Lsi02G009670 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G009670 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 9.3e-180
Identity = 321/531 (60.45%), Postives = 396/531 (74.58%), Query Frame = 0

Query: 4   LRYSILLLSFSFLVFF---SSATTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 63
           ++Y+++L+ F F+VF    SS++   E F QCL  +S P +PI+PAIF   N SYSSVL+
Sbjct: 1   MKYALILVLF-FVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQ 60

Query: 64  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYIS-D 123
             IRNLRFNT++TPKPFLI+ A HESHV+ AI C + HNLQMKIRSGGHDY+G+SY++  
Sbjct: 61  ANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYS 120

Query: 124 VIPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGG 183
             PFFVLDMFNLR + VDV ++TAWVQTGA LGEVYY I EK+K+  +PAG+CPTVGVGG
Sbjct: 121 GKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGG 180

Query: 184 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 243
           H  GGGYGNMMRKYGL+VD  IDA++VDVNGK+LDRK MGEDL+WAI GGGG S+GVV+A
Sbjct: 181 HISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLA 240

Query: 244 YKIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGK 303
           YKI  V VPE VTVF++ RTLE+N  T+I+  WQ+VA  +  +LFIR   DVVNGT   +
Sbjct: 241 YKINLVEVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQ 300

Query: 304 KTLRATFIALFLGDSERLLSVINKSFPKLGT-----------------------SVDALL 363
           KT+R TFIA+FLGD+  LLS++N+ FP+LG                        S + LL
Sbjct: 301 KTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLL 360

Query: 364 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 423
            ++ Q + +LKRKSDYV+  I + GL  IWKKMIELE P + FNPYGG M  I S  TPF
Sbjct: 361 LQRNQPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPF 420

Query: 424 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 483
           P+RAGNLWKIQY  NW +E     + +++LTRKL++FMTPFVSKNPR +F NYRD+D+GI
Sbjct: 421 PYRAGNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGI 480

Query: 484 N-HNGK-NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFP 506
           N HNGK +SY EG+ YG KYF GNF+RLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 481 NSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Lsi02G009670 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 3.3e-153
Identity = 285/519 (54.91%), Postives = 358/519 (68.98%), Query Frame = 0

Query: 16  LVFFSSAT-----TAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRNLRFN 75
           L FFSSA      +  E+F QC   +   P   I   +F+  N S+SSVL  YIRN RFN
Sbjct: 20  LPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNGRFN 79

Query: 76  TSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDVIPFFVLDMF 135
           TS+TPKP +I+T   + HV  A+ C++  N  +KIRSGGHDYEG+SYISD  PFF+LDM 
Sbjct: 80  TSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDK-PFFILDMS 139

Query: 136 NLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGHFGGGGYGNM 195
           NLRD++VD+  ++AW+  GATLGEVYYRI EK+K  GFPAGVCPTVGVGGH  GGGYGNM
Sbjct: 140 NLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGYGNM 199

Query: 196 MRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRAVHVPE 255
           +RK+GLSVD +IDAKIVDVNG++LDRK+MGEDLFWAI GGGG+SFGVV+ YK++ V VPE
Sbjct: 200 LRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVPVPE 259

Query: 256 TVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKKTLRATFIAL 315
           TVTVF+VE+ ++   V ++V +WQ V    D++LF+R+    V  T K  KT+RAT +AL
Sbjct: 260 TVTVFRVEKYMDSGAV-DMVHKWQSVGPKTDRNLFLRMLIQPV--TRKKVKTVRATVVAL 319

Query: 316 FLGDSERLLSVINKSFPKLG------------------------TSVD--ALLSRKPQVL 375
           FLG +E +++++ K FP+L                         T +D    L R     
Sbjct: 320 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 379

Query: 376 THLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAGNL 435
              KRKSDYV + IP++G+  ++KKM EL K  L FNPYGG+MAE+  NATPFPHR+  L
Sbjct: 380 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 439

Query: 436 WKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGINHNGKNS 495
           +KIQY+  W E        F++    L+ FMT FVSKNPR A+LNYRD+DIG+N +G NS
Sbjct: 440 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNS 499

Query: 496 YYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIP 503
           Y EG VYG KYF  NFDRLVK+KT  DPDNFFRNEQSIP
Sbjct: 500 YEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of Lsi02G009670 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 531.6 bits (1368), Expect = 1.0e-149
Identity = 278/524 (53.05%), Postives = 358/524 (68.32%), Query Frame = 0

Query: 13  FSFLVF-------FSSATTAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIR 72
           F FL+F       FS + +   +F +C   +   P   I   +F+  N ++SSVL  YIR
Sbjct: 16  FFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIR 75

Query: 73  NLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDVIPFF 132
           N RFNTS+T KP +I+T   ESHV  A+ C++  N  +KIRSGGHDY+G+SYISD  PFF
Sbjct: 76  NARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK-PFF 135

Query: 133 VLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGHFGGG 192
           +LDM N+RD++VD+ + +AW+  GATLGEVYYRI EK++  GFPAGVCPTVGVGGH  GG
Sbjct: 136 ILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGG 195

Query: 193 GYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRA 252
           GYGNM+RK+GLSVD + DAKIVDVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK++ 
Sbjct: 196 GYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKL 255

Query: 253 VHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKKTLRA 312
           V VP  VTVF+VE+ ++   V ++V +WQ V    D +LF+R+    V  T K  KT+RA
Sbjct: 256 VPVPSVVTVFRVEQYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRA 315

Query: 313 TFIALFLGDSERLLSVINKSFPKLG------------------------TSVD--ALLSR 372
           + +ALFLG ++ ++++++K FP+LG                        T VD    L R
Sbjct: 316 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 375

Query: 373 KPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPH 432
                +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPH
Sbjct: 376 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 435

Query: 433 RAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGINH 492
           R   L+KIQY+ NW E        +++  + L+ FMT FVSKNPR+++ NYRD+DIG+N 
Sbjct: 436 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 495

Query: 493 NGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIP 503
           +G NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 496 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Lsi02G009670 vs. ExPASy Swiss-Prot
Match: Q9SA99 (Berberine bridge enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11770 PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 4.2e-148
Identity = 279/529 (52.74%), Postives = 352/529 (66.54%), Query Frame = 0

Query: 8   ILLLSFSFLVFFSSATTAPE----NFFQCLLRHS-PPSYPIAPAIFTPNNASYSSVLETY 67
           IL L  SF+    +    PE    NF QC    +  P   +A  +     A+++ VL  Y
Sbjct: 7   ILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRAY 66

Query: 68  IRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDVIP 127
           IRN RFNT+ TPKP +++ A  ESHV+ A++C +  N+Q+K RSGGHDYEGVSYIS V P
Sbjct: 67  IRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISHV-P 126

Query: 128 FFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGHFG 187
           FFVLDM NLR+ITVD  TE+AWV  GATLGEVYYRI EK KS GFPAGVCPTVG GGH  
Sbjct: 127 FFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHIS 186

Query: 188 GGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKI 247
           GGGYGNM+RKYGLSVD + DAKIVDVNG++LDRK MGED+FWAI GGGG+SFGV++A+KI
Sbjct: 187 GGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFKI 246

Query: 248 RAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKKTL 307
           + V VP TVTVF+VE+ L +N  TE+V +WQ VA   D  LF+R+    V  T    +T+
Sbjct: 247 KLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQTV 306

Query: 308 RATFIALFLGDSERLLSVINKSFPKLG--------------------------TSVDALL 367
           RA+ +ALFLGD   ++S++ K FP+LG                             + LL
Sbjct: 307 RASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEILL 366

Query: 368 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 427
            R P + T  KRKSD+V+  I K+GL+ ++KKMIE+ K  L FNPYGG M+ + +  TPF
Sbjct: 367 DRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTPF 426

Query: 428 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 487
           PHR   L+KIQ++ NW + GT     F+   +  + +M PFV+KNPR  ++NYRDLDIG+
Sbjct: 427 PHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIGV 486

Query: 488 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFP 506
           N  G NSY    V+G  YF  NFDRLVK+KT VDP NFFR+EQSIP  P
Sbjct: 487 NTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

BLAST of Lsi02G009670 vs. ExPASy Swiss-Prot
Match: Q9LPC3 (Berberine bridge enzyme-like 1 OS=Arabidopsis thaliana OX=3702 GN=SEC1A PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.7e-144
Identity = 276/535 (51.59%), Postives = 357/535 (66.73%), Query Frame = 0

Query: 8   ILLLSFSFLVFFSSATTAP-----ENFFQCLLRHS-PPSYPIAPAIFTPNNASYSSVLET 67
           ++ L  S LV  S AT  P     E+F QC    +  P   +   +   ++AS++  L  
Sbjct: 6   LVFLIVSSLVSSSLATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRA 65

Query: 68  YIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDVI 127
           YIRN RFNTST+PKP L++ A  E HV+  +LC +  N Q+K RSGGHDY+GVSYIS+  
Sbjct: 66  YIRNARFNTSTSPKPLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISN-R 125

Query: 128 PFFVLDMFNLRDITVDVKTE--TAWVQTGATLGEVYYRIAEKNK---SFGFPAGVCPTVG 187
           PFFVLDM  LR+ITVD+  +  +AWV  GATLGEVYY I + +K   + GFPAGVCPTVG
Sbjct: 126 PFFVLDMSYLRNITVDMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVG 185

Query: 188 VGGHFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGV 247
            GGH  GGGYGNM+RKYGLSVD + DAKIVDVNG++LDRK+MGEDLFWAI GGGG+SFGV
Sbjct: 186 AGGHISGGGYGNMIRKYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIGGGGGASFGV 245

Query: 248 VVAYKIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTD 307
           ++++KI+ V VP  VTVF+VE+TL +N   ++V +WQ VA     DLF+R+    V  T 
Sbjct: 246 ILSFKIKLVPVPPRVTVFRVEKTLVEN-ALDMVHKWQFVAPKTSPDLFMRLMLQPV--TR 305

Query: 308 KGKKTLRATFIALFLGDSERLLSVINKSFPKLG--------------------------T 367
              +T+RA+ +ALFLG    L+S++ K FP+LG                           
Sbjct: 306 NTTQTVRASVVALFLGKQSDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVI 365

Query: 368 SVDALLSRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIP 427
             + LL R P   + LKRKSDYV+  I K+GL+ + KK++E  K  L FNPYGG+M+E+ 
Sbjct: 366 KPEILLDRNPDSASFLKRKSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVA 425

Query: 428 SNATPFPHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYR 487
           + ATPFPHR   L+K+Q++ NW + GT   + F++ TR  + +M PFV+KNPR  +LNYR
Sbjct: 426 TTATPFPHRK-RLFKVQHSMNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYR 485

Query: 488 DLDIGINHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFP 506
           DLDIGIN +G NSY E  VYG KYF  NFDRLVK+KT VDP+NFFR+EQSIP  P
Sbjct: 486 DLDIGINSHGPNSYREAEVYGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTLP 535

BLAST of Lsi02G009670 vs. ExPASy TrEMBL
Match: A0A5A7TVZ2 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold466G001070 PE=3 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 1.9e-268
Identity = 466/532 (87.59%), Postives = 488/532 (91.73%), Query Frame = 0

Query: 1   MGILRYSILLLSFSFLVFFSSA-TTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M ILR SIL LSFSFLVFFSSA ++APENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSY+SD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VIPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGG 180
           V+PFFVLDMFNLR+ITVD KTETAWVQTGATLGEVYYR+AEKNKS+GFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGK 300
           YKIR V VPE VTVF+V+RTLE+N++TEIVDEWQ+VAHVID DLFIRVTFDVVNGTDK K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSERLLSVINKSFPK------------------------LGTSVDAL 360
           KTLRATFIALFLGDS RLL VIN SFPK                        LGTSV+AL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSR PQVLTHLKRKSDYVK  IPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDEEG R+A HFIDLTRKL+KFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 INHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHV 508
           +NHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Lsi02G009670 vs. ExPASy TrEMBL
Match: A0A1S3BE21 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 1.9e-268
Identity = 466/532 (87.59%), Postives = 488/532 (91.73%), Query Frame = 0

Query: 1   MGILRYSILLLSFSFLVFFSSA-TTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M ILR SIL LSFSFLVFFSSA ++APENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSY+SD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VIPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGG 180
           V+PFFVLDMFNLR+ITVD KTETAWVQTGATLGEVYYR+AEKNKS+GFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGK 300
           YKIR V VPE VTVF+V+RTLE+N++TEIVDEWQ+VAHVID DLFIRVTFDVVNGTDK K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSERLLSVINKSFPK------------------------LGTSVDAL 360
           KTLRATFIALFLGDS RLL VIN SFPK                        LGTSV+AL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSR PQVLTHLKRKSDYVK  IPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDEEG R+A HFIDLTRKL+KFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 INHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHV 508
           +NHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Lsi02G009670 vs. ExPASy TrEMBL
Match: A0A6J1GSQ1 (berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.5e-268
Identity = 466/533 (87.43%), Postives = 486/533 (91.18%), Query Frame = 0

Query: 1   MGILRYSILLLSFSFLVFFSSATTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 60
           M I R+S  LLSFS L+FF+SATTAPENF QCLLRHSPPSYPIAPAIFT NNASYSSVLE
Sbjct: 3   MDISRFSSFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDV 120
           TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSY+SDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 IPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGH 180
           +PFFVLDMFNLRDI+VDVKTETAWVQTGATLGEVYYRIAEK K  GFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDVNG LLDRKAMGEDLFWAIIGGGGSSFGVVVAY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKK 300
           KIR V VPETVTVF+V RTLE NDVTEIVD+WQRVAHVIDKDLFIRVTFDVVNG+  GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGKK 302

Query: 301 TLRATFIALFLGDSERLLSVINKSFPKL------------------------GTSVDALL 360
           TLRATFIALFLGDSERLLSV+NKSFPKL                        GTSVD LL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKADCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYVKTLIPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNWDE+GT+QANHFIDLTRKL+KFMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHVGR 510
           NHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDP NFFRNEQSIPRFP+VGR
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYVGR 535

BLAST of Lsi02G009670 vs. ExPASy TrEMBL
Match: A0A6J1K3U3 (berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 9.4e-268
Identity = 466/533 (87.43%), Postives = 486/533 (91.18%), Query Frame = 0

Query: 1   MGILRYSILLLSFSFLVFFSSATTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 60
           M I R+SI LLSFS L+FF+SATTAPENF QCLLRHSPPSYPIAPAIFT NNASYSSVLE
Sbjct: 3   MEISRFSIFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDV 120
           TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSY+SDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 IPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGH 180
           +PFFVLDMFNLRDI+VDVKTETAWVQTGATLGEVYYRIAEK K  GFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDV G LLDRKAMGEDLFWAIIGGGGSSFGVVVAY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKK 300
           KIR V VPETVTVF+V RTLE NDVTEIVD+WQRVAHVIDKDLFIRVTFDVVNG+  GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGKK 302

Query: 301 TLRATFIALFLGDSERLLSVINKSFPKL------------------------GTSVDALL 360
           TLRATFIALFLGDSERLLSV+NKSFPKL                        GTSVD LL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYVKTLIPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNWDE+GT+QANHFIDLTRKL+KFMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHVGR 510
           NHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDP NFFRNEQSIPRFP+VGR
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYVGR 535

BLAST of Lsi02G009670 vs. ExPASy TrEMBL
Match: A0A6J1DGF3 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020214 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 9.4e-252
Identity = 441/533 (82.74%), Postives = 476/533 (89.31%), Query Frame = 0

Query: 3   ILRYSILLLSFSFLVFFSSATT--APENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 62
           ILR   LL     L+FF+SATT  +PENF +CLLR SPPS+PI+PAIFTP+NAS+SSVL+
Sbjct: 5   ILRPFFLLSLLFSLLFFASATTTASPENFLKCLLRRSPPSHPISPAIFTPDNASFSSVLQ 64

Query: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDV 122
           TYIRNLRFNTS+TPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSY+SDV
Sbjct: 65  TYIRNLRFNTSSTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDV 124

Query: 123 IPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGH 182
           +PFFVLDMFNLR ITVDV+TETAWV+TGATLGEVYYRIAEK+K  GFPAGVCPTVGVGGH
Sbjct: 125 VPFFVLDMFNLRAITVDVETETAWVETGATLGEVYYRIAEKSKLHGFPAGVCPTVGVGGH 184

Query: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242
           FGGGGYGNMMRKYGLSVDQIIDAK+VDVNGKLLDRK+MGEDLFWAI GGGGSSFG VVAY
Sbjct: 185 FGGGGYGNMMRKYGLSVDQIIDAKMVDVNGKLLDRKSMGEDLFWAITGGGGSSFGXVVAY 244

Query: 243 KIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKK 302
           KI+ V VPETVTVFKVER L +N VTEIVDEWQRVAH ID DLFIRVTFDVVNGT+ GKK
Sbjct: 245 KIQTVRVPETVTVFKVERRLAEN-VTEIVDEWQRVAHEIDNDLFIRVTFDVVNGTENGKK 304

Query: 303 TLRATFIALFLGDSERLLSVINKSFPK------------------------LGTSVDALL 362
           TLRATFIALFLGDSERLLS+++KSFPK                        LGTSVDALL
Sbjct: 305 TLRATFIALFLGDSERLLSIMDKSFPKLGLKQSDCLQMSWLESVLFWTNFPLGTSVDALL 364

Query: 363 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 422
           SRKPQVLTHLKRKSDYVKTLIPKEGLN+IWKKMIELEKPMLTFNPYGGRMAEI S+ TPF
Sbjct: 365 SRKPQVLTHLKRKSDYVKTLIPKEGLNQIWKKMIELEKPMLTFNPYGGRMAEIASSKTPF 424

Query: 423 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 482
           PHRAGNLWKIQYATNWDEEG  +A+HFI+LTRKL+ FMTPFVSKNPRTAFLNYRDLDIG+
Sbjct: 425 PHRAGNLWKIQYATNWDEEGVEEADHFINLTRKLYNFMTPFVSKNPRTAFLNYRDLDIGV 484

Query: 483 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHVGR 510
           NHNGKNSY EGRV+G+KYFEGNFDRLVKIKT+VDP+NFFRNEQSIPR P+V R
Sbjct: 485 NHNGKNSYNEGRVFGVKYFEGNFDRLVKIKTRVDPNNFFRNEQSIPRLPYVKR 536

BLAST of Lsi02G009670 vs. NCBI nr
Match: XP_038895093.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 963.4 bits (2489), Expect = 7.9e-277
Identity = 476/533 (89.31%), Postives = 498/533 (93.43%), Query Frame = 0

Query: 1   MGILRYSILLLSFSFLVFFSSATTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 60
           M ILR SILLLSFS+LVFFSSAT+APENFFQCLLRHSPPSYPIAPAIFTPNN S+SSVLE
Sbjct: 1   MEILRSSILLLSFSYLVFFSSATSAPENFFQCLLRHSPPSYPIAPAIFTPNNVSFSSVLE 60

Query: 61  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDV 120
           TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSY+SDV
Sbjct: 61  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 120

Query: 121 IPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGH 180
           +PFF+LDMFNLRDITVDVKT+TAWVQTGATLGEVYYRIAEKNK +GFPAGVCPTVGVGGH
Sbjct: 121 LPFFILDMFNLRDITVDVKTKTAWVQTGATLGEVYYRIAEKNKFYGFPAGVCPTVGVGGH 180

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY
Sbjct: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 240

Query: 241 KIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKK 300
           KIR V VPETVTVF+V+RTLE+ND+TEIVDEWQRVAHVID DLFIRVTFDV+NGT+KGKK
Sbjct: 241 KIRMVRVPETVTVFRVQRTLEQNDLTEIVDEWQRVAHVIDNDLFIRVTFDVINGTEKGKK 300

Query: 301 TLRATFIALFLGDSERLLSVINKSFPK------------------------LGTSVDALL 360
           TLRATF+A+FLGDSERLLSVI KSFPK                        LGTSVDALL
Sbjct: 301 TLRATFLAMFLGDSERLLSVIKKSFPKLGLKKSDCLEMSWLQSVLFWTNFPLGTSVDALL 360

Query: 361 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYVKT IPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF
Sbjct: 361 SRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 420

Query: 421 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNWDEEGT++A HFIDLTRKL+KFMTPFVSKNPRTAFLNYRDLDIG+
Sbjct: 421 PHRAGNLWKIQYATNWDEEGTQEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGV 480

Query: 481 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHVGR 510
           NHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPDNFFRNEQSIPRFPHVGR
Sbjct: 481 NHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHVGR 533

BLAST of Lsi02G009670 vs. NCBI nr
Match: XP_004135723.1 (berberine bridge enzyme-like 8 [Cucumis sativus] >KAE8653304.1 hypothetical protein Csa_023313 [Cucumis sativus])

HSP 1 Score: 943.7 bits (2438), Expect = 6.4e-271
Identity = 470/532 (88.35%), Postives = 491/532 (92.29%), Query Frame = 0

Query: 1   MGILRYSILLLSFSFLVFFSSA-TTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M ILR SILLLSFSFLVFFSSA ++APENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSY+SD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VIPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGG 180
           V+PFF+LDMFNLR+ITVD KT+TAWVQTGATLGEVYYR+AE NKS+GFPAGVCPTVGVGG
Sbjct: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGK 300
           YKIR V VPETVTVF+V+RTLE+ND+TEIVDEWQ+VAHVID DLFIRVTFDVVNGT+KGK
Sbjct: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300

Query: 301 KTLRATFIALFLGDSERLLSVINKSFPK------------------------LGTSVDAL 360
           KTLRATFIALFLGDSERLL VIN SFPK                        LGTSV+AL
Sbjct: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSR PQVLTHLKRKSDYVKT IPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG ++A HFIDLTRKL+KFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 INHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHV 508
           +NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Lsi02G009670 vs. NCBI nr
Match: XP_008445642.1 (PREDICTED: reticuline oxidase-like protein [Cucumis melo] >KAA0047124.1 reticuline oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 934.5 bits (2414), Expect = 3.9e-268
Identity = 466/532 (87.59%), Postives = 488/532 (91.73%), Query Frame = 0

Query: 1   MGILRYSILLLSFSFLVFFSSA-TTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M ILR SIL LSFSFLVFFSSA ++APENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSY+SD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VIPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGG 180
           V+PFFVLDMFNLR+ITVD KTETAWVQTGATLGEVYYR+AEKNKS+GFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGK 300
           YKIR V VPE VTVF+V+RTLE+N++TEIVDEWQ+VAHVID DLFIRVTFDVVNGTDK K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSERLLSVINKSFPK------------------------LGTSVDAL 360
           KTLRATFIALFLGDS RLL VIN SFPK                        LGTSV+AL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSR PQVLTHLKRKSDYVK  IPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWDEEG R+A HFIDLTRKL+KFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 INHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHV 508
           +NHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Lsi02G009670 vs. NCBI nr
Match: KAG6573419.1 (Berberine bridge enzyme-like 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 934.1 bits (2413), Expect = 5.1e-268
Identity = 467/533 (87.62%), Postives = 486/533 (91.18%), Query Frame = 0

Query: 1   MGILRYSILLLSFSFLVFFSSATTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 60
           M ILR+S  LLSFS L+FF+SATTAPENF QCLLRHSPPSYPIAPAIFT NNASYSSVLE
Sbjct: 3   MEILRFSSFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDV 120
           TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSY+SDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 IPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGH 180
           +PFFVLDMFNLRDI+VDVKTETAWVQTGATLGEVYYRIAEK K  GFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDVNG LLDRKAMGEDLFWAIIGGGGSSFGVVVAY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKK 300
           KIR V VPETVTVF+V RTLE NDVTEIVD+WQRVAHVIDKDLFIRVTFDVVNG   GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGRRSGKK 302

Query: 301 TLRATFIALFLGDSERLLSVINKSFPKL------------------------GTSVDALL 360
           TLRATFIALFLGDSERLLSV+NKSFPKL                        GTSVD LL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYVKTLIPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNWDE+GT+QANHFIDLTRKL+KFMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHVGR 510
           NHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDP NFFRNEQSIPRFP+VGR
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYVGR 535

BLAST of Lsi02G009670 vs. NCBI nr
Match: XP_022954545.1 (berberine bridge enzyme-like 8 [Cucurbita moschata])

HSP 1 Score: 932.9 bits (2410), Expect = 1.1e-267
Identity = 466/533 (87.43%), Postives = 486/533 (91.18%), Query Frame = 0

Query: 1   MGILRYSILLLSFSFLVFFSSATTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 60
           M I R+S  LLSFS L+FF+SATTAPENF QCLLRHSPPSYPIAPAIFT NNASYSSVLE
Sbjct: 3   MDISRFSSFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDV 120
           TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIH+LQMKIRSGGHDYEGVSY+SDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 IPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGH 180
           +PFFVLDMFNLRDI+VDVKTETAWVQTGATLGEVYYRIAEK K  GFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDVNG LLDRKAMGEDLFWAIIGGGGSSFGVVVAY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKK 300
           KIR V VPETVTVF+V RTLE NDVTEIVD+WQRVAHVIDKDLFIRVTFDVVNG+  GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGKK 302

Query: 301 TLRATFIALFLGDSERLLSVINKSFPKL------------------------GTSVDALL 360
           TLRATFIALFLGDSERLLSV+NKSFPKL                        GTSVD LL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKADCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYVKTLIPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNWDE+GT+QANHFIDLTRKL+KFMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFPHVGR 510
           NHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDP NFFRNEQSIPRFP+VGR
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYVGR 535

BLAST of Lsi02G009670 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 631.3 bits (1627), Expect = 6.6e-181
Identity = 321/531 (60.45%), Postives = 396/531 (74.58%), Query Frame = 0

Query: 4   LRYSILLLSFSFLVFF---SSATTAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 63
           ++Y+++L+ F F+VF    SS++   E F QCL  +S P +PI+PAIF   N SYSSVL+
Sbjct: 1   MKYALILVLF-FVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQ 60

Query: 64  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYIS-D 123
             IRNLRFNT++TPKPFLI+ A HESHV+ AI C + HNLQMKIRSGGHDY+G+SY++  
Sbjct: 61  ANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYS 120

Query: 124 VIPFFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGG 183
             PFFVLDMFNLR + VDV ++TAWVQTGA LGEVYY I EK+K+  +PAG+CPTVGVGG
Sbjct: 121 GKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGG 180

Query: 184 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 243
           H  GGGYGNMMRKYGL+VD  IDA++VDVNGK+LDRK MGEDL+WAI GGGG S+GVV+A
Sbjct: 181 HISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLA 240

Query: 244 YKIRAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGK 303
           YKI  V VPE VTVF++ RTLE+N  T+I+  WQ+VA  +  +LFIR   DVVNGT   +
Sbjct: 241 YKINLVEVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQ 300

Query: 304 KTLRATFIALFLGDSERLLSVINKSFPKLGT-----------------------SVDALL 363
           KT+R TFIA+FLGD+  LLS++N+ FP+LG                        S + LL
Sbjct: 301 KTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLL 360

Query: 364 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 423
            ++ Q + +LKRKSDYV+  I + GL  IWKKMIELE P + FNPYGG M  I S  TPF
Sbjct: 361 LQRNQPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPF 420

Query: 424 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 483
           P+RAGNLWKIQY  NW +E     + +++LTRKL++FMTPFVSKNPR +F NYRD+D+GI
Sbjct: 421 PYRAGNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGI 480

Query: 484 N-HNGK-NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFP 506
           N HNGK +SY EG+ YG KYF GNF+RLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 481 NSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Lsi02G009670 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 543.1 bits (1398), Expect = 2.4e-154
Identity = 285/519 (54.91%), Postives = 358/519 (68.98%), Query Frame = 0

Query: 16  LVFFSSAT-----TAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRNLRFN 75
           L FFSSA      +  E+F QC   +   P   I   +F+  N S+SSVL  YIRN RFN
Sbjct: 20  LPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNGRFN 79

Query: 76  TSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDVIPFFVLDMF 135
           TS+TPKP +I+T   + HV  A+ C++  N  +KIRSGGHDYEG+SYISD  PFF+LDM 
Sbjct: 80  TSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDK-PFFILDMS 139

Query: 136 NLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGHFGGGGYGNM 195
           NLRD++VD+  ++AW+  GATLGEVYYRI EK+K  GFPAGVCPTVGVGGH  GGGYGNM
Sbjct: 140 NLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGYGNM 199

Query: 196 MRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRAVHVPE 255
           +RK+GLSVD +IDAKIVDVNG++LDRK+MGEDLFWAI GGGG+SFGVV+ YK++ V VPE
Sbjct: 200 LRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVPVPE 259

Query: 256 TVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKKTLRATFIAL 315
           TVTVF+VE+ ++   V ++V +WQ V    D++LF+R+    V  T K  KT+RAT +AL
Sbjct: 260 TVTVFRVEKYMDSGAV-DMVHKWQSVGPKTDRNLFLRMLIQPV--TRKKVKTVRATVVAL 319

Query: 316 FLGDSERLLSVINKSFPKLG------------------------TSVD--ALLSRKPQVL 375
           FLG +E +++++ K FP+L                         T +D    L R     
Sbjct: 320 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 379

Query: 376 THLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAGNL 435
              KRKSDYV + IP++G+  ++KKM EL K  L FNPYGG+MAE+  NATPFPHR+  L
Sbjct: 380 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 439

Query: 436 WKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGINHNGKNS 495
           +KIQY+  W E        F++    L+ FMT FVSKNPR A+LNYRD+DIG+N +G NS
Sbjct: 440 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNS 499

Query: 496 YYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIP 503
           Y EG VYG KYF  NFDRLVK+KT  DPDNFFRNEQSIP
Sbjct: 500 YEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of Lsi02G009670 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 531.6 bits (1368), Expect = 7.1e-151
Identity = 278/524 (53.05%), Postives = 358/524 (68.32%), Query Frame = 0

Query: 13  FSFLVF-------FSSATTAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIR 72
           F FL+F       FS + +   +F +C   +   P   I   +F+  N ++SSVL  YIR
Sbjct: 16  FFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIR 75

Query: 73  NLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDVIPFF 132
           N RFNTS+T KP +I+T   ESHV  A+ C++  N  +KIRSGGHDY+G+SYISD  PFF
Sbjct: 76  NARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK-PFF 135

Query: 133 VLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGHFGGG 192
           +LDM N+RD++VD+ + +AW+  GATLGEVYYRI EK++  GFPAGVCPTVGVGGH  GG
Sbjct: 136 ILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGG 195

Query: 193 GYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRA 252
           GYGNM+RK+GLSVD + DAKIVDVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK++ 
Sbjct: 196 GYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKL 255

Query: 253 VHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKKTLRA 312
           V VP  VTVF+VE+ ++   V ++V +WQ V    D +LF+R+    V  T K  KT+RA
Sbjct: 256 VPVPSVVTVFRVEQYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRA 315

Query: 313 TFIALFLGDSERLLSVINKSFPKLG------------------------TSVD--ALLSR 372
           + +ALFLG ++ ++++++K FP+LG                        T VD    L R
Sbjct: 316 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 375

Query: 373 KPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPH 432
                +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPH
Sbjct: 376 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 435

Query: 433 RAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGINH 492
           R   L+KIQY+ NW E        +++  + L+ FMT FVSKNPR+++ NYRD+DIG+N 
Sbjct: 436 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 495

Query: 493 NGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIP 503
           +G NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 496 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Lsi02G009670 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 531.6 bits (1368), Expect = 7.1e-151
Identity = 278/524 (53.05%), Postives = 358/524 (68.32%), Query Frame = 0

Query: 13  FSFLVF-------FSSATTAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIR 72
           F FL+F       FS + +   +F +C   +   P   I   +F+  N ++SSVL  YIR
Sbjct: 16  FFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIR 75

Query: 73  NLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDVIPFF 132
           N RFNTS+T KP +I+T   ESHV  A+ C++  N  +KIRSGGHDY+G+SYISD  PFF
Sbjct: 76  NARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK-PFF 135

Query: 133 VLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGHFGGG 192
           +LDM N+RD++VD+ + +AW+  GATLGEVYYRI EK++  GFPAGVCPTVGVGGH  GG
Sbjct: 136 ILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGG 195

Query: 193 GYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRA 252
           GYGNM+RK+GLSVD + DAKIVDVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK++ 
Sbjct: 196 GYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKL 255

Query: 253 VHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKKTLRA 312
           V VP  VTVF+VE+ ++   V ++V +WQ V    D +LF+R+    V  T K  KT+RA
Sbjct: 256 VPVPSVVTVFRVEQYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRA 315

Query: 313 TFIALFLGDSERLLSVINKSFPKLG------------------------TSVD--ALLSR 372
           + +ALFLG ++ ++++++K FP+LG                        T VD    L R
Sbjct: 316 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 375

Query: 373 KPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPH 432
                +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPH
Sbjct: 376 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 435

Query: 433 RAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGINH 492
           R   L+KIQY+ NW E        +++  + L+ FMT FVSKNPR+++ NYRD+DIG+N 
Sbjct: 436 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 495

Query: 493 NGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIP 503
           +G NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 496 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Lsi02G009670 vs. TAIR 10
Match: AT1G11770.1 (FAD-binding Berberine family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 3.0e-149
Identity = 279/529 (52.74%), Postives = 352/529 (66.54%), Query Frame = 0

Query: 8   ILLLSFSFLVFFSSATTAPE----NFFQCLLRHS-PPSYPIAPAIFTPNNASYSSVLETY 67
           IL L  SF+    +    PE    NF QC    +  P   +A  +     A+++ VL  Y
Sbjct: 7   ILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRAY 66

Query: 68  IRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYISDVIP 127
           IRN RFNT+ TPKP +++ A  ESHV+ A++C +  N+Q+K RSGGHDYEGVSYIS V P
Sbjct: 67  IRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISHV-P 126

Query: 128 FFVLDMFNLRDITVDVKTETAWVQTGATLGEVYYRIAEKNKSFGFPAGVCPTVGVGGHFG 187
           FFVLDM NLR+ITVD  TE+AWV  GATLGEVYYRI EK KS GFPAGVCPTVG GGH  
Sbjct: 127 FFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHIS 186

Query: 188 GGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKI 247
           GGGYGNM+RKYGLSVD + DAKIVDVNG++LDRK MGED+FWAI GGGG+SFGV++A+KI
Sbjct: 187 GGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFKI 246

Query: 248 RAVHVPETVTVFKVERTLEKNDVTEIVDEWQRVAHVIDKDLFIRVTFDVVNGTDKGKKTL 307
           + V VP TVTVF+VE+ L +N  TE+V +WQ VA   D  LF+R+    V  T    +T+
Sbjct: 247 KLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQTV 306

Query: 308 RATFIALFLGDSERLLSVINKSFPKLG--------------------------TSVDALL 367
           RA+ +ALFLGD   ++S++ K FP+LG                             + LL
Sbjct: 307 RASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEILL 366

Query: 368 SRKPQVLTHLKRKSDYVKTLIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 427
            R P + T  KRKSD+V+  I K+GL+ ++KKMIE+ K  L FNPYGG M+ + +  TPF
Sbjct: 367 DRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTPF 426

Query: 428 PHRAGNLWKIQYATNWDEEGTRQANHFIDLTRKLFKFMTPFVSKNPRTAFLNYRDLDIGI 487
           PHR   L+KIQ++ NW + GT     F+   +  + +M PFV+KNPR  ++NYRDLDIG+
Sbjct: 427 PHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIGV 486

Query: 488 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPDNFFRNEQSIPRFP 506
           N  G NSY    V+G  YF  NFDRLVK+KT VDP NFFR+EQSIP  P
Sbjct: 487 NTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530