Lsi02G009660 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G009660 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.7e-151
Identity = 257/506 (50.79%), Postives = 359/506 (70.95%), Query Frame = 0

Query: 66  ESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPFH 125
           E+F +C  S S  KH  PIS  +F + + S+SS+LQ +IRNLRF TT++PKP  ++   H
Sbjct: 26  ETFTQCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIAATH 85

Query: 126 EYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIETETA 185
           E HVQAAI C ++  LQ+++RSGGHDY+GLSYV+    PF VLD+ NL S+ +++ ++TA
Sbjct: 86  ESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVASKTA 145

Query: 186 SVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDA 245
            V+TGA LGE+YY I EKS    +PAG CPTVGVGGHISGGG+G + RKYGL  DN IDA
Sbjct: 146 WVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTIDA 205

Query: 246 KIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSLEQG 305
           +++D NG+I+DR  MGEDL+WAI GGGG S+GV+L++K+ LV VP  VTVF + R+LEQ 
Sbjct: 206 RMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQN 265

Query: 306 ATHLFQKWQTIAHKLHQDIFLHVTTKVTN--FPSKKTIKLSFTSLFLGPIERLIPLMNSH 365
           AT +  +WQ +A KL  ++F+     V N    S+KT++ +F ++FLG    L+ ++N  
Sbjct: 266 ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSILNRR 325

Query: 366 FPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKSDYVTSPIS 425
           FPELGL R DCTE SWIQSVLF+ ++   +   +L++R+ P    ++   KSDYV  PIS
Sbjct: 326 FPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRNQP---VNYLKRKSDYVREPIS 385

Query: 426 QNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLATWDNANET 485
           + GLE +W K++E E   +   PYGG+M +IS + TPFP+R G+++ IQY A W +   T
Sbjct: 386 RTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRDETLT 445

Query: 486 EKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRN--YGRNTSYEEAKVWGLKYFSDN 545
           ++++   R++Y +M P+VSK+PR ++ NYRD+DLG N   G+ +SY E K +G KYF+ N
Sbjct: 446 DRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGN 505

Query: 546 FERLVRVKTKVDPSNFFWNEQSIPLL 568
           FERLV++KT+VD  NFF NEQSIP+L
Sbjct: 506 FERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Lsi02G009660 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 1.8e-150
Identity = 264/507 (52.07%), Postives = 358/507 (70.61%), Query Frame = 0

Query: 65  RESFLECFNSTSYSKHSIPISEVVFTNE--SASFSSLLQLSIRNLRFLTTTSPKPLFLVT 124
           ++ F+ C      S  S P+    FT++  ++SF   L+ S +NLR+L  ++PKP F+  
Sbjct: 32  QQDFVMCL--VDNSDASFPMDSSFFTHDLNASSFKLALETSAQNLRYLMPSNPKPEFIFE 91

Query: 125 PFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIET 184
           P +E HVQAA++CA+K  L +R+RSGGHDYEGLSYVS     F+++DL  L  I ++IE+
Sbjct: 92  PLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLRQISVDIES 151

Query: 185 ETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNV 244
            +A V  GA++GE+YYRI EKS IHGFPAG C ++G+GGHI GG +G++ RK+GL ADNV
Sbjct: 152 NSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRKFGLGADNV 211

Query: 245 IDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSL 304
           +DA+I+D +G+I++R +MGED+FWAIRGGGG SFGVIL+WK+KLV VP IVTVF V R+L
Sbjct: 212 LDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVTVFTVTRTL 271

Query: 305 EQGATHLFQKWQTIAHKLHQDIFLHVTTKVTNFPSK---KTIKLSFTSLFLGPIERLIPL 364
           EQ  T L  KWQ +A KL +D+F+ V  + T+   K   +TI  S+   FLG   RL+ +
Sbjct: 272 EQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSYQGQFLGDANRLLQV 331

Query: 365 MNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKSDYVT 424
           M   FP+LGL + DC E SWI+SV++ A   +  P E L++  S  + +++F AKSDYV 
Sbjct: 332 MQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKS--LFKNYFKAKSDYVE 391

Query: 425 SPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLATWDN 484
            PI   GLEGLW KLLEE+    I  PYGG M++I E++TPFPHR G++F IQ+L  W +
Sbjct: 392 EPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFPHRSGTLFKIQWLTLWQD 451

Query: 485 ANETE-KHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYF 544
              +E KH+ W+RE+Y+YME YVSKSPR+AY+NYRDLDLG N G+ +   +A+ WG +YF
Sbjct: 452 GKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN-GKGS---DAREWGNRYF 511

Query: 545 SDNFERLVRVKTKVDPSNFFWNEQSIP 566
             NFERLV +K K DP NFF +EQSIP
Sbjct: 512 KGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Lsi02G009660 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 2.0e-149
Identity = 260/519 (50.10%), Postives = 356/519 (68.59%), Query Frame = 0

Query: 53  LCILVRKAKHRFRESFLECFNSTSYSKHSIPISEVVFT--NESASFSSLLQLSIRNLRFL 112
           L I V  +    ++ F++C      S  S PI+   F+    +  F   L+ + +NLR+L
Sbjct: 18  LLISVPLSSSTLQQDFVKCL--VDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYL 77

Query: 113 TTTSPKPLFLVTPFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDL 172
           T ++PKP+F+  P +E HVQAA+VCA+K  L +R+RSGGHDYEGLS+V+    PF+++DL
Sbjct: 78  TPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDL 137

Query: 173 INLSSIIINIETETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGT 232
             L  + +++++ +A    GAT+GE+YYRI EKS  HGFPAG C ++G+GGH+ GG +G+
Sbjct: 138 SKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGS 197

Query: 233 LFRKYGLAADNVIDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP 292
           + RK+GL ADNV+DA+I+D NG+I+DR +MGED+FWAIRGGGG SFGVIL+WK+KLV VP
Sbjct: 198 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 257

Query: 293 SIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHV---TTKVTNFPSKKTIKLSFTS 352
           + VTVF V ++LEQ  T +  KW+ IA KL  D+F+ V       T  P  +TI +S+ +
Sbjct: 258 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMSYQA 317

Query: 353 LFLGPIERLIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPIS 412
            FLG   RL+ +M   FPELGL + DCTEMSWI+SV++ A        E L+   S  + 
Sbjct: 318 QFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKS--LF 377

Query: 413 ESFFTAKSDYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGS 472
           ++ F AKSD+V  PI   GLEGLW + LEE+    I  PYGG MS+ISES+ PFPHR G+
Sbjct: 378 KNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHRNGT 437

Query: 473 IFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTS 532
           +F IQ+L+TW D     E+H+ WIRE+Y+YME YVSK+PR AY+NYRDLDLG N G    
Sbjct: 438 LFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGET-- 497

Query: 533 YEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 566
             +A+ WG KY+  NFERLV++K + DP NFF +EQS+P
Sbjct: 498 --DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Lsi02G009660 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.7e-148
Identity = 257/517 (49.71%), Postives = 357/517 (69.05%), Query Frame = 0

Query: 54  CILVRKAKHRFRESFLECFNSTSYSKHSIPISEVVFT--NESASFSSLLQLSIRNLRFLT 113
           CI    +    ++ F+ C    ++   S P+ + +FT     + F+ +L+ + +NL+FL 
Sbjct: 24  CITPTSSSASIQDQFINCVKRNTHV--SFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLA 83

Query: 114 TTSPKPLFLVTPFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLI 173
            + PKP F+  P H+  VQA+I+C++K G+  RVRSGGHD+E LSYVS    PFI+LDL 
Sbjct: 84  KSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLS 143

Query: 174 NLSSIIINIETETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTL 233
            L  I ++IE+ +A V+ GATLGELYYRIAEKS IHGFPAG C +VG+GG+++GGG+GTL
Sbjct: 144 KLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTL 203

Query: 234 FRKYGLAADNVIDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPS 293
            RKYGLA DNV+D K++D NG+++DR +MGEDLFWAIRGGGGASFG++L+WK+KLV VP 
Sbjct: 204 MRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPK 263

Query: 294 IVTVFNVQRSLEQGA-THLFQKWQTIAHKLHQDIFLHVTTKVTNFPSKKTIKLSFTSLFL 353
            VTVF V ++LEQ A      KWQ I+ K+ ++I + V  +       KT+ +++   FL
Sbjct: 264 TVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGNDGNKTVTMTYLGQFL 323

Query: 354 GPIERLIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESF 413
           G    L+ +M   FPELGL + DCTEMSWI++ LF     T +P+E+L++  S P+ + +
Sbjct: 324 GEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKS-PLGKDY 383

Query: 414 FTAKSDYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFG 473
           F A SD+V  PI   GL+G++ +L+E   + L  TPYGG MS+I ES  PFPHR G++F 
Sbjct: 384 FKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFK 443

Query: 474 IQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGR-NTSYE 533
           I Y A W +N   + + ++WI+E+Y YM PYVS +PR AY+NYRDLD G+N      ++ 
Sbjct: 444 ILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNAKVNFI 503

Query: 534 EAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 566
           EAK+WG KYF  NF+RLV++KTKVDP NFF +EQSIP
Sbjct: 504 EAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIP 537

BLAST of Lsi02G009660 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.9e-148
Identity = 252/504 (50.00%), Postives = 354/504 (70.24%), Query Frame = 0

Query: 65  RESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPF 124
           ++ F+ C    ++    +  +    T   + FS +L+ + +NLRFL  + PKP F+ +P 
Sbjct: 32  QDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPI 91

Query: 125 HEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIETET 184
           HE HVQA+I+C++K  + +RVRSGGHDYEGLSYVS    PFI++DL  +  + INI+  +
Sbjct: 92  HESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNS 151

Query: 185 ASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID 244
           A V++GAT+GELYYRIAEKS +HGFPAG C ++G+GGHI+GG +G++ RKYGL ADNV+D
Sbjct: 152 AWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLD 211

Query: 245 AKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSLEQ 304
           AKI+D NG+++DR +MGED FWAIRGG G SFG+IL+WK+KLV VP  VTVF V ++L+Q
Sbjct: 212 AKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQ 271

Query: 305 G-ATHLFQKWQTIAHKLHQDIFLHVTTKVTNFPSKKTIKLSFTSLFLGPIERLIPLMNSH 364
                +  KWQ +A KL +++F+ V   V      KT+  S+ +LFLG    L+ +M   
Sbjct: 272 DVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKS 331

Query: 365 FPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKSDYVTSPIS 424
           FPELGL   DC EMSW++S+ + +   T TP  VL++  S P  +  F AKSD+V +PI 
Sbjct: 332 FPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKS-PFPKVSFKAKSDFVKTPIP 391

Query: 425 QNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLATW-DNANE 484
           ++GL+G++ KLL+E+   +I  PYGG M++I ES+ PFPHRKG +F +QY+ +W D+   
Sbjct: 392 ESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKR 451

Query: 485 TEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRN-YGRNTSYEEAKVWGLKYFSDN 544
             +H++WIR++Y+YM PYVS +PR AY+NYRDLDLGRN     T  ++A+VWG  YF +N
Sbjct: 452 PSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNN 511

Query: 545 FERLVRVKTKVDPSNFFWNEQSIP 566
           F RL+ +K KVDP NFF +EQSIP
Sbjct: 512 FNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of Lsi02G009660 vs. ExPASy TrEMBL
Match: A0A5A7TYX1 (Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold437G00060 PE=3 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.7e-274
Identity = 478/542 (88.19%), Postives = 503/542 (92.80%), Query Frame = 0

Query: 51  CDLCILVRKAKHRFRESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFL 110
           C   I +  +    +++FLECFNSTSYSKHSIP+SEVVFTNESASFSSL +LSIRNLRFL
Sbjct: 16  CVSLIWLCSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLFRLSIRNLRFL 75

Query: 111 TTTSPKPLFLVTPFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDL 170
           TTT PKPLFLVTPFHE HVQAAIVCAR+KGLQVRVRSGGHDYEGLSYVSSQ APFIV+DL
Sbjct: 76  TTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYVSSQ-APFIVIDL 135

Query: 171 INLSSIIINIETETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGT 230
           INL SI I+IE ETASV TGA LGELYYRIA+KS IHGFPAGSCPTVGVGGHISGGGFGT
Sbjct: 136 INLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGT 195

Query: 231 LFRKYGLAADNVIDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP 290
           LFRKYGLAADNVIDAKIIDFNGRIMDR+SMGEDLFWAIRGGGGASFGVILSWKLKLVSVP
Sbjct: 196 LFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP 255

Query: 291 SIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVTTK-VTNFPSKKTIKLSFTSLF 350
           SIVTVFNVQR+LEQGATHLFQKWQ I+HKL QDIFLHVTTK VT+FPSKKTI+LSFTSLF
Sbjct: 256 SIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFPSKKTIRLSFTSLF 315

Query: 351 LGPIERLIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISES 410
           LGPIERLIP+M + F ELGLKR DC EMSWIQSVLFFAD S +TPLEVLMERSSP IS++
Sbjct: 316 LGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEVLMERSSPQISDA 375

Query: 411 FFTAKSDYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIF 470
           FFTAKSDYV SPISQ GLEGLW KLLEEEKSELILTPYGGKMSQISES+TPFPHR+G IF
Sbjct: 376 FFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISESQTPFPHREGRIF 435

Query: 471 GIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEE 530
           GIQYLATWDNANETEKHLSWIREVY YM+PYVSKSPRAAYLNYRDLDLGRNYGRNTSYEE
Sbjct: 436 GIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEE 495

Query: 531 AKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIEKVYSVLDFEIVP 590
           AKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRI KV+S LDFEIV 
Sbjct: 496 AKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRITKVHSGLDFEIVQ 555

Query: 591 ER 592
           ER
Sbjct: 556 ER 556

BLAST of Lsi02G009660 vs. ExPASy TrEMBL
Match: A0A1S3BE11 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103488588 PE=3 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.7e-274
Identity = 478/542 (88.19%), Postives = 503/542 (92.80%), Query Frame = 0

Query: 51  CDLCILVRKAKHRFRESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFL 110
           C   I +  +    +++FLECFNSTSYSKHSIP+SEVVFTNESASFSSL +LSIRNLRFL
Sbjct: 16  CVSLIWLCSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLFRLSIRNLRFL 75

Query: 111 TTTSPKPLFLVTPFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDL 170
           TTT PKPLFLVTPFHE HVQAAIVCAR+KGLQVRVRSGGHDYEGLSYVSSQ APFIV+DL
Sbjct: 76  TTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYVSSQ-APFIVIDL 135

Query: 171 INLSSIIINIETETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGT 230
           INL SI I+IE ETASV TGA LGELYYRIA+KS IHGFPAGSCPTVGVGGHISGGGFGT
Sbjct: 136 INLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGT 195

Query: 231 LFRKYGLAADNVIDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP 290
           LFRKYGLAADNVIDAKIIDFNGRIMDR+SMGEDLFWAIRGGGGASFGVILSWKLKLVSVP
Sbjct: 196 LFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP 255

Query: 291 SIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVTTK-VTNFPSKKTIKLSFTSLF 350
           SIVTVFNVQR+LEQGATHLFQKWQ I+HKL QDIFLHVTTK VT+FPSKKTI+LSFTSLF
Sbjct: 256 SIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFPSKKTIRLSFTSLF 315

Query: 351 LGPIERLIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISES 410
           LGPIERLIP+M + F ELGLKR DC EMSWIQSVLFFAD S +TPLEVLMERSSP IS++
Sbjct: 316 LGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEVLMERSSPQISDA 375

Query: 411 FFTAKSDYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIF 470
           FFTAKSDYV SPISQ GLEGLW KLLEEEKSELILTPYGGKMSQISES+TPFPHR+G IF
Sbjct: 376 FFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISESQTPFPHREGRIF 435

Query: 471 GIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEE 530
           GIQYLATWDNANETEKHLSWIREVY YM+PYVSKSPRAAYLNYRDLDLGRNYGRNTSYEE
Sbjct: 436 GIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEE 495

Query: 531 AKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIEKVYSVLDFEIVP 590
           AKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRI KV+S LDFEIV 
Sbjct: 496 AKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRITKVHSGLDFEIVQ 555

Query: 591 ER 592
           ER
Sbjct: 556 ER 556

BLAST of Lsi02G009660 vs. ExPASy TrEMBL
Match: A0A6J1CHF0 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE=3 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.9e-257
Identity = 449/537 (83.61%), Postives = 489/537 (91.06%), Query Frame = 0

Query: 65  RESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPF 124
           +ESF+ECFNST+YSKHS+PISEVVFTNESASFSSLL+LSIRNLRFLT +SPKP FLVTPF
Sbjct: 26  QESFMECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPF 85

Query: 125 HEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIETET 184
              HVQAA+VCA+KK LQVRVRSGGHDYEGLSYVSSQ  PFIV+DLINLSSI I+IETET
Sbjct: 86  RASHVQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQ-TPFIVIDLINLSSIHIDIETET 145

Query: 185 ASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID 244
           ASVETGATLGE+YYRIAEKSPIHGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVID
Sbjct: 146 ASVETGATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVID 205

Query: 245 AKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSLEQ 304
           AKI+DFNGRI DRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP  VTVFNV R+L+Q
Sbjct: 206 AKIVDFNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQ 265

Query: 305 GATHLFQKWQTIAHKLHQDIFLHVTTKVTNFPSK---------KTIKLSFTSLFLGPIER 364
           GATHLFQKWQ IAHK+ QD+FLHVTTKV +  +K         KTIKLSFTSLFLGPIER
Sbjct: 266 GATHLFQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIER 325

Query: 365 LIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKS 424
           LIPLMN+HF ELGLKR DCTEMSWIQSVLFFADLSTETP E+LMER SPPIS++FFTAKS
Sbjct: 326 LIPLMNTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMER-SPPISDTFFTAKS 385

Query: 425 DYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLA 484
           DYVTSPISQ GLE LWSKLLEEEKSELILTPYGGKM QISES+TPFPHR+G+I+GIQYLA
Sbjct: 386 DYVTSPISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLA 445

Query: 485 TWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGL 544
            W+NANETEKHLSW+REVY+YM+P+VSK PR AYLNYRDLDLGRNY RNTSY+EAKVWGL
Sbjct: 446 AWENANETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGL 505

Query: 545 KYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDD-TRIEKVYSVLDFEIVPER 592
           KYF  NFERLVRVKTKVDP NFFWNEQSIPL +H+E++ TRI +V S LD +I+ ER
Sbjct: 506 KYFKGNFERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRILQVNSYLDSKILQER 560

BLAST of Lsi02G009660 vs. ExPASy TrEMBL
Match: A0A6J1JUS4 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111489931 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.5e-248
Identity = 428/531 (80.60%), Postives = 476/531 (89.64%), Query Frame = 0

Query: 65  RESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPF 124
           +E+FLECFNST+YSKHS+P+SEVVF+NESASFSSLL+ SIRNLRFL+T+SPKPLFLVTPF
Sbjct: 30  QENFLECFNSTTYSKHSLPVSEVVFSNESASFSSLLKHSIRNLRFLSTSSPKPLFLVTPF 89

Query: 125 HEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIETET 184
           HE HVQAAIVCA++  LQVRV SGGHDYEGLSY+SS  APFIV+DLINL SI INI+TET
Sbjct: 90  HESHVQAAIVCAKENDLQVRVLSGGHDYEGLSYLSSSQAPFIVIDLINLRSIDINIKTET 149

Query: 185 ASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID 244
           A VETGAT+GELYYRIA+KSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID
Sbjct: 150 ALVETGATIGELYYRIAKKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID 209

Query: 245 AKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSLEQ 304
           AKI+DFNGRIMDRN+MGEDLFWAIRGGGGASFGVILSWKLKLVS+PSIVT FNVQR+L Q
Sbjct: 210 AKIVDFNGRIMDRNTMGEDLFWAIRGGGGASFGVILSWKLKLVSLPSIVTTFNVQRTLNQ 269

Query: 305 GATHLFQKWQTIAHKLHQDIFLHVTTKVTN----FPSKKTIKLSFTSLFLGPIERLIPLM 364
           GATHLFQKWQ IAHKL QD+FLHVTTKV +      + KT+ LSFTSLFLG I+ LIPLM
Sbjct: 270 GATHLFQKWQQIAHKLDQDLFLHVTTKVIDQKMTKTTSKTLSLSFTSLFLGSIDSLIPLM 329

Query: 365 NSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKSDYVTS 424
           ++HFPELGLKR  CTEM+WIQS+LFFAD ST++PLE+LM+R   P + S F AKSDYVTS
Sbjct: 330 DTHFPELGLKRYQCTEMNWIQSILFFADFSTKSPLEILMQRL--PSTRSSFIAKSDYVTS 389

Query: 425 PISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLATWDNA 484
           PISQ+GLEGLW+KLLE E SELI TPYGGKMSQISE +TPFPHRKGSIFG+QYLATWD++
Sbjct: 390 PISQSGLEGLWAKLLENENSELIFTPYGGKMSQISELETPFPHRKGSIFGVQYLATWDDS 449

Query: 485 NETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFSD 544
           NE E+HL WIREVY+YMEPYVS +PR  YLNYRDLDLGRNYGRNTSYEEAKVWGLKYF  
Sbjct: 450 NENERHLRWIREVYSYMEPYVSMAPRGTYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFKG 509

Query: 545 NFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIEKVYSVLDFEIVPER 592
           NFERLV+VKTK DP NFFWNEQSIPLLY+ ED+ RI +VYS L+ E V ER
Sbjct: 510 NFERLVKVKTKFDPLNFFWNEQSIPLLYNCEDEARICEVYSDLNSENVQER 558

BLAST of Lsi02G009660 vs. ExPASy TrEMBL
Match: A0A6J1GQY4 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111456716 PE=3 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 4.8e-247
Identity = 427/531 (80.41%), Postives = 473/531 (89.08%), Query Frame = 0

Query: 65  RESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPF 124
           +E+F+ECFNST+YSKHS+P+SEVVF+NESASFSSLL+ SIRNLRFL+T+SPKPLFLVTPF
Sbjct: 28  QENFIECFNSTTYSKHSLPVSEVVFSNESASFSSLLKHSIRNLRFLSTSSPKPLFLVTPF 87

Query: 125 HEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIETET 184
           HE HVQAAIVCA++  LQVRV SGGHDYEGLSY+SS  APFIV+DLINL SI INIETET
Sbjct: 88  HESHVQAAIVCAKENDLQVRVLSGGHDYEGLSYLSSSQAPFIVIDLINLRSIDINIETET 147

Query: 185 ASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID 244
           ASVETGATLGELYYRIA+KSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID
Sbjct: 148 ASVETGATLGELYYRIAKKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID 207

Query: 245 AKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSLEQ 304
           AKI+DFNGRIMDRN+M EDLFWAIRGGGGASFGVILSWKLKLVS+PSIVT FNVQR+L Q
Sbjct: 208 AKIVDFNGRIMDRNTMEEDLFWAIRGGGGASFGVILSWKLKLVSLPSIVTTFNVQRTLNQ 267

Query: 305 GATHLFQKWQTIAHKLHQDIFLHVTTKVTN----FPSKKTIKLSFTSLFLGPIERLIPLM 364
           G THLF KWQ IAHKL+QD+FLHVTTKV +      + KT+ LSFTSLFLG I+ LIPLM
Sbjct: 268 GGTHLFLKWQEIAHKLNQDLFLHVTTKVIDQKMTKTTSKTLSLSFTSLFLGSIDTLIPLM 327

Query: 365 NSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKSDYVTS 424
           ++HFPELGLKR  CTEM+WIQSVLFFAD ST++PLE+LM+R   P   S F AKSDYVTS
Sbjct: 328 DTHFPELGLKRYQCTEMNWIQSVLFFADFSTKSPLEILMQRL--PSIRSSFIAKSDYVTS 387

Query: 425 PISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLATWDNA 484
           PISQ+GLEGLW+KLLE + SELI TPYGGKMSQISE +TPFPHRKGSIFG+QYLATWD++
Sbjct: 388 PISQSGLEGLWAKLLENDNSELIFTPYGGKMSQISELETPFPHRKGSIFGVQYLATWDDS 447

Query: 485 NETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFSD 544
           NE E+HL WIREVY+YMEPYVS +PR  YLNYRDLDLGRNYGRNTSYEEAKVWGLKYF  
Sbjct: 448 NENERHLRWIREVYSYMEPYVSMAPRGTYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFKG 507

Query: 545 NFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIEKVYSVLDFEIVPER 592
           NFERLV+VKTK DP NFFWNEQSIPLLY+ ED+ RI +VYS LD   V ER
Sbjct: 508 NFERLVKVKTKFDPLNFFWNEQSIPLLYNCEDEARICEVYSDLDSANVQER 556

BLAST of Lsi02G009660 vs. NCBI nr
Match: XP_038895849.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 984.6 bits (2544), Expect = 3.8e-283
Identity = 491/528 (92.99%), Postives = 508/528 (96.21%), Query Frame = 0

Query: 65  RESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPF 124
           +E FLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTT PKPLFLVTPF
Sbjct: 28  QERFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTMPKPLFLVTPF 87

Query: 125 HEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIETET 184
           HE HVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQ APFIV+DLINL SI I+IETET
Sbjct: 88  HESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQ-APFIVIDLINLHSITIDIETET 147

Query: 185 ASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID 244
            SVETGATLGELYY+IAEKSPIHGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVID
Sbjct: 148 TSVETGATLGELYYKIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVID 207

Query: 245 AKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSLEQ 304
           AKI+DFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQR+LEQ
Sbjct: 208 AKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQ 267

Query: 305 GATHLFQKWQTIAHKLHQDIFLHVTTKV-TNFPSKKTIKLSFTSLFLGPIERLIPLMNSH 364
           GATHLFQKWQTIAHKL QDIFLHVTTKV TNF SKKTI+LSFTSLFLGPIERLIP+M +H
Sbjct: 268 GATHLFQKWQTIAHKLDQDIFLHVTTKVITNFSSKKTIRLSFTSLFLGPIERLIPIMKTH 327

Query: 365 FPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKSDYVTSPIS 424
           F ELGLKR DCTEMSWIQSVLFFAD S ETPLEVLMERSSPPIS++FFTAKSDYVTSPIS
Sbjct: 328 FSELGLKRNDCTEMSWIQSVLFFADFSIETPLEVLMERSSPPISDAFFTAKSDYVTSPIS 387

Query: 425 QNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLATWDNANET 484
           QNGLEGLW+KLLEEEKSELILTPYGGKMSQISES+TPFPHR+GSIFGIQYLATWDNANET
Sbjct: 388 QNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQTPFPHRRGSIFGIQYLATWDNANET 447

Query: 485 EKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFSDNFE 544
           EKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNY +NTSYEEAKVWGLKYFSDNFE
Sbjct: 448 EKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYRKNTSYEEAKVWGLKYFSDNFE 507

Query: 545 RLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIEKVYSVLDFEIVPER 592
           RLVRVKTKVDP NFFWNEQSIPLLYHYEDDTRI KV+S LDFEIV ER
Sbjct: 508 RLVRVKTKVDPLNFFWNEQSIPLLYHYEDDTRISKVHSGLDFEIVQER 554

BLAST of Lsi02G009660 vs. NCBI nr
Match: XP_008445632.1 (PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo] >KAA0047127.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa] >TYK14944.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa])

HSP 1 Score: 954.1 bits (2465), Expect = 5.5e-274
Identity = 478/542 (88.19%), Postives = 503/542 (92.80%), Query Frame = 0

Query: 51  CDLCILVRKAKHRFRESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFL 110
           C   I +  +    +++FLECFNSTSYSKHSIP+SEVVFTNESASFSSL +LSIRNLRFL
Sbjct: 16  CVSLIWLCSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFSSLFRLSIRNLRFL 75

Query: 111 TTTSPKPLFLVTPFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDL 170
           TTT PKPLFLVTPFHE HVQAAIVCAR+KGLQVRVRSGGHDYEGLSYVSSQ APFIV+DL
Sbjct: 76  TTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSYVSSQ-APFIVIDL 135

Query: 171 INLSSIIINIETETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGT 230
           INL SI I+IE ETASV TGA LGELYYRIA+KS IHGFPAGSCPTVGVGGHISGGGFGT
Sbjct: 136 INLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGT 195

Query: 231 LFRKYGLAADNVIDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP 290
           LFRKYGLAADNVIDAKIIDFNGRIMDR+SMGEDLFWAIRGGGGASFGVILSWKLKLVSVP
Sbjct: 196 LFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP 255

Query: 291 SIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVTTK-VTNFPSKKTIKLSFTSLF 350
           SIVTVFNVQR+LEQGATHLFQKWQ I+HKL QDIFLHVTTK VT+FPSKKTI+LSFTSLF
Sbjct: 256 SIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFPSKKTIRLSFTSLF 315

Query: 351 LGPIERLIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISES 410
           LGPIERLIP+M + F ELGLKR DC EMSWIQSVLFFAD S +TPLEVLMERSSP IS++
Sbjct: 316 LGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEVLMERSSPQISDA 375

Query: 411 FFTAKSDYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIF 470
           FFTAKSDYV SPISQ GLEGLW KLLEEEKSELILTPYGGKMSQISES+TPFPHR+G IF
Sbjct: 376 FFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISESQTPFPHREGRIF 435

Query: 471 GIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEE 530
           GIQYLATWDNANETEKHLSWIREVY YM+PYVSKSPRAAYLNYRDLDLGRNYGRNTSYEE
Sbjct: 436 GIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEE 495

Query: 531 AKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIEKVYSVLDFEIVP 590
           AKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRI KV+S LDFEIV 
Sbjct: 496 AKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRITKVHSGLDFEIVQ 555

Query: 591 ER 592
           ER
Sbjct: 556 ER 556

BLAST of Lsi02G009660 vs. NCBI nr
Match: KAE8653305.1 (hypothetical protein Csa_023319 [Cucumis sativus])

HSP 1 Score: 943.0 bits (2436), Expect = 1.3e-270
Identity = 470/544 (86.40%), Postives = 504/544 (92.65%), Query Frame = 0

Query: 53  LCI----LVRKAKHRFRESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLR 112
           LC+    L   +    +++FLECFNSTSYSKHSIPISEVVFTNESASFSSLL+LSIRNLR
Sbjct: 15  LCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNLR 74

Query: 113 FLTTTSPKPLFLVTPFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVL 172
           FLTTT PKPL LVTPFHE HVQAAI+CAR+KGLQVRVRSGGHDYEGLSY+SSQ APFIV+
Sbjct: 75  FLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQ-APFIVI 134

Query: 173 DLINLSSIIINIETETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGF 232
           DLINL SI I+I+TETASVETGA+LGELYYRIA+KS IHGFPAGSCPTVGVGGHISGGGF
Sbjct: 135 DLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGF 194

Query: 233 GTLFRKYGLAADNVIDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVS 292
           GTLFRKYGLAADNVIDAKIIDFNGRIMDR+SMGEDLFWAIRGGGGASFGVILSWKLKLVS
Sbjct: 195 GTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVS 254

Query: 293 VPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVTTK-VTNFPSKKTIKLSFTS 352
           VPSIVTVFNVQR+LE+GATHLFQKWQ I+HKL QDIFLHVTTK VTNFPSKKTI+LSFTS
Sbjct: 255 VPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFPSKKTIRLSFTS 314

Query: 353 LFLGPIERLIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPIS 412
           LFLGPIERLIP+M + F ELGLKR DC EMSWIQSVLFFAD S + PLEVLM+RSSP IS
Sbjct: 315 LFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDRSSPQIS 374

Query: 413 ESFFTAKSDYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGS 472
           ++FFTAKSDYVTSPIS+NGLEGLWSKLLEE+KSELI TPYGGKMSQISES+ PFPHR+G 
Sbjct: 375 DAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPFPHREGR 434

Query: 473 IFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSY 532
           IFGIQYLATWDNANE EKHLSWIREVY YME YVSKSPRAAYLNYRDLDLG NYGRNTSY
Sbjct: 435 IFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNYGRNTSY 494

Query: 533 EEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIEKVYSVLDFEI 592
           EEAKVWGLKYFSDNF+RLVRVKTKVDPSNFFWNEQSIPLLYHYEDDT++ KV+S LDFEI
Sbjct: 495 EEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKVTKVHSGLDFEI 554

BLAST of Lsi02G009660 vs. NCBI nr
Match: XP_004135724.1 (berberine bridge enzyme-like 13 [Cucumis sativus])

HSP 1 Score: 943.0 bits (2436), Expect = 1.3e-270
Identity = 470/544 (86.40%), Postives = 504/544 (92.65%), Query Frame = 0

Query: 53  LCI----LVRKAKHRFRESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLR 112
           LC+    L   +    +++FLECFNSTSYSKHSIPISEVVFTNESASFSSLL+LSIRNLR
Sbjct: 13  LCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNLR 72

Query: 113 FLTTTSPKPLFLVTPFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVL 172
           FLTTT PKPL LVTPFHE HVQAAI+CAR+KGLQVRVRSGGHDYEGLSY+SSQ APFIV+
Sbjct: 73  FLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQ-APFIVI 132

Query: 173 DLINLSSIIINIETETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGF 232
           DLINL SI I+I+TETASVETGA+LGELYYRIA+KS IHGFPAGSCPTVGVGGHISGGGF
Sbjct: 133 DLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGF 192

Query: 233 GTLFRKYGLAADNVIDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVS 292
           GTLFRKYGLAADNVIDAKIIDFNGRIMDR+SMGEDLFWAIRGGGGASFGVILSWKLKLVS
Sbjct: 193 GTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVS 252

Query: 293 VPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVTTK-VTNFPSKKTIKLSFTS 352
           VPSIVTVFNVQR+LE+GATHLFQKWQ I+HKL QDIFLHVTTK VTNFPSKKTI+LSFTS
Sbjct: 253 VPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFPSKKTIRLSFTS 312

Query: 353 LFLGPIERLIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPIS 412
           LFLGPIERLIP+M + F ELGLKR DC EMSWIQSVLFFAD S + PLEVLM+RSSP IS
Sbjct: 313 LFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDRSSPQIS 372

Query: 413 ESFFTAKSDYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGS 472
           ++FFTAKSDYVTSPIS+NGLEGLWSKLLEE+KSELI TPYGGKMSQISES+ PFPHR+G 
Sbjct: 373 DAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPFPHREGR 432

Query: 473 IFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSY 532
           IFGIQYLATWDNANE EKHLSWIREVY YME YVSKSPRAAYLNYRDLDLG NYGRNTSY
Sbjct: 433 IFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNYGRNTSY 492

Query: 533 EEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIEKVYSVLDFEI 592
           EEAKVWGLKYFSDNF+RLVRVKTKVDPSNFFWNEQSIPLLYHYEDDT++ KV+S LDFEI
Sbjct: 493 EEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKVTKVHSGLDFEI 552

BLAST of Lsi02G009660 vs. NCBI nr
Match: XP_022140178.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 897.1 bits (2317), Expect = 8.0e-257
Identity = 449/537 (83.61%), Postives = 489/537 (91.06%), Query Frame = 0

Query: 65  RESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPF 124
           +ESF+ECFNST+YSKHS+PISEVVFTNESASFSSLL+LSIRNLRFLT +SPKP FLVTPF
Sbjct: 26  QESFMECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPF 85

Query: 125 HEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIETET 184
              HVQAA+VCA+KK LQVRVRSGGHDYEGLSYVSSQ  PFIV+DLINLSSI I+IETET
Sbjct: 86  RASHVQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQ-TPFIVIDLINLSSIHIDIETET 145

Query: 185 ASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID 244
           ASVETGATLGE+YYRIAEKSPIHGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVID
Sbjct: 146 ASVETGATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVID 205

Query: 245 AKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSLEQ 304
           AKI+DFNGRI DRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP  VTVFNV R+L+Q
Sbjct: 206 AKIVDFNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQ 265

Query: 305 GATHLFQKWQTIAHKLHQDIFLHVTTKVTNFPSK---------KTIKLSFTSLFLGPIER 364
           GATHLFQKWQ IAHK+ QD+FLHVTTKV +  +K         KTIKLSFTSLFLGPIER
Sbjct: 266 GATHLFQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIER 325

Query: 365 LIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKS 424
           LIPLMN+HF ELGLKR DCTEMSWIQSVLFFADLSTETP E+LMER SPPIS++FFTAKS
Sbjct: 326 LIPLMNTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMER-SPPISDTFFTAKS 385

Query: 425 DYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLA 484
           DYVTSPISQ GLE LWSKLLEEEKSELILTPYGGKM QISES+TPFPHR+G+I+GIQYLA
Sbjct: 386 DYVTSPISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLA 445

Query: 485 TWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGL 544
            W+NANETEKHLSW+REVY+YM+P+VSK PR AYLNYRDLDLGRNY RNTSY+EAKVWGL
Sbjct: 446 AWENANETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGL 505

Query: 545 KYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDD-TRIEKVYSVLDFEIVPER 592
           KYF  NFERLVRVKTKVDP NFFWNEQSIPL +H+E++ TRI +V S LD +I+ ER
Sbjct: 506 KYFKGNFERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRILQVNSYLDSKILQER 560

BLAST of Lsi02G009660 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 536.2 bits (1380), Expect = 3.4e-152
Identity = 257/506 (50.79%), Postives = 359/506 (70.95%), Query Frame = 0

Query: 66  ESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPFH 125
           E+F +C  S S  KH  PIS  +F + + S+SS+LQ +IRNLRF TT++PKP  ++   H
Sbjct: 26  ETFTQCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIAATH 85

Query: 126 EYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIETETA 185
           E HVQAAI C ++  LQ+++RSGGHDY+GLSYV+    PF VLD+ NL S+ +++ ++TA
Sbjct: 86  ESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVASKTA 145

Query: 186 SVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDA 245
            V+TGA LGE+YY I EKS    +PAG CPTVGVGGHISGGG+G + RKYGL  DN IDA
Sbjct: 146 WVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTIDA 205

Query: 246 KIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSLEQG 305
           +++D NG+I+DR  MGEDL+WAI GGGG S+GV+L++K+ LV VP  VTVF + R+LEQ 
Sbjct: 206 RMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQN 265

Query: 306 ATHLFQKWQTIAHKLHQDIFLHVTTKVTN--FPSKKTIKLSFTSLFLGPIERLIPLMNSH 365
           AT +  +WQ +A KL  ++F+     V N    S+KT++ +F ++FLG    L+ ++N  
Sbjct: 266 ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSILNRR 325

Query: 366 FPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKSDYVTSPIS 425
           FPELGL R DCTE SWIQSVLF+ ++   +   +L++R+ P    ++   KSDYV  PIS
Sbjct: 326 FPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRNQP---VNYLKRKSDYVREPIS 385

Query: 426 QNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLATWDNANET 485
           + GLE +W K++E E   +   PYGG+M +IS + TPFP+R G+++ IQY A W +   T
Sbjct: 386 RTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRDETLT 445

Query: 486 EKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRN--YGRNTSYEEAKVWGLKYFSDN 545
           ++++   R++Y +M P+VSK+PR ++ NYRD+DLG N   G+ +SY E K +G KYF+ N
Sbjct: 446 DRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGN 505

Query: 546 FERLVRVKTKVDPSNFFWNEQSIPLL 568
           FERLV++KT+VD  NFF NEQSIP+L
Sbjct: 506 FERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Lsi02G009660 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 534.3 bits (1375), Expect = 1.3e-151
Identity = 264/507 (52.07%), Postives = 358/507 (70.61%), Query Frame = 0

Query: 65  RESFLECFNSTSYSKHSIPISEVVFTNE--SASFSSLLQLSIRNLRFLTTTSPKPLFLVT 124
           ++ F+ C      S  S P+    FT++  ++SF   L+ S +NLR+L  ++PKP F+  
Sbjct: 32  QQDFVMCL--VDNSDASFPMDSSFFTHDLNASSFKLALETSAQNLRYLMPSNPKPEFIFE 91

Query: 125 PFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIET 184
           P +E HVQAA++CA+K  L +R+RSGGHDYEGLSYVS     F+++DL  L  I ++IE+
Sbjct: 92  PLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLRQISVDIES 151

Query: 185 ETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNV 244
            +A V  GA++GE+YYRI EKS IHGFPAG C ++G+GGHI GG +G++ RK+GL ADNV
Sbjct: 152 NSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRKFGLGADNV 211

Query: 245 IDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSL 304
           +DA+I+D +G+I++R +MGED+FWAIRGGGG SFGVIL+WK+KLV VP IVTVF V R+L
Sbjct: 212 LDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVTVFTVTRTL 271

Query: 305 EQGATHLFQKWQTIAHKLHQDIFLHVTTKVTNFPSK---KTIKLSFTSLFLGPIERLIPL 364
           EQ  T L  KWQ +A KL +D+F+ V  + T+   K   +TI  S+   FLG   RL+ +
Sbjct: 272 EQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSYQGQFLGDANRLLQV 331

Query: 365 MNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKSDYVT 424
           M   FP+LGL + DC E SWI+SV++ A   +  P E L++  S  + +++F AKSDYV 
Sbjct: 332 MQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKS--LFKNYFKAKSDYVE 391

Query: 425 SPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLATWDN 484
            PI   GLEGLW KLLEE+    I  PYGG M++I E++TPFPHR G++F IQ+L  W +
Sbjct: 392 EPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFPHRSGTLFKIQWLTLWQD 451

Query: 485 ANETE-KHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYF 544
              +E KH+ W+RE+Y+YME YVSKSPR+AY+NYRDLDLG N G+ +   +A+ WG +YF
Sbjct: 452 GKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN-GKGS---DAREWGNRYF 511

Query: 545 SDNFERLVRVKTKVDPSNFFWNEQSIP 566
             NFERLV +K K DP NFF +EQSIP
Sbjct: 512 KGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Lsi02G009660 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 530.8 bits (1366), Expect = 1.4e-150
Identity = 260/519 (50.10%), Postives = 356/519 (68.59%), Query Frame = 0

Query: 53  LCILVRKAKHRFRESFLECFNSTSYSKHSIPISEVVFT--NESASFSSLLQLSIRNLRFL 112
           L I V  +    ++ F++C      S  S PI+   F+    +  F   L+ + +NLR+L
Sbjct: 18  LLISVPLSSSTLQQDFVKCL--VDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYL 77

Query: 113 TTTSPKPLFLVTPFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDL 172
           T ++PKP+F+  P +E HVQAA+VCA+K  L +R+RSGGHDYEGLS+V+    PF+++DL
Sbjct: 78  TPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDL 137

Query: 173 INLSSIIINIETETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGT 232
             L  + +++++ +A    GAT+GE+YYRI EKS  HGFPAG C ++G+GGH+ GG +G+
Sbjct: 138 SKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGS 197

Query: 233 LFRKYGLAADNVIDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP 292
           + RK+GL ADNV+DA+I+D NG+I+DR +MGED+FWAIRGGGG SFGVIL+WK+KLV VP
Sbjct: 198 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 257

Query: 293 SIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHV---TTKVTNFPSKKTIKLSFTS 352
           + VTVF V ++LEQ  T +  KW+ IA KL  D+F+ V       T  P  +TI +S+ +
Sbjct: 258 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMSYQA 317

Query: 353 LFLGPIERLIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPIS 412
            FLG   RL+ +M   FPELGL + DCTEMSWI+SV++ A        E L+   S  + 
Sbjct: 318 QFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKS--LF 377

Query: 413 ESFFTAKSDYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGS 472
           ++ F AKSD+V  PI   GLEGLW + LEE+    I  PYGG MS+ISES+ PFPHR G+
Sbjct: 378 KNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHRNGT 437

Query: 473 IFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTS 532
           +F IQ+L+TW D     E+H+ WIRE+Y+YME YVSK+PR AY+NYRDLDLG N G    
Sbjct: 438 LFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGET-- 497

Query: 533 YEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 566
             +A+ WG KY+  NFERLV++K + DP NFF +EQS+P
Sbjct: 498 --DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Lsi02G009660 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 527.7 bits (1358), Expect = 1.2e-149
Identity = 257/517 (49.71%), Postives = 357/517 (69.05%), Query Frame = 0

Query: 54  CILVRKAKHRFRESFLECFNSTSYSKHSIPISEVVFT--NESASFSSLLQLSIRNLRFLT 113
           CI    +    ++ F+ C    ++   S P+ + +FT     + F+ +L+ + +NL+FL 
Sbjct: 24  CITPTSSSASIQDQFINCVKRNTHV--SFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLA 83

Query: 114 TTSPKPLFLVTPFHEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLI 173
            + PKP F+  P H+  VQA+I+C++K G+  RVRSGGHD+E LSYVS    PFI+LDL 
Sbjct: 84  KSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLS 143

Query: 174 NLSSIIINIETETASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTL 233
            L  I ++IE+ +A V+ GATLGELYYRIAEKS IHGFPAG C +VG+GG+++GGG+GTL
Sbjct: 144 KLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTL 203

Query: 234 FRKYGLAADNVIDAKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPS 293
            RKYGLA DNV+D K++D NG+++DR +MGEDLFWAIRGGGGASFG++L+WK+KLV VP 
Sbjct: 204 MRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPK 263

Query: 294 IVTVFNVQRSLEQGA-THLFQKWQTIAHKLHQDIFLHVTTKVTNFPSKKTIKLSFTSLFL 353
            VTVF V ++LEQ A      KWQ I+ K+ ++I + V  +       KT+ +++   FL
Sbjct: 264 TVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGNDGNKTVTMTYLGQFL 323

Query: 354 GPIERLIPLMNSHFPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESF 413
           G    L+ +M   FPELGL + DCTEMSWI++ LF     T +P+E+L++  S P+ + +
Sbjct: 324 GEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKS-PLGKDY 383

Query: 414 FTAKSDYVTSPISQNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFG 473
           F A SD+V  PI   GL+G++ +L+E   + L  TPYGG MS+I ES  PFPHR G++F 
Sbjct: 384 FKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFK 443

Query: 474 IQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGR-NTSYE 533
           I Y A W +N   + + ++WI+E+Y YM PYVS +PR AY+NYRDLD G+N      ++ 
Sbjct: 444 ILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNAKVNFI 503

Query: 534 EAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIP 566
           EAK+WG KYF  NF+RLV++KTKVDP NFF +EQSIP
Sbjct: 504 EAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIP 537

BLAST of Lsi02G009660 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 526.9 bits (1356), Expect = 2.0e-149
Identity = 252/504 (50.00%), Postives = 354/504 (70.24%), Query Frame = 0

Query: 65  RESFLECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPF 124
           ++ F+ C    ++    +  +    T   + FS +L+ + +NLRFL  + PKP F+ +P 
Sbjct: 32  QDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPI 91

Query: 125 HEYHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAAPFIVLDLINLSSIIINIETET 184
           HE HVQA+I+C++K  + +RVRSGGHDYEGLSYVS    PFI++DL  +  + INI+  +
Sbjct: 92  HESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNS 151

Query: 185 ASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVID 244
           A V++GAT+GELYYRIAEKS +HGFPAG C ++G+GGHI+GG +G++ RKYGL ADNV+D
Sbjct: 152 AWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLD 211

Query: 245 AKIIDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRSLEQ 304
           AKI+D NG+++DR +MGED FWAIRGG G SFG+IL+WK+KLV VP  VTVF V ++L+Q
Sbjct: 212 AKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQ 271

Query: 305 G-ATHLFQKWQTIAHKLHQDIFLHVTTKVTNFPSKKTIKLSFTSLFLGPIERLIPLMNSH 364
                +  KWQ +A KL +++F+ V   V      KT+  S+ +LFLG    L+ +M   
Sbjct: 272 DVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKS 331

Query: 365 FPELGLKRIDCTEMSWIQSVLFFADLSTETPLEVLMERSSPPISESFFTAKSDYVTSPIS 424
           FPELGL   DC EMSW++S+ + +   T TP  VL++  S P  +  F AKSD+V +PI 
Sbjct: 332 FPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKS-PFPKVSFKAKSDFVKTPIP 391

Query: 425 QNGLEGLWSKLLEEEKSELILTPYGGKMSQISESKTPFPHRKGSIFGIQYLATW-DNANE 484
           ++GL+G++ KLL+E+   +I  PYGG M++I ES+ PFPHRKG +F +QY+ +W D+   
Sbjct: 392 ESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKR 451

Query: 485 TEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRN-YGRNTSYEEAKVWGLKYFSDN 544
             +H++WIR++Y+YM PYVS +PR AY+NYRDLDLGRN     T  ++A+VWG  YF +N
Sbjct: 452 PSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNN 511

Query: 545 FERLVRVKTKVDPSNFFWNEQSIP 566
           F RL+ +K KVDP NFF +EQSIP
Sbjct: 512 FNRLMMIKAKVDPENFFRHEQSIP 534