Lsi02G005970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G005970
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Lactoylglutathione lyase
Location	chr02: 5293691 .. 5296718 (+)
RNA-Seq Expression	Lsi02G005970
Synteny	Lsi02G005970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCCAACATTTACTAAACAAATTCATAGTTGTTCAATGCAATAATTAAACTGCACGTTATCAGATTCATCTCCACTGCACTTTTTGGTCTTTGGTCTGAACTTAAACCAGTTGAGCTATGTTCAAGTTAACATCCCCACTTAATTTATCCAAATTAGTTTTGAAAAGGCTTATAAATCTCCTTTTTTTTTTCTTTTCATTATCTGTAAAGTGTAAACTTGGTGACAGAAGAACAGAGAACATATGGCAGAGACTAAACCTGTAGCTCCTGATGGAGATGACCAGTTATTGGAATGGCCCAAGAAAGATAATCGCCGCTTTCTTCGTGCTGTCTATCGTGTCGGTGATCTCGATCGCACCATCAAGTATATCTTCTTCAATCTTACTCTAAATCTCTGTCTTCTCTCCTTCTTTATGGCAATGCATTTTCTATCTTCAGATTCTACACTAAATGCTTTGGAATGAAACTCCTGAGAAAAGTTGATATCCCAGAAAAGAAACTTACCAAAGTATTCATGGGGTTTGGCCCTGAGCAATCTTCATTCGTTCTCGAGTTGATTAACGGTATGTGGTTTTAATAAGAGTAATACTTAACTGCTAGCTAGACTGCAGTTATTTTCTACGCAGGGTGCCACGTGACAAACTTTTGAAAAACTAGTAGTCCAAGTATTGCTTTTTTAAGAATGTGTCTGAATCAAGTTATAATCTCTTATCTTGTTCCTTTTATCAATCCAAACTCATATTCAAAATATGCAGAACATGGGGTAACTTCATATGATATTGGCACTGGATTTGGACATTTTGCCATTGCTACTCAAGATGTGAGTGAAGCCAATTTCTTCATGGTTTTGGTTTTTTTTTCCCCTGCAAATTGAGATGAAGAATATTAACATCAAATTTTTGTTATTTTTCCAAGATCTACAAAATGGTTGAAAATGTTAAGGCCAAGGGTGGTGACAATATGATCATTAGAGAACCTTTTGAGGTGAAAGGAAGCTCCATTTTTGTGCTGGCCTATGTGAAAGATCCTGATGGTTATATCTTTGAGCTCATTCAAAGAGGCCCCACTCCTCAGCCACTCTGCCAAGTAATGCTTCGTGTTGGTGATTTACAACGATCTATCGACTTCTATCAGAGGGTAAAATCACAGATTGCAGTAATTTTCAGTTTAAGTAAACAAAAAAGTTACCATGTAAACACACATTCAATTTTCAGTTAAAAAGAATAAATAATACTTCTAGTCCTAACTTCTATGAAACTAATAATTTAATCCATGAACTTTAGTTTGAAACAATTTAGTTTTGTACTTTCATATTTTCAGCAATTTCCTTGAAGTCTAGCATATTTGTAACAATTTAATCTTTACTATGAAAGTTTTCATCAAAATGAAGGGTTATTTTTTATTATGTAATAATTTAATCTGTGTAGTTCAAGACTAAATTGTTATTTTCACGAAGTTTAGAGACCAGAAGTAGTTTTTAACTCCAAAAAATGGTAAAAAAGTCGACGTTATTAGAAGTTTGTTAAATTTCAATGATGATGGGAGTTTCTCATGAAAATGTTAACAAAACAAAACATAAAAAAGACCATCATTTACTGTCTTTCTTCTGTGCTACTAACATCTCATTTAATCACCATTTGGTTTTTGATTTTTGAGTTTTTTAAAACTAAACTTATAAACACTAATTTCACCTATTAATTTCTTTGTTTAAAAAACTAAAAGGGGTAGTTTTTAAAAACTTGTCTTTGTCATTGAAATTTGGCCAAGAAATCAAATGGTTTATTAAGAAAGATGAAAACTATTTTAAAGAAGTTGCAAGTGAACAAGCACAATTTTCAAAAAAACTAAATTGTTATCAAACGAGGCATAAGAAATTCTCGATCCTGAATCAGAATTTGTTTCCATACAGGCATTGGGAATGAGGGTACTTAACAAGGTTGAGAGTCTTGAACACAAGGTGAACTTTTACTTCCATCCATCTTAAGAACTGAAGGTTAATAAATGCTATAATTTTCAATGAACAAACAATTGAATTTGCAGTACGCCATAGCCATGATGGGGTATGCAGATGAGTTAGAGACAACTGCTCTGGAGTTGACTTACAATCATGGTGTGACAGAACATTCCAAGGGAAATGGATATTCCCAGGTCAACTTTTGTTAGGGGGTTCAATTTTCCTCTTAAGTTGGAGTTTGTCCTTGATTAAGAACCACTAATTTCCATACTAATGGTAAATTTTGGTTTTGATTTCAGGTTGCTATTGGCACTGATGATGTATACCAGAGTGCTGAGGTTGTTAATTTCATTACCAAAGAACTTGGAGGAAAGATAACCCAACAACCCAGTTTGGCTAGCCAAACCAACTCCAAGATTACCAGTTTTCTTGATCCTGATGGCTGGAAAATTGTGAGTTTTCCTTCCTCATATAAGATGATAAGATCAAATACTATTCTAAATCCGTCACACACTTCCAAGTCCTAATTTTTCGTTTGAAATTATAAAGGATTACTTTCAAGTAACTTGTGGGTTTTTGGTAAATTTGTTTACTTGTTTTGTCATGTTCAAGTAGTGCTTAAAACCAAGGTTTTCTCTTTTAGGTCCTAATTGACAATGAAGATTATCTGAAGGAACCGCAGTGAAGAAGGGTATTGACAGTGTAAACCTGATGTTGATTCTCTATTGAATGTTATACAATAAACAGCTTAAATATTTTCATCAATATCTATATGCAGCTGTCTGAGACTCTCTGAGTTATATAATTATAGTTCTTCTAGCTACTTTAAACCAAGAATGCAAGTGCAATATGCAAAAGTAAACAGCTTAAATGAAATATGCTTAAGGCAGACAATGGACTCATTGGCAACCAAAAACAGCTTTAAACTGCCTGGGATGTGAGATTTTGTTACTTTTTTTTTTTTCACTTCAAAAATTAACTCTATGTACATTTGTGACGAGAAGAATGTCTATAAGTGTTGTTACCTGAAGTGAAACTAGACTTTGAAGCCATCAGTATGAGAAGCAAAAGAAC

mRNA sequence

GTCCAACATTTACTAAACAAATTCATAGTTGTTCAATGCAATAATTAAACTGCACGTTATCAGATTCATCTCCACTGCACTTTTTGGTCTTTGGTCTGAACTTAAACCATGTAAACTTGGTGACAGAAGAACAGAGAACATATGGCAGAGACTAAACCTGTAGCTCCTGATGGAGATGACCAGTTATTGGAATGGCCCAAGAAAGATAATCGCCGCTTTCTTCGTGCTGTCTATCGTGTCGGTGATCTCGATCGCACCATCAAATTCTACACTAAATGCTTTGGAATGAAACTCCTGAGAAAAGTTGATATCCCAGAAAAGAAACTTACCAAAGTATTCATGGGGTTTGGCCCTGAGCAATCTTCATTCGTTCTCGAGTTGATTAACGAACATGGGGTAACTTCATATGATATTGGCACTGGATTTGGACATTTTGCCATTGCTACTCAAGATGCCAAGGGTGGTGACAATATGATCATTAGAGAACCTTTTGAGGTGAAAGGAAGCTCCATTTTTGTGCTGGCCTATGTGAAAGATCCTGATGGTTATATCTTTGAGCTCATTCAAAGAGGCCCCACTCCTCAGCCACTCTGCCAAGTAATGCTTCGTGTTGGTGATTTACAACGATCTATCGACTTCTATCAGAGGTACGCCATAGCCATGATGGGGTATGCAGATGAGTTAGAGACAACTGCTCTGGAGTTGACTTACAATCATGGTGTGACAGAACATTCCAAGGGAAATGGATATTCCCAGGTTGCTATTGGCACTGATGATGTATACCAGAGTGCTGAGGTCCTAATTGACAATGAAGATTATCTGAAGGAACCGCAGTGAAGAAGGGTATTGACAGTGTAAACCTGATGTTGATTCTCTATTGAATGTTATACAATAAACAGCTTAAATATTTTCATCAATATCTATATGCAGCTGTCTGAGACTCTCTGAGTTATATAATTATAGTTCTTCTAGCTACTTTAAACCAAGAATGCAAGTGCAATATGCAAAAGTAAACAGCTTAAATGAAATATGCTTAAGGCAGACAATGGACTCATTGGCAACCAAAAACAGCTTTAAACTGCCTGGGATGTGAGATTTTGTTACTTTTTTTTTTTTCACTTCAAAAATTAACTCTATGTACATTTGTGACGAGAAGAATGTCTATAAGTGTTGTTACCTGAAGTGAAACTAGACTTTGAAGCCATCAGTATGAGAAGCAAAAGAAC

Coding sequence (CDS)

ATGGCAGAGACTAAACCTGTAGCTCCTGATGGAGATGACCAGTTATTGGAATGGCCCAAGAAAGATAATCGCCGCTTTCTTCGTGCTGTCTATCGTGTCGGTGATCTCGATCGCACCATCAAATTCTACACTAAATGCTTTGGAATGAAACTCCTGAGAAAAGTTGATATCCCAGAAAAGAAACTTACCAAAGTATTCATGGGGTTTGGCCCTGAGCAATCTTCATTCGTTCTCGAGTTGATTAACGAACATGGGGTAACTTCATATGATATTGGCACTGGATTTGGACATTTTGCCATTGCTACTCAAGATGCCAAGGGTGGTGACAATATGATCATTAGAGAACCTTTTGAGGTGAAAGGAAGCTCCATTTTTGTGCTGGCCTATGTGAAAGATCCTGATGGTTATATCTTTGAGCTCATTCAAAGAGGCCCCACTCCTCAGCCACTCTGCCAAGTAATGCTTCGTGTTGGTGATTTACAACGATCTATCGACTTCTATCAGAGGTACGCCATAGCCATGATGGGGTATGCAGATGAGTTAGAGACAACTGCTCTGGAGTTGACTTACAATCATGGTGTGACAGAACATTCCAAGGGAAATGGATATTCCCAGGTTGCTATTGGCACTGATGATGTATACCAGAGTGCTGAGGTCCTAATTGACAATGAAGATTATCTGAAGGAACCGCAGTGA

Protein sequence

MAETKPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQDAKGGDNMIIREPFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQRYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVLIDNEDYLKEPQ

Homology

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BLAST of Lsi02G005970 vs. ExPASy Swiss-Prot
Match: O65398 (Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 4.6e-78
Identity = 147/231 (63.64%), Postives = 174/231 (75.32%), Query Frame = 0


Query: 15  LLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPEQS 74
           LLEWPKKDNRRFL  VYRVGDLDRTI+FYT+ FGMKLLRK DIPE+K +  F+GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 75  SFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIREPFEVKGSSIF 134
           +FV+EL   +GV+SYDIGTGFGHFAI+TQD         AKGG+  + REP  VKG    
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGN--VTREPGPVKGGG-S 126

Query: 135 VLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ----------------R 194
           V+A+VKDPDGY FELIQRGPTP+P CQVMLRVGDL R+I FY+                +
Sbjct: 127 VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYK 186

Query: 195 YAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVL 221
           Y I MMGYA+E E+  LELTYN+ VTE++KGN Y+Q+AIGTDDVY+S EV+
Sbjct: 187 YTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVI 234

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BLAST of Lsi02G005970 vs. ExPASy Swiss-Prot
Match: Q39366 (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.3e-77
Identity = 144/230 (62.61%), Postives = 175/230 (76.09%), Query Frame = 0


Query: 15  LLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPEQS 74
           L+EWPKKD RRFL  VYRVGDLDRTI+FYT+CFGMK+LRK D+PE+K +  F+GFGPE S
Sbjct: 7   LVEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETS 66

Query: 75  SFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIREPFEVKGSSIF 134
           +FV+EL   +GV+SYDIGTGFGHFAI+TQD         AKGG+  + REP  VKG    
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGN--VTREPGPVKGGG-S 126

Query: 135 VLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQR---------------Y 194
           V+A+VKDPDGY FELIQRGPTP+PLCQVMLRVGDL R++ F ++                
Sbjct: 127 VIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN 186

Query: 195 AIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVL 221
            I MMGYA+E E+  LELTYN+GVTE++KGN Y+Q+AIGTDDVY+SAEV+
Sbjct: 187 TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVV 233

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BLAST of Lsi02G005970 vs. ExPASy Swiss-Prot
Match: Q948T6 (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=1 SV=2)

HSP 1 Score: 286.2 bits (731), Expect = 3.3e-76
Identity = 144/237 (60.76%), Postives = 174/237 (73.42%), Query Frame = 0


Query: 8   APDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFM 67
           A    + +LEWPKKD +R L AVYRVGDLDRTIK YT+CFGMKLLRK D+PE+K T  F+
Sbjct: 7   AEKSPEVVLEWPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFL 66

Query: 68  GFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQDA-KGGDNM-------IIREPFEV 127
           GFGPE ++F LEL   +GV  YDIG GFGHFAIAT+D  K  + +       I REP  V
Sbjct: 67  GFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPV 126

Query: 128 KGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ----------- 187
           KG S  V+A+ +DPDGY+FELIQRGPTP+PLCQVMLRVGDL RSI FY+           
Sbjct: 127 KGGST-VIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKK 186

Query: 188 -----RYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVL 221
                +Y IAM+GYADE +TT +ELTYN+GVTE++KGN Y+QVAIGT+DVY+SAE +
Sbjct: 187 DVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAV 242

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BLAST of Lsi02G005970 vs. ExPASy Swiss-Prot
Match: Q8W593 (Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g67280 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 5.5e-71
Identity = 135/233 (57.94%), Postives = 161/233 (69.10%), Query Frame = 0


Query: 13  DQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPE 72
           D LL W K D RR L  VYRVGD+DRTIKFYT+C GMKLLRK DIPE+K T  F+G+GPE
Sbjct: 76  DDLLTWVKNDKRRMLHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPE 135

Query: 73  QSSFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIREPFEVKGSS 132
            S FV+EL   +GV  YDIG GFGHF IA  D         AKGG   + REP  VKG  
Sbjct: 136 DSHFVIELTYNYGVDKYDIGAGFGHFGIAVDDVAKTVELVKAKGG--KVSREPGPVKGGK 195

Query: 133 IFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ--------------- 192
             V+A+++DPDGY FEL++RGPTP+PLCQVMLRVGDL R+I FY+               
Sbjct: 196 T-VIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRAIKFYEKAFGMELLRTRDNPE 255

Query: 193 -RYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVL 221
            +Y IAMMGY  E +   LELTYN+GVTE+ KGN Y+Q+AIGTDDVY++AE +
Sbjct: 256 YKYTIAMMGYGPEDKFPVLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEAI 305

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BLAST of Lsi02G005970 vs. ExPASy Swiss-Prot
Match: Q9KT93 (Probable lactoylglutathione lyase OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=gloA PE=3 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 57/127 (44.88%), Postives = 74/127 (58.27%), Query Frame = 0


Query: 23  NRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPEQSSFVLELIN 82
           N R L  + RVGDLD++I+FYT+  GM LLRK +  E K T  F+G+G E    V+EL  
Sbjct: 3   NHRILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTY 62

Query: 83  EHGVTSYDIGTGFGHFAIATQD-------AKGGDNMIIREPFEVKGSSIFVLAYVKDPDG 142
             GV  Y+ G  +GH AI   D        K    ++ REP  VKG +  + A+VKDPDG
Sbjct: 63  NWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHI-AFVKDPDG 122

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BLAST of Lsi02G005970 vs. ExPASy TrEMBL
Match: A0A5D3BI05 (Glyoxalase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005690 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.9e-99
Identity = 182/245 (74.29%), Postives = 203/245 (82.86%), Query Frame = 0


Query: 1   MAETKPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEK 60
           M ETKPVAPDGD+QLLEWP KDNRRF+RAVYRVGDLDRTIKFYT+C GM+LLRK ++  +
Sbjct: 1   MTETKPVAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSNE 60

Query: 61  KLTKVFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNM 120
           K TK  MGFGPE+SSFVLEL  E GVTSYDIGTGFGHFAIATQD         AKGG+NM
Sbjct: 61  KHTKAIMGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQDVYKMVENVRAKGGENM 120

Query: 121 IIREPFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFY---- 180
           IIREPFE+KGS + +LAYVKDP+GYIFELIQRG TPQPLC +MLRV DLQRSIDFY    
Sbjct: 121 IIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQRSIDFYRKAL 180

Query: 181 ------------QRYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQ 221
                       Q+YAIA+MGYADELETTA+ELTYNHGVT+HSKGNGYSQVAIGTDDVY+
Sbjct: 181 GMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQVAIGTDDVYK 240

[Close]

BLAST of Lsi02G005970 vs. ExPASy TrEMBL
Match: E5GC58 (Glyoxalase I OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.9e-99
Identity = 182/245 (74.29%), Postives = 203/245 (82.86%), Query Frame = 0


Query: 1   MAETKPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEK 60
           M ETKPVAPDGD+QLLEWP KDNRRF+RAVYRVGDLDRTIKFYT+C GM+LLRK ++  +
Sbjct: 1   MTETKPVAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSNE 60

Query: 61  KLTKVFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNM 120
           K TK  MGFGPE+SSFVLEL  E GVTSYDIGTGFGHFAIATQD         AKGG+NM
Sbjct: 61  KHTKAIMGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQDVYKMVENVRAKGGENM 120

Query: 121 IIREPFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFY---- 180
           IIREPFE+KGS + +LAYVKDP+GYIFELIQRG TPQPLC +MLRV DLQRSIDFY    
Sbjct: 121 IIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQRSIDFYRKAL 180

Query: 181 ------------QRYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQ 221
                       Q+YAIA+MGYADELETTA+ELTYNHGVT+HSKGNGYSQVAIGTDDVY+
Sbjct: 181 GMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQVAIGTDDVYK 240

[Close]

BLAST of Lsi02G005970 vs. ExPASy TrEMBL
Match: A0A1S3B2D8 (Glyoxalase I OS=Cucumis melo OX=3656 GN=LOC103485234 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.9e-99
Identity = 182/245 (74.29%), Postives = 203/245 (82.86%), Query Frame = 0


Query: 1   MAETKPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEK 60
           M ETKPVAPDGD+QLLEWP KDNRRF+RAVYRVGDLDRTIKFYT+C GM+LLRK ++  +
Sbjct: 1   MTETKPVAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSNE 60

Query: 61  KLTKVFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNM 120
           K TK  MGFGPE+SSFVLEL  E GVTSYDIGTGFGHFAIATQD         AKGG+NM
Sbjct: 61  KHTKAIMGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQDVYKMVENVRAKGGENM 120

Query: 121 IIREPFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFY---- 180
           IIREPFE+KGS + +LAYVKDP+GYIFELIQRG TPQPLC +MLRV DLQRSIDFY    
Sbjct: 121 IIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQRSIDFYRKAL 180

Query: 181 ------------QRYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQ 221
                       Q+YAIA+MGYADELETTA+ELTYNHGVT+HSKGNGYSQVAIGTDDVY+
Sbjct: 181 GMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQVAIGTDDVYK 240

[Close]

BLAST of Lsi02G005970 vs. ExPASy TrEMBL
Match: A0A0A0LS05 (Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_1G062880 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 6.3e-94
Identity = 177/241 (73.44%), Postives = 197/241 (81.74%), Query Frame = 0


Query: 5   KPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTK 64
           K VAPDGD+QLLEWP KDNRRF+RAVYRVGDLDRTIKFYT+C  MKLLRK ++P +K TK
Sbjct: 6   KHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLEMKLLRKYEVPNEKHTK 65

Query: 65  VFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIRE 124
             MGFGPE+SSF+LEL +EHGVTSYDIGTGFGHFAIATQD         AKGG+NMIIRE
Sbjct: 66  ALMGFGPEESSFILELTHEHGVTSYDIGTGFGHFAIATQDVYKMVENVRAKGGENMIIRE 125

Query: 125 PFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFY-------- 184
           PFE+KGS  F+LAYVKDP+GYIFELIQR  T QPLC +MLRV DLQRSIDFY        
Sbjct: 126 PFELKGSPNFLLAYVKDPNGYIFELIQRVQT-QPLCHLMLRVADLQRSIDFYQKAMGMRV 185

Query: 185 --------QRYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEV 221
                   Q+YAIA+MGYADELETTALEL YNHGVT+HSKGNGYSQV IGTDDVY+SAE+
Sbjct: 186 LTQVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVVIGTDDVYKSAEI 245

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BLAST of Lsi02G005970 vs. ExPASy TrEMBL
Match: A0A6J1CEU9 (Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111010851 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 3.1e-93
Identity = 190/292 (65.07%), Postives = 205/292 (70.21%), Query Frame = 0


Query: 3   ETKPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKL 62
           +  P+APD D QLLEWPKKDNRR L AVY VGDLDRTIKFYT+CFGMKLLR  D+PE+K 
Sbjct: 4   QATPLAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKY 63

Query: 63  TKVFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMII 122
           +  FMGFGPEQSSFV+ELI  HGVTSYDIGTGFGHFAIATQD         AK  D+ II
Sbjct: 64  SNAFMGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDVYKMVENITAKSADS-II 123

Query: 123 REPFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ----- 182
           REPFE+KGSS FVLAYVKDPDGYIFELIQR PTPQPLC VMLRV DLQRSI+FY+     
Sbjct: 124 REPFELKGSS-FVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGM 183

Query: 183 -----------RYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSA 232
                      +YAIA+MGYADE ET ALELTYNHGVTEHSKGNGYSQVAIGTDDVY+SA
Sbjct: 184 RSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSA 243

[Close]

BLAST of Lsi02G005970 vs. NCBI nr
Match: XP_038895603.1 (LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Benincasa hispida])

HSP 1 Score: 385.2 bits (988), Expect = 4.0e-103
Identity = 202/294 (68.71%), Postives = 216/294 (73.47%), Query Frame = 0


Query: 1   MAETKPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEK 60
           MAET+PVAPDG DQ+LEWPKKDNRRFLR VYRVGDLDRTIKFYT+CFGM+LLRK ++ E+
Sbjct: 1   MAETRPVAPDGHDQMLEWPKKDNRRFLRTVYRVGDLDRTIKFYTECFGMELLRKNEVQEE 60

Query: 61  KLTKVFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQDA---------KGGDNM 120
           K TK FMG+GPEQSS VLELI EHGVTSY+IG GFGHFAIATQD          KGGDNM
Sbjct: 61  KHTKAFMGYGPEQSSIVLELIYEHGVTSYNIGNGFGHFAIATQDVYKMVENVKDKGGDNM 120

Query: 121 IIREPFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ--- 180
           IIREPFEVKGSSIFVLAYVKDPDGYIFELIQR PTPQPLC VMLRVGDLQRSIDFY+   
Sbjct: 121 IIREPFEVKGSSIFVLAYVKDPDGYIFELIQRDPTPQPLCHVMLRVGDLQRSIDFYKKAL 180

Query: 181 -------------RYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQ 232
                        +YAIAMMGYADELETTALELTYNHGVTEHSKG  YSQVAIGTDDVY+
Sbjct: 181 GMRVLNKVQSLEHKYAIAMMGYADELETTALELTYNHGVTEHSKGMXYSQVAIGTDDVYK 240

[Close]

BLAST of Lsi02G005970 vs. NCBI nr
Match: XP_008440988.1 (PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >ADN34057.1 lactoylglutathione lyase [Cucumis melo subsp. melo] >KAA0056849.1 putative lactoylglutathione lyase [Cucumis melo var. makuwa] >TYJ99352.1 putative lactoylglutathione lyase [Cucumis melo var. makuwa])

HSP 1 Score: 371.3 bits (952), Expect = 6.0e-99
Identity = 182/245 (74.29%), Postives = 203/245 (82.86%), Query Frame = 0


Query: 1   MAETKPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEK 60
           M ETKPVAPDGD+QLLEWP KDNRRF+RAVYRVGDLDRTIKFYT+C GM+LLRK ++  +
Sbjct: 1   MTETKPVAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSNE 60

Query: 61  KLTKVFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNM 120
           K TK  MGFGPE+SSFVLEL  E GVTSYDIGTGFGHFAIATQD         AKGG+NM
Sbjct: 61  KHTKAIMGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQDVYKMVENVRAKGGENM 120

Query: 121 IIREPFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFY---- 180
           IIREPFE+KGS + +LAYVKDP+GYIFELIQRG TPQPLC +MLRV DLQRSIDFY    
Sbjct: 121 IIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQRSIDFYRKAL 180

Query: 181 ------------QRYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQ 221
                       Q+YAIA+MGYADELETTA+ELTYNHGVT+HSKGNGYSQVAIGTDDVY+
Sbjct: 181 GMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQVAIGTDDVYK 240

[Close]

BLAST of Lsi02G005970 vs. NCBI nr
Match: XP_004145118.1 (lactoylglutathione lyase GLX1 [Cucumis sativus] >KGN64513.1 hypothetical protein Csa_013535 [Cucumis sativus])

HSP 1 Score: 353.6 bits (906), Expect = 1.3e-93
Identity = 177/241 (73.44%), Postives = 197/241 (81.74%), Query Frame = 0


Query: 5   KPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTK 64
           K VAPDGD+QLLEWP KDNRRF+RAVYRVGDLDRTIKFYT+C  MKLLRK ++P +K TK
Sbjct: 6   KHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLEMKLLRKYEVPNEKHTK 65

Query: 65  VFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIRE 124
             MGFGPE+SSF+LEL +EHGVTSYDIGTGFGHFAIATQD         AKGG+NMIIRE
Sbjct: 66  ALMGFGPEESSFILELTHEHGVTSYDIGTGFGHFAIATQDVYKMVENVRAKGGENMIIRE 125

Query: 125 PFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFY-------- 184
           PFE+KGS  F+LAYVKDP+GYIFELIQR  T QPLC +MLRV DLQRSIDFY        
Sbjct: 126 PFELKGSPNFLLAYVKDPNGYIFELIQRVQT-QPLCHLMLRVADLQRSIDFYQKAMGMRV 185

Query: 185 --------QRYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEV 221
                   Q+YAIA+MGYADELETTALEL YNHGVT+HSKGNGYSQV IGTDDVY+SAE+
Sbjct: 186 LTQVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVVIGTDDVYKSAEI 245

[Close]

BLAST of Lsi02G005970 vs. NCBI nr
Match: XP_022140116.1 (putative lactoylglutathione lyase [Momordica charantia])

HSP 1 Score: 351.3 bits (900), Expect = 6.4e-93
Identity = 190/292 (65.07%), Postives = 205/292 (70.21%), Query Frame = 0


Query: 3   ETKPVAPDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKL 62
           +  P+APD D QLLEWPKKDNRR L AVY VGDLDRTIKFYT+CFGMKLLR  D+PE+K 
Sbjct: 4   QATPLAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKY 63

Query: 63  TKVFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMII 122
           +  FMGFGPEQSSFV+ELI  HGVTSYDIGTGFGHFAIATQD         AK  D+ II
Sbjct: 64  SNAFMGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDVYKMVENITAKSADS-II 123

Query: 123 REPFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ----- 182
           REPFE+KGSS FVLAYVKDPDGYIFELIQR PTPQPLC VMLRV DLQRSI+FY+     
Sbjct: 124 REPFELKGSS-FVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGM 183

Query: 183 -----------RYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSA 232
                      +YAIA+MGYADE ET ALELTYNHGVTEHSKGNGYSQVAIGTDDVY+SA
Sbjct: 184 RSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSA 243

[Close]

BLAST of Lsi02G005970 vs. NCBI nr
Match: KAG7012363.1 (hypothetical protein SDJN02_25115, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 341.3 bits (874), Expect = 6.6e-90
Identity = 175/246 (71.14%), Postives = 195/246 (79.27%), Query Frame = 0


Query: 1   MAETKPV--APDGDDQLLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIP 60
           MAE KPV  APD D+QLLEWP KDNR FLRAV+RV DLDR I+F+T+CFGMKLLR  D+P
Sbjct: 1   MAEAKPVAMAPDADEQLLEWPMKDNRPFLRAVFRVSDLDRMIEFFTECFGMKLLRIRDVP 60

Query: 61  EKKLTKVFMGFGPEQSSFVLELINEHGVTSYDIGTGFGHFAIATQDA--------KGGDN 120
           E K T+ FMGFGPEQSSFVLEL   +GVTSYDIG GFGHFAIATQD           G  
Sbjct: 61  EAKCTRAFMGFGPEQSSFVLELNYSYGVTSYDIGGGFGHFAIATQDVYKMVENVRDKGAG 120

Query: 121 MIIREPFEVKGSSIFVLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ-- 180
            ++REPFEVKG SI VLA+VKDPDGY+FELIQRGPTPQPLCQVMLRVGDL+RSI+FY+  
Sbjct: 121 AVVREPFEVKGFSI-VLAFVKDPDGYMFELIQRGPTPQPLCQVMLRVGDLERSINFYEKA 180

Query: 181 --------------RYAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVY 221
                         +YAIAMMGYADELETT LELTYNHGVT+HSKGNGYSQVAIGTD+V 
Sbjct: 181 LGMRVLTRIESLEHKYAIAMMGYADELETTVLELTYNHGVTQHSKGNGYSQVAIGTDNVN 240

[Close]

BLAST of Lsi02G005970 vs. TAIR 10
Match: AT1G11840.2 (glyoxalase I homolog )

HSP 1 Score: 292.4 bits (747), Expect = 3.3e-79
Identity = 147/231 (63.64%), Postives = 174/231 (75.32%), Query Frame = 0


Query: 15  LLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPEQS 74
           LLEWPKKDNRRFL  VYRVGDLDRTI+FYT+ FGMKLLRK DIPE+K +  F+GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 75  SFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIREPFEVKGSSIF 134
           +FV+EL   +GV+SYDIGTGFGHFAI+TQD         AKGG+  + REP  VKG    
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGN--VTREPGPVKGGG-S 126

Query: 135 VLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ----------------R 194
           V+A+VKDPDGY FELIQRGPTP+P CQVMLRVGDL R+I FY+                +
Sbjct: 127 VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYK 186

Query: 195 YAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVL 221
           Y I MMGYA+E E+  LELTYN+ VTE++KGN Y+Q+AIGTDDVY+S EV+
Sbjct: 187 YTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVI 234

[Close]

BLAST of Lsi02G005970 vs. TAIR 10
Match: AT1G11840.3 (glyoxalase I homolog )

HSP 1 Score: 292.4 bits (747), Expect = 3.3e-79
Identity = 147/231 (63.64%), Postives = 174/231 (75.32%), Query Frame = 0


Query: 15  LLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPEQS 74
           LLEWPKKDNRRFL  VYRVGDLDRTI+FYT+ FGMKLLRK DIPE+K +  F+GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 75  SFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIREPFEVKGSSIF 134
           +FV+EL   +GV+SYDIGTGFGHFAI+TQD         AKGG+  + REP  VKG    
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGN--VTREPGPVKGGG-S 126

Query: 135 VLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ----------------R 194
           V+A+VKDPDGY FELIQRGPTP+P CQVMLRVGDL R+I FY+                +
Sbjct: 127 VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYK 186

Query: 195 YAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVL 221
           Y I MMGYA+E E+  LELTYN+ VTE++KGN Y+Q+AIGTDDVY+S EV+
Sbjct: 187 YTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVI 234

[Close]

BLAST of Lsi02G005970 vs. TAIR 10
Match: AT1G11840.1 (glyoxalase I homolog )

HSP 1 Score: 292.4 bits (747), Expect = 3.3e-79
Identity = 147/231 (63.64%), Postives = 174/231 (75.32%), Query Frame = 0


Query: 15  LLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPEQS 74
           LLEWPKKDNRRFL  VYRVGDLDRTI+FYT+ FGMKLLRK DIPE+K +  F+GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 75  SFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIREPFEVKGSSIF 134
           +FV+EL   +GV+SYDIGTGFGHFAI+TQD         AKGG+  + REP  VKG    
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGN--VTREPGPVKGGG-S 126

Query: 135 VLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ----------------R 194
           V+A+VKDPDGY FELIQRGPTP+P CQVMLRVGDL R+I FY+                +
Sbjct: 127 VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYK 186

Query: 195 YAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVL 221
           Y I MMGYA+E E+  LELTYN+ VTE++KGN Y+Q+AIGTDDVY+S EV+
Sbjct: 187 YTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVI 234

[Close]

BLAST of Lsi02G005970 vs. TAIR 10
Match: AT1G11840.4 (glyoxalase I homolog )

HSP 1 Score: 292.4 bits (747), Expect = 3.3e-79
Identity = 147/231 (63.64%), Postives = 174/231 (75.32%), Query Frame = 0


Query: 15  LLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPEQS 74
           LLEWPKKDNRRFL  VYRVGDLDRTI+FYT+ FGMKLLRK DIPE+K +  F+GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 75  SFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIREPFEVKGSSIF 134
           +FV+EL   +GV+SYDIGTGFGHFAI+TQD         AKGG+  + REP  VKG    
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGN--VTREPGPVKGGG-S 126

Query: 135 VLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ----------------R 194
           V+A+VKDPDGY FELIQRGPTP+P CQVMLRVGDL R+I FY+                +
Sbjct: 127 VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYK 186

Query: 195 YAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVL 221
           Y I MMGYA+E E+  LELTYN+ VTE++KGN Y+Q+AIGTDDVY+S EV+
Sbjct: 187 YTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVI 234

[Close]

BLAST of Lsi02G005970 vs. TAIR 10
Match: AT1G11840.6 (glyoxalase I homolog )

HSP 1 Score: 292.4 bits (747), Expect = 3.3e-79
Identity = 147/231 (63.64%), Postives = 174/231 (75.32%), Query Frame = 0


Query: 15  LLEWPKKDNRRFLRAVYRVGDLDRTIKFYTKCFGMKLLRKVDIPEKKLTKVFMGFGPEQS 74
           LLEWPKKDNRRFL  VYRVGDLDRTI+FYT+ FGMKLLRK DIPE+K +  F+GFGPE S
Sbjct: 46  LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 105

Query: 75  SFVLELINEHGVTSYDIGTGFGHFAIATQD---------AKGGDNMIIREPFEVKGSSIF 134
           +FV+EL   +GV+SYDIGTGFGHFAI+TQD         AKGG+  + REP  VKG    
Sbjct: 106 NFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGN--VTREPGPVKGGG-S 165

Query: 135 VLAYVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLQRSIDFYQ----------------R 194
           V+A+VKDPDGY FELIQRGPTP+P CQVMLRVGDL R+I FY+                +
Sbjct: 166 VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYK 225

Query: 195 YAIAMMGYADELETTALELTYNHGVTEHSKGNGYSQVAIGTDDVYQSAEVL 221
           Y I MMGYA+E E+  LELTYN+ VTE++KGN Y+Q+AIGTDDVY+S EV+
Sbjct: 226 YTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVI 273

The following BLAST results are available for this feature:

BLAST of Lsi02G005970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65398	4.6e-78	63.64	Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1	[more]
Q39366	2.3e-77	62.61	Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 ...	[more]
Q948T6	3.3e-76	60.76	Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=...	[more]
Q8W593	5.5e-71	57.94	Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q9KT93	1.1e-23	44.88	Probable lactoylglutathione lyase OS=Vibrio cholerae serotype O1 (strain ATCC 39...	[more]

BLAST of Lsi02G005970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BI05	2.9e-99	74.29	Glyoxalase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005690 ...	[more]
E5GC58	2.9e-99	74.29	Glyoxalase I OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A1S3B2D8	2.9e-99	74.29	Glyoxalase I OS=Cucumis melo OX=3656 GN=LOC103485234 PE=3 SV=1	[more]
A0A0A0LS05	6.3e-94	73.44	Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_1G062880 PE=3 SV=1	[more]
A0A6J1CEU9	3.1e-93	65.07	Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111010851 PE=3 SV=...	[more]

BLAST of Lsi02G005970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895603.1	4.0e-103	68.71	LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Benincasa hispida]	[more]
XP_008440988.1	6.0e-99	74.29	PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >ADN34057.1 lactoylg...	[more]
XP_004145118.1	1.3e-93	73.44	lactoylglutathione lyase GLX1 [Cucumis sativus] >KGN64513.1 hypothetical protein...	[more]
XP_022140116.1	6.4e-93	65.07	putative lactoylglutathione lyase [Momordica charantia]	[more]
KAG7012363.1	6.6e-90	71.14	hypothetical protein SDJN02_25115, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lsi02G005970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11840.2	3.3e-79	63.64	glyoxalase I homolog	[more]
AT1G11840.3	3.3e-79	63.64	glyoxalase I homolog	[more]
AT1G11840.1	3.3e-79	63.64	glyoxalase I homolog	[more]
AT1G11840.4	3.3e-79	63.64	glyoxalase I homolog	[more]
AT1G11840.6	3.3e-79	63.64	glyoxalase I homolog	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004360	Glyoxalase/fosfomycin resistance/dioxygenase domain	PFAM	PF00903	Glyoxalase	coord: 29..140 e-value: 1.5E-10 score: 41.3
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 146..231 e-value: 9.4E-15 score: 57.0
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 4..144 e-value: 1.4E-36 score: 127.8
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 25..223
IPR004361	Glyoxalase I	TIGRFAM	TIGR00068	TIGR00068	coord: 14..152 e-value: 3.1E-43 score: 144.8
None	No IPR available	PANTHER	PTHR46036	LACTOYLGLUTATHIONE LYASE	coord: 1..222
IPR018146	Glyoxalase I, conserved site	PROSITE	PS00935	GLYOXALASE_I_2	coord: 92..104
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 149..231 score: 12.140877
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 25..143 score: 19.026001

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G005970.1	Lsi02G005970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019243	methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004462	lactoylglutathione lyase activity
molecular_function	GO:0046872	metal ion binding