233

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G009780.1Lsi02G009780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding