Lsi02G008290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G008290
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionWRKY protein
Locationchr02: 7734065 .. 7735847 (+)
RNA-Seq ExpressionLsi02G008290
SyntenyLsi02G008290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATCTCCCCATTAATATTTTTGTCAACTCCATACCCCATTTTTTTAATTTTAAAAAAAAAAAAACCCCACATTAAATTGCCCCTTTATTCTTCTTCTTCTTCTTCTTCTTCTTCTTGGTTCTCTCTTCTTCAAAACACTCTGATACAAACTTTTCCGACAGCCATTAACCTTAAAAAACCAGCGCCCATCTGTGGCGGCCGGCCATAAATAATATGGATGATGTTACCTTCAACAATAATAACCACCAATTTTATTCTGCTGATCTATTAGAAGATTCCGACGAACTAAAACCCGCCCCTGATTCTCCTTATCCTAATTACGCCGCCACCAAGAAGGGCAGGTCAGTCTTCTATTTTTCATTAAATTAAACCCAATTCAAATGGGTTTTATTTTATTTTAATTTATGGTGTGTTTATGTAGAAGAGGGATGAAGAAGAAAGTTGTGTCGGTGAAAATTAATGGCGACAGCCCAAGAAATTCCTCTGGTTCTACAACTCCACCTTCTGATTCTTGGGCCTGGAGAAAGTACGGTCAAAAGCCCATCAAAGGCTCCCCTTATCCCAGGTTTGATTTAATTTTGGCGTTTGAAATGATAATTGATGAGCTATATTCGAATTGAGAAATTTTGGTTTAATTTTGCAGGGCGTATTATAGATGTAGTAGTTCAAAGGGATGTCCAGCGAGGAAGCAAGTAGAGAGGAATCGGCTTGATCCAACCATGTTGGTCATTACATATTCGTGTGAGCATAATCATTCCGGTCCGGTTTCAAGAAACAACAATAACAATCACAACCAAACCGCTGCGGTGAAACCCGATTCACCAGAGACGGTTCCGGTTCATCAAGAACCGGAAGTGGAAGAGAAATTTGTGGAGATAGTGGGAGAGGAGTCGTTGATAACAGGGGATGAGTTCAGTTGGTTTGGGGAAATGGAAACGACGTCGTCTACAGTGCTTGAAAGCTCCATTTTTTCAGGGCGAGCATCCAGTGGATTGGTGGATCATTCAATTGCAAGTGACGTGGCAATGCTTTTGCCGATGGGAGATGATGACGTGGACGAATCACTCTTTGCGGATCTCGGCGAGTTGCCGGAGTGTTCTCTTGTGTTCCGGCGCGGCGGCGGTAGGGGATTACCGGTGGATGAACAACCGGCTGCAGCCCAGCGTCGGATCACGCCGTGGTGTGGAACCACCACGTGAGATGAGATGGGCGGCCCTCACTTTGTTTGTATACTTCTAGGAGCATCCTCCGCCATTGATGCCGGGAAAGTTTTCTTTTTTCTTCTTCTTTTTTTTTTCTTTTCTAAACTTCATTTTCTTTCTCTTTATATATTTTTTCGAATATGCATTATAAAAAAAAATAGAAAGAAAGAAAATCTTGTTTCATTTTATTCTAAGGTTAAATTACCATTTTAATCTATCCTTATAGTTTGTGTTGAATTTTAATTTGAGCTATATATTGTTTTAAAATTTTCAATTTTAAGCATTCATAAATTTGATTAAATTTCTCAAAATACTATTATTGTTACCAAAACTTAACCACATAGTGACGGATATTGTTTAGATATTACTAAATTGATGTTTAAAGAAAATATGTCACGTTAAGAAGGTGGTATTAGTTTGGATAAAGTAATAGTGTTTTGAGAATTTTCATCAAATATTATAGAGTTTGCCAAATTGAAACTTTTAAAATAGCTAAAATGGTAATTTAACCTTACTAATAAGTATCCATCTTTGTATAACTTGGGTGATATTTGTCTTCATGTTAAATTCTTCTTCTTACG

mRNA sequence

CATCTCCCCATTAATATTTTTGTCAACTCCATACCCCATTTTTTTAATTTTAAAAAAAAAAAAACCCCACATTAAATTGCCCCTTTATTCTTCTTCTTCTTCTTCTTCTTCTTCTTGGTTCTCTCTTCTTCAAAACACTCTGATACAAACTTTTCCGACAGCCATTAACCTTAAAAAACCAGCGCCCATCTGTGGCGGCCGGCCATAAATAATATGGATGATGTTACCTTCAACAATAATAACCACCAATTTTATTCTGCTGATCTATTAGAAGATTCCGACGAACTAAAACCCGCCCCTGATTCTCCTTATCCTAATTACGCCGCCACCAAGAAGGGCAGAAGAGGGATGAAGAAGAAAGTTGTGTCGGTGAAAATTAATGGCGACAGCCCAAGAAATTCCTCTGGTTCTACAACTCCACCTTCTGATTCTTGGGCCTGGAGAAAGTACGGTCAAAAGCCCATCAAAGGCTCCCCTTATCCCAGGGCGTATTATAGATGTAGTAGTTCAAAGGGATGTCCAGCGAGGAAGCAAGTAGAGAGGAATCGGCTTGATCCAACCATGTTGGTCATTACATATTCGTGTGAGCATAATCATTCCGGTCCGGTTTCAAGAAACAACAATAACAATCACAACCAAACCGCTGCGGTGAAACCCGATTCACCAGAGACGGTTCCGGTTCATCAAGAACCGGAAGTGGAAGAGAAATTTGTGGAGATAGTGGGAGAGGAGTCGTTGATAACAGGGGATGAGTTCAGTTGGTTTGGGGAAATGGAAACGACGTCGTCTACAGTGCTTGAAAGCTCCATTTTTTCAGGGCGAGCATCCAGTGGATTGGTGGATCATTCAATTGCAAGTGACGTGGCAATGCTTTTGCCGATGGGAGATGATGACGTGGACGAATCACTCTTTGCGGATCTCGGCGAGTTGCCGGAGTGTTCTCTTGTGTTCCGGCGCGGCGGCGGTAGGGGATTACCGGTGGATGAACAACCGGCTGCAGCCCAGCGTCGGATCACGCCGTGGTGTGGAACCACCACGTGAGATGAGATGGGCGGCCCTCACTTTGTTTGTATACTTCTAGGAGCATCCTCCGCCATTGATGCCGGGAAAGTTTTCTTTTTTCTTCTTCTTTTTTTTTTCTTTTCTAAACTTCATTTTCTTTCTCTTTATATATTTTTTCGAATATGCATTATAAAAAAAAATAGAAAGAAAGAAAATCTTGTTTCATTTTATTCTAAGGTTAAATTACCATTTTAATCTATCCTTATAGTTTGTGTTGAATTTTAATTTGAGCTATATATTGTTTTAAAATTTTCAATTTTAAGCATTCATAAATTTGATTAAATTTCTCAAAATACTATTATTGTTACCAAAACTTAACCACATAGTGACGGATATTGTTTAGATATTACTAAATTGATGTTTAAAGAAAATATGTCACGTTAAGAAGGTGGTATTAGTTTGGATAAAGTAATAGTGTTTTGAGAATTTTCATCAAATATTATAGAGTTTGCCAAATTGAAACTTTTAAAATAGCTAAAATGGTAATTTAACCTTACTAATAAGTATCCATCTTTGTATAACTTGGGTGATATTTGTCTTCATGTTAAATTCTTCTTCTTACG

Coding sequence (CDS)

ATGGATGATGTTACCTTCAACAATAATAACCACCAATTTTATTCTGCTGATCTATTAGAAGATTCCGACGAACTAAAACCCGCCCCTGATTCTCCTTATCCTAATTACGCCGCCACCAAGAAGGGCAGAAGAGGGATGAAGAAGAAAGTTGTGTCGGTGAAAATTAATGGCGACAGCCCAAGAAATTCCTCTGGTTCTACAACTCCACCTTCTGATTCTTGGGCCTGGAGAAAGTACGGTCAAAAGCCCATCAAAGGCTCCCCTTATCCCAGGGCGTATTATAGATGTAGTAGTTCAAAGGGATGTCCAGCGAGGAAGCAAGTAGAGAGGAATCGGCTTGATCCAACCATGTTGGTCATTACATATTCGTGTGAGCATAATCATTCCGGTCCGGTTTCAAGAAACAACAATAACAATCACAACCAAACCGCTGCGGTGAAACCCGATTCACCAGAGACGGTTCCGGTTCATCAAGAACCGGAAGTGGAAGAGAAATTTGTGGAGATAGTGGGAGAGGAGTCGTTGATAACAGGGGATGAGTTCAGTTGGTTTGGGGAAATGGAAACGACGTCGTCTACAGTGCTTGAAAGCTCCATTTTTTCAGGGCGAGCATCCAGTGGATTGGTGGATCATTCAATTGCAAGTGACGTGGCAATGCTTTTGCCGATGGGAGATGATGACGTGGACGAATCACTCTTTGCGGATCTCGGCGAGTTGCCGGAGTGTTCTCTTGTGTTCCGGCGCGGCGGCGGTAGGGGATTACCGGTGGATGAACAACCGGCTGCAGCCCAGCGTCGGATCACGCCGTGGTGTGGAACCACCACGTGA

Protein sequence

MDDVTFNNNNHQFYSADLLEDSDELKPAPDSPYPNYAATKKGRRGMKKKVVSVKINGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVITYSCEHNHSGPVSRNNNNNHNQTAAVKPDSPETVPVHQEPEVEEKFVEIVGEESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDESLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT
Homology
BLAST of Lsi02G008290 vs. ExPASy Swiss-Prot
Match: Q9LP56 (Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 PE=2 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.3e-54
Identity = 119/212 (56.13%), Postives = 146/212 (68.87%), Query Frame = 0

Query: 40  KKGRRGMKKKVVSVKINGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSS 99
           K+ RR ++K+VV+V +         G TTPPSDSWAWRKYGQKPIKGSPYPR YYRCSS+
Sbjct: 43  KRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPSDSWAWRKYGQKPIKGSPYPRGYYRCSST 102

Query: 100 KGCPARKQVERNRLDPTMLVITYSCEHNHSGPVSRNNNNNHNQTAAVKPD-SPETVPVHQ 159
           KGCPARKQVER+R DPTM++ITY+ EHNH  P++ +  N        KP+  PE  P   
Sbjct: 103 KGCPARKQVERSRDDPTMILITYTSEHNHPWPLTSSTRNGPKPKPEPKPEPEPEVEP--- 162

Query: 160 EPEVEEKFVEIVG---EESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIAS 219
           E E E+    ++G   E +    DEF+WF EMETTSST+LES IFS    + +   S A 
Sbjct: 163 EAEEEDNKFMVLGRGIETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAV---SGAD 222

Query: 220 DVAMLLPMGDDDVDESLFADLGELPECSLVFR 248
           DVA+  PMG++  DESLFADLGELPECS+VFR
Sbjct: 223 DVAVFFPMGEE--DESLFADLGELPECSVVFR 246

BLAST of Lsi02G008290 vs. ExPASy Swiss-Prot
Match: Q93WV5 (Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.2e-42
Identity = 115/286 (40.21%), Postives = 156/286 (54.55%), Query Frame = 0

Query: 2   DDVTFNNNNHQFYSADLLEDSDELKPAPDSPYPNYAATKKGRRGMKKKVVSVKINGDSPR 61
           +D T+N+   +  S    EDS   KP P           K RR ++K+VVSV I      
Sbjct: 14  EDETYNDVVPE--SPSSCEDSKISKPTP-----------KKRRNVEKRVVSVPIADVEGS 73

Query: 62  NSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVIT 121
            S G   PPSDSWAWRKYGQKPIKGSPYPR YYRCSSSKGCPARKQVER+R+DP+ L+IT
Sbjct: 74  KSRGEVYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMIT 133

Query: 122 YSCEHNHSGPVSRNNNNNHNQTAAVKPDSPETVPVHQEPEVEEKFVEIVGEES------- 181
           Y+C+HNH  P S  N  +H++++ V     +T    +E E EE+ + +   E        
Sbjct: 134 YACDHNHPFPSSSANTKSHHRSSVVL----KTAKKEEEYEEEEEELTVTAAEEPPAGLDL 193

Query: 182 ------LITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDV 241
                 L+ G  +S  GE         ++SI S   SS  +D ++    ++       + 
Sbjct: 194 SHVDSPLLLGGCYSEIGEF----GWFYDASISSSSGSSNFLDVTLERGFSV-----GQEE 253

Query: 242 DESLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTT 275
           DESLF DLG+LP+C+ VFRRG    +  +EQ         P+C ++
Sbjct: 254 DESLFGDLGDLPDCASVFRRG---TVATEEQHRRCDFGAIPFCDSS 270

BLAST of Lsi02G008290 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.2e-27
Identity = 65/113 (57.52%), Postives = 75/113 (66.37%), Query Frame = 0

Query: 41  KGRRGMKKKVVSVKINGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSK 100
           K R+   KKVV +         SSG    PSD WAWRKYGQKPIKGSPYPR YYRCSSSK
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 101 GCPARKQVERNRLDPTMLVITYSCEHNHSGPVSRN---NNNNHNQTAAVKPDS 151
           GC ARKQVER+R DP MLVITY+ EHNH  P  RN    +   + ++++ P S
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLNPSS 297

BLAST of Lsi02G008290 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 4.4e-27
Identity = 88/224 (39.29%), Postives = 108/224 (48.21%), Query Frame = 0

Query: 41  KGRRGMKKKVVSVKINGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSK 100
           K R+   KKVV +         SSG    PSD WAWRKYGQKPIKGSP+PR YYRCSSSK
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 101 GCPARKQVERNRLDPTMLVITYSCEHNHSGPVSRN-----NNNNHNQTAAVKPDSPETVP 160
           GC ARKQVER+R DP MLVITY+ EHNH  P+ RN       ++ + ++   P  P T  
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSSTSSSSNPNPSKPSTAN 307

Query: 161 VHQEPEVEEKFVEIVGEESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGL----VDH 220
           V+            +G ++ I       +    TT    L SS         +    VD 
Sbjct: 308 VNSSS---------IGSQNTI-------YLPSSTTPPPTLSSSAIKDERGDDMELENVDD 367

Query: 221 SIASDVAMLLPMGDD--DVDESLFADLGELPECSLVFRRGGGRG 254
              + +A   P   D     +  FADL EL   SL      G G
Sbjct: 368 DDDNQIAPYRPELHDHQHQPDDFFADLEELEGDSLSMLLSHGCG 394

BLAST of Lsi02G008290 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.7e-23
Identity = 66/140 (47.14%), Postives = 83/140 (59.29%), Query Frame = 0

Query: 71  SDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVITYSCEHNHSG 130
           SD WAWRKYGQKPIKGSPYPR YYRCS+SKGC ARKQVERNR DP M ++TY+ EHNH  
Sbjct: 128 SDVWAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPA 187

Query: 131 PVSRNN------NNNHNQTAAVKPDSPETVPVHQEPEV--EEKFVEIVGEESLITGDEFS 190
           P  RN+          +Q  +  P +  T+  +    V   ++FV  V E+ L  GD   
Sbjct: 188 PTHRNSLAGSTRQKPSDQQTSKSPTT--TIATYSSSPVTSADEFVLPV-EDHLAVGD-LD 247

Query: 191 WFGEMETTSSTVLESSIFSG 203
              ++ + S TV+    F G
Sbjct: 248 GEEDLLSLSDTVVSDDFFDG 263

BLAST of Lsi02G008290 vs. ExPASy TrEMBL
Match: A0A5D3BED3 (Putative WRKY transcription factor 65 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold34124G00010 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 7.9e-136
Identity = 250/283 (88.34%), Postives = 260/283 (91.87%), Query Frame = 0

Query: 1   MDDVTFN-NNNHQFYSADLLEDSDELKPAPDSPYPN----YAATKKGRRGMKKKVVSVKI 60
           MDDV+FN NN+HQFYS+D  EDSDELKP PDSP P+     AATKKGRRGMKKK++SVKI
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPAPSSTIAAAATKKGRRGMKKKIISVKI 60

Query: 61  NGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120
           NGDSPRNSSGS TPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP
Sbjct: 61  NGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120

Query: 121 TMLVITYSCEHNHSGPVSRNNNNNHNQT---AAVKPDSPETVPVHQEPEVEEKFVEIVGE 180
           T LVITYSCEHNHSGPVSRNNNNN+NQ      +KP SPETV VHQEPEVEEKFVEI GE
Sbjct: 121 TTLVITYSCEHNHSGPVSRNNNNNNNQNNQIVLMKPGSPETVAVHQEPEVEEKFVEIGGE 180

Query: 181 ESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDESL 240
           ESLIT DEFSWFGEMETTSSTVLESSIFSGRAS+GLVDHSI+SDVAML PMGDDDVDESL
Sbjct: 181 ESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDESL 240

Query: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT
Sbjct: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 283

BLAST of Lsi02G008290 vs. ExPASy TrEMBL
Match: A0A1S3B8W7 (probable WRKY transcription factor 65 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487065 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 7.9e-136
Identity = 250/283 (88.34%), Postives = 260/283 (91.87%), Query Frame = 0

Query: 1   MDDVTFN-NNNHQFYSADLLEDSDELKPAPDSPYPN----YAATKKGRRGMKKKVVSVKI 60
           MDDV+FN NN+HQFYS+D  EDSDELKP PDSP P+     AATKKGRRGMKKK++SVKI
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPAPSSTIAAAATKKGRRGMKKKIISVKI 60

Query: 61  NGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120
           NGDSPRNSSGS TPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP
Sbjct: 61  NGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120

Query: 121 TMLVITYSCEHNHSGPVSRNNNNNHNQT---AAVKPDSPETVPVHQEPEVEEKFVEIVGE 180
           T LVITYSCEHNHSGPVSRNNNNN+NQ      +KP SPETV VHQEPEVEEKFVEI GE
Sbjct: 121 TTLVITYSCEHNHSGPVSRNNNNNNNQNNQIVLMKPGSPETVAVHQEPEVEEKFVEIGGE 180

Query: 181 ESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDESL 240
           ESLIT DEFSWFGEMETTSSTVLESSIFSGRAS+GLVDHSI+SDVAML PMGDDDVDESL
Sbjct: 181 ESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDESL 240

Query: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT
Sbjct: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 283

BLAST of Lsi02G008290 vs. ExPASy TrEMBL
Match: E7CEW0 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY6 PE=2 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.4e-134
Identity = 250/285 (87.72%), Postives = 258/285 (90.53%), Query Frame = 0

Query: 1   MDDVTFN-NNNHQFYSADLLEDSDELKPAPDSPYPN-----YAATKKGRRGMKKKVVSVK 60
           MDDV+FN NN+HQFYS+D  EDSDELKP PDSP P       A TKKGRRGMKKK+VSVK
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPPPTSTIAAAATTKKGRRGMKKKIVSVK 60

Query: 61  INGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLD 120
           INGDSPRNSSGS TPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLD
Sbjct: 61  INGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLD 120

Query: 121 PTMLVITYSCEHNHSGPVSRNNNNNHNQT---AAVKPDSPETVPVHQEPEVEEKFVEI-V 180
           PT LVITYSCEHNHSGPVSRNNNNN+NQ      +KP SPETV VHQEPEVEEKFVEI  
Sbjct: 121 PTTLVITYSCEHNHSGPVSRNNNNNNNQNNQIVVMKPGSPETVAVHQEPEVEEKFVEIGG 180

Query: 181 GEESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDE 240
           GEESLIT DEFSWFGEMETTSSTVLESSIFSGRAS+GLVDHSI+SDVAML PMGDDDVDE
Sbjct: 181 GEESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDE 240

Query: 241 SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT
Sbjct: 241 SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 285

BLAST of Lsi02G008290 vs. ExPASy TrEMBL
Match: A0A1S3B8Z0 (probable WRKY transcription factor 65 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487065 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.4e-134
Identity = 249/283 (87.99%), Postives = 259/283 (91.52%), Query Frame = 0

Query: 1   MDDVTFN-NNNHQFYSADLLEDSDELKPAPDSPYPN----YAATKKGRRGMKKKVVSVKI 60
           MDDV+FN NN+HQFYS+D  EDSDELKP PDSP P+     AATKKG RGMKKK++SVKI
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPAPSSTIAAAATKKG-RGMKKKIISVKI 60

Query: 61  NGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120
           NGDSPRNSSGS TPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP
Sbjct: 61  NGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120

Query: 121 TMLVITYSCEHNHSGPVSRNNNNNHNQT---AAVKPDSPETVPVHQEPEVEEKFVEIVGE 180
           T LVITYSCEHNHSGPVSRNNNNN+NQ      +KP SPETV VHQEPEVEEKFVEI GE
Sbjct: 121 TTLVITYSCEHNHSGPVSRNNNNNNNQNNQIVLMKPGSPETVAVHQEPEVEEKFVEIGGE 180

Query: 181 ESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDESL 240
           ESLIT DEFSWFGEMETTSSTVLESSIFSGRAS+GLVDHSI+SDVAML PMGDDDVDESL
Sbjct: 181 ESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDESL 240

Query: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT
Sbjct: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 282

BLAST of Lsi02G008290 vs. ExPASy TrEMBL
Match: A0A6J1EGX5 (probable WRKY transcription factor 65 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434096 PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 7.1e-121
Identity = 231/282 (81.91%), Postives = 242/282 (85.82%), Query Frame = 0

Query: 1   MDDVTFNNNNHQFYSADLLEDSDELKPAPDSP-------YPNYAATKKGRRGMKKKVVSV 60
           MDDVTF NNNHQFYS DLLEDSDE K APDSP        PN  A KKGRRGMKKKVVSV
Sbjct: 1   MDDVTF-NNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPLPPNSTAAKKGRRGMKKKVVSV 60

Query: 61  KINGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRL 120
           +INGD PRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRL
Sbjct: 61  RINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRL 120

Query: 121 DPTMLVITYSCEHNHSGPVSRNNNNNHNQTAAVKPDSPETVPVHQEPEVEEKFVEIVGEE 180
           DPTML+ITYSCEHNHSGPVSR  NNNH+Q AAVKP SPETVPVH EPE EEKFVE +   
Sbjct: 121 DPTMLLITYSCEHNHSGPVSR--NNNHHQAAAVKPTSPETVPVHPEPEAEEKFVESL--- 180

Query: 181 SLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDESLF 240
                D+FSWFGEMETTSSTVLESSIFSGR+SSG+ D SI+SDVAM  PMGDDDVD+SLF
Sbjct: 181 ----EDQFSWFGEMETTSSTVLESSIFSGRSSSGVGD-SISSDVAMPFPMGDDDVDDSLF 240

Query: 241 ADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           ADLGELPECS+VFR GGG  LPVDEQPA +QRRITPWCG TT
Sbjct: 241 ADLGELPECSVVFRHGGGPELPVDEQPATSQRRITPWCGATT 271

BLAST of Lsi02G008290 vs. NCBI nr
Match: XP_038895519.1 (probable WRKY transcription factor 65 [Benincasa hispida])

HSP 1 Score: 497.3 bits (1279), Expect = 8.6e-137
Identity = 255/284 (89.79%), Postives = 260/284 (91.55%), Query Frame = 0

Query: 1   MDDVTF-NNNNHQFYSADLLEDSDELKPAPDSPYPN---YAATKKGRRGMKKKVVSVKIN 60
           MDDV+F NNNNHQFYSADL EDSDELKP PDSP  N     ATKKGRRGMKKKVVSVKIN
Sbjct: 1   MDDVSFNNNNNHQFYSADLFEDSDELKPTPDSPPRNSGAATATKKGRRGMKKKVVSVKIN 60

Query: 61  GDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPT 120
            DSPRNSSGS  PPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVE+NRLDPT
Sbjct: 61  SDSPRNSSGSAPPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVEKNRLDPT 120

Query: 121 MLVITYSCEHNHSGPVSR---NNNNNHNQT--AAVKPDSPETVPVHQEPEVEEKFVEIVG 180
            LVITYSCEHNHSGPVSR   NNNNNHNQT  AA+KP SPETVPVHQEPEVEEKFVEIVG
Sbjct: 121 TLVITYSCEHNHSGPVSRNNNNNNNNHNQTAAAALKPGSPETVPVHQEPEVEEKFVEIVG 180

Query: 181 EESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDES 240
           EES ITGDEFSWFGEMETTSSTVLESSIFSGR SSGLVDHSI+SDVAML PMGDDDVDES
Sbjct: 181 EESFITGDEFSWFGEMETTSSTVLESSIFSGRGSSGLVDHSISSDVAMLFPMGDDDVDES 240

Query: 241 LFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           LFADLGELPECSLVFRRGGGRGLP+DEQPAA QRRITPWCGTTT
Sbjct: 241 LFADLGELPECSLVFRRGGGRGLPLDEQPAATQRRITPWCGTTT 284

BLAST of Lsi02G008290 vs. NCBI nr
Match: XP_008443489.1 (PREDICTED: probable WRKY transcription factor 65 isoform X1 [Cucumis melo] >KAA0057059.1 putative WRKY transcription factor 65 isoform X1 [Cucumis melo var. makuwa] >TYJ98122.1 putative WRKY transcription factor 65 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 493.0 bits (1268), Expect = 1.6e-135
Identity = 250/283 (88.34%), Postives = 260/283 (91.87%), Query Frame = 0

Query: 1   MDDVTFN-NNNHQFYSADLLEDSDELKPAPDSPYPN----YAATKKGRRGMKKKVVSVKI 60
           MDDV+FN NN+HQFYS+D  EDSDELKP PDSP P+     AATKKGRRGMKKK++SVKI
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPAPSSTIAAAATKKGRRGMKKKIISVKI 60

Query: 61  NGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120
           NGDSPRNSSGS TPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP
Sbjct: 61  NGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120

Query: 121 TMLVITYSCEHNHSGPVSRNNNNNHNQT---AAVKPDSPETVPVHQEPEVEEKFVEIVGE 180
           T LVITYSCEHNHSGPVSRNNNNN+NQ      +KP SPETV VHQEPEVEEKFVEI GE
Sbjct: 121 TTLVITYSCEHNHSGPVSRNNNNNNNQNNQIVLMKPGSPETVAVHQEPEVEEKFVEIGGE 180

Query: 181 ESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDESL 240
           ESLIT DEFSWFGEMETTSSTVLESSIFSGRAS+GLVDHSI+SDVAML PMGDDDVDESL
Sbjct: 181 ESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDESL 240

Query: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT
Sbjct: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 283

BLAST of Lsi02G008290 vs. NCBI nr
Match: XP_008443496.1 (PREDICTED: probable WRKY transcription factor 65 isoform X2 [Cucumis melo])

HSP 1 Score: 486.5 bits (1251), Expect = 1.5e-133
Identity = 249/283 (87.99%), Postives = 259/283 (91.52%), Query Frame = 0

Query: 1   MDDVTFN-NNNHQFYSADLLEDSDELKPAPDSPYPN----YAATKKGRRGMKKKVVSVKI 60
           MDDV+FN NN+HQFYS+D  EDSDELKP PDSP P+     AATKKG RGMKKK++SVKI
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPAPSSTIAAAATKKG-RGMKKKIISVKI 60

Query: 61  NGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120
           NGDSPRNSSGS TPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP
Sbjct: 61  NGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120

Query: 121 TMLVITYSCEHNHSGPVSRNNNNNHNQT---AAVKPDSPETVPVHQEPEVEEKFVEIVGE 180
           T LVITYSCEHNHSGPVSRNNNNN+NQ      +KP SPETV VHQEPEVEEKFVEI GE
Sbjct: 121 TTLVITYSCEHNHSGPVSRNNNNNNNQNNQIVLMKPGSPETVAVHQEPEVEEKFVEIGGE 180

Query: 181 ESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDESL 240
           ESLIT DEFSWFGEMETTSSTVLESSIFSGRAS+GLVDHSI+SDVAML PMGDDDVDESL
Sbjct: 181 ESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDESL 240

Query: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT
Sbjct: 241 FADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 282

BLAST of Lsi02G008290 vs. NCBI nr
Match: NP_001267700.1 (probable WRKY transcription factor 65-like [Cucumis sativus] >ADU52503.1 WRKY protein [Cucumis sativus] >KGN65607.1 hypothetical protein Csa_019884 [Cucumis sativus])

HSP 1 Score: 486.5 bits (1251), Expect = 1.5e-133
Identity = 250/285 (87.72%), Postives = 258/285 (90.53%), Query Frame = 0

Query: 1   MDDVTFN-NNNHQFYSADLLEDSDELKPAPDSPYPN-----YAATKKGRRGMKKKVVSVK 60
           MDDV+FN NN+HQFYS+D  EDSDELKP PDSP P       A TKKGRRGMKKK+VSVK
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPPPTSTIAAAATTKKGRRGMKKKIVSVK 60

Query: 61  INGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLD 120
           INGDSPRNSSGS TPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLD
Sbjct: 61  INGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLD 120

Query: 121 PTMLVITYSCEHNHSGPVSRNNNNNHNQT---AAVKPDSPETVPVHQEPEVEEKFVEI-V 180
           PT LVITYSCEHNHSGPVSRNNNNN+NQ      +KP SPETV VHQEPEVEEKFVEI  
Sbjct: 121 PTTLVITYSCEHNHSGPVSRNNNNNNNQNNQIVVMKPGSPETVAVHQEPEVEEKFVEIGG 180

Query: 181 GEESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDE 240
           GEESLIT DEFSWFGEMETTSSTVLESSIFSGRAS+GLVDHSI+SDVAML PMGDDDVDE
Sbjct: 181 GEESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDE 240

Query: 241 SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT
Sbjct: 241 SLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 285

BLAST of Lsi02G008290 vs. NCBI nr
Match: XP_031737179.1 (probable WRKY transcription factor 65-like isoform X1 [Cucumis sativus])

HSP 1 Score: 480.3 bits (1235), Expect = 1.1e-131
Identity = 248/284 (87.32%), Postives = 256/284 (90.14%), Query Frame = 0

Query: 1   MDDVTFN-NNNHQFYSADLLEDSDELKPAPDSPYPN----YAATKKGRRGMKKKVVSVKI 60
           MDDV+FN NN+HQFYS+D  EDSDELKP PDSP P      AAT K  RGMKKK+VSVKI
Sbjct: 1   MDDVSFNHNNHHQFYSSDPFEDSDELKPTPDSPPPTSTIAAAATTKKGRGMKKKIVSVKI 60

Query: 61  NGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120
           NGDSPRNSSGS TPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP
Sbjct: 61  NGDSPRNSSGSATPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDP 120

Query: 121 TMLVITYSCEHNHSGPVSRNNNNNHNQT---AAVKPDSPETVPVHQEPEVEEKFVEI-VG 180
           T LVITYSCEHNHSGPVSRNNNNN+NQ      +KP SPETV VHQEPEVEEKFVEI  G
Sbjct: 121 TTLVITYSCEHNHSGPVSRNNNNNNNQNNQIVVMKPGSPETVAVHQEPEVEEKFVEIGGG 180

Query: 181 EESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDVDES 240
           EESLIT DEFSWFGEMETTSSTVLESSIFSGRAS+GLVDHSI+SDVAML PMGDDDVDES
Sbjct: 181 EESLITADEFSWFGEMETTSSTVLESSIFSGRASTGLVDHSISSDVAMLFPMGDDDVDES 240

Query: 241 LFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 276
           LFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT
Sbjct: 241 LFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTTT 284

BLAST of Lsi02G008290 vs. TAIR 10
Match: AT1G29280.1 (WRKY DNA-binding protein 65 )

HSP 1 Score: 213.0 bits (541), Expect = 3.0e-55
Identity = 119/212 (56.13%), Postives = 146/212 (68.87%), Query Frame = 0

Query: 40  KKGRRGMKKKVVSVKINGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSS 99
           K+ RR ++K+VV+V +         G TTPPSDSWAWRKYGQKPIKGSPYPR YYRCSS+
Sbjct: 43  KRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPSDSWAWRKYGQKPIKGSPYPRGYYRCSST 102

Query: 100 KGCPARKQVERNRLDPTMLVITYSCEHNHSGPVSRNNNNNHNQTAAVKPD-SPETVPVHQ 159
           KGCPARKQVER+R DPTM++ITY+ EHNH  P++ +  N        KP+  PE  P   
Sbjct: 103 KGCPARKQVERSRDDPTMILITYTSEHNHPWPLTSSTRNGPKPKPEPKPEPEPEVEP--- 162

Query: 160 EPEVEEKFVEIVG---EESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIAS 219
           E E E+    ++G   E +    DEF+WF EMETTSST+LES IFS    + +   S A 
Sbjct: 163 EAEEEDNKFMVLGRGIETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAV---SGAD 222

Query: 220 DVAMLLPMGDDDVDESLFADLGELPECSLVFR 248
           DVA+  PMG++  DESLFADLGELPECS+VFR
Sbjct: 223 DVAVFFPMGEE--DESLFADLGELPECSVVFR 246

BLAST of Lsi02G008290 vs. TAIR 10
Match: AT3G58710.1 (WRKY DNA-binding protein 69 )

HSP 1 Score: 176.8 bits (447), Expect = 2.4e-44
Identity = 116/286 (40.56%), Postives = 157/286 (54.90%), Query Frame = 0

Query: 2   DDVTFNNNNHQFYSADLLEDSDELKPAPDSPYPNYAATKKGRRGMKKKVVSVKINGDSPR 61
           +D T+N+   +  S    EDS   KP P          KK RR ++K+VVSV I      
Sbjct: 14  EDETYNDVVPE--SPSSCEDSKISKPTP----------KKSRRNVEKRVVSVPIADVEGS 73

Query: 62  NSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVIT 121
            S G   PPSDSWAWRKYGQKPIKGSPYPR YYRCSSSKGCPARKQVER+R+DP+ L+IT
Sbjct: 74  KSRGEVYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMIT 133

Query: 122 YSCEHNHSGPVSRNNNNNHNQTAAVKPDSPETVPVHQEPEVEEKFVEIVGEES------- 181
           Y+C+HNH  P S  N  +H++++ V     +T    +E E EE+ + +   E        
Sbjct: 134 YACDHNHPFPSSSANTKSHHRSSVVL----KTAKKEEEYEEEEEELTVTAAEEPPAGLDL 193

Query: 182 ------LITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDV 241
                 L+ G  +S  GE         ++SI S   SS  +D ++    ++       + 
Sbjct: 194 SHVDSPLLLGGCYSEIGEF----GWFYDASISSSSGSSNFLDVTLERGFSV-----GQEE 253

Query: 242 DESLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTT 275
           DESLF DLG+LP+C+ VFRRG    +  +EQ         P+C ++
Sbjct: 254 DESLFGDLGDLPDCASVFRRG---TVATEEQHRRCDFGAIPFCDSS 271

BLAST of Lsi02G008290 vs. TAIR 10
Match: AT3G58710.2 (WRKY DNA-binding protein 69 )

HSP 1 Score: 174.1 bits (440), Expect = 1.6e-43
Identity = 115/286 (40.21%), Postives = 156/286 (54.55%), Query Frame = 0

Query: 2   DDVTFNNNNHQFYSADLLEDSDELKPAPDSPYPNYAATKKGRRGMKKKVVSVKINGDSPR 61
           +D T+N+   +  S    EDS   KP P           K RR ++K+VVSV I      
Sbjct: 14  EDETYNDVVPE--SPSSCEDSKISKPTP-----------KKRRNVEKRVVSVPIADVEGS 73

Query: 62  NSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLVIT 121
            S G   PPSDSWAWRKYGQKPIKGSPYPR YYRCSSSKGCPARKQVER+R+DP+ L+IT
Sbjct: 74  KSRGEVYPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMIT 133

Query: 122 YSCEHNHSGPVSRNNNNNHNQTAAVKPDSPETVPVHQEPEVEEKFVEIVGEES------- 181
           Y+C+HNH  P S  N  +H++++ V     +T    +E E EE+ + +   E        
Sbjct: 134 YACDHNHPFPSSSANTKSHHRSSVVL----KTAKKEEEYEEEEEELTVTAAEEPPAGLDL 193

Query: 182 ------LITGDEFSWFGEMETTSSTVLESSIFSGRASSGLVDHSIASDVAMLLPMGDDDV 241
                 L+ G  +S  GE         ++SI S   SS  +D ++    ++       + 
Sbjct: 194 SHVDSPLLLGGCYSEIGEF----GWFYDASISSSSGSSNFLDVTLERGFSV-----GQEE 253

Query: 242 DESLFADLGELPECSLVFRRGGGRGLPVDEQPAAAQRRITPWCGTT 275
           DESLF DLG+LP+C+ VFRRG    +  +EQ         P+C ++
Sbjct: 254 DESLFGDLGDLPDCASVFRRG---TVATEEQHRRCDFGAIPFCDSS 270

BLAST of Lsi02G008290 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 125.2 bits (313), Expect = 8.3e-29
Identity = 65/113 (57.52%), Postives = 75/113 (66.37%), Query Frame = 0

Query: 41  KGRRGMKKKVVSVKINGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSK 100
           K R+   KKVV +         SSG    PSD WAWRKYGQKPIKGSPYPR YYRCSSSK
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 101 GCPARKQVERNRLDPTMLVITYSCEHNHSGPVSRN---NNNNHNQTAAVKPDS 151
           GC ARKQVER+R DP MLVITY+ EHNH  P  RN    +   + ++++ P S
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLNPSS 297

BLAST of Lsi02G008290 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 123.2 bits (308), Expect = 3.2e-28
Identity = 88/224 (39.29%), Postives = 108/224 (48.21%), Query Frame = 0

Query: 41  KGRRGMKKKVVSVKINGDSPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSK 100
           K R+   KKVV +         SSG    PSD WAWRKYGQKPIKGSP+PR YYRCSSSK
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 101 GCPARKQVERNRLDPTMLVITYSCEHNHSGPVSRN-----NNNNHNQTAAVKPDSPETVP 160
           GC ARKQVER+R DP MLVITY+ EHNH  P+ RN       ++ + ++   P  P T  
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSSTSSSSNPNPSKPSTAN 307

Query: 161 VHQEPEVEEKFVEIVGEESLITGDEFSWFGEMETTSSTVLESSIFSGRASSGL----VDH 220
           V+            +G ++ I       +    TT    L SS         +    VD 
Sbjct: 308 VNSSS---------IGSQNTI-------YLPSSTTPPPTLSSSAIKDERGDDMELENVDD 367

Query: 221 SIASDVAMLLPMGDD--DVDESLFADLGELPECSLVFRRGGGRG 254
              + +A   P   D     +  FADL EL   SL      G G
Sbjct: 368 DDDNQIAPYRPELHDHQHQPDDFFADLEELEGDSLSMLLSHGCG 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LP564.3e-5456.13Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 ... [more]
Q93WV52.2e-4240.21Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 ... [more]
O647471.2e-2757.52Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
Q9SA804.4e-2739.29Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ... [more]
O046091.7e-2347.14WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BED37.9e-13688.34Putative WRKY transcription factor 65 isoform X1 OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3B8W77.9e-13688.34probable WRKY transcription factor 65 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
E7CEW07.4e-13487.72WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY6 PE=2 SV=1[more]
A0A1S3B8Z07.4e-13487.99probable WRKY transcription factor 65 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1EGX57.1e-12181.91probable WRKY transcription factor 65 isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
XP_038895519.18.6e-13789.79probable WRKY transcription factor 65 [Benincasa hispida][more]
XP_008443489.11.6e-13588.34PREDICTED: probable WRKY transcription factor 65 isoform X1 [Cucumis melo] >KAA0... [more]
XP_008443496.11.5e-13387.99PREDICTED: probable WRKY transcription factor 65 isoform X2 [Cucumis melo][more]
NP_001267700.11.5e-13387.72probable WRKY transcription factor 65-like [Cucumis sativus] >ADU52503.1 WRKY pr... [more]
XP_031737179.11.1e-13187.32probable WRKY transcription factor 65-like isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G29280.13.0e-5556.13WRKY DNA-binding protein 65 [more]
AT3G58710.12.4e-4440.56WRKY DNA-binding protein 69 [more]
AT3G58710.21.6e-4340.21WRKY DNA-binding protein 69 [more]
AT2G34830.18.3e-2957.52WRKY DNA-binding protein 35 [more]
AT1G30650.13.2e-2839.29WRKY DNA-binding protein 14 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 70..130
e-value: 1.3E-34
score: 131.0
IPR003657WRKY domainPFAMPF03106WRKYcoord: 72..128
e-value: 5.6E-25
score: 87.4
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 71..131
score: 31.42174
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 57..132
e-value: 2.7E-30
score: 106.0
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 67..130
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..80
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..74
NoneNo IPR availablePANTHERPTHR32096:SF19OS01G0750100 PROTEINcoord: 31..273
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 31..273

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G008290.1Lsi02G008290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000976 transcription cis-regulatory region binding
molecular_function GO:0043565 sequence-specific DNA binding