Lsi02G009020 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G009020
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotease Do-like 5, chloroplastic
Locationchr02: 8636150 .. 8640042 (+)
RNA-Seq ExpressionLsi02G009020
SyntenyLsi02G009020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAATCCACAGAACAGTCATCTCAACTGCCAAGGAAGATAATCGGATTGCTAGTTGAAGATTGGTTGAAGAAACAAAGTGGAAATCCATGGCGTTGGGCTCACTGGGACTTCGTCCTCTTCCAATTTCAGCTCCCCCAAATTCATCTGAAAATCCCTTACCCTTCACTTCCCGAAGAGCCATTGTGTTCGCCCCCACTGCTTTAATGGCTTCCCTGCTCGCTTTCCCTGTTCCCACTTACGCCGCTCTTCCCCAACTGCAAGACGACGTTCCACAAGAAGAAGATCGAATCGTTGCCCTTTTCCAGGTTCCTCTCTTTCTCTGAGAATTCTTCTGTTTCAATTCAAATTGGGTCTTGTTTTATCTGTTATGTTCTGTTGGTTCCCCCATTGCAATGTAGGAAACTTCGCCTTCCGTCGTTTACATTAAGGACCTTGAAATAGCTAAAAACCCTCAAAACCCTTCTCAAGAAGCCATGCTTATCGAGGATGAAAATGCCAAGGTCAAAGGGACTGGTTCCGGCTTTATTTGGGATAAATTTGGCCATATCGTAAGCCCTTTTCTTCTTTGATTCTTATATTTCTTGTTTTTGCCAACTCGATAATCCCCTAGAGAAGTTACCTATCTGAATGCACATTTTTAGCTGAAGCATTTCTGCTTGTGACAGGTCACTAATTACCATGTTGTTTCTGCATTGGCTACTGATAATAGTGGATTACAGCGTTGTAAGGTTTTGATTGTTTACTACTGTGTCTTGGATTGTTGTGCATTCTTAGTGGATGATATGATAGCAGTCTCTAGCCATGATTTCTCAGATTTCGGTCCTTCTGTAGGTAAATTTAGTCGATGTTAAAGGAAATGGAATCTATAAAGAAGCAAAGATTGTTGGTTTTGATCCAGAGTATGATCTAGCTGTTCTTAAGGTATTGAATCAAGCTTGCTTGTTCTTTTCTTTTCTTTTCTTTTCCAGCTAGTTTCTTTAAGCTTCTGGTTTGCTTTGTTATGTGAAAACTATGTGCTGTCAATTCAGTGAATGGCTTCACTCAGTTTCGACACTCATGAAATCCCTCCTGAAATTGGTGCCCATATTCAAATTCTATATCGATGTGCTAATTTAGCTCGAGAGTCGTGTCTAGAACACTTGTATCTTCATATCCTTCTAAAGGTGTCTGAGAAGCATGGATACAGATACGAGACACAAATACGACACAAACACAATGACATGTCATTTTTTAAAATTCTAAGACACAGATATGGAAAGAACATTTCTTAAATATGTATCCCTTTTAAAAGTCAACAAGTTTATTGTGCATTTATATGCTTTAAAAAATGAATTTGTTGTACTTCGACCTAAAGTCTTTACTTTTTGGTCCTATATTATTGTGTCTATTTAGTACACTTAACAAGTGTTTAGTACGTGTCTAAAAAATTATGAACTTGTATTGACTTTGTACATCTTGTGTCAAACACATATCTATTGTGCTAACAAGTGTTCGATATGTGTCCAAGTGTCTAAAACGATAGTTTGGATGTACGATGAAAAAGAAATTGAATTTGTATTTGAATCTTGGGTTCCGATCTCGGCTGAGAAGGACTGAGAAAAACTATTCCAACGAGTTCTCTAAAAAGGGTGCTAGTTGTGAAAGATGAAAACATTTGATGGTTATTTTTGACAATTTTCCAGTGATCTTCATCTCATTATCTATTCCAAATGTGGTGTGCAAATACGGCAGATTGGACAATCTATATTGTCAATCTCTTATGTCATACAATGATTTGCTGAGTTTGAATTTTCCATGTAACATCCAGGTGGAACTTGAAGGACATGAACTAAAGCCCATAGTCTTTGGTACCTCTCGAAATCTACGTGTTGGTCAGAGCTGCTATGCTATTGGCAACCCTTTTGGCTATGAGAAGACACTAACAGCAGGGGTATGACATCAATAATGTTACTTCCTCTATTTAATTTAGGGAAACCCTCTTTGAGTTTTCCATTCCAAAGCTAAAACTAAATTCCACAAGGAAATACTTCTTTCTTTCATTGAGCATATTAATCATTGAAGGTATGATATCTCTCATCTCACACACATTTATGCTTTATTTACCAAAGAACGTGGATGAAGTAAGAGTTTCTCTAAGTGTCACTTTCAAGTTTCCTCCTGGTTTTCTTTACCAAGTAACACAGAGTCATATAACAGATTGACTAAATAATCGAGAATCGTAAATCATTCAGGGTTTGGATAGCAGGTGATCAGCGGATTGGGTAGAGAAATTCCATCGCCAAATGGAAGGGCCATCAGGGGAGCTATTCAGACAGATGCTGCTATTAGTGCAGGTTCATGTTTCAACTACCTGAAATAAGATTTTGCCCTTTCAAACACCATTATCTTTTCATGTTCTTCCTTCTTCCTCTTCTCAAAGTTATTTTACTTTCATCACTCACATTGCAAGTGTCAGCCTTATAATAGTTATTGATTCCAGTGATCTTGATTCTTGATACTATTGGGCAGTTAAATTCCATGTACATTATACCGGCTTATCTTGATCTTTCTATTCTATCTCTGGTTCATTGCTGCCCCAAATCATCCATATTATAATAACTATGCCTGAACTTATCACATTTTAATACATAAATTTTTTACAGGGAATTCAGGGGGGCCTTTGGTTGACTCATACGGTCATGTTATTGGAGTCAACACAGCAACTTTCACTCGGAAAGGTGAAGGATCTCAATTTCTTTATCTGCCTTAGTTTGTCACAGGAAATATGAATGCATAATGTTGCCTACTTATGCTTTAAATGTGGCTGATTTCATCAAAGCTAATTCATTTTTTTCATAGCCACCTGAAAGAACAGTCTTCACAATAGTAAAGTAAAGAGAGAACGAACAAATAATGGTGTATTTTTTGTGGTTATTGAGTTCAAAGTTGTGTTTTTTCATGTTTGGTTATGCACTGCAGGAACTGGAATGTCTTCTGGGGTTAATTTTGCTATACCTATAGACACAGTTGTACGGACTGTGCCATACCTTATTGTGTATGGAACACCTTACAGTGAGAGATTTTGAACTAAGTTTTACTATCTCAAGGGAACACAGCAGGCATTCATGCAGCTCCAACTCCTCTCCAAGACCATAGACCACATTGTAAATGACACTTTGCTAATATAATGAATTTGCTTTCTCTAGTGTATAATCCTTATGCATTTCAAGATTTTGGTCTGGATTGTGAATAATTATTTACTGAGTATTATTCTTCCATCTCTCTGTTGGTTAATTCTATCATTGAACTTACTATTTATTAAATGCAATGGGAAGAGTTTGAATGATTGCAACCATCATGGGTTGGCCTAGTGATAAAAAAGGAGACAAAGTCTCAATAAATAATTAAGAGATCATGGGTTCAATTCATGGTGGCTACCTACCTAGGAATTAATTTCCTACGAGTTTCCTTGACACCCAAATGTTGTAGGGTCAGGCGGGTCTCCCGTGACATTAGTCGAGGTGCGCGTGGTGTTGTGGTGGCGGCATAAATGGAATTCAAGTCAGGCTGGGTGGAGGCGGAAGCTGGAGAGGTGTTGGCTTTGTTGTCGGGCCTGAGACTTGCTGAGGAGCTGGGTGTTTCATCTCTGTGGGTTGAAGCCGACGCTCTGGTGGTGGTGAAAAATGTCTAATCTATGAGCTTTATCTTATCTCCATTTAGAATAATTTATGTTGAAATTTTTAATTTGTTAAAGTTAATGTTGTGGGACTTTGTCATGCTCCTAGAGCTTGTAATCAGGTGGCCCATCAGTTGGCCACCCATGCTTTTAATATTTCGGTGTGTTGTCATTGAATTGATTCTCTTCTCCGTTGGCTTTCAACCTTTGTTGGGAAGGAGATAGTGACTTCTAA

mRNA sequence

AAAAAATCCACAGAACAGTCATCTCAACTGCCAAGGAAGATAATCGGATTGCTAGTTGAAGATTGGTTGAAGAAACAAAGTGGAAATCCATGGCGTTGGGCTCACTGGGACTTCGTCCTCTTCCAATTTCAGCTCCCCCAAATTCATCTGAAAATCCCTTACCCTTCACTTCCCGAAGAGCCATTGTGTTCGCCCCCACTGCTTTAATGGCTTCCCTGCTCGCTTTCCCTGTTCCCACTTACGCCGCTCTTCCCCAACTGCAAGACGACGTTCCACAAGAAGAAGATCGAATCGTTGCCCTTTTCCAGGAAACTTCGCCTTCCGTCGTTTACATTAAGGACCTTGAAATAGCTAAAAACCCTCAAAACCCTTCTCAAGAAGCCATGCTTATCGAGGATGAAAATGCCAAGGTCAAAGGGACTGGTTCCGGCTTTATTTGGGATAAATTTGGCCATATCGTCACTAATTACCATGTTGTTTCTGCATTGGCTACTGATAATAGTGGATTACAGCGTTGTAAGGTAAATTTAGTCGATGTTAAAGGAAATGGAATCTATAAAGAAGCAAAGATTGTTGGTTTTGATCCAGAGTATGATCTAGCTGTTCTTAAGGTGGAACTTGAAGGACATGAACTAAAGCCCATAGTCTTTGGTACCTCTCGAAATCTACGTGTTGGTCAGAGCTGCTATGCTATTGGCAACCCTTTTGGCTATGAGAAGACACTAACAGCAGGGGTGATCAGCGGATTGGGTAGAGAAATTCCATCGCCAAATGGAAGGGCCATCAGGGGAGCTATTCAGACAGATGCTGCTATTAGTGCAGGGAATTCAGGGGGGCCTTTGGTTGACTCATACGGTCATGTTATTGGAGTCAACACAGCAACTTTCACTCGGAAAGGAACTGGAATGTCTTCTGGGGTTAATTTTGCTATACCTATAGACACAGTTGTACGGACTGTGCCATACCTTATTGTGTATGGAACACCTTACAGTGAGAGATTTTGAACTAAGTTTTACTATCTCAAGGGAACACAGCAGGCATTCATGCAGCTCCAACTCCTCTCCAAGACCATAGACCACATTGGTCAGGCGGGTCTCCCGTGACATTAGTCGAGGTGCGCGTGGTGTTGTGGTGGCGGCATAAATGGAATTCAAGTCAGGCTGGGTGGAGGCGGAAGCTGGAGAGGTGTTGGCTTTGTTGTCGGGCCTGAGACTTGCTGAGGAGCTGGGTGTTTCATCTCTGTGGGTTGAAGCCGACGCTCTGGTGGTGGTGAAAAATGTCTAATCTATGAGCTTTATCTTATCTCCATTTAGAATAATTTATGTTGAAATTTTTAATTTGTTAAAGTTAATGTTGTGGGACTTTGTCATGCTCCTAGAGCTTGTAATCAGGTGGCCCATCAGTTGGCCACCCATGCTTTTAATATTTCGGTGTGTTGTCATTGAATTGATTCTCTTCTCCGTTGGCTTTCAACCTTTGTTGGGAAGGAGATAGTGACTTCTAA

Coding sequence (CDS)

ATGGCGTTGGGCTCACTGGGACTTCGTCCTCTTCCAATTTCAGCTCCCCCAAATTCATCTGAAAATCCCTTACCCTTCACTTCCCGAAGAGCCATTGTGTTCGCCCCCACTGCTTTAATGGCTTCCCTGCTCGCTTTCCCTGTTCCCACTTACGCCGCTCTTCCCCAACTGCAAGACGACGTTCCACAAGAAGAAGATCGAATCGTTGCCCTTTTCCAGGAAACTTCGCCTTCCGTCGTTTACATTAAGGACCTTGAAATAGCTAAAAACCCTCAAAACCCTTCTCAAGAAGCCATGCTTATCGAGGATGAAAATGCCAAGGTCAAAGGGACTGGTTCCGGCTTTATTTGGGATAAATTTGGCCATATCGTCACTAATTACCATGTTGTTTCTGCATTGGCTACTGATAATAGTGGATTACAGCGTTGTAAGGTAAATTTAGTCGATGTTAAAGGAAATGGAATCTATAAAGAAGCAAAGATTGTTGGTTTTGATCCAGAGTATGATCTAGCTGTTCTTAAGGTGGAACTTGAAGGACATGAACTAAAGCCCATAGTCTTTGGTACCTCTCGAAATCTACGTGTTGGTCAGAGCTGCTATGCTATTGGCAACCCTTTTGGCTATGAGAAGACACTAACAGCAGGGGTGATCAGCGGATTGGGTAGAGAAATTCCATCGCCAAATGGAAGGGCCATCAGGGGAGCTATTCAGACAGATGCTGCTATTAGTGCAGGGAATTCAGGGGGGCCTTTGGTTGACTCATACGGTCATGTTATTGGAGTCAACACAGCAACTTTCACTCGGAAAGGAACTGGAATGTCTTCTGGGGTTAATTTTGCTATACCTATAGACACAGTTGTACGGACTGTGCCATACCTTATTGTGTATGGAACACCTTACAGTGAGAGATTTTGA

Protein sequence

MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDDVPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGHELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Homology
[Close]
BLAST of Lsi02G009020 vs. ExPASy Swiss-Prot
Match: Q9SEL7 (Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 SV=3)

HSP 1 Score: 364.4 bits (934), Expect = 1.3e-99
Identity = 186/285 (65.26%), Postives = 225/285 (78.95%), Query Frame = 0 

Query: 29  RRAIVFAPT-ALMASLLA-----FPVPTYAALPQL---QDDVPQEEDRIVALFQETSPSV 88
           RR ++F  + AL +SLL       P+ +  AL Q    ++++ +EE+R V LFQ+TSPSV
Sbjct: 43  RRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNVNLFQKTSPSV 102

Query: 89  VYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKFGHIVTNYHVVSALATDNSG 148
           VYI+ +E+ K     S   +L ++EN K++GTGSGF+WDK GHIVTNYHV++ LATD  G
Sbjct: 103 VYIEAIELPKT----SSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHVIAKLATDQFG 162

Query: 149 LQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGHELKPIVFGTSRNLRVGQSC 208
           LQRCKV+LVD KG    KE KIVG DP+ DLAVLK+E EG EL P+V GTS +LRVGQSC
Sbjct: 163 LQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLGTSNDLRVGQSC 222

Query: 209 YAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVI 268
           +AIGNP+GYE TLT GV+SGLGREIPSPNG++I  AIQTDA I++GNSGGPL+DSYGH I
Sbjct: 223 FAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGNSGGPLLDSYGHTI 282

Query: 269 GVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305
           GVNTATFTRKG+GMSSGVNFAIPIDTVVRTVPYLIVYGT Y +RF
Sbjct: 283 GVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYRDRF 323

[Close]
BLAST of Lsi02G009020 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 194.1 bits (492), Expect = 2.3e-48
Identity = 125/291 (42.96%), Postives = 172/291 (59.11%), Query Frame = 0 

Query: 18  NSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQD----------DVPQEEDR 77
           +  ++ L FT   A+   P  L+ + +A     +AA P ++            +  +E  
Sbjct: 63  DDDDDTLHFTPFSAV--KPFFLLCTSVALSFSLFAASPAVESASAFVVSTPKKLQTDELA 122

Query: 78  IVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKFGHIVTNY 137
            V LFQE +PSVVYI +L +        Q+A  + D     +G+GSGF+WDK GHIVTNY
Sbjct: 123 TVRLFQENTPSVVYITNLAV-------RQDAFTL-DVLEVPQGSGSGFVWDKQGHIVTNY 182

Query: 138 HVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGHELKPIVF 197
           HV+        G    +V L D        +AK+VGFD + D+AVL+++   ++L+PI  
Sbjct: 183 HVI-------RGASDLRVTLAD----QTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPV 242

Query: 198 GTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPS-PNGRAIRGAIQTDAAISAGN 257
           G S +L VGQ  +AIGNPFG + TLT GVISGL REI S   GR I+  IQTDAAI+ GN
Sbjct: 243 GVSADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGN 302

Query: 258 SGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYG 298
           SGGPL+DS G +IG+NTA ++   +G SSGV F+IP+DTV   V  L+ +G
Sbjct: 303 SGGPLLDSSGTLIGINTAIYS--PSGASSGVGFSIPVDTVGGIVDQLVRFG 330

[Close]
BLAST of Lsi02G009020 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.5e-47
Identity = 131/297 (44.11%), Postives = 176/297 (59.26%), Query Frame = 0 

Query: 8   LRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDDV------ 67
           L  L + + P+++   L  +    + F P+  + S LA   P+ A +  +   V      
Sbjct: 54  LHELAVKSVPSTTRRILLTSLFMNLCFNPSRYL-SALALGDPSVATVEDVSPTVFPAGPL 113

Query: 68  PQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKFG 127
              E RIV LF++ + SVV I D+ +   PQ      + I +      G GSG +WD  G
Sbjct: 114 FPTEGRIVQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQG 173

Query: 128 HIVTNYHVV-SALATDNS-GLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 187
           +IVTNYHV+ +AL+ + S G    +VN++   G     E K+VG D   DLAVLKV+   
Sbjct: 174 YIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPE 233

Query: 188 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 247
             LKPI  G S +L+VGQ C AIGNPFG++ TLT GVISGL R+I S  G  I G IQTD
Sbjct: 234 TLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTD 293

Query: 248 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVY 297
           AAI+ GNSGGPL+DS G++IG+NTA FT+  TG S+GV FAIP  TV++ VP LI +
Sbjct: 294 AAINPGNSGGPLLDSKGNLIGINTAIFTQ--TGTSAGVGFAIPSSTVLKIVPQLIQF 339

[Close]
BLAST of Lsi02G009020 vs. ExPASy Swiss-Prot
Match: Q2SL36 (Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=mucD PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.2e-30
Identity = 81/181 (44.75%), Postives = 111/181 (61.33%), Query Frame = 0 

Query: 107 KVKGTGSGFIWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDP 166
           + + TGSGFI  K G+I+TN HVV       +G     V L+D +       AK++G D 
Sbjct: 87  EAQSTGSGFIVSKDGYILTNNHVV-------AGADEIFVRLMDRR----ELTAKLIGSDE 146

Query: 167 EYDLAVLKVELEGHELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPS 226
           + DLAVLKVE +  +L  +  G S  L+VG+   AIG+PFG+E T+TAG++S  GR +P+
Sbjct: 147 KSDLAVLKVEAD--DLPVLNLGKSSELKVGEWVVAIGSPFGFEYTVTAGIVSAKGRSLPN 206

Query: 227 PNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV 286
            N       IQTD AI+ GNSGGPL +  G V+G+N+  +TR G  M  GV+FAIPID  
Sbjct: 207 ENYVPF---IQTDVAINPGNSGGPLFNLEGEVVGINSQIYTRSGGFM--GVSFAIPIDVA 249

Query: 287 V 288
           +
Sbjct: 267 L 249

[Close]
BLAST of Lsi02G009020 vs. ExPASy Swiss-Prot
Match: Q4KGQ4 (Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) OX=220664 GN=mucD PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.4e-28
Identity = 86/240 (35.83%), Postives = 127/240 (52.92%), Query Frame = 0 

Query: 71  LFQETSPSVVYIK------DLEIAKNPQNPSQEA---MLIE--------------DENAK 130
           L ++ SP+VV I       D  ++ + Q P  E    ML E              D   +
Sbjct: 36  LVEQASPAVVNISTTQKLPDRRVSNSAQMPDLEGLPPMLREFFERGMPQPRSPRGDRQRE 95

Query: 131 VKGTGSGFIWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPE 190
            +  GSGFI    G+I+TN HV+       +      V L D        +AK++G DP 
Sbjct: 96  AQSLGSGFIISADGYILTNNHVI-------ADADEILVRLADRS----ELKAKLIGTDPR 155

Query: 191 YDLAVLKVELEGHELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSP 250
            D+A+LK+  +G +L  +  G S++L+ GQ   AIG+PFG++ T+T G++S +GR +P+ 
Sbjct: 156 SDVALLKI--DGKDLPVLKLGKSQDLKAGQWVVAIGSPFGFDHTVTQGIVSAIGRSLPNE 215

Query: 251 NGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVV 288
           N       IQTD  I+ GNSGGPL +  G V+G+N+  +TR G  M  GV+FAIPID  +
Sbjct: 216 NYVPF---IQTDVPINPGNSGGPLFNLAGEVVGINSQIYTRSGGFM--GVSFAIPIDVAM 257

[Close]
BLAST of Lsi02G009020 vs. ExPASy TrEMBL
Match: A0A6J1EKF8 (protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434145 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.4e-157
Identity = 286/305 (93.77%), Postives = 297/305 (97.38%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MALGSLG+R LP+S+PPNSSE  LPFTSRRAIVFAPTALMASLLAFPVP++AALPQLQD+
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIE-DENAKVKGTGSGFIWDK 120
           VPQEEDRIV LFQETSPSVVYIK+LEIAK PQNPS+EAMLIE DENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGI ++AKIVGFDPEYDLAVLKVELEG
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSR+LRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]
BLAST of Lsi02G009020 vs. ExPASy TrEMBL
Match: A0A5D3DAW0 (Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00660 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.4e-156
Identity = 283/305 (92.79%), Postives = 293/305 (96.07%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MALG LG+ PLPI APPNSSENPLPFTSRRAI+F+PTALMASLLAFP+PT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQE-AMLIEDENAKVKGTGSGFIWDK 120
           + QEEDRIV+LFQETSPSVVYIKDLE+AKNPQN S+E  MLIED+N KVKGTGSGF+WDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 180
           FGHIVTNYHVVSALATDNSG QRCKVNLVDVKGNGIYKEA IVGFDPEYDLAVLKVELEG
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEANIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]
BLAST of Lsi02G009020 vs. ExPASy TrEMBL
Match: A0A1S3B9W5 (protease Do-like 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487774 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.4e-156
Identity = 283/305 (92.79%), Postives = 293/305 (96.07%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MALG LG+ PLPI APPNSSENPLPFTSRRAI+F+PTALMASLLAFP+PT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQE-AMLIEDENAKVKGTGSGFIWDK 120
           + QEEDRIV+LFQETSPSVVYIKDLE+AKNPQN S+E  MLIED+N KVKGTGSGF+WDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 180
           FGHIVTNYHVVSALATDNSG QRCKVNLVDVKGNGIYKEA IVGFDPEYDLAVLKVELEG
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEANIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]
BLAST of Lsi02G009020 vs. ExPASy TrEMBL
Match: A0A0A0LV65 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025870 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.4e-154
Identity = 276/304 (90.79%), Postives = 289/304 (95.07%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MALG LG+ PLP  APPNSS+NPLPFTSRRAI+F+P AL+ SLLAFP+PT+AALPQLQD 
Sbjct: 1   MALGLLGIPPLPFPAPPNSSQNPLPFTSRRAILFSPAALLPSLLAFPLPTHAALPQLQDH 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKF 120
           + QEEDR V+LFQETSPSVVYI DLE+ KNPQ PSQ+ MLIED+N KVKGTGSGF+WDKF
Sbjct: 61  LLQEEDRTVSLFQETSPSVVYINDLELPKNPQAPSQQPMLIEDDNLKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH 180
           GHIVTNYHVVSALATDNSG QRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH
Sbjct: 121 GHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH 180

Query: 181 ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA
Sbjct: 181 ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240

Query: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300
           AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY
Sbjct: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300

Query: 301 SERF 305
           SERF
Sbjct: 301 SERF 304

[Close]
BLAST of Lsi02G009020 vs. ExPASy TrEMBL
Match: A0A6J1KMS0 (protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495580 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.1e-154
Identity = 281/305 (92.13%), Postives = 294/305 (96.39%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MALGSLG+R LP+S+PPNSSE  LPF+SRRAIVFAPT LMASLLAFPVP++AAL QLQD+
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIE-DENAKVKGTGSGFIWDK 120
           VPQEEDRIV LFQETSPSVV IK+LEIAK PQNPS+EAMLIE DENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGI ++AKIVGFDPEYDLAVLKVEL+G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180

Query: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSR+LRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]
BLAST of Lsi02G009020 vs. NCBI nr
Match: XP_038893976.1 (protease Do-like 5, chloroplastic [Benincasa hispida])

HSP 1 Score: 575.1 bits (1481), Expect = 3.6e-160
Identity = 289/304 (95.07%), Postives = 296/304 (97.37%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MALGSLG+R LPIS+PPNS+ENPLP TSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD
Sbjct: 1   MALGSLGIRLLPISSPPNSAENPLPITSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKF 120
           +PQEEDRIVALFQETSPSVVYIKDLE+AKNPQNPS      EDENAKVKGTGSGF+WDKF
Sbjct: 61  IPQEEDRIVALFQETSPSVVYIKDLEVAKNPQNPSG-----EDENAKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH 180
           GHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH 180

Query: 181 ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA
Sbjct: 181 ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240

Query: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300
           AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV+RTVPYLIVYGTPY
Sbjct: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVIRTVPYLIVYGTPY 299

Query: 301 SERF 305
           SERF
Sbjct: 301 SERF 299

[Close]
BLAST of Lsi02G009020 vs. NCBI nr
Match: XP_022927238.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 564.7 bits (1454), Expect = 4.9e-157
Identity = 286/305 (93.77%), Postives = 297/305 (97.38%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MALGSLG+R LP+S+PPNSSE  LPFTSRRAIVFAPTALMASLLAFPVP++AALPQLQD+
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIE-DENAKVKGTGSGFIWDK 120
           VPQEEDRIV LFQETSPSVVYIK+LEIAK PQNPS+EAMLIE DENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGI ++AKIVGFDPEYDLAVLKVELEG
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSR+LRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]
BLAST of Lsi02G009020 vs. NCBI nr
Match: XP_008444456.1 (PREDICTED: protease Do-like 5, chloroplastic [Cucumis melo] >KAA0053993.1 protease Do-like 5 [Cucumis melo var. makuwa] >TYK20678.1 protease Do-like 5 [Cucumis melo var. makuwa])

HSP 1 Score: 560.5 bits (1443), Expect = 9.2e-156
Identity = 283/305 (92.79%), Postives = 293/305 (96.07%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MALG LG+ PLPI APPNSSENPLPFTSRRAI+F+PTALMASLLAFP+PT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQE-AMLIEDENAKVKGTGSGFIWDK 120
           + QEEDRIV+LFQETSPSVVYIKDLE+AKNPQN S+E  MLIED+N KVKGTGSGF+WDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 180
           FGHIVTNYHVVSALATDNSG QRCKVNLVDVKGNGIYKEA IVGFDPEYDLAVLKVELEG
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEANIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]
BLAST of Lsi02G009020 vs. NCBI nr
Match: XP_023520040.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 556.6 bits (1433), Expect = 1.3e-154
Identity = 281/305 (92.13%), Postives = 295/305 (96.72%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MA GSLG+R LP+S+PPNSSE  LPFTSRRAIVFAPTALMASLLAFPVP++AALPQLQD+
Sbjct: 1   MASGSLGIRVLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIE-DENAKVKGTGSGFIWDK 120
           VPQEEDRIV LFQETSPSVV IK+LEIAK PQNPS+EAMLIE DENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNL+DVKGNGI ++AK+VGFDPEYDLAVLKVEL+G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLLDVKGNGISRDAKVVGFDPEYDLAVLKVELQG 180

Query: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSR+LRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 305
           YSERF
Sbjct: 301 YSERF 305

[Close]
BLAST of Lsi02G009020 vs. NCBI nr
Match: XP_004137559.1 (protease Do-like 5, chloroplastic [Cucumis sativus] >KGN63906.1 hypothetical protein Csa_013941 [Cucumis sativus])

HSP 1 Score: 555.4 bits (1430), Expect = 2.9e-154
Identity = 276/304 (90.79%), Postives = 289/304 (95.07%), Query Frame = 0 

Query: 1   MALGSLGLRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60
           MALG LG+ PLP  APPNSS+NPLPFTSRRAI+F+P AL+ SLLAFP+PT+AALPQLQD 
Sbjct: 1   MALGLLGIPPLPFPAPPNSSQNPLPFTSRRAILFSPAALLPSLLAFPLPTHAALPQLQDH 60

Query: 61  VPQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKF 120
           + QEEDR V+LFQETSPSVVYI DLE+ KNPQ PSQ+ MLIED+N KVKGTGSGF+WDKF
Sbjct: 61  LLQEEDRTVSLFQETSPSVVYINDLELPKNPQAPSQQPMLIEDDNLKVKGTGSGFVWDKF 120

Query: 121 GHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH 180
           GHIVTNYHVVSALATDNSG QRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH
Sbjct: 121 GHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGH 180

Query: 181 ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240
           ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA
Sbjct: 181 ELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDA 240

Query: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300
           AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY
Sbjct: 241 AISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPY 300

Query: 301 SERF 305
           SERF
Sbjct: 301 SERF 304

[Close]
BLAST of Lsi02G009020 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 364.4 bits (934), Expect = 9.0e-101
Identity = 186/285 (65.26%), Postives = 225/285 (78.95%), Query Frame = 0 

Query: 29  RRAIVFAPT-ALMASLLA-----FPVPTYAALPQL---QDDVPQEEDRIVALFQETSPSV 88
           RR ++F  + AL +SLL       P+ +  AL Q    ++++ +EE+R V LFQ+TSPSV
Sbjct: 43  RRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNVNLFQKTSPSV 102

Query: 89  VYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKFGHIVTNYHVVSALATDNSG 148
           VYI+ +E+ K     S   +L ++EN K++GTGSGF+WDK GHIVTNYHV++ LATD  G
Sbjct: 103 VYIEAIELPKT----SSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHVIAKLATDQFG 162

Query: 149 LQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGHELKPIVFGTSRNLRVGQSC 208
           LQRCKV+LVD KG    KE KIVG DP+ DLAVLK+E EG EL P+V GTS +LRVGQSC
Sbjct: 163 LQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLGTSNDLRVGQSC 222

Query: 209 YAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVI 268
           +AIGNP+GYE TLT GV+SGLGREIPSPNG++I  AIQTDA I++GNSGGPL+DSYGH I
Sbjct: 223 FAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGNSGGPLLDSYGHTI 282

Query: 269 GVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305
           GVNTATFTRKG+GMSSGVNFAIPIDTVVRTVPYLIVYGT Y +RF
Sbjct: 283 GVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYRDRF 323

[Close]
BLAST of Lsi02G009020 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 194.1 bits (492), Expect = 1.6e-49
Identity = 125/291 (42.96%), Postives = 172/291 (59.11%), Query Frame = 0 

Query: 18  NSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQD----------DVPQEEDR 77
           +  ++ L FT   A+   P  L+ + +A     +AA P ++            +  +E  
Sbjct: 63  DDDDDTLHFTPFSAV--KPFFLLCTSVALSFSLFAASPAVESASAFVVSTPKKLQTDELA 122

Query: 78  IVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKFGHIVTNY 137
            V LFQE +PSVVYI +L +        Q+A  + D     +G+GSGF+WDK GHIVTNY
Sbjct: 123 TVRLFQENTPSVVYITNLAV-------RQDAFTL-DVLEVPQGSGSGFVWDKQGHIVTNY 182

Query: 138 HVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEGHELKPIVF 197
           HV+        G    +V L D        +AK+VGFD + D+AVL+++   ++L+PI  
Sbjct: 183 HVI-------RGASDLRVTLAD----QTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPV 242

Query: 198 GTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPS-PNGRAIRGAIQTDAAISAGN 257
           G S +L VGQ  +AIGNPFG + TLT GVISGL REI S   GR I+  IQTDAAI+ GN
Sbjct: 243 GVSADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGN 302

Query: 258 SGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYG 298
           SGGPL+DS G +IG+NTA ++   +G SSGV F+IP+DTV   V  L+ +G
Sbjct: 303 SGGPLLDSSGTLIGINTAIYS--PSGASSGVGFSIPVDTVGGIVDQLVRFG 330

[Close]
BLAST of Lsi02G009020 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 190.7 bits (483), Expect = 1.8e-48
Identity = 131/297 (44.11%), Postives = 176/297 (59.26%), Query Frame = 0 

Query: 8   LRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDDV------ 67
           L  L + + P+++   L  +    + F P+  + S LA   P+ A +  +   V      
Sbjct: 54  LHELAVKSVPSTTRRILLTSLFMNLCFNPSRYL-SALALGDPSVATVEDVSPTVFPAGPL 113

Query: 68  PQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKFG 127
              E RIV LF++ + SVV I D+ +   PQ      + I +      G GSG +WD  G
Sbjct: 114 FPTEGRIVQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQG 173

Query: 128 HIVTNYHVV-SALATDNS-GLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 187
           +IVTNYHV+ +AL+ + S G    +VN++   G     E K+VG D   DLAVLKV+   
Sbjct: 174 YIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPE 233

Query: 188 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 247
             LKPI  G S +L+VGQ C AIGNPFG++ TLT GVISGL R+I S  G  I G IQTD
Sbjct: 234 TLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTD 293

Query: 248 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVY 297
           AAI+ GNSGGPL+DS G++IG+NTA FT+  TG S+GV FAIP  TV++ VP LI +
Sbjct: 294 AAINPGNSGGPLLDSKGNLIGINTAIFTQ--TGTSAGVGFAIPSSTVLKIVPQLIQF 339

[Close]
BLAST of Lsi02G009020 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 168.3 bits (425), Expect = 9.4e-42
Identity = 122/297 (41.08%), Postives = 166/297 (55.89%), Query Frame = 0 

Query: 8   LRPLPISAPPNSSENPLPFTSRRAIVFAPTALMASLLAFPVPTYAALPQLQDDV------ 67
           L  L + + P+++   L  +    + F P+  + S LA   P+ A +  +   V      
Sbjct: 54  LHELAVKSVPSTTRRILLTSLFMNLCFNPSRYL-SALALGDPSVATVEDVSPTVFPAGPL 113

Query: 68  PQEEDRIVALFQETSPSVVYIKDLEIAKNPQNPSQEAMLIEDENAKVKGTGSGFIWDKFG 127
              E RIV LF++ + SVV I D+ +   PQ      + I +      G GSG +WD  G
Sbjct: 114 FPTEGRIVQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQG 173

Query: 128 HIVTNYHVV-SALATDNS-GLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 187
           +IVTNYHV+ +AL+ + S G    +VN++   G     E K+VG D   DLAVLKV+   
Sbjct: 174 YIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPE 233

Query: 188 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 247
             LKPI  G S +L+VGQ C AIGNPFG++ TLT GVISGL R+I S  G  I G IQTD
Sbjct: 234 TLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTD 293

Query: 248 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVY 297
           AAI+ GNSGGPL+DS G++IG+NTA FT+                TV++ VP LI +
Sbjct: 294 AAINPGNSGGPLLDSKGNLIGINTAIFTQ----------------TVLKIVPQLIQF 325

[Close]
BLAST of Lsi02G009020 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-17
Identity = 65/186 (34.95%), Postives = 96/186 (51.61%), Query Frame = 0 

Query: 109 KGTGSGFIWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDVK-GNGIYKEAKIVGFDPE 168
           K  GSG I D  G I+T  HVV     D   ++      VDV   +G   E  +V  D +
Sbjct: 146 KSIGSGTIIDADGTILTCAHVV----VDFQNIRHSSKGRVDVTLQDGRTFEGVVVNADLQ 205

Query: 169 YDLAVLKVELEGHELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSP 228
            D+A++K++ +   L     G S  LR G    A+G P   + T+TAG++S + R+    
Sbjct: 206 SDIALVKIKSK-TPLPTAKLGFSSKLRPGDWVIAVGCPLSLQNTVTAGIVSCVDRKSSDL 265

Query: 229 N-GRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV 288
             G   R  +QTD +I+AGNSGGPLV+  G VIGVN           + G+ F++PID+V
Sbjct: 266 GLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVNIMKVL-----AADGLGFSVPIDSV 321

Query: 289 VRTVPY 293
            + + +
Sbjct: 326 SKIIEH 321

The following BLAST results are available for this feature:
BLAST of Lsi02G009020 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SEL71.3e-9965.26Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 ... [more]
O226092.3e-4842.96Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ... [more]
Q9LU102.5e-4744.11Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ... [more]
Q2SL366.2e-3044.75Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain... [more]
Q4KGQ44.4e-2835.83Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fluorescens (s... [more]
BLAST of Lsi02G009020 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EKF82.4e-15793.77protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A5D3DAW04.4e-15692.79Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G0... [more]
A0A1S3B9W54.4e-15692.79protease Do-like 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487774 PE=3 S... [more]
A0A0A0LV651.4e-15490.79Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025870 PE=3 SV=1[more]
A0A6J1KMS07.1e-15492.13protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
BLAST of Lsi02G009020 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038893976.13.6e-16095.07protease Do-like 5, chloroplastic [Benincasa hispida][more]
XP_022927238.14.9e-15793.77protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata][more]
XP_008444456.19.2e-15692.79PREDICTED: protease Do-like 5, chloroplastic [Cucumis melo] >KAA0053993.1 protea... [more]
XP_023520040.11.3e-15492.13protease Do-like 5, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_004137559.12.9e-15490.79protease Do-like 5, chloroplastic [Cucumis sativus] >KGN63906.1 hypothetical pro... [more]
BLAST of Lsi02G009020 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G18370.19.0e-10165.26DEGP protease 5 [more]
AT3G27925.11.6e-4942.96DegP protease 1 [more]
AT5G39830.11.8e-4844.11Trypsin family protein with PDZ domain [more]
AT5G39830.29.4e-4241.08Trypsin family protein with PDZ domain [more]
AT5G27660.11.2e-1734.95Trypsin family protein with PDZ domain [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001940Peptidase S1CPRINTSPR00834PROTEASES2Ccoord: 232..249
score: 62.74
coord: 195..219
score: 48.88
coord: 254..271
score: 37.67
coord: 121..133
score: 50.47
IPR043504Peptidase S1, PA clan, chymotrypsin-like foldGENE3D2.40.10.10coord: 59..185
e-value: 6.5E-32
score: 111.7
IPR043504Peptidase S1, PA clan, chymotrypsin-like foldGENE3D2.40.10.10coord: 186..298
e-value: 5.0E-40
score: 138.3
NoneNo IPR availablePFAMPF13365Trypsin_2coord: 112..261
e-value: 1.2E-33
score: 117.2
NoneNo IPR availablePANTHERPTHR43343PEPTIDASE S12coord: 18..304
NoneNo IPR availablePANTHERPTHR43343:SF6PROTEASE DO-LIKE 5, CHLOROPLASTIC ISOFORM X1coord: 18..304
IPR009003Peptidase S1, PA clanSUPERFAMILY50494Trypsin-like serine proteasescoord: 70..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G009020.1Lsi02G009020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0004252 serine-type endopeptidase activity