Lsi02G005930 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G005930
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr02: 5270963 .. 5272728 (-)
RNA-Seq Expression	Lsi02G005930
Synteny	Lsi02G005930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGTTTTAGTAAATTATCATGTTTCATTGTAATTTCTGTAATAAGCCCTCATTTAATCAAGATCAAGATCAAAATCAATCAATTCAATTGCATGGAGTCTTCTCTTTGGGTTTGCAATTTGTTGATTATTTTTGGTCAACTGCTCAGACCCAGAAGAGGAAACCGACATAATAAGTACAGGAGAAAGAATTGATAAAGATTAGATGATGCAAGGATTTGTAGCTGAAACAGAGGCCCAGAATGACGTAGTAGCTGAGTTCAAGCTTCATCTTCAACCCCATTTCAATGGCAGATTCTAAGCTTTCTCTGTTTTTTCTCTTCCTTTTTGTGTTTCTCACTGCTTCAGAAGGTACGGACAAGAAAATCTTCTAAAAAGTTGTAGAAGTTTCTCTTCTTCTTCTTCTTATTTTTCTCTCGTTGGGAGGGTTTTTGCAGGTAAATTTCCGCCATTGATTAAGGGGCAGAAGGTGGAGAAGGACGATAAGCAGCCATTTGTTGGGGTTAATATTGGGAGTGACGTTTCGAATCTATTGTCGCCTTCAGACTTGGTTTCTTTCTTGCAGTTTCAGAAAATCACTCACATTAGGCTTTACGATACGAACCCTGATATTCTCAAAGCCCTAAGCGGGTCAAAAATTCGAGTCATAATCAGTGTACCCAACAACCAACTTCTTGCAATTGGGTCTTCGAATGCCACCGCCGCCGCATGGATCGGTCGGAATGTGGTGGCGCACTACCCTCAGACACTAATTTCCGGCATTTCCGTCGGCGATGAGGTTTTAACCACTATCCCCTCTGCTTCCCCTTTGTTGCTTCCTGCTATTGAATCCCTTTACAATGCTCTCGTTGCTGCAAATCTTCAAACCCAAATCACCGTTTCGACTCCACACGCGGCTTCGATTATTCTCGACCCATTTCCGCCATCGCAGGCTTTCTTCAACCAGAGCTTGGTTCAGTTCGTACTTCCTCTGCTTCAGTTCCTTTCGAAAACTGGGTCGCCATTGATGATGAATTTTTACCCTTATTATGTTTTTATGCAAAACAAAGGGGTTGTTCCTCTTGATAATGCGCTCTTCAAACCCTTGACTCCGTCTAAAGAAATGGTCGATCCCAATACTCTGCTTCACTACACCAATGTCCTCGACGCCATGATTGACTCTGCTTATTTCTCGATGAAGAATCTCAATATCACAGACGTTGTGGTTCTCGTTACTGAAAGTGGGTGGCCTTCGAAGGGCGATTCGAAAGAGCCGTACGCATCAATCGACAACGCCGACACTTTCAATTCGAATCTAATCAAACATATTCTTGACCGGAGTGGAACCCCATTTCACCCTGAAGTTACTTCGAGTGTGTATATCTACGAGTTGTTCAATGAAGATTTACGGTCACCGCCGGTGTCGGAGGCGAATTGGGGGCTGTTTTACGGCAACTCAACGCCGGTTTATTTGCTTCATGTATCGGGAAGTGGGACGTTTTTGGCTAATGATACAACGAATCAGACTTATTGTATTGCCATGGATGAGTTTGATGCAAAGACATTGCAAACGGCTTTAGATTGGGCTTGTGGACCTGGAAAAGCTAATTGTACAGAGATTCAGCCTGGTGAGGCTTGTTATCAGCCTAACAATGTGAAGAACCATGCTTCTTATGCATTTGATAGCTATTACCAGAAAGAAGGCAAAACTGCTGGATCTTGTGATTTCAAGGGTGTGGCTATGATCACCACCACTGACCCAAGTGAGTTTTCATCTCTTTTGTCTTAA

mRNA sequence

GTGTTTTAGTAAATTATCATGTTTCATTGTAATTTCTGTAATAAGCCCTCATTTAATCAAGATCAAGATCAAAATCAATCAATTCAATTGCATGGAGTCTTCTCTTTGGGTTTGCAATTTGTTGATTATTTTTGGTCAACTGCTCAGACCCAGAAGAGGAAACCGACATAATAAGTACAGGAGAAAGAATTGATAAAGATTAGATGATGCAAGGATTTGTAGCTGAAACAGAGGCCCAGAATGACGTAGTAGCTGAGTTCAAGCTTCATCTTCAACCCCATTTCAATGGCAGATTCTAAGCTTTCTCTGTTTTTTCTCTTCCTTTTTGTGTTTCTCACTGCTTCAGAAGGTAAATTTCCGCCATTGATTAAGGGGCAGAAGGTGGAGAAGGACGATAAGCAGCCATTTGTTGGGGTTAATATTGGGAGTGACGTTTCGAATCTATTGTCGCCTTCAGACTTGGTTTCTTTCTTGCAGTTTCAGAAAATCACTCACATTAGGCTTTACGATACGAACCCTGATATTCTCAAAGCCCTAAGCGGGTCAAAAATTCGAGTCATAATCAGTGTACCCAACAACCAACTTCTTGCAATTGGGTCTTCGAATGCCACCGCCGCCGCATGGATCGGTCGGAATGTGGTGGCGCACTACCCTCAGACACTAATTTCCGGCATTTCCGTCGGCGATGAGGTTTTAACCACTATCCCCTCTGCTTCCCCTTTGTTGCTTCCTGCTATTGAATCCCTTTACAATGCTCTCGTTGCTGCAAATCTTCAAACCCAAATCACCGTTTCGACTCCACACGCGGCTTCGATTATTCTCGACCCATTTCCGCCATCGCAGGCTTTCTTCAACCAGAGCTTGGTTCAGTTCGTACTTCCTCTGCTTCAGTTCCTTTCGAAAACTGGGTCGCCATTGATGATGAATTTTTACCCTTATTATGTTTTTATGCAAAACAAAGGGGTTGTTCCTCTTGATAATGCGCTCTTCAAACCCTTGACTCCGTCTAAAGAAATGGTCGATCCCAATACTCTGCTTCACTACACCAATGTCCTCGACGCCATGATTGACTCTGCTTATTTCTCGATGAAGAATCTCAATATCACAGACGTTGTGGTTCTCGTTACTGAAAGTGGGTGGCCTTCGAAGGGCGATTCGAAAGAGCCGTACGCATCAATCGACAACGCCGACACTTTCAATTCGAATCTAATCAAACATATTCTTGACCGGAGTGGAACCCCATTTCACCCTGAAGTTACTTCGAGTGTGTATATCTACGAGTTGTTCAATGAAGATTTACGGTCACCGCCGGTGTCGGAGGCGAATTGGGGGCTGTTTTACGGCAACTCAACGCCGGTTTATTTGCTTCATGTATCGGGAAGTGGGACGTTTTTGGCTAATGATACAACGAATCAGACTTATTGTATTGCCATGGATGAGTTTGATGCAAAGACATTGCAAACGGCTTTAGATTGGGCTTGTGGACCTGGAAAAGCTAATTGTACAGAGATTCAGCCTGGTGAGGCTTGTTATCAGCCTAACAATGTGAAGAACCATGCTTCTTATGCATTTGATAGCTATTACCAGAAAGAAGGCAAAACTGCTGGATCTTGTGATTTCAAGGGTGTGGCTATGATCACCACCACTGACCCAAGTGAGTTTTCATCTCTTTTGTCTTAA

Coding sequence (CDS)

ATGGCAGATTCTAAGCTTTCTCTGTTTTTTCTCTTCCTTTTTGTGTTTCTCACTGCTTCAGAAGGTAAATTTCCGCCATTGATTAAGGGGCAGAAGGTGGAGAAGGACGATAAGCAGCCATTTGTTGGGGTTAATATTGGGAGTGACGTTTCGAATCTATTGTCGCCTTCAGACTTGGTTTCTTTCTTGCAGTTTCAGAAAATCACTCACATTAGGCTTTACGATACGAACCCTGATATTCTCAAAGCCCTAAGCGGGTCAAAAATTCGAGTCATAATCAGTGTACCCAACAACCAACTTCTTGCAATTGGGTCTTCGAATGCCACCGCCGCCGCATGGATCGGTCGGAATGTGGTGGCGCACTACCCTCAGACACTAATTTCCGGCATTTCCGTCGGCGATGAGGTTTTAACCACTATCCCCTCTGCTTCCCCTTTGTTGCTTCCTGCTATTGAATCCCTTTACAATGCTCTCGTTGCTGCAAATCTTCAAACCCAAATCACCGTTTCGACTCCACACGCGGCTTCGATTATTCTCGACCCATTTCCGCCATCGCAGGCTTTCTTCAACCAGAGCTTGGTTCAGTTCGTACTTCCTCTGCTTCAGTTCCTTTCGAAAACTGGGTCGCCATTGATGATGAATTTTTACCCTTATTATGTTTTTATGCAAAACAAAGGGGTTGTTCCTCTTGATAATGCGCTCTTCAAACCCTTGACTCCGTCTAAAGAAATGGTCGATCCCAATACTCTGCTTCACTACACCAATGTCCTCGACGCCATGATTGACTCTGCTTATTTCTCGATGAAGAATCTCAATATCACAGACGTTGTGGTTCTCGTTACTGAAAGTGGGTGGCCTTCGAAGGGCGATTCGAAAGAGCCGTACGCATCAATCGACAACGCCGACACTTTCAATTCGAATCTAATCAAACATATTCTTGACCGGAGTGGAACCCCATTTCACCCTGAAGTTACTTCGAGTGTGTATATCTACGAGTTGTTCAATGAAGATTTACGGTCACCGCCGGTGTCGGAGGCGAATTGGGGGCTGTTTTACGGCAACTCAACGCCGGTTTATTTGCTTCATGTATCGGGAAGTGGGACGTTTTTGGCTAATGATACAACGAATCAGACTTATTGTATTGCCATGGATGAGTTTGATGCAAAGACATTGCAAACGGCTTTAGATTGGGCTTGTGGACCTGGAAAAGCTAATTGTACAGAGATTCAGCCTGGTGAGGCTTGTTATCAGCCTAACAATGTGAAGAACCATGCTTCTTATGCATTTGATAGCTATTACCAGAAAGAAGGCAAAACTGCTGGATCTTGTGATTTCAAGGGTGTGGCTATGATCACCACCACTGACCCAAGTGAGTTTTCATCTCTTTTGTCTTAA

Protein sequence

MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLVSFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVAHYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSLLS

Homology

[Close]

BLAST of Lsi02G005930 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 741.5 bits (1913), Expect = 5.8e-213
Identity = 358/455 (78.68%), Postives = 411/455 (90.33%), Query Frame = 0


Query: 8   LFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLVSFLQFQK 67
           LFF F  + ++A+      L    KV++ DK PFVG NIG+DVSNLLSP++LV FLQ QK
Sbjct: 13  LFFFFTLLLISANSSS---LSHNIKVQEQDKDPFVGFNIGTDVSNLLSPTELVKFLQAQK 72

Query: 68  ITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVAHYPQTLI 127
           + H+RLYD +P++LKAL+ +K+RVIISVPNNQLLAIGSSN+TAA+WIGRNVVA+YP+TLI
Sbjct: 73  VNHVRLYDADPELLKALAKTKVRVIISVPNNQLLAIGSSNSTAASWIGRNVVAYYPETLI 132

Query: 128 SGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQA 187
           + ISVGDEVLTT+PS++PLLLPAIESLYNALVA+NL TQI VSTPHAASI+LD FPPSQA
Sbjct: 133 TAISVGDEVLTTVPSSAPLLLPAIESLYNALVASNLHTQIKVSTPHAASIMLDTFPPSQA 192

Query: 188 FFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEMVDP 247
           +FNQ+    ++PLLQFLSKTGSPLMMN YPYYV+MQNKGVVPLDN LF+PLTPSKEMVDP
Sbjct: 193 YFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYVYMQNKGVVPLDNCLFEPLTPSKEMVDP 252

Query: 248 NTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSN 307
           NTLLHYTNVLDAM+D+AY SMKNLN++DV VLVTESGWPSKGDSKEPYA+IDNADT+NSN
Sbjct: 253 NTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLVTESGWPSKGDSKEPYATIDNADTYNSN 312

Query: 308 LIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYLLHVSGSG 367
           LIKH+ DR+GTP HPE+TSSVYIYELFNEDLR+PPVSEA+WGLFYGNSTPVYLLHVSGSG
Sbjct: 313 LIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRAPPVSEASWGLFYGNSTPVYLLHVSGSG 372

Query: 368 TFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASY 427
           TFLANDTTNQTYCIAMD  DAKTLQ ALDWACGPG++NC+EIQPGE+CYQPNNVK HAS+
Sbjct: 373 TFLANDTTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASF 432

Query: 428 AFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           AF+SYYQKEG+ +GSCDFKGVAMITTTDPS  S +
Sbjct: 433 AFNSYYQKEGRASGSCDFKGVAMITTTDPSHGSCI 464

[Close]

BLAST of Lsi02G005930 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 514.2 bits (1323), Expect = 1.5e-144
Identity = 247/447 (55.26%), Postives = 336/447 (75.17%), Query Frame = 0


Query: 11  LFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLVSFLQFQKITH 70
           L LF+FL AS                 +   +GVNIG++V+N+ SP+ +V+ L+ Q I  
Sbjct: 5   LLLFLFLFASSAL-------------SQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINR 64

Query: 71  IRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVAHYPQTLISGI 130
           +RLYD +  +L A + + ++VIISVPN+QLL I  SNATAA W+ RNV A+YP T I+ I
Sbjct: 65  VRLYDADRSMLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTI 124

Query: 131 SVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQAFFN 190
           +VG EVLT++ +A+ +L+ A++ +  ALV ANL  QI VSTPH+++IILD FPPSQAFFN
Sbjct: 125 AVGSEVLTSLTNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN 184

Query: 191 QSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEMVDPNTL 250
           ++    ++PLL+FL  TGSPL++N YPY+ ++Q+ GV+PLD ALF+PL  +KE VD NTL
Sbjct: 185 KTWDPVIVPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTL 244

Query: 251 LHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSNLIK 310
           LHYTNV DA++D+AYF+M  LN T++ ++VTESGWPSKG   E  A+++NA+T+NSNLI+
Sbjct: 245 LHYTNVFDAIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQ 304

Query: 311 HILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYLLHVSGSGTFL 370
           H+++++GTP HP    + YIYEL+NED R  PVSE NWGLFY N TPVY L ++G+G  L
Sbjct: 305 HVINKTGTPKHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAIL 364

Query: 371 ANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFD 430
           ANDTTNQT+CIA ++ D K LQ ALDWACGPGK +C+ +  GE+CY+P++V  H++YAF+
Sbjct: 365 ANDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFN 424

Query: 431 SYYQKEGKTAGSCDFKGVAMITTTDPS 458
           +YYQK GK +GSCDFKGVA +TTTDPS
Sbjct: 425 AYYQKMGKASGSCDFKGVATVTTTDPS 438

[Close]

BLAST of Lsi02G005930 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 511.1 bits (1315), Expect = 1.3e-143
Identity = 250/454 (55.07%), Postives = 339/454 (74.67%), Query Frame = 0


Query: 4   SKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLVSFL 63
           S L L  L L + + AS    PP          D+  ++GVNIG+D+S++  P+ +V+ L
Sbjct: 3   SLLHLLLLSLSLLVLASASPSPPA---------DEGSYIGVNIGTDLSDMPHPTQVVALL 62

Query: 64  QFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVAHYP 123
           + Q+I HIRLY+ +P +L AL+ + I+VIIS+PN+QLL IG SN+TAA W+ RNV+AHYP
Sbjct: 63  KAQEIRHIRLYNADPGLLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYP 122

Query: 124 QTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFP 183
            T+I+ +SVG EVLT++ +A+P+L+ AI++++ AL++ANL   I VSTP + S+ILDPFP
Sbjct: 123 ATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFP 182

Query: 184 PSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKE 243
           PSQAFFN+SL   ++PLL FL  T S LM+N YPY  +MQ+ GV+PLD ALFKP+ P+KE
Sbjct: 183 PSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKE 242

Query: 244 MVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDNADT 303
            VD NTL+ Y+N  DAM+D+ YF+M  LN T++ VLVTESGWPSKG++ EP A++DNA+T
Sbjct: 243 AVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANT 302

Query: 304 FNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYLLHV 363
           +NSNLI+H+L+++GTP  P +  S YIYEL+NED ++  +SE NWGLF  N  PVY+L +
Sbjct: 303 YNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDTKA-GLSEKNWGLFNANGEPVYVLRL 362

Query: 364 SGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKN 423
           + SG+ LANDTTNQTYC A +  D K LQ ALDWACGPGK +C+ I+ GE CY+P+NV  
Sbjct: 363 TNSGSVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVA 422

Query: 424 HASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPS 458
           HA+YAFD+YY + G    +C+F GVA ITTTDPS
Sbjct: 423 HANYAFDTYYHQTGNNPDACNFNGVASITTTDPS 446

[Close]

BLAST of Lsi02G005930 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 4.0e-121
Identity = 211/417 (50.60%), Postives = 293/417 (70.26%), Query Frame = 0


Query: 41  FVGVNIGSDVSNLLSPSDLVSFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQL 100
           F+GVNIG+D++N+  PSD+V+ L+ Q+ITH+RLYD N  +LKA + + I V++ V N ++
Sbjct: 25  FIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFANTSIEVMVGVTNEEI 84

Query: 101 LAIGSSNATAAAWIGRNVVAHYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVA 160
           L IG   + AAAW+ +NV A+ P T I+ I+VG EVLTTIP  +P+L  A+ +++ ALVA
Sbjct: 85  LKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPILASALNNIHKALVA 144

Query: 161 ANLQTQITVSTPHAASIILDPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYV 220
           +NL  ++ VS+P +  I+  PFPPS + F+ S    V  LLQFL  TGS  M+N YPYY 
Sbjct: 145 SNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLKNTGSFFMLNAYPYYG 204

Query: 221 FMQNKGVVPLDNALFKPLTPSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLV 280
           +    G+ PLD ALFK L+P K++VDPNTLLHY ++ DAM+D+AY+SM+ LN + + V+V
Sbjct: 205 YTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYSMEALNFSKIPVVV 264

Query: 281 TESGWPSKGDSKEPYASIDNADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRS 340
           TE+GWPS G S E  A++ NA+TFN+NLIK +L+ SG P  P++  + YIYEL+NED RS
Sbjct: 265 TETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIPINTYIYELYNEDKRS 324

Query: 341 PPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACG 400
            PVSE NWG+ + N T VY L +SG  +  A + ++  +C+A  + D   L   L+WACG
Sbjct: 325 GPVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSM-FCVAKADADDDKLVDGLNWACG 384

Query: 401 PGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPS 458
            G+ANC  IQPG+ CY PN+VK+HAS+AF+ YYQK     G+CDF G A+ TT DPS
Sbjct: 385 QGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of Lsi02G005930 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 4.0e-89
Identity = 170/446 (38.12%), Postives = 258/446 (57.85%), Query Frame = 0


Query: 42  VGVNIGSDVSNLLSPSDLVSFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLL 101
           +G+  G +  NL SP+ +   +Q   I  +R+YD N D+LKA + + I ++I VPN  LL
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADLL 86

Query: 102 AIGSSNATAAAWIGRNVVAHYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAA 161
           A     +    W+  N++ +YP T I+ ISVG EV     +A+ L+LPA+ +++ AL  +
Sbjct: 87  AFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTALKKS 146

Query: 162 NLQTQITVSTPHAASIILDPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVF 221
            L  +I +S+ H+ +I+   FPPS A F++    F+ P+L+FL +  SP M++ YPYY +
Sbjct: 147 GLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLYPYYAY 206

Query: 222 MQNKGVVPLDNALFKPLTPSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVT 281
             +   VPL+ ALF+    S ++VDP T L Y+N+ DA +D+ YF++  ++   V V+VT
Sbjct: 207 RDSTEKVPLEYALFE---SSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVT 266

Query: 282 ESGWPSKGDSKEPYASIDNADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSP 341
           ESGWPSKG  KE  A+ +NA  +N+NLI+H++   GTP  P     VY++ LFNE+ +  
Sbjct: 267 ESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPG 326

Query: 342 PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQT----------------------- 401
             SE NWG+FY N T VY L  +G  T   + T + T                       
Sbjct: 327 IESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGG 386

Query: 402 -------YCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDS 458
                  +CIA  +     LQTALDWACGPG  +C+ +QP + C++P+ V +HASYAF++
Sbjct: 387 GGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNT 446

[Close]

BLAST of Lsi02G005930 vs. ExPASy TrEMBL
Match: A0A0A0LX25 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G062370 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 4.7e-250
Identity = 432/462 (93.51%), Postives = 446/462 (96.54%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60
           MADSK SLFFLFLFVFLT SEGKFPPLIKG KV+KDDKQPFVGVNIGSDVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFVFLTVSEGKFPPLIKGVKVDKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVA 120
           SFL FQKITHIRLYDTNPDILKAL GSKIRV+ISVPNNQLLAIG SN TAAAWI RNVVA
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWISRNVVA 120

Query: 121 HYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
           +YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360

Query: 361 LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNN 420
           LHVSGSGTFLANDTTNQTYCIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNN
Sbjct: 361 LHVSGSGTFLANDTTNQTYCIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNN 420

Query: 421 VKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           VKNHASYAFDSYYQKEGKT+GSCDFKG+AMITTTDPS  S +
Sbjct: 421 VKNHASYAFDSYYQKEGKTSGSCDFKGLAMITTTDPSHGSCI 462

[Close]

BLAST of Lsi02G005930 vs. ExPASy TrEMBL
Match: A0A6J1CDL3 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010711 PE=3 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.4e-249
Identity = 433/463 (93.52%), Postives = 452/463 (97.62%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKF-PPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDL 60
           MA+SK  LFFLF FVFLTASEGKF  PLIKGQK+EK+D+QPFVGVNIGSDVSNLLSPSDL
Sbjct: 1   MAESKFHLFFLFFFVFLTASEGKFQKPLIKGQKLEKEDRQPFVGVNIGSDVSNLLSPSDL 60

Query: 61  VSFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVV 120
           VSFL+FQKITHIRLYD NP+ILKALSG+KIRVIISVPNNQL+AIGSSNATAAAWIGRNVV
Sbjct: 61  VSFLRFQKITHIRLYDANPEILKALSGTKIRVIISVPNNQLIAIGSSNATAAAWIGRNVV 120

Query: 121 AHYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIIL 180
           A+YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALV+ANLQTQI +STPHAASIIL
Sbjct: 121 AYYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVSANLQTQIPISTPHAASIIL 180

Query: 181 DPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLT 240
           DPFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDN+LFKPLT
Sbjct: 181 DPFPPSQAFFNQSLVQFMLPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNSLFKPLT 240

Query: 241 PSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASID 300
           PSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYA+ID
Sbjct: 241 PSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYATID 300

Query: 301 NADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVY 360
           NADTFNSNLIKHILDRSGTPFHPE+TSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVY
Sbjct: 301 NADTFNSNLIKHILDRSGTPFHPEITSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVY 360

Query: 361 LLHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPN 420
           LLHV+GSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGE CYQPN
Sbjct: 361 LLHVTGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEPCYQPN 420

Query: 421 NVKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           NVKNHASYAFDSYYQKEGKT+GSCDFKGVAMITTTDPS  S +
Sbjct: 421 NVKNHASYAFDSYYQKEGKTSGSCDFKGVAMITTTDPSHGSCI 463

[Close]

BLAST of Lsi02G005930 vs. ExPASy TrEMBL
Match: A0A1S3B1W0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103485208 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 7.5e-248
Identity = 428/462 (92.64%), Postives = 443/462 (95.89%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60
           MADSK SLFFLFLF FLT SEGKF PLI G KVEKDDKQPFVGVNIGSDVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFAFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVA 120
           SFL FQKITHIRLYDTNPDILKAL GSKIRV+ISVPNNQLLAIG SN TAAAWI RNVVA
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 HYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
           +YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360

Query: 361 LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNN 420
           LHVSGSGTFLANDTTNQT+CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNN
Sbjct: 361 LHVSGSGTFLANDTTNQTFCIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNN 420

Query: 421 VKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           VKNHASYAFDSYYQKEGKT+GSCDFKG+AMITTTDPS  S +
Sbjct: 421 VKNHASYAFDSYYQKEGKTSGSCDFKGLAMITTTDPSHGSCI 462

[Close]

BLAST of Lsi02G005930 vs. ExPASy TrEMBL
Match: A0A5A7UQD8 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G00150 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 7.5e-248
Identity = 428/462 (92.64%), Postives = 443/462 (95.89%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60
           MADSK SLFFLFLF FLT SEGKF PLI G KVEKDDKQPFVGVNIGSDVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFAFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVA 120
           SFL FQKITHIRLYDTNPDILKAL GSKIRV+ISVPNNQLLAIG SN TAAAWI RNVVA
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 HYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
           +YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360

Query: 361 LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNN 420
           LHVSGSGTFLANDTTNQT+CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNN
Sbjct: 361 LHVSGSGTFLANDTTNQTFCIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNN 420

Query: 421 VKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           VKNHASYAFDSYYQKEGKT+GSCDFKG+AMITTTDPS  S +
Sbjct: 421 VKNHASYAFDSYYQKEGKTSGSCDFKGLAMITTTDPSHGSCI 462

[Close]

BLAST of Lsi02G005930 vs. ExPASy TrEMBL
Match: E5GC60 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 7.5e-248
Identity = 428/462 (92.64%), Postives = 443/462 (95.89%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60
           MADSK SLFFLFLF FLT SEGKF PLI G KVEKDDKQPFVGVNIGSDVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFAFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVA 120
           SFL FQKITHIRLYDTNPDILKAL GSKIRV+ISVPNNQLLAIG SN TAAAWI RNVVA
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 HYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
           +YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360

Query: 361 LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNN 420
           LHVSGSGTFLANDTTNQT+CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNN
Sbjct: 361 LHVSGSGTFLANDTTNQTFCIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNN 420

Query: 421 VKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           VKNHASYAFDSYYQKEGKT+GSCDFKG+AMITTTDPS  S +
Sbjct: 421 VKNHASYAFDSYYQKEGKTSGSCDFKGLAMITTTDPSHGSCI 462

[Close]

BLAST of Lsi02G005930 vs. NCBI nr
Match: XP_038895143.1 (glucan endo-1,3-beta-glucosidase 1 [Benincasa hispida])

HSP 1 Score: 877.1 bits (2265), Expect = 6.7e-251
Identity = 435/462 (94.16%), Postives = 448/462 (96.97%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60
           MADSK SLFFLFLFVFLT SEGKFPPLIKG+KV+KDDKQPF+GVNIGSDVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFVFLTVSEGKFPPLIKGEKVQKDDKQPFIGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVA 120
           SFL FQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIG SN TAAAWI RNVVA
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGFSNTTAAAWIDRNVVA 120

Query: 121 HYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
           +YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANL TQITVSTPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITVSTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDV+VLVTESGWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVMVLVTESGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360
           AD FNSNLIKHILDRSGTPFHPE+TSSVYIYELFNEDLRSPP+SEANWGLFYGNSTPVYL
Sbjct: 301 ADNFNSNLIKHILDRSGTPFHPEITSSVYIYELFNEDLRSPPLSEANWGLFYGNSTPVYL 360

Query: 361 LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNN 420
           LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEI PGE CYQPNN
Sbjct: 361 LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEILPGEVCYQPNN 420

Query: 421 VKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           VKNHASYAFDSYYQKEGKTAGSCDFKG+AMITTTDPS  S +
Sbjct: 421 VKNHASYAFDSYYQKEGKTAGSCDFKGLAMITTTDPSHGSCI 462

[Close]

BLAST of Lsi02G005930 vs. NCBI nr
Match: XP_004145115.1 (glucan endo-1,3-beta-glucosidase 1 [Cucumis sativus] >KGN64511.1 hypothetical protein Csa_013695 [Cucumis sativus])

HSP 1 Score: 873.2 bits (2255), Expect = 9.7e-250
Identity = 432/462 (93.51%), Postives = 446/462 (96.54%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60
           MADSK SLFFLFLFVFLT SEGKFPPLIKG KV+KDDKQPFVGVNIGSDVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFVFLTVSEGKFPPLIKGVKVDKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVA 120
           SFL FQKITHIRLYDTNPDILKAL GSKIRV+ISVPNNQLLAIG SN TAAAWI RNVVA
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWISRNVVA 120

Query: 121 HYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
           +YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360

Query: 361 LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNN 420
           LHVSGSGTFLANDTTNQTYCIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNN
Sbjct: 361 LHVSGSGTFLANDTTNQTYCIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNN 420

Query: 421 VKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           VKNHASYAFDSYYQKEGKT+GSCDFKG+AMITTTDPS  S +
Sbjct: 421 VKNHASYAFDSYYQKEGKTSGSCDFKGLAMITTTDPSHGSCI 462

[Close]

BLAST of Lsi02G005930 vs. NCBI nr
Match: XP_022139909.1 (glucan endo-1,3-beta-glucosidase 1 [Momordica charantia] >XP_022139910.1 glucan endo-1,3-beta-glucosidase 1 [Momordica charantia])

HSP 1 Score: 871.7 bits (2251), Expect = 2.8e-249
Identity = 433/463 (93.52%), Postives = 452/463 (97.62%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKF-PPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDL 60
           MA+SK  LFFLF FVFLTASEGKF  PLIKGQK+EK+D+QPFVGVNIGSDVSNLLSPSDL
Sbjct: 1   MAESKFHLFFLFFFVFLTASEGKFQKPLIKGQKLEKEDRQPFVGVNIGSDVSNLLSPSDL 60

Query: 61  VSFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVV 120
           VSFL+FQKITHIRLYD NP+ILKALSG+KIRVIISVPNNQL+AIGSSNATAAAWIGRNVV
Sbjct: 61  VSFLRFQKITHIRLYDANPEILKALSGTKIRVIISVPNNQLIAIGSSNATAAAWIGRNVV 120

Query: 121 AHYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIIL 180
           A+YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALV+ANLQTQI +STPHAASIIL
Sbjct: 121 AYYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVSANLQTQIPISTPHAASIIL 180

Query: 181 DPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLT 240
           DPFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDN+LFKPLT
Sbjct: 181 DPFPPSQAFFNQSLVQFMLPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNSLFKPLT 240

Query: 241 PSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASID 300
           PSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYA+ID
Sbjct: 241 PSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYATID 300

Query: 301 NADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVY 360
           NADTFNSNLIKHILDRSGTPFHPE+TSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVY
Sbjct: 301 NADTFNSNLIKHILDRSGTPFHPEITSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVY 360

Query: 361 LLHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPN 420
           LLHV+GSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGE CYQPN
Sbjct: 361 LLHVTGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEPCYQPN 420

Query: 421 NVKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           NVKNHASYAFDSYYQKEGKT+GSCDFKGVAMITTTDPS  S +
Sbjct: 421 NVKNHASYAFDSYYQKEGKTSGSCDFKGVAMITTTDPSHGSCI 463

[Close]

BLAST of Lsi02G005930 vs. NCBI nr
Match: XP_008440956.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 1 [Cucumis melo] >XP_008440965.1 PREDICTED: glucan endo-1,3-beta-glucosidase 1 [Cucumis melo] >ADN34059.1 glucan endo-13-beta-glucosidase [Cucumis melo subsp. melo] >KAA0056847.1 glucan endo-1,3-beta-glucosidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 865.9 bits (2236), Expect = 1.6e-247
Identity = 428/462 (92.64%), Postives = 443/462 (95.89%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60
           MADSK SLFFLFLF FLT SEGKF PLI G KVEKDDKQPFVGVNIGSDVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFAFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVA 120
           SFL FQKITHIRLYDTNPDILKAL GSKIRV+ISVPNNQLLAIG SN TAAAWI RNVVA
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 HYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
           +YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360

Query: 361 LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNN 420
           LHVSGSGTFLANDTTNQT+CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNN
Sbjct: 361 LHVSGSGTFLANDTTNQTFCIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNN 420

Query: 421 VKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           VKNHASYAFDSYYQKEGKT+GSCDFKG+AMITTTDPS  S +
Sbjct: 421 VKNHASYAFDSYYQKEGKTSGSCDFKGLAMITTTDPSHGSCI 462

[Close]

BLAST of Lsi02G005930 vs. NCBI nr
Match: TYJ99350.1 (glucan endo-1,3-beta-glucosidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 865.1 bits (2234), Expect = 2.7e-247
Identity = 428/462 (92.64%), Postives = 443/462 (95.89%), Query Frame = 0


Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60
           MADSK SLFFLFLFVFLT SEGKF PLI G KVEKDDKQPFVGVNIGSDVS LLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFVFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSKLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVA 120
           SFL FQKITHIRLYDTNPDILKAL GSKIRV+ISVPNNQLLAIG SN TAAAWI RNVVA
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 HYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
           +YPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYL 360

Query: 361 LHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNN 420
           LHVSGSGTFLANDTTNQT+CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNN
Sbjct: 361 LHVSGSGTFLANDTTNQTFCIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNN 420

Query: 421 VKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           VKNHASYAFDSYYQKEGKT+GSCDFKG+AMITTTDPS  S +
Sbjct: 421 VKNHASYAFDSYYQKEGKTSGSCDFKGLAMITTTDPSHGSCI 462

[Close]

BLAST of Lsi02G005930 vs. TAIR 10
Match: AT1G11820.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 741.5 bits (1913), Expect = 4.1e-214
Identity = 358/455 (78.68%), Postives = 411/455 (90.33%), Query Frame = 0


Query: 8   LFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLVSFLQFQK 67
           LFF F  + ++A+      L    KV++ DK PFVG NIG+DVSNLLSP++LV FLQ QK
Sbjct: 13  LFFFFTLLLISANSSS---LSHNIKVQEQDKDPFVGFNIGTDVSNLLSPTELVKFLQAQK 72

Query: 68  ITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVAHYPQTLI 127
           + H+RLYD +P++LKAL+ +K+RVIISVPNNQLLAIGSSN+TAA+WIGRNVVA+YP+TLI
Sbjct: 73  VNHVRLYDADPELLKALAKTKVRVIISVPNNQLLAIGSSNSTAASWIGRNVVAYYPETLI 132

Query: 128 SGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQA 187
           + ISVGDEVLTT+PS++PLLLPAIESLYNALVA+NL TQI VSTPHAASI+LD FPPSQA
Sbjct: 133 TAISVGDEVLTTVPSSAPLLLPAIESLYNALVASNLHTQIKVSTPHAASIMLDTFPPSQA 192

Query: 188 FFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEMVDP 247
           +FNQ+    ++PLLQFLSKTGSPLMMN YPYYV+MQNKGVVPLDN LF+PLTPSKEMVDP
Sbjct: 193 YFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYVYMQNKGVVPLDNCLFEPLTPSKEMVDP 252

Query: 248 NTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSN 307
           NTLLHYTNVLDAM+D+AY SMKNLN++DV VLVTESGWPSKGDSKEPYA+IDNADT+NSN
Sbjct: 253 NTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLVTESGWPSKGDSKEPYATIDNADTYNSN 312

Query: 308 LIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYLLHVSGSG 367
           LIKH+ DR+GTP HPE+TSSVYIYELFNEDLR+PPVSEA+WGLFYGNSTPVYLLHVSGSG
Sbjct: 313 LIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRAPPVSEASWGLFYGNSTPVYLLHVSGSG 372

Query: 368 TFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASY 427
           TFLANDTTNQTYCIAMD  DAKTLQ ALDWACGPG++NC+EIQPGE+CYQPNNVK HAS+
Sbjct: 373 TFLANDTTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASF 432

Query: 428 AFDSYYQKEGKTAGSCDFKGVAMITTTDPSEFSSL 463
           AF+SYYQKEG+ +GSCDFKGVAMITTTDPS  S +
Sbjct: 433 AFNSYYQKEGRASGSCDFKGVAMITTTDPSHGSCI 464

[Close]

BLAST of Lsi02G005930 vs. TAIR 10
Match: AT1G11820.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 575.1 bits (1481), Expect = 5.1e-164
Identity = 281/361 (77.84%), Postives = 325/361 (90.03%), Query Frame = 0


Query: 8   LFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLVSFLQFQK 67
           LFF F  + ++A+      L    KV++ DK PFVG NIG+DVSNLLSP++LV FLQ QK
Sbjct: 13  LFFFFTLLLISANSSS---LSHNIKVQEQDKDPFVGFNIGTDVSNLLSPTELVKFLQAQK 72

Query: 68  ITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVAHYPQTLI 127
           + H+RLYD +P++LKAL+ +K+RVIISVPNNQLLAIGSSN+TAA+WIGRNVVA+YP+TLI
Sbjct: 73  VNHVRLYDADPELLKALAKTKVRVIISVPNNQLLAIGSSNSTAASWIGRNVVAYYPETLI 132

Query: 128 SGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQA 187
           + ISVGDEVLTT+PS++PLLLPAIESLYNALVA+NL TQI VSTPHAASI+LD FPPSQA
Sbjct: 133 TAISVGDEVLTTVPSSAPLLLPAIESLYNALVASNLHTQIKVSTPHAASIMLDTFPPSQA 192

Query: 188 FFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEMVDP 247
           +FNQ+    ++PLLQFLSKTGSPLMMN YPYYV+MQNKGVVPLDN LF+PLTPSKEMVDP
Sbjct: 193 YFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYVYMQNKGVVPLDNCLFEPLTPSKEMVDP 252

Query: 248 NTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSN 307
           NTLLHYTNVLDAM+D+AY SMKNLN++DV VLVTESGWPSKGDSKEPYA+IDNADT+NSN
Sbjct: 253 NTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLVTESGWPSKGDSKEPYATIDNADTYNSN 312

Query: 308 LIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYLLHVSGSG 367
           LIKH+ DR+GTP HPE+TSSVYIYELFNEDLR+PPVSEA+WGLFYGNSTPVYLLHVSGSG
Sbjct: 313 LIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRAPPVSEASWGLFYGNSTPVYLLHVSGSG 370

Query: 368 T 369
           T
Sbjct: 373 T 370

[Close]

BLAST of Lsi02G005930 vs. TAIR 10
Match: AT2G01630.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 514.2 bits (1323), Expect = 1.1e-145
Identity = 247/447 (55.26%), Postives = 336/447 (75.17%), Query Frame = 0


Query: 11  LFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLVSFLQFQKITH 70
           L LF+FL AS                 +   +GVNIG++V+N+ SP+ +V+ L+ Q I  
Sbjct: 5   LLLFLFLFASSAL-------------SQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINR 64

Query: 71  IRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVAHYPQTLISGI 130
           +RLYD +  +L A + + ++VIISVPN+QLL I  SNATAA W+ RNV A+YP T I+ I
Sbjct: 65  VRLYDADRSMLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTI 124

Query: 131 SVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQAFFN 190
           +VG EVLT++ +A+ +L+ A++ +  ALV ANL  QI VSTPH+++IILD FPPSQAFFN
Sbjct: 125 AVGSEVLTSLTNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN 184

Query: 191 QSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEMVDPNTL 250
           ++    ++PLL+FL  TGSPL++N YPY+ ++Q+ GV+PLD ALF+PL  +KE VD NTL
Sbjct: 185 KTWDPVIVPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTL 244

Query: 251 LHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSNLIK 310
           LHYTNV DA++D+AYF+M  LN T++ ++VTESGWPSKG   E  A+++NA+T+NSNLI+
Sbjct: 245 LHYTNVFDAIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQ 304

Query: 311 HILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYLLHVSGSGTFL 370
           H+++++GTP HP    + YIYEL+NED R  PVSE NWGLFY N TPVY L ++G+G  L
Sbjct: 305 HVINKTGTPKHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAIL 364

Query: 371 ANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFD 430
           ANDTTNQT+CIA ++ D K LQ ALDWACGPGK +C+ +  GE+CY+P++V  H++YAF+
Sbjct: 365 ANDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFN 424

Query: 431 SYYQKEGKTAGSCDFKGVAMITTTDPS 458
           +YYQK GK +GSCDFKGVA +TTTDPS
Sbjct: 425 AYYQKMGKASGSCDFKGVATVTTTDPS 438

[Close]

BLAST of Lsi02G005930 vs. TAIR 10
Match: AT1G66250.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 511.1 bits (1315), Expect = 9.1e-145
Identity = 250/454 (55.07%), Postives = 339/454 (74.67%), Query Frame = 0


Query: 4   SKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLVSFL 63
           S L L  L L + + AS    PP          D+  ++GVNIG+D+S++  P+ +V+ L
Sbjct: 3   SLLHLLLLSLSLLVLASASPSPPA---------DEGSYIGVNIGTDLSDMPHPTQVVALL 62

Query: 64  QFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVAHYP 123
           + Q+I HIRLY+ +P +L AL+ + I+VIIS+PN+QLL IG SN+TAA W+ RNV+AHYP
Sbjct: 63  KAQEIRHIRLYNADPGLLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYP 122

Query: 124 QTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFP 183
            T+I+ +SVG EVLT++ +A+P+L+ AI++++ AL++ANL   I VSTP + S+ILDPFP
Sbjct: 123 ATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFP 182

Query: 184 PSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKE 243
           PSQAFFN+SL   ++PLL FL  T S LM+N YPY  +MQ+ GV+PLD ALFKP+ P+KE
Sbjct: 183 PSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKE 242

Query: 244 MVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDNADT 303
            VD NTL+ Y+N  DAM+D+ YF+M  LN T++ VLVTESGWPSKG++ EP A++DNA+T
Sbjct: 243 AVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANT 302

Query: 304 FNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYLLHV 363
           +NSNLI+H+L+++GTP  P +  S YIYEL+NED ++  +SE NWGLF  N  PVY+L +
Sbjct: 303 YNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDTKA-GLSEKNWGLFNANGEPVYVLRL 362

Query: 364 SGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKN 423
           + SG+ LANDTTNQTYC A +  D K LQ ALDWACGPGK +C+ I+ GE CY+P+NV  
Sbjct: 363 TNSGSVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVA 422

Query: 424 HASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPS 458
           HA+YAFD+YY + G    +C+F GVA ITTTDPS
Sbjct: 423 HANYAFDTYYHQTGNNPDACNFNGVASITTTDPS 446

[Close]

BLAST of Lsi02G005930 vs. TAIR 10
Match: AT2G01630.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 439.9 bits (1130), Expect = 2.6e-123
Identity = 215/395 (54.43%), Postives = 292/395 (73.92%), Query Frame = 0


Query: 11  LFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGVNIGSDVSNLLSPSDLVSFLQFQKITH 70
           L LF+FL AS                 +   +GVNIG++V+N+ SP+ +V+ L+ Q I  
Sbjct: 5   LLLFLFLFASSAL-------------SQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINR 64

Query: 71  IRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGSSNATAAAWIGRNVVAHYPQTLISGI 130
           +RLYD +  +L A + + ++VIISVPN+QLL I  SNATAA W+ RNV A+YP T I+ I
Sbjct: 65  VRLYDADRSMLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTI 124

Query: 131 SVGDEVLTTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQAFFN 190
           +VG EVLT++ +A+ +L+ A++ +  ALV ANL  QI VSTPH+++IILD FPPSQAFFN
Sbjct: 125 AVGSEVLTSLTNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN 184

Query: 191 QSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEMVDPNTL 250
           ++    ++PLL+FL  TGSPL++N YPY+ ++Q+ GV+PLD ALF+PL  +KE VD NTL
Sbjct: 185 KTWDPVIVPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTL 244

Query: 251 LHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSNLIK 310
           LHYTNV DA++D+AYF+M  LN T++ ++VTESGWPSKG   E  A+++NA+T+NSNLI+
Sbjct: 245 LHYTNVFDAIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQ 304

Query: 311 HILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVYLLHVSGSGTFL 370
           H+++++GTP HP    + YIYEL+NED R  PVSE NWGLFY N TPVY L ++G+G  L
Sbjct: 305 HVINKTGTPKHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAIL 364

Query: 371 ANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKAN 406
           ANDTTNQT+CIA ++ D K LQ ALDWACGPGK +
Sbjct: 365 ANDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVD 386

The following BLAST results are available for this feature:

BLAST of Lsi02G005930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65399	5.8e-213	78.68	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q9ZU91	1.5e-144	55.26	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q9C7U5	1.3e-143	55.07	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]
Q94CD8	4.0e-121	50.60	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
Q8VYE5	4.0e-89	38.12	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]

BLAST of Lsi02G005930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LX25	4.7e-250	93.51	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G062370 PE=3...	[more]
A0A6J1CDL3	1.4e-249	93.52	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010711 ...	[more]
A0A1S3B1W0	7.5e-248	92.64	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103485208 PE=3 SV...	[more]
A0A5A7UQD8	7.5e-248	92.64	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
E5GC60	7.5e-248	92.64	(1->3)-beta-glucan endohydrolase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]

BLAST of Lsi02G005930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895143.1	6.7e-251	94.16	glucan endo-1,3-beta-glucosidase 1 [Benincasa hispida]	[more]
XP_004145115.1	9.7e-250	93.51	glucan endo-1,3-beta-glucosidase 1 [Cucumis sativus] >KGN64511.1 hypothetical pr...	[more]
XP_022139909.1	2.8e-249	93.52	glucan endo-1,3-beta-glucosidase 1 [Momordica charantia] >XP_022139910.1 glucan ...	[more]
XP_008440956.1	1.6e-247	92.64	PREDICTED: glucan endo-1,3-beta-glucosidase 1 [Cucumis melo] >XP_008440965.1 PRE...	[more]
TYJ99350.1	2.7e-247	92.64	glucan endo-1,3-beta-glucosidase 1 [Cucumis melo var. makuwa]	[more]

BLAST of Lsi02G005930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11820.2	4.1e-214	78.68	O-Glycosyl hydrolases family 17 protein	[more]
AT1G11820.1	5.1e-164	77.84	O-Glycosyl hydrolases family 17 protein	[more]
AT2G01630.1	1.1e-145	55.26	O-Glycosyl hydrolases family 17 protein	[more]
AT1G66250.1	9.1e-145	55.07	O-Glycosyl hydrolases family 17 protein	[more]
AT2G01630.2	2.6e-123	54.43	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 378..462 e-value: 5.0E-43 score: 158.9
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 379..450 e-value: 1.1E-20 score: 74.1
None	No IPR available	GENE3D	1.20.58.1040		coord: 372..462 e-value: 2.9E-12 score: 48.9
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 41..368 e-value: 2.0E-106 score: 357.6
None	No IPR available	PANTHER	PTHR32227:SF120	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 1	coord: 18..394
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 42..361 e-value: 8.7E-73 score: 245.6
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 276..289
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 18..394
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 42..361

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G005930.1	Lsi02G005930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds