Lsi02G006190 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G006190 vs. ExPASy Swiss-Prot
Match: P43283 (Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.6e-42
Identity = 107/302 (35.43%), Postives = 165/302 (54.64%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      A+++ ++AK +G++R++     GQ G+ TA   A++GL C I+M + D+
Sbjct: 77  LNHRGAHKINNAVAQALLAKRLGKQRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDM 136

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ERQ+ +V  +  L A+V  V    A    A     T     +W+ N E + Y++ +V  P
Sbjct: 137 ERQALNVFRMKLLGAEVRAVHSGTATLKDA-----TSEAIRDWVTNVETTHYILGSVAGP 196

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + RE   +IG+ET++Q   +WGGK PDV++  +G  SNA+ LFHE + ++DVRLI
Sbjct: 197 HPYPMMVREFHKVIGKETRRQAMHKWGGK-PDVLVACVGGGSNAMGLFHEFVEDQDVRLI 256

Query: 181 GVEA-----DSEQ----LTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA     D+++    LT   + +                    +I   L+Y    P+ 
Sbjct: 257 GVEAAGHGVDTDKHAATLTKGQVGVLHGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEH 316

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLK+  + EY  V D EA +A+  V +LE   I P+ + S+A AY  KLCPTLPDG +V
Sbjct: 317 SFLKDIGRAEYDSVTDQEALDAFKRVSRLE--GIIPALETSHALAYLEKLCPTLPDGVRV 370

BLAST of Lsi02G006190 vs. ExPASy Swiss-Prot
Match: P25269 (Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB2 PE=1 SV=2)

HSP 1 Score: 172.2 bits (435), Expect = 8.1e-42
Identity = 106/302 (35.10%), Postives = 163/302 (53.97%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      A+++ ++AK +G+KR++     GQ G+ TA   A++GL C I+M + D+
Sbjct: 163 LNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDM 222

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ERQ+ +V  +  L A+V  V    A    A     T     +W+ N E + Y++ +V  P
Sbjct: 223 ERQALNVFRMRLLGAEVRGVHSGTATLKDA-----TSEAIRDWVTNVETTHYILGSVAGP 282

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + R+  ++IG+ET+KQ  E+WGGK PDV++  +G  SNA+ LFHE + + +VR+I
Sbjct: 283 HPYPMMVRDFHAVIGKETRKQAMEKWGGK-PDVLVACVGGGSNAMGLFHEFVDDTEVRMI 342

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          +  LT   + +                    +I   L+Y    P+ 
Sbjct: 343 GVEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEH 402

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLK+  + EY  V D EA EA+  V +LE   I P+ + S+A A+  KLCPTLPDG +V
Sbjct: 403 SFLKDVGRAEYFSVTDEEALEAFKRVSRLE--GIIPALETSHALAHLEKLCPTLPDGARV 456

BLAST of Lsi02G006190 vs. ExPASy Swiss-Prot
Match: O50046 (Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=16922 GN=TSB PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.1e-41
Identity = 106/302 (35.10%), Postives = 163/302 (53.97%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      A+++ ++AK +G+KR++     GQ G+ TA   A++GL C I+M + D+
Sbjct: 154 LNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCVIYMGAQDM 213

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ERQ+ +V  +  L A+V  V    A    A     T     +W+ N E + Y++ +V  P
Sbjct: 214 ERQALNVFRMRLLGAEVRAVHSGTATLKDA-----TSEAIRDWVTNVESTHYILGSVAGP 273

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + RE  ++IG+ET+KQ  E+WGGK PDV++  +G  SNA+ LFHE + ++DVR+I
Sbjct: 274 HPYPMMVREFHAVIGKETRKQALEKWGGK-PDVLVACVGGGSNAMGLFHEFVDDKDVRMI 333

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          +  LT   + +                    +I   L+Y    P+ 
Sbjct: 334 GVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEH 393

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLK+  + EY    D EA EA+  + +LE   I P+ + S+A A+  KLCPTLP+G KV
Sbjct: 394 SFLKDIGRAEYYCCTDEEALEAFKRLSRLE--GIIPALETSHALAFLEKLCPTLPNGTKV 447

BLAST of Lsi02G006190 vs. ExPASy Swiss-Prot
Match: P14671 (Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.4e-41
Identity = 104/302 (34.44%), Postives = 163/302 (53.97%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      A+++ ++AK +G+KR++     GQ G+ TA   A++GL+C I+M + D+
Sbjct: 158 LNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDM 217

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ERQ+ +V  +  L A+V  V    A    A     T     +W+ N E + Y++ +V  P
Sbjct: 218 ERQALNVFRMRLLGAEVRGVHSGTATLKDA-----TSEAIRDWVTNVETTHYILGSVAGP 277

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + R+  ++IG+ET+KQ  E+WGGK PDV++  +G  SNA+ LFHE +++ +VR+I
Sbjct: 278 HPYPMMVRDFHAVIGKETRKQALEKWGGK-PDVLVACVGGGSNAMGLFHEFVNDTEVRMI 337

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          +  LT   + +                    +I   L+Y    P+ 
Sbjct: 338 GVEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEH 397

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            F K+  + EY  + D EA EA+  V +LE   I P+ + S+A AY  KLCPTL DG +V
Sbjct: 398 SFFKDMGRAEYYSITDEEALEAFKRVSRLE--GIIPALETSHALAYLEKLCPTLSDGTRV 451

BLAST of Lsi02G006190 vs. ExPASy Swiss-Prot
Match: P43284 (Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 GN=TSB2 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.1e-41
Identity = 104/302 (34.44%), Postives = 164/302 (54.30%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      A+++ ++AK +G++R++     GQ G+ TA    ++GL C I+M + D+
Sbjct: 131 LNHTGAHKINNAVAQALLAKRLGKQRIIAETGAGQHGVATATVCRRFGLQCIIYMGAQDM 190

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ERQ+ +V  +  L A+V  V    A    A     T     +W+ N E + Y++ +V  P
Sbjct: 191 ERQALNVFRMRLLGAEVRAVHSGTATLKDA-----TSEAIRDWVTNVETTHYILGSVAGP 250

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + RE   +IG+ET++Q  ++WGGK PDV++  +G  SNA+ LFHE + ++DVRL+
Sbjct: 251 HPYPMMVREFHKVIGKETRRQAMDKWGGK-PDVLVACVGGGSNAMGLFHEFVEDQDVRLV 310

Query: 181 GVEA-----DSEQ----LTNFSIHI--------------------TIMFKLEYQREEPKL 240
           G+EA     D+++    LT   + +                    +I   L+Y    P+ 
Sbjct: 311 GLEAAGHGVDTDKHAATLTKGQVGVLHGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEH 370

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLK+  + EY  V D EA +A+  V +LE   I P+ + S+A AY  KLCPTL DG +V
Sbjct: 371 SFLKDIGRAEYDSVTDQEALDAFKRVSRLE--GIIPALETSHALAYLEKLCPTLADGVRV 424

BLAST of Lsi02G006190 vs. ExPASy TrEMBL
Match: A0A5D3BMJ6 (Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005960 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 3.9e-55
Identity = 143/302 (47.35%), Postives = 182/302 (60.26%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      AI++V+IAK MGRK VV A   GQ G+ TA A AK+ LDCTIFM S DI
Sbjct: 145 LNHCGAHKMNNAIAQVMIAKRMGRKSVVAATGAGQHGVATAAACAKHDLDCTIFMGSEDI 204

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
            +QSS+V LI  L A+V+ V+GN   AS  A          EW+ N E S YL  TVV P
Sbjct: 205 NKQSSNVLLIKLLGAKVKSVEGNFKDASSEA--------IREWVGNLETSYYLTGTVVGP 264

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           +P   + RE QS+IG+ET++Q +E+WG K PDV+L  IGS SNAL LFHE I+E+DVRLI
Sbjct: 265 YPCPAMVREFQSVIGKETRRQAKEKWGAK-PDVLLACIGSGSNALGLFHEFINEKDVRLI 324

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          S  L+   + +                    ++   LEY    P+L
Sbjct: 325 GVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDDEGQILNPHSVGVGLEYPGVGPEL 384

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLKE+ + E++  +D+EA EAY  + KLE   I+PS + S+AFAY HKLCPTLPDG KV
Sbjct: 385 SFLKESGRAEFETASDTEAVEAYKRLAKLE--GIFPSLEASHAFAYLHKLCPTLPDGCKV 435

BLAST of Lsi02G006190 vs. ExPASy TrEMBL
Match: A0A1S3B3A1 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103485497 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 3.9e-55
Identity = 143/302 (47.35%), Postives = 182/302 (60.26%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      AI++V+IAK MGRK VV A   GQ G+ TA A AK+ LDCTIFM S DI
Sbjct: 144 LNHCGAHKMNNAIAQVMIAKRMGRKSVVAATGAGQHGVATAAACAKHDLDCTIFMGSEDI 203

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
            +QSS+V LI  L A+V+ V+GN   AS  A          EW+ N E S YL  TVV P
Sbjct: 204 NKQSSNVLLIKLLGAKVKSVEGNFKDASSEA--------IREWVGNLETSYYLTGTVVGP 263

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           +P   + RE QS+IG+ET++Q +E+WG K PDV+L  IGS SNAL LFHE I+E+DVRLI
Sbjct: 264 YPCPAMVREFQSVIGKETRRQAKEKWGAK-PDVLLACIGSGSNALGLFHEFINEKDVRLI 323

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          S  L+   + +                    ++   LEY    P+L
Sbjct: 324 GVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDDEGQILNPHSVGVGLEYPGVGPEL 383

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLKE+ + E++  +D+EA EAY  + KLE   I+PS + S+AFAY HKLCPTLPDG KV
Sbjct: 384 SFLKESGRAEFETASDTEAVEAYKRLAKLE--GIFPSLEASHAFAYLHKLCPTLPDGCKV 434

BLAST of Lsi02G006190 vs. ExPASy TrEMBL
Match: A0A6J1K0T4 (Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111490065 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 6.7e-55
Identity = 146/302 (48.34%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      AI++V+IAK MGR+RVV A   GQ G+ TA A AK+ L+CTIFM S DI
Sbjct: 147 LNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDI 206

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ++QSS+V LIN L AQV+ V+GN   AS  A          EW+ N E S YLM TVV P
Sbjct: 207 KKQSSNVVLINLLGAQVKSVEGNFKDASSEA--------IREWVGNLEMSYYLMGTVVGP 266

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + RE QS+IG+ET+KQ  E+WGG LPDV++  IGS SNAL LF+E I EEDVRLI
Sbjct: 267 HPCPAMVREFQSVIGKETRKQAMEKWGG-LPDVLVACIGSGSNALGLFNEFIKEEDVRLI 326

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          S  L+   + +                    ++   LEY    P+L
Sbjct: 327 GVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDEEGQILVPHSVGVGLEYPGVGPEL 386

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLKE+ + E+    D EA EAY  V KLE   I+PS + S+AFAY  KLCPTL DG KV
Sbjct: 387 SFLKESGRAEFHTAIDKEAVEAYKRVCKLE--GIFPSLEASHAFAYLDKLCPTLRDGCKV 437

BLAST of Lsi02G006190 vs. ExPASy TrEMBL
Match: A0A0A0LUA1 (Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_1G064170 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.5e-54
Identity = 141/302 (46.69%), Postives = 182/302 (60.26%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      AI++V+IAK MGRK VV A   GQ G+ TA A AK+ LDCTIFM + DI
Sbjct: 144 LNHCGAHKMNNAIAQVMIAKRMGRKSVVAATGAGQHGVATAAACAKHDLDCTIFMGTEDI 203

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ++QSS+V LI  L A+V+ V+GN   AS  A           W+ N E S YL  TVV P
Sbjct: 204 KKQSSNVLLIKMLGAKVKAVEGNFKDASSEA--------IRGWVGNLETSYYLTGTVVGP 263

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + RE QS+IG+ET++Q  E+WG K PDV+L  IGS SNAL LFHE I+E+DVRLI
Sbjct: 264 HPCPAMVREFQSVIGKETRRQAMEKWGAK-PDVLLACIGSGSNALGLFHEFINEKDVRLI 323

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          S  L+   + +                    ++   LEY    P+L
Sbjct: 324 GVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDDEGQILNPHSVGVGLEYPGVGPEL 383

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLK++ + E++  +D+EA EAY L+ KLE   I+P+ + S+AFAY HKLCPTLPDG KV
Sbjct: 384 SFLKDSGRAEFETASDTEAVEAYKLLAKLE--GIFPALEASHAFAYLHKLCPTLPDGCKV 434

BLAST of Lsi02G006190 vs. ExPASy TrEMBL
Match: A0A6J1GSL9 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457067 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 9.6e-54
Identity = 143/302 (47.35%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      AI++V+IAK MGR+RVV A   GQ G+ TA A AK+ L+CTIFM S DI
Sbjct: 148 LNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDI 207

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ++QSS+V LI  L AQV+ V+GN   AS  A          EW+ N ERS YL  TVV P
Sbjct: 208 KKQSSNVVLIKLLGAQVKSVEGNFKDASSEA--------IREWVGNLERSYYLTGTVVGP 267

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + RE QS+IG+ET+KQ  E+WGG LPDV++  IGS SNAL LF+E I+EEDVRLI
Sbjct: 268 HPCPAMVREFQSVIGKETRKQAMEKWGG-LPDVLVACIGSGSNALGLFNEFINEEDVRLI 327

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          S  L+   + +                    ++   LEY    P+L
Sbjct: 328 GVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPEL 387

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLKE+ + E+    D EA EAY  V +LE   I+PS + S+AFAY  +LCPTL DG KV
Sbjct: 388 SFLKESGRAEFHTAIDKEAVEAYKRVCRLE--GIFPSLEASHAFAYLDELCPTLTDGCKV 438

BLAST of Lsi02G006190 vs. NCBI nr
Match: XP_038895944.1 (LOW QUALITY PROTEIN: tryptophan synthase beta chain 1-like [Benincasa hispida])

HSP 1 Score: 238.8 bits (608), Expect = 5.4e-59
Identity = 145/269 (53.90%), Postives = 173/269 (64.31%), Query Frame = 0

Query: 1   MNHFGAISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDIERQSSS 60
           MNHF AI+EV+ AK MGRK VV AIRD QLG+TTAVA AKYGLDCTIFMASYDIE QSSS
Sbjct: 127 MNHFDAIAEVMTAKRMGRKSVVAAIRDAQLGVTTAVACAKYGLDCTIFMASYDIENQSSS 186

Query: 61  VRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEPHPGLDV 120
           V+LIN L AQV+PV+GNL  A   AR EW          N   ++YL  TVV P P    
Sbjct: 187 VQLINCLGAQVKPVEGNLNNALAGARQEW-------MTGNLGTTTYLDGTVVGPRP---- 246

Query: 121 ARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLIGVEADS 180
                       +  D EEWG K PDVV G  G +++   +FH+ I ++DVRLI V    
Sbjct: 247 ------------RPSDPEEWGVK-PDVVFGCTGXSNDLPLVFHDFIQQQDVRLIRVLQPP 306

Query: 181 EQLTNFSIHITIMFKLEYQREEPKLRFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIY 240
             +       +I  ++EY  E  KLR +KET K+E+  VAD+EA EA+DL++KLE   IY
Sbjct: 307 TGMHQILTPHSIGEEVEYTGEGLKLRLVKETGKVEFHTVADTEAVEAHDLLIKLE--GIY 366

Query: 241 PSKDDSYAFAYHHKLCPTLPDGYKVVVVI 270
            SK+ SYAFAY HKLCPTLPD  KVVVVI
Sbjct: 367 LSKEASYAFAYLHKLCPTLPDDCKVVVVI 369

BLAST of Lsi02G006190 vs. NCBI nr
Match: KAA0056875.1 (tryptophan synthase beta chain 1-like [Cucumis melo var. makuwa] >TYJ99378.1 tryptophan synthase beta chain 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 224.9 bits (572), Expect = 8.1e-55
Identity = 143/302 (47.35%), Postives = 182/302 (60.26%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      AI++V+IAK MGRK VV A   GQ G+ TA A AK+ LDCTIFM S DI
Sbjct: 145 LNHCGAHKMNNAIAQVMIAKRMGRKSVVAATGAGQHGVATAAACAKHDLDCTIFMGSEDI 204

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
            +QSS+V LI  L A+V+ V+GN   AS  A          EW+ N E S YL  TVV P
Sbjct: 205 NKQSSNVLLIKLLGAKVKSVEGNFKDASSEA--------IREWVGNLETSYYLTGTVVGP 264

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           +P   + RE QS+IG+ET++Q +E+WG K PDV+L  IGS SNAL LFHE I+E+DVRLI
Sbjct: 265 YPCPAMVREFQSVIGKETRRQAKEKWGAK-PDVLLACIGSGSNALGLFHEFINEKDVRLI 324

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          S  L+   + +                    ++   LEY    P+L
Sbjct: 325 GVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDDEGQILNPHSVGVGLEYPGVGPEL 384

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLKE+ + E++  +D+EA EAY  + KLE   I+PS + S+AFAY HKLCPTLPDG KV
Sbjct: 385 SFLKESGRAEFETASDTEAVEAYKRLAKLE--GIFPSLEASHAFAYLHKLCPTLPDGCKV 435

BLAST of Lsi02G006190 vs. NCBI nr
Match: XP_008441364.1 (PREDICTED: tryptophan synthase beta chain 1-like [Cucumis melo])

HSP 1 Score: 224.9 bits (572), Expect = 8.1e-55
Identity = 143/302 (47.35%), Postives = 182/302 (60.26%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      AI++V+IAK MGRK VV A   GQ G+ TA A AK+ LDCTIFM S DI
Sbjct: 144 LNHCGAHKMNNAIAQVMIAKRMGRKSVVAATGAGQHGVATAAACAKHDLDCTIFMGSEDI 203

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
            +QSS+V LI  L A+V+ V+GN   AS  A          EW+ N E S YL  TVV P
Sbjct: 204 NKQSSNVLLIKLLGAKVKSVEGNFKDASSEA--------IREWVGNLETSYYLTGTVVGP 263

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           +P   + RE QS+IG+ET++Q +E+WG K PDV+L  IGS SNAL LFHE I+E+DVRLI
Sbjct: 264 YPCPAMVREFQSVIGKETRRQAKEKWGAK-PDVLLACIGSGSNALGLFHEFINEKDVRLI 323

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          S  L+   + +                    ++   LEY    P+L
Sbjct: 324 GVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDDEGQILNPHSVGVGLEYPGVGPEL 383

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLKE+ + E++  +D+EA EAY  + KLE   I+PS + S+AFAY HKLCPTLPDG KV
Sbjct: 384 SFLKESGRAEFETASDTEAVEAYKRLAKLE--GIFPSLEASHAFAYLHKLCPTLPDGCKV 434

BLAST of Lsi02G006190 vs. NCBI nr
Match: XP_022994295.1 (tryptophan synthase beta chain 1-like [Cucurbita maxima])

HSP 1 Score: 224.2 bits (570), Expect = 1.4e-54
Identity = 146/302 (48.34%), Postives = 180/302 (59.60%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      AI++V+IAK MGR+RVV A   GQ G+ TA A AK+ L+CTIFM S DI
Sbjct: 147 LNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDI 206

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ++QSS+V LIN L AQV+ V+GN   AS  A          EW+ N E S YLM TVV P
Sbjct: 207 KKQSSNVVLINLLGAQVKSVEGNFKDASSEA--------IREWVGNLEMSYYLMGTVVGP 266

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + RE QS+IG+ET+KQ  E+WGG LPDV++  IGS SNAL LF+E I EEDVRLI
Sbjct: 267 HPCPAMVREFQSVIGKETRKQAMEKWGG-LPDVLVACIGSGSNALGLFNEFIKEEDVRLI 326

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          S  L+   + +                    ++   LEY    P+L
Sbjct: 327 GVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDEEGQILVPHSVGVGLEYPGVGPEL 386

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLKE+ + E+    D EA EAY  V KLE   I+PS + S+AFAY  KLCPTL DG KV
Sbjct: 387 SFLKESGRAEFHTAIDKEAVEAYKRVCKLE--GIFPSLEASHAFAYLDKLCPTLRDGCKV 437

BLAST of Lsi02G006190 vs. NCBI nr
Match: XP_038893791.1 (tryptophan synthase beta chain 1-like [Benincasa hispida])

HSP 1 Score: 223.0 bits (567), Expect = 3.1e-54
Identity = 142/302 (47.02%), Postives = 181/302 (59.93%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      AI++V+IAK MGRK VV A   GQ G+ TA A AK  LDCTIFM S DI
Sbjct: 149 LNHCGAHKMNNAIAQVMIAKRMGRKSVVAATGAGQHGVATAAACAKNDLDCTIFMGSEDI 208

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ++QSS+V LI  L AQV+ V+GN   A+  A          +W+ N E S YL  TVV P
Sbjct: 209 KKQSSNVLLIKLLGAQVKSVEGNFKNATSEA--------IRQWVGNLEMSYYLTGTVVGP 268

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP  D+ RE QS+IG+ET++Q  ++WGGK PDV+L  IGS SNAL LFH+ I E+DVRLI
Sbjct: 269 HPCPDMVREFQSVIGKETRRQAMDKWGGK-PDVLLACIGSGSNALGLFHDFIKEQDVRLI 328

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          S  L+   + +                    ++   LEY    P+L
Sbjct: 329 GVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDDEGQILNPHSVGVGLEYPGVGPEL 388

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLKE+ + E+   +D+EA EAY  + KLE   I+PS + S+AFAY +KLCPTLPDG KV
Sbjct: 389 SFLKESGRAEFHTASDTEAVEAYKRLCKLE--GIFPSLEASHAFAYLNKLCPTLPDGSKV 439

BLAST of Lsi02G006190 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.2e-43
Identity = 114/291 (39.18%), Postives = 158/291 (54.30%), Query Frame = 0

Query: 6   AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDIERQSSSVRLIN 65
           A+++ +I++ +G  RVV A   GQ G+ TA A AK  L+CT+FM + DIE+QS +V  + 
Sbjct: 163 ALAQAMISRRLGCSRVVAATGAGQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMK 222

Query: 66  SLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEPHPGLDVARESQ 125
            L AQV  V+G    AS  A           W+ N   + YL  TVV PHP   + RE Q
Sbjct: 223 LLGAQVISVEGTFKDASSEA--------IRNWVENLYTTYYLSGTVVGPHPCPIIVREFQ 282

Query: 126 SMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLIGVEADSEQLTN 185
           S+IG+ET++Q ++ WGGK PDV++  +GS SNAL LFHE + +EDVRL+GVEA    L +
Sbjct: 283 SVIGKETRRQAKQLWGGK-PDVLVACVGSGSNALGLFHEFVGDEDVRLVGVEAAGLGLDS 342

Query: 186 FSIHITIMF-----------------------------KLEYQREEPKLRFLKETEKIEY 245
                T+ F                              LEY    P++ F+KET + E+
Sbjct: 343 GKHSATLAFGDVGVYHGSMSYLLQDDQGQILKPHSVGVGLEYPGVGPEISFMKETGRAEF 402

Query: 246 QKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKVVV 268
               D EA +A   + +LE   I P+ + S+A A+  KL PTL DG KVVV
Sbjct: 403 YTATDEEAIQACMRLSRLE--GIIPALEASHALAFLDKLVPTLRDGAKVVV 442

BLAST of Lsi02G006190 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 172.2 bits (435), Expect = 5.8e-43
Identity = 106/302 (35.10%), Postives = 163/302 (53.97%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      A+++ ++AK +G+KR++     GQ G+ TA   A++GL C I+M + D+
Sbjct: 163 LNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDM 222

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ERQ+ +V  +  L A+V  V    A    A     T     +W+ N E + Y++ +V  P
Sbjct: 223 ERQALNVFRMRLLGAEVRGVHSGTATLKDA-----TSEAIRDWVTNVETTHYILGSVAGP 282

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + R+  ++IG+ET+KQ  E+WGGK PDV++  +G  SNA+ LFHE + + +VR+I
Sbjct: 283 HPYPMMVRDFHAVIGKETRKQAMEKWGGK-PDVLVACVGGGSNAMGLFHEFVDDTEVRMI 342

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          +  LT   + +                    +I   L+Y    P+ 
Sbjct: 343 GVEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEH 402

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            FLK+  + EY  V D EA EA+  V +LE   I P+ + S+A A+  KLCPTLPDG +V
Sbjct: 403 SFLKDVGRAEYFSVTDEEALEAFKRVSRLE--GIIPALETSHALAHLEKLCPTLPDGARV 456

BLAST of Lsi02G006190 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 170.6 bits (431), Expect = 1.7e-42
Identity = 104/302 (34.44%), Postives = 163/302 (53.97%), Query Frame = 0

Query: 1   MNHFG------AISEVIIAKCMGRKRVVVAIRDGQLGITTAVAYAKYGLDCTIFMASYDI 60
           +NH G      A+++ ++AK +G+KR++     GQ G+ TA   A++GL+C I+M + D+
Sbjct: 158 LNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDM 217

Query: 61  ERQSSSVRLINSLDAQVEPVKGNLAIASQAARVEWTMVTAEEWLRNWERSSYLMRTVVEP 120
           ERQ+ +V  +  L A+V  V    A    A     T     +W+ N E + Y++ +V  P
Sbjct: 218 ERQALNVFRMRLLGAEVRGVHSGTATLKDA-----TSEAIRDWVTNVETTHYILGSVAGP 277

Query: 121 HPGLDVARESQSMIGEETKKQDREEWGGKLPDVVLGYIGSTSNALALFHELIHEEDVRLI 180
           HP   + R+  ++IG+ET+KQ  E+WGGK PDV++  +G  SNA+ LFHE +++ +VR+I
Sbjct: 278 HPYPMMVRDFHAVIGKETRKQALEKWGGK-PDVLVACVGGGSNAMGLFHEFVNDTEVRMI 337

Query: 181 GVEA---------DSEQLTNFSIHI--------------------TIMFKLEYQREEPKL 240
           GVEA          +  LT   + +                    +I   L+Y    P+ 
Sbjct: 338 GVEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEH 397

Query: 241 RFLKETEKIEYQKVADSEAAEAYDLVVKLENKNIYPSKDDSYAFAYHHKLCPTLPDGYKV 268
            F K+  + EY  + D EA EA+  V +LE   I P+ + S+A AY  KLCPTL DG +V
Sbjct: 398 SFFKDMGRAEYYSITDEEALEAFKRVSRLE--GIIPALETSHALAYLEKLCPTLSDGTRV 451