Lsi02G009650 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G009650 vs. ExPASy Swiss-Prot
Match: Q9FKV2 (Berberine bridge enzyme-like 23 OS=Arabidopsis thaliana OX=3702 GN=At5g44360 PE=2 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 4.7e-132
Identity = 231/420 (55.00%), Postives = 301/420 (71.67%), Query Frame = 0

Query: 12  GISYVSSQLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGSC 71
           G+SY+S    FI+LDL+N+R I+I++  ETA V  GAT+GELYY+IAK S IHGFPAG+C
Sbjct: 121 GLSYLSLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTC 180

Query: 72  PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMN-RNSMGEDLFWAIRGGGG 131
           P+VGVGGH SGGGFG + RK+GLAADNV+DA+ +D NGRI N R  MGEDLFWAIRGGG 
Sbjct: 181 PSVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGA 240

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVI 191
            SFGV+LSWK+KLV VP  VT F     L Q  T +  +WQ IA +L  ++F+ VI+ + 
Sbjct: 241 ASFGVVLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSI- 300

Query: 192 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
                    +  ++  +F + +LG I+KLIPLMN  FPELGL   +C EM+WI S+++F 
Sbjct: 301 ---------SGGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYF- 360

Query: 252 GFSIKTPPQILLKRSP-ISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYG 311
            +    P + LL R    ++++FKAKSDFV +PIP+IGLEG+W +  E E+  +I+ P G
Sbjct: 361 NWKKGQPLETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEVESPIMIMEPLG 420

Query: 312 GKMSQISDLETPFPHRRGNIFGIQYMVTW--ENANETYKHLSWIREVYAYMKPYVSKYPR 371
           GKM +I + ETPFPHRRGN++ IQYMV W  ++     KH++W+R +Y YM+ YVS  PR
Sbjct: 421 GKMYEIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPR 480

Query: 372 AAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPP 428
            AYLNYRDLDLG N G NTS+E AK+WG +Y+ SNF RL  VK K+DP+NFF NEQS+PP
Sbjct: 481 GAYLNYRDLDLGMNRGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPP 529

BLAST of Lsi02G009650 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 2.0e-130
Identity = 223/420 (53.10%), Postives = 302/420 (71.90%), Query Frame = 0

Query: 12  GISYVSS-QLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGS 71
           G+SYVS  +  F+++DL  LR I +DIE+ +A V  GA++GE+YYRI +KS IHGFPAG 
Sbjct: 121 GLSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGL 180

Query: 72  CPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGG 131
           C ++G+GGHI GG +G++ RK+GL ADNV+DA+I+D +G+I+NR +MGED+FWAIRGGGG
Sbjct: 181 CTSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGG 240

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVI 191
            SFGVIL+WK+KLV VP IVTVF V R+LEQ  T L  KWQ +A KL +D+F+ VI   I
Sbjct: 241 GSFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVI---I 300

Query: 192 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
              +K P +  +TI  S+   FLG   +L+ +M   FP+LGL + +C+E SWI+SV++ A
Sbjct: 301 QPTSKTPKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIA 360

Query: 252 GFSIKTPPQILLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGG 311
           GF    P + LL    +   +FKAKSD+V  PIP  GLEGLW KL+EE++   I  PYGG
Sbjct: 361 GFPSTAPSEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGG 420

Query: 312 KMSQISDLETPFPHRRGNIFGIQYMVTWENANET-YKHLSWIREVYAYMKPYVSKYPRAA 371
            M++I + ETPFPHR G +F IQ++  W++   +  KH+ W+RE+Y+YM+ YVSK PR+A
Sbjct: 421 MMAKIPETETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSA 480

Query: 372 YLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSL 430
           Y+NYRDLDLG N G+ +    A+ WG +Y+K NF+RLV +K K DP NFF +EQSIP  L
Sbjct: 481 YVNYRDLDLGMN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of Lsi02G009650 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 2.4e-128
Identity = 226/422 (53.55%), Postives = 302/422 (71.56%), Query Frame = 0

Query: 12  GISYVSS-QLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGS 71
           G+SYVS  +  F+++DL  LR I++DI+  +A VE GAT+GELYYRIA+KS  HGFPAG 
Sbjct: 123 GVSYVSQIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGV 182

Query: 72  CPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGG 131
            P++G+GGHI+GG +G+L RKYGLAADNV+DAKI+D NG++++R SMGEDLFWAIRGG G
Sbjct: 183 YPSLGIGGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSG 242

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGAT-HLFQKWQTIAHKLHQDIFLHVIIGV 191
            SFG+ILSWK+KLV VP  +TVF V ++ EQ  +  +  KWQ IA  L  ++FL V   V
Sbjct: 243 GSFGIILSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTV 302

Query: 192 IDQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFF 251
                 + N  +KT+ ++++  FLG    L+ +M   FPELGL + +CIEMSWI S+++ 
Sbjct: 303 ------SGNKANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYN 362

Query: 252 AGFSIKTPPQI---LLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILT 311
           +GF    PP I   L  +SPI  ++FK KSDF   PIP +GLEG++ KL+EE+A+ +I T
Sbjct: 363 SGFPTNPPPPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWT 422

Query: 312 PYGGKMSQISDLETPFPHRRGNIFGIQYMVTWENANE-TYKHLSWIREVYAYMKPYVSKY 371
           PYGGKM +I + E PFPHR G  F IQY  +W ++ +   +   WIRE+Y YM PYVS  
Sbjct: 423 PYGGKMDKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSN 482

Query: 372 PRAAYLNYRDLDLGRNYGRNTS-YEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQS 427
           PR AY+NYRDLDLG+N   + S + +AK+WG  Y+K NF+RLVR+K+KVDP NFF +EQS
Sbjct: 483 PRQAYVNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQS 538

BLAST of Lsi02G009650 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.2e-127
Identity = 216/420 (51.43%), Postives = 297/420 (70.71%), Query Frame = 0

Query: 12  GISYVS-SQLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGS 71
           G+S+V+  +  F+++DL  LR +D+D+++ +A    GAT+GE+YYRI +KS  HGFPAG 
Sbjct: 119 GLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGL 178

Query: 72  CPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGG 131
           C ++G+GGH+ GG +G++ RK+GL ADNV+DA+I+D NG+I++R +MGED+FWAIRGGGG
Sbjct: 179 CSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGG 238

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVI 191
            SFGVIL+WK+KLV VP+ VTVF V ++LEQ  T +  KW+ IA KL  D+F+ VII   
Sbjct: 239 GSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPA 298

Query: 192 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
            +  K  N   +TI +S+ + FLG   +L+ +M   FPELGL + +C EMSWI+SV++ A
Sbjct: 299 SKTTKPGN---RTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA 358

Query: 252 GFSIKTPPQILLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGG 311
           GF     P+ LL    +    FKAKSDFV  PIP  GLEGLW + +EE++   I  PYGG
Sbjct: 359 GFPNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGG 418

Query: 312 KMSQISDLETPFPHRRGNIFGIQYMVTWENAN-ETYKHLSWIREVYAYMKPYVSKYPRAA 371
            MS+IS+ E PFPHR G +F IQ++ TW++      +H+ WIRE+Y+YM+ YVSK PR A
Sbjct: 419 MMSRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQA 478

Query: 372 YLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSL 430
           Y+NYRDLDLG N G       A+ WG KYYK NF+RLV++K + DP NFF +EQS+P  +
Sbjct: 479 YVNYRDLDLGTNEGET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of Lsi02G009650 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.2e-127
Identity = 224/423 (52.96%), Postives = 304/423 (71.87%), Query Frame = 0

Query: 13  ISYVSS-QLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGSC 72
           +SYVS  +  FI+LDL  L+ I++DIE+ +A V+ GATLGELYYRIA+KS IHGFPAG C
Sbjct: 125 LSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLC 184

Query: 73  PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGGT 132
            +VG+GG+++GGG+GTL RKYGLA DNV+D K++D NG++++R +MGEDLFWAIRGGGG 
Sbjct: 185 TSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGA 244

Query: 133 SFGVILSWKLKLVSVPSIVTVFNVQRSLEQGA-THLFQKWQTIAHKLHQDIFLHVIIGVI 192
           SFG++L+WK+KLV VP  VTVF V ++LEQ A      KWQ I+ K+ ++I + V++   
Sbjct: 245 SFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVL--- 304

Query: 193 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 252
                A N  +KT+ ++++  FLG    L+ +M   FPELGL + +C EMSWI++ LF  
Sbjct: 305 ---RAAGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG 364

Query: 253 GFSIKTPPQILLK-RSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYG 312
           GF   +P +ILL+ +SP+   +FKA SDFV  PIP IGL+G++ +L+E   +FL  TPYG
Sbjct: 365 GFPTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYG 424

Query: 313 GKMSQISDLETPFPHRRGNIFGIQYMVTW-ENANETYKHLSWIREVYAYMKPYVSKYPRA 372
           G MS+I +   PFPHR G +F I Y   W EN   + + ++WI+E+Y YM PYVS  PR 
Sbjct: 425 GMMSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQ 484

Query: 373 AYLNYRDLDLGRNYGR-NTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPP 431
           AY+NYRDLD G+N      ++ +AK+WG KY+K NFDRLV++KTKVDP NFF +EQSIPP
Sbjct: 485 AYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPP 541

BLAST of Lsi02G009650 vs. ExPASy TrEMBL
Match: A0A1S3BH69 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103489514 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 9.0e-211
Identity = 357/423 (84.40%), Postives = 389/423 (91.96%), Query Frame = 0

Query: 12  GISYVSS-QLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGS 71
           GISYVSS Q QFI+LD IN R IDID++TETA VE GATLGELYYRIA+KSPIHGFPAGS
Sbjct: 124 GISYVSSDQSQFILLDFINFRSIDIDMKTETAIVEAGATLGELYYRIAEKSPIHGFPAGS 183

Query: 72  CPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGG 131
           CPTVG+GGH+ GGGFGTLFRKYGLAADNVIDAKI+D NGRIM+RNSMGEDLFWAIRGGGG
Sbjct: 184 CPTVGMGGHVGGGGFGTLFRKYGLAADNVIDAKIVDVNGRIMDRNSMGEDLFWAIRGGGG 243

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVI 191
            SFGVILSWKLKLV VPS VTVF VQ++LEQGA HLFQKWQ IAHKLH+D+FLHV +GVI
Sbjct: 244 ASFGVILSWKLKLVYVPSNVTVFVVQKTLEQGAIHLFQKWQRIAHKLHEDLFLHVTMGVI 303

Query: 192 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
           D+QNK PN +SKTIL+SFVSLFLG IE+LIPLMNSHFPELGL+RNNC EMSWIQSVLFFA
Sbjct: 304 DEQNKTPNMSSKTILISFVSLFLGRIERLIPLMNSHFPELGLERNNCTEMSWIQSVLFFA 363

Query: 252 GFSIKTPPQILLKRSPISN-IFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYG 311
           G SI+TPP++LLKR P S  +FFKAKSDFV SPIPQIG EGLW K++EEEAS+LILTPYG
Sbjct: 364 GISIETPPKVLLKRPPTSKMLFFKAKSDFVISPIPQIGFEGLWTKMLEEEASYLILTPYG 423

Query: 312 GKMSQISDLETPFPHRRGNIFGIQYMVTWENANETYKHLSWIREVYAYMKPYVSKYPRAA 371
           GKM QISD +TPFPHR+GNIFGIQYMVTWENANETY+HLSWIREVY YM+PYVSK+PRAA
Sbjct: 424 GKMRQISDFDTPFPHRKGNIFGIQYMVTWENANETYRHLSWIREVYDYMEPYVSKHPRAA 483

Query: 372 YLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSL 431
           YLNYRDLDLGRN GRNTSYE+AKVWGLKYYKSNFDRLVRVKTKVDP NFFWNEQSIP S+
Sbjct: 484 YLNYRDLDLGRNCGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKVDPLNFFWNEQSIPTSV 543

Query: 432 YHS 433
           YHS
Sbjct: 544 YHS 546

BLAST of Lsi02G009650 vs. ExPASy TrEMBL
Match: A0A5A7U0X4 (Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold437G00100 PE=3 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 2.9e-201
Identity = 337/397 (84.89%), Postives = 368/397 (92.70%), Query Frame = 0

Query: 37  IETETASVETGATLGELYYRIAKKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAA 96
           ++TETA VE GATLGELYYRIA+KSPIHGFPAGSCPTVG+GGH+ GGGFGTLFRKYGLAA
Sbjct: 1   MKTETAIVEAGATLGELYYRIAEKSPIHGFPAGSCPTVGMGGHVGGGGFGTLFRKYGLAA 60

Query: 97  DNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGGTSFGVILSWKLKLVSVPSIVTVFNVQ 156
           DNVIDAKI+D NGRIM+RNSMGEDLFWAIRGGGG SFGVILSWKLKLV VPS VTVF VQ
Sbjct: 61  DNVIDAKIVDVNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVYVPSNVTVFVVQ 120

Query: 157 RSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVIDQQNKAPNATSKTILLSFVSLFLGPI 216
           ++LEQGA HLFQKWQ IAHKLH+D+FLHV +GVID+QNK PN +SKTIL+SFVSLFLG I
Sbjct: 121 KTLEQGAIHLFQKWQRIAHKLHEDLFLHVTMGVIDEQNKTPNMSSKTILISFVSLFLGRI 180

Query: 217 EKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFAGFSIKTPPQILLKRSPISN-IFFKAK 276
           E+LIPLMNSHFPELGL+RNNC EMSWIQSVLFFAG SI+TPP++LLKR P S  +FFKAK
Sbjct: 181 ERLIPLMNSHFPELGLERNNCTEMSWIQSVLFFAGISIETPPKVLLKRPPTSKMLFFKAK 240

Query: 277 SDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGGKMSQISDLETPFPHRRGNIFGIQYM 336
           SDFV SPIPQIG EGLW K++EEEAS+LILTPYGGKM QISD +TPFPHR+GNIFGIQYM
Sbjct: 241 SDFVISPIPQIGFEGLWTKMLEEEASYLILTPYGGKMRQISDFDTPFPHRKGNIFGIQYM 300

Query: 337 VTWENANETYKHLSWIREVYAYMKPYVSKYPRAAYLNYRDLDLGRNYGRNTSYEQAKVWG 396
           VTWENANETY+HLSWIREVY YM+PYVSK+PRAAYLNYRDLDLGRN GRNTSYE+AKVWG
Sbjct: 301 VTWENANETYRHLSWIREVYDYMEPYVSKHPRAAYLNYRDLDLGRNCGRNTSYEEAKVWG 360

Query: 397 LKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSLYHS 433
           LKYYKSNFDRLVRVKTKVDP NFFWNEQSIP S+YHS
Sbjct: 361 LKYYKSNFDRLVRVKTKVDPLNFFWNEQSIPTSVYHS 397

BLAST of Lsi02G009650 vs. ExPASy TrEMBL
Match: A0A6J1CGQ8 (berberine bridge enzyme-like 13 OS=Momordica charantia OX=3673 GN=LOC111010686 PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.3e-196
Identity = 339/422 (80.33%), Postives = 376/422 (89.10%), Query Frame = 0

Query: 12  GISYVSSQLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGSC 71
           G+SY SSQ  FIV+DLI+LR IDIDI+TETASVETGATLGELYYRIA+KSPIHGFPAGSC
Sbjct: 126 GLSYTSSQAPFIVIDLIHLRAIDIDIKTETASVETGATLGELYYRIAEKSPIHGFPAGSC 185

Query: 72  PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGGT 131
           PTVGVGGH+SGGGFGTLFRKYGLAADN+IDAKI+DF GRIM+RNSMGEDLFWAIRGGG  
Sbjct: 186 PTVGVGGHVSGGGFGTLFRKYGLAADNIIDAKIVDFKGRIMDRNSMGEDLFWAIRGGGXA 245

Query: 132 SFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVID 191
           SFGVILSWKLKLVSVPS VTVF VQ++LEQGAT LFQKWQTIA KL + +FLHVIIGV +
Sbjct: 246 SFGVILSWKLKLVSVPSKVTVFLVQKTLEQGATQLFQKWQTIADKLDEHLFLHVIIGVTE 305

Query: 192 QQNKAPNATS-KTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
            QNK+P   S KTILLSF SLFLGPI+KL  +M++HFPELGLQR N  EMSWIQSVLFF 
Sbjct: 306 NQNKSPKEPSTKTILLSFESLFLGPIDKLSLIMSTHFPELGLQRENYTEMSWIQSVLFFT 365

Query: 252 GFSIKTPPQILLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGG 311
           G S + P +ILL+R P+S+IFFKAKSD+VTSPIPQ GLE LW K++EEEAS LILTPYGG
Sbjct: 366 GLSTEAPLEILLERPPLSDIFFKAKSDYVTSPIPQTGLEALWSKVLEEEASLLILTPYGG 425

Query: 312 KMSQISDLETPFPHRRGNIFGIQYMVTWENANETYKHLSWIREVYAYMKPYVSKYPRAAY 371
           KMSQIS+ ETPFPHRRG IFGIQYMVTWEN NET KHLSW+REVY  M+ YVSK PR+AY
Sbjct: 426 KMSQISESETPFPHRRGIIFGIQYMVTWENGNETEKHLSWMREVYTCMELYVSKCPRSAY 485

Query: 372 LNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSLY 431
           L+YRDLDLGRNYGRN SYE+AKVWGLKY+K NF+RLVRVKTKVDPSN FWNEQSIPP+L+
Sbjct: 486 LSYRDLDLGRNYGRNRSYEEAKVWGLKYFKGNFERLVRVKTKVDPSNLFWNEQSIPPTLH 545

Query: 432 HS 433
           HS
Sbjct: 546 HS 547

BLAST of Lsi02G009650 vs. ExPASy TrEMBL
Match: A0A6J1KH65 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111495709 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 6.2e-196
Identity = 338/421 (80.29%), Postives = 376/421 (89.31%), Query Frame = 0

Query: 12  GISYVSSQLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGSC 71
           G+SY+SSQ QFIVLDL NLR ID+DIETETASVETGATLGE YYRIA+KS IHGFPAGSC
Sbjct: 110 GLSYLSSQSQFIVLDLTNLRSIDVDIETETASVETGATLGEFYYRIAEKSLIHGFPAGSC 169

Query: 72  PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGGT 131
           PTVGVGGHISGGGFGTLFRK+GLAAD VIDAKI+DFNGRIM+RN+MGE+LFWAIRGGGG 
Sbjct: 170 PTVGVGGHISGGGFGTLFRKHGLAADKVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGA 229

Query: 132 SFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVID 191
           SFGVILSWKLKLVS+P  +T+F+VQ++ +QGA  LFQKWQ IA+KL +D+FLHV IGV +
Sbjct: 230 SFGVILSWKLKLVSLPPKLTIFHVQKTPKQGAMQLFQKWQRIAYKLDEDLFLHVTIGVQE 289

Query: 192 QQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFAG 251
           Q          TI LSFV+LFLGPIEKLIPLMN+ FPELGLQR N  EM+WIQS+LFFAG
Sbjct: 290 Q----------TISLSFVALFLGPIEKLIPLMNTQFPELGLQRENYTEMNWIQSILFFAG 349

Query: 252 FSIKTPPQILLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGGK 311
           F I+TPPQILLK+ P+SN FFKAKSDFVT PIPQ GLEGLW K++EEE S+LILTPYGGK
Sbjct: 350 FPIQTPPQILLKK-PLSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGK 409

Query: 312 MSQISDLETPFPHRRGNIFGIQYMVTWENANETYKHLSWIREVYAYMKPYVSKYPRAAYL 371
           MSQIS+LETPFPHR+GNIFGIQYMV+WEN +E+YKHLSWIREVYAYM+PYVSK PR AYL
Sbjct: 410 MSQISELETPFPHRKGNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYL 469

Query: 372 NYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSLYH 431
           NYRDLDLGRNYGRN  YE+AKVWGLKYYKSNF+RLVRVKTKVDPSNFFWNEQSIP SLY 
Sbjct: 470 NYRDLDLGRNYGRNVRYEEAKVWGLKYYKSNFERLVRVKTKVDPSNFFWNEQSIPTSLYR 519

Query: 432 S 433
           S
Sbjct: 530 S 519

BLAST of Lsi02G009650 vs. ExPASy TrEMBL
Match: A0A6J1CHF0 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 6.2e-196
Identity = 336/424 (79.25%), Postives = 374/424 (88.21%), Query Frame = 0

Query: 12  GISYVSSQLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGSC 71
           G+SYVSSQ  FIV+DLINL  I IDIETETASVETGATLGE+YYRIA+KSPIHGFPAGSC
Sbjct: 115 GLSYVSSQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPIHGFPAGSC 174

Query: 72  PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGGT 131
           PTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKI+DFNGRI +RNSMGEDLFWAIRGGGG 
Sbjct: 175 PTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLFWAIRGGGGA 234

Query: 132 SFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVID 191
           SFGVILSWKLKLVSVP  VTVFNV R+L+QGATHLFQKWQ IAHK+ QD+FLHV   V+D
Sbjct: 235 SFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLFLHVTTKVVD 294

Query: 192 QQNK---APNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLF 251
            + K   +  A+SKTI LSF SLFLGPIE+LIPLMN+HF ELGL+RN+C EMSWIQSVLF
Sbjct: 295 HKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTEMSWIQSVLF 354

Query: 252 FAGFSIKTPPQILLKRS-PISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTP 311
           FA  S +TPP+IL++RS PIS+ FF AKSD+VTSPI Q GLE LW KL+EEE S LILTP
Sbjct: 355 FADLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEEEKSELILTP 414

Query: 312 YGGKMSQISDLETPFPHRRGNIFGIQYMVTWENANETYKHLSWIREVYAYMKPYVSKYPR 371
           YGGKM QIS+ ETPFPHRRGNI+GIQY+  WENANET KHLSW+REVY+YM+P+VSKYPR
Sbjct: 415 YGGKMDQISESETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYMQPFVSKYPR 474

Query: 372 AAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPP 431
            AYLNYRDLDLGRNY RNTSY++AKVWGLKY+K NF+RLVRVKTKVDP NFFWNEQSIP 
Sbjct: 475 GAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNFFWNEQSIPL 534

BLAST of Lsi02G009650 vs. NCBI nr
Match: XP_004135725.2 (berberine bridge enzyme-like 15 [Cucumis sativus] >KAE8653307.1 hypothetical protein Csa_023228 [Cucumis sativus])

HSP 1 Score: 750.7 bits (1937), Expect = 6.8e-213
Identity = 359/423 (84.87%), Postives = 394/423 (93.14%), Query Frame = 0

Query: 12  GISYVSS-QLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGS 71
           GISYVSS Q QFI+LDL N R IDID++TETA+VE GATLGELYYRIA+KSP HGFPAG+
Sbjct: 124 GISYVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGT 183

Query: 72  CPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGG 131
           CPTVG+GGH+SGGGFGTLFRKYGLAADNVIDAKI+DFNGRIM+RNSMGEDLFWAIRGGGG
Sbjct: 184 CPTVGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGG 243

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVI 191
            SFGVILSWKLKLV VPS VTVF VQ++LEQGA HLFQKWQTIAHKLH+D+FLHV IGVI
Sbjct: 244 ASFGVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVI 303

Query: 192 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
           D+QNK PN +SKTIL+SFVSLFLGP+E+LIPLMNSHFPELGL+RNNC EMSWIQSVL+FA
Sbjct: 304 DEQNKTPNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFA 363

Query: 252 GFSIKTPPQILLKRSPISNI-FFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYG 311
           G SI+ PP+ILLKR PISN+ FFKAKSDFV SPIPQIGLEGLW K++EE ASFLIL+PYG
Sbjct: 364 GISIEAPPEILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYG 423

Query: 312 GKMSQISDLETPFPHRRGNIFGIQYMVTWENANETYKHLSWIREVYAYMKPYVSKYPRAA 371
           GKM QISDLETPFPHR+GN FGIQY+VTWENANETY+HLSWIREVY YM+PYVSKYPRAA
Sbjct: 424 GKMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYPRAA 483

Query: 372 YLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSL 431
           YLNYRDLDLGRN GRNTSYE+AKVWGLKY+K+NFDRLVRVKTKVDP NFFWNEQSIP S+
Sbjct: 484 YLNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSI 543

Query: 432 YHS 433
           YHS
Sbjct: 544 YHS 546

BLAST of Lsi02G009650 vs. NCBI nr
Match: XP_008446963.1 (PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo])

HSP 1 Score: 742.7 bits (1916), Expect = 1.8e-210
Identity = 357/423 (84.40%), Postives = 389/423 (91.96%), Query Frame = 0

Query: 12  GISYVSS-QLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGS 71
           GISYVSS Q QFI+LD IN R IDID++TETA VE GATLGELYYRIA+KSPIHGFPAGS
Sbjct: 124 GISYVSSDQSQFILLDFINFRSIDIDMKTETAIVEAGATLGELYYRIAEKSPIHGFPAGS 183

Query: 72  CPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGG 131
           CPTVG+GGH+ GGGFGTLFRKYGLAADNVIDAKI+D NGRIM+RNSMGEDLFWAIRGGGG
Sbjct: 184 CPTVGMGGHVGGGGFGTLFRKYGLAADNVIDAKIVDVNGRIMDRNSMGEDLFWAIRGGGG 243

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVI 191
            SFGVILSWKLKLV VPS VTVF VQ++LEQGA HLFQKWQ IAHKLH+D+FLHV +GVI
Sbjct: 244 ASFGVILSWKLKLVYVPSNVTVFVVQKTLEQGAIHLFQKWQRIAHKLHEDLFLHVTMGVI 303

Query: 192 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
           D+QNK PN +SKTIL+SFVSLFLG IE+LIPLMNSHFPELGL+RNNC EMSWIQSVLFFA
Sbjct: 304 DEQNKTPNMSSKTILISFVSLFLGRIERLIPLMNSHFPELGLERNNCTEMSWIQSVLFFA 363

Query: 252 GFSIKTPPQILLKRSPISN-IFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYG 311
           G SI+TPP++LLKR P S  +FFKAKSDFV SPIPQIG EGLW K++EEEAS+LILTPYG
Sbjct: 364 GISIETPPKVLLKRPPTSKMLFFKAKSDFVISPIPQIGFEGLWTKMLEEEASYLILTPYG 423

Query: 312 GKMSQISDLETPFPHRRGNIFGIQYMVTWENANETYKHLSWIREVYAYMKPYVSKYPRAA 371
           GKM QISD +TPFPHR+GNIFGIQYMVTWENANETY+HLSWIREVY YM+PYVSK+PRAA
Sbjct: 424 GKMRQISDFDTPFPHRKGNIFGIQYMVTWENANETYRHLSWIREVYDYMEPYVSKHPRAA 483

Query: 372 YLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSL 431
           YLNYRDLDLGRN GRNTSYE+AKVWGLKYYKSNFDRLVRVKTKVDP NFFWNEQSIP S+
Sbjct: 484 YLNYRDLDLGRNCGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKVDPLNFFWNEQSIPTSV 543

Query: 432 YHS 433
           YHS
Sbjct: 544 YHS 546

BLAST of Lsi02G009650 vs. NCBI nr
Match: KAA0047129.1 (flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa] >TYK14946.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa])

HSP 1 Score: 711.1 bits (1834), Expect = 6.0e-201
Identity = 337/397 (84.89%), Postives = 368/397 (92.70%), Query Frame = 0

Query: 37  IETETASVETGATLGELYYRIAKKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAA 96
           ++TETA VE GATLGELYYRIA+KSPIHGFPAGSCPTVG+GGH+ GGGFGTLFRKYGLAA
Sbjct: 1   MKTETAIVEAGATLGELYYRIAEKSPIHGFPAGSCPTVGMGGHVGGGGFGTLFRKYGLAA 60

Query: 97  DNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGGTSFGVILSWKLKLVSVPSIVTVFNVQ 156
           DNVIDAKI+D NGRIM+RNSMGEDLFWAIRGGGG SFGVILSWKLKLV VPS VTVF VQ
Sbjct: 61  DNVIDAKIVDVNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVYVPSNVTVFVVQ 120

Query: 157 RSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVIDQQNKAPNATSKTILLSFVSLFLGPI 216
           ++LEQGA HLFQKWQ IAHKLH+D+FLHV +GVID+QNK PN +SKTIL+SFVSLFLG I
Sbjct: 121 KTLEQGAIHLFQKWQRIAHKLHEDLFLHVTMGVIDEQNKTPNMSSKTILISFVSLFLGRI 180

Query: 217 EKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFAGFSIKTPPQILLKRSPISN-IFFKAK 276
           E+LIPLMNSHFPELGL+RNNC EMSWIQSVLFFAG SI+TPP++LLKR P S  +FFKAK
Sbjct: 181 ERLIPLMNSHFPELGLERNNCTEMSWIQSVLFFAGISIETPPKVLLKRPPTSKMLFFKAK 240

Query: 277 SDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGGKMSQISDLETPFPHRRGNIFGIQYM 336
           SDFV SPIPQIG EGLW K++EEEAS+LILTPYGGKM QISD +TPFPHR+GNIFGIQYM
Sbjct: 241 SDFVISPIPQIGFEGLWTKMLEEEASYLILTPYGGKMRQISDFDTPFPHRKGNIFGIQYM 300

Query: 337 VTWENANETYKHLSWIREVYAYMKPYVSKYPRAAYLNYRDLDLGRNYGRNTSYEQAKVWG 396
           VTWENANETY+HLSWIREVY YM+PYVSK+PRAAYLNYRDLDLGRN GRNTSYE+AKVWG
Sbjct: 301 VTWENANETYRHLSWIREVYDYMEPYVSKHPRAAYLNYRDLDLGRNCGRNTSYEEAKVWG 360

Query: 397 LKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSLYHS 433
           LKYYKSNFDRLVRVKTKVDP NFFWNEQSIP S+YHS
Sbjct: 361 LKYYKSNFDRLVRVKTKVDPLNFFWNEQSIPTSVYHS 397

BLAST of Lsi02G009650 vs. NCBI nr
Match: XP_022139883.1 (berberine bridge enzyme-like 13 [Momordica charantia])

HSP 1 Score: 695.7 bits (1794), Expect = 2.6e-196
Identity = 339/422 (80.33%), Postives = 376/422 (89.10%), Query Frame = 0

Query: 12  GISYVSSQLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGSC 71
           G+SY SSQ  FIV+DLI+LR IDIDI+TETASVETGATLGELYYRIA+KSPIHGFPAGSC
Sbjct: 126 GLSYTSSQAPFIVIDLIHLRAIDIDIKTETASVETGATLGELYYRIAEKSPIHGFPAGSC 185

Query: 72  PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGGT 131
           PTVGVGGH+SGGGFGTLFRKYGLAADN+IDAKI+DF GRIM+RNSMGEDLFWAIRGGG  
Sbjct: 186 PTVGVGGHVSGGGFGTLFRKYGLAADNIIDAKIVDFKGRIMDRNSMGEDLFWAIRGGGXA 245

Query: 132 SFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVID 191
           SFGVILSWKLKLVSVPS VTVF VQ++LEQGAT LFQKWQTIA KL + +FLHVIIGV +
Sbjct: 246 SFGVILSWKLKLVSVPSKVTVFLVQKTLEQGATQLFQKWQTIADKLDEHLFLHVIIGVTE 305

Query: 192 QQNKAPNATS-KTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
            QNK+P   S KTILLSF SLFLGPI+KL  +M++HFPELGLQR N  EMSWIQSVLFF 
Sbjct: 306 NQNKSPKEPSTKTILLSFESLFLGPIDKLSLIMSTHFPELGLQRENYTEMSWIQSVLFFT 365

Query: 252 GFSIKTPPQILLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGG 311
           G S + P +ILL+R P+S+IFFKAKSD+VTSPIPQ GLE LW K++EEEAS LILTPYGG
Sbjct: 366 GLSTEAPLEILLERPPLSDIFFKAKSDYVTSPIPQTGLEALWSKVLEEEASLLILTPYGG 425

Query: 312 KMSQISDLETPFPHRRGNIFGIQYMVTWENANETYKHLSWIREVYAYMKPYVSKYPRAAY 371
           KMSQIS+ ETPFPHRRG IFGIQYMVTWEN NET KHLSW+REVY  M+ YVSK PR+AY
Sbjct: 426 KMSQISESETPFPHRRGIIFGIQYMVTWENGNETEKHLSWMREVYTCMELYVSKCPRSAY 485

Query: 372 LNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSLY 431
           L+YRDLDLGRNYGRN SYE+AKVWGLKY+K NF+RLVRVKTKVDPSN FWNEQSIPP+L+
Sbjct: 486 LSYRDLDLGRNYGRNRSYEEAKVWGLKYFKGNFERLVRVKTKVDPSNLFWNEQSIPPTLH 545

Query: 432 HS 433
           HS
Sbjct: 546 HS 547

BLAST of Lsi02G009650 vs. NCBI nr
Match: XP_023520227.1 (berberine bridge enzyme-like 15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 695.7 bits (1794), Expect = 2.6e-196
Identity = 340/421 (80.76%), Postives = 377/421 (89.55%), Query Frame = 0

Query: 12  GISYVSSQLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGSC 71
           G+SYVSSQ QFIVLDL NLR IDIDIETETASVETGATLGELYYRIA+KS IHGFPAGSC
Sbjct: 110 GLSYVSSQSQFIVLDLTNLRSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSC 169

Query: 72  PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGGT 131
           PTVGVGGHISGGGFGTLFRK+GLAADNVIDAKI+DFNGRIM+RN+MGE+LFWAIRGGGG 
Sbjct: 170 PTVGVGGHISGGGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNAMGEELFWAIRGGGGA 229

Query: 132 SFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVID 191
           SFGVILSWKLKLVS+P  +T+F+VQ++ +QGA  LFQKWQ IA+KL +D+FLHV IGV +
Sbjct: 230 SFGVILSWKLKLVSLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQE 289

Query: 192 QQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFAG 251
           Q          TI LSFV+LFLGPIEKLIPLMN+ FPELGLQR N  E++WIQS+LFFAG
Sbjct: 290 Q----------TISLSFVALFLGPIEKLIPLMNTQFPELGLQRENYTELNWIQSILFFAG 349

Query: 252 FSIKTPPQILLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGGK 311
           F I+TPPQILLK+S +SN FFKAKSDFVT PIPQ GLEGLW K++EEE S+LILTPYGGK
Sbjct: 350 FPIQTPPQILLKKS-LSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGK 409

Query: 312 MSQISDLETPFPHRRGNIFGIQYMVTWENANETYKHLSWIREVYAYMKPYVSKYPRAAYL 371
           MSQIS LETPFPHR+GNIFGIQYMV+WEN +E+YKHLSWIREVYAYM+PYVSK PR AYL
Sbjct: 410 MSQISSLETPFPHRKGNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYL 469

Query: 372 NYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSLYH 431
           NYRDLDLGRNYGRN  YE+AKVWGLKYY+SNF+RLVRVKTKVDP NFFWNEQSIPPSLY 
Sbjct: 470 NYRDLDLGRNYGRNVRYEEAKVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYR 519

Query: 432 S 433
           S
Sbjct: 530 S 519

BLAST of Lsi02G009650 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 472.6 bits (1215), Expect = 3.3e-133
Identity = 231/420 (55.00%), Postives = 301/420 (71.67%), Query Frame = 0

Query: 12  GISYVSSQLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGSC 71
           G+SY+S    FI+LDL+N+R I+I++  ETA V  GAT+GELYY+IAK S IHGFPAG+C
Sbjct: 121 GLSYLSLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTC 180

Query: 72  PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMN-RNSMGEDLFWAIRGGGG 131
           P+VGVGGH SGGGFG + RK+GLAADNV+DA+ +D NGRI N R  MGEDLFWAIRGGG 
Sbjct: 181 PSVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGA 240

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVI 191
            SFGV+LSWK+KLV VP  VT F     L Q  T +  +WQ IA +L  ++F+ VI+ + 
Sbjct: 241 ASFGVVLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSI- 300

Query: 192 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
                    +  ++  +F + +LG I+KLIPLMN  FPELGL   +C EM+WI S+++F 
Sbjct: 301 ---------SGGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYF- 360

Query: 252 GFSIKTPPQILLKRSP-ISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYG 311
            +    P + LL R    ++++FKAKSDFV +PIP+IGLEG+W +  E E+  +I+ P G
Sbjct: 361 NWKKGQPLETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEVESPIMIMEPLG 420

Query: 312 GKMSQISDLETPFPHRRGNIFGIQYMVTW--ENANETYKHLSWIREVYAYMKPYVSKYPR 371
           GKM +I + ETPFPHRRGN++ IQYMV W  ++     KH++W+R +Y YM+ YVS  PR
Sbjct: 421 GKMYEIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPR 480

Query: 372 AAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPP 428
            AYLNYRDLDLG N G NTS+E AK+WG +Y+ SNF RL  VK K+DP+NFF NEQS+PP
Sbjct: 481 GAYLNYRDLDLGMNRGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPP 529

BLAST of Lsi02G009650 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 467.2 bits (1201), Expect = 1.4e-131
Identity = 223/420 (53.10%), Postives = 302/420 (71.90%), Query Frame = 0

Query: 12  GISYVSS-QLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGS 71
           G+SYVS  +  F+++DL  LR I +DIE+ +A V  GA++GE+YYRI +KS IHGFPAG 
Sbjct: 121 GLSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGL 180

Query: 72  CPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGG 131
           C ++G+GGHI GG +G++ RK+GL ADNV+DA+I+D +G+I+NR +MGED+FWAIRGGGG
Sbjct: 181 CTSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGG 240

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVI 191
            SFGVIL+WK+KLV VP IVTVF V R+LEQ  T L  KWQ +A KL +D+F+ VI   I
Sbjct: 241 GSFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVI---I 300

Query: 192 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
              +K P +  +TI  S+   FLG   +L+ +M   FP+LGL + +C+E SWI+SV++ A
Sbjct: 301 QPTSKTPKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIA 360

Query: 252 GFSIKTPPQILLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGG 311
           GF    P + LL    +   +FKAKSD+V  PIP  GLEGLW KL+EE++   I  PYGG
Sbjct: 361 GFPSTAPSEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGG 420

Query: 312 KMSQISDLETPFPHRRGNIFGIQYMVTWENANET-YKHLSWIREVYAYMKPYVSKYPRAA 371
            M++I + ETPFPHR G +F IQ++  W++   +  KH+ W+RE+Y+YM+ YVSK PR+A
Sbjct: 421 MMAKIPETETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSA 480

Query: 372 YLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSL 430
           Y+NYRDLDLG N G+ +    A+ WG +Y+K NF+RLV +K K DP NFF +EQSIP  L
Sbjct: 481 YVNYRDLDLGMN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of Lsi02G009650 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 460.3 bits (1183), Expect = 1.7e-129
Identity = 226/422 (53.55%), Postives = 302/422 (71.56%), Query Frame = 0

Query: 12  GISYVSS-QLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGS 71
           G+SYVS  +  F+++DL  LR I++DI+  +A VE GAT+GELYYRIA+KS  HGFPAG 
Sbjct: 123 GVSYVSQIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGV 182

Query: 72  CPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGG 131
            P++G+GGHI+GG +G+L RKYGLAADNV+DAKI+D NG++++R SMGEDLFWAIRGG G
Sbjct: 183 YPSLGIGGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSG 242

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGAT-HLFQKWQTIAHKLHQDIFLHVIIGV 191
            SFG+ILSWK+KLV VP  +TVF V ++ EQ  +  +  KWQ IA  L  ++FL V   V
Sbjct: 243 GSFGIILSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTV 302

Query: 192 IDQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFF 251
                 + N  +KT+ ++++  FLG    L+ +M   FPELGL + +CIEMSWI S+++ 
Sbjct: 303 ------SGNKANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYN 362

Query: 252 AGFSIKTPPQI---LLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILT 311
           +GF    PP I   L  +SPI  ++FK KSDF   PIP +GLEG++ KL+EE+A+ +I T
Sbjct: 363 SGFPTNPPPPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWT 422

Query: 312 PYGGKMSQISDLETPFPHRRGNIFGIQYMVTWENANE-TYKHLSWIREVYAYMKPYVSKY 371
           PYGGKM +I + E PFPHR G  F IQY  +W ++ +   +   WIRE+Y YM PYVS  
Sbjct: 423 PYGGKMDKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSN 482

Query: 372 PRAAYLNYRDLDLGRNYGRNTS-YEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQS 427
           PR AY+NYRDLDLG+N   + S + +AK+WG  Y+K NF+RLVR+K+KVDP NFF +EQS
Sbjct: 483 PRQAYVNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQS 538

BLAST of Lsi02G009650 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 458.0 bits (1177), Expect = 8.5e-129
Identity = 216/420 (51.43%), Postives = 297/420 (70.71%), Query Frame = 0

Query: 12  GISYVS-SQLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGS 71
           G+S+V+  +  F+++DL  LR +D+D+++ +A    GAT+GE+YYRI +KS  HGFPAG 
Sbjct: 119 GLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGL 178

Query: 72  CPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGG 131
           C ++G+GGH+ GG +G++ RK+GL ADNV+DA+I+D NG+I++R +MGED+FWAIRGGGG
Sbjct: 179 CSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGG 238

Query: 132 TSFGVILSWKLKLVSVPSIVTVFNVQRSLEQGATHLFQKWQTIAHKLHQDIFLHVIIGVI 191
            SFGVIL+WK+KLV VP+ VTVF V ++LEQ  T +  KW+ IA KL  D+F+ VII   
Sbjct: 239 GSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPA 298

Query: 192 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 251
            +  K  N   +TI +S+ + FLG   +L+ +M   FPELGL + +C EMSWI+SV++ A
Sbjct: 299 SKTTKPGN---RTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA 358

Query: 252 GFSIKTPPQILLKRSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYGG 311
           GF     P+ LL    +    FKAKSDFV  PIP  GLEGLW + +EE++   I  PYGG
Sbjct: 359 GFPNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGG 418

Query: 312 KMSQISDLETPFPHRRGNIFGIQYMVTWENAN-ETYKHLSWIREVYAYMKPYVSKYPRAA 371
            MS+IS+ E PFPHR G +F IQ++ TW++      +H+ WIRE+Y+YM+ YVSK PR A
Sbjct: 419 MMSRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQA 478

Query: 372 YLNYRDLDLGRNYGRNTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPPSL 430
           Y+NYRDLDLG N G       A+ WG KYYK NF+RLV++K + DP NFF +EQS+P  +
Sbjct: 479 YVNYRDLDLGTNEGET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of Lsi02G009650 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 458.0 bits (1177), Expect = 8.5e-129
Identity = 224/423 (52.96%), Postives = 304/423 (71.87%), Query Frame = 0

Query: 13  ISYVSS-QLQFIVLDLINLRGIDIDIETETASVETGATLGELYYRIAKKSPIHGFPAGSC 72
           +SYVS  +  FI+LDL  L+ I++DIE+ +A V+ GATLGELYYRIA+KS IHGFPAG C
Sbjct: 125 LSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLC 184

Query: 73  PTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMNRNSMGEDLFWAIRGGGGT 132
            +VG+GG+++GGG+GTL RKYGLA DNV+D K++D NG++++R +MGEDLFWAIRGGGG 
Sbjct: 185 TSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGA 244

Query: 133 SFGVILSWKLKLVSVPSIVTVFNVQRSLEQGA-THLFQKWQTIAHKLHQDIFLHVIIGVI 192
           SFG++L+WK+KLV VP  VTVF V ++LEQ A      KWQ I+ K+ ++I + V++   
Sbjct: 245 SFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVL--- 304

Query: 193 DQQNKAPNATSKTILLSFVSLFLGPIEKLIPLMNSHFPELGLQRNNCIEMSWIQSVLFFA 252
                A N  +KT+ ++++  FLG    L+ +M   FPELGL + +C EMSWI++ LF  
Sbjct: 305 ---RAAGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG 364

Query: 253 GFSIKTPPQILLK-RSPISNIFFKAKSDFVTSPIPQIGLEGLWIKLVEEEASFLILTPYG 312
           GF   +P +ILL+ +SP+   +FKA SDFV  PIP IGL+G++ +L+E   +FL  TPYG
Sbjct: 365 GFPTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYG 424

Query: 313 GKMSQISDLETPFPHRRGNIFGIQYMVTW-ENANETYKHLSWIREVYAYMKPYVSKYPRA 372
           G MS+I +   PFPHR G +F I Y   W EN   + + ++WI+E+Y YM PYVS  PR 
Sbjct: 425 GMMSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQ 484

Query: 373 AYLNYRDLDLGRNYGR-NTSYEQAKVWGLKYYKSNFDRLVRVKTKVDPSNFFWNEQSIPP 431
           AY+NYRDLD G+N      ++ +AK+WG KY+K NFDRLV++KTKVDP NFF +EQSIPP
Sbjct: 485 AYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPP 541